ライフサイエンスコーパス: IGFBP-2

1 nsulin-like growth factor binding protein 2 (IGFBP-2).

2 hybridization to determine the expression of IGFBP-2.

3 yze the structure of the IGF-binding site on IGFBP-2.

4 g domain is located within the C terminus of IGFBP-2.

5 ges were rescued following administration of IGFBP-2.

6 f the CWCV motif (residues 247-250) in human IGFBP-2.

7 tion and dissociation rates than full-length IGFBP-2.

8 hyroglobulin-RGD region of the C terminus of IGFBP-2.

9 1.16; 95% CI: 1.08-1.24; P(trend) < 0.001), IGFBP-2 (1.18; 1.06-1.31; P(trend) < 0.01) and IGFBP-3 (

10 al domain had no further effect on affinity (IGFBP-2(1-190) EC50 = 9.2 nM).

11 In kinetic assays, IGFBP-2(1-248) and IGFBP-2(1-190) exhibited more rapid association and diss

12 FBP-2(1-248) and IGFBP-2(249-289) as well as IGFBP-2(1-190) were expressed as glutathione S-transfera

13 binding affinity (IGFBP-2 EC50 = 0.35 nM and IGFBP-2(1-248) = 7 nM).

14 In kinetic assays, IGFBP-2(1-248) and IGFBP-2(1-190) exhibited more rapid a

15 (BNPS-skatole) and the BNPS-skatole products IGFBP-2(1-248) and IGFBP-2(249-289) as well as IGFBP-2(1

16 t free IGF-1 and a positive association with IGFBP-2 (11.9 ng/mL; 95% CI 5.0; 18.8).

17 the BNPS-skatole products IGFBP-2(1-248) and IGFBP-2(249-289) as well as IGFBP-2(1-190) were expresse

18 studies revealed that normal RPE can produce IGFBP-2, -3, -4, -5, and -6 but not IGFBP-1.

19 ogressive increases in message abundance for IGFBP-2, -3, -4, and -6.

20 Likewise, although IGFBP-2, -3, and -5 mRNA levels fell, membrane-associate

21 IGFBP-2, -3, and -5 were effective inhibitors of both li

22 ncreased message abundance and production of IGFBP-2, -4, -5, and -6, but not IGFBP-3.

23 roliferation with coaddition of 20 or 100 nM IGFBP-2 (50 or 80% inhibition, respectively).

24 and shows high sequence identity with human IGFBP-2 (52%).

25 IGFBP-2, a regulator of insulin-like growth factor actio

26 We also tested the ability of IGFBP-2, a related binding protein which has an arginine

27 An anti-IGFBP-4Ab, but not an anti-IGFBP-2 Ab or normal rabbit serum, shifted the [125I] IG

28 vels of GH, IGF-1 and IGF-binding protein 2 (IGFBP-2) after gastric sleeve surgery in healthy obese i

29 on MCF-7 breast cancer cells, we found that IGFBP-2 also regulates PTEN.

30 Among the other IGFBPs, the N-domains of IGFBP-2 and -3 also had strong transactivation activity,

31 at are critical for transactivation and that IGFBP-2 and -3 also have strong transactivation activity

32 IGFBP-2 and -3, the major binding proteins identified in

33 like growth factor-binding proteins (IGFBP), IGFBP-2 and -4, and that these IGFBPs modulate IGF-I-sti

34 d porcine vitreous contain two major IGFBPs, IGFBP-2 and an approximately 29-kDa fragment of IGFBP-3.

35 We synthesized a HBD peptide specific for IGFBP-2 and demonstrated in vitro that it rescued the mi

36 novel mechanism for coordinate regulation of IGFBP-2 and IGF-I signaling functions that lead to stimu

37 STC2 was inversely related to total IGF-1, IGFBP-2 and IGFBP-3 and positively to IGFBP-1.

38 IGFBP-binding domain on IGF-1 in binding to IGFBP-2 and IGFBP-3 and support involvement of the IGF-1

39 In BIAcore analysis, IGFBP-2 and IGFBP-3 bound only to the N(epsilon)(Lys65/6

40 igh affinity ( approximately 0.1-0.2 nM) for IGFBP-2 and IGFBP-3 in solid-phase-binding assays.

