ライフサイエンスコーパス: DNA ligase

1 ed DNA by Chlorella virus DNA ligase (PBCV-1 DNA ligase).

2 incubation times than required when using T4 DNA ligase.

3 ith ligation of these strands mediated by T4 DNA ligase.

4 s six proteins: UvrA-D, DNA polymerase I and DNA ligase.

5 gth can be modulated by the concentration of DNA ligase.

6 nd the resulting termini are ligated with T4 DNA ligase.

7 or strategy that rewards exposure to nuclear DNA ligase.

8 n of linear DNA molecules in the presence of DNA ligase.

9 ulation of end-joining in the presence of T4 DNA ligase.

10 uclease 1 to DNA polymerase beta and then to DNA ligase.

11 of a 5' exonuclease, a DNA polymerase and a DNA ligase.

12 logous end-joining mechanism that utilizes a DNA ligase.

13 T5 exonuclease, Phusion DNA polymerase, and DNA ligase.

14 e intermediate is a common first step of all DNA ligases.

15 sis by DNA polymerases or sealing by classic DNA ligases.

16 DNA ligation reactions used by ATP-dependent DNA ligases.

17 A splint are notoriously poor substrates for DNA ligases.

18 AP) endonuclease 1, DNA polymerase beta, and DNA ligases.

19 PO4) and 5'-OH ends that cannot be sealed by DNA ligases.

20 g the mechanism and specificity of mammalian DNA ligases.

21 nd illustrates examples using the Taq and T4 DNA ligases.

22 by DNA exonucleases or ligated by T3 and T4 DNA ligases.

23 Yeast dna2 mutants, like mutants in DNA ligase 1 (cdc9), accumulate low molecular weight, na

24 To protect the integrity of the genome, DNA ligase 1 (LIG1) discriminates against DNA junctions

25 omosomal DSBs and raise the possibility that DNA ligase 1 (Lig1) may contribute more to A-EJ than pre

26 nt of UHRF1 by the replication machinery via DNA ligase 1 (LIG1).

27 evidence for an unanticipated sufficiency of DNA ligase 1 for these intra-chromosomal events.

28 The dual functions of DNA ligase 1 in replication and non-homologous end-joini

29 lved an essential and non-redundant role for DNA ligase 1 in the fusion of sister chromatids bearing

30 ow that the tomato (Solanum lycopersicum L.) DNA ligase 1 specifically and efficiently catalyzes circ

31 hamiana Domin plants in which the endogenous DNA ligase 1 was silenced.

32 is study we propose that PSTVd subverts host DNA ligase 1, converting it to an RNA ligase, for the fi

33 A's interaction with Flap endonuclease 1 and DNA Ligase 1, DNA metabolism enzymes.

34 ivity that may employ either DNA ligase 3 or DNA ligase 1.

35 NA polymerase delta, flap endonuclease 1 and DNA ligase 1.

36 Parp-1, DNA-ligases 1 (Lig1) and 3 (Lig3), and Xrcc1 are implica

37 DNA ligase 3 (Lig3) and its cofactor XRCC1 are widely co

38 ext of multiple genetic knockouts, including DNA ligase 3 and 4 double-knockouts.

39 end-joining activity that may employ either DNA ligase 3 or DNA ligase 1.

40 logous end-joining and is not compensated by DNA ligases 3 or 4.

41 RNA interference-mediated knockdown of DNA ligase 3alpha abolished resistance to apoptotic cell

42 s is associated with increased expression of DNA ligase 3alpha, poly(ADP-ribose) polymerase 1 (PARP1)

43 mplex (Ku) for DSB recognition and the XRCC4/DNA ligase 4 (Lig4) complex for ligation.

44 XRCC4 and DNA Ligase 4 (LIG4) form a tight complex that provides D

45 ne or more requisite C-NHEJ factors, such as DNA ligase 4 (Lig4) or XRCC4, end-joining during CSR occ

46 Lig4(R278H/R278H) (Lig4(R/R)) mouse model of DNA Ligase 4 (LIG4) syndrome, in which a hypomorphic Lig

47 To compare the specific contribution of DNA ligase 4 (LIG4), Artemis, and DNA-protein kinase cat

48 ing Ku heterodimer, XLF/Cernunnos, and XRCC4/DNA Ligase 4 (Lig4).

