ライフサイエンスコーパス: DNA polymerase III

1 th more than just the chromosomal replicase, DNA polymerase III.

2 logy to the E. coli alpha subunit of E. coli DNA polymerase III.

3 the alpha and theta subunits in the E. coli DNA polymerase III.

4 lease proofreading subunit (DnaQ) of E. coli DNA polymerase III.

5 the clamp-loading subunit delta' of E. coli DNA polymerase III.

6 gene, which encodes the enzymatic subunit of DNA polymerase III.

7 olog of the Escherichia coli beta subunit of DNA polymerase III.

8 de insertion by the catalytic subunit of the DNA polymerase III.

9 ession of DnaA protein and the beta clamp of DNA polymerase III.

10 onjunction with chi, an accessory subunit of DNA polymerase III.

11 lesions that typically block the replicative DNA polymerase III.

12 er spontaneous DSBs in a mutant with altered DNA polymerase III.

13 mediated by an unidentified modification of DNA polymerase III.

14 codes for both the tau and gamma subunits of DNA polymerase III.

15 ies a defect in proofreading activity of the DNA polymerase III.

16 naE homologue, encoding the alpha subunit of DNA polymerase III.

17 DNA polymerase III, a decameric 420-kDa assembly, simult

18 In addition, we have found that the DNA polymerase III alpha catalytic subunit interacts str

19 t, an antibiotic that inhibits the PolC-type DNA polymerase III alpha subunit C, versus vancomycin fo

20 The crystal structure of Thermus aquaticus DNA polymerase III alpha subunit reveals that the struct

21 mined the influence of the 3' exonuclease of DNA polymerase III and exonuclease I on deletion via the

22 heir ability to inhibit purified B. subtilis DNA polymerase III and the growth of B. subtilis in cult

23 mp loader delta' subunit of Escherichia coli DNA polymerase III and the hexamerization component of N

24 The alpha subunits of bacterial DNA polymerase III and two distinct family X DNA polymer

25 DnaA boxes around the dnaN (beta subunit of DNA polymerase III) and dnaA genes usually defines the c

26 ved by the gamma-complex in Escherichia coli DNA polymerase III, and indeed our accessory subunit mod

27 a predicted function: dnaE2, a component of DNA polymerase III, and linB, which is similar to 1,3,4,

28 The tau and gamma subunits of DNA polymerase III are both encoded by a single gene in

29 e tau and gamma subunits of Escherichia coli DNA polymerase III, are suppressed by Cs,Sx mutations of

30 NA, UmuD interacts with the alpha subunit of DNA polymerase III at two distinct binding sites, one of

31 The prokaryotic DNA polymerase III beta homodimeric clamp links the repl

32 rve that DnaE2 and ImuB co-localize with the DNA polymerase III beta subunit (beta clamp) in distinct

33 terial sliding clamp (SC), also known as the DNA polymerase III beta subunit, is an emerging antibact

34 dimeric ring-shaped sliding clamp of E. coli DNA polymerase III (beta subunit, homolog of eukaryotic

35 f low-GC gram-positive organisms, be renamed DNA polymerase III C (Pol III C) to denote its origin fr

36 oli DNA polymerase II (B family) and E. coli DNA polymerase III (C family).

37 We conclude from this study that DNA polymerase III catalyzes translesion synthesis acros

38 The prokaryotic DNA polymerase III clamp loader complex loads the beta c

39 ect interaction between the alpha subunit of DNA polymerase III core and the beta sliding clamp.

40 plex or in the presence of gamma complex and DNA polymerase III core components.

41 preventing gamma complex from competing with DNA polymerase III core for binding a newly loaded beta.

42 primer, supports processive DNA synthesis by DNA polymerase III core in the presence of the beta slid

43 On model oligonucleotide templates, DNA polymerase III core is inhibited by SSB.

44 Nevertheless, identical assemblies of the DNA polymerase III core tethered to the beta sliding cla

45 cient overproduction and in vivo assembly of DNA polymerase III core, artificial operons containing t

46 In contrast, DNA polymerase III core, in the absence of deltadelta' a

47 theta is a tightly bound component of the DNA polymerase III core, which contains the alpha subuni

48 DNA polymerase III (DNA pol III) efficiently replicates

49 that inhibit the replication-specific enzyme DNA polymerase III (DNA pol III) of Staphlococcus aureus

50 Mutations that impair DNA polymerase III (DnaE, DnaQ subunits) or the replicat

51 structure, it is proposed that it be renamed DNA polymerase III E (Pol III E) to accurately reflect i

52 4 isolates shared an E9G substitution in the DNA polymerase III e-subunit active site, resulting in a

53 Only mutations in functions associated with DNA Polymerase III elevated deletion rates in our assays

54 which encode DNA polymerase I and the second DNA polymerase III enzyme, respectively.

