ライフサイエンスコーパス: DNA polymerase I

1 palm domains of phosphodiesterases, such as DNA polymerase I.

2 Arg(841) present in the fingers subdomain of DNA polymerase I.

3 were extended with dATP by Escherichia coli DNA polymerase I.

4 deficient large fragment of Escherichia coli DNA polymerase I.

5 ase-free Klenow fragment of Escherichia coli DNA polymerase I.

6 polymerase beta or Klenow fragment of E.coli DNA polymerase I.

7 eficient Klenow fragment of Escherichia coli DNA polymerase I.

8 ly in a crystal of the thermostable Bacillus DNA polymerase I.

9 s by the Klenow fragment of Escherichia coli DNA polymerase I.

10 th the equivalent region of Escherichia coli DNA polymerase I.

11 s, beta interacts with MutS, DNA ligase, and DNA polymerase I.

12 the proofreading domain of Escherichia coli DNA polymerase I.

13 (exo(-)) Klenow fragment of Escherichia coli DNA polymerase I.

14 exo-nuclease III and the Klenow fragment of DNA polymerase I.

15 atalytic site (motif A) of Thermus aquaticus DNA polymerase I.

16 oofreading by the Klenow fragment of E. coli DNA polymerase I.

17 reverse transcriptase, T7 DNA polymerase, or DNA polymerase I.

18 not the Klenow fragment of Escherichia coli DNA polymerase I.

19 -3' exonuclease function of Escherichia coli DNA polymerase I.

20 e to restriction endonucleases, and notably, DNA polymerase I.

21 ge proteolytic fragment (Klenow fragment) of DNA polymerase I.

22 now fragment (KF exo-) from Escherichia coli DNA polymerase I.

23 s and also by the Klenow fragment of E. coli DNA polymerase I.

24 prior work on the mechanism and dynamics of DNA polymerase I.

25 gue, the Klenow fragment of Escherichia coli DNA polymerase I.

26 ly from the closely related Escherichia coli DNA polymerase I.

27 taricus Dpo4 and Bacillus stearothermophilus DNA polymerase I.

28 of palm domain containing proteins, such as DNA polymerase I.

29 ase-free Klenow fragment of Escherichia coli DNA polymerase I.

30 Herpes simplex virus DNA polymerase is a heterodimer composed of a catalytic

31 Herpes simplex virus DNA polymerase is a heterodimer composed of UL30, a cata

32 Translesion synthesis (TLS) by Y-family DNA polymerases is a chief mechanism of DNA damage toler

33 FEN1 or the 5'-nuclease domains of bacterial DNA polymerases is a double-flap structure containing a

34 Proofreading by replicative DNA polymerases is a fundamental mechanism ensuring DNA

35 epair pathway requires the Vsr endonuclease, DNA polymerase I, a DNA ligase, MutS, and MutL to functi

36 ue of the small fragment of Escherichia coli DNA polymerase I, a FEN with which it shares 66% similar

37 he interesting question of how a replicative DNA polymerase is able to recognize templates of diverse

38 elity of DNA replication by bacteriophage T4 DNA polymerase is achieved in a multiplicative process:

39 e critical viral replication gene (the viral DNA polymerase) is activated by E2F.

40 proposed function of Pol zeta as an extender DNA polymerase is also required for ICL repair.

41 Therminator DNA polymerase is an efficient DNA-dependent TNA polymer

42 s less topo I was recruited, suggesting that DNA polymerase is an important modulator of the binding

43 DNA polymerase, a variant of the 9 degrees N DNA polymerase, is an efficient DNA-directed threosyl nu

44 DNA synthesis (TLS) mediated by low-fidelity DNA polymerases is an essential cellular mechanism for b

45 iota (hPoliota), a member of the Y family of DNA polymerases, is an exception to these rules.

46 The Klenow fragment of DNA polymerase I (an A-family polymerase) can efficientl

47 e interaction between the Klenow fragment of DNA polymerase I and a series of defined oligonucleotide

48 ase active site motifs with Escherichia coli DNA polymerase I and bacteriophage T7 DNA polymerase.

