ライフサイエンスコーパス: DNA content

1 s using flow cytometric analysis of cellular DNA content.

2 s, rather than a general response to altered DNA content.

3 tidylserine externalization, and hypodiploid DNA content.

4 based on their greater than 6N (termed 6N+) DNA content.

5 n a parABI-deleted strain also increased the DNA content.

6 ithin the cell cycle based on its length and DNA content.

7 ermine nuclear size to a greater extent than DNA content.

8 rates per protein regardless of the cellular DNA content.

9 megakaryocytes and erythroblasts had normal DNA content.

10 chromatin compaction rather than changes in DNA content.

11 increases are not caused by a higher nuclear DNA content.

12 elements comprising roughly 11% of its total DNA content.

13 ndent manner, and resulted in cells with >4N DNA content.

14 ell density was determined by measurement of DNA content.

15 easily correlate to numbers of parasites or DNA content.

16 c release, nuclear fragmentation, and sub-G1 DNA content.

17 The primary endpoint was median %5-mC DNA content.

18 vity, cardiolipin content, and mitochondrial DNA content.

19 ve sequenced legume plastid genomes in novel DNA content.

20 to an accumulation of cells with a G(0)/G(1) DNA content.

21 ct polymorphisms present at <1% of the total DNA content.

22 esults in cell filamentation, with polyploid DNA content.

23 growth conditions that alter cell volume and DNA content.

24 dramatic increase in megakaryocyte size and DNA content.

25 s that include heterochromatin or repetitive DNA content.

26 hable from those of WT but were deficient in DNA content.

27 ogenase, PGC1alpha, CoxII, and mitochondrial DNA content.

28 -EGF shedding --> EGFR --> ERK --> increased DNA content.

29 in ERK phosphorylation, HB-EGF shedding, and DNA content.

30 essed by DNA/wet weight of brain and protein/DNA content.

31 DNA flow cytometry was used to measure DNA content.

32 uo2 arrest in prometaphase of PMII with a 2C DNA content.

33 of mitotic cells with characteristic G(2)/M DNA content.

34 ntiated by changes in mitochondrial mass and DNA content.

35 ic failure and accumulation of cells with 4n DNA content.

36 nt the propagation of cells with an abnormal DNA content.

37 hypertrophy before a change in cell size and DNA content.

38 ondrial metabolic activity and intracellular DNA content.

39 reak compared with those with larger nuclear DNA content.

40 lyploidy is defined as an increase in genome DNA content.

41 cogenic fungal biomass as quantified by Tri5 DNA content.

42 the proportion of cells with >4 C-value (C) DNA content.

43 ging properties of the cell such as size and DNA content.

44 ons in nuclear size occur without changes in DNA content.

45 hotgun sequencing reads) of their respective DNA content.

46 on both cellular DNA replication and nuclear DNA content.

47 e measured intensities essentially reflected DNA content.

48 (-70%) or overestimation (+160%) of species DNA contents.

49 structions of chromosome numbers and nuclear DNA contents.

50 -deficient horizontal cells display elevated DNA content (5N-34N) that varied continuously, suggestin

51 ls with an abnormal morphology and increased DNA content accumulated.

52 le the first provides information on nuclear DNA contents across land plants and some algal groups, t

53 but greater T cell receptor-excision circle DNA content after 48 weeks, despite similar virologic re

54 chondrial numbers per cell and mitochondrial DNA content, all of which increased after exposure to NO

55 ng a high proportion of cells with a haploid DNA content, an unprecedented state for trypanosomes.

56 interrogate larger cell populations by using DNA content analyses, a surprising result was obtained:

57 ay was verified using a molecular marker and DNA content analyses.

58 ase-pulse experiments with BrdU and EdU, and DNA content analysis indicate that uhrf1 mutant cells un

59 Detection of NH-MM DNA content and >/=1% PCs in S-phase were of independent

60 a quiescent G0 state, characterized by a 2N DNA content and a lower RNA content than G1 cells.

