ライフサイエンスコーパス: DNA nick

1 diate featuring a U*G mismatch across from a DNA nick.

2 a putative mimetic of the 5'-phosphate at a DNA nick.

3 flap DNA substrate and processing it into a DNA nick.

4 nicked DNA-adenylate, but not to a standard DNA nick.

5 inantly ligase-adenylate, binds tightly to a DNA nick.

6 denylate but has low affinity for a standard DNA nick.

7 tion and repair by catalyzing the joining of DNA nicks.

8 ome core particles (NCP) and NCPs containing DNA nicks.

9 cessed 3' end (3' ss/dsDNA junctions) and at DNA nicks.

10 ave a feeble capacity to seal 3'-OH/5'-PO(4) DNA nicks.

11 at tunes the enzyme to release pro-mutagenic DNA nicks.

12 lex RNA or an RNA:DNA hybrid but cannot seal DNA nicks.

13 DNA hybrid, but it cannot seal 3'-OH/5'-PO4 DNA nicks.

14 viability assessed by morphology and reduced DNA nicking.

15 ctive nucleotide incorporation indicative of DNA nicking.

16 ion-induced acidosis, loss of viability, and DNA nicking.

17 ilizes the ribonucleoprotein-DNA complex via DNA nicking.

18 cally in apoptotic cells, around the time of DNA nicking.

19 inhibiting religation and to a lesser extent DNA nicking.

20 ndensation and collapse, and single-stranded DNA nicking.

21 ath pathway characterized by single-stranded DNA nicking.

22 DNA nicks 3' of the (GAA)(100) repeat led to a smaller b

23 We found that DNA nicks 5' of the (GAA)(100) run led to a dramatic inc

24 iments conducted to demonstrate the observed DNA nicking activity is inherent, rather than a co-purif

25 The DNA nicking activity of the wild-type enzyme is still ac

26 Furthermore, we show that the DNA nicking activity of TOP1 is a prerequisite for robus

27 tail or head-to-tail repeat only supported a DNA nicking activity.

28 ge agent, displaying significant supercoiled DNA-nicking activity at concentrations as low as 1 micro

29 Both LigD proteins seal DNA nicks, albeit inefficiently.

30 The cloning and expression of the CviPII DNA nicking and modification system encoded by chlorella

31 ed magnetic tweezers to directly measure the DNA nicking and religation activities of RepC, the repli

32 esidues (Y18,19,26,27) that are required for DNA nicking and religation were displaced up to 14 A out

33 onserved tyrosine, Tyr127, are essential for DNA nicking and the formation of a covalent intermediate

34 y arise from DNA regions expected to undergo DNA nicking and/or double-strand breaks.

35 Metnase possesses a unique DNA nicking and/or endonuclease activity that mediates c

36 l change within the nucleosome to facilitate DNA nicking and/or fragmentation.

37 Exo1 processing at DNA nicks and double-strand breaks creates long stretche

38 ath pathway characterized by single-stranded DNA nicks and other features of apoptosis.

39 SR, and we propose that MMR proteins convert DNA nicks and point mutations into dsDNA breaks for both

40 hibition greatly increases PARP1 affinity to DNA nicks and undamaged NCP, implicating a mechanism whe

41 e findings, purified CdtB(H154A) lacked both DNA-nicking and cell cycle arrest activities.

42 we developed two biochemical methods; i.e., DNA-nicking and DNA-gyrase methods to examine whether ce

43 positioning is unaffected by the presence of DNA nicks, and can occur on closed-circular DNAs in the

44 med during the cleavage reaction: open ends, DNA nicks, and hairpin ends.

45 We further show that nontemplate strand DNA nicks are powerful initiators of R-loop formation, i

46 to discrete nucleosomal fragments, although DNA nicks are readily discernible by terminal deoxynucle

47 DNA nicks are the most common form of DNA damage, and if

48 The reducing power and DNA nicking assay on the methanolic extracts suggested h

49 DNA damage by 3-NPA was elucidated by pBR322 DNA nicking assay.

50 The appearance of DNA nicked at both signals is stimulated by more than an

51 d us to study the consequences of inflicting DNA nicks at EcoRI sites in vivo.

