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1                                              IPTG treatment caused parallel increases in the levels o
2                                              IPTG-induced C/EBPalpha caused inhibition of cell prolif
3                                              IPTG-induced dCas9 expression enabled validation of esse
4                                              IPTG-induced pilin rescued S motility of the DeltapilA s
5  very low inducer concentrations (3-10microM IPTG) and reached levels significantly higher than in st
6                                Additionally, IPTG-controlled Cas9 expression combined with a constitu
7 ive in the Lon and OmpT proteases and allows IPTG-inducible expression of recombinant proteins from t
8                                           An IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible
9  mevalonate pathway genes is regulated by an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible
10 of BosR in B. burgdorferi, we constructed an IPTG (isopropyl-beta-d-thiogalactopyranoside)-regulated
11         In this study we have constructed an IPTG-inducible GFP expression system in M. tuberculosis
12 ichia coli RNA metabolism, we constructed an IPTG-inducible pcnB [poly(A) polymerase I, PAP I] contai
13  corresponds closely to that observed for an IPTG-inducible A14 recombinant whose construction and ch
14 n of the 14 genes in the bfp cluster from an IPTG-inducible promoter, in the absence of pJPN14, is su
15  that conditionally expressed FbpABC from an IPTG-inducible promoter.
16 more, induced expression of the system in an IPTG-inducible plasmid in a reference strain of K. pneum
17 tants and analyzed their activities using an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible
18                 Additionally, assays with an IPTG-regulatable bb0795 mutant revealed that BB0795 is r
19 t was fully reversible by the lactose analog IPTG.
20                Addition of a lactose analog, IPTG, to the swimming water of the axolotl is sufficient
21 lets, we study the diffusion of both AHL and IPTG from inducer-filled reservoirs into bacteria-contai
22   Engineered bacteria that integrate AHL and IPTG signals with a synthetic AND gate gene circuit are
23 ilizes but does not eliminate the loops, and IPTG does not redistribute loops among high-FRET topolog
24     When cells were treated with both Tc and IPTG, full induction of cat was reached in 24 h and main
25 her cell functions originally designed to be IPTG-inducible.
26 1 phosphatase is regulated experimentally by IPTG (isopropyl-beta-D-thiogalactopyranoside) (35).
27 sense or pUC19 sequences could be induced by IPTG were also constructed.
28 d two-fold when transcription was induced by IPTG, but had no detectable effect on protein yield when
29 sformation competence was also influenced by IPTG levels in the growth medium.
30 were rescued from those plasmids that caused IPTG-dependent cell death.
31                 In contrast, in these cells, IPTG-dependent induction of p14ARF, which sequesters MDM
32                        Also, the conditional IPTG inducible expression of wild type Sgk, but not of t
33                                D149 contacts IPTG directly, and variations at this site provide the o
34 cted from the immunoprecipitate demonstrated IPTG (isopropylthiogalactoside)-sensitive binding of GFP
35 lot analysis, we demonstrated dose-dependent IPTG induction of LLO during growth in broth culture.
36 ession dynamics of 8269 rationally designed, IPTG-inducible promoters that collectively explore the i
37 tested: five time points and three different IPTG and 3-AT concentrations.
38                           Fusions exhibiting IPTG-responsive beta-galactosidase activity were cloned
39                                    Following IPTG (isopropyl-beta-D-thiogalactopyranoside) induction,
40 that endogenous p21 mRNA increased following IPTG removal, which may be responsible for the continued
41 hey contained increased Tat levels following IPTG induction.
42  cell paste of R.XmnI was obtained following IPTG induction in a suitable E. coli host.
43 lative gene expression changes when RNA from IPTG-induced cells was labeled and compared to RNA from
44 lta virB operon mutant expressing virB7 from IPTG-inducible Pklac and virB9 from an AS-inducible Pvir
45 M isopropyl-1-thio-beta-D-galactopyranoside (IPTG) induced the expression of Ha-Ras(Val12), closely f
46 nder isopropylthio-beta-D-galactopyranoside (IPTG)-inducible control.
47 h isopropyl-1-thio-beta-D-galactopyranoside (IPTG).
48  or isopropyl-beta-D-thio-galactopyranoside (IPTG) can be utilized for the implementation of an artif
49 inhibitor isopropyl thio-beta-D-galactoside (IPTG) requires the mutant enzyme to adopt its less favor
50 dition of isopropyl-beta-D-thio-galactoside (IPTG) destabilizes but does not eliminate the loops, and
51 tion of ERK1/2 and the induction of COX-2 in IPTG-induced Rat-1:iRas cells.
