ライフサイエンスコーパス: DTT

1 DTT activity was more strongly associated with emergency

2 DTT analysis in LMTS shows a marginally significant decr

3 DTT enhancement of the NMDAR response was dependent on C

4 DTT is feasible in term newborns and may help to charact

5 DTT likely functions in a ligand substitution reaction t

6 DTT prevents oxidation of BH4 in both isoforms, but in n

7 DTT was performed to segment bilateral pyramidal tracts

8 DTT, in contrast, induces a conversion to the extended f

9 stitution reaction that generates a [2Fe-2S]-DTT species, which can transfer the cluster to either la

10 e PAM-ABP/siRNA polyplex was released by 5mM DTT and heparin.

11 e and collect the sandwich structures, and a DTT solution at elevated temperature is used to release

12 IPK1 E82C/S142C exhibited a DTT-sensitive 5-fold increase in kcat for 3,4,5,6-inosit

13 OxyR and its target genes katG and oxyS in a DTT-reversible manner.

14 xposed to thapsigargin, A23187, brefeldin A, DTT, geldanamycin, or bortezomib manifested reduced acti

15 bonds to reduction by extracellularly added DTT and Triton X-114 detergent phase partitioning.

16 h the DS Env were not infectious, even after DTT treatment.

17 ) treatment; nine Cs could be modified after DTT treatment.

18 atment, and eight Cs could be modified after DTT treatment.

19 l eight Cs could be modified before or after DTT treatment.

20 ponding to OA-NO2; however, a reducing agent DTT (50 mm) or La(3+) (50 mum) completely abolished OA-N

21 Reducing agent DTT and reactive oxygen species (ROS) scavenger tempol,

22 plication of GSH or the thiol-reducing agent DTT can rescue the root phenotype of miao, demonstrating

23 tely blocked by addition of a reducing agent DTT or N-acetyl-L-cysteine, showing that process of oxid

24 ersed in the presence of the reducing agent, DTT, thus suggesting that S-nitrosylation of thiolate-zi

25 Using NEM for thiol alkylation, DTT for disulfide reduction, and mBBr for labeling the r

26 om an urban site were analyzed, and although DTT oxidation was significantly correlated with H2O2 gen

27 rB2) and MsrB3 (hMsrB3) showed that although DTT can function in vitro as the reducing agent, Trx wor

28 ential of two buffer additives (Tween 20 and DTT) to improve the solubility of proteins from shrimp s

29 lyso-PAF acetyltransferase (lyso-PAF-AT) and DTT-insensitive CDP-choline 1-alkyl-2-acetyl-sn-glycerol

30 the dependence of looping on salt, ATP, and DTT using full-length E2 and an E2 protein containing on

31 anisms for Pho reduction via PhoR(C303A) and DTT are different.

32 oreover, using enzymatic deglycosylation and DTT derivatization combined with mass spectrometry techn

33 -Ras-GppNHp/NOR1A complex, and PEG, DTE, and DTT stabilize the anticatalytic conformation observed in

34 study, we aim to evaluate the use of DTI and DTT as a predictive model in the field of epilepsy, spec

35 The SDS- and DTT-resistant oligomerization was studied further as it

36 were able to complement the temperature and DTT sensitive phenotype, although a truncated eroA, miss

37 ycin, brefeldin A (brefA), thapsigargin, and DTT that lead to accumulation of unfolded proteins withi

38 ds, including l-homocysteine thiolactone and DTT, induced VEGF expression 7.9- and 8.8-fold.

39 tically favorable buffer containing zinc and DTT reduced the interchain disulfide bond releasing and

40 separate environmental conditions (zinc and DTT treatment) yielded transcription networks consistent

41 s were incubated with cell stressors such as DTT, IFN, and adherent-invasive E coli or control agents

42 rement is based on the dithiothreitol assay (DTT assay), uses colorimetric detection, and can be comp

43 ant enzymes, six Cs could be modified before DTT treatment, and eight Cs could be modified after DTT

44 = 0.91), no correlation was observed between DTT oxidation and (*)OH formation.

45 Our results show that measuring both DTT consumption and ROS generation in the DTT assay is i

46 Partial rescue of activity for aged 20S by DTT implies oxidation of functionally significant cystei

47 LA(2)beta inactivation that is attenuated by DTT or ATP.

48 P, and inhibition of autophosphorylation by DTT.

49 and brefA, whereas acute ER stress caused by DTT and thapsigargin leads to rapid and specific degrada

50 diminished under mild reducing conditions by DTT and enhanced by H(2)O(2) oxidation.