61 e maturation was verified by the increase in DNA content and adhesion to extracellular matrix protein

62 e (AD) neurons characterized by increases in DNA content and amyloid precursor protein (APP) gene cop

63 proliferating cells and display increases in DNA content and apoptosis, as well as mitotic spindle de

64 Myocytes show a diploid DNA content and carry, at most, two sex chromosomes.

65 lls results in dramatic increases in nuclear DNA content and cell and nucleolar size, whereas dMnt ov

66 , which undergo endoreplication to increment DNA content and cell size.

67 Amphibian genomes differ greatly in DNA content and chromosome size, morphology, and number.

68 To more accurately estimate whole-genome DNA content and compare these estimates to newly assembl

69 and arrested cells have similar patterns of DNA content and cyclin expression, a large fraction of t

70 , and by combining it with detection of both DNA content and DNA replication, this method allows uneq

71 monstrated that there were increased triplex DNA content and double-stranded breaks in ChlR1-depleted

72 re coupled with alterations in mitochondrial DNA content and expression of genes associated with extr

73 Mitochondrial DNA content and expression of genes involved in multiple

74 brid cells that maintain a stable tetraploid DNA content and have morphology, growth rate, and antige

75 cts fundamental cellular properties, such as DNA content and intracellular localization.

76 rated that hYVH1 expression affects cellular DNA content and is a novel cell survival phosphatase pre

77 Further, DNA content and messenger RNA expression of osteogenic (

78 to examine the cell and nucleoid morphology, DNA content and metabolic activity.

79 ell cycle progression resulting in increased DNA content and multinucleation.

80 A diploid DNA content and only two chromosomes 12 were found in ne

81 mely variable and undergo dynamic changes in DNA content and organization.

82 We analyzed the DNA content and proliferation index of bone marrow plasm

83 o increases the proportion of cells with >4C DNA content and promotes repeat mutagenesis.

84 Here we demonstrate label-free prediction of DNA content and quantification of the mitotic cell cycle

85 as determined by flow cytometry analysis of DNA content and quantitation of the proportion of cells

86 were associated with decreased mitochondrial DNA content and reduced expression of mitochondria-encod

87 as also accompanied by reduced mitochondrial DNA content and reduced mitochondrial mRNA.

88 eration, reduced the number of cells with 4n DNA content and rescued expression of FoxM1 target genes

89 e bypassed so that the cell can increase its DNA content and size.

90 ed predominantly of myocytes with 2n diploid DNA content and that tetraploid and octaploid nuclei con

91 , short DNA fragment lengths, low endogenous DNA content and the potential for modern contamination.

92 w that isogenic, isotypic cells of identical DNA content and the same cell-cycle phase can still disp

93 rrangements that explain a rapid increase in DNA content and trigger breakage-fusion-bridge cycles.

94 low sorting of single nuclei on the basis of DNA content and whole-genome amplification (WGA); this i

95 melanoma cells is correlated with increased DNA content and, reciprocally, that the least reactive c

96 TGF-beta signaling, we compared the HIV RNA/DNA contents and cellular transcriptomes of CD103(+) and

97 ts deletion gave rise to cells with aberrant DNA contents and increased volumes.

98 iochemical (collagen, glycosaminoglycan, and DNA content) and biomechanical (tensile and compressive)

99 sly measure the phagocytic index, macrophage DNA content, and 5-ethynyl-2'-deoxyuridine (EdU) incorpo

100 increase in exocrine pancreas size, protein/DNA content, and acinar proliferation were all blocked i

101 The arrested cells have a 4N DNA content, and addition of caffeine causes immediate e

102 d by increased annexin V staining, decreased DNA content, and appearance as assessed by transmission

103 ctivity, Mitotracker staining, mitochondrial DNA content, and cellular oxygen respiration.

104 ases mitochondrial biogenesis, mitochondrial DNA content, and glucose uptake in subcutaneous adipose

105 They also exhibit aberrations in morphology, DNA content, and growth characteristics compared with WT

106 viability, causes cell cycle arrest with 4N DNA content, and leads to apoptosis.

107 or absence of LPD, surface maker expression, DNA content, and microsatellite polymorphisms, 74 had di

108 sess mitochondrial morphology, mitochondrial DNA content, and mitochondrial enzyme activities.