41 and cross-sectional insulin, IGF-I, IGF-II, IGFBP-2 and IGFBP-3 in the Boyd Orr cohort (n = 182 men,

42 Lower preoperative IGFBP-2 and IGFBP-3 levels and biopsy Gleason score were

43 ns of IGF-1 involved in the interaction with IGFBP-2 and IGFBP-3, we employed IGF-1 selectively bioti

44 ies were observed in ligand blot analysis of IGFBP-2 and IGFBP-3.

45 y of Gly 1 and Lys 27 when IGF-1 is bound to IGFBP-2 and IGFBP-3.

46 he effects of individual IGFBPs were tested, IGFBP-2 and IGFBP-4 were found to inhibit IGF-I-stimulat

47 SF levels of IGFBP-2 and IL-6 did not differ by treatment group.

48 gene, IIp45, whose protein product binds to IGFBP-2 and inhibits glioma cell invasion.

49 The link between IGFBP-2 and PTEN was of particular interest because elev

50 We investigated the role of IGFBP-2 and receptor protein tyrosine phosphatase beta (

51 spermidine and spermine elevated endogenous IGFBP-2 and SSAT mRNA abundance, whereas, putrescine sti

52 identity of the approximately 35-kDa band as IGFBP-2 and the approximately 29-kDa band as a fragment

53 rtilage, and ERT increases the production of IGFBP-2 and the synthesis of PGs by chondrocytes from su

54 and regeneration, including PDGF-A, IGFBP-3, IGFBP-2, and IGF-1.

55 ally important roles for circulating IGF-II, IGFBP-2, and IGFBP-3 in PSA-detected prostate cancer, in

56 d measurements of blood serum IGF-I, IGF-II, IGFBP-2, and IGFBP-3 obtained at recruitment.

57 Plasma levels of IGF-I, IGFBP-2, and IGFBP-3 were measured preoperatively in 120

58 an and porcine vitreous, plasma, recombinant IGFBP-2, and IGFBP-3 were separated by gel electrophores

59 ions of native IGF-I, native IGFBP-1, native IGFBP-2, and their respective analogues/mutants were app

60 hemistry experiments using a polyclonal anti-IGFBP-2 antibody were performed to confirm IGFBP-2 prote

61 Levels of IGFBP-2 are elevated frequently in human tumors: its abi

62 rther support the development of full-length IGFBP-2 as a cancer therapeutic.

63 found that IGF-II enhanced by over threefold IGFBP-2 binding to extracellular matrix produced by huma

64 BP-2 with a specific antibody, or preventing IGFBP-2 binding to integrins, restored the induction of

65 These cells predominantly produce IGFBP-2: blocking IGFBP-2 with a specific antibody, or p

66 IGFBP-2 bound RPTPbeta, which led to its dimerization an

67 cells stably transfected with the zebrafish IGFBP-2 cDNA secreted a 31-kDa protein, which bound to I

68 cellular matrix-rich environment, the IGF-II/IGFBP-2 complex was as effective as IGF-II alone in stim

69 e the prospective association of circulating IGFBP-2 concentrations and of differential methylation i

70 Immunohistochemistry studies for IGFBP-2 confirmed the expression pattern found by in sit

71 Plasma IGFBP-2 content was significantly reduced in prenatal T-

72 .027, = 0.014, = 0.048, respectively), while IGFBP-2, CTRC and RAGE were associated with lower ASM (P

73 ratio of IGF-I to IGFBP-3 increased, whereas IGFBP-2 decreased throughout pregnancy.

74 20-fold decrease in IGF-1 binding affinity (IGFBP-2 EC50 = 0.35 nM and IGFBP-2(1-248) = 7 nM).

