ライフサイエンスコーパス: DNMT1

1 m infancy onward by DNA methyltransferase 1 (DNMT1).

2 in cooperation with DNA methyltransferase 1 (DNMT1).

3 maintenance enzyme DNA methyltransferase 1 (DNMT1).

4 actors as the metazoan maintenance methylase Dnmt1.

5 tics and RNA inhibition of recombinant human DNMT1.

6 ing pathways mediating the flow-induction of DNMT1.

7 S-phase by the maintenance methyltransferase Dnmt1.

8 e genome that was rescued by transfection of DNMT1.

9 Np95 is deleted alone or in combination with Dnmt1.

10 ast two of its known targets, namely ID4 and DNMT1.

11 ent degradation of the DNA methyltransferase DNMT1.

12 tate (TS) of the reaction catalyzed by human DNMT1.

13 ransfer step is chemically rate-limiting for DNMT1.

14 HDAC1 and DNA methyl transferases DNMT3b and DNMT1.

15 ol the recruitment and enzymatic activity of DNMT1.

16 lling is maintained by DNA methyltransferase DNMT1.

17 against a possible excessive methylation by DNMT1.

18 ) and maintenance activity ('stabilizer') of DNMT1.

19 egulation was inhibited by overexpression of DNMT1.

20 unced at CpG sites with flanks disfavored by DNMT1.

21 nes and suppression of TICs was dependent on DNMT1.

22 ming and down-regulation of WNT2 mediated by DNMT1.

23 ut not the maintenance DNA methyltransferase Dnmt1.

24 ing is mediated by DNA methyltransferase1/3 (DNMT1/3)-, histone deacetylase 1/2/4 (HDAC1/2/4)-, Setdb

25 DNA, including the DNA methyltransferases 1 (DNMT1), 3A (DNMT3A) and 3B (DNMT3B), are indispensable f

26 Deletion of PRC2, Dnmt1/3a/3b or Mecp2 in ESCs leads to an increase in the

27 EMENT In the present study, we reported that DNMT1, a canonical DNA maintenance methyltransferase, is

28 We show here that DNMT1, a canonical maintenance methyltransferase, acts a

29 domain of maintenance DNA methyltransferase DNMT1, a module known to bind the ubiquitylated H3 (H3Ub

30 However, while IAPs are derepressed in Dnmt1-ablated embryos and embryonic stem cells (ESCs), t

31 ons as well as implicating RNA in regulating DNMT1 activity and correct levels of genomic methylation

32 y, our results demonstrate a requirement for dnmt1 activity in the regulation of RSC proliferation, g

33 led gDMD-like sequences requiring continuous DNMT1 activity to sustain a highly methylated state.

34 ed in aberrant DNA methylation by inhibiting DNMT1 activity, resulting in altered gene expression.

35 rocessing of RNA is necessary to ensure full DNMT1 activity.

36 NA sequences flanking the target CpG site on DNMT1 activity.

37 gical inhibition or genetic knockdown of DRG DNMT1 alleviated nerve injury-induced pain hypersensitiv

38 The expression of G9a and DNMT1, along with that of their molecular adaptor ubiqui

39 s, which in fibroblasts results in increased DNMT1 amount.

40 BM gamma-H2AX (DNA repair/damage marker) and DNMT1 analyses.

41 tformin post FAE affect memory via elevating Dnmt1 and consequently normalizing hippocampal Dio3 and

42 n mice, reminiscent of a genetic knockout of Dnmt1 and could substantially suppress intestinal polyp

43 e maintenance cytosine DNA methyltransferase DNMT1 and de novo methyltransferase DNMT3b cooperate to

44 astrulation stage; however, the functions of Dnmt1 and DNA methylation in organogenesis remain unclea

45 and in vivo, and that DNA methyltransferases Dnmt1 and Dnmt3a are highly enriched in the nephrogenic

46 of TGF-beta1 to increase expression of both DNMT1 and DNMT3a demonstrates a novel means by which TGF

47 Interestingly, we found that depletion of DNMT1 and DNMT3A had opposite effects on circadian perio

48 a cellular reprograming by downregulation of DNMT1 and DNMT3A in vivo.

