ライフサイエンスコーパス: ETD

1 ETD fragmentation produced diagnostic fragment ions indi

2 ETD improved phosphopeptide identification, while PQD pr

3 ETD-MS/MS of these labeled peptides also produces a peak

4 compared with liraglutide (-3.1 kg [SE 0.2]; ETD -1.2 kg, 95% CI -1.9 to -0.6; p=0.0003) and placebo

5 z (*) -type product ions resulting from 226 ETD product ion spectra can be assigned to a single, cor

6 formation of unique w-ions employing MS(3) (ETD-HCD) for rapid Ile/Leu distinction.

7 ght -3.7 kg (SE 0.3) versus -1.1 kg (SE 0.3; ETD -2.7 kg, 95% CI -3.5 to -1.9; p<0.0001).

8 imand: -2.9 kg [SE 0.3] vs -0.8 kg [SE 0.3], ETD -2.2 kg, -2.9 to -1.5; p<0.0001).

9 .6; p=0.0003) and placebo (-0.5 kg [SE 0.3]; ETD -3.8 kg, -4.7 to -3.0; p<0.0001) at week 26 (treatme

10 nge of -3.4 kg [SE 0.3] vs -0.9 kg [SE 0.3]; ETD, -2.5, 95% CI -3.2 to -1.8; p<0.0001) by the treatme

11 he ETD model was trained with more than 7000 ETD spectra, with and without supplemental activation.

12 entage points (SE 0.1; -1 mmol/mol [SE 0.8]; ETD -1.0 percentage points, 95% CI -1.2 to -0.8; p<0.000

13 rithm to score potential assignments against ETD-MS/MS data, we applied the method to glycopeptides g

14 AI-ETD achieves these gains through improved quality of fra

15 AI-ETD also outperformed HCD, generating more matching frag

16 AI-ETD increased the number of c- and z-type product ions f

17 AI-ETD provided the greatest sequence coverage for all five

18 AI-ETD, which leverages infrared photon bombardment concurr

19 andard ETD with a short reaction time and AI-ETD with a long reaction time.

20 illustrate the benefits of fiber-assisted AI-ETD, namely, a simplified system for irradiating the two

21 introduce a new activation scheme called AI-ETD+ that combines AI-ETD in the high pressure cell of t

22 ation scheme called AI-ETD+ that combines AI-ETD in the high pressure cell of the QLT with a short in

23 We conclude AI-ETD has the potential to rapidly and comprehensively ana

24 vated ion-electron transfer dissociation (AI-ETD) and IR multiphoton dissociation (IRMPD) experiments

25 vated ion electron transfer dissociation (AI-ETD) for top down protein characterization, showing that

26 vated ion electron transfer dissociation (AI-ETD) on a quadrupole-Orbitrap-linear ion trap hybrid MS

27 vated ion electron transfer dissociation (AI-ETD) uses concurrent infrared photoactivation to promote

28 vated ion electron transfer dissociation (AI-ETD), a recently introduced method for enhanced ETD frag

29 vated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,6

30 vated ion electron transfer dissociation (AI-ETD).

31 (ETD) reactions, i.e., activated ion ETD (AI-ETD), significantly increases dissociation efficiency re

32 When combined with HCD, AI-ETD improved the protein sequence coverage by more than

33 Most importantly, AI-ETD reveals disulfide-bound regions that have been intra

34 can significantly improve localization in AI-ETD spectra.

