ライフサイエンスコーパス: GABP

1 GABP (also known as nuclear respiratory factor 2) is a n

2 GABP alpha and GABP beta 1 bind the insulin-response ele

3 GABP alpha and PU.1 competed with each other for binding

4 GABP alpha bound to the mNE ets site and, in turn, recru

5 GABP alpha immunoprecipitated from insulin-treated, 32P-

6 GABP also activates the CD18 promoter in Schneider cells

7 GABP also directly regulates Foxo3 and Pten and hence su

8 GABP binds DNA containing a single PEA3/Ets-binding site

9 GABP cooperated with c-Myb and C/EBP alpha to activate t

10 GABP could bind to the regulatory regions of Pax5 and Cd

11 GABP increased the activity of the mNE promoter sevenfol

12 GABP is a heteromeric transcription factor complex that

13 GABP is a heteromeric transcription factor complex which

14 GABP is an ets transcription factor that regulates genes

15 GABP is composed of two subunits, the Ets-related GABP-a

16 GABP is composed of two subunits; the GABPalpha subunit

17 GABP may be regulated by MAP kinase phosphorylation.

18 GABP thus directly links TERT promoter mutations to aber

19 GABP, also known as nuclear respiratory factor 2, regula

20 GABP, of which GABPA is a component, is known to regulat

21 GABP, which is present in human fibroblast nuclear extra

22 amily consensus sequences, which bound PU.1, GABP, and an Ets factor present in myeloid cell extracts

23 confirm the functional relevance of the -119 GABP-binding site in vivo.

24 urprisingly, neither coactivator binds NRF-2(GABP), a multisubunit transcriptional activator associat

25 spiratory factor 2/GA binding protein (NRF-2/GABP), and ying-yang protein 1 (YY1).

26 urther showed that pro-IL-16 is located in a GABP transcriptional complex bound to the Skp2 promoter.

27 The duplications recruit a GABP tetramer by virtue of the native ETS motif and its

28 epsilon subunit gene, and that it requires a GABP binding site within this region.

29 These systematic analyses revealed a GABP-controlled gene regulatory module that programs mul

30 -specific MuSK gene expression by activating GABP(alphabeta) transcription factors in endplate-associ

31 In addition, GABP is critically required for antigen-stimulated T-cel

32 mobility shift assay with antibodies against GABP and AP-1, respectively.

33 ial cells with small interfering RNA against GABP and SP1 resulted in a significant (approximately 50

34 A binding of GH4NE containing both Ets-1 and GABP and HeLa nuclear extracts devoid of Ets-1 but conta

35 transcription is regulated by both PU.1 and GABP in developing B cells.

36 The ets factors, PU.1 and GABP, bind to three ets sites in the CD18 promoter, whic

37 arin-Sepharose, we have shown that Ets-1 and GABP, which are MAP kinase substrates, co-purify with co

38 subset of genes controlled by PGC-1alpha and GABP is dysregulated in Duchenne muscular dystrophy (DMD

39 rate here that GABP DNA binding activity and GABP-dependent gene expression in 3T3 cells are inhibite

40 GABP alpha and GABP beta 1 bind the insulin-response element of the pro

41 Expression of GABP alpha and GABP beta 1 squelches insulin-increased prolactin gene e

42 ha, which mediates specific DNA binding, and GABP-beta, which forms heterodimers and heterotetramers

43 t consists of GABP alpha, an ets factor, and GABP beta, a Notch-related protein.

44 the cellular transcription factors LZIP and GABP and also plays an essential role in cell proliferat

45 data for the transcription factors NRSF and GABP.

46 Cotransfection of Sp- and GABP-deficient Drosophila SL-2 cells with the HPR1 promo

47 Co-transfection of Sp1 and GABP activates CD18 more than the sum of their individua

48 element of the prolactin promoter, and anti-GABP alpha and anti-GABP beta 1 antibodies supershift a

49 actin promoter, and anti-GABP alpha and anti-GABP beta 1 antibodies supershift a species seen with nu

50 As GABP is widely expressed, a strong TATA box thus allevia

51 al genes that were previously reported to be GABP targets.

52 Both GABP and PU.1 bound Ets sites in the Lbr promoter in vit

53 tially binds Ets-1, while the BTE binds both GABP and Ets-1.

54 analysis of Fas promoter revealed that both GABP- and AP-1-binding sites were required for initiatin

55 of C/EBPepsilon, and activities supported by GABP were greatly enhanced by either C/EBPepsilon or PU.