109 component cardiolipin, of the mitochondrial DNA content, and of the mitochondrial DNA replication an

110 mors, flow-sorting genomic subpopulations by DNA content, and profiling genomes using comparative gen

111 cells (binding of 31 antibodies, viability, DNA content, and relative cell size).

112 onse, exhibited an increase in mitochondrial DNA content, and required oxidative phosphorylation to m

113 cellular volume, even with the same absolute DNA content, and so must compensate for differences in D

114 rrest in G2 of the cell cycle with increased DNA content, and that nutrient availability triggers pro

115 tochondrial enzyme activities, mitochondrial DNA content, and the number and size of mitochondria.

116 f photosynthetic pigments and polyphosphate, DNA content, and the rate of translation.

117 uncoupled oxygen consumption, mitochondrial DNA content, and voltage-dependent anion channel protein

118 y (approximately 9 kb/gene) and lower repeat DNA content (approximately 13.1%) in Brachypodium when c

119 features of transporter activity as well as DNA content are determinants of SP/NSP identity.

120 d or polytene cells, which have more than 2C DNA content, are widespread throughout nature and presen

121 Cells with abnormal DNA content arose in the population, and replication was

122 Embryos with DNA contents around this value show intermediate cell cy

123 es of endoreduplication, with some attaining DNA contents as high as 96C and 192C.

124 idy (state of abnormal chromosome number and DNA content) at the next mitosis since extra centrosomes

125 mmediately arrested cells at a status of 4 N DNA content, B19V-infected 4 N cells still incorporated

126 Genes can be mutated by altering DNA content (base changes) or DNA length (insertions or

127 DNA content based flow sorting of tumor cells followed b

128 demonstrates a novel alternative to existing DNA content-based approaches for sorting cardiomyocytes

129 We applied DNA content-based flow sorting to identify and isolate t

130 death, such as an increase in cells with 4N DNA content before the appearance of cells with <2N DNA

131 uncover substantial differences in regional DNA content between inbred strains of mice.

132 Its outputs permit estimation of DNA content between mapped loci and help to create an in

133 DCV)" characterized by an increased range of DNA content both in cell populations and within single c

134 requires the precise duplication of cellular DNA content but also of membranes and organelles.

135 that myofiber transcription is responsive to DNA content but uncoupled from cell size during hypertro

136 o morphology, major structural proteins, and DNA content but were noninfectious.

137 in embryos increased postnatal day 10 brain DNA content by 28%, suggesting a role for IGF-1 in brain

138 The detection of changes in nuclear DNA content by correlating color recovery of H2B::mEosFP

139 ovel method to measure the relative telomere DNA content by dot blot analysis.

140 Analysis of their DNA content by flow cytometry, as well as chromosome cou

141 aging of individual cells by measuring their DNA content by fluorescence microscopy.

142 in which most cardiomyocytes increase their DNA content by multinucleation and nuclear polyploidizat

143 ring of endocycles results in higher nuclear DNA content (C value) that in some cases has been correl

144 ls and that an increase in human-specific L1 DNA content can be detected in the brains of normal cont

145 Markers of cell cycle progression based on DNA content cannot distinguish between mitotic cardiomyo

146 s in vitro by annexin V staining, subdiploid DNA content, caspase activation, and loss of mitochondri

147 VDase) activity and by measuring nucleosomal DNA content (cell death ELISA).

148 tively understanding the relationships among DNA content, cell size, and gene expression variability

149 f the host plant, had deoxyribonucleic acid (DNA) contents, cellular sizes and survival rates similar

150 ranscripts results in slowed growth, altered DNA content, changes in cell morphology, and increased s

151 duplication events that fundamentally alter DNA content, chromosome number, and gene dosage.

152 of data representing cellular doubling time, DNA content, chromosome number, metacentric chromosome f

153 ta1 scaffolds exhibited a trend of increased DNA content compared with unstimulated EPC scaffolds.

154 ncreased number of centrosomes and increased DNA content, compared to Tsc1(+/+) cells.

155 G1/S boundary were slower to reach full G2/M DNA content, consistent with a delay in S phase.

156 Flow cytometry revealed an increased 4N DNA content, consistent with a G2 arrest.

157 rofile whereby a majority of cells have a 4N DNA content, consistent with the onset of G2 arrest.

