ライフサイエンスコーパス: HSQC

1 HSQC analysis overcame this problem.

2 HSQC and GC-MS then jointly guided purification and stru

3 HSQC experiments performed in organic solvents and deter

4 HSQC spectra of the enzyme bound to cognate (specific) a

5 trained on a database containing over 2,054 HSQC spectra as the training set.

6 Two-dimensional 1H-13C HSQC (heteronuclear single quantum correlation) and fast

7 we have recorded ultrahigh-resolution 1H-13C HSQC NMR spectra of cell extracts, which exhibit spectra

8 Moreover, both CD and 1H-13C HSQC NMR studies reveal that these short peptoid helices

9 ows recording of ultrahigh resolution 1H-13C HSQC spectra in a fraction of the time needed for record

10 hydrogen dimension from heteronuclear 1H-13C HSQC spectroscopy, which did not detect resonances for t

11 ample we measured a natural abundance 1H-13C HSQC spectrum of metabolites from granulocyte cell extra

12 We show that a high-resolution 1H-13C HSQC spectrum with 4k complex increments recorded linear

13 the experiments of TOCSY, NOESY, ROESY, 13C HSQC 2D NMR, and ESI-MS and GC.

14 15N HSQC NMR and CD spectral analyses were employed to chara

15 15N HSQC NMR spectra of both the apo and Zn(II) proteins rev

16 ion in intensity and disappearance of 1H-15N HSQC cross-peaks, were observed with the addition of eit

17 identifies the sequence positions of 1H-15N HSQC cross-peaks.

18 ased on 1H-1H NOESY and heteronuclear 1H-15N HSQC high-resolution NMR spectra.

19 Using two-dimensional 1H-15N HSQC NMR analysis, we demonstrate that C-terminal peptid

20 intermediate species populate the 2D 1H-15N HSQC NMR spectra between pH 4 and 5.

21 ubset of the p53TAD residues that had 1H-15N HSQC resonance intensity reductions during the complex f

22 CSP) data routinely obtained using 2D 1H-15N HSQC spectra in high-throughput ligand affinity screens.

23 Two-dimensional 1H-15N HSQC spectra were recorded for 15N- and 13C-labeled muri

24 trate binding monitored by changes in 1H-15N HSQC spectra yielded a dissociation constant for the bin

25 exchange broadening of cross-peaks in 1H-15N HSQC spectra.

26 sponding large intensity reduction in 1H-15N HSQC spectra.

27 affinity b-MBD is readily observed by 1H-15N HSQC spectroscopy.

28 In addition, the 1H-15N HSQC spectrum taken at 1.5 M guanidinium chloride reveal

29 s of NMR experiments: two-dimensional 1H-15N HSQC titrations of backbone NH and of Arg N epsilonH res

30 protons in successive acquisitions of 1H-15N HSQC-NOESY-HSQC spectra provides the first direct observ

31 gomerization process was monitored by 1H/15N HSQC NMR experiments, which provided the first residue-s

32 the resonances in the two-dimensional 1H/15N HSQC spectrum of uniformly 15N labeled Vpu(2-30+) in mic

33 Ubiquitin resonances were monitored by 15N HSQC NMR experiments at varying temperatures and salt co

34 15N-HSQC NMR spectra of betaK87T and betaE109D mutant Trp sy

35 aD305A Trp synthase mutant only showed a 15N-HSQC signal in the presence of disodium alpha-glyceropho

36 Chemical shift perturbations observed in 15N-HSQC spectra upon the addition of CoA indicated that the

37 No 15N-HSQC signal was detected for 1-15N-L-Trp in 10 mm trieth

38 um coherence nuclear magnetic resonance (15N-HSQC NMR) spectroscopy for the presence of 1-15N-L-Trp b

39 Our results demonstrate that the 15N-HSQC NMR spectra of 1-15N-L-Trp bound to Trp synthase ca

40 ing and shifting observed in the 2D-{1H,15N}-HSQC-monitored titrations of 15N-Phe-labeled P450(eryF)

41 Most significantly, 15N-1H HSQC experiments showed that temperature-dependent shift

42 The 15N-1H HSQC NMR spectrum of the human alpha-lactalbumin (alpha-

43 rse relaxation-optimized spectroscopy 15N-1H HSQC spectroscopy of apo-AChBP revealed seven well resol

44 a dramatic sharpening of peaks in the 15N-1H HSQC spectrum of human alpha-LA at pH 2.