55 of MMLV RT, including p53, Escherichia coli DNA polymerase III epsilon subunit, and the proofreading

56 dnaQ encodes DNA polymerase III epsilon subunit, the proofreading sub

57 the proofreading function carried out by the DNA polymerase III epsilon subunit.

58 ication misalignment events are sensitive to DNA polymerase III exonuclease, whereas SCE-associated e

59 Bacterial DNA polymerase III (family C DNA polymerase), the princi

60 ) and psi (psi) subunits of Escherichia coli DNA polymerase III form a heterodimer that is associated

61 e impaired in 3'-->5'exonuclease activity of DNA polymerase III, frame-shift mutagenesis increased 5-

62 a deletion approach on the alpha subunit of DNA polymerase III from Escherichia coli, we show that t

63 By purifying DNA polymerase III* from TSM-induced and control cells,

64 The Escherichia coli DNA polymerase III gamma complex clamp loader assembles

65 The Escherichia coli DNA polymerase III gamma complex loads the beta clamp on

66 bunit of the clamp-loader complex of E. coli DNA polymerase III has been determined.

67 Mg2+ in the active site of Escherichia coli DNA polymerase III have been identified as Asp401, Asp40

68 icating that tau's unique ability to bind to DNA polymerase III holding chipsi in the same complex is

69 le of accessory subunits of Escherichia coli DNA polymerase III holoenzyme (HE) in determining chromo

70 The in vitro fidelity of Escherichia coli DNA polymerase III holoenzyme (HE) is characterized by a

71 The DNA polymerase III holoenzyme (HE) is the primary replic

72 There is a controversy as to whether or not DNA polymerase III holoenzyme (Pol III HE) contains gamm

73 or subassemblies of the bacterial replicase, DNA polymerase III holoenzyme (Pol III HE).

74 uires the UmuD'2C complex, RecA protein, and DNA polymerase III holoenzyme (pol III).

75 trand DNA replication in the presence of the DNA polymerase III holoenzyme all had the same protein c

76 subunit of the Escherichia coli replicative DNA polymerase III holoenzyme and encodes an exonuclease

77 freading 3' exonuclease (epsilon subunit) of DNA polymerase III holoenzyme and is a critical determin

78 Interaction between the tau subunit of the DNA polymerase III holoenzyme and the DnaB helicase is c

79 t between the dimeric tau subunit within the DNA polymerase III holoenzyme and the DnaB helicase is r

80 tant for DNA replication, the chi subunit of DNA polymerase III holoenzyme and the PriA helicase.

81 g clamp protein beta of the Escherichia coli DNA polymerase III holoenzyme are shown to function as t

82 The single endogenous DnaX complex within DNA polymerase III holoenzyme assembles beta onto both t

83 We demonstrate here that the E. coli DNA polymerase III holoenzyme assembles into a particle

84 The gamma complex of the Escherichia coli DNA polymerase III holoenzyme assembles the beta sliding

85 toward the beta subunit of Escherichia coli DNA polymerase III holoenzyme by mutation of a phenylala

86 Escherichia coli DNA polymerase III holoenzyme contains 10 different subu

87 The catalytic core of Escherichia coli DNA polymerase III holoenzyme contains three subunits: a

88 pose a model in which strand displacement by DNA polymerase III holoenzyme depends upon a Pol III-tau

89 The minimal Tth DNA polymerase III holoenzyme displays an elongation rat

90 The tau and gamma proteins of the DNA polymerase III holoenzyme DnaX complex are products

91 The DNA Polymerase III holoenzyme forms initiation complexes

92 ed the in vitro fidelity of Escherichia coli DNA polymerase III holoenzyme from a wild-type and a pro

93 ng the poorly conserved delta subunit of the DNA polymerase III holoenzyme from all sequenced bacteri

94 Subunit interactions known to occur in DNA polymerase III holoenzyme from mesophilic bacteria i

95 SB's association with the chi subunit of the DNA polymerase III holoenzyme has been proposed to confe

96 components and functions of Escherichia coli DNA polymerase III holoenzyme have been studied extensiv