56 transactivation potential also impair the C1-GABP interaction, indicating that the C1 factor function

57 In committed B lineage cells, GABP can promote Il7r transcription in the absence of PU

58 In T cells, GABP has been demonstrated to regulate the gene expressi

59 cond transcription factor from myeloid cells-GABP-binds to the mNE ets site but strongly activates th

60 lear extracts devoid of Ets-1 but containing GABP, we were able to show that the EBS-RRE preferential

61 rimarily for the transcription factors CTCF, GABP, GATA2, E2F, STAT, and YY1.

62 dies show that endogenous, pituitary-derived GABP and Ets-1 bind to the BTE, whereas Ets-1 preferenti

63 cluding Bcl-2, Bcl-X(L), and Mcl-1 as direct GABP target genes, underlying its pivotal role in HSC su

64 ses determined the association of endogenous GABP as well as Sp3 proteins with the -727/-476 region o

65 Thus, our results establish GABP as both a transcriptional activator factor and as a

66 ddition to regulating IL-7Ralpha expression, GABP is critically required for TCR rearrangements and h

67 s to activate TERT, probably by facilitating GABP heterotetramer binding.

68 ns, which selectively recruit the ETS factor GABP to activate TERT, the significance of other variant

69 ntified the Ets-related transcription factor GABP in nuclear extracts from GH cells.

70 am mutant TERT promoter transcription factor GABP, are being considered as promising therapeutic targ

71 ED1 and the Ets-related transcription factor GABP.

72 and GA-binding protein transcription factor (GABP-alpha) to their respective activator protein 1 (AP1

73 sults suggest that the transcription factors GABP and AP-1 play a critical role in the induction of F

74 Sp1 activated the distal enhancer 5-fold, GABP 3-fold, and the combination 8-fold in Schneider cel

75 PU.1 binding site and for PU.1, but not for GABP.

76 GABPalpha cDNA sequence including sites for GABP (-86, -104, -169, -257, and -994), YY1 (-57), Sp1 (

77 d within a 400 bp sequence and contains four GABP binding sites, a Sp1/3 binding site and an YY1 bind

78 Furthermore, GABP activates transcription of DNA methyltransferases a

79 leading algorithms using H3K27me3, H3K36me3, GABP, ESR1 and FOXA ChIP-seq datasets.

80 ome-wide GABP-binding site analyses identify GABP direct targets encoding proteins involved in cellul

81 termine the roles of individual cysteines in GABP redox regulation, we generated a series of serine s

82 tein complex--an enhanceosome--that includes GABP, other transcription factors, and coactivators, dyn

83 sulfhydryl-alkylating agents also inhibited GABP DNA binding activity.

84 and by thioredoxin; however, GSSG inhibited GABP DNA binding activity.

85 Instead, GABP provided transcriptional activation through the Lbr

86 Treatment of GABP-alpha, but not GABP-beta1, with sulfhydryl-alkylating agents also inhib

87 ion-associated factors, including SRF, NRSF, GABP, Stat3 and p300 in different developmental contexts

88 ases might directly regulate the activity of GABP, we studied MAP kinase-catalyzed and NRG-1-induced

89 ts, which were proportional to the amount of GABP added, required both the GABP alpha subunit and eit

90 cell activation increased the DNA binding of GABP and AP-1 to this enhancer site.

91 Binding of GABP to the GAA/CF6 bi-directional promoter provides the

92 The binding of GABP to the rpS16 initiation region does not significant

93 1 factor functions as a novel coactivator of GABP-mediated transcription.

94 ranscription factor complex that consists of GABP alpha, an ets factor, and GABP beta, a Notch-relate

95 Finally, small interfering RNA depletion of GABP in GH3 cells results in the loss of prolactin prote

96 kinase D2 (PRKD2) as a potential effector of GABP in HSCs.

97 Expression of GABP alpha and GABP beta 1 squelches insulin-increased p

98 Forced expression of GABP significantly enhanced R-ras mRNA expression level

99 ha expression and confirms the importance of GABP in the coordinate expression of respiratory chain c

100 d cells shows substantial phosphorylation of GABP alpha.

101 There was no increase in phosphorylation of GABP beta in response to insulin.

102 sing the possibility that phosphorylation of GABP by MAP kinases induces transcription of AChR genes.

103 Allelic recruitment of GABP is consistently observed across four cancer types,

104 The regulation of GABP (nuclear respiratory factor 2) DNA binding activity

105 To study the role of GABP at neuromuscular synapses, we conditionally inactiv

106 To examine the role of GABP in myeloid differentiation, we generated mice in wh

107 The specificity of GABP and AP-1 binding was demonstrated by competition el

108 1 (54 kDa) and/or the DNA binding subunit of GABP, GABPalpha (57 kDa).