35 extensive generation of fragment ions in AI-ETD+ substantially increases peptide sequence coverage w

36 to generating more unique fragment ions, AI-ETD provided greater protein sequence coverage compared

37 In all, the effectiveness of AI-ETD across the entirety of the m/z spectrum demonstrates

38 on platform, permitting implementation of AI-ETD and IRMPD on commercial mass spectrometers and broad

39 ns due to the infrared photoactivation of AI-ETD and show that modifying phosphoRS (a phosphosite loc

40 Here we present the performance of AI-ETD for identifying and localizing sites of phosphorylat

41 Finally, we demonstrate the utility of AI-ETD in localizing phosphosites in alpha-casein, an appro

42 e we describe the first implementation of AI-ETD on a quadrupole-Orbitrap-quadrupole linear ion trap

43 Here, we present the first application of AI-ETD to mAb sequencing.

44 We then characterize the performance of AI-ETD using standard peptides in addition to a complex mix

45 First, we show that AI-ETD can be implemented in a straightforward manner by fa

46 Using 90 min analyses we show that AI-ETD can identify 24,503 localized phosphopeptide spectra

47 des using LC-MS/MS, showing not only that AI-ETD can nearly double the identifications achieved with

48 wn protein characterization, showing that AI-ETD definitively extends the m/z range over which ETD ca

49 this work highlights the analytical value AI-ETD can bring to both bottom-up and top-down phosphoprot

50 our analysis revealed that nearly 60% of all ETD-identified peptides carried two positive charges, wh

51 quantify peptide oxidation isomers using an ETD MS/MS spectrum acquired at any point across the sing

52 dicate that the search engines for analyzing ETD derived MS/MS spectra are still in their early days

53 verage over CID; and (iii) combining CID and ETD fragmentation increased the sequence coverage for an

54 nked peptide ions were identified by CID and ETD fragmentation, and the disulfide-dissociated (or par

55 characteristics were analyzed using CID and ETD tandem mass spectrometry.

56 d or electron transfer dissociation (CID and ETD).

57 by collision induced dissociation (CID) and ETD with linear quadrupole ion trap (LTQ)-Fourier transf

58 al CID (collision-induced dissociation)- and ETD (electron transfer dissociation)-MS/MS experiments.

59 of labile N-phosphorylated peptides, ECD and ETD are emerging as a complementary method.

60 results have for previously proposed ECD and ETD mechanisms are discussed.

61 n sequence coverage compared to both HCD and ETD.

62 cribed approach for online gas-phase HDX and ETD paves the way for making mass spectrometry technique

63 mass spectrometric analysis (HCD-MS(n)) and ETD (electron transfer dissociation)-HCD MS(3) analysis

64 Using the FLI tag and ETD, we identified Glu73 as the site of photoincorporati

65 Combining UVPD and ETD data afforded even deeper sequencing and greater ove

66 immonium ion as a diagnostic tool as well as ETD-based fragmentation techniques to achieve unambiguou

67 than either the Ascore (CAD) or the Ascore (ETD).

68 However, because ETD only yields comprehensive sequence coverage when the

69 teins can be proteolytically digested before ETD analysis, although digestion is not necessary for al

70 CI -7.10 to -3.57; p<0.0001; and bodyweight ETD -1.06 kg, 95% CI -1.76 to -0.36; p=0.0029).

71 tion of fluoranthene are often used for both ETD and PTR reactions; the radical anion of fluoranthene

72 complementary information derived from both ETD and CID dissociation methods, peptide sequence and p

73 egrating the product ion information of both ETD and CAD data are evident by increased confidence in

74 on method was facilitated by performing both ETD and UVPD within the higher energy collisional dissoc

75 sphorylated peptides can be characterized by ETD.

76 in ND(3) gas and subsequently fragmented by ETD.

77 the peptide backbone cannot be identified by ETD/HCD.

78 , the present work proposes (i) to reduce by ETD one of the two disulfide bridges of model peptides,

79 nating collision-induced dissociation (CID), ETD, and higher-energy collisional dissociation (HCD) sc

80 pes of spectra (including CID, HCD, ETD, CID/ETD and HCD/ETD spectra of trypsin, LysC or AspN digeste

81 spectra or even for spectral pairs (e.g. CID/ETD spectral pairs).

82 The novel HCD-pd-CID/ETD workflow combines the best possible decision tree de

83 y high and the m/z ratio is low, we combined ETD with a targeted chemical derivatization strategy to

84 f 10 mg or more vs placebo (dosage-dependent ETD range, -0.9 to -5.7 kg; P < .001).

