ライフサイエンスコーパス: E. faecium

1 E. faecium has been categorized as belonging to three cl

2 E. faecium is able to produce hydrogen peroxide by using

3 E. faecium isolates (n=577) from diverse sources were sc

4 E. faecium isolates showed high resistance to vancomycin

5 E. faecium was isolated from 105 patients, 65 vegetarian

6 eus, 70 S. lugdunensis, 121 E. faecalis, 100 E. faecium, 578 Enterobacterales, 142 Haemophilus spp.,

7 the United Kingdom and Ireland (UK&I) and 11 E. faecium isolates from a reference collection.

8 ly, antibodies to Acm were present in all 14 E. faecium endocarditis patient sera.

9 y of 181 Enterococcus faecalis isolates, 157 E. faecium isolates, and 60 isolates of other species; h

10 ophoresis (PFGE) revealed that all of the 19 E. faecium isolates with the VanB phenotype had identica

11 ded 108 (69%) E. faecalis isolates, 46 (29%) E. faecium isolates, and 1 isolate each of E. avium, E.

12 Despite the detection of acm in 32 out of 32 E. faecium isolates, only 11 of these (all clinical isol

13 . flavescens [n = 10], E. faecalis [n = 34], E. faecium [n = 43], E. avium [n = 1], E. gallinarum [n

14 f 3436 S. aureus, 1362 K. pneumoniae and 348 E. faecium samples, ROC curves demonstrate that the cons

15 aecium populations, we have now assessed 433 E. faecium isolates, including 264 isolates from human c

16 Among 481 E. faecium isolates belonging to the hospital-adapted cl

17 dertook whole-genome sequencing (WGS) of 495 E. faecium bloodstream isolates from 2001-2011 in the Un

18 Among 75 isolates, 50 E. faecium and 6 E. faecalis passed WGS quality threshol

19 rococcus faecalis isolates, 30 of 58 (51.7%) E. faecium isolates, 1 of 1 E. raffinosus isolate, 0 of

20 Here we analyzed 90 E. faecium isolates (99% acm(+)) and found that the Acm

21 ve determined the structure of the activated E. faecium LiaR protein at 3.2A resolution and, in combi

22 mental contamination by the hospital-adapted E. faecium lineage were hyperendemic in our study popula

23 lus three additional C. coli, one additional E. faecium, and one C. jejuni also developed resistance

24 ,4-triazolo[1,5-a]pyrimidines active against E. faecium is reported herein.

25 rrow-spectrum antibacterial activity against E. faecium and exhibited metabolic stability with low in

26 id while ridinilazole were effective against E. faecium only.

27 jor (VM) errors (4/14) with enterococci (all E. faecium) but none (0/22) with staphylococci.

28 ted as resistant by 100% of laboratories; an E. faecium strain with moderate-level resistance (MIC, 6

29 in, purified from culture supernatants of an E. faecium clinical isolate, was found to match the N-te

30 fied in Enterococcus faecium by screening an E. faecium genomic expression library with sera from pat

31 , and tetracycline resistance from C68 to an E. faecium recipient strain occurs at low frequency in v

32 r polysaccharides from E. faecalis 12030 and E. faecium 838970 were purified, and chemical and struct

33 ated drug resistant strains of S. aureus and E. faecium.

34 Antimicrobial-resistant E. coli and E. faecium have become significant pathogens in oncology

35 ) and 3.1 x 10(1) CFU/mL or g of E. coli and E. faecium, respectively) sprout and water samples teste

36 ones overall and centered around E. coli and E. faecium.

37 sent in both npmA2-carrying C. difficile and E. faecium clinical isolates.

38 own to effectively kill both E. faecalis and E. faecium (including vancomycin-resistant strains), as

39 icillin-imipenem tested with E. faecalis and E. faecium by BMD was >/=94% but was </=90% for other en

40 ntify enterococci other than E. faecalis and E. faecium can be compensated for by the addition of sta

41 ce in Enterococcus including E. faecalis and E. faecium highlights the significance of genomic survei

42 The E. faecalis and E. faecium isolates differed in their susceptibilities t

43 species, we found that both E. faecalis and E. faecium kill C. elegans eggs and hatchlings, although

44 conferred protection against E. faecalis and E. faecium strains in a mouse infection model.

