ライフサイエンスコーパス: HGPRT

1 HGPRT-deficient fibroblast cells also exhibited the grea

2 After 1 h in D2O, HGPRT alone exchanges 160, HGPRT-GMP-Mg(2+) exchanges 154, the equilibrium complex

3 s study, we examined the ability of N2aTG, a HGPRT-deficient neuroblastoma and its HGPRT-positive cou

4 rse reactions were largely unchanged for all HGPRT constructs, except for a 4-5-fold decrease in the

5 ographic studies of the HGPRT-GMP-Mg(2+) and HGPRT-ImmGP-MgPPi complexes.

6 ntermediate between the sizes of HGPRT-I and HGPRT-II.

7 ct molecular mass for HGPRT-I, HGPRT-II, and HGPRT-I.HGPRT-II and suggests that the predominant heter

8 phage to which the parasites have access are HGPRT or XPRT substrates.

9 Since conversion of T-705 by HGPRT appears to be inefficient, T-705-RMP prodrugs may

10 phosphoribosylation of purine nucleobases by HGPRT.

11 he predicted amino acid sequences of certain HGPRT proteins from parasites of the Trypanosomatidae fa

12 n both isozymes are co-expressed in E. coli, HGPRT-I.HGPRT-II heterotetramers form.

13 e, and the transition-state analogue complex HGPRT-ImmGP-MgPPi.

14 After 1 h in D2O, HGPRT alone exchanges 160, HGPRT-GMP-Mg(2+) exchanges 15

15 These data demonstrate that the L. donovani HGPRT is compartmentalized exclusively within the glycos

16 e employed to establish that the L. donovani HGPRT is localized exclusively to the glycosome.

17 and genetically for the Leishmania donovani HGPRT employing a combination of protein modifying reage

18 These data establish genetically that either HGPRT or XPRT is absolutely essential for purine acquisi

19 direct support to the hypothesis that either HGPRT or XPRT is crucial for purine salvage by the paras

20 to the expression of salvage pathway enzyme HGPRT.

21 eobase transport, the purine salvage enzymes HGPRT, XPRT and APRT were also upregulated, suggesting t

22 targeting signal had been deleted expressed HGPRT throughout the parasite, including subcellular org

23 f the encoded proteins, i.e. allopurinol for HGPRT and 4-aminopyrazolopyrimidine for APRT.

24 veals species of distinct molecular mass for HGPRT-I, HGPRT-II, and HGPRT-I.HGPRT-II and suggests tha

25 se results to reaction coordinate motion for HGPRT suggests that bond formation between C1' of the nu

26 in the following article shows how T. gondii HGPRT is able to recognize guanine, hypoxanthine, and xa

27 transition state structure is of a T. gondii HGPRT mutant (Asp150Ala or D150A).

28 espectively) compared to wild-type T. gondii HGPRT.

29 GMP-Mg(2+) complex, the HGPRT-IMP-MgPPi <==> HGPRT-Hx-MgPRPP equilibrating mixture, and the transitio

30 This structure shows how HGPRT uses two Mg(2+) ions to orient and activate the py

31 Human HGPRT has 207 amide H/D exchange sites.

32 17) exhibited a Ki value of 30 nM for human HGPRT and 70 nM for Pf HGXPRT.

33 exhibited Ki values of 6 and 70 nM for human HGPRT and Pf HGXPRT, respectively.

34 705 and T-1105 are poor substrates for human HGPRT having Km(app) values of 6.4 and 4.1 mM, respectiv

35 nge of K(i) values and selectivity for human HGPRT, PfHGXPRT, and PvHGPRT.

36 p180 at the corresponding locations in human HGPRT and Giardia lamblia GPRT, respectively, may explai

37 on of an LND-associated mutant form of human HGPRT (I42T), but not the wild-type enzyme, resulted in

38 namic and conformational properties of human HGPRT alone, the HGPRT-GMP-Mg(2+) complex, the HGPRT-IMP

39 compounds into the known structures of human HGPRT in complex with ANP-based inhibitors suggests reas

40 roM and a 30-fold selectivity over the human HGPRT.