51 tate and was blocked rather than enhanced by DTT.

52 tion and remained sensitive to inhibition by DTT, suggesting that the mechanisms for Pho reduction vi

53 over, H(2)O(2) reversed ADAM17 inhibition by DTT.

54 e-out patches, this crosslink was reduced by DTT and reoxidized by a membrane-impermeant bis-quaterna

55 in an oxidized form and was not reducible by DTT.

56 bonds and measure their rate of reduction by DTT.

57 ed this activity, which could be reversed by DTT.

58 for UA, alternative antioxidants (vitamin C, DTT, and N-acetylcysteine) also enhanced the chemotactic

59 DEP was highly redox-active, causing DTT to decay at a rate of 23-61 pmol min(-1) mug(-1) of

60 inear optics and dye-sensitized solar cells, DTT polymers in light-emitting diodes, organic field-eff

61 redoxin reductase/thioredoxin) and chemical (DTT) turnover.

62 ion under three different stress conditions (DTT, H2O2, and nitrogen starvation) using the GFP-tagged

63 because oxidant-treated iPLA(2)beta contains DTT-reducible oligomers, and oligomerization occurs with

64 Dithiothreitol (DTT) is the standard reagent for reducing disulfide bond

65 Dithiothreitol (DTT) was used in all solutions from the beginning, consi

66 A dithiothreitol (DTT) assay was used to measure the ROS-generation potent

67 Adding dithiothreitol (DTT) is the standard method for liquefaction prior to pr

68 versed by the reducing agent dithiothreitol (DTT) and the specific deglutathionylation reagent glutar

69 ted using the reducing agent dithiothreitol (DTT) in an assay that allowed the time for deoxygenation

70 nversely, the reducing agent dithiothreitol (DTT) selectively enhanced NMDAR response to a greater ex

71 locked by the reducing agent dithiothreitol (DTT).

72 tion with the reducing agent dithiothreitol (DTT, 1 mM) prevented drug-induced inhibition of channel

73 by the thiol-reducing agent dithiothreitol (DTT, 10 mm) and inhibited by the oxidizing agent diamide

74 The thiol reducing agent dithiothreitol (DTT, 5.0 mm) both prevented and reversed HNO action, con

75 xposure to a reducing agent, dithiothreitol (DTT).

76 cts of three reducing agents-dithiothreitol (DTT), beta-mercaptoethanol (beta-MCE), and tris(2-carbox

77 river of HOOH production and dithiothreitol (DTT) loss in ambient PM extracts.

78 e relies on formaldehyde and dithiothreitol (DTT), but these active chemicals may introduce artifacts

79 esence of suspended NP's and dithiothreitol (DTT).

80 A semi-automated dithiothreitol (DTT) analytical system was used to measure daily average

81 Cs could be modified before dithiothreitol (DTT) treatment; nine Cs could be modified after DTT trea

82 equires the presence of both dithiothreitol (DTT) and 4 equiv of Fe(II) for maximum activity.

83 potential (OP) determined by dithiothreitol (DTT) consumption and intracellular reactive oxygen and n

84 ing environment generated by dithiothreitol (DTT) in vivo inhibited Pho induction in a PhoR-dependent

85 nlabeled Fdx is catalyzed by dithiothreitol (DTT), a result that was confirmed by mass spectrometry.

86 cs of disulfide reduction by dithiothreitol (DTT).

87 can be partially reversed by dithiothreitol (DTT).

88 erivatives using a cell-free dithiothreitol (DTT) assay under simulated physiological conditions (37

89 ulin disulfide reductions in dithiothreitol (DTT) over a range of heater temperatures (22-70 degrees

90 h postcolumn introduction of dithiothreitol (DTT) and ammonium hydroxide, each disulfide-containing p

91 stable isotopic variants of dithiothreitol (DTT) and MS analysis.

92 The relative efficacy of dithiothreitol (DTT) and tris(2-carboxyethyl)phosphine (TCEP) for reduci

93 ogical pH in the presence of dithiothreitol (DTT), and shows typical half-times of equilibration in t

94 n assayed in the presence of dithiothreitol (DTT), the inhibitory effect was drastically reduced.

95 n the presence or absence of dithiothreitol (DTT).

96 er dithioerythritol (DTE) or dithiothreitol (DTT) soaked into H-Ras-GppNHp crystals in the presence o

97 rom the presence of residual dithiothreitol (DTT), a reagent that reduces cell viability and interfer

98 ation of the model substrate dithiothreitol (DTT).