49 d for the recruitment of break-sealing XRCC4-DNA ligase 4 complex at DSB sites in induced pluripotent

50 s as a scaffold for the recruitment of XRCC4-DNA ligase 4 complex.

51 u70/80 complex for DSB recognition and XRCC4/DNA ligase 4 for ligation.

52 urthermore, mutation of required NHEJ factor DNA Ligase 4 results in enhanced haploid recovery.

53 Surprisingly, we found that DNA ligase 4, essential for NHEJ, did not make a signifi

54 r-chromosomal fusion events in cells lacking DNA ligase 4, in contrast to a remarkably consistent pro

55 demonstrated the fundamental contribution of DNA ligase 4-dependent classical non-homologous end-join

56 Ku or DNA ligase 4-independent alternative end joining (alt-EJ

57 ologous end-joining (NHEJ) factors 53BP1 and DNA ligase 4.

58 prevented the nuclear translocation of XRCC4-DNA ligase 4.

59 Specifically, TDG and DNA ligase activities are reduced by a 3'-flanking 8-oxo

60 4 (LIG4) form a tight complex that provides DNA ligase activity for classical non-homologous end joi

61 duction in the rate of pol beta synthesis or DNA ligase activity on any of the fragments bound to GR-

62 e I whereas LigIII is the only mitochondrial DNA ligase and is essential for the survival of cells de

63 es expressing BCR-ABL1 to the combination of DNA ligase and PARP inhibitors correlates with the stead

64 on of these cell lines with a combination of DNA ligase and PARP inhibitors inhibited ALT NHEJ and se

65 However, T4 DNA ligase and RtcA can use 3'-phosphorylated nicks in d

66 nuclear gene encodes the only mitochondrial DNA ligase and so is essential for this organelle.

67 n strategy which expliots the specificity of DNA ligase and the speed of isothermal amplification to

68 plications for the biological specificity of DNA ligases and functions of PARP-like zinc fingers.

69 Lig, is a conserved feature of ATP-dependent DNA ligases and GTP-dependent mRNA capping enzymes.

70 profiling of the substrate specificities of DNA ligases and illustrates examples using the Taq and T

71 velop small molecule inhibitors of mammalian DNA ligases and/or their functional protein partners tha

72 fferent nucleotide content parallels that of DNA ligase, and optimal ligation efficiency is attained

73 tidyltransferase superfamily of RNA ligases, DNA ligases, and RNA capping enzymes.

74 a 3'-5' DNA helicase; LIG, an ATP-dependent DNA ligase; and Exo, a metallo-beta-lactamase-family nuc

75 es the fact that defects or insufficiency in DNA ligase are casually linked to genome instability.

76 ajor DNA polymerases (Pol I and Pol III) and DNA ligase are directly involved with oligo recombinatio

77 rase B (PolB), flap endonuclease (Fen1), and DNA ligase are required to complete ribonucleotide proce

78 DNA ligases are a highly conserved group of nucleic acid

79 ryal, archaeal, and many bacterial and viral DNA ligases are ATP-dependent.

80 Our results demonstrate that the BER DNA ligases are compromised by subtle changes in all 12

81 Evidence suggesting that DNA ligases are essential for cell viability includes th

82 DNA ligases are essential guardians of genome integrity

83 DNA ligases are essential guardians of genomic integrity

84 All the eukaryotic DNA ligases are known to use adenosine triphosphate (ATP

85 DNA ligases are the sine qua non of genome integrity and

86 Using the Chlorella virus DNA ligase as a proof of principle, we recapitulate the

87 f base excision repair can be mediated by T4 DNA ligase as well as human DNA ligase I or ligase IIIal

88 nd an OB domain (these two are common to all DNA ligases) as well as a distinctive beta-hairpin latch

89 ASFV DNA ligase (AsfvLIG) is one of the most error-prone liga

90 he ligation fidelity of Thermus thermophilus DNA ligase at a range of temperatures, buffer pH and mon

91 rlying the coordination between pol beta and DNA ligase at the final ligation step to maintain the BE

92 ction instead of the existing chemical or T4 DNA ligase-based methods allows quantitative conversion

93 Whereas DNA ligase-based RASL assays suffer from extremely low a

94 Globular domains from both the human DNA ligase binding protein XRCC4 and bacteriophage varph

95 Crystal structures of DNA ligases bound to nucleotide and nucleic acid substra

96 ized plasmids, indicating that an additional DNA ligase can support NHEJ.