49 the single strand break by endonuclease IV, DNA polymerase I and DNA ligase occurred and was not gre

50 nzyme process requires six proteins: UvrA-D, DNA polymerase I and DNA ligase.

51 nd break can be repaired by Escherichia coli DNA polymerase I and E. coli DNA ligase alone, though le

52 osite a single strand break, endonuclease IV DNA polymerase I and Escherichia coli DNA ligase are req

53 ve site and in different segments of E. coli DNA polymerase I and have determined the effects of thes

54 A-binding proteins, the Klenow fragment from DNA polymerase I and Klenow exonuclease minus (which has

55 E. coli DNA polymerase I and Mycobacterium DinB1 extend the DNAp

56 amers did not inhibit the Klenow fragment of DNA polymerase I and only had a minor effect on RB69 DNA

57 e interaction between the Klenow fragment of DNA polymerase I and synthetic DNA primer-templates cont

58 sive sequence homology with Escherichia coli DNA polymerase I and T7 DNA polymerase, it contains uniq

59 of nucleotide repeats during replication by DNA polymerase I and that this action provides insight i

60 the Klenow fragment of the Escherichia coli DNA polymerase I and the Bacillus stearothermophilus pol

61 instead required polA and polC, which encode DNA polymerase I and the second DNA polymerase III enzym

62 combine single-molecule FRET with the use of DNA polymerase I and various fidelity mutants to highlig

63 ere also studied with the Klenow fragment of DNA polymerase I and with T4 DNA polymerase.

64 onucleases, especially those associated with DNA Polymerases I and III, affect inheritance of the sil

65 en verified by primer extension studies with DNA polymerases I and IV from E. coli.

66 riophage T4 DNA polymerase, Escherichia coli DNA polymerase I, and E.coli exonuclease III.

67 ists of UvrA, UvrB, UvrC, the UvrD helicase, DNA polymerase I, and ligase.

68 olymerization rate of the Klenow fragment of DNA polymerase I, and we demonstrate its combination wit

69 good efficiency with the Klenow fragment of DNA polymerase I, and we identify thermostable enzymes t

70 labeled using digoxigenin-11-dUTP and Klenow DNA polymerase-I, and detected using fluorescein isothio

71 I since very-long patch BER was inhibited by DNA polymerase I antibody and addition of excess DNA pol

72 m of the Klenow fragment of Escherichia coli DNA polymerase I as well as DNA polymerase of Thermus aq

73 small amounts of template by RCA using phi29 DNA polymerase is "background" DNA synthesis that usuall

74 tolerance to mutation in the active sites of DNA polymerases is being exploited to engineer polymeras

75 e or the Klenow fragment of Escherichia coli DNA polymerase I, both nucleotides failed to substitute

76 ort the crystal structure of a high fidelity DNA polymerase I bound to DNA primer-template caught in

77 bases can migrate through the run while the DNA polymerase is bound to the template-primer, or the D

78 modification does not affect the activity of DNA polymerase I, but four vinylphosphonate linkages in

79 In prokaryotes, this process is completed by DNA polymerase I by means of strand displacement DNA syn

80 The Klenow fragment of Escherichia coli DNA polymerase I catalyzes template-directed synthesis o

81 The addition of exogenous DNA polymerase I caused modest inhibition of BER, which

82 Accurate copying of the genome by DNA polymerases is challenging due in part to the contin

83 of DNA polymerse I in Escherichia coli, the DNA polymerase I class of enzymes has served as the prot

84 are presented for a Thermus aquaticus (Taq) DNA polymerase I complex (consisting of the protein, the

85 The human cytomegalovirus DNA polymerase is composed of a catalytic subunit, UL54,

86 DinB, a Y-family DNA polymerase, is conserved among all domains of life;

87 Bypass of DNA lesions by translesion DNA polymerases is conserved in bacteria, yeast, and mam

88 We show that PolC is highly dynamic: this DNA polymerase is constantly recruited to and released f

89 nd, the highly homologous Klenow fragment of DNA polymerase I containing an engineered gp5 thioredoxi

90 ce present in the Mycobacterium tuberculosis DNA polymerase I corresponds to a hinge region in the fi

91 greatly stimulated when DNA synthesis by T7 DNA polymerase is coupled to DNA unwinding.

