ライフサイエンスコーパス: HP1

1 HP1 and SUV39H1/2 are repressive factors essential for H

2 HP1 binding to the CPC becomes particularly important wh

3 HP1 binds to the H3K9me3 and recruits the DNA methyltran

4 HP1 can bridge chromatin segments and form liquid drople

5 HP1 is proposed to use oligomerization to compact chroma

6 HP1 is required to recruit the DMM complex to the edges

7 HP1 proteins are central to the assembly and spread of h

8 HP1(Hsalpha)-containing heterochromatin is located near

9 HP1, EZH2, and MeCP2 in turn were associated with additi

10 ng the highly conserved hydrophobic patch 1 (HP1) and that this binding is similar to the binding of

11 umulated abundant heterochromatin protein 1 (HP1) after replicating in S phase 14.

12 s associate with hetereochromatin protein 1 (HP1) alpha- and HP1beta-containing heterochromatic foci.

13 cts directly with heterochromatin protein 1 (HP1) and that this interaction is mediated by an evoluti

14 tion (H3K9me) and heterochromatin protein 1 (HP1) are hallmarks of repression.

15 gated the role of heterochromatin protein 1 (HP1) during the DDR process.

16 The heterochromatin protein 1 (HP1) family members are canonical effectors and propagat

17 Heterochromatin protein 1 (HP1) family proteins are conserved chromatin binding pro

18 The heterochromatin protein 1 (HP1) family proteins HPL-1 and HPL-2 are dispensable for

19 hosphorylation of Heterochromatin Protein 1 (HP1) family proteins regulates heterochromatin dynamics,

20 s are tethered to heterochromatin protein 1 (HP1) for coordinated chromatin movement and histone modi

21 evolving X-linked heterochromatin protein 1 (HP1) gene, HP1D2, plays a key role in the classical Pari

22 Heterochromatin protein 1 (HP1) has been proposed to drive heterochromatin formatio

23 Heterochromatin protein 1 (HP1) has been proposed to protect centromeric sister-chr

24 The heterochromatin protein 1 (HP1) homolog Rhino binds to the H3K9me3 mark through its

25 er gene codes for heterochromatin protein 1 (HP1) in Drosophila.

26 9) and binding of heterochromatin protein 1 (HP1) in the promoter regions of these genes were substan

27 Tethered heterochromatin protein 1 (HP1) induced H3K9me3, DNA methylation, and gene silencin

28 newly identified Heterochromatin Protein 1 (HP1) interaction motif that mediates direct binding betw

29 Heterochromatin protein 1 (HP1) is a central factor in establishing and maintaining

30 ere, we show that heterochromatin protein 1 (HP1) is an essential CPC component required for full Aur

31 Heterochromatin protein 1 (HP1) is well known as a silencing protein found at peric

32 wn of TBX2, EGR1, Heterochromatin Protein 1 (HP1) isoforms and the generic HP1-associated corepressor

33 H3K9me3 marks and heterochromatin protein 1 (HP1) on the reporter locus.

34 stribution of the heterochromatin protein 1 (HP1) ortholog HPL-2 and compared its distribution to oth

35 rase Clr4 and the heterochromatin protein 1 (HP1) ortholog Swi6 are critical for RNAi, whereas RNAi s

36 Heterochromatin protein 1 (HP1) proteins are "gatekeepers" of epigenetic gene silen

37 Heterochromatin protein 1 (HP1) proteins are key factors of eukaryotic heterochroma

38 of the ability of heterochromatin protein 1 (HP1) proteins to spread across large regions of the geno

39 rs and recruiting heterochromatin protein 1 (HP1) proteins.

40 ly interacts with heterochromatin protein 1 (HP1) to promote heterochromatin stability.

41 Binding of heterochromatin protein 1 (HP1) to the histone H3 lysine 9 trimethylation (H3K9me3)

42 nteracts with the Heterochromatin Protein 1 (HP1) variant Rhino (Rhi).