45 were characterized by (1)H and (13)C NMR, 2D HSQC and COSY NMR, mass spectrometry, and elemental anal

46 KmTx9 (3)] was achieved through overlaid 2D HSQC NMR techniques, while the relative configurations w

47 y using fitted cross-peaks of a reference 2D HSQC experiment as footprints, which are subsequently us

48 y gain of about 40-50% over a traditional 2D HSQC-SI experiment.

49 copy to extract chemical information from 2D-HSQC spectra, termed HSQC correlation spectroscopy (HSQC

50 and sensitive NMR experiments (1D-proton, 2D-HSQC) for the unique identification of known flavones, f

51 longed multidimensional, so-called pseudo-3D HSQC experiments where the pseudo dimension is a radio f

52 Furthermore, a HSQC spectrum collected with a multiplicity-edited pulse

53 tation decoupling) to data acquisition in an HSQC experiment causes broadband homonuclear decoupling,

54 th conventional and accelerated DQF-COSY and HSQC.

55 roups is demonstrated by (1)H-(15)N HMBC and HSQC NMR analysis.

56 measurements such as COSY, NOESY, HMQC, and HSQC experiments are central to small-molecule and biomo

57 In addition, (15)N-(1)H HSQC and HSQC-filtered NOESY spectra carried out with a duplex ha

58 Calorimetric measurements and HSQC NMR spectra confirm that the engineered variants ar

59 (13)C NMR and HSQC spectra confirmed the presence of pectic- and gluco

60 Conventional two-dimensional NMR, such as HSQC experiments, can provide residue-specific informati

61 in small molecule NMR spectroscopy, such as HSQC, HMQC, HMBC, COSY, NOESY, TOCSY, and similar, can b

62 A novel NMR experiment, the so-called ASAP-HSQC, is introduced that allows the detection of heteron

63 ialysis procedure, signals were monitored by HSQC NMR.

64 e (1)H and (15)N NMR resonances, obtained by HSQC experiments, are shown to differentiate subunits an

65 ption FTIR, operando UV/Vis and (1) H-(13) C HSQC NMR spectroscopy indicate that activity arises from

66 per mil) can be reached with the (1)H-(13)C HSQC (Heteronuclear Single Quantum Correlation) experime

67 ntly introduced 2D real-time BIRD (1)H-(13)C HSQC experiment for NMR-based metabolomics of aqueous sa

68 With the use of high-resolution (1)H-(13)C HSQC experiments, complexes of amphiphiles with more tha

69 spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the lat

70 cid binding protein (I-BABP), and (1)H-(13)C HSQC spectra were recorded to show the utility of the co

71 been studied using (13)C NMR and (1)H-(13)C HSQC spectroscopy.

72 The (1)H-(13)C HSQC spectrum of p51 obtained at micromolar concentratio

73 uclear couplings ((1)H-(1)H COSY; (1)H-(13)C HSQC), in conjunction with high-level structural DFT cal

74 (1)H-(1)H COSY, (1)H-(1)H NOESY, (1)H-(13)C HSQC, and (1)H-(13)C HMBC spectroscopy, absorption spect

75 h two-dimensional (1)H-(1)H COSY, (1)H-(13)C HSQC, and (1)H-(19)F HETCOR NMR techniques, the solution

76 shift changes in (1)H-(15)N HSQC, (1)H-(13)C HSQC, and (19)F NMR spectra of the different single site

77 le quantum correlation (HSQC) and (1)H-(13)C HSQC.

