ライフサイエンスコーパス: DNA polymerase epsilon

1 1, Mms19 and Cdc20 (the catalytic subunit of DNA polymerase-epsilon).

2 markedly enhances the synthetic activity of DNA polymerase epsilon.

3 interacts with the DNA replication protein, DNA polymerase epsilon.

4 of DNA replication via its interaction with DNA polymerase epsilon.

5 editing 3' exonuclease domain of eukaryotic DNA polymerase epsilon.

6 ication proteins including Mcm10, RFC140 and DNA polymerase epsilon 255 kDa subunit in S-phase.

7 Yeast strains with this DNA polymerase epsilon allele have elevated rates of T t

8 cerevisiae POL2 and POL3 genes, which encode DNA polymerase epsilon and delta, respectively, and show

9 er found recently to bind the leading strand DNA polymerase epsilon and load PCNA onto leading strand

10 ana mutant of the POL2A catalytic subunit of DNA polymerase epsilon and show that POL2A is required t

11 h and stimulate the polymerase activities of DNA polymerase epsilon and the DNA polymerase alpha-prim

12 DNA substrate, the combined action of human DNA polymerase epsilon and the human CMG complex leads t

13 lly with mutations affecting the function of DNA polymerase epsilon and the S-phase checkpoint protei

14 lpha) and Ctf4 recruitment without affecting DNA polymerases epsilon and delta or the CMG helicase.

15 Most current evidence indicates that DNA polymerases epsilon and delta, respectively, perform

16 This produces a replisome that lacks DNA polymerase epsilon, and although cells are viable, t

17 Since DNA polymerase epsilon appears to be involved in DNA rep

18 of three molecular origin firing processes, DNA polymerase epsilon arrival, TopBP1 ejection and GINS

19 repair of mismatches made by leading-strand DNA polymerase epsilon as compared to lagging-strand DNA

20 lex (or Tim alone) is able to associate with DNA polymerase epsilon bound to a 40-/80-mer DNA ligand.

21 to budding yeast Dpb11, which interacts with DNA polymerase epsilon) but not on Rhp9 (similar to budd

22 2 or the removal of the C-terminal domain of DNA polymerase epsilon by trypsin digestion or competiti

23 lude that the 3' --> 5' exonuclease of human DNA polymerase epsilon can remove 3'-terminal F-ara-AMP

24 The human DNA polymerase epsilon catalytic subunit consists of a 1

25 Additionally, maximal rates only occur when DNA polymerase epsilon catalyzes leading-strand synthesi

26 tation experiments revealed that recombinant DNA polymerase epsilon directly interacts with either Ti

27 o the C-terminus of the catalytic subunit of DNA polymerase epsilon (DNA pol epsilon), to a region th

28 stablishing leading-strand synthesis, before DNA polymerase epsilon engagement.

29 In eukaryotes DNA polymerase epsilon (epsilon) synthesises the leading

30 nteracts with Pol2, the catalytic subunit of DNA polymerase epsilon, essential for leading-strand DNA

31 DNA by the 3' --> 5'-exonuclease activity of DNA polymerase epsilon from human leukemia CEM cells.

32 In contrast, DNA polymerase epsilon, functioned with the chromatin re

33 eplicative helicase, and Dpb3/4, subunits of DNA polymerase epsilon, govern parental histone H3-H4 de

34 After DNA polymerase epsilon had been incubated with 3'-F-ara-

35 inetic evaluation demonstrated that although DNA polymerase epsilon has a higher affinity for F-ara-A

36 Ps in DNA could be problematic because yeast DNA polymerase epsilon has difficulty bypassing a single

37 After extension of the upstream primer with DNA polymerase epsilon, human DNA ligase I was able to c

38 eracted with Pol2p, the catalytic subunit of DNA polymerase epsilon, in vivo.

39 We show that DNA polymerase epsilon is not able to replace DNA polyme

40 Consistent with the hypothesis that DNA polymerase epsilon is the leading-strand enzyme, we

41 uggested that either DNA polymerase delta or DNA polymerase epsilon may be involved.