43 w domain (CSD) of heterochromatin protein 1 (HP1) was recently shown to contribute to chromatin bindi

44 Heterochromatin protein 1 (HP1), a highly conserved non-histone chromosomal protein

45 ere, we show that heterochromatin protein 1 (HP1), an H3K9me2 and 3 "reader," interacts with SUV39H1,

46 ), the homolog of heterochromatin protein 1 (HP1), down-regulates the UPR in the intestine.

47 binding proteins heterochromatin protein 1 (HP1), polycomb protein complex 1 (PRC1) and methyl CpG b

48 ly antagonized by heterochromatin protein 1 (HP1), the code reader for histone H3 lysine 9 methylatio

49 romatin spread is heterochromatin protein 1 (HP1), which recognizes H3K9-methylated chromatin, oligom

50 is facilitated by heterochromatin protein 1 (HP1), which serves as an adapter protein.

51 ransferase DIM-5, Heterochromatin Protein 1 (HP1), which specifically binds to the product of DIM-5 (

52 cruiting multiple heterochromatin protein 1 (HP1)-containing complexes that deacetylate histones and

53 ves enrichment of heterochromatin protein 1 (HP1).

54 le association to heterochromatin protein 1 (HP1).

55 logue of metazoan heterochromatin protein 1 (HP1).

56 s binding partner heterochromatin protein 1 (HP1).

57 binding site for heterochromatin protein 1 (HP1).

58 The role of Heterochromatin Protein-1 (HP1) during mitosis has been controversial.

59 We find that (1) HP1 has only a weak capacity to form liquid droplets in

60 Mutants in genes encoding HPL-2/HP1, LIN-13, LIN-61, LET-418/Mi-2, and H3K9me2 histone m

61 how that DIM-2-dependent RIP requires DIM-5, HP1, and other known heterochromatin factors, implying a

62 ther, the present structural analysis of 7SK HP1 highlights an original mechanism of swapping bases,

63 nding site embedded in the 5-hairpin of 7SK (HP1) encompasses a short signature sequence, a GAUC repe

64 e-specific nuclear Argonaute HRDE1/WAGO-9, a HP1 ortholog HPL-2, and two putative histone methyltrans

65 that inhibiting GRPR signaling, or ablating HP1(Hsbeta) expression, increases colon cancer cell inva

66 and 3 "writer," and with TRIM28, an abundant HP1 scaffolding protein, to form complexes with increase

67 Although HP1 links H3K9me3 to DNA methylation, it also serves to

68 acting via the nuclear RNAi machinery and an HP1-like protein, are capable of driving chromatin compa

69 novo centromeres in C. elegans embryos in an HP1-independent manner and suggest that, rather than bei

70 asis for the functional specialization of an HP1 isoform.

71 s nucleate at the core but require DIM-5 and HP1 for spreading.

72 lear factors such as lamin A/C, lamin B, and HP1.

73 the endogenous centromere via DNA breaks and HP1-dependent epigenetic inactivation.

74 The multivalent engagement with DNA and HP1 results in a nucleosome binding circuit in which KDM

75 h as dimethylated histone H3K9 (H3K9me2) and HP1.

76 of ATRX to interact with H3K9me3 histone and HP1.

77 o study the distribution of HipHop, HOAP and HP1 using chromatin immunoprecipitation (ChIP).

78 H3K9me3-modified chromatin through HP1, and HP1 can be recruited to unmodified chromatin by KDM2A.

79 and melanoma, respectively, HP1(Hsalpha) and HP1(Hsbeta) have been shown to modulate the aggressivene

80 st in humans: HP1(Hsalpha), HP1(Hsbeta), and HP1(Hsgamma).

81 ctural studies show that both HP1-INCENP and HP1-Sgo1 interactions require the binding of the HP1 chr

82 terminal repeat region, recruiting KAP1 and HP1, and imposing repressive histone marks.

83 tion of chromatin binding for both KDM2A and HP1 that is modulated by DNA- and H3K9-methylation, and

84 at mediates direct binding between KDM2A and HP1.

85 haracterized by histone H3K9 methylation and HP1 protein binding, silences the underlying DNA and inf

86 contain histone H3 lysine 9 methylation and HP1.