52 on the opposite strand from the Top1-induced DNA nicks at ribonucleotide sites.

53 lity group AT-hook 2 protein (HMGA2) induces DNA nicks at the transcription start site, which are req

54 se activity of topoisomerase I (Top1) causes DNA nicks bearing 2',3'-cyclic phosphates at ribonucleot

55 Hyper-rec mutants, in which DNA nicks become detectable, are synthetic-lethal with r

56 e catalytic core of ligase III is the second DNA nick-binding module.

57 s in single-strand rather than double-strand DNA nicks/breaks.

58 DNA nicking by Can1 is predicted to slow down viral repl

59 single-stranded DNA-assisted double-stranded DNA nicking by DNAzymes (DANDA), expanded the substrate

60 nucleic acid (PNA)-assisted double-stranded DNA nicking by DNAzymes (PANDA) as the first example to

61 As DNA nicking by MutLgamma depends on its co-factors, the

62 g of 5'-phosphate and 3'-hydroxyl termini at DNA nicks by means of a series of three nucleotidyl tran

63 Replication fork collision with a DNA nick can generate a one-ended break, fostering genom

64 rvations suggest that an increased number of DNA nicks can overwhelm the repair capacity of DNA ligas

65 e, a PleI isoschizomer, does not exhibit any DNA nicking/cleavage activity, being completely blocked

66 In addition to its classic DNA nicking-closing functions, Top1 plays critical noncl

67 mediates, including single-nucleotide-gapped DNA, nicked DNA, and nicked DNA with various lengths of

68 riety of substrates including partial duplex DNA, nicked DNA, forked DNA structures, blunt duplex DNA

69 atalyses four phosphoryl transfer reactions (DNA nicking, DNA hairpin formation, hairpin resolution a

70 nt, high-affinity binding to single-stranded DNA, nicked double-stranded DNA, and RNA.

71 s suggests that, although DNA ligase I seals DNA nicks during replication, repair, and recombination,

72 asing the lifetime of replication-associated DNA nicks, either by reducing or delaying Cdc9 ligase ac

73 Apoptotic cell death was characterized by DNA nick end and single-stranded DNA labeling.

74 d apoptosis by terminal transferase-mediated DNA nick end labeling assay and measured expression of a

75 rminal deoxynucleotidyl transferase-mediated DNA nick end-labeling-positive neurons contained p18 or

76 TNF-alpha-induced apoptosis was detected by DNA nick-end labeling and by measuring histone associate

77 own by a decrease in cell viability, in situ DNA nick-end labeling, and internucleosomal DNA fragment

78 had a marked apoptotic effect documented by DNA nick-end labeling, or DNA agarose gels in xenografts

79 By DNA nick-end labeling, the number of dying putaminal cel

80 t had been appropriately preserved to permit DNA nick-end labeling.

81 aluated 20-22 h after sepsis by annexin V or DNA nick-end labeling.

82 ronchoalveolar lavage (BAL), histopathology, DNA nick-end-labeling assays, and electron microscopy.

83 is includes: (1) labeling of cell nuclei for DNA nicked ends; (2) morphological changes in ultrastruc

84 Gamma is a strand-specific and site-specific DNA nicking enzyme (YCG downward arrowGT or AC upward ar

85 dependent cleavage of molecular beacons by a DNA nicking enzyme, through which one target DNA can ope

86 e large subunits behave as sequence-specific DNA nicking enzymes and only nick the bottom strand of t

87 These DNA-nicking enzymes could prove useful for investigation

88 4 bp downstream from the Top1 site enhances DNA nicking especially when the 3' end of the TFO is pro

89 out in which peroxynitrite-mediated plasmid DNA nick formation in the presence or absence of organos

90 promotes meiotic crossing over by protecting DNA nicks from ligation.

91 n toolkit, enabling off-target discovery for DNA nicking gene editors such as prime editors.

92 processing and that LigI engages PCNA at the DNA nick generated by FEN1 and Pol delta.