45 isolates and (10(0) CFU/mL) E. faecalis and E. faecium strains were detected within 4 and 8 h of pre

46 s observed against different E. faecalis and E. faecium strains.

47 icillin-imipenem tested with E. faecalis and E. faecium was >/=98% and was 92% for other enterococci;

48 Enterococcus faecalis and E. faecium were isolated from diverse hosts highlighting

49 between vancomycin-resistant E. faecalis and E. faecium.

50 esis of livestock-associated E. faecalis and E. faecium.

51 ), ST6 (n = 3), and ST185 (n = 1), which are E. faecium sequence types belonging to clonal complex 5

52 ntage of enterococci that were identified as E. faecium increased from 12.7 to 22.2% (P < 0.001) and

53 been previously phenotypically identified as E. faecium.

54 why antibiotic-resistant hospital-associated E. faecium are often replaced by clade B strains once pa

55 solates, are part of the hospital-associated E. faecium genogroup referred to as clonal complex 17 (C

56 ion and dissemination of hospital-associated E. faecium in the UK&I and provide evidence for WGS as a

57 by alternate methods, they were found to be E. faecium.

58 ned with brain heart infusion broth for both E. faecium U8304 and E. faecalis V583.

59 potent efficacy, with CRS3123 reducing both E. faecium and E. faecalis burden similar to linezolid w

60 of identifying patients colonized with both E. faecium and E. faecalis, a feature useful for infecti

61 caused by E. faecalis and 37% were caused by E. faecium.

62 nts), at doses that promoted colonization by E. faecium C68.

63 hat promote gastrointestinal colonization by E. faecium have not been identified.

64 s suggest that hydrogen peroxide produced by E. faecium has cytotoxic effects and highlight the utili

65 analysis revealed that CVM1869 was a canine E. faecium clone that had acquired Tn1546, perhaps from

66 d E. faecalis, E. mundtii, E. casseliflavus, E. faecium, E. hirae, E. avium, and E. durans, respectiv

67 genes were frequently present in 30 clinical E. faecium isolates studied; one of these, acm, has been

68 ene was not identified in any of 34 clinical E. faecium isolates or in 4 other less pathogenic entero

69 high-level ampicillin resistance in clinical E. faecium strains.

70 butes to intestinal colonization in clinical E. faecium strains.

71 he molecular basis of resistance of clinical E. faecium PBP5 variants, results that are likely applic

72 bility of a colonization-proficient clinical E. faecium isolate (C68) to transfer colonizing ability

73 r previous studies showed that some clinical E. faecium isolates produce a cell wall-anchored collage

74 are no reports experimentally demonstrating E. faecium virulence determinants.

75 wo or more stool samples, 40 (40%) developed E. faecium carriage after admission based on culture, co

76 olonization levels by strains from different E. faecium lineages: clade B, part of the healthy human

77 al biochemical methods fail to differentiate E. faecium from certain newly described enterococcal spe

78 We suggest that actively dividing E. faecium cells have three zones of unique peptidoglyca

79 tive virulence gene associated with epidemic E. faecium strains.

80 In conclusion, the extracelluar E. faecium SagA protein is apparently essential for grow

81 s pore-forming toxins (Epxs) in E. faecalis, E. faecium, and E. hirae strains isolated across the glo

82 ococcus species, 633 strains of E. faecalis, E. faecium, and other enterococci isolated from blood cu

83 gative staphylococci, Enterococcus faecalis, E. faecium, E. avium, E. durans, E. casseliflavus, and E

84 including the species Enterococcus faecalis, E. faecium, E. casseliflavus, and E. gallinarum.

85 colonize humans, only Enterococcus faecalis, E. faecium, E. raffinosus, and E. casseliflavus have bee

86 We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its s

87 istin-dalfopristin resistance in human fecal E. faecium.

88 For penicillin, three of five E. faecium strains but none of five Enterococcus faecali

89 e resistance development in four out of five E. faecium strains; however, increased resistance was ob

90 For E. faecium and other enterococci, the combination of FS

91 For E. faecium, a susceptible dose-dependent (SDD) breakpoin

92 For E. faecium, categorical agreement was poor between the r

93 in-imipenem was 91% for E. faecalis, 98% for E. faecium, and 87% for other enterococci.

94 Genomes were also available for E. faecium associated with bloodstream infections in 15

95 22) were taken at intervals and cultured for E. faecium.