97 We report here that the DNA polymerase III holoenzyme in a stalled E. coli repli

98 Purified gp8 inhibits DNA polymerization by DNA polymerase III holoenzyme in vitro by interfering wi

99 Probing with surface plasmon resonance, DNA polymerase III holoenzyme initiation complexes were

100 ngs are consistent with chipsi strengthening DNA polymerase III holoenzyme interactions with the SSB-

101 Escherichia coli DNA polymerase III holoenzyme is a multisubunit composit

102 processive DNA synthesis by Escherichia coli DNA polymerase III holoenzyme is achieved by the direct

103 The DNA polymerase III holoenzyme is composed of 10 subunits

104 The DnaX complex subassembly of the DNA polymerase III holoenzyme is comprised of the DnaX p

105 rse of this work, we discovered that E. coli DNA polymerase III holoenzyme is itself capable of carry

106 ion of the theta subunit of Escherichia coli DNA polymerase III holoenzyme is not well established.

107 DNA polymerase III holoenzyme is responsible for chromos

108 lity DNA synthesis that is characteristic of DNA polymerase III holoenzyme is the 3'-->5' proofreadin

109 DNA polymerase III holoenzyme is the major replicative e

110 Escherichia coli DNA polymerase III holoenzyme is the replicative enzyme

111 e in their interaction with the helicase and DNA polymerase III holoenzyme lacking the tau subunit so

112 imers are formed, two molecules of the large DNA polymerase III holoenzyme machinery assemble into th

113 e polymerase core (alphaepsilontheta) of the DNA polymerase III holoenzyme map to widely separated lo

114 Studies of the DNA polymerase III holoenzyme of Escherichia coli suppor

115 essive replication reaction catalyzed by the DNA polymerase III holoenzyme on single-stranded DNA tem

116 med in the absence of the tau subunit of the DNA polymerase III holoenzyme produce shorter leading an

117 esults imply that T. thermophilis utilizes a DNA polymerase III holoenzyme replication machinery simi

118 tadelta'chi psi) within the Escherichia coli DNA polymerase III holoenzyme serves to load the dimeric

119 beads to investigate the effects of various DNA polymerase III holoenzyme subunits on the kinetics o

120 ainst the alpha catalytic subunit of E. coli DNA polymerase III holoenzyme to distinguish a novel pol

121 mp encircles the primer-template and tethers DNA polymerase III holoenzyme to DNA for processive repl

122 for DnaB binding, which leads to assembly of DNA polymerase III holoenzyme to form the replisome.

123 interaction with the alpha subunit, allowing DNA polymerase III holoenzyme to synthesize both leading

124 beta sliding clamp encircles DNA and tethers DNA polymerase III holoenzyme to the template for high p

125 These ring-shaped clamps tether DNA polymerase III holoenzyme to the template, facilitat

126 u subunit of the replicative polymerase (the DNA polymerase III holoenzyme) and the replication fork

127 aA operon, including DnaA, the beta clamp of DNA polymerase III holoenzyme, and RecF.

128 dnaN, and recF encoding DnaA, beta clamp of DNA polymerase III holoenzyme, and RecF.

129 , encoding the tau and gamma subunits of the DNA polymerase III holoenzyme, as a high-copy suppressor

130 hi form a discrete complex separate from the DNA polymerase III holoenzyme, but little is known about

131 In Escherichia coli DNA polymerase III holoenzyme, chi and Psi are tightly a

132 The E. coli replicase, DNA polymerase III holoenzyme, contains two polymerases

133 m of the E. coli replicative DNA polymerase, DNA polymerase III holoenzyme, function in translesion s

134 the editing function of the Escherichia coli DNA polymerase III holoenzyme, i.e., the DnaQ/MutD prote

135 codes both the gamma and tau subunits of the DNA polymerase III holoenzyme, identified the dnaX trans

136 Studies of the E. coli replicase, DNA polymerase III holoenzyme, in chain elongation with

137 as measured by processive replication by the DNA polymerase III holoenzyme, indicates a minimal requi

138 oteins promote the engagement of primase and DNA polymerase III holoenzyme, initiating semi-discontin

139 ocessive DNA replication by Escherichia coli DNA polymerase III holoenzyme, is assembled onto DNA by

140 The Escherichia coli chromosomal replicase, DNA polymerase III holoenzyme, is highly processive duri

141 codes both the gamma and tau subunits of the DNA polymerase III holoenzyme, is subject to cell cycle