87 Although H3K9 methyltransferases and HP1 are necessary for proper heterochromatin structure,

88 at recognition of methylated nucleosomes and HP1 spread on chromatin are structurally coupled and imp

89 to heterochromatin, suggesting that ORC and HP1 proteins are mutually required for each other to bin

90 as insulators to block the spread of Sir and HP1 mediated silencing while in metazoans most insulator

91 ated the levels of unphosphorylated STAT and HP1 [encoded by Su(var)205] in Drosophila and examined t

92 The HP64-f/HP64-r for ureA and HP1/HP2 for 16S rRNA individually had sensitivities and

93 echanism of regulating the expression of any HP1 isoform in any context has not yet been identified.

94 These include key components such as HP1, Trithorax-like (GAGA factor), Su(var)3-9, Brahma, M

95 AP56 colocalizes with the cluster-associated HP1 variant Rhino, that nuage granules containing Vasa l

96 a minimal fold similar to that of bacterial HP1 integrase and defines structural elements conserved

97 tains at its core the complex formed between HP1 proteins and chromatin that is methylated on histone

98 rochromatin by bridging interactions between HP1 and histone deacetylase complexes.

99 viously unrecognized but direct link between HP1 and CPC localization in the centromere and illustrat

100 and illustrate the critical role of borealin-HP1 interaction in orchestrating an accurate cell divisi

101 n the midzone is independent of the borealin-HP1 interaction, demonstrating the spatial requirement o

102 This borealin-HP1 interaction recruits the CPC to the centromere and g

103 emical and structural studies show that both HP1-INCENP and HP1-Sgo1 interactions require the binding

104 lly, Epe1 is recruited to heterochromatin by HP1 silencing factors that are distributed throughout he

105 methylation (H3K9me) and its recognition by HP1 proteins are necessary for pericentromeric heterochr

106 methylation (H3K9Me) and its recognition by HP1 proteins.

107 1gamma), that binds to a conserved canonical HP1-binding motif, PXVXL, in the C-terminal domain of TI

108 Clearly, HP1 function is altered by context, and potentially by p

109 results were simulated by the numerical code HP1 (Hydrus-PhreeqC) with the DLVO theory, extended coll

110 cycle 15, satellites clustered in a compact HP1-positive mass, but replication occurred at decondens

111 We also suggest that compromising HP1 expression could promote tumorigenesis by impairing

112 including an acidic stretch and a consensus HP1-binding motif.

113 Consequently, HP1 has many important roles in chromatin architecture a

114 TBX2 interacts with HP1 through a conserved HP1-binding motif in its N-terminus, which in turn leads

115 where they also interact with the conserved HP1 protein.

116 otif within CCM2 binds the highly conserved "HP1" pocket of the CCM3 focal adhesion targeting (FAT) h

117 ipts and SarA protein than the corresponding HP1 stem and the HP2 stem and loop mutations, leading to

118 ed HDAC4 nuclear export, H3K9 demethylation, HP1 dissociation from the promoter region, and activatio

119 Collectively our results demonstrate HP1-alpha oligomerization is critical to the maintenance

120 Depleting HP1 reduced recruitment of BRCA1 to DSBs and caused defe

121 Depleting HP1-gamma up-regulated proliferation-promoting genes in

122 In contrast, depleting HP1 from cells did not affect the non-homologous end-joi

123 How different HP1 proteins, which are highly conserved, perform differ

124 The Drosophila HP1 homolog Rhino is required for germline piRNA product

125 entiating the interactions of the Drosophila HP1 paralogs.

126 me3 histone mark and its downstream effector HP1.

127 vivo interactions with the chromatin factors HP1 and SMC3 and the cofactors Sin3A and YB-1.

128 echanism is not clarified yet especially for HP1.

129 models have been obtained experimentally for HP1.

130 our results point to multiple functions for HP1 in different cell types to maintain ER homeostasis.