78 Here, we present (1)H/(13)C-HSQC spectra of protonated methyl groups in a model syst

79 beled at one subunit and obtained (1)H/(13)C-HSQC spectra of this assembly.

80 s represented by a doublet in the (1)H,(13)C-HSQC spectrum.

81 ased S/N compared to the standard (1)H,(13)C-HSQC), as shown for resonance distinction and unambiguou

82 ((1)H,(1)H-COSY, (1)H,(1)H-NOESY, (1)H,(13)C-HSQC, (1)H,(13)C-HMBC) NMR spectroscopies, and structure

83 les including circular dichroism, (1)H,(13)C-HSQC, and (1)H,(1)H-NOESY 2D-NMR studies, validated the

84 etection scheme, the selective Halpha,Calpha-HSQC (SHACA-HSQC), using extensive hetero- and homonucle

85 residues) can be achieved with a single CaN HSQC experiment.

86 NMR heteronuclear single quantum coherence (HSQC) analyses reveal the 6-strand beta-sheet face of Cr

87 and heteronuclear single quantum coherence (HSQC) are also demonstrated.

88 15)N heteronuclear single quantum coherence (HSQC) as well as one-dimensional (1)H and (19)F NMR to s

89 -15N heteronuclear single quantum coherence (HSQC) correlation experiment are much broader than those

90 the heteronuclear single quantum coherence (HSQC) experiment.

91 13)C heteronuclear single quantum coherence (HSQC) HR-MAS NMR can provide rapid analysis of the cell

92 (1)H heteronuclear single-quantum coherence (HSQC) in conjunction with other two-dimensional (2D) NMR

93 -15N heteronuclear single quantum coherence (HSQC) NMR and inductively coupled plasma mass spectromet

94 NUS heteronuclear single quantum coherence (HSQC) NMR pulse sequence was adapted to a state-of-the-a

95 15)N heteronuclear single-quantum coherence (HSQC) NMR spectra from 0 to 6.5 M urea under equilibrium

96 n 2D heteronuclear single quantum coherence (HSQC) NMR spectra of the entire array of wall polymers.

97 1)H]-heteronuclear single quantum coherence (HSQC) NMR spectroscopy.

98 15)N-heteronuclear single-quantum coherence (HSQC) NMR studies with a di-domain (lipoyl domain+ linke

99 Heteronuclear single quantum coherence (HSQC) NMR titrations indicate that these compounds inter

100 OSY) heteronuclear single-quantum coherence (HSQC) NMR to characterize its binding site as a pocket a

101 Heteronuclear single quantum coherence (HSQC) NMR was then used to map out the binding surface,

102 15)N heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) revealed that str

103 15N} heteronuclear single quantum coherence (HSQC) show that these chiral ILs exhibit intramolecular

104 15)N-heteronuclear single quantum coherence (HSQC) spectra of CaaD showed seven to nine Arg-NepsilonH

105 15)N heteronuclear single quantum coherence (HSQC) spectra of wild-type and mutant CzrAs reveal that

106 15)N heteronuclear single quantum coherence (HSQC) spectra, limited proteolytic digestion, and fluore

107 Heteronuclear single quantum coherence (HSQC) spectroscopy shows that both peptides bind in the

108 -15N heteronuclear single quantum coherence (HSQC) spectroscopy, is highly cooperative.

109 -15N heteronuclear single quantum coherence (HSQC) titration experiment was performed on a 15N-labele

110 15)N-heteronuclear single quantum coherence (HSQC) titrations of this kinase domain with the RBDs.

111 e of heteronuclear single quantum coherence (HSQC) to measure SCB in a complicated system.

112 15)N heteronuclear single quantum coherence (HSQC)) NMR.

113 nts (heteronuclear single-quantum coherence (HSQC)) of (13)C-labeled tunicamycin enriched from D-[1-(

114 d by heteronuclear single quantum coherence (HSQC), nuclear magnetic resonance (NMR), and py-GC/MS.