92 upled with the conformational transitions in DNA polymerases is critical for maintaining the fidelity

93 Fidelity of DNA synthesis, catalyzed by DNA polymerases, is critical for the maintenance of the

94 Klenow fragment (exo(-)) of Escherichia coli DNA polymerase I demonstrate that bypass of LdG modifica

95 ons with the 3'-5' exo(-) Klenow fragment of DNA polymerase I demonstrate the usefulness of our proto

96 gorithms and reanalyzed experimental data of DNA polymerase I diffusing in live Escherichia coli.

97 Telomerase is the cellular RNA-dependent DNA polymerase (i.e. reverse transcriptase) that uses an

98 We found that a mutated form of KlenTaq DNA polymerase, i.e., KTqM747K, catalyzed O(6)-BnG adduc

99 uctures provide the first direct evidence in DNA polymerase I enzymes of a large conformational chang

100 ha (pol alpha) and Klenow fragment (exo-) of DNA polymerase I (Escherichia coli).

101 DNA polymerase alpha and Klenow fragment of DNA polymerase I (Escherichia coli).

102 e chemical step in the catalytic reaction of DNA polymerases is essential for elucidating the molecul

103 catalytic subunit of the eukaryotic B-family DNA polymerases is essential for the formation of active

104 n of UL42 and BMRF1 with their corresponding DNA polymerases is essential for viral DNA replication a

105 ass experiments performed in vitro utilizing DNA polymerase I exo-.

106 ther the 5' --> 3' exonuclease activities of DNA polymerase I, exonuclease VII, or RecJ.

107 cluding exonucleases I-IX (including the two DNA polymerase I exonucleases), RecJ exonuclease, SbcCD

108 ear antigen (PCNA), the auxiliary factor for DNA polymerase , is expressed zygotically in the develop

109 not present in other members of the E. coli DNA polymerase I family.

110 loop is not present in other members of the DNA polymerase I family.

111 of dATP and dGTP analogues to determine how DNA polymerase I from Bacillus stearothermophilus (BF),

112 tensive studies using the Klenow fragment of DNA polymerase I from E. coli (Kf) and found to be recog

113 tic experiments using the Klenow fragment of DNA polymerase I from E. coli suggest that a low dA misi

114 substrate by the Klenow fragment (exo(-)) of DNA polymerase I from E. coli.

115 The Klenow fragment of DNA polymerase I from Escherichia coli accepted Fapy.dGT

116 eolytic active site of the large fragment of DNA polymerase I from Escherichia coli have been elucida

117 reaction catalyzed by the Klenow fragment of DNA polymerase I from Escherichia coli.

118 c active site of the Klenow fragment (KF) of DNA polymerase I from Escherichia coli.

119 eal overdamped, coupled domain motion within DNA polymerase I from Thermus aquaticus (Taq polymerase)

120 The DNA polymerase I from Thermus aquaticus (Taq polymerase)

121 substrate recognition in the active site of DNA polymerase I from Thermus aquaticus (Taq) and select

122 designed based on two unique features of the DNA polymerase I gene (polA).

123 and has low homology with similar regions of DNA polymerase I gene from known microorganisms.

124 eaction (PCR) amplification of a T. pallidum DNA polymerase I gene.

125 a 260 bp region of the small fragment of the DNA polymerase I gene.

126 e binding established previously for several DNA polymerases is generally extended to all DNA polymer

127 onuclease activity of the Klenow fragment of DNA polymerase I has been investigated with a combinatio

128 nd cannot grow at moderate temperatures, its DNA polymerase I has significant activity at 20-37 degre

129 dPTP) by exonuclease-free Klenow fragment of DNA polymerase I have been determined.

130 at positions 705 and 882 of Escherichia coli DNA polymerase I have been well established.