131 ively, these studies reveal a novel role for HP1 as a cofactor in tumor suppression, expand our mecha

132 H enzymes, which provides a docking site for HP1 proteins, therefore mediating heterochromatin compac

133 P gene copy number variation (CNV) generates HP1 and HP2 alleles, while the single-nucleotide polymor

134 tin Protein 1 (HP1) isoforms and the generic HP1-associated corepressor protein KAP1 all resulted in

135 chotomised HP into HP1-containing genotypes (HP1-1 and HP2-1) and HP2-2 to evaluate the HP1 allele.

136 alter the hydrogen-bonding regime in the H3-HP1 complex.

137 themselves provide a binary switch in the H3-HP1 system, but arginine-phosphoserine interactions, whi

138 ector backbone localizing within an H3K9me3, HP1-enriched core.

139 ding of the protein HEXIM to the 5' hairpin (HP1) of 7SK RNA.

140 Species that have HP1 proteins possess multiple paralogs that perform non-

141 laying a structural role in heterochromatin, HP1 proteins can have both an activating as well as repr

142 In addition to its role in heterochromatin, HP1 proteins have been shown to function in transcriptio

143 Unexpectedly, a second hit, HIPP1 (HP1 and insulator partner protein-1) (CG3680), is strong

144 How HP1 proteins assemble on methylated nucleosomal template

145 multidisciplinary approach to determine how HP1(Hsalpha)-nucleosome interactions contribute to the s

146 Yet, how HP1-mediated phase separation relates to chromatin compa

147 The two alleles of HP, HP1 and HP2, differ for 2 extra exons in HP2 that result

148 rent isoforms exist in humans: HP1(Hsalpha), HP1(Hsbeta), and HP1(Hsgamma).

149 demonstrate that the CSDs of all three human HP1 homologs have comparable affinities to the PXXVXL mo

150 ch three different isoforms exist in humans: HP1(Hsalpha), HP1(Hsbeta), and HP1(Hsgamma).

151 Mechanistic studies implicate HP1 family proteins as 'hub proteins,' able to interact

152 ontrast, INCENP or Sgo1 mutants deficient in HP1 binding fail to localize to centromeres in interphas

153 Consistently, a Sgo1 mutant deficient in HP1 binding is functional in centromeric cohesion protec

154 A KDM2A mutant deficient in HP1-binding is inactive in an in vivo overexpression ass

155 ns of HP1(Hsalpha) play an essential role in HP1(Hsalpha)-nucleosome interactions, whereas the hinge

156 umulation of repressive complexes, including HP1, the NuRD complex, H2A.Z and histone methyltransfera

157 ound that a cohort of these agents inhibited HP1-mediated gene silencing and one lead compound potent

158 KLF11-regulated cancer genes, by inhibiting HP1-SUV39H1 recruitment, decreasing H3K9me3, while incre

159 -1, 2-1 or 2-2 and also dichotomised HP into HP1-containing genotypes (HP1-1 and HP2-1) and HP2-2 to

160 heterochromatin foci lack a separated liquid HP1 pool; and (4) heterochromatin compaction can toggle

161 Biologically, impairment of KLF11-mediated HP1-HMT recruitment abolishes tumor suppression, providi

162 Most organisms encode for multiple HP1 isoforms and the molecular mechanisms that underpin

163 Neutralizing HP1 function dysregulated the formation of DERE-involved

164 ently show that the helical hairpin HP2, not HP1, serves as an intracellular gate (in addition to its

165 unctions of HP1, we sought to identify novel HP1-interacting proteins.

166 P distribution was altered in the absence of HP1, the chromodomain protein that binds to H3K9me3.

167 We find from fluctuation analysis of HP1-alpha dynamics that this isoform exists as a dimer a

168 ylated K9 H3 peptide in the aromatic cage of HP1 is only slightly affected by S10 phosphorylation, be

169 The chromodomain (CD) of HP1 proteins specifically recognizes the methyl mark on

170 oci marker and found that depleting cells of HP1 caused genotoxic stress, a delay in the repair of DS

171 Depletion of HP1, SUV39, SETDB1 or BRCA1 confer identical phenotypes.

172 ressing S473A was alleviated by depletion of HP1-beta, suggesting that phosphorylation of KAP-1 on Se

173 ve been elucidated, the molecular details of HP1 binding to H3K9me3 nucleosomes are unknown.