41 d selectivity for PfHGXPRT compared to human HGPRT.

42 six of these compounds in complex with human HGPRT have been determined.

43 xy)ethylguanine (PEEG) in complex with human HGPRT have led to the design of new ANPs.

44 structure of T-705-RMP in complex with human HGPRT showed how this compound binds in the active site.

45 cture of this compound in complex with human HGPRT shows that it fills or partially fills three criti

46 structure of this ANbP in complex with human HGPRT was determined at 2.0 A resolution and shows that

47 cies of distinct molecular mass for HGPRT-I, HGPRT-II, and HGPRT-I.HGPRT-II and suggests that the pre

48 ular mass for HGPRT-I, HGPRT-II, and HGPRT-I.HGPRT-II and suggests that the predominant heterotetrame

49 sozymes are co-expressed in E. coli, HGPRT-I.HGPRT-II heterotetramers form.

50 ovani, null mutants genetically deficient in HGPRT and/or APRT were generated by targeted gene replac

51 An essential role for this Ser-Tyr dyad in HGPRT catalysis has now been verified biochemically and

52 nalog lacking the 6-fluoro atom) was lost in HGPRT-deficient Madin-Darby canine kidney cells.

53 vated c-MYC expression leads to an increased HGPRT mutation rate of Rat1 cells and an increase in the

54 aTG, a HGPRT-deficient neuroblastoma and its HGPRT-positive counterpart to proliferate and differenti

55 rising ability of mutant L. donovani lacking HGPRT, APRT, and/or AK to incorporate and grow in hypoxa

56 in T. foetus HGXPRT and Arg187 in S. mansoni HGPRT will be attractive targets for future studies.

57 Mutant HGPRTs, S95A, S95E, Y96F, and Y96V, exhibited dramatic r

58 nts containing amplified copies of a mutated HGPRT construct in which the Ser-Lys-Val COOH-terminal t

59 No HGPRT protein was detected in delta hgprt null mutants i

60 nd phylogenetic analysis, we confirm that no HGPRT activity is expressed in Drosophila melanogaster,

61 The specific activity of HGPRT-I is five times that of HGPRT-II.

62 ssion plasmid contained augmented amounts of HGPRT, all of which was localized to the glycosome.

63 to the transition state analogue complex of HGPRT.ImmHP.MgPP(i) to determine the ionic states of the

64 Incubation of HGPRT with either tetranitromethane or diethyl pyrocarbo

65 ine definitively the intracellular milieu of HGPRT in these pathogens, polyclonal antiserum to the pu

66 ts size is intermediate between the sizes of HGPRT-I and HGPRT-II.

67 ic activity of HGPRT-I is five times that of HGPRT-II.

68 The effect on HGPRT mutation rate is small (2-3-fold), but is consiste

69 e predominant heterotetramer consists of one HGPRT-I subunit and three HGPRT-II subunits.

70 he residue corresponding to Asp206, the only HGPRT amino acid that directly contacts the Mg(2+) ions,

71 (HGPRT) and significant homology with other HGPRT family members.

72 oxanthine-guanine phosphoribosyltransferase (HGPRT) and Plasmodium falciparum (Pf) hypoxanthine-guani

73 oxanthine-guanine phosphoribosyltransferase (HGPRT) and significant homology with other HGPRT family

74 oxanthine-guanine phosphoribosyltransferase (HGPRT) and xanthine phosphoribosyltransferase (XPRT).

75 oxanthine-guanine phosphoribosyltransferase (HGPRT) are two structurally related enzymes involved in

76 oxanthine guanine phosphoribosyltransferase (HGPRT) converts T-705 into its ribose-5'-monophosphate (

77 oxanthine-guanine phosphoribosyltransferase (HGPRT) have been determined at 1.65 and 1.90 A resolutio

78 oxanthine-guanine phosphoribosyltransferase (HGPRT) is a key enzyme in the purine salvage pathway of

79 oxanthine-guanine phosphoribosyltransferase (HGPRT) is the key enzyme in purine base salvage in human

80 oxanthine-guanine phosphoribosyltransferase (HGPRT) locus.