99 matically measured using the dithiothreitol (DTT) assay (OP(DTT)).

100 Using the dithiothreitol (DTT) assay as a measure of OP, a combination of field ob

101 ived SOA, as measured by the dithiothreitol (DTT) assay, suggested the presence of oxidizing moieties

102 measurement is based on the dithiothreitol (DTT) assay, where, after being oxidized by PM, the remai

103 ngine was examined using the dithiothreitol (DTT) assay.

104 ter (PM) was measured by the dithiothreitol (DTT) assay.

105 rticulate matter (PM) in the dithiothreitol (DTT) assay.

106 articles was measured by the dithiothreitol (DTT) assay.

107 insignificant impact on the dithiothreitol (DTT) oxidase activity of ALR.

108 imers that were sensitive to dithiothreitol (DTT), dependent on the Mip domain and on at least one cy

109 hat these are susceptible to dithiothreitol (DTT).

110 free drug was verified using dithiothreitol (DTT) and glutathione (GSH) as liberating agents.

111 hemical derivatization using dithiothreitol (DTT) of the phospho-serine/threonine-containing peptides

112 ntrated reducing agent, viz. dithiothreitol (DTT) or tris(2-carboxyethyl)phosphine (TCEP), was added

113 when cells were treated with dithiothreitol (DTT) but not Mn(2+).

114 stability when treated with dithiothreitol (DTT) compared with monothiol analogues.

115 f purified portal rings with dithiothreitol (DTT) resulted in the disruption of the rings, suggesting

116 Treatment of sera with dithiothreitol (DTT) showed that the majority of remaining lymphocytotox

117 iked sample was treated with dithiothreitol (DTT) to convert disulfide-bonded glutathione to GSH.

118 ld nanoparticle surface with dithiothreitol (DTT), which simplifies the assay and increases its quant

119 do esters, when treated with dithiothreitol (DTT)/diisopropylethylamine (DIPEA), undergo both azido g

120 Pretreatment of IPAs with dithiothreitol (DTT, 1 mm), proposed to promote the conversion of mitoch

121 M HEPES buffer, pH 7.5, 0.1% dithiothreitol [DTT]).

122 NaCl, 0.5% NP-40, and 10 mM dithiothreitol [DTT].

123 D was found to be induced by dithiothrietol (DTT), through the MisR/S regulatory system.

124 mall molecules and proteins faster than does DTT.

125 ends of the narrow optical gap copolymer DPP-DTT with PC70BM show two distinct spectrally flat region

126 levels of several inflammatory genes in EDTA/DTT when compared to enzymatically released cells.

127 el (i.e. terminal ileum) biopsies using EDTA/DTT and enzymatic release followed by magnetic bead sort

128 Elimination of this bond, by either DTT-mediated reduction or mutagenesis, enhances gating e

129 hylrhodamine-labeled rPfP2 protein exhibited DTT- and SDS-resistant oligomerization when treated with

130 /E229C)(+), only responded to 5-HT following DTT treatment in both homomeric and heteromeric receptor

131 column, and both fractions were analyzed for DTT activity and water-soluble metals.

132 knockdown in NRVMs increased cell death from DTT-mediated reductive ER stress, but not from nonreduct

133 nce of various reductants and oxidants (GSH, DTT, cysteine, O2, hydrogen peroxide, and superoxide), w

134 n fine structure spectroscopy of the MerB/Hg/DTT complex have shown that the ligands to the mercuric

135 To determine if DTT treatment homogenizes the bacterial distribution wit

136 rsions of MerA and a nonphysiological Hg(II)-DTT-MerB complex qualitatively support a pathway for dir

137 Importantly, DTT abrogates NS5A-NS5A interactions but does not affect

138 as evident from a substantial attenuation in DTT response after passing PM extracts through the C-18

139 results showed no significant differences in DTT consumption rate measured by the two methods.

140 Fe is mostly inactive in DTT oxidation, but shows synergistic effect in (*)OH for

141 n HU and a tolerance to mutation at Lys63 in DTT.

142 Cu(II), a dominant metal in DTT oxidation, contributes almost negligibly to the ROS

143 nd their mixtures show different patterns in DTT oxidation versus ROS generation.

144 /MS analyses of tryptic digests and included DTT-reversible events, e.g., formation of disulfide bond

145 icles (LDGV) exhibited the highest intrinsic DTT activity, followed by biomass burning (BURN) and hea

146 donor-labeled (H)FTACs and acceptor-labeled DTT upon addition of lipid vesicles indicates that the p

147 presence of oxygen and a reducing agent like DTT.