115 sing heteronuclear single quantum coherence (HSQC)-based NMR techniques.

116 and heteronuclear single quantum coherence, HSQC) nuclear magnetic resonance spectra of undiluted wi

117 l experiment time compared to a conventional HSQC.

118 nd in two-dimensional (13)C-(1)H correlated (HSQC) NMR spectra of lignins isolated from cinnamoyl CoA

119 5)N heteronuclear single-quantum correlated (HSQC) spectra for both wild-type K3 and mutated [r(K57D)

120 rotein's [15N-1H]-heteronuclear correlation (HSQC) spectrum recorded under conditions generally suita

121 MR heteronuclear single quantum correlation (HSQC) analysis revealed chemical perturbations after tit

122 )P heteronuclear single quantum correlation (HSQC) and (1)H-(13)C HSQC.

123 )H heteronuclear single quantum correlation (HSQC) and provides an additional chromatographic-like se

124 )N heteronuclear single-quantum correlation (HSQC) experiments, we were also able to examine the inte

125 )N heteronuclear single quantum correlation (HSQC) experiments.

126 nd heteronuclear single quantum correlation (HSQC) NMR spectra confirmed that the beta-trefoil global

127 of heteronuclear single-quantum correlation (HSQC) spectra even allowed for the discovery of a new tr

128 5N heteronuclear single quantum correlation (HSQC) spectra in water-alcohol mixed solvents and observ

129 A heteronuclear single quantum correlation (HSQC) spectrum of R*-generated GTPgammaS/Mg(2+)-bound Ch

130 1H heteronuclear single quantum correlation (HSQC) spectrum.

131 , 1D (1)H and (13)C NMR, as well as 2D COSY, HSQC, HSQC-TOCSY, and HMBC spectra.

132 D ((1)H NMR and (13)C NMR) and 2D-NMR (COSY, HSQC and NOESY) spectral analysis.

133 D ((1)H NMR and (13)C NMR) and 2D-NMR (COSY, HSQC and NOESY) spectral studies.

134 n this paper, a nonrefocused (1)H,(15)N CPMG HSQC of uniformly (13)C,(15)N-labeled 33-mer PEMV-1 RNA

135 (1)H TOCSY and (1)H-(13)capital ES, Cyrillic HSQC spectra.

136 g the dual carbon label selective HSQC (DCLS-HSQC) pulse sequence and exploiting differences in 1J 15

137 mension of heteronuclear broadband decoupled HSQC (heteronuclear single quantum correlation) spectra.

138 (1)H, inverse-gated (IG) (13)C, DEPT-HSQC NMR, and mass spectrometry (MS) experiments confirm

139 agments and examined them by two-dimensional HSQC NMR and fluorescence spectroscopies, by differentia

140 COSY, TOCSY and NOESY, and (1)H-(13)C edited HSQC spectroscopy.

141 he appearance of the (15)N- and (13)C-edited HSQC spectra, where line broadening of the same peptide

142 intensities for NH3 cross-peaks than either HSQC or heteronuclear multiple quantum coherence (HMQC)

143 es data from 3D 13C-edited, 13C/15N-filtered HSQC-NOESY spectra for evaluating ligand binding poses w

144 ng pose, the 3D 13C-edited, 13C/15N-filtered HSQC-NOESY spectrum is predicted, and the predicted and

145 changes in ChiT upon heterotrimer formation, HSQC spectra of the (15)N-ChiT-reconstituted heterotrime

146 nsional (2D) NMR spectra, namely, (13)C-(1)H HSQC (heteronuclear single quantum coherence spectroscop

147 of R69D with Ca(2+), monitored by (15)N-(1)H HSQC (heteronuclear single quantum coherence) NMR, showe

148 hen cocrystallized or analyzed by (15)N-(1)H HSQC (heteronuclear single-quantum coherence) NMR (nucle

149 spectra constructed from pairs of (13)C-(1)H HSQC and (13)C-(1)H HSQC-TOCSY spectra.