96 terococcus faecium (VREfm) is the driver for E. faecium carriage in hospitalized patients, which, in

97 ose for the reference method, especially for E. faecium isolates.

98 ggesting that the sagA gene is essential for E. faecium growth and may be involved in cell wall metab

99 SagA is, therefore, essential for E. faecium growth, stress resistance, and activation of

100 m the first factor shown to be important for E. faecium pathogenesis.

101 owever, all the Emp pilins are important for E. faecium to cause infection in the urinary tract.

102 n factors, was required in the intestine for E. faecium-mediated protection of C. elegans against a l

103 re 63%, 63%, 63%, and 56%, respectively, for E. faecium, 87%, 83%, 98%, and 80%, respectively, for E.

104 Rectal or fecal samples for E. faecium testing were obtained from 567 newly admitted

105 from 94 of these isolates into plasmid-free E. faecium GE-1 at transfer frequencies of <10(-9) to 10

106 y affinity-purified anti-Acm antibodies from E. faecium endocarditis patient sera, suggesting that Ac

107 ied four distinct surface carbohydrates from E. faecium endocarditis isolate Tx16, shown previously t

108 quence of domain V of the 23S rRNA gene from E. faecium and E. faecalis differed from those of all ot

109 The structure of VanX from E. faecium has been solved by X-ray crystallography and

110 Co-culture with Epx2-harboring E. faecium damages human peripheral blood mononuclear ce

111 -dalfopristin resistance was absent in human E. faecium, but 56% of conventional poultry isolates wer

112 We identified E. faecium transposon insertion mutants with altered C.

113 re, we report that deletion of sagA impaired E. faecium growth and resulted in bulged and clustered e

114 ritavancin have different modes of action in E. faecium.

115 Deletion of ahyD in E. faecium resulted in increased formation of Ala-PG and

116 tin-dalfopristin resistance were assessed in E. faecium isolates, and resistance genes were identifie

117 This gene is highly enriched in E. faecium clinical isolates and absent in commensal iso

118 stem peptide (L-Lys substituted by D-iAsn in E. faecium) is both necessary and sufficient for peptide

119 and their involvement has been implicated in E. faecium as well.

120 PTS permease BepA is directly implicated in E. faecium pathogenesis.

121 2 excises to form a circular intermediate in E. faecium.

122 icin resistance and 1 for nitrofurantoin, in E. faecium isolates.

123 bition of transpeptidation by oritavancin in E. faecium is the result of the large number of secondar

124 When overexpressed in E. faecium and Escherichia coli, the native and recombin

125 ), encoded immediately adjacent to rakPGS in E. faecium, which is responsible for the hydrolysis of a

126 , we demonstrated a significant reduction in E. faecium collagen adherence by affinity-purified anti-

127 However, the functions of SagA in E. faecium were unknown.

128 tial impact on colonization and virulence in E. faecium and possibly other Gram-positive bacterial sp

129 iants are unable to create a PG cell wall in E. faecium.

130 genesis of Gram-positive bacteria, including E. faecium We previously demonstrated that a nonpiliated

131 tomycin MICs of 3-4 microg/mL in the initial E. faecium blood isolate predicted microbiological failu

132 Six patients (3.4%) developed an invasive E. faecium infection from their own gut-colonizing strai

133 Kingdom was obtained and cultured to isolate E. faecium, ampicillin-resistant E. faecium (AREfm), and

134 A comparison of the E. faecalis LiaR, E. faecium LiaR, and the LiaR homolog from Staphylococcu

135 madagascariensis (S. aureus: MIC: 32 mug/mL; E. faecium: MIC: 32 mug/mL) stem bark.

136 in the clinical importance of multiresistant E. faecium across different continents.

137 d highly related vanA-positive and -negative E. faecium, which implies that control of vancomycin-res

138 transfer colonizing ability to noncolonizing E. faecium recipient strains.

139 anced surveillance and control of nosocomial E. faecium transmission and infection.

140 Correspondingly, E. faecalis, but not E. faecium, degrades the glycan on RNase B during growth

141 . lugdunensis, 98.3% of E. faecalis, 100% of E. faecium, and 99.6% of Enterobacterales.

142 vatE was present in 38% of E. faecium isolates from patients and none from vegetari

143 pristin resistance was found in 51 to 78% of E. faecium isolates from the food production environment

144 The vanA gene was detected in 93.9% of E. faecium isolates; other resistance determinants inclu

145 imary adhesin responsible for the ability of E. faecium to bind collagen.