109 ion of GABPalpha, the DNA-binding subunit of GABP, leads to early embryonic lethality, preventing ana

110 at GABPalpha, the ets DNA-binding subunit of GABP, physically interacts with p300 in myeloid cells.

111 ncy of GABPalpha, the DNA-binding subunit of GABP, resulted in profoundly defective B cell developmen

112 Although expression of rb, a target of GABP, is elevated in muscle tissue deficient in GABPalph

113 Treatment of GABP-alpha, but not GABP-beta1, with sulfhydryl-alkylati

114 expression vector encoding the Sp1, Sp3, or GABP gene induced luciferase gene expression.

115 ites functioned cooperatively with the other GABP binding sites and with the Sp1/3 and YY1 sites to p

116 The other GABP sites and the Sp1/3 and YY1 binding sites were func

117 ed from control cells does not phosphorylate GABP alpha while MAP kinase immunoprecipitated from insu

118 Potential GABP-binding sites are present in the promoters of numer

119 ription factors PU.1 and GA-binding protein (GABP) activate the Il7r promoter by interacting with a h

120 lation of PGC-1alpha and GA-binding protein (GABP) allows recruitment of PGC-1alpha to the GABP compl

121 f this complex to be the GA-binding protein (GABP) alpha.

122 erized the heterodimeric GA-binding protein (GABP) alphabeta complex and focused specifically on the

123 ontains highly conserved GA-binding protein (GABP) and YY1 binding sites, conferred high transcriptio

124 we demonstrate here that GA binding protein (GABP) bound to this site and was essential in the regula

125 GA binding protein (GABP) consists of GABPalpha and GABPbeta subunits.

126 ed by immunodepletion of GA-binding protein (GABP) from FM3A cell nuclear extracts.

127 ain transcription factor GA-binding protein (GABP) has been implicated to mediate synapse-specific ge

128 Although Ets-1 and GA binding protein (GABP) have been implicated in the Ras and insulin respon

129 studied the assembly of GA-binding protein (GABP) in solution and established the role of DNA in the

130 ily transcription factor GA-binding protein (GABP) in T cells impairs T-cell homeostasis.

131 ts) transcription factor GA binding protein (GABP) is a tetrameric transcription factor complex that

132 GA-binding protein (GABP) is a transcriptional regulator composed of two str

133 The GA-binding protein (GABP) is a ubiquitous heteromeric transcription factor i

134 The GA-repeat binding protein (GABP) is a ubiquitous transcription factor involved in t

135 GA binding protein (GABP) is a ubiquitously expressed Ets family transcripti

136 GA binding protein (GABP) is a ubiquitously expressed Ets-family transcripti

137 The transcription factor GA-binding protein (GABP) is composed of two subunits, GABPalpha and GABPbet

138 GA-binding protein (GABP) is the only Ets family transcription factor that f

139 nstructs encoding either GA-binding protein (GABP) or PU.1 inhibited Sp1-mediated promoter activation

140 2 and -682) containing a GA-binding protein (GABP) site and a low affinity activating protein-1 (AP-1

141 tions and found that the GA-binding protein (GABP) tetramer is responsible for promoter activation in

142 ate a binding site for a GA binding protein (GABP) transcription factor complex, whose assembly at th

143 itment of the multimeric GA-binding protein (GABP) transcription factor specifically to the mutant pr

144 and to the ERE sites by GA-binding protein (GABP) was confirmed by electrophoretic mobility shift as

145 The GA-binding protein (GABP), a heterodimeric transcription factor with widespr

146 ents the Pd from binding GA-binding protein (GABP), a transcription factor essential for Pd transcrip

147 ts transcription factor, GA-binding protein (GABP), and Sp1 were required for full RA responsiveness

148 n factor family protein, GA-binding protein (GABP), binds to the R-ras-derived sequence.

149 The transcription factor GA binding protein (GABP), consisting of DNA-binding subunit GABPalpha and t

150 TS transcription factor, GA-binding protein (GABP), with the relatively lineage-restricted E-twenty-s

151 Moreover, a GA-binding protein (GABP)-binding motif at -119 was necessary for mediating

152 factors such as Sp1 and GA-binding protein (GABP).

153 ric transcription factor GA-binding protein (GABP).

154 ember of the ETS family, GA binding protein (GABP).

155 mplexes, which contained GA-binding protein (GABP).

156 sites that are bound by GA-binding protein (GABP).

