ライフサイエンスコーパス: Edman degradation

1 ther characterized by mass spectrometry, and Edman degradation.

2 e location of the cut sites through standard Edman degradation.

3 The attachment site was confirmed by Edman degradation.

4 phosphopeptides were subjected to sequential Edman degradation.

5 sis; of these, only 10 could be sequenced by Edman degradation.

6 mation whereby the N terminus was blocked to Edman degradation.

7 minus of the 38-kDa candidate was blocked to Edman degradation.

8 ated by reverse phase HPLC and identified by Edman degradation.

9 jected to N-terminal sequencing by automated Edman degradation.

10 no acid removal is a classic reaction termed Edman degradation.

11 , and labeled amino acids were identified by Edman degradation.

12 ads and analyzed using mass spectrometry and Edman degradation.

13 ons sequentially identified via conventional Edman degradation.

14 matography, mass spectrometric analysis, and Edman degradation.

15 ate; the N-terminus of ZP2 was identified by Edman degradation.

16 annel domain and in the ACh binding sites by Edman degradation.

17 entified that were purified and sequenced by Edman degradation.

18 mammals and hence blocked from sequencing by Edman degradation.

19 , and labeled amino acids were identified by Edman degradation.

20 ositive beads can be sequenced directly with Edman degradation.

21 same as the published sequence determined by Edman degradation.

22 ng from cleavage is blocked and resistant to Edman degradation.

23 c phosphopeptides, MALDI-TOF/MS analysis and Edman degradation.

24 te and its identity determined by sequential Edman degradation.

25 orthophthalaldehyde (OPA) prior to automated Edman degradation.

26 subjected to phosphoamino acid analysis and Edman degradation.

27 rminal pyroglutamyl residue that had blocked Edman degradation.

28 N-terminal Edman degradation amino acid sequence analysis showed th

29 n digestion, lectin affinity chromatography, Edman degradation amino acid sequence analysis, carbohyd

30 vitro, of which three could be identified by Edman degradation amino-acid sequencing.

31 Edman degradation analyses and co-migration of synthetic

32 Mass spectrometry and Edman degradation analyses show that pp alpha-GlcNAc-T2

33 is on an isolated glycated peptide utilizing Edman degradation analysis and MALDI-TOF/TOF mass spectr

34 Furthermore, Edman degradation analysis demonstrated that preferred s

35 Edman degradation analysis of a beta-arrestin 1 C-termin

36 Edman degradation analysis of aggregated proteins from t

37 Edman degradation analysis of collagen fiber degradation

38 no acid sequence was determined by automated Edman degradation analysis of proteolytic fragments of b

39 ains the original amino acid as confirmed by Edman degradation analysis, suggesting that the mRNA but

40 both Asp and isoAsp, which were assigned by Edman degradation and by isoAsp detection using protein

41 NH2-terminal sequences were determined by Edman degradation and compared with the genomic sequence

42 amino acid sequence has been established by Edman degradation and confirmed by PCR analysis.

43 -terminal residues of each mature protein by Edman degradation and confirmed the internal deletion in

44 ion of the protein, based on blank cycles in Edman degradation and corresponding serine or threonine

45 dioactive lysine residues were identified by Edman degradation and electrospray mass spectrometry fol

46 Edman degradation and liquid chromatography of tryptic p

47 igestion of reduced derivatives, followed by Edman degradation and mass analyses.

48 Edman degradation and mass spectrometric analyses of try

49 olated and individually sequenced by partial Edman degradation and mass spectrometry (PED-MS) to reve

50 ation on the purified fraction, by automatic Edman degradation and mass spectrometry analysis, identi

51 Edman degradation and mass spectrometry of V8 protease g

52 Sequencing of the toxin by Edman degradation and mass spectrometry revealed a 63 am

53 Analysis of COOH-terminal peptides with Edman degradation and mass spectrometry revealed an amid

54 Sequencing of peptide fragments from MtmB by Edman degradation and mass spectrometry revealed no chan

55 P and DPP, we performed a detailed analysis (Edman degradation and mass spectrometry) on selected try

56 Purified peptides were sequenced by Edman degradation and mass spectrometry, and the sequenc

57 ified and peptides were sequenced by partial Edman degradation and mass spectrometry.