81 oxanthine-guanine phosphoribosyltransferase (HGPRT) of the apicomplexan protozoan Toxoplasma gondii a

82 oxanthine-guanine phosphoribosyltransferase (HGPRT) proteins have implied that the translocation of a

83 oxanthine-guanine phosphoribosyltransferase (HGPRT), adenine phosphoribosyltransferase (APRT), and ad

84 oxanthine-guanine phosphoribosyltransferase (HGPRT)-xanthosine 5'-monophosphate (XMP)-pyrophosphate-M

85 oxanthine-guanine phosphoribosyltransferase (HGPRT).

86 oxanthine-guanine phosphoribosyltransferase (HGPRT).

87 oxanthine-guanine phosphoribosyltransferase (HGPRT).

88 clonal antiserum to the purified recombinant HGPRT from Leishmania donovani was generated in rabbits,

89 ichia coli indicated that wild type and S95T HGPRTs complemented bacterial phosphoribosyltransferase

90 ine PRT in our possession, only schistosomal HGPRT, the only other enzyme that contains an arginine r

91 Toxoplasma gondii are encoded by the single HGPRT gene as a result of differential splicing.

92 Inherited mutations that suppress HGPRT activity are associated with Lesch-Nyhan disease (

93 he Mg(2+) ions, causes Lesch-Nyhan syndrome (HGPRT(Kinston), D193N).

94 encing of this EcoRI fragment confirmed that HGPRT and XPRT were organized in a head-to-tail arrangem

95 These genetic studies indicate that HGPRT, APRT, and AK, individually or in any combination,

96 ational properties of human HGPRT alone, the HGPRT-GMP-Mg(2+) complex, the HGPRT-IMP-MgPPi <==> HGPRT

97 e in the number of frameshift mutants at the HGPRT locus.

98 PRT alone, the HGPRT-GMP-Mg(2+) complex, the HGPRT-IMP-MgPPi <==> HGPRT-Hx-MgPRPP equilibrating mixtu

99 e that is conserved among all members of the HGPRT family is essential for phosphoribosylation of pur

100 rt null mutants in which both alleles of the HGPRT locus had been replaced by a drug-resistance casse

101 A comprehensive description of the HGPRT reaction mechanism is also proposed.

102 defined from crystallographic studies of the HGPRT-GMP-Mg(2+) and HGPRT-ImmGP-MgPPi complexes.

103 Two structural features important to the HGPRT mechanism, a previously unrecognized active site l

104 ith two subunits in the asymmetric unit; the HGPRT tetramer is completed by the application of 2-fold

105 This HGPRT dependency was confirmed in human embryonic kidney

106 er consists of one HGPRT-I subunit and three HGPRT-II subunits.

107 inding of purines and ribose 5'-phosphate to HGPRT.

108 rotetramer has enzymatic activity similar to HGPRT-II, and gel filtration chromatography demonstrates

109 xanthine-guanine phosphoribosyl-transferase (HGPRT) and xanthine phosphoribosyltransferase (XPRT) usi

110 human embryonic kidney 293T cells undergoing HGPRT-specific gene knockdown followed by influenza viru

111 ow as 0.08 and 0.01 muM for Plasmodium vivax HGPRT (PvHGPRT).

112 bilities of 2-3 orders of magnitude, whereas HGPRTs containing conservative substitutions, S95C and S

113 ta hgprt knockout strain in which a wildtype HGPRT was amplified on an expression plasmid contained a

114 as a single copy gene that co-localized with HGPRT within a 4.3-kilobase pair (kb) EcoRI fragment, im

115 emented weakly, and the S95E, Y96F, and Y96V HGPRT did not support bacterial growth.