42 r synthesis, we suggest that MDM2 binding to DNA polymerase epsilon might be part of a reconfiguratio

43 ocus, which encodes the catalytic subunit of DNA polymerase epsilon of Arabidopsis thaliana, causes a

44 atin, and it associates with Mcm2p, RPA, and DNA polymerase epsilon only during S phase.

45 rmore, the 3' --> 5' exonuclease activity of DNA polymerase epsilon or wild-type p53 protein was inef

46 ication and reveal a pattern indicating that DNA polymerase epsilon participates in leading-strand DN

47 ontinuous synthesis of the leading strand by DNA polymerase epsilon (Pol epsilon) and discontinuous s

48 ombe cdc20+ encodes the catalytic subunit of DNA polymerase epsilon (pol epsilon) and that this enzym

49 DNA polymerase delta (Pol delta) and DNA polymerase epsilon (Pol epsilon) are both required f

50 erance of evidence supports a model in which DNA polymerase epsilon (Pol epsilon) carries out the bul

51 type and exonuclease-deficient four-subunit DNA polymerase epsilon (Pol epsilon) complex from Saccha

52 Genetic evidence suggests that DNA polymerase epsilon (Pol epsilon) has a noncatalytic

53 DNA polymerase epsilon (pol epsilon) has been implicated

54 DNA polymerase epsilon (Pol epsilon) is a multi-subunit

55 DNA polymerase epsilon (pol epsilon) is a multiple subun

56 DNA polymerase epsilon (Pol epsilon) is a replicative DN

57 DNA polymerase epsilon (Pol epsilon) is believed to play

58 accharomyces pombe, the catalytic subunit of DNA polymerase epsilon (Pol epsilon) is encoded by cdc20

59 Saccharomyces cerevisiae DNA polymerase epsilon (pol epsilon) is essential for ch

60 DNA polymerase epsilon (Pol epsilon) is required for gen

61 es during DNA replication has suggested that DNA polymerase epsilon (Pol epsilon) may also play a rol

62 most frequently recurring cancer-associated DNA polymerase epsilon (Pol epsilon) mutation is a P286R

63 Human DNA polymerase epsilon (pol epsilon) normally contains a

64 DNA polymerase epsilon (pol epsilon) was purified to app

65 The replicative DNA polymerase epsilon (Pol epsilon) was shown to activa

66 oliferating cell nuclear antigen (PCNA), and DNA polymerase epsilon (Pol epsilon), are reduced at sta

67 HeLa DNA polymerase epsilon (pol epsilon), possibly involved

68 HeLa DNA polymerase epsilon (pol epsilon), possibly involved

69 ter understand the functions and fidelity of DNA polymerase epsilon (Pol epsilon), we report here on

70 Genetic interactions were examined among DNA polymerase epsilon (pol2-4) and delta (pol3-01) muta

71 he large subunit of Saccharomyces cerevisiae DNA polymerase epsilon, Pol2, comprises two essential fu

72 lfur cluster (ISC) biosynthesis and identify DNA polymerase epsilon (POLE) as an ISC-containing prote

73 clease domain mutations in the gene encoding DNA polymerase epsilon (POLE) have incredibly high mutat

74 Molecular subtypes of EC were assigned using DNA polymerase epsilon (POLE) hotspot mutations and immu

75 DNA polymerase epsilon (PolE) in an enzyme essential for

76 Here we show that the catalytic subunit of DNA polymerase epsilon (POLE) is essential for DNA integ

77 ted) cancers caused by mutations that impair DNA polymerase epsilon (POLE) proofreading.

78 rom this individual identified a mutation in DNA polymerase epsilon (POLE) that associated with an ul

79 ns in the proofreading exonuclease domain of DNA polymerase epsilon (POLE-exo*) exhibit a novel mutat

80 veal that the non-catalytic subunit of human DNA polymerase epsilon (POLE2) also serves as a general

81 e that the loss of the accessory subunits of DNA polymerase epsilon, POLE3 and POLE4, sensitizes cell

82 observed in EC with a pathogenic mutation of DNA polymerase-epsilon (POLEmut EC).