150 In addition, (15)N-(1)H HSQC and HSQC-filtered NOESY spectra carried out with a

151 For 2D (15)N-(1)H HSQC experiments, we can produce an exact solution using

152 persed amide crosspeaks in 2D NMR (15)N-(1)H HSQC fingerprint region, and rotational correlation time

153 Cys-83 was determined to be <6 by (13)C-(1)H HSQC NMR experiments with [3-(13)C]cysteine-labeled Zn(2

154 Two-dimensional (15)N-(1)H HSQC NMR spectra of intact LigBCen2 in the absence and p

155 Here, (15)N-(1)H HSQC NMR spectroscopy demonstrates that 0118 indeed targ

156 e differ, as defined by comparing (15)N,(1)H HSQC spectra of a (15)N-labeled model TM peptide in both

157 Similar (15)N-(1)H HSQC spectra were obtained in a variety of detergents, i

158 or covariance (1)H-(1)H TOCSY and (13)C-(1)H HSQC-TOCSY spectra and triple-rank correlation spectra c

159 g it to 2D (1)H-(1)H TOCSY and 2D (13)C-(1)H HSQC-TOCSY spectra of a cell lysate from E. coli, which

160 ions of 2D (1)H-(1)H TOCSY and 2D (13)C-(1)H HSQC-TOCSY spectra, it allows the straightforward and un

161 from pairs of (13)C-(1)H HSQC and (13)C-(1)H HSQC-TOCSY spectra.

162 al correlation spectroscopy), and (13)C-(1)H HSQC-TOCSY, for the comprehensive, accurate, and efficie

163 DI using (19)F ligand-observe and (15)N,(1)H-HSQC protein-observe NMR methods.

164 ligand-observe and complimentary (15)N,(1)H-HSQC titrations to monitor interactions from the protein

165 (15)N) NMR experiments and 2D [(13)C, (1)H] HSQC.

166 ity and signal dispersion of 2D [(13)C,(1)H]-HSQC offer new avenues to study challenging systems wher

167 were assigned by 2D 1H-15N heterocorrelated HSQC NMR spectroscopy, and the 15N-1H coupling constant

168 peaks from the two-dimensional heteronuclear HSQC spectrum of a sample of natural organic matter that

169 r TOCSY-based DemixC method to heteronuclear HSQC-TOCSY NMR spectroscopy.

170 Homonuclear (H,H COSY) and heteronuclear (HSQC) methods were developed, validated, and compared.

171 However, HSQC titrations monitoring Trp253 (located between monom

172 1)H and (13)C NMR, as well as 2D COSY, HSQC, HSQC-TOCSY, and HMBC spectra.

173 There were changes in HSQC-chemical shifts throughout the domain on binding fo

174 e broadening of the majority of the peaks in HSQC spectra except for the residues at the termini, pre

175 whose diverse composition causes signals in HSQC spectra to disperse.

176 substance of molecular mass <3 kDa, which is HSQC NMR silent, and is not taken up by the cell.

177 We report here the use of J-resolved HSQC (J-HSQC) for 13C isotopomer analysis of tissue samples.

178 ield gradient NMR diffusion experiments (LED-HSQC), measures hydrodynamic properties, or molecular si

179 and (7)Li NMR ((1)H NOESY, TOCSY, (1)H/(7)Li HSQC, and DO-NMR) studies on the solution structure of 3

180 reduce the number of overlaps in the 500 MHz HSQC spectrum from 10 to 1 using four samples with a tru

181 The (1)H-(15)N HSQC (heteronuclear single quantum correlation) solution

182 (1)H-(15)N HSQC analysis indicates that enzyme conformations in the

183 (1)H-(15)N HSQC and 3D (1)H-(15)N NOESY HSQC spectra of the F50Y mu

184 le synergy between NADH and PDC, (1)H- (15)N HSQC chemical shift perturbation and saturation transfer

