ライフサイエンスコーパス: Gpp(NH)p

1 Gpp(NH)p together with sodium ion markedly decreased bin

2 GppNHp, a water molecule is hydrogen bonded to the side

3 tive affinities or amounts of high-affinity, Gpp(NH)p-sensitive binding of agonist.

4 -0.3 in remote myocardium; P<0.05), although GppNHp-stimulated cAMP production was equivocally reduce

5 TP or a commonly used nonhydrolyzable analog GppNHp (guanosine-5'-[(beta,gamma)-imido] triphosphate).

6 RalA bound to a non-hydrolyzable GTP analog (GppNHp) at 2.1 A resolution, providing the first structu

7 omplexes with a non-hydrolyzable GTP analog, GppNHp.

8 res of atlastin-1 bound to GDP.AlF(4)(-) and GppNHp, uncovering an intramolecular arginine finger tha

9 resembles other G proteins bound to GDP and GppNHp.

10 range and remarkably different for GDP- and GppNHp-bound forms of the G domain, indicating that the

11 Binding of GTPgammaS and GppNHp analogs to G(alphao) is of high affinity (K(D) 11

12 , fusion is still sensitive to GTPgammaS and GppNHp, indicating that there is a second specific GTPas

13 affinity peptides are released by both Arl2.GppNHp and Arl3.GppNHp, whereas the high affinity peptid

14 es are released by both Arl2.GppNHp and Arl3.GppNHp, whereas the high affinity peptides are exclusive

15 ptides are exclusively released by only Arl3.GppNHp.

16 r %R(H) (51% vs 44%) for beta(2)AR-G(s), but GppNHp failed to convert most of these to the low-affini

17 ased 4-fold by MgCl2 and increased 6-fold by Gpp(NH)p.

18 These effects were observed when either Gpp(NH)p was absent or present and they were comparable

19 PgammaS analogs (FL, 515, and TR), BODIPY FL GppNHp and BODIPY FL GTP molecules were synthesized as p

20 The affinities of BODIPY FL GppNHp for all four G(alpha) subunits are 10-fold lower

21 able GTP analog 5'-guanylylimidodiphosphate (Gpp(NH)p).

22 nce or presence of guanylylimidodiphosphate (Gpp(NH)p, 10[-5] M) to determine the percentage of 5-HT1

23 s of NGF tissue to guanylylimidodiphosphate (Gpp(NH)p) and forskolin were unaffected.

24 thiol (GTPgammaS) or the 3' ribose-hydroxyl (GppNHp) bond to relieve the quenching of BODIPY fluoresc

25 uanosine-5'-(beta, gamma)-imidotriphosphate (GppNHp).

26 guanosine 5'-(beta,gamma-iminotriphosphate) (GppNHp) and guanosine 5'-(3-O-thiotriphosphate) (GTPgamm

27 led adenylyl cyclase system to isoprenaline, Gpp(NH)p and forskolin was studied by measuring cAMP pro

28 adenylyl cyclase responses to isoproterenol, Gpp(NH)p, and forskolin.

29 A loss of affinity was observed for KRAS-GppNHp, due in part to rearrangements in switch-II, wher

30 tant Ras isoforms (K(D) = 21 nM for KRasG12V-GppNHp) and is highly cell-permeable and metabolically s

31 MANT-GTP gamma S and MANT-GppNHp competitively inhibited forskolin/Mn(2+)-stimulat

32 The K(i) value for MANT-GppNHp at insect cell AC was 155 nm.

33 nhanced the potency of MANT-GTP gamma S/MANT-GppNHp at inhibiting AC by approximately 4-8-fold.

34 Collectively, MANT-GTP gamma S/MANT-GppNHp bind to G alpha(s)- and G alpha(i)-proteins with

35 Instead, MANT-GTP gamma S/MANT-GppNHp constitute a novel class of potent competitive AC

36 MANT-GTP gamma S/MANT-GppNHp had lower affinities for G alpha(s) and G alpha(i

37 MANT-GTP gamma S/MANT-GppNHp non-competitively inhibited GTP gamma S/GppNHp-,

38 AC inhibition by MANT-GTP gamma S/MANT-GppNHp was not due to G alpha(s) inhibition because it w

39 sine 5'-[beta,gamma-imido]triphosphate (MANT-GppNHp) on G alpha(s)- and G alpha(i)-protein-mediated s

40 cess in human H-Ras complexed with GTP mimic GppNHp is global, encompassing most of the GTPase cataly

41 [3H]glyburide (in the presence or absence of Gpp(NH)p which blocks binding to low-affinity sites) to

42 [3H]glyburide in the presence of absence of Gpp(NH)p.

43 [3H]glyburide in the presence or absence of Gpp(NH)p.

44 Addition of Gpp(NH)p also inhibited in vitro (18)F-F13714 binding, c

45 Increasing concentrations of Gpp(NH)p, but not ATP, decreased the specific binding of

46 Comparison of the structures of GppNHp- and GDP-bound RalA suggests a nucleotide-depende

47 ivating protein for Ypt7p:GTP), GTPgammaS or GppNHp (non-hydrolyzable nucleotides), and mutant forms

48 The crystal structure of KRAS Q61R-GppNHp reported here revealed that access to the switch-

49 inhibitors had reduced affinity to KRAS Q61R-GppNHp, but not to WT and other mutants.

50 E) or dithiothreitol (DTT) soaked into H-Ras-GppNHp crystals in the presence of a moderate amount of

51 lizes the conformation observed in the H-Ras-GppNHp/NOR1A complex, and PEG, DTE, and DTT stabilize th

52 r G alpha(s) and G alpha(i) than GTP gamma S/GppNHp as assessed by inhibition of GTP hydrolysis of re

53 Mn(2+) blocked AC inhibition by GTP gamma S/GppNHp in S49 cyc(-) membranes but enhanced the potency

54 pNHp non-competitively inhibited GTP gamma S/GppNHp-, AlF(4)(-)-, beta(2)-adrenoceptor plus GTP-, cho

55 In Sec4-GppNHp, structural features common to active Rab protein

56 Interestingly, we find that GppNHp-bound RhoC only shows differences in its switch I

57 Our results show that GppNHp-bound proteins exhibit significant state 1 popula

58 h peptidomimetics were able to attenuate the Gpp(NH)p-induced shift to the low-affinity state to a gr

59 tsY association previously observed with the GppNHp-bound form.

60 as [125]S(-)5-OH-PIPAT, was not sensitive to Gpp(NH)p.

61 hown here and in other studies is related to GppNHp and is most likely nonexistent in cells.

62 uanosine 5'-(beta, gamma-imido)triphosphate (Gpp(NH)p), inhibits binding of 125I-glucagon to the wild

63 guanosine 5'-(beta,gamma-imido)triphosphate (GppNHp) shifted the agonist-bound receptor into a G prot

64 guanosine-5'-(betagamma-imino)triphosphate (GppNHp) has been determined at a resolution of 1.5 A.

65 These effects were observed when Gpp(NH)p was either absent or present, and they were ana

66 es observed in the respective controls where Gpp(NH)p was absent.

67 karyopherin-beta2-Ran x GppNHp complex where GppNHp is a non-hydrolysable GTP analogue.

68 Even with Gpp(NH)p present, 3 and 4 were able to return the RH/RL

69 0 A structure of the karyopherin-beta2-Ran x GppNHp complex where GppNHp is a non-hydrolysable GTP an

70 Ran x GppNHp in the complex shows extensive structural rearran