ライフサイエンスコーパス: E2F6

1 st in part dependent on the association with E2F6.

2 reated Ntera2 cell lines harboring shRNAs to E2F6.

3 components and with the transcription factor E2F6.

4 all inhibitory ribonucleic acids specific to E2F6.

5 mily members, one promoter was bound only by E2F6.

6 inds within the known "repression domain" of E2F6.

7 YC, and regulated by ZNF274, MAX, IKZF3, and E2F6.

8 we demonstrate that the transcription factor E2F6, a member of the polycomb repressive complex 1.6 (P

9 E2F6, a repressor of E2F-dependent G1/S transcription, r

10 Indeed, inhibition of both E2F4 and E2F6 activity results in specific derepression of these

11 PRC1L4 also contained E2F6 and CBX3/HP1gamma, known to function in transcripti

12 ot mutant E2F promoters by repressive PRC1.6/E2F6 and DREAM/E2F4 complexes.

13 ant E2Fs, whereas AtE2F2 is related to human E2F6 and Drosophila dE2F2 which are unusual in lacking t

14 demonstrate that BRG1 binds specifically to E2F6 and E2F4 but not the activator E2Fs.

15 The 5' untranslated regions of E2F6 and E2F6b are unusually long, and they contain seve

16 We also show that E2F6 and E2F6b mRNAs are ubiquitously expressed in prima

17 the E2f6 locus produces two different mRNAs, E2F6 and E2F6b.

18 ave identified 48 endogenous target genes of E2F6 and have shown that E2F6 can repress target promote

19 ts, including the transcriptional repressors E2f6 and Mga, in mitotic spermatogonia.

20 Moreover, we demonstrate that endogenous E2F6 and polycomb group proteins, including RYBP, Ring1,

21 and E2F3a) or repressors (E2F3b, E2F4, E2F5, E2F6, and E2F7).

22 ell cycle transcription factors (E2F1, E2F4, E2F6, and GABPA) from the Encyclopedia of DNA Elements a

23 We further show that MGA-MAX, E2F6, and L3MBTL2 co-occupy thousands of promoters and t

24 MEIOC thereby derepresses E2F6- and MGA-repressed genes, including Meiosin and oth

25 gs suggest that the biological properties of E2F6 are mediated through its ability to recruit the pol

26 atabase identified transcriptional repressor E2F6 as a possible negative regulator of MDM2 expression

27 on assays to assess when and with what genes E2F6 associates during a cell cycle.

28 We find that E2F6 associates specifically with the E2F target genes t

29 We determined that most E2F1, E2F4, and E2F6 binding sites are located within 2 kb of a transcri

30 , selectively driving its recruitment toward E2F6-binding motifs.

31 Genome-wide, E2F6 binds preferentially to CpG islands in embryonic ce

32 that a large set of promoters were bound by E2F6, but not by E2F1 or E2F4.

33 e regulated by E2F6, we reduced the level of E2F6 by using RNA interference technology.

34 Consistent with this finding, E2F6 can behave as a dominant negative inhibitor of the

35 ous target genes of E2F6 and have shown that E2F6 can repress target promoters in a manner that does

36 However, E2F6 cannot bind to all promoters that contain consensus

37 complex, resembling the previously described E2F6-complex, and including G9A, Hdac1, and Ring1b.

38 that HPV16 E7 associates with and diminishes E2F6-containing polycomb repressive complexes.

39 E2F6 contains a DNA binding domain that is very similar

40 It has been proposed that E2F6 contributes to gene silencing by recruiting enzymes

41 E2F6 cooperates with MGA to silence a subgroup of germli

42 ow that E2F-dependent transcription, through E2F6, determines the replication capacity of a cell, def

43 In sharp contrast, E2F6 does not bind to E2F-regulated genes activated at G

44 latory profiles of several cellular factors (E2F6, E2F1, Rb, HDAC1, and HDAC2) together with EBV late

45 In the absence of E2F6, E2F4 can bind to the G1/S-regulated promoters and

46 regulation of cell cycle-related genes, with E2F6, E2F7, and E2F8 playing key roles in repression.

47 e for altering E2F1-E2F5 expression, but not E2F6-E2F8 expression.