185 We used two-dimensional (1)H,(15)N HSQC chemical shift perturbation mapping of (15)N-labele

186 nding of these ligands by mapping (1)H-(15)N HSQC chemical shift perturbations to our new NMR structu

187 The majority of the (1)H-(15)N HSQC cross-peaks of the folded state show only a limited

188 (1)H-(15)N HSQC data of nFGF-1, acquired in the denatured state(s)

189 pressure-dependent site-specific (1)H-(15)N HSQC data with coarse-grained molecular dynamics simulat

190 (1)H-(15)N HSQC experiments were collected at stoichiometric interv

191 pharmacophore-derived X-ray screen and (15)N HSQC NMR based KD determination to rapidly identify hits

192 ic of an unstructured peptide, and the (15)N HSQC NMR spectra of IA(3) were characteristic of a polyp

193 22 amide groups were observed in (1)H-(15)N HSQC NMR spectra.

194 ifts of the amino acids of CaM in (1)H-(15)N HSQC NMR spectra.

195 Here, (1)H-(15)N HSQC NMR spectroscopy is applied to PvuII endonuclease (

196 f 508 compounds and validation by (1)H-(15)N HSQC NMR spectroscopy led to the identification of a min

197 re, as there is no change in the (1)H- (15)N HSQC NMR spectrum in comparison to wild-type CaM.

198 A (1)H-(15)N HSQC NMR titration study with three different tropocolla

199 ring Ca(2+) binding, we have used (1)H-(15)N HSQC NMR to monitor more than 40 residues in Paramecium

200 millimolar affinities as determined by (15)N HSQC NMR.

201 different urea concentrations by (1)H-(15)N HSQC NMR.

202 titration calorimetry (ITC), and (1)H-(15)N HSQC NMR.

203 (1)H-(15)N HSQC nuclear magnetic resonance (NMR) spectroscopic stud

204 t-range distance information from (1)H-(15)N HSQC perturbation spectroscopy give strong indication fo

205 (1)H-(15)N HSQC spectra acquired for wt-, 10L-fCzrA and H97N 10L-fC

206 Both states exhibit similar (1)H-(15)N HSQC spectra and the same pattern of peptidyl-prolyl pep

207 onformation, and the solution NMR (1)H/(15)N HSQC spectra have a single well resolved resonance for e

208 ional proton, and two-dimensional (1)H-(15)N HSQC spectra of Ca(2+)-bound T3-Cterm indicate a distinc

209 Two-dimensional (1)H-(15)N HSQC spectra of mutated T3-Cterm showed little evidence

210 (1)H-(15)N HSQC spectra of the F50A mutant reveal widespread and la

211 (1)H-(15)N HSQC spectra showed largely intact protein structures fo

212 t despite some differences in the (1)H-(15)N HSQC spectra the two are nearly identical in NOE distanc

213 DCA) and successive heteronuclear (1)H-(15)N HSQC spectra were collected to identify the backbone ami

214 f chemical shift perturbations of (1)H-(15)N HSQC spectra, relaxation-dispersion experiments, and fil

215 he well-dispersed two-dimensional (1)H-(15)N HSQC spectrum in SDS micelles indicates that it is feasi

216 The NMR (1)H-(15)N HSQC spectrum of CH1-Zn(2+) exhibits few poorly disperse

217 ectroscopy was used to assign the (1)H-(15)N HSQC spectrum of the B6-hbeta2m complex, revealing that

218 tigation of the dependence of the (1)H-(15)N HSQC spectrum of the RNase H domain on [Mg(2+)] indicate

219 a-sheet assembly and interaction: (1)H,(15)N HSQC studies facilitate the identification of the monome

220 sistent with this kinetic scheme, (1)H-(15)N HSQC titration of MutT with dGMP reveals weak binding an

221 Chemical shift changes in (1)H-(15)N HSQC, (1)H-(13)C HSQC, and (19)F NMR spectra of the diff

222 mensional proton, two-dimensional (1)H-(15)N HSQC, and (19)F NMR spectroscopies were used to examine

223 the coassembly of the peptides by (1)H,(15)N HSQC.

