ライフサイエンスコーパス: Edman

1 Edman analysis established their sequences as Val-Glu-As

2 Edman degradation (ED) has been used for primary sequenc

3 Edman degradation analyses and co-migration of synthetic

4 Edman degradation analysis of a beta-arrestin 1 C-termin

5 Edman degradation analysis of aggregated proteins from t

6 Edman degradation analysis of collagen fiber degradation

7 Edman degradation and liquid chromatography of tryptic p

8 Edman degradation and mass spectrometric analyses of try

9 Edman degradation and mass spectrometry of V8 protease g

10 Edman degradation demonstrated a phosphotyrosine in a YX

11 Edman degradation failed to reveal N-terminal sequences,

12 Edman degradation gave a single amino-terminal sequence

13 Edman degradation of 32P-labeled protein identified seri

14 Edman degradation of MTPv1 isolated from transfected cel

15 Edman degradation of the F1 subunit yielded the sequence

16 Edman degradation of the larger amelogenin ran for 42 cy

17 Edman degradation of the purified protein determined the

18 Edman degradation remains the primary method for determi

19 Edman degradation revealed that each mutant protein was

20 Edman degradation revealed that Trp-6 and Trp-9 were cov

21 Edman degradation sequencing of radiolabeled cyanogen br

22 Edman degradation suggested that the radiolabeled phosph

23 Edman microsequence analysis of the subunits after impor

24 Edman sequencing and mass spectrometry results indicated

25 Edman sequencing of cell-associated Iga determined that

26 Edman sequencing of class I peptide pools generates "mot

27 Edman sequencing of the lectin-positive bands gave the A

28 Edman sequencing revealed that two of the major caspase-

29 Edman sequencing was used to assign structure to subsite

30 Edman sequencing, mass spectrometry, ultraviolet-visible

31 site is evident in desleucyl-oritavancin, an Edman degradation product of oritavancin, still retainin

32 P and DPP, we performed a detailed analysis (Edman degradation and mass spectrometry) on selected try

33 conjunction with phosphoamino acid analysis, Edman degradation, and phosphopeptide mapping, demonstra

34 horesis (PAGE), Western immunoblot analysis, Edman degradation, circular dichroism spectroscopy, and

35 tic digestion of the heme-protein adduct and Edman sequencing and mass spectrometric analysis of the

36 Amino acid analysis and Edman degradation of tryptic peptides proved that the co

37 confirmed by mass spectrometry analysis and Edman degradation sequencing of proteolytic products gen

38 c phosphopeptides, MALDI-TOF/MS analysis and Edman degradation.

39 subjected to phosphoamino acid analysis and Edman degradation.

40 matography, mass spectrometric analysis, and Edman degradation.

41 tion ionization time-of-flight analysis, and Edman sequencing, demonstrated that NADH attachment occu

42 alyzed by collision-induced dissociation and Edman sequencing of radiolabeled peptides.

43 Mapping, isolation, and Edman degradation of the ATP-protectable peptide from [3

44 Partial proteolytic mapping and Edman degradation identified serine 257 as a major site

45 ing, deglycosylation, micropurification, and Edman degradation sequencing.

46 determined, by a combination of HPLC/MS and Edman sequencing, the glycosylation sites in the extrace

47 ion site-specific manner using FT-ICR-MS and Edman sequencing.

48 th kinases, and through both mutagenesis and Edman phosphate ((32)P) release sequencing, established

49 Mass spectrometry and Edman degradation analyses show that pp alpha-GlcNAc-T2

50 Characterization by mass spectrometry and Edman degradation demonstrated that both the N and C ter

51 combination of tandem mass spectrometry and Edman degradation of a subfragment.

52 e sequence analysis by mass spectrometry and Edman degradation revealed that RH70 is the previously r

53 f a tryptic peptide by mass spectrometry and Edman degradation showed a cleavage after Val129.

54 sequences from de novo mass spectrometry and Edman degradation with an expressed sequence tag library

55 ions, with analysis by mass spectrometry and Edman degradation, both the heavy and light chains were

56 teine 8, combined with mass spectrometry and Edman degradation, showed that disulfide bonds link cyst

57 cing proteins, such as mass spectrometry and Edman degradation, suffer from short reads and lack sens

58 Using a combination of mass spectrometry and Edman degradation, we mapped the cleavage sites and char

59 ads and analyzed using mass spectrometry and Edman degradation.