49 trate that TGF-beta1 increases expression of DNMT1 and DNMT3a through different post-transcriptional

50 The increases in DNMT1 and DNMT3a were dependent on TGF-beta1 activation

51 R1(+) CLL cells leading to downregulation of DNMT1 and DNMT3A, modulation of global DNA methylation,

52 tein expression, but not RNA levels, of both DNMT1 and DNMT3a.

53 We show that DNMT1 and DNMT3a/3b activities work complementarily and

54 erve an unexpected division of labor between DNMT1 and DNMT3a/3b in suppressing retrotransposon long

55 catalytically active DNA methyltransferases (DNMT1 and DNMT3A/B) require accessory proteins such as U

56 through disruption of DNA methyltransferases DNMT1 and DNMT3B and pharmacologic inhibition with 5-Aza

57 is of functional importance to induction of DNMT1 and DNMT3b and, in turn, changes in DNA methylatio

58 UHRF1, DNMT1 and DNMT3B are upregulated in germinal centre B ce

59 These results demonstrate that Dnmt1 and Dnmt3b cooperate to maintain DNA methylation a

60 Ablation of both Dnmt1 and Dnmt3b in the intestinal epithelium is lethal,

61 We show that MUC1-C occupies the DNMT1 and DNMT3b promoters in complexes with NF-kappaB p

62 Elevated recruitment of Dnmt1 and Dnmt3b to the Ogg1 promoter in acrolein treate

63 s in complexes with NF-kappaB p65 and drives DNMT1 and DNMT3b transcription.

64 lls activated STAT3, increased expression of DNMT1 and DNMT3b, and enhanced survival.

65 strate that MUC1-C induces the expression of DNMT1 and DNMT3b, but not DNMT3a, in breast and other ca

66 lude activation of the key factors STAT3 and DNMT1 and expression of CD30 (the hallmark of anaplastic

67 We demonstrated that DNMT1 and KIT form a positive regulatory loop, in which

68 Overexpression of DNMT1 and KIT is prevalent in lung cancer, yet the under

69 in lung cancer pathogenesis, whether and how DNMT1 and KIT orchestrate lung tumorigenesis are unclear

70 d analysis of three truncated targets, BRD4, DNMT1 and NGLY1, revealed partial preservation of protei

71 Conditional deletions reveal that both Dnmt1 and Np95 are essential for maintenance DNA methyla

72 ined 2 small molecules-decitabine to deplete DNMT1 and tetrahydrouridine (THU) to inhibit cytidine de

73 d the up-regulation of DNA methyltransferase DNMT1 and tyrosine-protein kinase KIT, the enhanced phos

74 an up-regulation of DNA methyltransferase 1 (DNMT1) and a global hypermethylation in vascular endothe

75 MDR]) that recruits DNA methyltransferase 1 (Dnmt1) and provokes methylation of the Gata1 gene enhanc

76 , are replicated by DNA methyltransferase 1 (DNMT1) and ubiquitin-like containing PHD and RING finger

77 uhrf1 mutants are phenocopied by mutation of dnmt1, and Dnmt1 knockdown in uhrf1 mutants enhances the

78 ased expression of the senescence regulator, DNMT1, and increased expression of the senescence marker

79 roteins, including histone H3, p53, E2F, and Dnmt1, and is involved in the regulation of gene express

80 s revealed the association of HDAC1, DNMT3b, DNMT1, and mSin3A with SNAIL.

81 icant and correlative overexpression of G9a, DNMT1, and UHRF1 in HCCs in association with poor progno

82 Moreover, tumour EZH2 and DNMT1 are negatively associated with tumour-infiltrating

83 transferase partner DNA methyltransferase I (DNMT1) are critical for the restriction of EBNA and LMP

84 DNMTs), and the two DNMT families, DNMT3 and DNMT1, are responsible for methylation establishment and

85 and in vivo tumorigenic potential, defining DNMT1 as a candidate CSC therapeutic target.