174 The chromoshadow domain of HP1 proteins promotes homodimerization, but this alone c

175 al borealin with the chromo shadow domain of HP1.

176 The chromo and chromo shadow domains of HP1(Hsalpha) play an essential role in HP1(Hsalpha)-nucl

177 Elimination of HP1, which "reads" H3K9me3, also caused major changes in

178 insights into the transcription functions of HP1, we sought to identify novel HP1-interacting protein

179 Each of the three human homologs of HP1 includes a chromoshadow domain (CSD).

180 al compaction states that are independent of HP1 phase separation.

181 n of heterochromatin foci are independent of HP1; (3) heterochromatin foci lack a separated liquid HP

182 Partial inhibition of HP1 family proteins accelerates the acquisition of segre

183 From inhibition of HP1-alpha homo-oligomerization we find the slow turn rat

184 We suggest that the dynamic interaction of HP1 with chromatin and other DDR factors could determine

185 t eukaryotes have at least three isoforms of HP1 that play differential roles in heterochromatin and

186 Because loss of HP1 caused redistribution of H3K27me2/3, but not H3K9me3

187 Abe et al. (2016), suggest that the means of HP1 localization and its function at inner centromeres a

188 Electron microscopy of HP1(Hsalpha)-associated nucleosomal arrays showed that H

189 AP-1 on Ser-473 promotes the mobilization of HP1-beta from heterochromatin and subsequent DNA repair.

190 dings that support the liquid-like nature of HP1 domains and discuss their functional implications in

191 hanism acts parallel with phosphorylation of HP1/Swi6 by CK2 to restrict Epe1.

192 Remarkably, a reduced proportion of HP1 bound to CPC is widespread in cancers, which causes

193 fect on total H3K9me3 levels, recruitment of HP1-gamma to promoters was lost.

194 on, demonstrating the spatial requirement of HP1 in CPC localization to the centromere.

195 In contrast, the role of HP1 in colon cancer has not been elucidated, and a mecha

196 Differential sedimentation of HP1(Hsalpha)-associated nucleosomal arrays showed that H

197 The present crystal structure of HP1 shows a remarkably straight helical stack involving

198 Notably, all three subtypes of HP1 seemed to be almost equally important for these DDR

199 Asp-394 on TM8 and Arg-276 on HP1 emerge as key residues that promote the reorientatio

200 calization with paxillin in cells is lost on HP1 mutation.

201 to constitutive heterochromatin in DCDC- or HP1-deficient mutants, and introduction of an H3K27 miss

202 ups, HP2-2 as reference, were as follows: OR HP1-1 0.73, 95% CI 0.34 to 1.56 (p value=0.41) and OR HP

203 Surprisingly, loss of either H3K9me3 or HP1 had only mild effects on heterochromatin compaction,

204 Knockdown of TBX2, KAP1 or HP1 inhibited NDRG1 promoter decoration specifically wit

205 shes nucleolar associations, whereas PCNA or HP1 interaction sites within p150 are not required for t

206 " in higher eukaryotes (e.g., by Polycomb or HP1) follows similar rules [4, 5] and note where such ef

207 S boundary, Orc3 facilitates assembly of ORC/HP1 proteins to chromatin.

208 Although HPL-2, like other HP1 orthologs, binds H3K9me peptides in vitro, the distr

209 (Fluorescent proteins, actin, tubulin, p53, HP1).

210 ighly conserved FAK-like hydrophobic pocket (HP1) abrogates CCM3-CCM2 interaction.

211 ere we show that binding of the key S. pombe HP1 protein, Swi6, to methylated nucleosomes drives a sw

212 Using the S. pombe HP1 protein, Swi6, we show that recognition of H3K9-meth

213 re, we use the two Schizosaccharomyces pombe HP1 paralogs, Swi6 and Chp2, as model systems to compare

214 compaction by the Schizosaccharomyces pombe HP1 protein Swi6 results in phase-separated liquid conde

215 ne such property, late replication, precedes HP1 recruitment, is under the control of zygotic transcr

216 gest that, rather than being a prerequisite, HP1-dependent heterochromatin antagonizes de novo centro

217 In contrast to previous proposals, HP1 can neither initiate nor accommodate neurotransmitte

218 r p21 expression and heterochromatin protein HP1-gamma phosphorylation.