224 In this work, we have used (15)N-HSQC based NMR titration experiments of a 12-residue pep

225 aride altered the two-dimensional (1)H-(15)N-HSQC spectra of CXCL12, which identified two clusters of

226 Comparison of the 2D (15)N-HSQC spectra of IA(3) in water and in 23% 2,2,2-trifluor

227 ring chemical shift changes in protein (15)N-HSQC spectra.

228 (1)H-(15)N-HSQC titration of the enzyme with the substrate analogue

229 een studied at 298 K, pH 5.3 by [(1)H,(15)N] HSQC 2D NMR spectroscopy.

230 each structure, a combination of (1)H[(15)N] HSQC and HMBC and (1)H COSY and NOESY NMR spectroscopy w

231 NS demonstrating the utility of [(1)H,(15)N] HSQC NMR spectra to provide a spectroscopic fingerprint

232 amine-DNA interaction using 2D [(1)H, (15)N] HSQC NMR spectroscopy allows study of the role of the li

233 -specific assignments using the [(1)H,(15)N] HSQC-TOCSY experiment.

234 particularly robust H-bonds, and [(1)H-(15)N]HSQC-NOESY spectra lead to the identification of three a

235 2D [(1)H-(15)N]-HSQC NMR was used to follow conformational changes upon

236 [(1)H-(15)N]-HSQC spectra confirm the functionalities of several key

237 Kd of 4.4 nM, as shown by SPR, [(1)H,(15)N]-HSQC, and (19)F NMR.

238 ree ligands as monitored by 2D {(1)H, (15)N}-HSQC NMR spectroscopy.

239 quantitative yeast two-hybrid, BIAcore, NMR HSQC and STD, and confocal analyses that amino acids phe

240 ts as assessed by poor expression and/or NMR HSQC experiments, while more conservative mutations at t

241 ometry results were validated using STD-NMR, HSQC-NMR, and ITC experiments.

242 (1)H-(15)N HSQC and 3D (1)H-(15)N NOESY HSQC spectra of the F50Y mutant demonstrate its conforma

243 single-crystal X-ray analysis and 2D (NOESY, HSQC, HMBC) NMR experiments.

244 SO-d(6) has been assigned using COSY, NOESY, HSQC, and HMBC NMR methods.

245 NOESY-HSQC 3D-NMR and NOESY 2D-NMR techniques have been used o

246 cted with a long mixing-time 1H-1H-15N NOESY-HSQC spectrum confirmed the formation of four clusters.

247 eronuclear single quantum correlation (NOESY-HSQC) NMR, in which the exocyclic amines at X(7) or Y(19

248 successive acquisitions of 1H-15N HSQC-NOESY-HSQC spectra provides the first direct observation of th

249 nts obtained from isotope-edited (15)N NOESY-HSQC data indicated that the (R)-gamma-hydroxytrimethyle

250 ues, so that assignments were based on NOESY-HSQC data and on the response to paramagnetic Co(2+) add

251 It is shown that in a VRT-NOESY-HSQC 3D experiment in 10 mM (13)C natural-abundance sucr

252 ith (13)CH(3)epsilon-methionine and obtained HSQC spectra of unliganded receptor as well as receptor

253 carbon resonance assignments, expansions of HSQC-spectra, HPLC parameters (retention time, relative

254 sion-ordered spectroscopy (DOSY): (1)H-(31)P HSQC-DOSY.

255 xperiments and a heteronuclear 2D (1)H-(31)P HSQC-NOESY experiment by taking advantage from spin diff

256 includes the application of a 2D (1)H-(31)P HSQC-TOCSY experiment to detect (31)P-labeled metabolite

257 dimensional (1D) (31)P NMR and 2D (1)H-(31)P HSQC-TOCSY spectra of 38 common phosphorus-containing me

258 The quantitative, QEC-HSQC experiment was also found to be very promising for

259 d quantitative heteronuclear single quantum (HSQC) (1)H-(13)C NMR spectra.