146 s PTS, significantly impaired the ability of E. faecium to colonize the murine intestinal tract durin

147 on/acquisition has favored the adaptation of E. faecium to nosocomial environments and/or patients.

148 emonstrate that Fnm affects the adherence of E. faecium to fibronectin and is important in the pathog

149 -fold decrease (P < 0.05) in the adhesion of E. faecium TX82 to fibronectin.

150 ated epidemiological and genomic analysis of E. faecium associated with bloodstream infection and iso

151 ould significantly enhance the attachment of E. faecium cells onto the surface of silica sands and th

152 The disparity in the transport behavior of E. faecium with and without esp could limit the effectiv

153 nfluence of esp on the transport behavior of E. faecium.

154 ctional acm gene is necessary for binding of E. faecium strains to CI.

155 native Acm is sufficient for the binding of E. faecium to CI.

156 nd this gene is located on the chromosome of E. faecium 664.1H1, on a novel conjugative transposon.

157 Integration of Tn5382 in the chromosome of E. faecium C68 has occurred 113 bp downstream of the sto

158 SBP prophylaxis enriches complexity of E. faecium-centered but induces a collapse in E. coli-ce

159 pe could be rescued by a chromosomal copy of E. faecium GroEL/GroES or by expressing a recalcitrant R

160 c gene is associated with reduced density of E. faecium.

161 a nonpiliated DeltaempABC::cat derivative of E. faecium TX82 was attenuated in biofilm formation and

162 that Acm has contributed to the emergence of E. faecium and CC17 in nosocomial infections.

163 The frequency of E. faecium occurrence increased from 6% in the dry perio

164 ptococcus pneumoniae pavA, in the genomes of E. faecium strain TX82 and all other sequenced E. faeciu

165 We also show that CcpA affects the growth of E. faecium, that an intact ccpA gene is important for fu

166 implicating a role for CcpA in the growth of E. faecium.

167 as developed for the rapid identification of E. faecium.

168 terococcus faecalis inhibits the invasion of E. faecium invasion.

169 ltransferase from a human urinary isolate of E. faecium, has been determined as an apoenzyme and in c

170 three collagen-binding clinical isolates of E. faecium tested, but in none of the strains with a non

171 in all other tests, while the 53 isolates of E. faecium were ARA positive only.

172 rtain infection-derived clinical isolates of E. faecium, and suggest that Acm is the primary adhesin

173 apeutic alternatives because the majority of E. faecium isolates are resistant to ampicillin and vanc

174 colonization and dissemination mechanisms of E. faecium and vancomycin-resistant E. faecium.

175 silica sands and thus lower the mobility of E. faecium within sand packs.

176 or country, indicating frequent movement of E. faecium between regions that rarely share hospital pa

177 were isolated during a hospital outbreak of E. faecium with plasmid-mediated VanB resistance.

178 G and stem peptides are recruited to PBP5 of E. faecium, a critical ESKAPE pathogen.

179 Twenty-two percent of E. faecium isolates showed reduced susceptibility to qui

180 Both the percentage of E. faecium among the enterococci and the proportion of v

181 993 to 2002, we evaluated the percentages of E. faecium among all enterococcal isolates and the perce

182 enterococcal isolates and the percentages of E. faecium isolates that were vancomycin resistant.

183 study, we provide a comprehensive picture of E. faecium transmission in an entire sampled patient pop

184 s reveal a diverse, polyclonal population of E. faecium harboring high-risk resistance and virulence

185 e gastrointestinal (carriage) populations of E. faecium.

186 c free) were also tested for the presence of E. faecium.

187 7 to 22.2% (P < 0.001) and the proportion of E. faecium that was vancomycin resistant increased from

188 c support for the tetracycline resistance of E. faecium 664.1H1 was characterized.

189 ycin-exposed group, daptomycin resistance of E. faecium from the off-target population was on average

190 study in which the daptomycin resistance of E. faecium isolated from rectal swabs from daptomycin-ex

191 plain the effects of esp on the retention of E. faecium.

192 issemination of a highly resistant strain of E. faecium among patients and among other extant VRE str

193 e clonal dissemination of a single strain of E. faecium VanB.