58 KPPHQGPRPPRPRPKP) was determined by means of Edman degradation and mass spectrometry.

59 , and the peptides were sequenced by partial Edman degradation and matrix-assisted laser desorption i

60 s determined to be LLNEVMCYPLFDGGNIGLR using Edman degradation and matrix-assisted laser desorption/i

61 of the labeled fragment, followed by manual Edman degradation and radiochemical sequencing.

62 (3-BP) blocks the amino terminus of 4-OT to Edman degradation and results in the disappearance of th

63 ified proteins were determined by sequential Edman degradation and tandem mass spectrometry (MS/MS).

64 ENAL, where X represents unknown residue) by Edman degradation, and a full-length cDNA of the enzyme

65 plex was characterized by mass spectrometry, Edman degradation, and amino acid composition analyses.

66 ing a combination of phosphopeptide mapping, Edman degradation, and electrospray mass spectrometry, s

67 er band was purified, partially sequenced by Edman degradation, and found to match rat IgG heavy chai

68 ucts by high pressure liquid chromatography, Edman degradation, and mass spectrometry suggests that m

69 ells using a combination of peptide mapping, Edman degradation, and mass spectrometry.

70 ion analysis and exoglycosidase digestions), Edman degradation, and monosaccharide composition analys

71 conjunction with phosphoamino acid analysis, Edman degradation, and phosphopeptide mapping, demonstra

72 The final candidate was also blocked to Edman degradation; as before, a duplex probe was PCR amp

73 ions, with analysis by mass spectrometry and Edman degradation, both the heavy and light chains were

74 horesis (PAGE), Western immunoblot analysis, Edman degradation, circular dichroism spectroscopy, and

75 nal sequence of the MalH protein obtained by Edman degradation corresponded to the first 32 amino aci

76 Edman degradation demonstrated a phosphotyrosine in a YX

77 Characterization by mass spectrometry and Edman degradation demonstrated that both the N and C ter

78 sequence EEDRD and so forth as determined by Edman degradation, demonstrating signal peptidase proces

79 with oligonucleic acids, which differs from Edman degradation due to their inherent sensitivity to a

80 Edman degradation (ED) has been used for primary sequenc

81 was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrom

82 become phosphorylated; sequence analysis by Edman degradation established that threonine 753 became

83 Edman degradation failed to reveal N-terminal sequences,

84 Automated Edman degradation for sequencing is the classic tool for

85 Edman degradation gave a single amino-terminal sequence

86 nt both by ion trap mass spectrometry and by Edman degradation identified Asn346 and Asn347 of alphaO

87 N-terminal sequencing by Edman degradation identified residue 16 as carboxymethyl

88 Partial proteolytic mapping and Edman degradation identified serine 257 as a major site

89 ides by automated microsequencing and manual Edman degradation identified the sites in Mnk1 as Thr(22

90 SDS-PAGE analysis followed by Edman degradation identified three cysteine-containing f

91 SDS/PAGE, mass spectrometry, and Edman degradation identified translation products corres

92 pt cysteine 211 was identified by sequential Edman degradation, implying that this was the amino acid

93 all, although our method does not outperform Edman degradation in efficiency, it serves as a valuable

94 OOH-terminal fragment yielded no sequence by Edman degradation, indicating that parts of Ser-180 went

95 usly the frameshift through a combination of Edman degradation, MALDI-ToF mass fingerprint analysis o

96 ing peptides inside the bead using a partial Edman degradation/mass spectrometry method.

97 encoding peptides inside the bead by partial Edman degradation/mass spectrometry.

98 primary sequence was determined by combining Edman degradation/N-terminal sequencing and electrospray

99 Edman degradation of 32P-labeled protein identified seri

100 combination of tandem mass spectrometry and Edman degradation of a subfragment.

101 aphy and obtained its amino acid sequence by Edman degradation of a tryptic digest.

102 Solid-phase Edman degradation of I was used for positive identificat

103 sidues in each M4 segment were identified by Edman degradation of isolated tryptic fragments and gene

104 the agonist binding sites were identified by Edman degradation of isolated, labeled subunit fragments

105 Sequential cycles of Edman degradation of labeled receptor fragments identifi

106 Edman degradation of MTPv1 isolated from transfected cel

107 structure of CP2 was determined by automated Edman degradation of native CP2 and its proteolytic frag

108 n of tandem mass spectroscopy and N-terminal Edman degradation of peptide fragments after a series of

109 ation along with data derived from automatic Edman degradation of peptide fragments, the SmCI sequenc

110 d Lys-92 and Lys-109 as acetylation sites by Edman degradation of peptides from [14C]acetate-labeled

111 Mapping, isolation, and Edman degradation of the ATP-protectable peptide from [3

112 Edman degradation of the F1 subunit yielded the sequence

113 Sequencing by Edman degradation of the intact polypeptides and mass sp

114 Phosphoamino acid analysis and manual Edman degradation of the isolated phosphopeptides enable

115 Edman degradation of the larger amelogenin ran for 42 cy

116 e determined for the N terminus by automated Edman degradation of the purified enzyme.