86 We also identify DNMT1 as a factor that induces SAHFs by promoting HMGA2

87 Cas9-mediated screen, which revealed Myc and Dnmt1 as two factors preventing the transition.

88 f proteins involved in this process, such as DNMT1, as a new crucial role of the SCMC for mammalian r

89 cRNA, TCONS_00023265, which we named DACOR1 (DNMT1-associated Colon Cancer Repressed lncRNA 1), shows

90 our results demonstrate that deregulation of DNMT1-associated lncRNAs contributes to aberrant DNA met

91 ss also was associated with up-regulation in DNMT1-associated methylation of the Cnr1 promoter and do

92 de from its role in the CXXC domain-mediated DNMT1 autoinhibition, serves as an important regulatory

93 Lowering DNMT1 below a threshold level is required for maximal lo

94 a dominant role for DNA methyltransferase 1 (DNMT1) but also distinct roles of 3A and 3B in genome-wi

95 that IL-6 induced proteasomal degradation of DNMT1, but not of DNMT3A and DNMT3B and subsequently led

96 Transcriptional repression of Dnmt1 by REMOTE-control was potent enough to cause embry

97 In contrast, the increase in DNMT1 by TGF-beta1 was not dependent on new protein synt

98 Degradation of Dnmt1 by the histone deacetylase inhibitor suberoylanili

99 ainst the maintenance DNA methyltransferase, DNMT1, by coupling Asymmetrical Flow Field-Flow Fraction

100 Deficiency of Dnmt1 causes severe intestinal abnormalities in neonates

101 fore showing a lower global DNA methylation (DNMT1(-/-) cells), were compared to HCT116 wildtype cell

102 Ultimately, cell death is elevated in the dnmt1(-/-) CMZ, but in a p53-independent manner.

103 e demonstrate increased transposition in the dnmt1(-/-) CMZ.

104 Functionally, KIT and DNMT1 co-expression promotes, whereas dual inactivation

105 how that endogenous DNA methyltransferase 1 (DNMT1) co-purifies with inhibitory ncRNAs.

106 n enter cancer cells with over-expression of DNMT1, colocalize with DNMT1 inside the nuclei, and inhi

107 he epigenetic action of CLO with that of the DNMT1 competitive inhibitor N-phthalyl-l-tryptophan (RG1

108 genesis by regulating the formation of STAT3-DNMT1 complex.

109 We generated Dnmt1 conditional knockout mice (Dnmt1(Deltaalb) ) by cr

110 DNMT1 contains, in addition to a C-terminal methyltransf

111 injury-induced pain hypersensitivities, DRG DNMT1 contributes to neuropathic pain genesis partially

112 In Dnmt1-deficient mice, optical projection tomography and

113 Acute Dnmt1 deletion elicits dramatic hypomethylation and geno

114 mammary tumours, and mammary gland-specific DNMT1 deletion protects mice from mammary tumorigenesis

115 sion of four genes, DNA methyltransferase 1 (DNMT1), delta-opioid receptor (OPRD1), cannabinoid recep

116 e generated Dnmt1 conditional knockout mice (Dnmt1(Deltaalb) ) by crossing Dnmt1(fl/fl) with albumin-

117 in postnatal liver growth and regeneration; Dnmt1(Deltaalb) mice provide a unique experimental model

118 The Dnmt1(Deltaalb) phenotype was assessed by histology, con

119 Understanding the TS of DNMT1 demonstrates the possibility of using similar anal

120 The finding of distinct roles of DNMT1-dependent and -independent methylation patterns in

121 ction and induces atherosclerosis in an mTOR/DNMT1-dependent manner.

122 DNMT1 expression increases, whereas targeted DNMT1 depletion abrogates KIT signaling cascade through

123 Noncytotoxic DNMT1 depletion was confirmed by serial BM gamma-H2AX (D

124 Upon DNMT1 depletion, OIS cells transition to a 3D genome con

125 cytotoxicity and increase exposure time for DNMT1 depletion, to safely and effectively circumvent mu

126 ure, which is required for S-phase-dependent DNMT1 depletion.

127 As expected with non-cytotoxic DNMT1-depletion, platelets increased and neutrophils con

128 DNMT1 (DNA methyltransferase 1) is responsible for propa

129 for 11 SNPs within DNA methyltransferase 1 (DNMT1), DNA methyltransferase 3 Beta (DNMT3B), Tet methy

130 et single (Mecp2) as well as multiple genes (Dnmt1, Dnmt3a and Dnmt3b) in the adult mouse brain in vi

131 In contrast to Dnmt1, Dnmt3a deficiency did not significantly alter lev

132 genetic targeting of DNA methyltransferases (Dnmt1, Dnmt3a, and Dnmt3b) and Ten-eleven translocation

133 use models, including DNA methyltransferase (Dnmt1, Dnmt3a, and Dnmt3b) knockout mice, optical projec

134 In podocytes, loss of Dnmt1, Dnmt3a, Dnmt3b, or Tet2 did not lead to functiona

135 genetic regulators (e.g., Foxo3, Plk1, Mycn, Dnmt1, Dnmt3b, and Tet3).