157 lly and functionally, as GA-binding protein (GABP)alpha/GABPbeta.

158 DNA binding of recombinant GABP-alpha was activated by chemical reduction (dithioth

159 to the mNE ets site and, in turn, recruited GABP beta to form a transcriptionally active complex.

160 have a dominant negative role in regulating GABP target genes.

161 is composed of two subunits, the Ets-related GABP-alpha, which mediates specific DNA binding, and GAB

162 In summary, serum-responsive GABP binding to Ets-binding sites activates the KIS prom

163 Mutations of the rpS16 GABP-binding sites that abolish binding increased rpS16

164 the more ubiquitously expressed factors Sp1, GABP alpha/beta, and NF2d9 are responsible for governing

165 tes for the human transcription factors SRF, GABP and NRSF at an average resolution of about 20 base

166 in vitro, whereas mutations that strengthen GABP binding caused a reduction in promoter activity.

167 The tetrameric GABP complex includes GABPalpha, which binds DNA via its

168 GH cells was phosphorylated 3-fold more than GABP alpha from control cells.

169 These findings demonstrate that GABP, PU.1, and C/EBPepsilon cooperate to control transc

170 We previously demonstrated that GABP is subject to redox regulation in vitro and in vivo

171 A-induced enhanceosome and demonstrates that GABP and p300 are essential components of CD18 RA respon

172 We demonstrate here that GABP binds to the rpS16 initiation region, and in so doi

173 We demonstrate here that GABP DNA binding activity and GABP-dependent gene expres

174 er studies, these new findings indicate that GABP can have a dual role as repressor or activator of r

175 These data indicate that GABP is dispensable for synapse-specific transcription a

176 These findings show that GABP has a nonredundant role in the control of T-cell ho

177 Our results suggest that GABP DNA binding activity is redox-regulated in vivo, po

178 These studies suggest that GABP mediates insulin-increased transcription of the pro

179 The GABP complex, consisting of DNA binding GABPalpha subuni

180 rlying network of auto-suppression among the GABP subunits.

181 tric analysis of the interaction between the GABP subunits determined an association constant (K(A))

182 the amount of GABP added, required both the GABP alpha subunit and either a beta1 or beta2 subunit.

183 the Fas enhancer, and mutation of either the GABP or AP-1 binding site severely reduced transcription

184 n such genes, e.g. the mouse rpL30 gene, the GABP-binding sites are located 40-80 base pairs upstream

185 ng selective pressure for recruitment of the GABP tetramer to activate TERT.

186 l-related kinase-dependent activation of the GABP(alpha/beta) transcription factor complex.

187 uction and by GSSG-mediated oxidation of the GABP-alpha subunit.

188 a provide strong support for the role of the GABP-binding motif in mediating Robo4 expression in the

189 cally ascribed to perturbed integrity of the GABP-controlled gene regulatory module in HSCs.

190 In addition, mutations that reduce the GABP transactivation potential also impair the C1-GABP i

191 In other studies, the GABP mutation was introduced into the endogenous mouse R

192 R-ras promoter sequence and suggest that the GABP may be critical for transcription of R-ras and for

193 ABP) allows recruitment of PGC-1alpha to the GABP complex and enhances transcription of a broad neuro

194 Robo4-LacZ transgenic cassette in which the GABP site was mutated.

195 Among these GABP-associated genes, knockdown of GABPalpha expression

196 Domains shared by the three GABP complexes, rather than solely the B1L tetramer, are

197 Thus, GABP binds to the crucial mNE promoter ets site and powe

198 Thus, GABP exists in solution as the heterodimer previously sh

199 Thus, GABP is a key regulator of B cell development, maturatio

200 Thus, GABP is essential for the regulation of IL-7Ralpha expre

201 Thus, GABP is regulated through at least two redox-sensitive a

202 Thus, GABP is required for HSC cell cycle entry and CML develo

203 sions were significantly smaller compared to GABP+/+ mice.

204 m it drives TERT maintenance via upregulated GABP dimers or a paralogous tetramer.

205 Whereas GABP operates as an essential upstream activator, PU.1 a

206 Transcriptome and genome-wide GABP-binding site analyses identify GABP direct targets

207 ollectively suggest that Sp1 cooperates with GABP to regulate HPR1 promoter activity.

208 Supplementation of cell-free extracts with GABP inhibits transcription on rpS16 templates while con

209 , a direct interaction of the C1 factor with GABP is demonstrated, defining the C1 factor as the crit

210 ctivator p300/CBP physically interacted with GABP in vivo, and p300 increased the responsiveness of t