60 ites, as determined by mass spectrometry and Edman sequencing analysis.

61 cation was obtained by mass spectrometry and Edman sequencing in recombinant mouse PrP secreted from

62 Moreover, using tandem mass spectrometry and Edman sequencing, specific intramolecular sites of inter

63 Using mass spectrometry and Edman sequencing, we have mapped additional phosphorylat

64 e determined by tandem mass spectrometry and Edman sequencing.

65 lectrospray ionization-mass spectrometry and Edman-degradation of peptides derived from HNE-modified

66 SDS/PAGE, mass spectrometry, and Edman degradation identified translation products corres

67 ther characterized by mass spectrometry, and Edman degradation.

68 mapping, electrospray mass spectrometry, and Edman protein sequencing.

69 tographic techniques, mass spectrometry, and Edman sequencing, a new hexapeptide (SSLSKL) from the Me

70 ectrospray ionization mass spectrometry, and Edman sequencing, we identified a single luciferase pept

71 Automated Edman degradation for sequencing is the classic tool for

72 Automated Edman degradation was used to obtain N-terminal and inte

73 without further purification to an automated Edman sequencer.

74 me-of-flight mass spectrometry and automated Edman sequencing analysis unequivocally identified the p

75 onstrated by successful manual and automated Edman sequencing.

76 er-727 using mass spectrometry and automated Edman sequencing.

77 no acid sequence was determined by automated Edman degradation analysis of proteolytic fragments of b

78 structure of CP2 was determined by automated Edman degradation of native CP2 and its proteolytic frag

79 e determined for the N terminus by automated Edman degradation of the purified enzyme.

80 ecreted prorenin was determined by automated Edman degradation to be Leu22 while the N-terminus of th

81 he wild-type or mutant peptides by automated Edman degradation were unsuccessful.

82 jected to N-terminal sequencing by automated Edman degradation.

83 ity of the protein was revealed by automated Edman microsequencing and a computer database search.

84 was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrom

85 orthophthalaldehyde (OPA) prior to automated Edman degradation.

86 ation on the purified fraction, by automatic Edman degradation and mass spectrometry analysis, identi

87 ation along with data derived from automatic Edman degradation of peptide fragments, the SmCI sequenc

88 It is now routine using automatic Edman microsequencing to determine the primary structure

89 rminal pyroglutamyl residue that had blocked Edman degradation.

90 rate analog covalently attached to Glu-68 by Edman sequencing and radioanalysis using C18 reverse pha

91 This was achieved by Edman amino acid sequencing of the isolated tandem repea

92 become phosphorylated; sequence analysis by Edman degradation established that threonine 753 became

93 rated by reversed-phase HPLC for analysis by Edman degradation peptide sequencing.

94 Amino acid sequence analysis by Edman degradation revealed that it has 47 residues, with

95 released from the membrane were analyzed by Edman sequencing.

96 liquid chromatography (HPLC) and analyzed by Edman sequencing.

97 nt both by ion trap mass spectrometry and by Edman degradation identified Asn346 and Asn347 of alphaO

98 was established, by mass spectroscopy and by Edman degradation, to be between a tryptophan at positio

99 both Asp and isoAsp, which were assigned by Edman degradation and by isoAsp detection using protein

100 affinity chromatography and characterized by Edman sequencing and mass spectrometry.

101 estion products of DGP were characterized by Edman sequencing and mass spectrometry.

102 eins and confirmed MMP-dependent cleavage by Edman degradation sequence analysis.

103 ains the original amino acid as confirmed by Edman degradation analysis, suggesting that the mRNA but

104 The attachment site was confirmed by Edman degradation.

105 versatility because it is not detectable by Edman sequencing and because it cannot be labeled with r

106 se amino acid preferences were determined by Edman amino acid sequencing.

107 NH2-terminal sequences were determined by Edman degradation and compared with the genomic sequence

108 attachment to the protein was determined by Edman degradation of the resulting peptide-DNA complex t

109 rgan with [(14)C]halothane and determined by Edman degradation some of the photolabeled amino acids i

110 sequence of the precursor, we determined by Edman degradation the N-terminal amino acid sequences of

111 sequence EEDRD and so forth as determined by Edman degradation, demonstrating signal peptidase proces

112 same as the published sequence determined by Edman degradation.

113 ary structure of MM-kappaI was determined by Edman sequence analysis and mass spectrometry.