136 that miR-342 regulates DNMT1 expression but DNMT1 does not affect the EVL expression in these cells.

137 We further confirm the crucial role of Dnmt1 during crypt development using the in vitro organo

138 tion of MBD3 and MBD2 would co-localize with DNMT1 during DNA maintenance methylation, providing a pr

139 These patterns are faithfully maintained by DNMT1 during DNA replication to ensure epigenetic inheri

140 osphorylation of Ser143 (pSer143) stabilizes DNMT1 during DNA replication.

141 4B) through promoter demethylation; in turn, DNMT1 dysfunction impairs KIT kinase signaling.

142 with preferred DNA substrates revealing that DNMT1 employs flanking sequence-dependent base flipping

143 , such as miR-155-5p, leads to inhibition of DNMT1 enzyme activity.

144 Kras(G12D)-induced senescence by regulating Dnmt1 expression and consequently genome-wide levels of

145 The Myt1(+)Neurog3(+) cells displayed higher Dnmt1 expression and enhancer methylation at Arx, an alp

146 xpression, suggesting that miR-342 regulates DNMT1 expression but DNMT1 does not affect the EVL expre

147 ding that RAS/MEK/ERK pathway activation and DNMT1 expression correlated with cytotoxic activity.

148 Peripheral nerve injury upregulated DNMT1 expression in the injured DRG through the transcri

149 a positive regulatory loop, in which ectopic DNMT1 expression increases, whereas targeted DNMT1 deple

150 This study demonstrates that Dnmt1 expression is correlated with proliferation in mel

151 Furthermore, we find that DNMT1 expression is elevated in mammary tumours, and mam

152 Dnmt1 expression was analyzed in primary melanocytes, me

153 Dnmt1 expression was correlated with Ki-67 expression (S

154 Dnmt1 expression was increased incrementally from nevi [

155 oxycytidine as well as by down-modulation of Dnmt1 expression, further supporting the role of DNA met

156 ed form of STAT3 transcriptionally activated DNMT1 expression.

157 In contrast, Six2 (Cre) Dnmt1 (f/f) mice died within 24 hours of birth, from a s

158 knockout mice (Dnmt1(Deltaalb) ) by crossing Dnmt1(fl/fl) with albumin-cyclization recombination tran

159 gene-body hypermethylation, dissociation of DNMT1 from the promoter, and VAV1 expression via SMAD4 a

160 gether our data identify a critical role for dnmt1 function in RSC maintenance in the vertebrate eye.

161 Loss of dnmt1 function results in gene misregulation and cell de

162 h the epigenetic machinery especially UHRF1, DNMT1, G9a and the transcription factor Snail1.

163 n mouse embryonic stem cells that endogenous Dnmt1 gene transcription could be up- or downregulated i

164 the hereditary motor and sensory neuropathy DNMT1 gene, highlighting the genetic heterogeneity of sp

165 tein-triggered transcriptional activation of Dnmt1 gene.

166 uence (RFTS) of the DNA methyltransferase 1 (DNMT1) gene.

167 omains 1 (uhrf1) or DNA methyltransferase 1 (dnmt1) genes exhibit a robust interferon induction chara

168 direction of these associations depended on DNMT1 genotypes.

169 Our data show that DNMT1 has imprecise maintenance activity and possibly po

170 MCT-RVfib manifest a DNMT1-HIF-1alpha-PDK-mediated, chamber-specific, metabol

171 ow that the flanking sequence preferences of DNMT1 highly correlate with genomic methylation in human

172 f pluripotent genes in the first step, while Dnmt1 impedes 2-cell-embryo-specific gene activation in

173 that interact with the DNA methyltransferase DNMT1 in a colon cancer cell line, HCT116.

