ライフサイエンスコーパス: HDX-MS

1 HDX MS revealed conformational changes at the resolution

2 HDX-MS also revealed nucleotide binding induces global c

3 HDX-MS also shows a dramatic increase in the protein-lip

4 HDX-MS analysis shows that SufE binds near the SufS acti

5 HDX-MS and MD of holo-PhuS indicate an overall reduction

6 HDX-MS experiments demonstrate that RAMP2 enhances local

7 HDX-MS identifies a potential Galpha interaction site in

8 HDX-MS identifies an N-terminal amphipathic helix essent

9 HDX-MS of fibrillating hCT (pH 7.4; 25 degrees C) sugges

10 HDX-MS on the intact protein reported exchange in the na

11 HDX-MS relies on successful proteolytic digestion of tar

12 HDX-MS revealed that the apo-PhuS C-terminal alpha6/alph

13 HDX-MS reveals regions of the hMGL that become substanti

14 HDX-MS reveals that while the dominant structural change

15 HDX-MS shows that the conformational dynamics of the two

16 HDX-MS shows that the four Cas7 RNA recognition motif (R

17 HDX-MS shows that Venetoclax more strongly impacts the p

18 HDX-MS thus holds exceptional promise as an enabling ana

19 HDX-MS was also applied to recombinant FIXa variants tha

20 HDX-MS's rapid exchange kinetics of several peptides dem

21 HDX-MS-guided, site-directed ElpQ mutants were generated

22 these enzymes, Nepenthesin II (NepII), in a HDX-MS workflow.

23 m exchange with mass spectrometric analysis (HDX-MS) coupled with proteolytic digestion was used to i

24 However, analyzing HDX-MS data presents a significant challenge due to the

25 a given peptide's isotope distribution, and HDX MS data analysis methods were developed accordingly.

26 mics of hMGL by combining kinetics, NMR, and HDX-MS data with metadynamics simulations.

27 sults also highlight the utility of applying HDX-MS to ADCs to gain a molecular level insight into th

28 ycan level opens the possibility of applying HDX-MS to monitor glycan interactions and dynamics.

29 Here, we describe an automated HDX-MS platform that operates with a parallel, two-trap,

30 escribe the development of a fully automated HDX-MS apparatus to resolve amide exchange on the millis

31 Although the proteolysis-based HDX-MS approach is most commonly used, the "top-down" ap

32 f these compounds as IERs for solution based HDX-MS could considerably extend the utility of the tech

33 This technique will benefit HDX MS measurements for any protein that contains one or

34 rmational heterogeneity was also assessed by HDX MS isotopic distribution.

35 r the FcRn binding interface as indicated by HDX MS and structural modeling; however, HC Met257 oxida

36 nge of proteins amenable to interrogation by HDX MS continues to expand at an accelerating pace, ther

37 the largest protein assembly yet accessed by HDX-MS, with sequence resolution of segments of as few a

38 nt high-throughput screening applications by HDX-MS.

39 uctural dynamics of the receptor assessed by HDX-MS correlate with activity in biochemical and cell-b

40 ity upon antibody binding were identified by HDX-MS, and five different peptides over the same three

41 y map densities with constraints provided by HDX-MS and crosslinking, allowing us to obtain valuable

42 demonstrate that the information provided by HDX-MS experiments and by the model of exchange are suff

43 In such cases, HDX-MS may provide an incomplete view of protein dynamic

44 We combine HDX-MS with mutagenesis and MD simulations to dissect th

45 In contrast, HDX-MS analysis of p38beta did not detect significant te

46 d with a conventional temperature-controlled HDX-MS setup to achieve fast and online removal of unwan

47 In a conventional HDX-MS workflow, disulfide bonds are reduced chemically

48 X-ray crystallography, HDX-MS and SPR analysis confirmed that the CDR regions o

49 olves this problem by dissecting T-dependent HDX-MS profiles into contributions from k(ch)(T), as wel

50 Differential HDX-MS allows comparison of protein states, such as in t

51 Differential HDX-MS of isotopically labeled RT in the presence of the

52 -retest repeatability study for differential HDX-MS experiments implemented at each of two laboratori

53 and visualization of data from differential HDX-MS.