158 ed the ability to proliferate with increased DNA content despite the presence of functional p53.

159 DNA content distribution histograms showed S-phase block

160 mutants exit macronuclear S with a wild-type DNA content, division of the amitotic macronucleus is bo

161 nts to show that a 16-fold change in nuclear DNA content does not influence the relative size of the

162 The DNA content, epitope 846, COL2(IX), and the denaturation

163 ents and quantitative PCR (qPCR) of telomere DNA content, expressed as the ratio of telomeric product

164 e targets for this lethal cancer, we applied DNA content flow cytometry to a series of 15 tumor sampl

165 ing, fluorescence in situ hybridization, and DNA content flow cytometry.

166 uces the accumulation of cells with > or =4N DNA content, followed by cell death.

167 arrest, a medium hypomorph arrested with 4N DNA content, followed later by apoptosis, and a strong P

168 ncreatic mass were paralleled by protein and DNA content following camostat feeding and rapamycin adm

169 ting (disassembly) of NCs to expose their RC DNA content for conversion to CCC DNA.

170 of endoreplication, and blt mutants uncouple DNA content from morphogenesis in mutants with increased

171 , suggesting an underlying scaling law where DNA-content functions as a limiter of muscle cell size.

172 d results in the endoreduplication (cellular DNA content >4N) of MCF-7 and MDA MB-231 cells.

173 Finally, changes in repetitive DNA content, guanine-cytosine isochore structure, and nu

174 Telomere length/DNA content has been measured in epidemiological/clinica

175 absence of detectable increases in cellular DNA content however, it has been difficult to directly d

176 t, and arrested in a G(1)-like state with 4N DNA content in a p53-dependent manner.

177 Analysis of repeat DNA content in BESs revealed that approximately 15.42% o

178 We detected reduced mitochondrial DNA content in biopsies of heterotaxy patients.

179 T cell counts, lower plasma viremia, and SIV-DNA content in blood and LN compared to NCs, but had sim

180 er levels of residual plasma viremia and SIV-DNA content in blood and tissues.

181 tosine (%-5hmC) and 5-methylcytosine (%-5mC) DNA content in blood collected at birth (n=306), early c

182 sed device is sufficient to measure cellular DNA content in both fixed and living tumor cells.

183 ulted in the accumulation of abnormally high DNA content in cells over time (hyperploid).

184 The DNA content in cells sorted by fluorescent tissue-specif

185 progenitor cell proliferation and a reduced DNA content in endoreduplicating trophoblast giant cells

186 link in coordinating cell shape and nuclear DNA content in endoreplicated Arabidopsis trichomes.

187 th regard to measurements of telomere length/DNA content in epidemiological/clinical circumstances.

188 idium homodimer-2) to detect both lipoid and DNA content in individual particles.

189 with a Pyk2 kinase inhibitor increased viral DNA content in keratinocytes that maintain viral episome

190 ization and estimation of changes in nuclear-DNA content in live cells during their development has n

191 hosphorylation, and longer term increases in DNA content in mesangial cells.

192 s were utilized to demonstrate that aberrant DNA content in RB-deficient cells occurs concomitantly w

193 Myofibers increase size and DNA content in response to a hypertrophic stimulus, thus

194 cells, FANCJ-null (FA-J) cells accumulate 4N DNA content in response to DNA interstrand crosslinks (I

195 To scan for gene and repeat DNA content in the libraries, end-sequencing was perform

196 Whole-genome duplication (WGD) doubles the DNA content in the nucleus and leads to polyploidy.

197 ed by Western blot comparison with the viral DNA content in the tumors.

198 umber of cells is lower than normal, and the DNA content in these cells is significantly increased.

199 have focused on measurements of the relative DNA content in tumor cells compared to normal cells and

200 omes with similar methylation and repetitive DNA content, including those from crops and mammals.

201 On average, DNA content increased by approximately 250 megabases, of

202 evidenced by accumulation of cells with 4 n DNA content, increased mitotic index, separated centroso

203 lei displayed large variability with average DNA content increases of ~8% over non-diseased controls

204 ith flow cytometric determination of nuclear DNA content indicated near perfect agreement between the

205 leads to the accumulation of cells that have DNA content intermediate to 2N and 4N in proliferating t

206 disassembly (uncoating) to deliver their RC DNA content into the host cell nucleus for conversion to

207 potential (DeltaPsi(m) loss) in relation to DNA content is also demonstrated.