148 posing the oocytes to a competing thiol like DTT (dithiothreitol) and 2-ME (2-mercaptoethanol).

149 ospheric dilution can transform the OP(mass)(DTT) of the water-soluble fraction of wood smoke within

150 during the fire seasons in Greece, OP(mass)(DTT) was observed to increase by a factor of 2.1 +/- 0.9

151 aging transforms the intrinsic OP (OP(mass)(DTT)) of BBOA.

152 d cells imaged in buffer at pH 8.5 with 1 mM DTT, indicating that light chemical fixation does not de

153 uble cell fractions in the presence of 10 mM DTT, shows UDP-N-acetylglucosamine 6-dehydrogenase activ

154 gnetic resonance) and is based on monitoring DTT-dependent (13)C chemical shift changes of the human

155 interaction of surfactants with a model MP, DTT, using a variety of spectroscopic techniques.

156 The utility of this new DTT-based system is demonstrated by detecting a mock mRN

157 ase and 0.07 (95% CI: 0.00, 0.44) per 1-nmol DTT/min/m3 oxidative potential increase].

158 dation of BH4 in both isoforms, but in nNOS, DTT also inhibits oxidation of two key cysteines in nNOS

159 yields active mutant enzymes that exhibit no DTT-irreversible oxidative inactivation.

160 At neutral pH, however, >99% of DTT thiol groups are protonated and thus unreactive.

161 a more careful comparison of the ability of DTT and Trx to function as reducing agents with the vari

162 ependent and irreversible (in the absence of DTT), ultimately resulting in protein denaturation.

163 ces superoxide in the presence or absence of DTT.

164 nate (p-XSC) was reversed by the addition of DTT; this suggests the formation of DTT-reducible seleni

165 Burkholderia multivorans between aliquots of DTT-treated sputum samples with and without a mechanical

166 h concentrations along with large amounts of DTT.

167 increases linearly with the concentration of DTT and exponentially with mechanical tension along the

168 s linearly dependent on the concentration of DTT, it is exponentially dependent on the applied force,

169 by adjusting the relative concentrations of DTT and Hb.

170 The linear dependence of DTT's enthalpy of unfolding on the surfactant concentrat

171 , presumably because of slow dissociation of DTT-Cu complexes.

172 n, several peptides within the CH2 domain of DTT-IYG showed differential deuterium uptake in the pept

173 ition of DTT; this suggests the formation of DTT-reducible selenium-sulfur bonds between selenocyanat

174 pical BH4 radical in a manner independent of DTT.

175 Site-selective labeling of DTT with fluorescent dyes indicates that the surfactants

176 tential and to estimate historical levels of DTT activity for use in an epidemiologic analysis for th

177 d thioredoxin (Trx), although high levels of DTT can be used as the reductant in vitro.

178 In the presence of DTT and the NO inhibitor N(omega)-nitro-L-arginine methy

179 Unexpectedly, in the presence of DTT, doxorubicinol enhanced the rate of Ca(2+) uptake by

180 The role of DTT is also different.

181 The specificity of DTT chemical derivatization was evaluated separately und

182 surfactants does not alter the structure of DTT.

183 values that are ~1 unit lower than those of DTT and forms a disulfide with a similar E degrees ' val

184 This direct validation of DTT measurements by histological methods in monkeys was

185 nection length in the primate brain based on DTT imaging.

186 Cys651-sulfenic acid formation could be one DTT-reversible inactivation event because Cys651 modific

187 nces in aliquots that were subjected to only DTT treatment and those of the aliquots which included a

188 OP(DTT) was measured for 196 d (mean=0.32 nmol/min/m(3), in

189 Lag 0-2 OP(DTT) remained a significant predictor of asthma and isch

190 Lag 0-2 OP(DTT) was associated with ED visits for multiple cardiore

191 Lag 0-2 OP(DTT) was associated with ED visits for respiratory disea

192 Although OP(DTT) per mass (toxicity) was highest for ultrafine parti

193 OP(AA) and OP(DTT) were primarily associated with traffic-related comp

194 red using the dithiothreitol (DTT) assay (OP(DTT)).

195 for OP(AA) ( R(CV)(2) = 0.48) followed by OP(DTT) ( R(CV)(2) = 0.32) and OP(GSH) ( R(CV)(2) = 0.22).

196 Same-day OP(DTT) was not associated with ED visits for any outcome.

197 average (lag 0-2) of OP(DTT) or same-day OP(DTT).