52    No free-citrate transport was observed in IPTG (isopropyl-beta-d-thiogalactopyranoside)-induced or
53 lls expressing a non-mutated Fd (UMHC) or in IPTG-treated non-transduced V79 cells.
54 d retroviral vector, MSCV-In(S), resulted in IPTG-responsive oncogene expression and cell growth.
55 ia that process different stimuli, including IPTG, aTc and arsenic.
56                           For L148F, inducer IPTG binding affinity is strengthened, whereas O(1) oper
57  to increasing concentrations of the inducer IPTG (isopropyl-beta-D-thiogalactopyranoside) and were a
58 ur when LacI binds either DNA or the inducer IPTG, or both.
59 biomaterials to present the genetic inducer, IPTG, with different modes of activating genetic circuit
60 recombinant virus in which F18 expression is IPTG (isopropyl-beta-d-thiogalactopyranoside) dependent,
61  the control of the Pspac promoter, which is IPTG inducible.
62 her involves isopropylthiogalactopyranoside (IPTG)-induced synthesis of sigma 32 at 30 degrees C from
63    This promoter, LTRo, was shown to mediate IPTG-inducible cat expression in rat cells expressing th
64 ion optimization at 20 degrees C with 0.1 mM IPTG.
65 on of expression at 16 degrees C with 10 muM IPTG yields 80% monomeric cyclic protein.
66 ollowing infection of mice in the absence of IPTG or d-alanine, the bacterium survived in vivo for le
67 croscopy of cells infected in the absence of IPTG revealed normal-appearing crescents and immature vi
68  of knockout mutants, even in the absence of IPTG.
69 nslocate through the block until addition of IPTG induces repressor dissociation.
70 scape into the cytosol following addition of IPTG to the cell culture medium, thus yielding the abili
71  Rec- and Rec+ by the removal or addition of IPTG.
72 MOPS) medium containing different amounts of IPTG.
73        In addition, systemic applications of IPTG were used to induce genetic circuits in cells encap
74                               The binding of IPTG to native (tetrameric) LacI-oDNA complexes also app
75 directly correlate with the concentration of IPTG (isopropyl-beta-D-thiogalactopyranoside) in the gro
76 lacY mutant and varying the concentration of IPTG.
77 ystem II and system I, both under control of IPTG, we have begun to study the capabilities and charac
78 led in the murine retina by oral delivery of IPTG over three induction-repression cycles.
79 expression increased smoothly as the dose of IPTG added to the culture was increased.
80 y inducible trc promoter, the elimination of IPTG, the inclusion of the tetracycline resistance gene,
81 eloped and demonstrated the functionality of IPTG-inducible heterologous protein expression in Leptos
82 nd pilin protein in response to the level of IPTG in the growth medium.
83                  An increase in the level of IPTG induced a sphere-to-short rod transition that re-es
84 ac-fnr delta resDE mutant in the presence of IPTG indicated that resDE has an additional role in B. s
85    The particles prepared in the presence of IPTG were more infectious than those prepared in its abs
86 te starvation conditions, in the presence of IPTG, all strains containing mutated phoR genes showed a
87 ict dose-dependent growth in the presence of IPTG.
88                                   Removal of IPTG reverted the cell to full G6PD activity.
89 In the absence of an inducer (allolactose or IPTG) this strain, DH1 lackan , cannot grow on kanamycin
90 s concentrations of tetracycline (Tc) and/or IPTG.
91           Hence, treatment with either Tc or IPTG led to a tenfold increase in CAT activity.
92 ion could be alleviated by exposure to Tc or IPTG, which inhibited the binding activities of TTA and
93 plasmid vector with each cDNA having its own IPTG-inducible promoter.
94 sed a fusion of rpoD to the LacI-repressible IPTG-inducible promoter, Pspac, to vary the levels of si
95 , even after removal of the inducing signal (IPTG).
96 d induced by the non-metabolizable substrate IPTG.
97     Metabolic labelling of newly synthesized IPTG-induced proteins during/absence of proteasomal inhi
98 oes not reduce FRET further, suggesting that IPTG stabilizes extended or other low-FRET loops.
99 e consistency in the phenotypes seen for the IPTG- and TET-inducible recombinants confirms the effica
100         The protein was overexpressed in the IPTG-inducible vector pET14b-TnsD in E. coli BL21(DE3)-R
101 ription, fnr was placed under control of the IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible
102 spx gene was placed under the control of the IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible
103 ilis strain carrying a fusion of rodA to the IPTG-inducible Pspac promoter is inducer dependent.