54 Successful applications of differential HDX-MS include the characterization of protein-ligand bi

55 A single differential HDX-MS data set (protein +/- ligand) is often comprised

56 16 muM were characterized using differential HDX-MS (DeltaHDX-MS).

57 kbone protection pattern generated by direct HDX-MS/MS is in excellent agreement with the known cryst

58 ation of the new methodology to the top-down HDX MS characterization of a small (99 residue long) dis

59 protection pattern deduced from the top-down HDX MS measurements carried out under native conditions

60 roteins amenable to the analysis by top-down HDX MS still remains limited, with the protein size and

61 Resolving isotopic fine structure during HDX MS therefore permits direct monitoring of HDX, which

62 ane proteins to proteolytic digestion during HDX-MS is highly protein-specific.

63 electrochemical reduction efficiency during HDX-MS analysis of two particularly challenging disulfid

64 successful electrochemical reduction during HDX-MS analysis of both a small exceptional tightly disu

65 se HalM2 as a model system, we have employed HDX-MS to demonstrate that HalM2 is indeed a highly stru

66 leverage locally bound variables to enhance HDX-MS data modeling.

67 Furthermore, this work shows that in-ESI HDX-MS can be used to monitor the dynamics of solvated m

68 Here, we utilize in-ESI HDX-MS to characterize nine isomeric disaccharides with

69 Hydrogen-deuterium exchange (HDX-MS) mapped onto a full structural model of the ligas

70 icles can be easily incorporated in existing HDX MS workflows to provide more peptide coverage in exp

71 ting for the shallowness of the experimental HDX-MS profiles at low T.

72 d glycoproteins however pose a challenge for HDX-MS.

73 ase pepsin has been the enzyme of choice for HDX-MS studies, recently, it was shown that aspartic pro

74 clude that An-PEP is an archetype enzyme for HDX-MS, highly complementary to pepsin, and especially p

75 expanded the limits of what is feasible for HDX-MS investigations.

76 midazolium carbon acids as superior IERs for HDX-MS.

77 eptidase from Aspergillus niger (An-PEP) for HDX-MS.

78 ) mADA (Ea = 5.0 kcal/mol) were selected for HDX-MS experiments.

79 Finding a microscopic kinetic solution for HDX-MS data provides a window into local protein stabili

80 ndom and gross errors, automated systems for HDX-MS analysis have become routine in many laboratories

81 show that under conditions commonly used for HDX-MS, acetamido groups within glycan chains retain a s

82 ted, however, the data analysis workflow for HDX-MS data with resolved isotopic fine structure is dis

83 ation, and visualization of such events from HDX-MS data sets is challenging as these data sets consi

84 ctions with the conformational readouts from HDX-MS, we have uncovered key lipid-protein interactions

85 Furthermore, HDX-MS revealed that a MinE mutant (D45A/V49A), previous

86 ortant focus will be on a description of how HDX-MS can be used as a powerful tool to optimize the de

87 However, HDX-MS analysis in such systems suffers from extreme spe

88 However, HDX-MS is currently limited by the significant cost of t

89 However, HDX-MS is still an emergent tool for quality control of

90 In this article, an improved HDX MS platform with fully automated data processing is

91 lenges in using electrochemical reduction in HDX-MS analyses and provide possible conditions to atten

92 erium levels at single residue resolution in HDX-MS experiments.

93 that could improve the spatial resolution in HDX-MS studies.

94 enhance the search for a minimum solution in HDX-MS optimization, the ability of selected constrained

95 emical reduction as a substitute for TCEP in HDX-MS analyses.

96 s often comprised of more than 40 individual HDX-MS experiments.