114 acid sequence of this band was determined by Edman sequencing and mass spectroscopy.

115 The O-glycosylation site was determined by Edman sequencing of pronase-digested Ambn, which gave HP

116 amino acid sequence has been established by Edman degradation and confirmed by PCR analysis.

117 sequence of halocin S8 was obtained first by Edman degradation of the purified protein and verified f

118 igestion of reduced derivatives, followed by Edman degradation and mass analyses.

119 SDS-PAGE analysis followed by Edman degradation identified three cysteine-containing f

120 vitro, of which three could be identified by Edman degradation amino-acid sequencing.

121 dioactive lysine residues were identified by Edman degradation and electrospray mass spectrometry fol

122 sidues in each M4 segment were identified by Edman degradation of isolated tryptic fragments and gene

123 the agonist binding sites were identified by Edman degradation of isolated, labeled subunit fragments

124 hRs with [(3)H]epibatidine and identified by Edman degradation the photolabeled amino acids.

125 , and labeled amino acids were identified by Edman degradation.

126 ated by reverse phase HPLC and identified by Edman degradation.

127 , and labeled amino acids were identified by Edman degradation.

128 ate; the N-terminus of ZP2 was identified by Edman degradation.

129 acid sequence was verified independently by Edman analysis and/or electrospray ionization-mass spect

130 This derivative was confirmed indirectly by Edman analysis and more directly by mass spectrometry.

131 compatibility of "chemical linearization" by Edman degradation with a prominent macrocycle scaffold b

132 se-3 cleavage site of O-GlcNAcase, mapped by Edman sequencing, is a noncanonical recognition site tha

133 Sequencing of peptide fragments from MtmB by Edman degradation and mass spectrometry revealed no chan

134 Amino acid sequence obtained by Edman analysis of the AAB1 protein confirmed that the aa

135 nal sequence of the MalH protein obtained by Edman degradation corresponded to the first 32 amino aci

136 acid sequences of these proteins obtained by Edman degradation were compared with sequences from the

137 -terminal residues of each mature protein by Edman degradation and confirmed the internal deletion in

138 on of [(35)S]methionine residues released by Edman degradation reaction.

139 action, and identification of the residue by Edman sequencing.

140 ENAL, where X represents unknown residue) by Edman degradation, and a full-length cDNA of the enzyme

141 aphy and obtained its amino acid sequence by Edman degradation of a tryptic digest.

142 OOH-terminal fragment yielded no sequence by Edman degradation, indicating that parts of Ser-180 went

143 Purified peptides were sequenced by Edman degradation and mass spectrometry, and the sequenc

144 tide fragments, which were then sequenced by Edman degradation to determine the glycosylation sites.

145 er band was purified, partially sequenced by Edman degradation, and found to match rat IgG heavy chai

146 sis; of these, only 10 could be sequenced by Edman degradation.

147 entified that were purified and sequenced by Edman degradation.

148 N-terminal sequencing by Edman degradation identified residue 16 as carboxymethyl

149 Sequencing by Edman degradation of the intact polypeptides and mass sp

150 Sequencing by Edman degradation revealed a 21-residue peptide (GCRFCCN

151 ns is important for amino acid sequencing by Edman degradation, protein identification by shotgun and

152 mammals and hence blocked from sequencing by Edman degradation.

153 nce analysis; in these cases, sequencing (by Edman degradation or by mass spectrometry) confirmed tha

154 d Lys-92 and Lys-109 as acetylation sites by Edman degradation of peptides from [14C]acetate-labeled

155 annel domain and in the ACh binding sites by Edman degradation.

156 to identify protein phosphorylation sites by Edman sequencing of unseparated peptides.

157 to have the following primary structures by Edman microsequencing: IWLTALKFLGKHAAKHLAKQQLSKL-NH2 for

158 Sequencing of the toxin by Edman degradation and mass spectrometry revealed a 63 am

159 he primary structure of rPrP was verified by Edman sequencing and mass spectrometry, and secondary st

160 other Mel- strain (mel2), obtained from J.C. Edman (University of California at San Francisco, CA), p

161 n digestion, lectin affinity chromatography, Edman degradation amino acid sequence analysis, carbohyd

162 ucts by high pressure liquid chromatography, Edman degradation, and mass spectrometry suggests that m

163 00 fmol of an intact protein using classical Edman chemistry in combination with capillary-bore liqui

164 primary sequence was determined by combining Edman degradation/N-terminal sequencing and electrospray

165 ons sequentially identified via conventional Edman degradation.