131 The Rev1 DNA polymerase is highly specialized for the incorporati

132 G), when produced in situ or incorporated by DNA polymerases, is highly mutagenic due to mispairing w

133 ulgidus and Methanococcus jannaschii and the DNA polymerase I homologues from Thermus aquaticus and T

134 The Klenow fragment of Escherichia coli DNA polymerase I houses catalytic centers for both polym

135 sly, it has been shown that Escherichia coli DNA polymerases I, II, and III are incapable of bypassin

136 Escherichia coli DNA polymerases I, II, IV and V (UmuC) interact with bet

137 ds developed in this study are recognized by DNA polymerases is important in view of the future selec

138 sing the exonuclease-free Klenow fragment of DNA polymerase I in a primer extension reaction.

139 propose a new in vivo function for wild-type DNA polymerase I in chromosome termination at site(s) no

140 t the crystal structure of Thermus aquaticus DNA polymerase I in complex with an inhibitory Fab, TP7,

141 the closed, open, and ajar conformations of DNA polymerase I in the binary (enzyme:DNA) state to bet

142 RNase D and the 3'-5' exonuclease domain of DNA polymerase I in the Werner syndrome gene product.

143 that the efficiency of primer processing by DNA polymerase I in vitro is specifically affected by th

144 The Klenow exo- fragment of Escherichia coli DNA polymerase I incorporated 2'-deoxyadenosine (dA) six

145 Taq, the large fragment of Thermus aquaticus DNA polymerase I, incorporates a nucleotide opposite an

146 The vaccinia virus DNA polymerase is inherently distributive but acquires p

147 Although the vaccinia virus DNA polymerase is inherently distributive, a highly proc

148 Archaeal family-D DNA polymerase is inhibited by the presence of uracil in

149 forks or suppress futile origin firing when DNA polymerase is inhibited, leading to incomplete genom

150 ts demonstrate that the COOH terminus of the DNA polymerase is inserted into the subunit interface of

151 g single molecules of the Klenow fragment of DNA polymerase I into electronic nanocircuits allowed el

152 a also suggest that an aphidicolin-sensitive DNA polymerase is involved in the excision step of the n

153 tein, a eukaryotic member of the Y family of DNA polymerases, is involved in the tolerance of DNA dam

154 e Stoffel fragment (SF) of Thermus aquaticus DNA polymerase I is displayed on a filamentous phage by

155 Additionally, we identify that DNA polymerase I is important for both primed and naive

156 the thermodynamics of substrate selection by DNA polymerase I is important for characterizing the bal

157 DNA polymerase I is proposed to fill DNA gaps during spa

158 to modulate DNA strand slippage synthesis by DNA polymerase I, is a wedge-shaped spirocyclic molecule

159 and previous observations that mitochondrial DNA polymerase is itself a reverse transcriptase, we pro

160 The Klenow fragment of Escherichia coli DNA polymerase I (KF) achieves this through a series of

161 uses the Klenow fragment of Escherichia coli DNA polymerase I (KF) and fluorescently labeled primer/t

162 with the Klenow fragment of Escherichia coli DNA polymerase I (KF) as a function of the concentration

163 use the Klenow fragment of Escherichia coli DNA polymerase I (KF) as a model proofreading polymerase

164 and the Klenow fragment of Escherichia coli DNA polymerase I (KF) incorporate all four nucleotide an

165 nt burst synthesis rate for Escherichia coli DNA Polymerase I (KF) was found to be an order of magnit

166 e and replicated with the Klenow fragment of DNA polymerase I (Kf).

167 erase (T7DNAP) and by the Klenow fragment of DNA polymerase I (KF).

168 sence of Klenow fragment of Escherichia coli DNA polymerase I (Kfexo(-)) and DNA polymerase beta (pol

169 mplates for a model enzyme, Escherichia coli DNA polymerase I Klenow fragment (exo-).

170 Here, individual DNA polymerase I Klenow fragment (KF) molecules were tet

171 A substrate between the pol and exo sites of DNA polymerase I Klenow fragment (KF).

172 n by examining the structure and dynamics of DNA polymerase I Klenow Fragment (Pol) substrates both a

173 However, DNA polymerase I Klenow fragment and avian myeloblastosi

174 E. coli DNA polymerase I Klenow fragment could employ 1-me-dATP

175 In the same constructs, Escherichia coli DNA polymerase I Klenow Fragment exo(-) is much less eff

176 a much better substrate for the model enzyme DNA polymerase I Klenow fragment lacking proofreading ac

177 tilizing the Escherichia coli UvrD helicase, DNA polymerase I Klenow fragment, two accessory proteins

178 an indirect assay employing Escherichia coli DNA polymerase I (Klenow enzyme) and poly(dT) template.