97 ed DNA structures with abnormal DNA termini, DNA ligase catalytic activity can generate and/or exacer

98 RNA and DNA ligases catalyze the formation of a phosphodiester b

99 DNA ligases catalyze the joining of DNA strands to compl

100 We apply DNA ligase-catalyzed cyclization kinetics experiments to

101 We describe the application of T4 DNA ligase-catalyzed DNA templated oligonucleotide polym

102 The development and in-depth analysis of T4 DNA ligase-catalyzed DNA templated oligonucleotide polym

103 We have developed a method for the T4 DNA ligase-catalyzed DNA-templated polymerization of 5'-

104 T4 DNA ligase catalyzes phosphodiester bond formation betwe

105 Chlorella virus DNA ligase (ChVLig) has pluripotent biological activity

106 Chlorella virus DNA ligase (ChVLig) is a minimized eukaryal ATP-dependen

107 Chlorella virus DNA ligase (ChVLig) is an instructive model for mechanis

108 nformational dynamics of the Chlorella virus DNA ligase (ChVLig), a minimized eukaryal ATP-dependent

109 The catalytic core of ATP-dependent DNA ligases consists of an N-terminal nucleotidyltransfe

110 The DNA ligase D (LigD) 3'-phosphoesterase (PE) module is a

111 e homodimeric DNA end-binding protein Ku and DNA ligase D (LigD), a modular enzyme composed of a C-te

112 ogous end-joining (NHEJ) catalysed by Ku and DNA ligase D (LigD).

113 e strand break (DSB) repair driven by Ku and DNA ligase D (LigD).

114 acterium tuberculosis LigD, an ATP-dependent DNA ligase dedicated to nonhomologous end joining, in co

115 eplication in the absence of the replicative DNA ligase, DNA ligase I.

116 The phage encodes its own primase, DNA ligase, DNA polymerase, and enzymes necessary to syn

117 of the enzyme helps coordinate the entry of DNA ligase during Okazaki fragment maturation.

118 Escherichia coli DNA ligase (EcoLigA) repairs 3'-OH/5'-PO4 nicks in duple

119 Paradoxically, when DNA ligases encounter nicked DNA structures with abnorma

120 Eukaryotes possess multiple DNA ligase enzymes, each having distinct roles in cellul

121 ts to generate cells devoid of mitochondrial DNA ligase failed.

122 RNA ligase 1; Rnl1) and the NAD(+)-dependent DNA ligase family (Escherichia coli LigA), captured as t

123 for mechanistic studies of the ATP-dependent DNA ligase family.

124 Next, DNA repair activities of DNA ligase, flap endonuclease and RNase H2 were monitore

125 trand phosphates at the outer margins of the DNA ligase footprint; (ii) essential contacts of Ser-41,

126 stereochemical preferences of AP endo and T4 DNA ligase for phosphorothioate substrates, we show that

127 notion that DNA ligase III (LIG3), the only DNA ligase found in mitochondria, is essential for viabi

128 DNA ligases have broad application in molecular biology,

129 Human DNA ligase I (hLigI) joins Okazaki fragments during DNA

130 Human DNA ligase I (hLigI) participates in DNA replication and

131 DNA ligase I (LIG1) catalyzes the ligation of single-str

132 (Pol delta), flap endonuclease 1 (FEN1) and DNA ligase I (Lig1).

133 (Pol delta), flap endonuclease 1 (FEN1) and DNA ligase I (LigI) that complete Okazaki fragment proce

134 (pol beta), flap endonuclease 1 (FEN1), and DNA ligase I (LigI).

135 DNA ligase I and DNA ligase III/XRCC1 complex catalyze t

136 idues reduced the in vitro ubiquitylation of DNA ligase I by Cul4-DDB1 and DCAF7.