198 and OP(AA)) and dithiothreitol depletion (OP(DTT)).

199 ng winter, model performance was best for OP(DTT) ( R(CV)(2) = 0.55) followed by OP(GSH) ( R(CV)(2) =

200 1.05 per interquartile range increase in OP(DTT)), asthma (RR=1.12, 95% CI: 1.03, 1.22), and ischemi

201 the same, except that for water-insoluble OP(DTT) the compounds were absorbed on surfaces of soot and

202 In contrast, water-insoluble OP(DTT) was bimodal, with both fine and coarse modes.

203 mes, providing support for the utility of OP(DTT) as a measure of fine particle toxicity.

204 Contrasts in the phases of these forms of OP(DTT) deposited in the respiratory system may have differ

205 ither the 3-d moving average (lag 0-2) of OP(DTT) or same-day OP(DTT).

206 ing functional groups, and enhancement of OP(DTT) values.

207 un to estimate the health associations of OP(DTT) while controlling for other pollutants.

208 tem was used to measure daily average OP (OP(DTT)) in water-soluble fine PM at a central monitor site

209 ions of water-insoluble and water-soluble OP(DTT) (dithiothreitol assay, measure of oxidative potenti

210 Water-soluble OP(DTT) was unimodal, peaked near 1-2.5 mum due to contribu

211 methylglyoxal and NADH, NADPH, F 420H 2, or DTT to a M. jannaschii cell extract stimulated the produ

212 d with an anticatalytic conformation (DTE or DTT).

213 aining chloride salts and no formaldehyde or DTT.

214 timulation of murine macrophages with LPS or DTT facilitated cell surface translocation of calreticul

215 ch were disrupted by the addition of 2-ME or DTT, which reduced the single disulfide bond found in Pr

216 (thioredoxin/thioredoxin reductase/NADPH or DTT), the enzyme inactivation results from its covalent

217 e coupled in good yields with either TCEP or DTT as the reductant, though some byproducts are observe

218 nd bovine MsrA efficiently use either Trx or DTT as reducing agents.

219 Msr reaction in the absence of either Trx or DTT.

220 ssed ER, treated acutely with tunicamycin or DTT, either is comparable to homeostasis or significantl

221 to ER stress caused by either tunicamycin or DTT.

222 The quinones, known to oxidize DTT in the efficiency order of PQ > 5H-1,4NQ > 1,2-NQ >

223 proteins under three external perturbations (DTT, H(2)O(2) and nitrogen starvation) and two genetic m

224 ffect transistors and organic photovoltaics, DTT self-assembly and templated assembly into thin films

225 ial, allowing selective detection of reduced DTT.

226 being oxidized by PM, the remaining reduced DTT is analyzed by the microfluidic sensor.

227 l groups in p65, whereas the thiol reductant DTT reversed the inhibiting effect of H2S on the p65 DNA

228 reduced and reactivated with the reductants DTT and gluthathione, whereas only the catalytic domain

229 ein treated with three different reductants (DTT, beta-mercaptoethanol, and TCEP).

230 The on-line sensor reported DTT consumption rates (oxidative activity) in good corre

231 ry cells from a solution containing residual DTT to a buffer solution.

232 ur acoustofluidic technique removes residual DTT generated in sputum liquefaction and facilitates imm

233 Aglycosylated constructs N297D/S298T (DTT)-K326I/A327Y/L328G (IYG) and N297D/S298A-IYG optimal

234 after dissolving in dithiothreitol solution (DTT 0.8M).

235 ned the effects of the reductive ER stressor DTT.

236 lipid bilayers rapidly (>30-fold faster than DTT), whereas the parent TCEP is impermeant.

237 CaMKII activity assays established that DTT increased CaMKII activity in CA1 cytosolic extracts

238 ified between injury groups, suggesting that DTT may provide more sensitive measures.

239 The DTT activation could be indicative of the presence of a

240 The DTT activities of water and methanol extracts were corre

241 The DTT assay response was significantly higher for the meth

242 The DTT-mediated growth in the NMDAR response was not observ

243 The DTT-phosphopeptides were enriched by covalent disulfide-

244 The DTT-treated fungal cells also showed evidence for the in

245 dox activity on their own as measured by the DTT assay, but they enhanced ROS generation catalyzed by

246 71 and C151 cysteine thiols, produced by the DTT-dependent reduction of their disulfide, are two addi

247 with one hydrophobic residue eliminated the DTT effect but with two hydrophobic residues made the pe

248 dy, we found that Cys(8) is required for the DTT-sensitive mono- and diubiquitination of Pex20.