208 e cell cycle even in 2-cell embryos when the DNA content is increased.

209 In each dot, the DNA content is measured by a DNA stain (Dx) and the telo

210 asured by a DNA stain (Dx) and the telomeric DNA content is measured with a telomeric probe (T).

211 k of correlation between guard cell size and DNA content, lack of arabinans in cell walls, and perpet

212 ors resulted in accumulation of MKs with low DNA content levels and significant reduction of higher p

213 known ploidy status either by karyotyping or DNA content measurement using flow cytometry.

214 DNA content measurement, real-time polymerase chain reac

215 a, NRF-1, Tfam and CytC genes, mitochondrial DNA content, mitochondrial activity and mitochondrial me

216 ism measures the ratio of cellular volume to DNA content, most likely through sequestration of a tran

217 ; instead, the twofold overall difference in DNA content must reflect locally operating forces betwee

218 sation achieved can be judged by the uniform DNA content, narrow size distribution, synchronous divis

219 ontrast to the twofold overall difference in DNA content, no disparity in size was observed for this

220 ycle pause in Drosophila is about 70% of the DNA content normally present at cycle 14.

221 The typical increase in myofiber DNA content observed at the later stage of hypertrophy w

222 ed next generation sequencing to analyze the DNA content of ALT telomeres.

223 homes, which are single cells with a nuclear DNA content of approximately 16C to 32C.

224 rise to ventral cord neurons with an average DNA content of approximately 2.5 n.

225 The DNA content of dnaE mutants increased about eightfold in

226 chromosome rearrangements to manipulate the DNA content of embryos, we determined that the threshold

227 n, that constitute a large percentage of the DNA content of eukaryotic genomes.

228 The DNA content of eukaryotic nuclei (C-value) varies approx

229 e same method is effective in predicting the DNA content of fission yeast, it is likely to have a bro

230 was present between H(f), and the repetitive DNA content of five eukaryotic genomes previously determ

231 The DNA content of mature endosperm increased 43% upon RBR1

232 The DNA content of mature stalk cells suggests that they als

233 Unexpectedly, the DNA content of methylglyoxal-derived imidazopurinone and

234 To determine how the DNA content of one nucleus is communicated to the other,

235 xpression of DN-HSF1 dramatically alters the DNA content of PC-3 cells (derived from p53 null prostat

236 asured by [3H]thymidine incorporation and by DNA content of the cultures.

237 Analysis of the DNA content of the mutant neurons indicates that defecti

238 eightfold in 4 h at 40 degrees C, as did the DNA content of the suppressed strains.

239 vesicle cytokine responses, whereas the CpG DNA content of vesicles did not.

240 on phenotype of seqA mutants and reduced the DNA content of wild-type strains; virtually identical ef

241 Hybrids with DNA contents of approximately 2n, 3n, and 4n were observ

242 d pancreatic mass, independent of changes in DNA content or cell proliferation in mice.

243 Large cells are produced by increasing DNA content or ploidy, a developmental strategy employed

244 GLP-1R agonists did not increase pancreatic DNA content or the number of Ki67(+) cells in the exocri

245 Although blt mutants have normal trichome DNA content, overexpression of BLT results in an additio

246 GAA treatment significantly reduces genomic DNA content (P < 0.0001) and creates an increased potent

247 Established biomarkers (abnormal DNA content, p53, and cyclin A expression) and new bioma

248 aining of isolated plastids to determine the DNA content per plastid for seedlings grown in the dark

249 Thus, the DNA content per plastid is not constant but varies durin

250 the DNA paralleled a progressive decrease in DNA content per plastid.

251 DNA content profiling of dcd1Delta cells differs from an

252 smooth muscle cell (CASMC) number, cellular DNA content, protein synthesis, and PCNA staining.

253 concentration-dependent increase in cellular DNA content, protein synthesis, cell number, and prolife

254 cally, immunohistochemistry and the residual DNA content quantified.

