ライフサイエンスコーパス: F plasmid

1 conjugative transfer of the Escherichia coli F plasmid.

2 structure of PsiB from the Escherichia coli F plasmid.

3 milar to the tra (conjugation) region of the F plasmid.

4 gation system related to that encoded by the F plasmid.

5 ested that this mechanism is confined to the F plasmid.

6 s the exclusion of T7 in cells harboring the F plasmid.

7 to part of the transfer (tra) operon of the F plasmid.

8 mids closely related to the Escherichia coli F plasmid.

9 netic elements like bacteriophage Mu and the F plasmid.

10 ell surface of Escherichia coli carrying the F plasmid.

11 opB protein involved in the equipartition of F plasmid.

12 nd the finO, traD, and repA sequences of the F-plasmid.

13 gly, just as seen for strains with lac on an F' plasmid.

14 equires that the lac operon be located on an F' plasmid.

15 am mutation in strain SM195 is carried on an F' plasmid.

16 y E. coli strains, 21 bacteriophage genomes, F plasmid and eight transposons.

17 ing features similar to those of TraA of the F plasmid and have shown that VirB2 is required for the

18 in, which is encoded by the Escherichia coli F plasmid and is involved in the partition of the single

19 the pCU1 relaxase is similar to that of the F plasmid and plasmid R388 relaxases.

20 ion group of six Tra proteins encoded by the F plasmid and required by F(+) cells to elaborate F pili

21 inimal origin region of the Escherichia coli F plasmid and the minimal origin of replication of the A

22 e pUTI89 plasmid has characteristics of both F plasmids and other known virulence plasmids.

23 In F-plasmid and related systems, the homologous protein ac

24 o be important for the stable inheritance of F-plasmids and the prophage form of bacteriophage P1.

25 the function of keeping a segregated pair of F plasmids apart while the cell septum is being formed.

26 , and Cuzin pioneered the development of the F plasmid as a model system to study replication control

27 We confirm this prediction for F plasmid as well as a distantly-related ParABS system.

28 (OMCC(F)) of a T4SS encoded by a conjugative F plasmid at <3.0 angstrom resolution by cryoelectron mi

29 The well-characterized F-plasmid-based CcdAB TA system is important for F-plasm

30 and the natural CcdB protein encoded by the F plasmid both inhibit bacterial growth by attacking DNA

31 nexpected parallels to the regulation of the F-plasmid CcdB activity by CcdA and further supports a c

32 Homologs of the F plasmid conjugation genes are physically located on th

33 patial patterns of gene transfer mediated by F plasmid conjugation in a colony of Escherichia coli gr

34 During F plasmid conjugation, however, the SOS response is supp

35 tructural and functional organization of the F plasmid conjugative coupling protein TraD by coimmunop

36 honates that are nanomolar inhibitors of the F plasmid conjugative relaxase in vitro.

37 Early in F plasmid conjugative transfer, the F relaxase, TraI, cl

38 An F-plasmid containing a ccd locus can, therefore, functio

39 From the measured number of F plasmid copies per cell it appears that each migrating

40 plasmid has a dual role in the partition of F plasmid copies to daughter cells prior to division.

41 e for structural transitions associated with F plasmid dissemination and F pilus biogenesis.

42 to prevent the redundant entry of additional F plasmids during active growth of the host cells.

43 Remarkably, F plasmids encode four distinct structures, not just the

44 overexpression of SopB, an Escherichia coli F plasmid-encoded partition protein, led to silencing of

45 TraI (DNA helicase I) is an Escherichia coli F plasmid-encoded protein required for bacterial conjuga

46 The iconic F plasmid-encoded T4SS has been central in understanding

47 ported in the 1940s, yet the architecture of F plasmid-encoded transfer channel and its physical rela

48 of gRNA into the host cytoplasm requires the F-plasmid-encoded coupling protein, TraD, which is locat

49 , the SOS response is suppressed by PsiB, an F-plasmid-encoded protein that binds and sequesters free

50 , facilitating proper folding of a subset of F-plasmid-encoded proteins in the periplasm.

51 The F-plasmid-encoded TraI protein, also known as DNA helica

52 um) interact with OmpW, and the prototypical F plasmid (Escherichia coli) interacts with OmpA.

53 The F plasmid genes examined were finO, traD, traY, and repA

54 The relatively high rate of recombination in F-plasmid genes suggests that conjugational gene transfe

55 The SopB protein of the F plasmid has a dual role in the partition of F plasmid

56 for quantifying the transfer kinetics of the F plasmid in a population by enumerating the relative ab

57 s studies have demonstrated that a DeltatrbB F plasmid in Escherichia coli lacking DsbC(E.coli), its

58 rus (PRV) genome was constructed as a stable F plasmid in Escherichia coli.

59 gative pili such as the conjugative pilus of F plasmid in Escherichia coli.