249 the complex consist of both sulfurs from the DTT molecule and one cysteine ligand, C96, from the prot

250 ine the relationship between activity in the DTT assay and toxicology measurements across particles o

251 th DTT consumption and ROS generation in the DTT assay is important to incorporate the synergistic co

252 s continued assessment of the utility of the DTT activity assay as a measure of ROS-generating potent

253 The association of the DTT activity of water-soluble PM2.5 (WS_DTT) with these

254 ase with the depth of the sigma holes of the DTT anionophores.

255 brief summary of synthetic approaches to the DTT scaffold, chalcogen bonds are introduced as, togethe

256 Exposure of cells to DTT immediately before CO exposure also dramatically red

257 ide and enzymatic O-deglycosylation prior to DTT reaction.

258 vA is slightly down-regulated in response to DTT stress yet up-regulated in response to expression of

259 or ER-associated degradation in response to DTT-mediated ER stress.

260 These properties were sensitive to DTT, suggesting that avicins affect one or more critical

261 RN contributed the largest fraction to total DTT activity over the study period, followed by LDGV and

262 does not increase the turnover number toward DTT.

263 3 Tesla scanner for diffusion tract tracing (DTT) reconstruction of callosal bundles from different a

264 ing (DTI) and Diffusion Tensor Tractography (DTT).

265 nance imaging diffusion tensor tractography (DTT).

266 assay was validated against the traditional DTT assay using 13 extracted aerosol samples including u

267 s validated off-line against the traditional DTT assay using filter samples taken from urban environm

268 ith the latter transformation dependent upon DTT and an intact Fe-S center.

269 Global profiling of gene expression upon DTT treatment revealed a network of AP2 transcription fa

270 d if lysates were pretreated with 8 M urea + DTT.

271 ere treated with denaturants (SDS, 8 M urea, DTT, or trypsin) before BNP.

272 ccomplished in a reducing environment, using DTT as an external stimulus, and the thiol constituents

273 carried out at different temperatures using DTT(ox) as the oxidizing agent, and the results were com

274 original bio-barcode assay method, utilizing DTT, has streamlined and simplified probe preparation an

275 ung metastases further confirmed the variant DTT-IYG to be the best at restoring wild-type-like prope

276 d one [4Fe-4S](2+) cluster per homodimer via DTT-induced two-electron reductive coupling of two [2Fe-

277 hibited detectable cell-fusing activity when DTT was present.

278 r electron acceptor for ALR than oxygen when DTT is the reducing substrate.

279 With DTT, a new approximately 75 G wide radical EPR was obser

280 Treatment of 25 with DTT in neutral buffer at room temperature demonstrated t

281 0% of the activity with Trx as compared with DTT, raising the possibility that, in animal cells, Trx

282 substances, HULIS), was also correlated with DTT activity in both the water (R = 0.78) and methanol e

283 2S](2+) cluster by anaerobic incubation with DTT and Fe(2+) ion in the absence of exogenous sulfide.

284 e, we report a novel technique, Instant with DTT, EDT, And Low temperature (IDEAL)-labeling, for rapi

285 s A and C, TBE-31 may directly interact with DTT and protein targets such as Keap1 that contain react

286 es that the surfactants do not interact with DTT uniformly, instead concentrating in the most hydroph

287 nstitutive HAC1 splicing that interacts with DTT-mediated perturbation of protein folding.

288 the spectrum of tract lengths obtained with DTT closely matches that estimated from histological rec

289 tion appeared better in samples reduced with DTT in MeOH.

290 For in-gel rehydration, samples reduced with DTT were diluted with sample buffer containing 2-hydroxy

291 Reduction with DTT significantly potentiated GABA-induced currents in a

292 reductase activity that was reversible with DTT treatment, whereas graded cross-link lengthening gra

293 lished when the Wnt peptide was treated with DTT, and did not occur with a linear (non-disulfide-bond

294 al alpha-azido ester group, was treated with DTT/DIPEA.

295 enzyme can be reactivated by treatment with DTT and Fe(II).

296 ame antibody accessible after treatment with DTT, suggesting that the N termini are linked by interch

297 ral semiquinone during aerobic turnover with DTT.

298 Without DTT, EPR showed a mixture of superoxide and biopterin ra

299 The digests were treated with or without DTT and analyzed by SDS-PAGE and Western blotting.

300 t only when the digests were treated without DTT.