194 Notably, diverse strains of E. faecium produce SagA, a highly conserved peptidoglyca

195 Interestingly, a high titer of E. faecium also accumulates in the nematode gut, but doe

196 glycopeptide resistance element, Tn1546, of E. faecium 228 was used as the basis of comparison for a

197 which will inform the future translation of E. faecium sequencing into routine outbreak detection an

198 cannot be eradicated by prolonged feeding on E. faecium.

199 One E. faecium isolate, CVM1869, displayed high-level resist

200 urrent study, one E. faecalis strain and one E. faecium strain carried only the vanC gene.

201 ts for 45 of 46 cultures with E. faecalis or E. faecium.

202 er of VR isolates, belonging to esp-positive E. faecium, was revealed.

203 nents of a glycoconjugate vaccine to prevent E. faecium infection.

204 enic strain overproducing catalase prevented E. faecium-mediated killing.

205 on correlated with mutations in the putative E. faecium capsular polysaccharide (cps) biosynthetic lo

206 e, ceftriaxone, or amoxicillin against DAP-R E. faecium R497 using established in vitro and in vivo m

207 amoxicillin was efficacious against a DAP-R E. faecium strain, and pbp5 alleles may be important con

208 e combinations against DAP-resistant (DAP-R) E. faecium strains is unknown.

209 ) purified against these subsegments reduced E. faecium TX2535 strain collagen adherence up to 73 and

210 early US AMP isolates were clonally related, E. faecium CC17 isolates have been circulating in the Un

211 to isolate E. faecium, ampicillin-resistant E. faecium (AREfm), and VREfm.

212 , Lc-Lys could lyse the ampicillin-resistant E. faecium mutant with 3-->3 L-Lys(3)-D-Asn-L-Lys(3) bri

213 Quinupristin-dalfopristin-resistant E. faecium contaminates a large proportion of chickens s

214 Quinupristin-dalfopristin-resistant E. faecium was isolated from 237 chicken carcasses and 3

215 uency of quinupristin-dalfopristin-resistant E. faecium, we used selective medium to culture samples

216 riage of quinupristin-dalfopristin-resistant E. faecium.

217 d the fecal shedding of daptomycin-resistant E. faecium by up to 80-fold.

218 mpetent mouse model of acute, drug-resistant E. faecium infection.

219 therapeutic strategy to treat drug-resistant E. faecium infection.

220 ear both E. faecalis and multidrug-resistant E. faecium.

221 se the potential for streptogramin-resistant E. faecium infection in humans.

222 al [CI], 6.20-8.76) and vancomycin-resistant E. faecium (rate ratio, 2.27, 95% CI, 2.03-2.53).

223 utic approaches against vancomycin-resistant E. faecium and also reveal the intricate interrelationsh

224 ypic characteristics of vancomycin-resistant E. faecium and E. faecalis clinical isolates from a tert

225 erms of the recovery of vancomycin-resistant E. faecium and E. faecalis, the sensitivity and PPV were

226 fferentiating IVRE from vancomycin-resistant E. faecium and E. faecalis; however, a combination of LM

227 m from three CI-binding vancomycin-resistant E. faecium clinical isolates showed 100% identity, analy

228 ee clinical isolates of vancomycin-resistant E. faecium collected from 3 patients during a 6-week per

229 rog/mL, show almost all vancomycin-resistant E. faecium have AUICs <125.

230 alfopristin, a drug for vancomycin-resistant E. faecium in humans.

231 i and the proportion of vancomycin-resistant E. faecium increased significantly over this 10-year per

232 One vancomycin-resistant E. faecium isolate was highly resistant to both teicopla

233 s the first report of a vancomycin-resistant E. faecium isolated from a companion animal in the Unite

234 in this study were all vancomycin-resistant E. faecium isolates collected from 28 hospitals and thre

235 were found primarily in vancomycin-resistant E. faecium isolates in nonstool cultures obtained from p

236 Nineteen (90%) of the vancomycin-resistant E. faecium isolates were of the VanB phenotype, with van

237 aecalis strain and on a vancomycin-resistant E. faecium strain.

238 spital dissemination of vancomycin-resistant E. faecium strains over a 6-year period in southeastern

239 faecalis strains and 7 vancomycin-resistant E. faecium strains possess shared capsular polysaccharid

240 calis and 4 isolates of vancomycin-resistant E. faecium that were not recovered by BEAV.