85 ing HbA(1c) (estimated treatment difference [ETD] -0.1%, 95% CI -0.3 to 0.0; p<0.0001) and superior t

86 zin (HbA(1c) estimated treatment difference [ETD] -0.49 percentage points, 95% CI -0.65 to -0.33; -5.

87 kg [SE 0.2], estimated treatment difference [ETD] -1.9 kg, 95% CI -2.6 to -1.2; p<0.0001; and trial p

88 ge-dependent estimated treatment difference [ETD] range for oral semaglutide vs placebo, -0.4% to -1.

89 rgine group; estimated treatment difference [ETD], -0.59% [95% CI, -0.74% to -0.45%]), meeting criter

90 l [SE 0.8]); estimated treatment difference [ETD]: -0.8 percentage points, 95% CI -1.0 to -0.6; p<0.0

91 C-MS/MS with electron-transfer dissociation (ETD) alone is used for the structural characterization o

92 Electron-transfer dissociation (ETD) analysis indicates the presence of K48 in these bra

93 mbination of electron-transfer dissociation (ETD) and collision induced dissociation (CID) fragmentat

94 onsisting of electron transfer dissociation (ETD) and collision induced dissociation (CID), in combin

95 g sequential electron transfer dissociation (ETD) and collision-induced dissociation (CID) steps, in

96 rediction of electron-transfer dissociation (ETD) and electron-capture dissociation (ECD) spectra of

97 ely obtained electron transfer dissociation (ETD) and higher-energy collisional dissociation (HCD) ta

98 fitted with electron transfer dissociation (ETD) between the quadrupole and mobility regions prior t

99 ry (MS) with electron transfer dissociation (ETD) capabilities.

100 od involving electron transfer dissociation (ETD) combined with ultraviolet photodissociation (UVPD)

101 occur under electron transfer dissociation (ETD) conditions, including nondissociative electron tran

102 Electron-transfer dissociation (ETD) constitutes a valuable tool to cleave the disulfide

103 The use of electron transfer dissociation (ETD) facilitates this analysis because disulfide bonds a

104 ype protein, electron transfer dissociation (ETD) fragmentation has been used to pinpoint the residue

105 n (HCD), and electron transfer dissociation (ETD) fragmentation modes.

106 d species in electron-transfer dissociation (ETD) fragmentation.

107 Electron transfer dissociation (ETD) has improved the mass spectrometric analysis of pro

108 Electron transfer dissociation (ETD) has proven to be a promising new ion activation met

109 more common electron transfer dissociation (ETD) have been introduced and made widely available.

110 ion (ECD) or electron-transfer dissociation (ETD) have been successfully used for comprehensive phosp

111 outperformed electron transfer dissociation (ETD) in terms of sequence coverage, allowing the SETA re

112 n (HCD), and electron transfer dissociation (ETD) in terms of yielding the most comprehensive diagnos

113 on (ECD) and electron transfer dissociation (ETD) involve radical-driven fragmentation of charge-redu

114 Electron transfer dissociation (ETD) is a recently introduced mass spectrometric techniq

115 Electron transfer dissociation (ETD) is commonly used in fragmenting N-linked glycopepti

116 Electron transfer dissociation (ETD) is increasingly becoming popular for high-throughpu

117 Electron transfer dissociation (ETD) is the method of choice in analyzing these glycopep

118 pairs using electron transfer dissociation (ETD) markedly reduced adduct loss.

119 bitrap Velos electron-transfer dissociation (ETD) mass spectrometer (MS) was established to simultane

120 confirmed by electron transfer dissociation (ETD) mass spectrometry.

121 aphy (LC) to electron-transfer dissociation (ETD) mass spectrometry.

122 entional ECD/electron transfer dissociation (ETD) methods because it can be implemented using a stand

123 n (HCD), and electron transfer dissociation (ETD) MS/MS approach obtained 80% protein sequence covera

124 workflow, an electron-transfer dissociation (ETD) MS2 was performed and analyzed in the ion trap.