142 Here we show that delta, a single subunit of DNA polymerase III holoenzyme, opens beta and slips it o

143 ta' subunits are essential components of the DNA polymerase III holoenzyme, required for assembly and

144 , encoding the tau and gamma subunits of the DNA polymerase III holoenzyme, that causes extreme cell

145 a specialized multicomponent replicase, the DNA polymerase III holoenzyme, that consist of three ess

146 The epsilon subunit of Escherichia coli DNA polymerase III holoenzyme, the enzyme primarily resp

147 nd gamma, are found co-assembled in purified DNA polymerase III holoenzyme, the pathway of assembly i

148 as a sliding clamp to tether the replicase, DNA polymerase III holoenzyme, to DNA.

149 showed that the replicative polymerase, the DNA polymerase III holoenzyme, was indeed a dimer with t

150 sivity factor beta(2) onto primed DNA in the DNA polymerase III holoenzyme-catalyzed reaction.

151 esistance to high levels of glutamate on the DNA polymerase III holoenzyme-catalyzed reaction.

152 on and abolished the replication activity of DNA polymerase III holoenzyme.

153 mp loader, RFC, and the structure of E. coli DNA polymerase III holoenzyme.

154 t is part of the Escherichia coli replicase, DNA polymerase III holoenzyme.

155 osely links primase action with extension by DNA polymerase III holoenzyme.

156 eplication of the Escherichia coli genome by DNA polymerase III holoenzyme.

157 the catalytic alpha subunit of the bacterial DNA Polymerase III holoenzyme.

158 er for the synthesis of DNA chain by E. coli DNA polymerase III holoenzyme.

159 s to form the E. coli chromosomal replicase, DNA polymerase III holoenzyme.

160 composed solely of E. coli DnaB helicase and DNA polymerase III holoenzyme.

161 dnaX gene, the gamma and tau subunits of the DNA polymerase III holoenzyme.

162 d, as was their ability to interact with the DNA polymerase III holoenzyme.

163 f the beta clamp-loading DnaX complex in the DNA polymerase III holoenzyme.

164 ctions as a sliding clamp for the replicase, DNA polymerase III holoenzyme.

165 freading epsilon subunit of Escherichia coli DNA polymerase III holoenzyme.

166 ene, encoding the alpha-catalytic subunit of DNA polymerase III holoenzyme.

167 k formation by the restart primosome and the DNA polymerase III holoenzyme.

168 nits of the DnaX complex of Escherichia coli DNA polymerase III holoenzyme: tau, gamma, delta, delta'

169 The delta' subunit of the DNA polymerase-III holoenzyme is a key component of the

170 on and abolished the replication activity of DNA polymerase-III holoenzyme.

171 We show that the alpha polymerase subunit of DNA polymerase III interacts with the beta-clamp via its

172 Replication by Escherichia coli DNA polymerase III is disrupted on encountering DNA dama

173 To determine whether DNA polymerase III is involved in the TSM phenotype, we

174 These findings suggest that DNA polymerase III is modified in TSM-induced cells.

175 e 28 kDa epsilon subunit of Escherichia coli DNA polymerase III is the exonucleotidic proofreader res

176 DNA Polymerase III is the main replicative polymerase of

177 s techniques to identify the region(s) where DNA polymerase III-mediated replication has historically

178 )C with the epsilon and the beta subunits of DNA polymerase III might help to regulate DNA replicatio

179 dnaX encoding the gamma and tau subunits of DNA polymerase III mimic dnaK phenotypes and are genetic

180 dGTP analogues which selectively inhibit the DNA polymerase III of Bacillus subtilis and other Gram-p

181 re required for binding of the beta-clamp to DNA polymerase III of Escherichia coli, a polymerase of

182 ur SIPs (RecO, PriC, PriA and chi subunit of DNA polymerase III) of three peptides containing the aci

183 freading epsilon-subunit of Escherichia coli DNA polymerase III on the genetic instabilities of the C

184 he hydrophobic cleft on the clamp that binds DNA polymerase III or a clamp-binding motif in SocB.

185 notype is mediated through a modification of DNA polymerase III or the activation of a previously uni

186 lication activity and that a modification of DNA polymerase III or the expression of a previously uni

187 e-stranded binding protein (SSB), and either DNA polymerases III or II.