260 We report here the use of J-resolved HSQC (J-HSQC) for 13C isotopomer analysis of tissue samp

261 A robust J-resolved HSQC experiment affording highly resolved (1)JCH/(1)TCH

262 uantum coherence nuclear magnetic resonance (HSQC NMR) spectroscopy revealed robust helical folding p

263 uantum coherence-nuclear magnetic resonance (HSQC-NMR) or, following suitable derivatization, by gas

264 is of one- and two-dimensional (COSY, ROESY, HSQC, and HMBC) experiments.

265 py analyses including (1)H-NMR; COSY; ROESY; HSQC and HMBC.

266 d larger (S/N)(t) than for the (1)H-(13)C SE-HSQC reference sequence is achieved, for the sigma(32) p

267 A high-resolution band-selective HSQC experiment was developed to identify (13)C NMR sign

268 By using the dual carbon label selective HSQC (DCLS-HSQC) pulse sequence and exploiting differenc

269 SHACA-HSQC spectra acquired on IDPs provide uncompromised reso

270 eme, the selective Halpha,Calpha-HSQC (SHACA-HSQC), using extensive hetero- and homonuclear decouplin

271 ion, by applying Spectral Aliased Pure Shift HSQC, the analysis was enhanced with the determination o

272 Appropriately pH-shifting HSQC NMR peaks were identified in the (13C)aziridine-mod

273 (29)Si NMR and, with the aid of (1)H-(29)Si HSQC, were assigned by comparison with parent compounds

274 Side-by-side HSQC comparisons of crude P450 extracts against a contro

275 ear single quantum correlation spectroscopy (HSQC) NMR spectrum of the non-covalent complex showed th

276 ear single-quantum correlation spectroscopy (HSQC)-NOESY.

277 med via spectroscopic analysis (CD spectrum, HSQC, COSY, and ROESY NMR experiments).

278 cal information from 2D-HSQC spectra, termed HSQC correlation spectroscopy (HSQCcos), is reported.

279 The HSQC pulse sequences are ideal for collecting high-quali

280 The HSQC pulse sequences, on the other hand, required roughl

281 The HSQC spectra of GlcNS, fondaparinux, and the low-molecul

282 The HSQC spectral changes indicate the structures of both C(

283 The HSQC spectrum of (15)N-ChiT in this complex displays a u

284 only minor changes are observed between the HSQC spectra of the two ACP species and no NOEs are obse

285 In contrast, the HSQC NMR spectrum of the covalent complex showed that th

286 Well-defined shifts in peaks in the HSQC spectrum of (15)N labeled NusE/NusB when the unlabe

287 A subset of cross-peaks in the HSQC spectrum of p23 is shifted upon addition of the mid

288 Analysis of the HSQC NMR peak intensity for 4 in the presence of differe

289 resonance overlap in crowded regions of the HSQC spectra hampers accurate metabolite identification

290 Similarly, the HSQC spectrum of 15N-labeled N domain is unperturbed by

291 Database query is performed using the HSQC spectrum, and the top metabolite hits are then vali

292 elative percent congestion by 84.9% in their HSQC spectra using only four samples.

293 -H cross-peaks measured with a constant time HSQC experiment without and with J(C[bond]N) amplitude m

294 T approaches can be transferred from HMQC to HSQC-type experiments.

295 Here we propose the use of a 3D TOCSY-HSQC experiment for (13)C-MFA.

296 cytochrome b5 concentration by 1H-15N TROSY-HSQC experiments.

297 s a function of K+ concentration by 2D-TROSY-HSQC in both camphor-bound oxidized (CYP-S) and camphor-

298 Finally, a comparison of the TROSY-HSQC 2D NMR spectra of wild-type sfALR and its R194H mut

299 dynamic structural measurements using TROSY-HSQC NMR spectroscopy.

300 Extrapolated time-zero HSQC was applied using DMSO-d6/[Emim]OAc-d14 and enabled