97 s the absence of strategies for interpreting HDX-MS data in the context of T-dependent protein dynami

98 e, we show that the chip is capable of local HDX-MS analysis of hemoglobin with good sensitivity, seq

99 rofluidic chip (HDXchip) for global or local HDX-MS analysis that can be cooled to subzero temperatur

100 gh the application of automated (and manual) HDX-MS has become common, there are only a handful of st

101 g a combination of cryo electron microscopy, HDX-MS, and biochemical assays, we have identified novel

102 trate a new approach correlating millisecond HDX-MS data with aggregation kinetics to determine the l

103 Raman (RR), hydrogen-deuterium exchange MS (HDX-MS) methods, and molecular dynamics (MD).

104 pplied hydrogen-deuterium (H/D) exchange MS (HDX-MS) of full-length Escherichia coli DXPS to provide

105 Hydrogen-deuterium exchange MS (HDX-MS) of p38alpha performed at 33, 37, and 39.5 degree

106 plication of hydrogen-deuterium exchange MS (HDX-MS) with other structural biology techniques to prob

107 g (CIU), and hydrogen-deuterium exchange MS (HDX-MS).

108 This hydrogen deuterium exchange-MS (HDX-MS) study of functional complexes of CF, CheA, and C

109 ydrogen-deuterium exchange kinetics with MS (HDX-MS) to interrogate the high-order structure of prote

110 hydrogen-deuterium exchange coupled with MS (HDX-MS), we probed the RcnR structure in the presence of

111 Here, we present a novel HDX-MS workflow for analysis of the conformational dynam

112 Da glycoprotein transferrin in the course of HDX MS experiments is carried out using electron capture

113 ploited the aforementioned unique feature of HDX MS in combination with the ability of MS to isolate

114 h states previously remained out of reach of HDX MS.

115 verall, we demonstrate the unique ability of HDX-MS to distinguish between the conformational dynamic

116 ages currently available for the analysis of HDX-MS data do not enable the peptide- and ETD-levels to

117 Currently, analysis of HDX-MS data remains a laborious procedure, mainly due to

118 need for breakthroughs in the application of HDX-MS analysis to protein-ligand interactions in highly

119 lished describing a cross-site comparison of HDX-MS experiments.

120 Comparison of HDX-MS profiles between unbound ElpQ and the ElpQ/C1s co

121 Comparison of HDX-MS results for apo GroEL and GroEL-ATPgammaS enables

122 tate objective structural interpretations of HDX-MS data and to inform experimental approaches and fu

123 s C to 55 degrees C for the interrogation of HDX-MS.

124 The successful outcome of HDX-MS analyses depends on the sample preparation condit

125 al assays and kinetic analysis of a panel of HDX-MS guided variant enzymes.

126 systematic evaluation of the performance of HDX-MS experiments, and no reports have been published d

127 d in multiple locations, an understanding of HDX-MS reproducibility is critical.

128 Here, we report on the use of HDX-MS to investigate conformational differences between

129 Despite the widespread use of HDX-MS, it remains unclear whether this technique provid

130 These results highlight the utility of HDX-MS for interrogating the higher-order structure of A

131 Based on HDX-MS studies showing that different cargo rely on diff

132 ing electrochemical reduction into an online HDX-MS workflow.

133 ion or immobilized on-column in an optimized HDX-MS-compatible workflow.

134 ESI interface, allowing for online gas-phase HDX-MS analysis of peptides and proteins separated on a

135 rinopeptide B, confirmed that this gas-phase HDX-MS approach allows for labeling of sites (heteroatom

136 Furthermore, online gas-phase HDX-MS could be performed in tandem with ion mobility se

137 Results from gas-phase HDX-MS of peptides using the aqueous ND3/D2O as HDX reag

138 pectrometer immediately after ESI (gas-phase HDX-MS) and show utility for studying the primary and hi

139 us) not accessed by classical solution-phase HDX-MS.