174 cyclization recombination-driven deletion of Dnmt1 in adult quiescent hepatocytes did not affect live

175 side the nuclei, and inhibit the activity of DNMT1 in cells.

176 Pharmacologic or genetic targeting of DNMT1 in CSCs reduced their self-renewal and in vivo tum

177 Consistently, efficient knockdown of Dnmt1 in cultured hepatic progenitor cells caused severe

178 Furthermore, genetic deletion of Dnmt1 in HSPCs activated Gata1 expression and depleted H

179 ustrate a clear differential requirement for Dnmt1 in immature versus mature organoids.

180 se results demonstrate an essential role for Dnmt1 in maintaining genomic stability during intestinal

181 r, our studies uncover an essential role for DNMT1 in MaSC and CSC maintenance and identify DNMT1-ISL

182 Ablation of Dnmt1 in mouse embryos causes death at the post-gastrula

183 Deletion of Dnmt1 in nephron progenitor cells (in contrast to deleti

184 nd the H3K9me3Ub affects the localization of DNMT1 in stem cells and profoundly impairs the global DN

185 entify functional cross-talk between KIT and DNMT1 in the development of drug resistance, implying th

186 We investigated the role of DNMT1 in the regulation of postnatal liver histogenesis

187 that CDK4 interacted with and phosphorylated DNMT1 in vitro, suggesting that CDK activity is required

188 ased expression of the DNA methyltransferase DNMT1 in WDLS/DDLS.

189 STAT3 that targeted DNA methyltransferase 1 (DNMT1) in a sequential manner.

190 downregulation of the DNA methyltransferase DNMT1 induced by the brain microenvironment-derived clus

191 te loss of the maintenance methyltransferase Dnmt1 induces widespread DNA demethylation and transcrip

192 DS AND To pharmacologically re-induce HbF by DNMT1 inhibition, this first-in-human clinical trial (NC

193 dy indicates that 5-aza may function through Dnmt1 inhibition.

194 Administering Dnmt1 inhibitor to control neonates resulted in FAE-like

195 hown that 5-Aza-2'deoxycytidine (5-AzadC), a DNMT1 inhibitor, induces re-expression of tumor suppress

196 ncentrations of the DNA methyltransferase 1 (DNMT1) inhibitor decitabine produce p53-independent cell

197 thyltransferase and DNA-methyltransferase 1 (DNMT1) inhibitor in human HCC cells and their crosstalk

198 doxycycline-mediated repression of exogenous DNMT1* initiates rapid, global loss of DNA methylation,

199 th over-expression of DNMT1, colocalize with DNMT1 inside the nuclei, and inhibit the activity of DNM

200 y Microprocessor component DROSHA as a novel DNMT1-interactor.

201 The proposed catalytic mechanism of DNMT1 involves nucleophilic attack of Cys(1226) to cytos

202 transcriptional activation in vivo, and that Dnmt1 is a critical regulator of postnatal epigenetic ch

203 Because 5-methylcytosine methyltransferase DNMT1 is also a potential target of miR-342, we inhibite

204 DNA methylation maintenance by DNMT1 is an essential process in mammals but molecular m

205 Dnmt1 is critical for immediate postnatal intestinal dev

206 Here, we report that Dnmt1 is crucial during perinatal intestinal development

207 facilitating proper differentiation, whereas DNMT1 is essential for maintaining global methylation le

208 Here we show that DNMT1 is indispensable for MaSC maintenance.

209 We demonstrated that DNMT1 is not localized within mitochondria, but it is as

210 tissues, we found that the overexpression of DNMT1 is positively correlated with the upregulation of

211 DNA methyltransferase 1 (DNMT1) is an essential regulator maintaining both epigen

212 malian DNA (cytosine-5) methyltransferase 1 (DNMT1) is essential for maintenance methylation.

213 The maintenance methyltransferase, dnmt1, is expressed within the CMZ.

214 protein, the essential targeting factor for DNMT1, is reduced upon transition to 2i, and so is recru

215 MT1 in MaSC and CSC maintenance and identify DNMT1-ISL1 axis as a potential therapeutic target for br

216 d expression of the DNA methyltransferase 1 (DNMT1) isoform.