75 IGFBP-2 enhanced IGF-I-stimulated VSMC migration and pro

76 ively, these studies establish that PTEN and IGFBP-2 expression are inversely correlated in human bra

77 his study was conducted to determine whether IGFBP-2 expression in human RPE cells from the macula an

78 is a topographical and age-related change in IGFBP-2 expression in RPE cells from human donor eyes.

79 er investigate this link, we determined that IGFBP-2 expression is negatively regulated by PTEN and p

80 To further test their contributions to IGFBP-2 function, the single tryptophan in human IGFBP-2

81 As that encode transcription factors for the IGFBP-2 gene also were induced in some ST tissues.

82 lated IGFBP-2 mRNA abundance and transfected IGFBP-2 gene promoter activity in human (Hec-1-A) uterin

83 tions and of differential methylation in the IGFBP-2 gene with type 2 diabetes risk.

84 nsulin-like growth factor binding protein-2 (IGFBP-2) gene.

85 is unique to IGFBP-1 as the closely related IGFBP-2 had no effect, a finding consistent with its ina

86 We conclude that the HBD peptide of IGFBP-2 has anabolic activity by activating IGF-I/Akt an

87 t IGF-I and its binding proteins IGFBP-1 and IGFBP-2 have potentially beneficial effects on diabetes

88 nsulin-like growth factor binding protein 2 (IGFBP-2); histone H2B.1; basement membrane heparan sulfa

89 complexes with several IGF-binding proteins (IGFBP-2, IGFBP-3, and IGFBP-5).

90 P-A, PAPP-A2, total and free IGF-1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-5 and STC2 were measured by ELIS

91 IGF-I and several forms of IGFBPs, including IGFBP-2, IGFBP-4, and IGFBP-5.

92 In sum, the IGF axis, IGFBP-2 in particular, may be implicated in the pathogen

93 broblasts, implicating a functional role for IGFBP-2 in PTEN signaling.

94 Increased IGFBP-2 in these cultures was associated with a 1.41-fol

95 h increased levels of IGF-binding protein-2 (IGFBP-2) in the uterus and ovary, and a reduction in IGF

96 In the current study, we postulated that IGFBP-2 increased bone mass partly through the activity

97 These results indicate that zebrafish IGFBP-2 is a negative growth regulator acting downstream

98 For example, IGFBP-2 is expressed in the inner circular layer shortly

99 data support a growing body of evidence that IGFBP-2 is not just a transport protein but rather that

100 nsulin-like growth factor-binding protein 2 (IGFBP-2) is a member of a family of six highly conserved

101 nsulin-like growth factor binding protein 2 (IGFBP-2) is frequently overexpressed in the highly invas

102 IGFBP-2 knockdown led to decreased PTEN tyrosine phospho

103 IM-1), HPGDS, ICAM-1 (CD54), ICAM-2 (CD102), IGFBP-2, LCN2, NCAM-1 (CD56), SELE (E-Selectin), SELL (L

104 ic GH (12.32 vs. 50.97 pg/mL, p < 0.001) and IGFBP-2 levels (51.86 vs. 68.81 pg/mL, p < 0.001) were e

105 calized prostate cancer, preoperative plasma IGFBP-2 levels are inversely associated with biologicall

106 ain and prostate cancers and implicate serum IGFBP-2 levels as a potential serum biomarker of PTEN st

107 f particular interest because elevated serum IGFBP-2 levels have been reported in patients with prost

108 Elevation of plasma IGFBP-2 levels in prostate cancer patients apparently is

109 was fivefold lower among women with baseline IGFBP-2 levels in the top versus bottom quintile (odds r

110 dy suggests that improved circulating GH and IGFBP-2 levels may mediate the beneficial effects of gas

111 IGFBP-2 levels rise during rapid neonatal growth and at

112 IGFBP-2 levels were elevated in prostate cancer patients

113 GH, IGF-1 and IGFBP-2 levels were evaluated by ELISA at baseline and 6

114 prostatectomy patients, preoperative plasma IGFBP-2 levels were lower in patients with advanced dise

115 Serum IGF-I, IGFBP-1, and IGFBP-2 levels were measured by radioimmunoassay, and IG

116 relative to L/L-TRAMP mice (P < 0.001), but IGFBP-2 levels were not different.