140 Previous HDX MS methods did not resolve these isotopomers, requir

141 ly unattended analysis of the entire protein HDX MS data set starting from ion detection and peptide

142 e exchangeability of backbone amide protons, HDX-MS can reveal information about higher-order structu

143 ture long gradient UPLC-HDX-MS platform (PTD-HDX-MS) to facilitate high-throughput analysis of protei

144 ifferent hCT residues as indicated by pulsed HDX-MS.

145 ion dissociation is verified using redundant HDX-MS data generated by FTICR-MS.

146 ructural ensembles that faithfully reproduce HDX-MS measurements.

147 exchange-mass spectrometry experiments (SEC-HDX-MS).

148 rogen/deuterium exchange mass spectrometric (HDX-MS) investigation of an antibody-drug conjugate (ADC

149 drogen-deuterium exchange mass spectrometry (HDX MS) experiments.

150 drogen-deuterium exchange mass spectrometry (HDX MS) has become an important technique for the analys

151 drogen/deuterium exchange mass spectrometry (HDX MS) was used in two case studies to evaluate the imp

152 drogen-deuterium exchange mass spectrometry (HDX MS) was used to interrogate the conformational impac

153 drogen/deuterium exchange mass spectrometry (HDX MS), native mass spectrometry, and negative-staining

154 ium exchange coupled with mass spectrometry (HDX MS).

155 drogen Deuterium eXchange-Mass Spectrometry (HDX-MS) analysis also showed that these VHHs mainly targ

156 drogen-deuterium exchange mass spectrometry (HDX-MS) analysis and designing homology-based intragenic

157 drogen-deuterium exchange mass spectrometry (HDX-MS) and chemical crosslinking.

158 drogen deuterium exchange-mass spectrometry (HDX-MS) and differential scanning fluorimetry (DSF) expe

159 drogen/deuterium exchange mass spectrometry (HDX-MS) and molecular dynamics studies revealed that Ca(

160 drogen-deuterium exchange mass spectrometry (HDX-MS) and NMR relaxation dispersion measurements.

161 drogen-deuterium exchange mass spectrometry (HDX-MS) and site-directed mutagenesis using full-length

162 drogen-deuterium exchange mass spectrometry (HDX-MS) and SPR interaction analysis on a library of lys

163 drogen/deuterium-exchange mass spectrometry (HDX-MS) approach capable of precisely locating dynamic s

164 drogen/deuterium exchange mass spectrometry (HDX-MS) approaches have been used to inform on the globa

165 gen deuterium exchange by mass spectrometry (HDX-MS) as a function of temperature in a thermophilic d

166 drogen/deuterium exchange mass spectrometry (HDX-MS) by comparing the deuterium uptake in the bound a

167 drogen-deuterium exchange mass spectrometry (HDX-MS) can be used to map epitopes recognized by pAb sa

168 drogen/deuterium exchange-mass spectrometry (HDX-MS) enables the analysis of protein dynamics by moni

169 ium exchange monitored by mass spectrometry (HDX-MS) enables the study of protein dynamics by measuri

170 drogen-deuterium exchange mass spectrometry (HDX-MS) experiments confirm that activation occurs throu

171 drogen/deuterium-exchange mass spectrometry (HDX-MS) experiments on protein structures can be perform

172 ium exchange coupled with mass spectrometry (HDX-MS) experiments reveal conformational changes in the

173 drogen-deuterium exchange mass spectrometry (HDX-MS) experiments reveal the dynamics of apo Cas7-11.