166 We also describe Edman chemistry modifications that improve coupling effi

167 ion analysis and exoglycosidase digestions), Edman degradation, and monosaccharide composition analys

168 h transmembrane domain), as proven by direct Edman degradation sequencing.

169 named sublancin 168, and its behavior during Edman sequence analysis and its NMR spectrum suggested t

170 performance liquid chromatography and either Edman or mass spectrometric sequencing.

171 tosampler is used in combination with faster Edman cycles and a rapid 12-min PTH separation to signif

172 Following Edman degradation, three 17-mer oligodeoxyribonucleotide

173 osphoprotein and separating the peptides for Edman 32P-phosphate release sequencing.

174 nts, with sample cleanup and preparation for Edman sequencing performed using a commercial cartridge

175 tion, the PrcB N terminus is unavailable for Edman sequencing, suggesting that it is acylated.

176 with oligonucleic acids, which differs from Edman degradation due to their inherent sensitivity to a

177 also produces signature ions resulting from Edman cleavage and facilitates peptide sequencing on lin

178 Furthermore, Edman degradation analysis demonstrated that preferred s

179 We used peptide mapping by HPLC, Edman sequencing, and matrix-assisted time-of-flight (MA

180 E-reactive chicken proteins were identified (Edman, MS analysis) and quantified (ELISA).

181 imetric and chemiluminescent immunoblotting, Edman-based sequencing and mass spectrometry.

182 ion of the protein, based on blank cycles in Edman degradation and corresponding serine or threonine

183 ides by automated microsequencing and manual Edman degradation identified the sites in Mnk1 as Thr(22

184 Phosphoamino acid analysis and manual Edman degradation of the isolated phosphopeptides enable

185 ral rounds of HPLC, and gas-phase and manual Edman sequencing, is very tedious and requires about 10

186 of the labeled fragment, followed by manual Edman degradation and radiochemical sequencing.

187 eling with [(32)P]H(3)PO(4), modified manual Edman degradation, phosphoamino acid analysis, endoprote

188 ells using a combination of peptide mapping, Edman degradation, and mass spectrometry.

189 ing a combination of phosphopeptide mapping, Edman degradation, and electrospray mass spectrometry, s

190 We have developed a 20-min Edman cycle and a multiple sample horizontal flow reacto

191 uestion remains, "What is self?" Analyses of Edman motifs and of small sets of individual peptides su

192 usly the frameshift through a combination of Edman degradation, MALDI-ToF mass fingerprint analysis o

193 A combination of Edman sequence analysis and mass spectrometry identified

194 ation were determined using a combination of Edman sequencing and LC-MS.

195 Sequential cycles of Edman degradation of labeled receptor fragments identifi

196 KPPHQGPRPPRPRPKP) was determined by means of Edman degradation and mass spectrometry.

197 eptide library screening approaches based on Edman sequencing.

198 th subsequent mass spectrometric analysis or Edman sequencing.

199 all, although our method does not outperform Edman degradation in efficiency, it serves as a valuable

200 ing peptides inside the bead using a partial Edman degradation/mass spectrometry method.

201 olated and individually sequenced by partial Edman degradation and mass spectrometry (PED-MS) to reve

202 ified and peptides were sequenced by partial Edman degradation and mass spectrometry.

203 , and the peptides were sequenced by partial Edman degradation and matrix-assisted laser desorption i

204 encoding peptides inside the bead by partial Edman degradation/mass spectrometry.

205 were subjected to multiple cycles of partial Edman degradation (PED) by the treatment with a 15-30:1

206 P-like kinase, identified by "mixed-peptide" Edman sequencing after affinity purification, as the pre

207 yl isothiocyanate (PITC), promotes gas-phase Edman cleavage that yields abundant complementary b(1) a

208 Solid-phase Edman degradation of I was used for positive identificat

209 ge of the sequence and reports the predicted Edman cycles in which radioactivity would be observed if

210 sitivity of tagged analytes, while promoting Edman fragmentation and maintaining other sequence fragm

211 ified proteins were determined by sequential Edman degradation and tandem mass spectrometry (MS/MS).

212 pt cysteine 211 was identified by sequential Edman degradation, implying that this was the amino acid

213 te and its identity determined by sequential Edman degradation.