137 Because DNA ligase I has been reported to be ubiquitylated, we u

138 tes and the presence of N-terminal domain of DNA ligase I in a coupled reaction governs the channelin

139 l redundancy between DNA ligase IIIalpha and DNA ligase I in excision repair.

140 nockdown of DCAF7 reduced the degradation of DNA ligase I in response to inhibition of proliferation

141 lication factor C, DNA polymerase delta, and DNA ligase I in the absence of DNA via its non-conserved

142 biquitylated lysine residues and showed that DNA ligase I interacts with and is targeted for ubiquity

143 azaki fragments by the flap endonuclease and DNA ligase I joins nascent fragments.

144 a template base and the N-terminal domain of DNA ligase I mediates its interaction with pol beta.

145 e mediated by T4 DNA ligase as well as human DNA ligase I or ligase IIIalpha-XRCC1 complex.

146 intermediates compromise the end joining by DNA ligase I or the DNA ligase IV/XRCC4 complex.

147 the enzymes flap endonuclease 1 (FEN-1) and DNA ligase I that complete the processing and joining of

148 k left after flap removal could be sealed by DNA ligase I to complete fragment joining.

149 Furthermore, APE1 stimulated DNA ligase I to resolve a long double-flap intermediate,

150 ta was used to measure repair synthesis, and DNA ligase I was used to seal the nick.

151 cleus, LigIII has functional redundancy with DNA ligase I whereas LigIII is the only mitochondrial DN

152 ropriate relative stoichiometry of FEN-1 and DNA ligase I, which compete for binding to proliferating

153 h to map ubiquitylation sites and screen for DNA ligase I-associated E3 ubiquitin ligases.

154 n the absence of the replicative DNA ligase, DNA ligase I.

155 PCNA clamp, its loader RFC, and completed by DNA ligase I.

156 roduction of nicks that could be sealed with DNA ligase I.

157 nce of polynucleotide kinase (PNK) and human DNA ligase III (Lig III).

158 le lines of evidence support the notion that DNA ligase III (LIG3), the only DNA ligase found in mito

159 Mammalian DNA ligase III (LigIII) functions in both nuclear and mi

160 ymerase-1, X-ray cross-complementing factor1-DNA ligase III and enzymes involved in processing 3'-blo

161 Human DNA ligase III has essential functions in nuclear and mi

162 ed for Iduna ubiquitination of PARP1, XRCC1, DNA ligase III, and KU70.

163 on with other experiments, demonstrated that DNA ligase III, but not ligase IV or ligase I, is primar

164 g PAR polymerase-1, 2 (PARP1, 2), nucleolin, DNA ligase III, KU70, KU86, XRCC1, and histones.

165 demonstrated high levels of PARylated Chd1L, DNA ligase III, SSrp1, Xrcc-6/Ku70, and Parp2 in pluripo

166 ere discovered, including PARP-1, hMutSbeta, DNA ligase III, XRCC1, and PNK.

167 DNA ligase I and DNA ligase III/XRCC1 complex catalyze the ultimate ligat

168 Elevated levels of DNA ligase IIIalpha (LigIIIalpha) have been identified a

169 with a FLT3 inhibitor demonstrate decreased DNA ligase IIIalpha and a reduction in DNA deletions, su

170 is significant functional redundancy between DNA ligase IIIalpha and DNA ligase I in excision repair.

171 Expression of DNA ligase IIIalpha and the association between MRN and

172 ame is true for a protein complex comprising DNA ligase IIIalpha and the scaffolding protein X-ray re

173 rmed that the expression levels of PARP1 and DNA ligase IIIalpha correlated with the sensitivity to t

174 ike its other nuclear functions, the role of DNA ligase IIIalpha in alternative NHEJ is independent o

175 DNA ligase IIIalpha is a component of an alternative non

176 In addition, DNA ligase IIIalpha is essential for DNA replication in

177 DNA ligase IIIalpha is frequently overexpressed in cance

178 therapy to inhibit FLT3/ITD signaling and/or DNA ligase IIIalpha may lead to repair that reduces repa

179 Thus, the expression levels of PARP1 and DNA ligase IIIalpha serve as biomarkers to identify a su

180 Concomitantly, levels of DNA ligase IIIalpha, a component of ALT NHEJ, are increa

181 es with the steady state levels of PARP1 and DNA ligase IIIalpha, and ALT NHEJ activity.