217 ts are phenocopied by mutation of dnmt1, and Dnmt1 knockdown in uhrf1 mutants enhances their small li

218 Further, DNMT1 knockdown sensitized PKC412(R) cells to PKC412; co

219 The impact of Dnmt1 knockdown was also analyzed in hepatic progenitor

220 n data from wild-type, DNMT3A, DNMT3A/3B and DNMT1 knockout human embryonic stem cells and observed a

221 ed with marked downregulation of miR-221 and DNMT1, leading to restored p27kip1 and p15ink4b tumor su

222 Mechanistically, CSC expressed higher DNMT1 levels than non-CSC.

223 UV39 histone methyltransferase, DIM-5, and a DNMT1-like cytosine methyltransferase, DIM-2.

224 Dnmt1 loss in postnatal hepatocytes caused global hypome

225 omised in vivo in a mouse model of transient DNMT1 loss in the preimplantation embryo.

226 The DNA methyltransferase Dnmt1 maintains DNA methylation patterns and genomic sta

227 Human DNA methyltransferase 1 (DNMT1) maintains the epigenetic state of DNA by replicat

228 ropathic pain, our findings suggest that DRG DNMT1 may be a potential target for neuropathic pain man

229 ts strong expression in metastatic melanoma, Dnmt1 may be a promising target for combined epigenetic

230 The regulatory domains of DNMT1 mediate a network of protein-protein and protein-D

231 tion (H3K27me3) and DNA methyltransferase 1 (DNMT1)-mediated DNA methylation repress the tumour produ

232 ate interplay between CpG-flanking sequence, DNMT1-mediated base flipping and the dynamic landscape o

233 nal lineage genes and transposable elements, DNMT1-mediated cytosine methylation is essential for kid

234 results establish the indispensable role of DNMT1-mediated epigenetic regulation in postnatal liver

235 ay through which H3K9me3 directly reinforces DNMT1-mediated maintenance DNA methylation.

236 processed by Microprocessor and can inhibit DNMT1-mediated methylation in vitro.

237 DNMT1-mediated methyltransferase activity is also reduce

238 We documented impaired activity of purified DNMT1 mutant proteins, which in fibroblasts results in i

239 RNA-seq analysis of uhrf1 and dnmt1 mutants revealed widespread induction of Class I r

240 drial involvement, we studied the effects of DNMT1 mutations in fibroblasts from four ADCA-DN and two

241 hogenic mechanism for DNA methyltransferase (DNMT1) mutations.

242 thylated DNA duplex, the native substrate of DNMT1, off the protein on sub-micromolar scale, leading

243 ndividual and combined inhibition of G9a and DNMT1 on HCC cell growth by pharmacological and genetic

244 ion of DNA (cytosine-5)-methyltransferase 1 (DNMT1)-only cells produces a heterogeneous methylome, wh

245 sfection, reduced DNMT3a expression, but not DNMT1 or -3b, disrupted sarcomere assembly and decreased

246 disrupted two major DNA methyltransferases, Dnmt1 or Dnmt3a, in fetal and adult intestine.

247 ithelium is lethal, while deletion of either Dnmt1 or Dnmt3b has no effect on survival.

248 ic BP overall, associations within strata of DNMT1 or DNMT3B were observed, and the magnitude and the

249 While the deregulated activation of DNMT1 or KIT has been implicated in lung cancer pathogen

250 ro, which were attributed to the hyperactive DNMT1 or KIT, because inactivation of KIT or DNMT1 recip

251 ors promoted alpha cell specification, while Dnmt1 overexpression or Arx enhancer hypermethylation fa

252 Our results define the RAS/MEK/DNMT1 pathway as a determinant of sensitivity to DNA met

253 Independent G9a and DNMT1 pharmacological targeting synergistically inhibite

254 Here we show that Dnmt1 preserves DNA methylation through stage I at ICRs

255 ins silencing of IAPs, but in the absence of DNMT1, prolonged binding of NP95 to hemimethylated DNA t

256 transcriptional activators to the endogenous Dnmt1 promoter resulted in robust upregulation of this h

257 In addition, knockdown of DNMT3A or DNMT1 protected neurons against mutant Htt-induced toxic

258 This dose decreased DNMT1 protein in peripheral blood mononuclear cells by >

259 Moreover, we show that mutant DNMT1 proteins translocate to the cytoplasm and are pron

260 DNMT1 or KIT, because inactivation of KIT or DNMT1 reciprocally blocked decitabine(R) or PKC412(R) ce

261 p disrupts its binding to the ICR of H19 and Dnmt1 recruitment.