214 phosphopeptides were subjected to sequential Edman degradation.

215 Specialized Edman degradation techniques have completed the structur

216 plex was characterized by mass spectrometry, Edman degradation, and amino acid composition analyses.

217 e location of the cut sites through standard Edman degradation.

218 This provides "one-step Edman-like" information that, together with a fairly acc

219 on can then be used for other analyses, such Edman sequencing, amino acid analysis, or proteolytic di

220 no acid removal is a classic reaction termed Edman degradation.

221 nsional gels and subjected to NH(2)-terminal Edman sequencing for identification and determination of

222 enced by mass spectrometry and/or N-terminal Edman analysis.

223 N-terminal Edman degradation amino acid sequence analysis showed th

224 n of tandem mass spectroscopy and N-terminal Edman degradation of peptide fragments after a series of

225 phosphoserine was defined by the N-terminal Edman degradation sequence analysis as being the fourth

226 amino acid composition analysis, N-terminal Edman degradation sequence analysis, and tandem mass spe

227 xylesterase ES-10 (EC 3.1.1.1) by N-terminal Edman sequencing and extensive LC-MS/MS sequence analysi

228 We used N-terminal Edman sequencing and LC-MS/MS analysis to characterize t

229 pectrometry (nanoESI-QTOF MS) and N-terminal Edman sequencing for verifying connectivities.

230 Further, N-terminal Edman sequencing identified unique autocleavage sites in

231 erized using gel electrophoresis, N-terminal Edman sequencing, matrix-assisted laser desorption ioniz

232 mass spectrometry (ESI-q-TOF-MS), N-terminal Edman sequencing, peptide mapping, and other techniques.

233 grated approach, combining MS and N-terminal Edman sequencing, was capable of assigning the disulfide

234 Amino-terminal (Edman) sequence analysis of fragments derived from limit

235 to amino acid sequence analysis via both the Edman technique and mass spectroscopy.

236 s could not be identified by analysis of the Edman degradation sequencer product because the palmitoy

237 Collection of the Edman reaction fractional products reveals the radioacti

238 al amino acid sequence, determined using the Edman method (10-15 residues).

239 ine) will be available for reaction with the Edman reagent.

240 Through Edman sequencing and in vitro and in vivo biochemistry,

241 An N-terminal block to Edman degradation was observed when any of five differen

242 mation whereby the N terminus was blocked to Edman degradation.

243 minus of the 38-kDa candidate was blocked to Edman degradation.

244 The final candidate was also blocked to Edman degradation; as before, a duplex probe was PCR amp

245 (3-BP) blocks the amino terminus of 4-OT to Edman degradation and results in the disappearance of th

246 rger proteins, efficient deblocking prior to Edman sequencing is especially important to obtain quali

247 ng from cleavage is blocked and resistant to Edman degradation.

248 eparated, purified, and finally subjected to Edman microsequencing.

249 the tryptic peptides and subjecting them to Edman sequence analysis, the presence of repeating 3-hyd

250 Using Edman sequencing and mutagenesis, we identified a new an

251 Using Edman sequencing, 125I-SB206718 was shown to cross-link

252 Using Edman sequencing, we identified furin-type proteinase cl

253 Proteins were identified by using Edman sequencing, matrix-assisted laser desorption ioniz

254 ture for these peptides was determined using Edman degradation sequencing, and their cystine pairing

255 s determined to be LLNEVMCYPLFDGGNIGLR using Edman degradation and matrix-assisted laser desorption/i

256 Automated amino acid sequencing using Edman degradative chemistry of the repeat was used to de

257 equence of the truncated forms of TSLP using Edman protein sequencing and matrix-assisted laser desor

258 is on an isolated glycated peptide utilizing Edman degradation analysis and MALDI-TOF/TOF mass spectr

259 no-terminal cleavage site was identified via Edman sequencing.

260 ith phenylisothiocyanate in combination with Edman sequencing, and matrix-assisted laser desorption m

261 ositive beads can be sequenced directly with Edman degradation.

262 Analysis of COOH-terminal peptides with Edman degradation and mass spectrometry revealed an amid

263 N-terminal derivatization of peptides with Edman's reagent, phenyl isothiocyanate (PITC), promotes

264 andom doedecamer peptide library screen with Edman sequencing of MMP-20 cleavage products revealed th