125 strated that electron transfer dissociation (ETD) of 4-plex iTRAQ labeled peptides cleaves at the N-C

126 on (ECD) and electron transfer dissociation (ETD) of doubly protonated electron affinity (EA)-tuned p

127 on (CAD) and electron transfer dissociation (ETD) on a single instrument.

128 on (CID) and electron-transfer dissociation (ETD) on each precursor on a liquid chromatography-mass s

129 XL with the electron transfer dissociation (ETD) option.

130 tal-assisted electron transfer dissociation (ETD) or electron capture dissociation (ECD) provide vary

131 on (CID) and electron transfer dissociation (ETD) processes.

132 ation during electron transfer dissociation (ETD) reactions, i.e., activated ion ETD (AI-ETD), signif

133 nd 2981 from electron transfer dissociation (ETD) shows their great utility and complementarity for t

134 dictions for electron transfer dissociation (ETD) spectra and HCD spectra of less abundant charges (1

135 hods such as electron transfer dissociation (ETD) tandem mass spectrometry (MS/MS) as well as in char

136 Moreover, electron transfer dissociation (ETD) tandem mass spectrometry (MS/MS) on a large myoglob

137 ed online by electron transfer dissociation (ETD) tandem mass spectrometry (MS/MS).

138 int HR-HRPF, electron-transfer dissociation (ETD) tandem MS (MS/MS) acquisition, measurement of effec

139 ly developed electron-transfer dissociation (ETD) technique.

140 ive top-down electron transfer dissociation (ETD) techniques are employed to study AS interaction wit

141 on (PQD) and electron transfer dissociation (ETD) techniques for confident and quantitative identific

142 loped method electron transfer dissociation (ETD) to characterize the human ES cell phosphoproteome.

143 ond, we used electron transfer dissociation (ETD) to partially dissociate disulfide bonds followed by

144 Electron transfer dissociation (ETD) was employed to fragment the heavily modified long

145 Electron-transfer dissociation (ETD) was then used to pinpoint the 12 occupied O-glycosy

146 C-MS/MS with electron transfer dissociation (ETD) was used to identify proteins from specific locatio

147 Electron transfer dissociation (ETD) was used to sequence bis-arylhydrazone (BAH)-cross-

148 rmation from electron-transfer dissociation (ETD) were observed, suggesting the utility of this metho

149 on (CID) and electron transfer dissociation (ETD) with hybrid quadrupole time-of-flight instruments a

150 achieved by electron-transfer dissociation (ETD) with increased supplemental activation, without los

151 mentation of electron transfer dissociation (ETD) with online CZE separations for top-down proteomics

152 or augmented electron transfer dissociation (ETD) yields.

153 on (CID) and electron-transfer dissociation (ETD)).

154 s our use of electron transfer dissociation (ETD), a mass spectrometric fragmentation technique that

155 Electron transfer dissociation (ETD), a technique that provides efficient fragmentation

156 ation (HCD), electron transfer dissociation (ETD), and electron capture dissociation (ECD).

157 ation (HCD), electron-transfer dissociation (ETD), and electron-transfer combined with higher-energy

158 tion (UVPD), electron-transfer dissociation (ETD), and electron-transfer/higher-energy collision diss

159 e CID (HCD), electron-transfer dissociation (ETD), and the combinations ETciD and EThcD.

160 followed by electron-transfer dissociation (ETD), chemical cross-linking, and molecular docking.

161 e CID (HCD), electron-transfer dissociation (ETD), ETciD, and EThcD.

162 tra, such as Electron Transfer Dissociation (ETD), Higher-energy Collisional Dissociation (HCD) spect

163 ion (CAD) or electron transfer dissociation (ETD), respectively.