219 d pRb interacts with heterochromatin protein HP1.

220 ue of the metazoan HETEROCHROMATIN PROTEIN1 (HP1) protein family.

221 h the architectural heterochromatin proteins HP1, DEK1, and ATRx, and was required for their localiza

222 sed in transport studies (i.e., PSA-HM1, PSA-HP1, PSA-HS2, and H3/49).

223 Two phages PSA-HP1, PSA-HS2 (Podoviridae and Siphoviridae) exhibited si

224 Remarkably, the PTVML HP1-binding site is embedded in the recently identified

225 A PXVXL HP1-interacting domain identified at position 487-491 of

226 leosome arrangement synergistically regulate HP1 function.

227 d chromatin restructuring via Chk2-regulated HP1-beta exchange from heterochromatin, promoting DNA re

228 P/GRPR signaling specifically down-regulates HP1(Hsbeta) expression and that inhibiting GRPR signalin

229 terochromatin, compaction, late replication, HP1 binding, and aggregation at the chromocenter, in suc

230 In breast cancer and melanoma, respectively, HP1(Hsalpha) and HP1(Hsbeta) have been shown to modulate

231 on of compacted chromatin in phase-separated HP1 droplets, which are dissolved or formed by specific

232 re are two putative hairpin-loop structures, HP1 and HP2.

233 y active heterochromatin, whereas Chp2/Swi6 (HP1 homologs) are recruited during the inactive state.

234 which binds to heterochromatin protein Swi6(HP1) across silenced chromosomal domains and to surround

235 2 and Clr3 and the chromodomain protein Swi6(HP1) are required for H3K9me spreading from nucleation s

236 ers transcripts to centromeres, whereas Swi6(HP1)-bound transcripts are evicted from chromatin and de

237 iscovered that RNAi and Sir2 along with Swi6(HP1) operate in two independent pathways to maintain het

238 in of Epe1 disrupt its interaction with Swi6(HP1) suggesting that this domain might have other functi

239 erminus of Epe1 directly interacts with Swi6(HP1), and H3K9 methylation stimulates this protein-prote

240 sion is promoted by the dissociation of Swi6/HP1 and cohesin Rad21 from telomeres, whereas disjunctio

241 9 in histone H3 (H3K9), which recruits Swi6/HP1 proteins to heterochromatic loci.

242 The Swi6/HP1-H3K9me3 chromatin complex lies at the center of hete

243 tions that disrupt its association with Swi6/HP1 fail to localize to heterochromatin, lead to accumul

244 non, increasing the specificity of targeting HP1-HMT complexes to gene promoters.

245 Mutation of the TBX2 HP1 binding domain abrogates the TBX2-HP1 interaction an

246 e TBX2 HP1 binding domain abrogates the TBX2-HP1 interaction and loss of repression of target genes s

247 establishment of H3K9me3 induced by tethered HP1.

248 Our findings demonstrate that HP1(Hsalpha)-nucleosome interactions cause chromatin con

249 suppression, providing direct evidence that HP1-HMTs act in a sequence-specific manner to achieve th

250 r promote chromatin compaction, we find that HP1-alpha dimers spatially redistribute to favor fast (5

251 These results indicate that HP1 is an essential modulator for CPC function and ident

252 the distribution of H3K27me, indicating that HP1 is important for normal localization of facultative

253 mical and proteomic approaches revealed that HP1 interacts with the histone chaperone complex FACT (f

254 We show that HP1(Hsalpha) preferentially binds histone H3K9Me3-contai

255 a)-associated nucleosomal arrays showed that HP1(Hsalpha) caused nucleosome associations within an ar

256 a)-associated nucleosomal arrays showed that HP1(Hsalpha) promotes interactions between arrays.