60 mixture experiments we observe an excess of F plasmid in the early saturation phase that equilibrate

61 assembled relaxosome encoded by the paradigm F plasmid in two different states corresponding to disti

62 s reversion of a lac frameshift allele on an F' plasmid in Escherichia coli.

63 The implications of F-plasmid insertion into the viral genome with regard to

64 However, the F-plasmid insertion present in the viral gG locus was fo

65 The insertion of the F-plasmid into the HHV8 genome interrupts the ORF56 gene

66 We find that F plasmid is actively brought to specific subcellular ho

67 ative pili, the F "sex" pilus encoded by the F plasmid is the best functionally characterized, and it

68 We have studied the interaction of the F plasmid killer protein CcdB with its intracellular tar

69 on of fatty acids, where the addition of the F-plasmid led to a reduction in cyclopropanation.

70 lowly dissociates from the 3'-end of cleaved F plasmid, likely a characteristic essential for plasmid

71 asmid-based CcdAB TA system is important for F-plasmid maintenance.

72 ally the most important, as the discovery of F-plasmid-mediated conjugation ushered in the era of mol

73 d the processing of the propilin TraA of the F plasmid now extends to E. coli.

74 epA, which map at dispersed positions on the F plasmid of E. coli.

75 s of F-pili (conjugative pili encoded by the F plasmid of Escherichia coli).

76 on plasmid QpH1 of Coxiella burnetii and the F plasmid of Escherichia coli, respectively, are shown t

77 ases, virtually identical) to those found in F plasmids of E. coli natural isolates.

78 As previously observed, we find that F-plasmid ParA undergoes collective migrations ("flips")

79 One well-studied model is the F plasmid partition system, SopABC.

80 F-plasmid partition complexes containing ParB(F) and par

81 Here, we reconstituted the F-plasmid partition system using a DNA-carpeted flow cel

82 Here we demonstrate that the F-plasmid-partitioning protein SopA polymerizes into fil

83 A derivative of the F plasmid, pOX38-tra715, expresses the entire F tra oper

84 ion allow phage T7 to avoid exclusion by the F plasmid, presumably by protecting the cell from premat

85 The F plasmid PsiB protein inhibits all activities of the Re

86 Here we report a mutagenesis of the F plasmid relaxase gene traI using in-frame, 31-codon in

87 The F plasmid relaxase TraI (1,756 amino acids) is also a hi

88 protein on the formation and activity of the F plasmid relaxosome has been examined.

89 The conjugative transfer of bacterial F plasmids relies on TraM, a plasmid-encoded protein tha

90 equence of RepA is not homologous to that of F plasmid RepE, we found by using fold-recognition progr

91 the HHV8 complete genome onto a prokaryotic F-plasmid replicon which allows the propagation of the r

92 Segregation of the prototypical F plasmid requires the centromere-binding protein SopB,

93 Conjugative transfer of F plasmids residing in the Enterobacteriaceae was first

94 is of an E. coli strain with and without the F-plasmid revealed changes in cyclopropanation of fatty

95 dance of the virulence plasmid and all three F-plasmid sequences in subspecies I serovar Choleraesuis

96 on of the active partitioning systems of the F plasmid (sopABC) or RK2 (O(B1) incC korB) resulted in

97 binding of SopB to specific sites within the F plasmid sopC locus involves mainly the C-terminal regi

98 In the F plasmid system, two auxiliary proteins have roles in e

99 g if the same interactions were observed for F-plasmid T4SS proteins and when one interaction partner

100 and a 5' to 3' DNA helicase that unwinds the F plasmid to provide the single-stranded DNA that is tra

101 the ability of TraR, encoded on the episomal F' plasmid, to upregulate the sigma(E) extracytoplasmic

102 The product of the Escherichia coli F plasmid traI gene is required for DNA transfer via bac

103 The F plasmid TraI protein (DNA helicase I) plays an essenti

104 Crystal structures of the F plasmid TraI relaxase domain, with and without bound s

105 TraI36, a domain of F plasmid TraI that contains relaxase activity, binds a

106 For F plasmid, TraI, a relaxase or nickase, binds a single p

107 F-pilin precursor, propilin, involves three F plasmid transfer products: TraA, the propilin precurso

108 onstant inoculation densities, we extract an F plasmid transfer rate of 5 x 10(-10) (cells/mL . min)(

109 erefore, two TraI molecules are required for F plasmid transfer.

110 antisense sRNA and the traJ mRNA to control F plasmid transfer.

111 Here, we show that TraT from the pKpQIL and F plasmids (TraT(pKpQIL) and TraT(F)) exhibits plasmid s

112 We have examined the role of the F-plasmid TraV outer membrane lipoprotein in the assembl

113 ole of two internal cysteine residues of the F-plasmid TraV outer membrane lipoprotein.

114 We have examined the effect of the F plasmid TraY protein on tra gene expression in vivo.

115 F plasmids use surface exclusion to prevent the redundan

116 The F' plasmid, which carries lac, contributes by stimulatin