164 line with an electron transfer dissociation (ETD)-enabled hybrid Orbitrap Fourier transform mass spec

165 ted a hybrid electron-transfer dissociation (ETD)-HCD acquisition protocol and developed a novel data

166 followed by electron transfer dissociation (ETD)-MS(2) upon detection of glycan-specific oxonium is

167 D-MS/MS) and electron transfer dissociation (ETD)-MS/MS of intercross-linked peptides (two peptides c

168 first use of electron transfer dissociation (ETD)-produced diagnostic fragment ions to probe the comp

169 on (CID) and electron transfer dissociation (ETD).

170 enabled with electron transfer dissociation (ETD).

171 25 kDa) with electron transfer dissociation (ETD).

172 (CID-MS(2)), electron-transfer dissociation (ETD-MS(2)), and CID of an isolated product ion derived f

173 d by precursor molecular weight and top-down ETD and HCD analysis of the reduced protein.

174 Native top-down ETD on the protein-ligand complexes shows how the differ

175 nd comparable sequence coverage for top-down ETD with orbitrap mass analyzers.

176 ns remain localized on basic residues during ETD but easily mobilize along the backbone during collis

177 electron capture/transfer dissociation (ECD, ETD) (c/z fragments), suggesting substantial contributio

178 ctron capture and transfer dissociation (ECD/ETD or ExD) mass spectrometry (MS) employed for peptide

179 ociation/electron transfer dissociation (ECD/ETD) product ions based on their number of hydrogen plus

180 electron capture/transfer dissociation (ECD/ETD).

181 ndergo dissociation analogous to that in ECD/ETD.

182 in the companion article, the number of ECD/ETD product ion amino acid compositions as a function of

183 discrimination between N- and C-terminal ECD/ETD peptides.

184 instruments not originally equipped with ECD/ETD capabilities.

185 e residues, which were overcome by employing ETD fragmentation.

186 ), a recently introduced method for enhanced ETD fragmentation, provides useful performance with CZE

187 was achieved using the instrument's existing ETD capabilities.

188 In fact, ETD can be performed with optimal efficiency in nearly t

189 radical anion of fluoranthene (m/z 202) for ETD and the closed-shell anion resulting from H atom att

190 ce of UniNovo is superior to other tools for ETD spectra and superior or comparable with others for C

191 CID of an isolated product ion derived from ETD (MS(3)) has been used to characterize disulfide-link

192 degludec/liraglutide vs 1.8 kg for glargine; ETD, -3.20 kg [95% CI, -3.77 to -2.64],P < .001) and few

193 rits of our method in analyzing glycopeptide ETD data.

194 is necessary for interpreting O-glycopeptide ETD spectra in order to expedite the analysis workflow.

195 tested types of spectra (including CID, HCD, ETD, CID/ETD and HCD/ETD spectra of trypsin, LysC or Asp

196 ra (including CID, HCD, ETD, CID/ETD and HCD/ETD spectra of trypsin, LysC or AspN digested peptides).

197 HDX-ETD offers some binding-site characterization with amino

198 esponsible for the observed differences (HDX-ETD).

199 rk highlight the analytical potential of HDX-ETD combined with functional assays to guide protein eng

200 Gas-phase HDX-ETD experiments on ubiquitin ions ionized from both dena

201 identified 50% more peptides than ETD; (ii) ETD resulted in approximately 20% increase in amino acid

202 Mate was used to reinfuse samples to improve ETD and HCD spectra of glycopeptides.

203 erred over peptide backbone fragmentation in ETD.

204 died the fragmentation of O-glycopeptides in ETD and found useful rules that facilitate their identif

205 ince the glycan side chain remains intact in ETD, and the glycosylation site can be localized on the

206 The relative high abundance ions observed in ETD provided strong evidence for the linked peptide info

207 work with any search engine that interprets ETD data of N-linked glycopeptides.

208 ciation (ETD) reactions, i.e., activated ion ETD (AI-ETD), significantly increases dissociation effic

209 HbA(1c) than both subcutaneous liraglutide (ETD -0.2%, 95% CI -0.3 to -0.1; p=0.0056) and placebo (E

210 this method for improving transmission mode ETD performance for relatively low charge states of pept

211 r dissociation-selected reaction monitoring (ETD-SRM) was developed to investigate isoAsp sites in MU

212 from a single protein precursor or multiple ETD/proton-transfer reactions (PTR) reaction periods.