188 presence of an antimutator allele of E. coli DNA polymerase III (Pol III) (dnaE915), but not in the p

189 rains carrying both an antimutator allele of DNA polymerase III (Pol III) alpha-subunit (dnaE915) and

190 The dnaX gene encodes the tau subunit of DNA polymerase III (Pol III) holoenzyme, the enzyme resp

191 ction of the dnaX-encoded tau subunit of the DNA polymerase III (Pol III) holoenzyme.

192 The fidelity of Escherichia coli DNA polymerase III (pol III) is measured and the effects

193 DNA polymerase III (Pol III) is the catalytic alpha subu

194 DNA polymerase III (pol III) of Gram-positive eubacteria

195 nce loaded, beta(148-152) was proficient for DNA polymerase III (Pol III) replication in vitro.

196 ene, encoding the catalytic alpha-subunit of DNA polymerase III (pol III) was isolated by genetic com

197 nts (1-2 kb) and utilize a highly processive DNA polymerase III (pol III), which is held to DNA by a

198 ucleolytic proofreading (epsilon) subunit of DNA polymerase III (Pol III).

199 ucture of the catalytic alpha-subunit of the DNA polymerase III (Pol IIIalpha) holoenzyme bound to pr

200 the alpha (catalytic) subunit of replicative DNA polymerase III (PolIII).

201 The epsilon subunit of Escherichia coli DNA polymerase III possesses 3'-exonucleolytic proofread

202 s of Nuc and epsilon186, an Escherichia coli DNA polymerase III proofreading domain, providing a comm

203 ivated form of UmuD (UmuD'), UmuC, RecA, and DNA polymerase III proteins.

204 erial DnaA (replication initiator) and DnaE (DNA polymerase III) proteins respectively.

205 sistent with the in vivo requirement for two DNA polymerase III replicases for B. subtilis chromosoma

206 oteomic/genomic approach to characterize the DNA polymerase III replication apparatus of the extreme

207 DnaB is the helicase associated with the DNA polymerase III replication fork in Escherichia coli.

208 The Escherichia coli helicase, YoaA, and DNA polymerase III subunit, chi, form a complex (YoaA-ch

209 Escherichia coli DNA polymerase III subunits tau and gamma are produced f

210 nsfer determinants and genes encoding likely DNA polymerase III subunits, but it lacks an obvious can

211 s as its mechanistic basis the alteration of DNA polymerase III such that it becomes capable of repli

212 ssion of the epsilon proofreading subunit of DNA polymerase III suppresses umuDC-mediated cold sensit

213 The Escherichia coli DNA polymerase III tau and gamma subunits are single-str

214 mbly of tau and gamma, suggesting a role for DNA polymerase III' [tau(2)(pol III core)(2)] in the ass

215 , the CDC2 gene encodes the large subunit of DNA polymerase III, the analogue of mammalian DNA polyme

216 rocessivity of the isolated alpha subunit of DNA polymerase III, the product of the dnaE gene, from t

217 e P1 can substitute for the Escherichia coli DNA polymerase III theta subunit, as evidenced by its st

218 tau binds DNA polymerase III tightly; gamma does not.

219 tching places on the beta sliding clamp with DNA polymerase III to bypass the template damage, or act

220 ent per cell, thus allowing partially active DNA polymerase III to complete replication of enough chr

221 The beta clamp tethers DNA polymerase III to DNA and enhances the efficiency of

222 , UmuD' acts in concert with UmuC, RecA, and DNA polymerase III to facilitate the process of transles

223 portion of the catalytic subunit (alpha) of DNA polymerase III to the C-terminal half, downstream of

224 proteins in this mutagenic process are RecA, DNA polymerase III, UmuD, UmuD' and UmuC (umu: UV mutage

225 The tau subunit dimerizes DNA polymerase III via interaction with the alpha subuni

226 ts, UmuD and UmuD' interact differently with DNA polymerase III: whereas uncleaved UmuD interacts mor

227 umption of DNA synthesis occurs by replacing DNA polymerase III, which is bound to DNA by the beta-sl

228 rase II and inhibits proofreading by E. coli DNA polymerase III, while permitting templated DNA synth

229 hromosome replication is mainly catalyzed by DNA polymerase III, whose beta subunits enable rapid pro

230 c C-tau binds the alpha catalytic subunit of DNA polymerase III with a 1:1 stoichiometry.

231 vitro studies of the interactions of E. coli DNA polymerase III with these adducts have also been und

232 lication being catalyzed by the a subunit of DNA polymerase III within the bacterial replisome.