262 Our results suggest mutations in DNMT1 result in imbalanced protein homeostasis through a

263 n contrast to findings in mouse, deletion of DNMT1 resulted in rapid cell death in human ESCs.

264 e systematically analyzed the specificity of DNMT1, revealing a pronounced influence of the DNA seque

265 We propose that DNMT1 RFTS mutations deregulate metabolism lowering ATP

266 Here, we demonstrate that zebrafish dnmt1(s872) mutants possess severe defects in RSC mainte

267 h DNA methylation inhibitors (Azacytidine or Dnmt1-siRNA), prevented Mfn2 and Mlh1 hypermethylation,

268 rivatives as potent inhibitors of DNMT3A and DNMT1, some showing certain isoform selectivity.

269 of 14-3-3 in NIH3T3 cells led to decrease in DNMT1 specific activity resulting in hypomethylation of

270 Here, we determined that DNMT1-specific inhibition in arterial wall ameliorates t

271 secreted from myoepithelial cells influences DNMT1 stability, induces the expression of VEGFR2 in end

272 We determined DNMT1 structures in complex with preferred DNA substrate

273 Transient DNMT1 suppression revealed gDMD-like sequences requiring

274 s the mechanistic understanding of the UHRF1/DNMT1 tandem and the development of assays for the ident

275 lation studies, we identify ISL1 as a direct DNMT1 target, hypermethylated and downregulated in mamma

276 ampal expression of DNA methyltransferase 1 (Dnmt1) that maintains the imprinting of Dio3 and Igf2 du

277 ora kinase B, the centromeric cohesin ESCO2, DNMT1, the ubiquitin-ligase (DZIP3) and the histone meth

278 the previously determined structure of mouse DNMT1, this study also reveals a number of distinct stru

279 HRF1, and thus the probability of recruiting DNMT1 to faithfully duplicate the DNA methylation profil

280 ced acetylated form of STAT3 interacted with DNMT1 to form a transcription factor complex that bound

281 ing PHD and RING finger domains, 1) recruits DNMT1 to hemimethylated DNA during replication and is es

282 repress stem/pluripotent genes by recruiting DNMT1 to methylate some promoters and suppress TICs.

283 sorder, show an ~2-fold increased binding of DNMT1 to psychiatric candidate promoters (glutamic acid

284 nding RING E3 ubiquitin ligase that recruits DNMT1 to sites of newly replicated DNA through ubiquityl

285 ilitates binding of DNA methyltransferase 1 (Dnmt1) to DNA substrates, including the ICRs of the impr

286 s, but a reduction of KIT expression ablates DNMT1 transcription by STAT3 pathway leading to in-paral

287 inase-3beta, which resulted in inhibition of DNMT1 ubiquitination and proteosomal degradation.

288 an target of rapamycin (mTOR) suppressed the DNMT1 up-regulation both in vitro and in vivo.

289 SCD was safe in this study and, by targeting DNMT1, upregulated HbF in RBCs.

290 All 5-CSRTT deficits were associated with DNMT1 upregulation, whereas impulsive behavior could be

291 In BRAF-mutant melanoma, Dnmt1 was down-regulated during response to B-Raf and ME

292 DAXX or DNMT1 was necessary to inhibit methylation of CpGs withi

293 ary expression of the DNA methylation enzyme DNMT1 was stable in CR mice but increased over time in o

294 we inhibited miR-342 in MCF7 cells and found DNMT1 was up-regulated with no change in EVL expression,

295 A demethylation induced by acute deletion of Dnmt1, we saw an increase in sense transcription at TEs,

296 Five novel mutations of DNMT1 were discovered; p.C353F, p.T481P, p.P491L, p.Y524

297 ses, G9a/GLP, and the DNA methyltransferase, DNMT1, which both control keratinocyte senescence.

298 ere we report the crystal structure of human DNMT1 with all the structural domains (hDNMT1, residues

299 Conclusion: Combined targeting of G9a/DNMT1 with compounds such as CM-272 is a promising strat

300 o identify minimal doses active in depleting DNMT1 without cytotoxicity.