174 drogen-deuterium exchange mass spectrometry (HDX-MS) experiments were performed as a function of time

175 drogen-deuterium exchange mass spectrometry (HDX-MS) followed by electron-transfer dissociation (ETD)

176 ium exchange monitored by mass spectrometry (HDX-MS) has become a routine approach for sensitive anal

177 erium exchange coupled to mass spectrometry (HDX-MS) has been applied to a mesophilic (E. coli) dihyd

178 erium exchange coupled to mass spectrometry (HDX-MS) has been previously employed to identify spatial

179 drogen/deuterium exchange mass spectrometry (HDX-MS) has emerged as a powerful analytical technique w

180 drogen deuterium exchange-mass spectrometry (HDX-MS) has emerged as a powerful technique for interrog

181 drogen/deuterium exchange-mass spectrometry (HDX-MS) has emerged as a powerful tool to probe protein

182 erium exchange coupled to mass spectrometry (HDX-MS) has emerged as a technique for studying glycopro

183 drogen/deuterium exchange mass spectrometry (HDX-MS) has emerged as a useful tool to understand the s

184 drogen-deuterium exchange mass spectrometry (HDX-MS) in solution.

185 drogen/deuterium exchange mass spectrometry (HDX-MS) in the protein therapeutic field is undisputed;

186 drogen-deuterium exchange mass spectrometry (HDX-MS) indicates that the alpha1 helix of the ECD is EC

187 drogen-deuterium exchange mass spectrometry (HDX-MS) indicates that the domain contains limited hydro

188 drogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increa

189 drogen-Deuterium Exchange Mass Spectrometry (HDX-MS) is a powerful protein characterization technique

190 drogen/deuterium exchange mass spectrometry (HDX-MS) is a powerful technique for analyzing the confor

191 ium exchange coupled with mass spectrometry (HDX-MS) is a powerful technique for the characterization

192 drogen/deuterium exchange mass spectrometry (HDX-MS) is a powerful technique to interrogate protein s

193 drogen-deuterium exchange mass spectrometry (HDX-MS) is a powerful technique to monitor protein intri

194 drogen/deuterium-exchange mass spectrometry (HDX-MS) is a powerful tool for analyzing the conformatio

195 drogen-deuterium exchange mass spectrometry (HDX-MS) is a powerful tool for protein structure analysi

196 drogen deuterium exchange mass spectrometry (HDX-MS) is a rapidly growing technique for protein chara

197 change (HDX) coupled with Mass Spectrometry (HDX-MS) is a sensitive and robust method to probe protei

198 um exchange combined with mass spectrometry (HDX-MS) is a widely applied biophysical technique that p

199 drogen-Deuterium Exchange Mass Spectrometry (HDX-MS) is a widespread tool for studying protein struct

200 drogen/deuterium exchange mass spectrometry (HDX-MS) is an established technique for the study of pro

201 drogen-deuterium exchange mass spectrometry (HDX-MS) is an established, powerful tool for investigati

202 ium exchange coupled with mass spectrometry (HDX-MS) is an information-rich biophysical method for th

203 drogen deuterium exchange mass spectrometry (HDX-MS) is becoming increasing routine for monitoring ch

204 drogen-deuterium eXchange mass spectrometry (HDX-MS) is increasingly used in drug development to loca

205 drogen-deuterium exchange mass spectrometry (HDX-MS) is increasingly utilized in the pharmaceutical i

206 drogen/deuterium exchange mass spectrometry (HDX-MS) is now a routinely used technique to inform on p

207 erium eXchange coupled to Mass Spectrometry (HDX-MS) is now common practice in structural biology.

208 ium exchange coupled with mass spectrometry (HDX-MS) is widely used for monoclonal antibody (mAb) epi

209 drogen/deuterium exchange-mass spectrometry (HDX-MS) mapped GCN2-ribosome interactions to domain II o

210 drogen/deuterium exchange mass spectrometry (HDX-MS) mean that the two methods are being more frequen

211 drogen-deuterium exchange/mass spectrometry (HDX-MS) measurements in E. coli outer membranes find tha

212 drogen/deuterium exchange mass spectrometry (HDX-MS) of complexes I and II on membranes elucidated st

213 drogen-deuterium exchange mass spectrometry (HDX-MS) of membrane proteins incorporated into nanodiscs

214 drogen/deuterium-exchange mass spectrometry (HDX-MS) provides detailed insight into the structural dy