93 cation relies on DNA ligase 1 (LIG1) to seal DNA nicks generated during Okazaki Fragment Maturation (

94 nt complex formation, but not resealing of a DNA nick in a preformed covalent complex.

95 ains of Cas9, to create a guide RNA-directed DNA nick in the context of an in vitro-assembled CRISPR-

96 s observation, coupled with the detection of DNA nicking in cells subjected to metabolic inhibition,

97 ed only GzmA to reconstitute single-stranded DNA nicking in isolated nuclei.

98 ivation and SET cleavage at N175, triggering DNA nicking in wild-type, but not AEP null, mice.

99 t the ligase IIIalpha-XRCC1 complex binds to DNA nicks in nucleosomes only when they are exposed by p

100 To investigate the role of DNA nicks in this process, we utilized a CRISPR-Cas9 nic

101 sulting in the conversion of a proportion of DNA nicks into DNA lesions that require recombination fo

102 DNA nick sensor, but it is not required for DNA nick joining activity in vitro.

103 nc finger (ZnF) that increases the extent of DNA nick joining and intermolecular DNA ligation, yet th

104 ained in part by a shift toward a processive DNA nicking mechanism, which leads to a higher frequency

105 Because DNA nicks occur on both newly synthesized leading and la

106 DNA nicking occurred independently of caspase activation

107 io are demonstrated via the marked effect of DNA nicking on histone eviction that underscores the pow

108 DNA nicks, one of the most frequent DNA lesions in cells

109 repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-d

110 The target of APTX is 5'-adenylates at DNA nicks or breaks that result from abortive DNA ligati

111 Here we demonstrate that DNA nicks or double-strand breaks (DSBs) targeted by CRI

112 ideal either for ligation (in case of a DNA-DNA nick) or for subsequent engagement by FEN1 (in case

113 nucleosomes containing discretely positioned DNA nicks, our evidence indicates that the ligase IIIalp

114 d by a pathway involving templated repair at DNA nicks rather than double-strand breaks.

115 ejections, which proceeded stepwise between DNA nicks, reaching a translocation speed of 75 kbp/s or

116 are critical for both nucleotide binding and DNA nick recognition.

117 c roles, as it contains both single-stranded DNA-nicking relaxase and ATP-dependent helicase domains

118 Mpa Lig has limited DNA nick-sealing activity but is efficient in ligating n

119 This small domain has been described as a DNA nick sensor, but it is not required for DNA nick joi

120 ific interaction between condensin I and the DNA nick-sensor poly(ADP-ribose) polymerase 1 (PARP-1).

121 polymerase the novel function of a molecular DNA nick-sensor, and that the DNA ligase can inhibit act

122 to the recruitment of PcrA-like helicases to DNA-nick sites and the processive translocation of the P

123 s DNA elements and strongly suggest that the DNA nicking step of V(D)J recombination can be rate limi

124 Recent findings suggest that DNA nicks stimulate homologous recombination by being co

125 tide nucleic acid (PNA)-directed design of a DNA-nicking system that enables selective and quantitati

126 uclease(12) (APE1), creating single-stranded DNA nicks that can be converted to DNA double strand bre

127 ndow of time for MMR determined by transient DNA nicks that direct the Mlh1-Pms1 in a strand-specific

128 gand-dependent enhancer activation, based on DNA nicking to relieve torsional stress from eRNA synthe

129 The relative number of DNA nicks to phosphodiester bonds in a circular plasmid

130 the MMR machinery is important in processing DNA nicks to produce double-stranded breaks, particularl

131 lated, topo I, and increased topo I-mediated DNA nicking under conditions of oxidative stress.

132 lation sites separating the mismatch and the DNA nick used to direct repair, from 10 to 808 base pair

133 a direct comparison of the thermodynamics of DNA nicks versus DNA gaps has not been performed.

134 that Ca(2+) and Mg(2+) promote single-strand DNA nicks, whereas Mn(2+) promotes double-strand DNA bre

135 t catalyzes phosphodiester bond formation at DNA nicks with 3' hydroxyl and 5' phosphate termini.