117 sequence of halocin S8 was obtained first by Edman degradation of the purified protein and verified f

118 Edman degradation of the purified protein determined the

119 attachment to the protein was determined by Edman degradation of the resulting peptide-DNA complex t

120 Amino acid analysis and Edman degradation of tryptic peptides proved that the co

121 lectrospray ionization-mass spectrometry and Edman-degradation of peptides derived from HNE-modified

122 nce analysis; in these cases, sequencing (by Edman degradation or by mass spectrometry) confirmed tha

123 were subjected to multiple cycles of partial Edman degradation (PED) by the treatment with a 15-30:1

124 rated by reversed-phase HPLC for analysis by Edman degradation peptide sequencing.

125 eling with [(32)P]H(3)PO(4), modified manual Edman degradation, phosphoamino acid analysis, endoprote

126 site is evident in desleucyl-oritavancin, an Edman degradation product of oritavancin, still retainin

127 ns is important for amino acid sequencing by Edman degradation, protein identification by shotgun and

128 on of [(35)S]methionine residues released by Edman degradation reaction.

129 Edman degradation remains the primary method for determi

130 Sequencing by Edman degradation revealed a 21-residue peptide (GCRFCCN

131 Edman degradation revealed that each mutant protein was

132 Amino acid sequence analysis by Edman degradation revealed that it has 47 residues, with

133 e sequence analysis by mass spectrometry and Edman degradation revealed that RH70 is the previously r

134 Edman degradation revealed that Trp-6 and Trp-9 were cov

135 phosphoserine was defined by the N-terminal Edman degradation sequence analysis as being the fourth

136 amino acid composition analysis, N-terminal Edman degradation sequence analysis, and tandem mass spe

137 eins and confirmed MMP-dependent cleavage by Edman degradation sequence analysis.

138 s could not be identified by analysis of the Edman degradation sequencer product because the palmitoy

139 confirmed by mass spectrometry analysis and Edman degradation sequencing of proteolytic products gen

140 Edman degradation sequencing of radiolabeled cyanogen br

141 ture for these peptides was determined using Edman degradation sequencing, and their cystine pairing

142 h transmembrane domain), as proven by direct Edman degradation sequencing.

143 ing, deglycosylation, micropurification, and Edman degradation sequencing.

144 f a tryptic peptide by mass spectrometry and Edman degradation showed a cleavage after Val129.

145 teine 8, combined with mass spectrometry and Edman degradation, showed that disulfide bonds link cyst

146 rgan with [(14)C]halothane and determined by Edman degradation some of the photolabeled amino acids i

147 cing proteins, such as mass spectrometry and Edman degradation, suffer from short reads and lack sens

148 Edman degradation suggested that the radiolabeled phosph

149 Specialized Edman degradation techniques have completed the structur

150 sequence of the precursor, we determined by Edman degradation the N-terminal amino acid sequences of

151 hRs with [(3)H]epibatidine and identified by Edman degradation the photolabeled amino acids.

152 Following Edman degradation, three 17-mer oligodeoxyribonucleotide

153 ecreted prorenin was determined by automated Edman degradation to be Leu22 while the N-terminus of th

154 tide fragments, which were then sequenced by Edman degradation to determine the glycosylation sites.

155 was established, by mass spectroscopy and by Edman degradation, to be between a tryptophan at positio

156 An N-terminal block to Edman degradation was observed when any of five differen

157 Automated Edman degradation was used to obtain N-terminal and inte

158 Using a combination of mass spectrometry and Edman degradation, we mapped the cleavage sites and char

159 acid sequences of these proteins obtained by Edman degradation were compared with sequences from the

160 he wild-type or mutant peptides by automated Edman degradation were unsuccessful.

161 compatibility of "chemical linearization" by Edman degradation with a prominent macrocycle scaffold b

162 sequences from de novo mass spectrometry and Edman degradation with an expressed sequence tag library