257 Therefore, our results suggest that HP1 binding by INCENP or Sgo1 is dispensable for centrom

258 lobal histone deacetylation and suggest that HP1-associated histone chaperone promotes nucleosome occ

259 The HP1 allele is hypothesised to improve outcome after spon

260 The HP1 C-terminal extension enhances the affinity, as does

261 The HP1 loop mutant also exhibited less biofilm formation th

262 histone H3K9 methyltransferase Clr4 and the HP1 proteins Swi6 and Chp2, as well as the two catalytic

263 e pre-mRNA associated factor NRDE-2, and the HP1-like protein HPL-2.

264 Ds) of the H3K9 methylase Suv39/Clr4 and the HP1/Swi6 protein.

265 (HP1-1 and HP2-1) and HP2-2 to evaluate the HP1 allele.

266 methylated nucleosomal templates and how the HP1-nucleosome complex achieves functional versatility r

267 cription and augment RNAII expression in the HP1 loop mutant.

268 characterized a phosphorylation site in the HP1-binding domain of KAP-1, Ser-473, which is phosphory

269 raphically defined complex that involves the HP1 chromodomain and an H3 tail peptide.

270 ge in the context of wild-type levels of the HP1 and Mod(mdg4) proteins might be part of an adaptive

271 Sgo1 interactions require the binding of the HP1 chromo shadow domain to PXVXL/I motifs in INCENP or

272 in AWC, we observe increased binding of the HP1 homolog HPL-2 at the odr-1 locus in AWC and reduced

273 Transversion mutation of the HP1 loop produced a smaller amount of sarA P3 and P2 tra

274 Here we probe how oligomerisation of the HP1-alpha isoform modulates interaction with chromatin,

275 also displayed defective mobilization of the HP1-beta chromodomain protein.

276 ously reported that dephosphorylation of the HP1-like protein Pdd1p is required for the formation of

277 in zebrafish embryos demonstrating that the HP1 interaction is essential for KDM2A function.

278 We further demonstrate that the HP1-binding site in TIN2 is required for sister telomere

279 atin and effector complexes that bind to the HP1 chromoshadow domain.

280 oincidentally, the Suv39/Clr4 CD, unlike the HP1/Swi6 CD, has been shown to prefer the trimethyl stat

281 vidual tyrosines to cation binding using the HP1 chromodomain as a model system.

282 we isolated proteins that interact with the HP1/ORC-associated protein (HOAP) capping protein, and i

283 rochromatin and the tunable dynamics of this HP1 isoform.

284 ruited to H3K9me3-modified chromatin through HP1, and HP1 can be recruited to unmodified chromatin by

285 hering the corresponding enzyme complexes to HP1-coated chromatin, thereby placing them in proximity

286 Direct binding of both Orc1 and Orc3 to HP1 suggests that, after the degradation of Orc1 at the

287 copy number variation (CNV), giving rise to HP1 and HP2 alleles, which influence haptoglobin express

288 In fission yeast, the two HP1 proteins Chp2 and Swi6 assume distinct roles and Chp

289 lmost all key NSC genes are switched off via HP1-mediated repression.

290 Distribution of HP CNV genotype was: HP1-1 n=132 (18.1%); HP2-1 n=342 (46.8%); and HP2-2 n=25

291 eveal for the first time a mechanism whereby HP1 is recruited to promoters by a well characterized Kr

292 urrent knowledge supports a paradigm whereby HP1 proteins achieve repression by binding to H3K9me mar

293 spread in vivo by reducing competition with HP1 proteins for the more prevalent dimethyl state.

294 in heterochromatin through cooperation with HP1 proteins.

295 state of ACMs through their interaction with HP1-gamma to direct heterochromatin formation and silenc

296 We show that TBX2 interacts with HP1 through a conserved HP1-binding motif in its N-termi

297 rs1 directly and specifically interacts with HP1/Swi6.

298 f KAP1, a master co-repressor, together with HP1 and the SETDB1 histone methyltransferase, which resu

299 e repeats, suggesting that ORC together with HP1 proteins may be involved in organizing higher-order

300 In fission yeast, HP1 proteins Chp2 and Swi6, which bind to methylated his