213 ort that negative ion CAD, EDD, and negative ETD (NETD) result in sulfonate retention mainly at highe

214 On average, 58% of the observed ETD product ion abundance was accounted for by fragment

215 re, we present a study on the application of ETD for analysis of phospholysine (pLys) peptides.

216 d highlight the different characteristics of ETD spectra of doubly charged precursors in comparison t

217 data, due to the complexity and diversity of ETD mass spectra compared to CID counterparts.

218 e to construct and enables implementation of ETD on any instrument without modification to footprint.

219 been studied for decades, the intricacies of ETD-based fragmentation have not yet been firmly establi

220 Because of the nature of ETD fragmentation MS experiments were performed without

221 Upon optimization of ETD and product ion transfer parameters, results show th

222 n to the preferred fragmentation pathways of ETD, the TMT-127 and -129 reagents were recently modifie

223 he parameters governing the reaction rate of ETD are examined experimentally.

224 h that takes advantage of the specificity of ETD and the scalability of tandem MS, and the predictive

225 oughput glycopeptide identification based on ETD data, due to the complexity and diversity of ETD mas

226 ch was greater than by using CAD (38,293) or ETD (39,507) alone.

227 Compared with the Ascore using either CAD or ETD, the Cscore identified up to 88% more phosphorylatio

228 Typical ECD or ETD backbone fragmentations are completely inhibited in

229 upts the normal sequence of events in ECD or ETD, leading to backbone fragmentation by forming a stab

230 e tags generated either by low-energy HCD or ETD activation along with the intact protein mass inform

231 her fragmentation spectra compared to HCD or ETD alone, increasing protein sequence coverage, and the

232 -type ions observed when compared to UVPD or ETD alone, as well as generating a more balanced distrib

233 oving peptide identifications over all other ETD methods, making it a valuable new tool for hybrid fr

234 eptide fragmentation and identification over ETD and other supplemental activation methods.

235 type product ions for all charge states over ETD alone, boosting product ion yield by nearly 4-fold f

236 n to the original HCD-product dependent (pd)-ETD function.

237 te a drastic reduction in the time taken per ETD event.

238 95% CI -0.3 to -0.1; p=0.0056) and placebo (ETD -1.2%, -1.4 to -1.0; p<0.0001) at week 26.

239 95% CI -2.2 to -0.9; p<0.0001) and placebo (ETD -4.0 kg, -4.8 to -3.2; p<0.0001).

240 3 to 0.0; p<0.0001) and superior to placebo (ETD -1.1%, -1.2 to -0.9; p<0.0001) by use of the treatme

241 ained ETD and ECD models are able to predict ETD and ECD spectra with reasonable accuracy in ion inte

242 In such cases, good quality ETD data may indicate the discrepancy, and will also dis

243 The resultant ETD and CID spectra were used for the identification of

244 e other hand, homologous murine rotaviruses (ETD or EHP) or the heterologous simian rotavirus (rhesus

245 and PTM localization by combining sequential ETD and HCD fragmentation in a single fragmentation even

246 mass spectrometers, ExD, more specifically, ETD MS has received particular interest in life science

247 nsfer dissociation tandem mass spectrometry (ETD MS/MS) has been demonstrated in both model peptides

248 s limitation, here we report an improved SPS/ETD workflow for TMT-based intact glycopeptide quantific

249 g a glycoprotein interference model, the SPS/ETD approach was demonstrated to offer improved accuracy

250 The SPS/ETD approach was implemented on an Orbitrap Tribrid mass

251 e than triples identifications from standard ETD experiments and outperforms ETcaD and EThcD as well.