241 ted to the emergence of vancomycin-resistant E. faecium, which causes many hospital-acquired infectio

242 6, perhaps from a human vancomycin-resistant E. faecium.

243 to treat culture-proven vancomycin-resistant E. faecium.

244 nisms of E. faecium and vancomycin-resistant E. faecium.

245 hybrids of the RseP proteins from sensitive E. faecium and insensitive L. plantarum, we showed that

246 ancomycin-resistant and vancomycin-sensitive E. faecium, E. faecalis, E. hirae, and E. durans.

247 faecium strain TX82 and all other sequenced E. faecium isolates.

248 Here we show E. faecium has unique peptidoglycan composition and remo

249 We previously showed that some E. faecium strains express a cell wall-anchored collagen

250 e 16S rRNA sequences of enterococcal species E. faecium, E. faecalis, E. gallinarum, E. casseliflavus

251 These data suggest that specific E. faecium strains may be enriched in determinants that

252 d accurate detection of Staphylococcus spp., E. faecium, and E. faecalis and its ability to ascertain

253 nsferred DNA into a second recipient strain (E. faecium GE-1), which also colonized mice in significa

254 d be used cautiously against DAP-susceptible E. faecium strains with minimum inhibitory concentration

255 ires consideration of vancomycin-susceptible E. faecium Our findings reveal the evolution and dissemi

256 th vanA and vanB], 93 vancomycin-susceptible E. faecium) and epidemiological data were collected.

257 er mobility within saturated sand packs than E. faecium strains without esp.

258 g and phenotypic analysis, we determine that E. faecium isolates are pan-resistant to aminoglycosides

259 an indicator of toxicity, we determined that E. faecium produces hydrogen peroxide at levels that cau

260 These results indicate that E. faecium clinical isolates express transmissible facto

261 dex versus persistent isolates revealed that E. faecium may be genetically preadapted to cause persis

262 Finally, we show that E. faecium and E. faecalis occupy overlapping but distin

263 We previously showed that E. faecium strains with daptomycin minimum inhibitory co

264 Overall, our results suggested that E. faecium strains with esp could display lower mobility

265 The E. faecium sagA gene, like the S. mutans homologue, is l

266 ber of colony-forming units per gram) by the E. faecium clades at any time point (P > .05).

267 of a secondary binding interaction with the E. faecium peptidoglycan.

268 Therefore, E. faecium-LR9 is a promising strain for the release of

269 were detected in seven E. faecalis and three E. faecium isolates.

270 ed d-Ala-d-Ala binding pocket still binds to E. faecium peptidoglycan.

271 AR-1 pathway were activated upon exposure to E. faecium in an HLH-26-dependent manner.

272 ng 53 clinical and geographically diverse US E. faecium isolates dating from 1971 to 1994, we determi

273 ansconjugants derived from matings that used E. faecium D344SRF as a recipient strain colonized mouse

274 Using E. faecium-mediated killing of the nematode worm Caenorh

275 on of an altered Tn1546 in heterologous VanA E. faecium in Michigan hospitals.

276 s study shows that in a majority of the VanA E. faecium isolates, Tn1546 is altered compared to that

277 Variations among vanA and vanB E. faecium isolates from individual long-term-colonized

278 evalence of the other 14 genes among various E. faecium populations, we have now assessed 433 E. faec

279 VR E. faecium isolates were less genetically diverse than v

280 f VR E. faecalis isolates and none of the VR E. faecium isolates.

281 istration approval for the treatment of VRE (E. faecium) infections, namely, linezolid and quinuprist

282 terococci are Enterococcus faecalis, whereas E. faecium accounts for 5 to 10% of isolates.

283 to collagen, and this protein competed with E. faecium binding to immobilized CI.

284 h antibiotic resistance were correlated with E. faecium.

285 United Kingdom identified 342 patients with E. faecium bloodstream infection over 7 years.

286 hort study that included adult patients with E. faecium BSI for whom initial isolates, follow-up bloo

287 We postulate that patients with E. faecium BSIs exhibiting daptomycin MICs of 3-4 microg

288 Thirty-seven of 41 sera from patients with E. faecium infections showed reactivity with recombinant

289 ression library with sera from patients with E. faecium-associated endocarditis.

290 ophylaxis, whereas the reverse was seen with E. faecium.

291 lazole exhibited a broader MIC spectrum with E. faecium highly sensitive (< 0.007-0.25 ug/mL) and E.