179 he dissociation constants for the binding of DNA polymerase I (Klenow fragment) (KF) to the primer-te

180 r extension by exonuclease-deficient E. coli DNA polymerase I (Klenow fragment) (KF) when (+)-trans-

181 e insertion by exonuclease-deficient E. coli DNA polymerase I (Klenow fragment) across from either th

182 ite-specific mutagenesis of Escherichia coli DNA polymerase I (Klenow fragment) and atomic substituti

183 of an exonuclease-deficient Escherichia coli DNA polymerase I (Klenow fragment) bound to primer-templ

184 rboxylate ligands, Asp(705) and Asp(882), of DNA polymerase I (Klenow fragment) in the early prechemi

185 mechanism of bypass by the Escherichia coli DNA polymerase I (Klenow fragment) in the presence of th

186 In particular, Arg668 of Escherichia coli DNA polymerase I (Klenow fragment) makes a critical cont

187 fer, we observed individual Escherichia coli DNA polymerase I (Klenow fragment) molecules performing

188 approach to monitor the movement of E. coli DNA polymerase I (Klenow fragment) on a DNA template dur

189 ts show that the binding of Escherichia coli DNA polymerase I (Klenow fragment) to a primer-template

190 of an exonuclease-deficient Escherichia coli DNA polymerase I (Klenow fragment) to DNA primer-templat

191 tudy the affinity and kinetics of binding of DNA Polymerase I (Klenow fragment) to DNA.

192 he mutant sites of duplex DNA was induced by DNA polymerase I (Klenow fragment) to preserve Watson-Cr

193 hree active-site mutants of Escherichia coli DNA polymerase I (Klenow fragment) were used to study DN

194 nsition that occurs when a binary complex of DNA polymerase I (Klenow fragment) with a primer-templat

195 lymerase ternary complex of Escherichia coli DNA polymerase I (Klenow fragment), containing the templ

196 dG adducts, positioned in the active site of DNA polymerase I (Klenow fragment), serve as donor fluor

197 The principle is based on DNA polymerase I (Klenow fragment)-induced coupling of t

198 n the structure of the exonuclease-deficient DNA polymerase I (Klenow fragment).

199 process were studied using Escherichia coli DNA polymerase I (Klenow fragment, 3'-exonuclease defici

200 mer extension reactions were performed using DNA polymerase I, Klenow fragment exo-.

201 s of the large fragment of Thermus aquaticus DNA polymerase I (Klentaq1) with a primer/template DNA a

202 Rev1's noncatalytic role in recruiting other DNA polymerases is known to be important for TLS.

203 The catalytic reaction mediated by DNA polymerases is known to require two Mg(II) ions, one

204 polymerase beta, a member of the X family of DNA polymerases, is known to be involved in base excisio

205 DNA polymerase I large (Klenow) fragment showed no detec

206 NA polymerases were investigated: mesophilic DNA polymerase I large (Klenow) fragment, 3'-->5' exo(-)

207 se (TteUvrD) and Bacillus stearothermophilus DNA polymerase I Large Fragment (Bstpol) using a coiled-

208 Nt.CviPII was used in combination with Bst DNA polymerase I large fragment to rapidly amplify anony

209 ures and enzyme kinetic analyses of Bacillus DNA polymerase I large fragment variants complexed with

210 able bacterial (Bacillus stearothermophilus) DNA polymerase I large fragments with DNA primer templat

211 novel polymerase from the well characterized DNA polymerase I-like Thermus thermophilus DNA polymeras

212 DNA polymerase is maintained at stalled replication fork

213 The interaction between UL2 and the DNA polymerase is mediated through the UL30 subunit.

214 DNA damage was also examined using DNA polymerase I-mediated biotin-dATP nick translation (

215 DNA polymerase I-mediated biotin-dATP nick translation (

216 is paradigm, a naturally occurring bacterial DNA polymerase I member isolated from Geobacillus stearo