252 y between fragments generated using standard ETD with a short reaction time and AI-ETD with a long re

253 at (i) CID identified 50% more peptides than ETD; (ii) ETD resulted in approximately 20% increase in

254 From these experiments, we conclude that ETD identifies a larger number of unique phosphopeptides

255 A central finding is that ETD is approximately 2-fold more likely to cleave in sol

256 Here we show that ETD can be combined with fast gas-phase HDX in ND(3) gas

257 The ETD model was trained with more than 7000 ETD spectra, w

258 target glycopeptides, are scored against the ETD data, from which FDRs can be calculated accurately b

259 ins, phosphate transfer reactions during the ETD process can be observed leading to ambiguous fragmen

260 ms the cleavage of the disulfides during the ETD process.

261 lation sites were clearly localized from the ETD fragmentation data.

262 hown to be obtained either directly from the ETD fragmentation of the precursors (disulfide-linked pe

263 ally disulfide-dissociated peptides from the ETD fragmentation was necessary for linkage assignment.

264 From the ETD/HCD mass spectra, 5162 and 6720 reliable SNLs and im

265 ectly from the charge-reduced species in the ETD fragmentation of the precursors.

266 In this paper we investigate the ETD fragmentation patterns of peptides labeled with 8-pl

267 nfrared photon bombardment concurrent to the ETD reaction to mitigate nondissociative electron transf

268 tes input glycopeptide compositions with the ETD spectra, and assigns a score for each candidate.

269 The glycan remains intact through ETD, while the peptide backbone is cleaved, providing th

270 ion events as well as longer reaction times, ETD spectra require significantly more time to acquire t

271 n and alkylation of disulfide bonds prior to ETD analysis is evaluated by comparison of three derivat

272 Similarly to ETD, proton transfer is found to compete with electron t

273 are preferentially cleaved when subjected to ETD.

274 The trained ETD and ECD models are able to predict ETD and ECD spect

275 ssessing an electron available for transfer (ETD).

276 cleaved over the glycosidic cleavages under ETD fragmentation.

277 ce the products of reactions occurring under ETD conditions and provides insights into the parameters

278 Basically, pLys is stable under ETD conditions allowing an unambiguous assignment of the

279 and sequence coverage for all peptides upon ETD, including formation of diagnostic ions that allow c

280 product ions in multiple charge states upon ETD is minimized for the APTA-modified peptides.

281 We demonstrate that upon ETD, peptides labeled with 8-plex iTRAQ tags fragment to

282 , top-down proteomics can be performed using ETD in a linear ion trap mass spectrometer on a chromato

283 d demonstrate MS(2)-based quantitation using ETD.

284 ed peptide radical cations was studied using ETD.

285 the dynamic range of tandem MS analyses when ETD-based methods are compared to CID-based methods.

286 rison to the analogous MS(3) format in which ETD and UVPD were undertaken in separate segments of the

287 n-induced dissociation (CID) steps, in which ETD fragmentation preferentially induces cleavage of dis

288 efinitively extends the m/z range over which ETD can be effective for fragmentation of intact protein

289 While ETD retains modifications and cleaves disulfide bonds-ma

290 With ETD, labile glycans were retained without any signs of c

291 rly double the identifications achieved with ETD alone but also that it outperforms the other availab

292 pping capabilities of the LTQ, combined with ETD, are demonstrated to provide single-residue resoluti

293 emonstrate that CZE is fully compatible with ETD as well as higher energy collisional dissociation (H

294 ional linear ion trap volume concurrent with ETD reactions to limit uninformative nondissociative eve

295 Also, newly added modules deal with ETD/ECD analysis, multimodal mass spectra analysis, and

296 ity on a high-resolution Q-TOF equipped with ETD and an electrospray ionization interface consisting

297 The online LC-MS with ETD methodology represents a powerful approach to aid in

298 (XICs) of cysteine-containing peptides with ETD analysis to produce an efficient assignment approach

299 ciation data into the database searches with ETD data may prove decisive in most cases.

300 ss tag (TMT) labeling and an LTQ Orbitrap XL ETD (electron transfer dissociation) hybrid mass spectro