255 ted for all 10 sorghum chromosomes and their DNA content quantified.

256 After ATI, SIV-DNA content rapidly increased in NCs, while it remained

257 A decrease in mitochondrial (mt)DNA content, rather than CI mutations, was the main caus

258 s deal with increased centrosome numbers and DNA content remains unknown.

259 S phase nuclei, flow-sorted on the basis of DNA content, replicative labeling was widely distributed

260 DNase I to dissolve NETs, which have a high DNA content, restored perfusion in the kidney and heart

261 d enlarged epidermal cells with an increased DNA content resulting from additional endocycles.

262 o a comparable level but the reduced overall DNA content results in significantly higher viability of

263 Knockdown of SIRT1 increases cellular abasic DNA content, sensitizing cells to death induced by genot

264 ometric analysis of DNA strand breaks versus DNA content showed that apoptosis induced by PEITC-NAC o

265 Flow cytometric analysis of DNA content showed that JNJ-7706621 delayed progression

266 Measurement of nuclear DNA contents showed that PL cells were haploid relative

267 ucleolin in relation to cell cycle position (DNA content) showed expression during G1-S and persisten

268 tent before the appearance of cells with <2N DNA content, suggesting a mixed response.

269 cancer cells also have greater-than-diploid DNA content, suggesting that polyploidy is a common prec

270 Nutlin-3a for 24 hours accumulated 2N and 4N DNA content, suggestive of G(1) and G(2) phase cell cycl

271 specimens containing potentially less target DNA content than specimens from symptomatic males.

272 repair and the maintenance of mitochondrial DNA content, the regulation and function of RRM2B in sen

273 patchy cell cycle behavior due to threshold DNA contents, the expression of these genes correlates t

274 ecies, provide information about euchromatic DNA content, they cannot give an accurate estimate of ge

275 th intact checkpoint function arrest with 4N DNA content, those with compromised checkpoint function

276 e is for the cell to amplify its chromosomal DNA content through endoreduplication cycles.

277 DNA yield: depletion of the human genomic (g)DNA content through hybridization with human gDNA baits,

278 Yet, cells fail to increase their DNA content to 4N as revealed by FACS analysis, probably

279 ell types become polyploid, increasing their DNA content to attain a large cell size.

280 s play crucial roles in equal segregation of DNA content to daughter cells, coordination of growth an

281 Cdc25B also causes cells with an S phase DNA content to enter mitosis prematurely in a p53-indepe

282 ic DNA and allows progressive delineation of DNA content to within a few hundred base pairs of a geno

283 orted into S-phase fractions on the basis of DNA content using flow cytometry.

284 al cortex brain cells were found to display "DNA content variation (DCV)" characterized by an increas

285 HIV DNA content was 15-fold higher in CD25(+) FoxP3(+) memor

286 e-sequence-positive SMCs, the average unique DNA content was approximately 6.5 Mb (range 0.3-22.2 Mb)

287 ells with an anomalous size distribution and DNA content was found in this population.

288 tage of EU-positive myonuclei; however, when DNA content was held constant by preventing myonuclear a

289 a significant increase in cell viability and DNA content were observed in PKCdelta knockdown McA cell

290 llular assays demonstrated increased triplex DNA content when RPA is transiently repressed, suggestin

291 ted in accumulation of cells with 4N or more DNA content, whereas coadministration of vorinostat mark

292 odazole, RB-proficient cells arrest with 4 n DNA content, whereas RB-deficient cells bypass the mitot

293 hain vibrations originating from protein and DNA contents, whereas the second was predominantly the g

294 wever, this study primarily measured the HIV DNA content, which also includes defective proviruses th

295 suggest that organisms with high repetitive DNA content, which include humans, could use similar dev

296 es detect CN changes relative to the overall DNA content, which is often not diploid in cancer.

297 cluding large differences in alpha-satellite DNA content, which may influence the fidelity and bias o

298 ested EPCs at a cell cycle status with a 4 N DNA content, which was previously claimed to be "G2/M ar

299 cyclin/p53(-/-) tumors showed a near-diploid DNA content with no aberrant centrosome numbers.

300 cell shapes, but the mechanism coordinating DNA content with shape and size remains obscure.