182 , poly-(ADP-ribose) polymerase 1 (PARP1) and DNA ligase IIIalpha, were increased in the BCR-ABL1-posi

183 ermore, the interaction between PNKP and the DNA ligase IIIalpha-XRCC1 complex significantly increase

184 ular apurinic/apyrimidinic endonuclease, and DNA ligase IIIalpha-XRCC1, performs uracil-initiated bas

185 In contrast, DNA ligase IIIalpha-XRCC1, which completes BER, was appr

186 IIIalpha and the association between MRN and DNA ligase IIIalpha/XRCC1 are altered in cell lines defe

187 MRN interacts with DNA ligase IIIalpha/XRCC1, stimulating intermolecular li

188 en two factors, hMre11/hRad50/Nbs1 (MRN) and DNA ligase IIIalpha/XRCC1, that have been linked with al

189 We find that LIG4, a DNA ligase in DNA double-strand break repair, is a direc

190 system to show increased sensitivity over T4 DNA ligase in the specific detection of a target mRNA.

191 onal interaction between DNA polymerases and DNA ligases in the repair of single- and double-strand D

192 ck-like dGTP insertion opposite T, using BER DNA ligases in vitro.

193 DNA was dependent on expression of the viral DNA ligase, in accord with previous proteomic studies.

194 Here, we show that, although the DNA ligase inhibitor selectively targets mitochondria, c

195 Although a shared feature of DNA ligases is their envelopment of the nicked duplex as

196 cs), XRCC4-like factor (XLF), and XRCC4 (X4)-DNA ligase IV (L4).

197 The XRCC4 (X4)-DNA Ligase IV (LIG4) complex (X4LIG4) executes the final

198 DNA ligase IV (LIG4) is an essential component of the no

199 logous end-joining (NHEJ) DNA repair protein DNA ligase IV (LIG4) lead to immunodeficiency with varyi

200 nduced apoptosis after ionizing radiation or DNA ligase IV (Lig4) loss in the Mre11(ATLD1/ATLD1) nerv

201 Deletion of DNA ligase IV (Lig4), a core component of the NHEJ pathw

202 ical NHEJ (c-NHEJ) components, which include DNA ligase IV (LIG4), and instead arise from alternative

203 reviously, we showed that mice deficient for DNA ligase IV (Lig4), another key NHEJ factor, succumbed

204 ns, including KU70, KU80, ARTEMIS, DNA-PKcs, DNA ligase IV (LIG4), Ataxia telangiectasia mutated (ATM

205 double-strand break repair (DSBR) proteins, DNA Ligase IV (Lig4), Xrcc2, and Brca2, or combined Lig4

206 ) which relies on Ku binding to DNA ends and DNA Ligase IV (Lig4)-mediated ligation.

207 seven genes Ku70, Ku86, DNA-PK(cs), Artemis, DNA Ligase IV (LIGIV), X-ray cross-complementing group 4

208 of nucleases, DNA polymerases, and the XRCC4-DNA ligase IV (X4-LIV) complex in an order influenced by

209 nce of some end joining on only Ku and XRCC4.DNA ligase IV allows us to formulate a physical model th

210 sociates with the core NHEJ components XRCC4-DNA ligase IV and Ku.

211 have evolved mechanisms based on the loss of DNA ligase IV and perhaps other unknown molecules to dis

212 mFRET), we show here that both Ku plus XRCC4:DNA ligase IV are necessary and sufficient to achieve a

213 n donor, it was recently reported that human DNA ligase IV can also utilize NAD+ and, to a lesser ext

214 Thus, we conclude that human DNA ligase IV cannot use either NAD+ or ADP-ribose as ad

215 XLF stimulates the XRCC4/DNA ligase IV complex by an unknown mechanism.

216 te that APLF promotes the retention of XRCC4/DNA ligase IV complex in chromatin, suggesting that PARP

217 se mu and lambda to add nucleotides; and the DNA ligase IV complex to ligate the ends with the additi

218 Surprisingly, Mre11 and DNA ligase IV degradation do not appear to be significan

219 protein kinase catalytic subunit, and XRCC4-DNA ligase IV do not modulate PALF nuclease activity on

220 lled products can be subsequently ligated by DNA Ligase IV during Nonhomologous end-joining.