215 drogen/deuterium exchange mass spectrometry (HDX-MS) requires effective and rapid reduction of disulf

216 ium exchange monitored by mass spectrometry (HDX-MS) requires reduction of the disulfide bonds under

217 ange studies coupled with mass spectrometry (HDX-MS) show that upon interaction with lipid, the centr

218 drogen-deuterium exchange mass spectrometry (HDX-MS) studies revealed that ANGPTL3/8 and ANGPTL3 use

219 drogen-deuterium exchange mass spectrometry (HDX-MS) studies show that local HalM2 structural dynamic

220 drogen/deuterium exchange mass spectrometry (HDX-MS) to access E. coli chaperonin GroEL conformation.

221 drogen/deuterium-exchange mass spectrometry (HDX-MS) to analyze conformational changes in NhaA upon L

222 drogen/deuterium exchange mass spectrometry (HDX-MS) to characterize interactions of low affinity pep

223 drogen/deuterium exchange mass spectrometry (HDX-MS) to characterize SufS-SufE interactions and prote

224 drogen Deuterium Exchange Mass Spectrometry (HDX-MS) to explore the structural dynamics of the turkey

225 drogen deuterium exchange mass spectrometry (HDX-MS) to identify critical residues for RNA interactio

226 drogen-deuterium exchange-mass spectrometry (HDX-MS) to investigate how FIXa responds to assembly wit

227 drogen-deuterium exchange mass spectrometry (HDX-MS) to investigate membrane protein conformational d

228 erium exchange coupled to mass spectrometry (HDX-MS) to investigate the effects on HBV capsids of bin

229 drogen-deuterium exchange mass spectrometry (HDX-MS) to map the impact of the new disulfide bond on t

230 drogen-deuterium exchange mass spectrometry (HDX-MS) to obtain a comprehensive view of transporter dy

231 n/deuterium exchange with mass spectrometry (HDX-MS) to probe epitope targeting in the context of the

232 drogen-deuterium exchange mass spectrometry (HDX-MS) to reveal conformational changes accompanying me

233 drogen-deuterium exchange mass spectrometry (HDX-MS) to reveal striking nucleotide-dependent conforma

234 drogen-deuterium exchange mass spectrometry (HDX-MS) to study the biophysical properties of a RiPP bi

235 drogen-deuterium exchange-mass spectrometry (HDX-MS) was employed to analyze the backbone dynamics pr

236 erium exchange coupled to mass spectrometry (HDX-MS) was used to map the membrane and NDP52 binding s

237 erium exchange coupled to mass spectrometry (HDX-MS) we rule out a model in which the two forms are i

238 drogen-Deuterium Exchange-Mass Spectrometry (HDX-MS) we show that Ric-8A disrupts the secondary struc

239 drogen/deuterium exchange mass spectrometry (HDX-MS) with a recently developed Fenton chemistry-based

240 ium exchange coupled with mass spectrometry (HDX-MS), and facilitating interpretation of the data wit

241 drogen-deuterium exchange mass spectrometry (HDX-MS), chemical cross-linking mass spectrometry (XL-MS

242 drogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA

243 drogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA

244 drogen/deuterium exchange-mass spectrometry (HDX-MS), especially when applied to larger proteins (ove

245 drogen/deuterium exchange mass spectrometry (HDX-MS), fast photochemical oxidation of proteins (FPOP)

246 drogen deuterium exchange mass spectrometry (HDX-MS), small angle X-ray scattering (SAXS) and molecul

247 drogen/deuterium exchange mass spectrometry (HDX-MS).

248 drogen/deuterium exchange mass spectrometry (HDX-MS).

249 drogen/deuterium exchange mass spectrometry (HDX-MS).

250 drogen/deuterium-exchange mass spectrometry (HDX-MS).

251 drogen-deuterium exchange mass spectrometry (HDX-MS).