217 Furthermore, we have found that the viral DNA polymerase is mislocalized to the cytoplasm in both

218 The polymerase and 5'-nuclease components of DNA polymerase I must collaborate in vivo so as to gener

219 We established a DNA polymerase I mutant library, with random substitutio

220 f mutation rates, we generated a panel of 66 DNA polymerase I mutants in Escherichia coli with compar

221 al proof that the D(752) allele in the viral DNA polymerase is necessary and sufficient for expressio

222 ther the Klenow fragment of Escherichia coli DNA polymerase I nor the T7 DNA polymerase, both of whic

223 Neither polA (DNA polymerase I) nor polB (DNA polymerase II) genes are

224 Although the REV3 translesion DNA polymerase is not required for recombination, it int

225 hich rat DNA polymerase beta substitutes for DNA polymerase I of Escherichia coli, we previously isol

226 We previously showed that the DNA polymerase I of Thermus aquaticus (TaqNP) endonucleo

227 For an in vitro assembly reaction, a DNA polymerase is often used either alone for its 3'-5'

228 Adenovirus DNA polymerase is one of three viral proteins and two ce

229 d by the Klenow fragment of Escherichia coli DNA polymerase I or by calf thymus DNA polymerase alpha.

230 Cells deleted for the polA (DNA polymerase I) or priA (primosome) genes are as sensi

231 Escherichia coli DNA polymerase I participates in DNA replication, DNA re

232 We demonstrated that the Klenow fragment (DNA polymerase I) performs translesion synthesis on this

233 The LEXE motif, conserved in eukaryotic type DNA polymerases, is placed close to the polymerization a

234 politana (Tne) DNA polymerase belongs to the DNA polymerase I (Pol I) family.

235 s ability to substitute for Escherichia coli DNA polymerase I (pol I) in the SC18-12 strain, which la

236 The 5' nuclease of DNA polymerase I (Pol I) of Escherichia coli is a member

237 DNA polymerase I (pol I) processes RNA primers during la

238 To increase error rates of DNA polymerase I (Pol I) replication, we introduced poin

239 elity of DNA replication by Escherichia coli DNA polymerase I (pol I) was assessed in vivo using a re

240 irectly visualize single fluorescent labeled DNA polymerase I (Pol) and ligase (Lig) molecules search

241 he 'non-replicative' DNA polymerases, namely DNA polymerase I (polA), II (polB), IV (dinB) and V (umu

242 expressed from an operon encoding a putative DNA polymerase I (PolA1), among other GAS products.

243 ditionally, deletion of the Klenow domain of DNA polymerase I (PolI) resulted in a approximately 35-f

244 t enzyme action depends on replication mode: DNA Polymerase I (PolI)-dependent ColE1 and p15A origins

245 In Bacillus subtilis, DNA polymerase is predominantly located at or near midce

246 Fidelity of DNA polymerases is predominantly governed by an induced

247 DNA polymerase mu (Polmu), an X-family DNA polymerase, is preferentially expressed in secondary

248 m that we previously deduced for Dpo4 and T7 DNA polymerases is preferred for Pol kappa as well, sugg

249 chanism for the observed expansions with Taq DNA polymerase is proposed that does not invoke strand s

250 The 5'-exonuclease domains of the DNA polymerase I proteins of Eubacteria and the FEN1 pro

251 ed to the 5' exonuclease domain of bacterial DNA polymerase I proteins.

252 Sequenase (exo-minus T7 DNA polymerase) is qualitatively similar to exo-minus Kl

253 support a model in which the leading strand DNA polymerase is recruited prior to origin DNA unwindin

254 the difference between low and high fidelity DNA polymerases is related to the efficiency of correct,

255 However, the RB69 DNA polymerase is relatively resistant to the broad-spec

256 n skin cancer formation, we determined which DNA polymerase is responsible for generating UV mutation

257 The Polzeta translesion synthesis (TLS) DNA polymerase is responsible for over 50% of spontaneou

258 polymerase I antibody and addition of excess DNA polymerase I reversed this inhibition.