221 the precise visualization of XRCC4, XLF, and DNA ligase IV filaments adjacent to DSBs, which bridge t

222 structurally related proteins important for DNA Ligase IV function.

223 over, we find that ligation by de-adenylated DNA ligase IV is dependent upon ATP not NAD+ or ADP-ribo

224 1, B1, D, and B2, respectively) only affects DNA ligase IV levels.

225 e-molecule FRET analysis of the Ku/XRCC4/XLF/DNA ligase IV NHEJ ligation complex, that end-to-end syn

226 3' overhanging nucleotides and permit XRCC4-DNA ligase IV to complete the joining process in a manne

227 XRCC4 forms a tight complex with DNA Ligase IV while XLF interacts directly with XRCC4.

228 t disruption of DSB repair factors (Rad51 or DNA ligase IV) or DSB sensing factors (ATRIP or MDC1) re

229 In flies deficient in lig4 (DNA ligase IV), a component of the major NHEJ pathway, t

230 Here, we report that human DNA ligase IV, a key enzyme in DNA double-strand break (

231 mote HDR at the expense of NHEJ, we targeted DNA ligase IV, a key enzyme in the NHEJ pathway, using t

232 espectively) cause the degradation of Mre11, DNA ligase IV, and p53, infection with Ad3, Ad7, Ad9, an

233 se by facilitating the degradation of Mre11, DNA ligase IV, and p53.

234 ed end joining assay that includes Ku, XRCC4-DNA ligase IV, and PALF, PALF is able to resect 3' overh

235 athway that operates in the absence of XRCC4/DNA ligase IV, and that the requirement for PARP-3 and A

236 by RNA interference diminished expression of DNA ligase IV, Artemis, and Ku80 components in DNA-depen

237 largely resemble those from patients lacking DNA ligase IV, Artemis, or ATM, suggesting that these fa

238 We found that insertions required Ku80 and DNA ligase IV, as well as polymerase IV.

239 does not enhance ligation by pre-adenylated DNA ligase IV, indicating that this co-factor is not uti

240 s been known that Ku-dependent NHEJ requires DNA ligase IV, it is unclear which DNA ligase(s) is requ

241 recipients deficient in the NHEJ component, DNA ligase IV, the majority of products arise by HR with

242 we found HF-NHEJ to be strictly dependent on DNA Ligase IV, XRCC4 and XLF, members of the canonical b

243 induced the association of these factors in DNA ligase IV-deficient cells.

244 B) repair that is more active when the major DNA ligase IV-dependent pathway is defective.

245 w that cohered sister telomeres are fused by DNA ligase IV-mediated nonhomologous end joining.

246 DP-ribose can be used as co-factors by human DNA ligase IV.

247 to create substrates ultimately ligatable by DNA Ligase IV.

248 ze as heterotypic filaments independently of DNA Ligase IV.

249 J is circumvented by overexpression of XRCC4/DNA ligase IV.

250 n, as well as targeting the effector protein DNA ligase IV.

251 Thr181 to trigger its dissociation from the DNA ligase IV/XRCC4 complex, and promotes its interactio

252 omise the end joining by DNA ligase I or the DNA ligase IV/XRCC4 complex.

253 cs autophosphorylation, and it also inhibits DNA ligase IV/XRCC4-mediated end rejoining.

254 plex of NHEJ factors that includes a ligase (DNA Ligase IV; L4) that relies on juxtaposition of 3 hyd

255 DNA ligases join 3'-OH and 5'-PO(4) ends via a series of

256 autonomous enzymatic modules: ATP-dependent DNA ligase (LIG), DNA/RNA polymerase (POL), and 3' phosp

257 cribe a complete NHEJ complex, consisting of DNA ligase (Lig), polymerase (Pol), phosphoesterase (PE)

258 Among the mammalian genes encoding DNA ligases (LIG), the LIG3 gene is unique in that it en

259 d by deletion of either of the two remaining DNA ligases (Lig1 and nuclear Lig3) in Lig4(-/-) cells.