252 d in the adoption of isotopic fine structure HDX-MS by providing an intuitive workflow and interface

253 Isotopic fine structure HDX-MS offers the potential to increase sequence coverag

254 In the second case study, HDX MS demonstrated that oxidation of the two complement

255 Until this study, HDX MS experiments employed mass spectral resolving powe

256 anges driven by both cofactor and substrate, HDX-MS highlighted several surface loops that have been

257 As a bottom-up technique, HDX-MS provides information at peptide-level resolution,

258 Together, these results suggested that HDX MS is a powerful tool for evaluating the impact of i

259 his work is not only a proof-of-concept that HDX-MS can be used to get structural insights on large m

260 We conclude that HDX-MS has the potential to visualize the functional imp

261 Our results demonstrate that HDX-MS can validate and map weak peptide-protein interac

262 We show that HDX-MS can identify regions in the two epigenetic regula

263 Together, these results suggest that HDX-MS of His C2-hydrogens is a promising new method for

264 The HDX MS profile of receptor bound to carazolol is consist

265 nalysis of peptide isotopic envelopes in the HDX MS raw data and HDsite for residue-level resolution.

266 The utility of the HDX MS platform is demonstrated by exploring the conform

267 The HDX-MS workflow was optimized to accurately detect low-a

268 As the HDX-MS community continues to grow, adoption of best pra

269 Consequently, the HDX-MS data quality is largely determined by the number

270 e ground up to fulfill a greater role in the HDX-MS analysis pipeline.

271 ction of protein dynamics information in the HDX-MS data is achieved.

272 ained high-sequence coverage (88-99%) in the HDX-MS experiments for full-length BM3 and its component

273 and different quench buffer additives in the HDX-MS toolbox and the need to carefully screen a range

274 atographic phospholipid trap column into the HDX-MS apparatus to enable online sample delipidation pr

275 mplementing an electrochemical cell into the HDX-MS workflow.

276 ns can be challenging, severely limiting the HDX-MS experiment.

277 for increasing the spatial resolution of the HDX-MS experiment, capable of yielding high fragmentatio

278 his work highlights the broad utility of the HDX-MS platform for revealing important biophysical prop

279 for the first time the applicability of the HDX-MS technique to monitor structural changes due to me

280 handling and increase the versatility of the HDX-MS technique.

281 tion conditions to successfully optimize the HDX-MS analysis of integral membrane proteins.

282 In summary, the HDX-MS workflow with integrated PNGase A deglycosylation

283 (GAP)-MAF1b interface further supporting the HDX-MS data.

284 ing the intact protein on beads prior to the HDX-MS experiment.

285 Using this HDX-MS approach, we have identified novel precursor pept

286 A level of complexity that is inherent to HDX MS remained unaddressed, namely, various combination

287 uld be too massive to be readily amenable to HDX-MS.

288 In traditional HDX-MS, proteins are incubated in D(2)O as a function of

289 Under typical HDX-MS conditions and using small amounts of enzyme, An-

290 ducibility of continuous-labeling, bottom-up HDX-MS measurements, the present interlaboratory compari

291 r our subzero-temperature long gradient UPLC-HDX-MS platform (PTD-HDX-MS) to facilitate high-throughp

292 iscuss specific examples of how we have used HDX-MS to study phosphoinositide kinases and the protein

293 ns, which can be tested experimentally using HDX-MS.

294 Here, using HDX-MS, we identified changes in spike dynamics that we

295 anel of ATP-competitive ERK inhibitors using HDX-MS and NMR and identify 14 new molecules with proper

296 pport for this hypothesis was obtained using HDX-MS which revealed protection to exchange only within

297 In this study, we utilized HDX-MS combined with structural modeling to examine the

298 Furthermore, whereas HDX-MS does not indicate HOS changes, FPOP-MS footprinti

299 in or NepII under conditions compatible with HDX-MS was performed to examine protease cleavage specif

300 reporters (IERs) that readily integrate with HDX-MS to provide a direct measure of exchange condition