83 Unexpectedly, the leading strand DNA polymerase epsilon (Polepsilon) arrived at telomeres

84 Alterations in the exonuclease domain of DNA polymerase epsilon (Polepsilon) cause ultramutated t

85 Dpb4p is also a subunit of the DNA polymerase epsilon (polepsilon) complex, and Dls1p i

86 The eukaryotic leading-strand DNA polymerase epsilon (Polepsilon) is a dual-function e

87 DNA polymerase epsilon (Polepsilon) is a key enzyme for

88 DNA polymerase epsilon (polepsilon) is a key player in D

89 DNA polymerase epsilon (Polepsilon) is a large, four-sub

90 DNA polymerase epsilon (Polepsilon) is a multi-subunit p

91 In eukaryotic DNA replication, DNA polymerase epsilon (Polepsilon) is responsible for l

92 DNA polymerase epsilon (Polepsilon) performs bulk synthe

93 ly incompatible with simultaneous binding of DNA polymerase epsilon (Polepsilon) to GINS when bound t

94 DNA polymerase epsilon (Polepsilon), one of the three ma

95 quires efficient leading strand synthesis by DNA Polymerase Epsilon (Polepsilon).

96 d substitutions in the exonuclease domain of DNA polymerase epsilon (Polepsilon).

97 During eukaryotic replication, DNA polymerases epsilon (Polepsilon) and delta (Poldelta

98 e mutations of the POL2 and MSH2 genes, both DNA polymerase epsilon proofreading and mismatch repair

99 atures for defective DNA mismatch repair and DNA polymerase epsilon proofreading deficiency, along wi

100 5) but much greater than the contribution of DNA polymerase epsilon proofreading in longer runs.

101 avoidance was found to be similar to that of DNA polymerase epsilon proofreading in short homonucleot

102 esidues 265-605) that associates with Cdc45, DNA polymerase epsilon, replication protein A, and two r

103 cerevisiae proteins Msh2-Msh6 or Msh2-Msh3, DNA polymerase epsilon, RFC, PCNA, RPA, and Mlh1-Pms1 (h

104 During DNA replication, DNA polymerase epsilon subunit DPB3/DPB4 and DNA replica

105 (also known as Raf2/Clr7/Cmc2), or Cdc20, a DNA polymerase epsilon subunit, results in dissociation

106 We constructed a derivative of yeast DNA polymerase epsilon that retains high replication act

107 jacent normal deoxynucleotide was cleaved by DNA polymerase epsilon, the reaction products appeared t

108 art of a reconfiguration process that allows DNA polymerase epsilon to associate with repair/recombin

109 dress this possibility, the ability of human DNA polymerase epsilon to incorporate ribonucleotides wa

110 tch base excision repair in vitro using calf DNA polymerase epsilon to provide strand displacement sy

111 ication by tethering DNA polymerase delta or DNA polymerase epsilon to the DNA template.

112 ers was observed after the addition of fresh DNA polymerase epsilon to the reaction.

113 Escherichia coli stimulated the activity of DNA polymerase epsilon up to 10- and 40-fold, respective

114 that budding yeast Ctf18-RFC associates with DNA polymerase epsilon, via an evolutionarily conserved

115 he excision substrates, we demonstrated that DNA polymerase epsilon was unable to effectively remove

116 rpin structures are most inhibitory to yeast DNA polymerase epsilon, whereas yeast and human Pol zeta

117 lity of CDT1 proteins to form complexes with DNA polymerase epsilon, which functions in DNA replicati

118 ucleoside triphosphate concentrations, yeast DNA polymerase epsilon, which is implicated in leading s

119 with nonorigin DNA with similar kinetics as DNA Polymerase epsilon, which is present at DNA replicat

120 recently that the Mrc1 subunit of RPCs binds DNA polymerase epsilon, which synthesises the leading st

121 how the Cdc45-MCM-GINS helicase is linked to DNA polymerase epsilon, which synthesizes the leading st