117 In contrast, higher IGFBP-2 levels were related to a substantially lower ris

118 Thus, IGFBP-2 may facilitate the targeting of IGFs, and in par

119 nsulin-like growth factor binding protein 2 (IGFBP-2) may protect against type 2 diabetes, but popula

120 Analysis of aortas obtained from IGFBP-2(-/-) mice showed that RPTPbeta was activated, an

121 NA abundance, whereas, putrescine stimulated IGFBP-2 mRNA abundance and transfected IGFBP-2 gene prom

122 IGFBP-2 mRNA expression was detected in the ganglion cel

123 In 16 of 17 eyes, IGFBP-2 mRNA expression was detected in the RPE.

124 The total numbers of RPE cells and IGFBP-2 mRNA expression-positive RPE cells were counted

125 In adult zebrafish, IGFBP-2 mRNA levels were greatly reduced by growth hormo

126 in insulin growth factor-binding protein 2 (IGFBP-2) mRNA.

127 insulinlike growth factor-binding protein 2 (IGFBP-2), nerve growth factor (b-NGF), platelet-derived

128 xia did not affect protein or mRNA levels of IGFBP-2 or -4.

129 r, these results suggest that, once bound to IGFBP-2 or IGFBP-3, the biotin moieties of N(alpha)(Gly1

130 pression of PAPP-A led to degradation of the IGFBP-2 produced by C2C12 myoblasts and increased free I

131 unt of free IGFs via specific degradation of IGFBP-2 produced by myoblasts.

132 Compared with untreated controls, IGFBP-2 production was significantly increased (P < 0.00

133 chromatin, they abolish menin binding to the IGFBP-2 promoter in vivo.

134 i-IGFBP-2 antibody were performed to confirm IGFBP-2 protein expression.

135 domain on IGF-1 contacts the distal third of IGFBP-2, providing evidence that the IGF-1-binding domai

136 nsulin-like growth factor-binding protein-2 (IGFBP-2) resulted in an increase in synthesis in both th

137 ypsin-digested abG(1)IGF-1.recombinant human IGFBP-2 (rhIGFBP-2) complex indicated photoincorporation

138 Recombinant human IGFBP-2 significantly attenuated IGF-1-stimulated MCF-7

139 zebrafish and mammalian cells, the zebrafish IGFBP-2 significantly inhibited IGF-I-stimulated cell pr

140 insulin-like growth factor binding protein (IGFBP-2) that comprises 290 amino acid residues, a phosp

141 roximately 50-kD complex in association with IGFBP-2; this complex can cross the capillary barrier an

142 n 45 (IIp45), whose protein product bound to IGFBP-2 through the thyroglobulin-RGD region of the C te

143 rn blot analyses revealed that the zebrafish IGFBP-2 transcript is a 1.8-kb band expressed in many em

144 P-2 function, the single tryptophan in human IGFBP-2, Trp-248, was selectively cleaved with 2-(2'nitr

145 In Boyd Orr, a one SD increase in IGFBP-2 was associated with 2.6% slower get-up and go ti

146 Up-regulation of IGFBP-2 was confirmed at the protein level by Western bl

147 IGFBP-2 was present in all samples, IGFBP-4 in sporadic

148 Of the IGFBPs, an increase in IGFBP-2 was unique to these patients and was not found i

149 ontrast, serum levels and mRNA expression of IGFBP-2 were increased 1.6-fold (P = .003) and twofold (

150 GD-IGFBP-1, des(1-3)IGF-I/IGFBP-1, and IGF-I/IGFBP-2 were ineffective.

151 GH, IGF-1, and IGFBP-2 were not correlated with insulin or lipid parame

152 oses of IGF-II due to induction of PTEN, but IGFBP-2, when free from IGF-II can suppress PTEN.

153 of insulin growth factor-binding protein-2 (IGFBP-2), which is secreted thereby promoting angiogenes

154 ells predominantly produce IGFBP-2: blocking IGFBP-2 with a specific antibody, or preventing IGFBP-2