260 NAD(+)-dependent DNA ligases (LigA) are ubiquitous in bacteria, where the

261 r in mammalian cells involves three distinct DNA ligases: ligase I (Lig1), ligase III (Lig3) and liga

262 Nevertheless, PBCV-1 DNA ligase ligated all sequences tested with 10-fold les

263 PBCV-1 DNA ligase ligated ssDNA splinted by RNA with kcat appro

264 um smegmatis that requires the ATP-dependent DNA ligase LigC1 and the POL domain of LigD.

265 nd binding protein Ku and the polyfunctional DNA ligase LigD.

266 i that are critical for ligation by the NHEJ DNA ligase, LigIV.

267 ssential step in most repair pathways is the DNA ligase-mediated rejoining of single- and double-stra

268 out a synthetic lethal screen with cdc9-p, a DNA ligase mutation with two substitutions (F43A/F44A) i

269 es the Vsr endonuclease, DNA polymerase I, a DNA ligase, MutS, and MutL to function at peak efficienc

270 Bacterial DNA ligases, NAD(+)-dependent enzymes, are distinct from

271 ndicating that the rate-limiting step for T4 DNA ligase nick sealing is not a chemical step but rathe

272 of two fluorescently labeled DNA with the T4 DNA ligase on the single-molecule level.

273 SplintR Ligase is 100X faster than either T4 DNA Ligase or T4 RNA Ligase 2 for RNA splinted DNA ligat

274 3'-PO4 ends that cannot be sealed by classic DNA ligases or extended by DNA polymerases.

275 ation of RNA-splinted DNA by Chlorella virus DNA ligase (PBCV-1 DNA ligase).

276 3 gene is unique in that it encodes multiple DNA ligase polypeptides with different cellular function

277 y classic ATP-dependent and NAD(+)-dependent DNA ligases, prevents template-independent nucleotide ad

278 nM at 25 degrees C under conditions where T4 DNA ligase produced only 5'-adenylylated DNA with a 20-f

279 n of Ku80 and a molecular mechanism by which DNA ligase proficient complexes are assembled during NHE

280 We show that the T4 DNA ligase repairs sticky ends more efficiently than blu

281 protein that recruits the DNA polymerase and DNA ligase required for filling and sealing the damaged

282 requires DNA ligase IV, it is unclear which DNA ligase(s) is required for Ku-independent MHEJ.

283 DNA ligases seal 5'-PO4 and 3'-OH polynucleotide ends vi

284 ment synthesis that was cleaved by Fen1, and DNA ligase sealed the nick for complete repair.

285 tection method that utilizes Chlorella virus DNA ligase (SplintR((R)) Ligase).

286 findings led to a series of experiments with DNA ligase that reveal, contrary to expectation based up

287 e mitochondria or expressing Chlorella virus DNA ligase, the minimal eukaryal nick-sealing enzyme, or

288 Unlike reactions that use T4 DNA ligase, this protocol does not require synthesis of

289 city of a DNA-dependent RNA polymerase and a DNA ligase to act as RNA-dependent RNA polymerase and RN

290 (Fen1) and the resultant nick was ligated by DNA ligase to form a mature lagging strand.

291 ely functionalized nucleic acids by using T4 DNA ligase to mediate the DNA-templated polymerization o

292 ng a DNA polymerase, an RNA polymerase and a DNA ligase, to use Fe2+ in place of Mg2+ as a cofactor d

293 While it has been shown that eukaryotic DNA ligases utilize ATP as the adenylation donor, it was

294 utants of KU70, KU80, and the plant-specific DNA Ligase VI (LIG6) showed increased stable transformat

295 Here, we report the characterization of DNA LIGASE VI, which is only found in plant species.

296 with the DNA-binding domain of Ligase IV, a DNA Ligase which plays essential roles in DNA repair and

297 igation strategy based on USER Enzyme and T4 DNA ligase, which allows the simultaneous and seamless a

298 Mammalian cells have three ATP-dependent DNA ligases, which are required for DNA replication and

299 Humans have three genes encoding DNA ligases with conserved structural features and activ

300 he cyclase pathway resemble those of RNA and DNA ligases, with the key distinction being that ligases

301 t the modification site that is sealed by T4-DNA ligase, yielding a product strand missing the modifi