48 E2F6 expression is activated in S phase through an E2F-d

49 an effort to better delineate the context of E2F6 function, including the mechanisms of E2F6 function

50 gulated promoters and compensate for loss of E2F6 function.

51 f E2F6 function, including the mechanisms of E2F6 functional specificity, we used chromatin immunopre

52 We conclude that E2F6 functions as a repressor of E2F-dependent transcrip

53 or the G1/S-regulated genes, we propose that E2F6 functions to distinguish G1/S and G2/M transcriptio

54 E2F6 has been postulated to mediate transcriptional repr

55 c microarrays to identify promoters bound by E2F6 in human cells.

56 usly provided evidence suggesting a role for E2F6 in repression of E2F-responsive genes at S phase.

57 tinue to investigate the possible role(s) of E2F6 in vivo.

58 SWI/SNF chromatin-remodeling complex, as an E2F6 interacting protein.

59 ber of the mammalian polycomb complex, as an E2F6-interacting protein.

60 E2F6 is a component of polycomb group complexes, which b

61 E2F6 is a pRB-independent, noncanonical member of the E2

62 edundancy in the E2F family and suggest that E2F6 is not critical for histone methylation.

63 Furthermore, E2F6 is required to initiate epigenetic silencing in ear

64 E2F6 is the most recently identified and the least well

65 translation initiation can produce distinct E2F6 isoforms under different physiological conditions.

66 s, the checkpoint kinase Chk1 phosphorylates E2F6, leading to its dissociation from promoters.

67 Inactivation of E2f6 leads to a failure to deposit CpG island DNA methyl

68 It has recently been shown that the E2f6 locus produces two different mRNAs, E2F6 and E2F6b.

69 s, a function that critically depends on the E2F6 marked box domain.

70 Collectively, our studies suggest that E2F6 may recruit BRG1 in transcriptional regulation of g

71 on the top candidates suggests that REST and E2F6 may serve as key regulators in the NEPC progression

72 ptional activity of E2F family members (E2F1-E2F6), most of them regulated by suppressive association

73 onstrate the binding of BRG1 coincident with E2F6 on G1/S gene promoters during S phase.

74 We found that reduction of E2F6 only induced minimal alteration of the transcriptom

75 sponse to cellular DNA damage, E2F7, but not E2F6 or E2F8, is up-regulated in a p53-dependent manner,

76 cells suggesting that PHC3 may be part of an E2F6-polycomb complex that has been shown to occupy and

77 expressing cells show decreased staining for E2F6/polycomb complexes and that this is at least in par

78 ed genes simultaneously bound by L3MBTL2 and E2F6, preferentially around transcriptional start sites

79 The E2F6 protein functions as an Rb-independent repressor of

80 s increased after reduction of the amount of E2F6 protein in the cell.

81 E2F proteins, the latter group including the E2F6 protein, which has been shown to function as an Rb-

82 s rise to E2F6b, an amino-terminal truncated E2F6 protein.

83 Interestingly, many of the E2F6-regulated genes encoded functions involved in tumor

84 However, we found that the E2F6-regulated promoters did not contain histone H3 meth

85 To determine the mechanism by which E2F6 regulates transcription, we performed chromatin imm

86 Specifically, our results suggest that E2F6 represses transcription of the brca1, ctip, art27,

87 We found that depletion of E2F6 resulted in the recruitment of E2F1 to the target p

88 ifferentiating and confluent cells, PHC3 and E2F6 showed nuclear colocalization in a punctate pattern

89 Our data show that E2F6 suppresses Mdm2 expression in cells harboring the S

90 E2F6, the most recently identified member of the E2F fam

91 e the transcriptional repression activity of E2F6, thereby subverting a critical cellular defense mec

92 E2F6 was also able to interact with BAF155, a BRG1-assoc

93 hat mRNA transcribed from promoters bound by E2F6 was increased after reduction of the amount of E2F6

94 newly identified promoters were regulated by E2F6, we reduced the level of E2F6 by using RNA interfer

95 opically expressed E2F1, -2, and -3, but not E2F6, were reduced after synthesis of p19ARF, through a