259 The Klenow fragment of E. coli DNA polymerase I selects its natural substrates, deoxynu

260 Compared to the Klenow fragment of DNA polymerase I, Sequenase could read through homopolym

261 This BER process was dependent upon DNA polymerase I since very-long patch BER was inhibited

262 Primer utilization by T7 DNA polymerase is slower than primer formation.

263 that the Klenow fragment of Escherichia coli DNA polymerase I stopped synthesis mostly after incorpor

264 termining the biochemical properties of this DNA polymerase is structure-function studies of site-spe

265 sive (homologous or heterologous) "trailing" DNA polymerase is sufficient to improve gp41 processivit

266 oding the catalytic subunit of mitochondrial DNA polymerase is sufficient to support physiological va

267 With the Klenow fragment of Escherichia coli DNA polymerase I, synthesis stops at the base immediatel

268 200,000 members) of mutant Thermus aquaticus DNA polymerase I (Taq pol I) was created containing rand

269 200,000 members) of mutant Thermus aquaticus DNA polymerase I (Taq pol I) was created containing rand

270 DNA polymerases including Thermus aquaticus DNA polymerase I (Taq Pol), a thermostable enzyme common

271 s in the Klenow fragment of Escherichia coli DNA polymerase I that interact with the ssDNA overhang o

272 The Taq DNA polymerase is the most commonly used enzyme in DNA s

273 sequence analyses suggest that the class II DNA polymerase is the principal DNA replicative enzyme o

274 e nucleotidyl transfer reaction catalyzed by DNA polymerases is the critical step governing the accur

275 beta (polbeta), a member of the X family of DNA polymerases, is the major polymerase in the base exc

276 not a member of the Y family of error-prone DNA polymerases, is the primary mediator of survival thr

277 ith four polymerases, the Klenow fragment of DNA polymerase I, the Klenow fragment with the proof-rea

278 In whole DNA polymerase I, the polymerase and 5'-nuclease activit

279 The Klenow fragment of Escherichia coli DNA polymerase I then elongates the template-primer by t

280 erase X (pol X), a member of the X family of DNA polymerases, is thought to be involved in base excis

281 distribution was influenced by the ratio of DNA polymerase I to DNA ligase activity in the reaction.

282 DB-dATP showed specific photocrosslinking of DNA polymerase I to DNA.

283 We also investigated the effect on DNA polymerase I to establish whether we could in the fu

284 -slide application of the Klenow fragment of DNA polymerase I to extend unmodified miRNAs hybridized

285 ditionally, Cu(II) chelated PyED outcompetes DNA polymerase I to successfully inhibit template strand

286 he ability of the Klenow fragment of E. coli DNA polymerase I to synthesize and extend the different

287 ne POLG, coding for the catalytic subunit of DNA polymerase, is typically proximal with early ophthal

288 tor of translesion DNA synthesis because the DNA polymerase is unable to extend beyond the incorporat

289 We demonstrate that the DNA polymerase is unable to extend beyond the incorporat

290 appa (Polkappa), a member of the Y-family of DNA polymerases, is unable to insert nucleotides opposit

291 It is also demonstrated that when Taq DNA polymerase is used in the presence of betaine or a p

292 ' exonuclease free (exo-) Klenow fragment of DNA polymerase I was used, dG-N2-3MeE promoted mostly on

293 with the Klenow fragment of Escherichia coli DNA polymerase I, we find that DDI markedly enhances the

294 ce of the J-helix region of Escherichia coli DNA polymerase I, we performed site-directed mutagenesis

295 Various polymerization conditions using DNA polymerase I were examined to determine optimal labe

296 A by the Klenow fragment of Escherichia coli DNA polymerase I when individually substituted for its n

297 d by the Klenow fragment of Escherichia coli DNA polymerase I with better efficiency and fidelity tha

298 Although X-ray crystal structures of DNA polymerase I with substrate dNTPs have revealed key

299 fidelity Klenow fragment of Escherichia coli DNA polymerase I with the proofreading exonuclease activ

300 ween the Klenow fragment of Escherichia coli DNA polymerase I with the proofreading exonuclease inact