104  with isopropyl B-D-1-thiogalactopyranoside (IPTG) has been used to regulate gene expression in Esche
105 se isopropyl beta-d-1-thiogalactopyranoside (IPTG) induction.
106 e, Isopropyl beta-D-1-thiogalactopyranoside (IPTG) that has no off-target effects in mammals.
107 of isopropyl beta-D-1-thiogalactopyranoside (IPTG)-inducible mutant T7 promoters of varied strength a
108 r, isopropyl beta-D-1-thiogalactopyranoside (IPTG).
109 er isopropyl beta-D-1-thiogalactopyranoside (IPTG, which inhibits the interaction between the lac rep
110 t on isopropyl-beta-D-thiogalactopyranoside (IPTG) for expression has been inserted into the genome.
111 sing isopropyl beta-D-thiogalactopyranoside (IPTG) in a bacterial strain that harbors the lacIq gene,
112 rief isopropyl-beta-D-thiogalactopyranoside (IPTG) treatment elevated lacZ, lacY, and lacA transcript
113 with isopropyl-beta-D-thiogalactopyranoside (IPTG) were constructed.
114      Isopropyl-beta-D-thiogalactopyranoside (IPTG), an inhibitor of the lac repressor, can prevent de
115 with isopropyl beta-d-thiogalactopyranoside (IPTG), we were able to detect 10 CFU.mL(-1) in drinking
116 f an isopropyl-beta-D-thiogalactopyranoside (IPTG)-dependent promoter.
117 d an isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible M. mycoides subsp. mycoides SC biotype p
118 from isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible Plac and other virB genes from acetosyri
119 ated isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible promoter present on a multicopy plasmid.
120   An isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible promoter was constructed in Myxococcus x
121 f an isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible promoter.
122 from isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible promoters for the purpose of continuousl
123 from isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible promoters.
124 m an isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible yirB construct.
125 g an isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated promoter upstream of the ribosomal bindi
126 t on isopropyl-beta-D-thiogalactopyranoside (IPTG).
127 with isopropyl-beta-D-thiogalactopyranoside (IPTG).
128 log, isopropyl-beta-D-thiogalactopyranoside (IPTG).
129 d by isopropyl-beta-D-thiogalactopyranoside (IPTG).
130 ucer isopropyl-beta-D-thiogalactopyranoside (IPTG).
131 with isopropyl-beta-D-thiogalactopyranoside (IPTG).
132  isopropyl-beta-delta-thiogalactopyranoside (IPTG) inducible promoter, and transfected and then expre
133  inducer isopropyl-beta-D-1-thiogalactoside (IPTG) and the lac repressor complexed with a 21-base pai
134  the inducer isopropyl-beta-thiogalactoside (IPTG), repression was relieved.
135 he inducer isopropyl-beta-D-thiogalactoside (IPTG), iA6 replicated normally, and membrane proteins of
136 ddition to isopropyl-beta-d-thiogalactoside (IPTG), to the growth medium of the E. coli expression ho
137 with the data for isopropyl thiogalactoside (IPTG), but somewhat discrepant with respect to the data
138 ression by 0.5 mM isopropyl thiogalactoside (IPTG).
139 in production, reaching yields comparable to IPTG induction but with easier tunability of expression.
140 ion by 24% and 27% respectively, compared to IPTG induction, in light-controlled fermentations scalab
141 erepression of the transposase gene prior to IPTG induction.
142 macrophages, M. tuberculosis that respond to IPTG with expression of GFP fluorescence, and hence are
143 osis that fail to express GFP in response to IPTG, and hence are metabolically inactive, reside withi
144 otype pyruvate dehydrogenase region, and two IPTG-independent polypeptides, of 29 kDa and 57 kDa, wer
145 nges occur in the R3 dimer-oDNA complex upon IPTG binding, indicative of small adjustments in the ori
146 e cell line J774 was strictly dependent upon IPTG.
147 nt of view, since it is not necessary to use IPTG.
148 ted genes independently of the commonly used IPTG and arabinose strategies.
149 usion protein was expressed in E. coli using IPTG induction and purified using FMSN-Ni-NTA.
150 sform up to 5 mM of phenol and tyrosol using IPTG (isopropyl-beta-D-thiogalactopyranoside) as inducer
151 LAC, to control gene expression from various IPTG-inducible promoters using only blue light.
152 cell spread of iLLO bacteria in L2 cells was IPTG dose dependent.
153 l induction was reached after 192 h, whereas IPTG addition led to full induction within 24 h.
154 O(1) binding is increased for S151P, whereas IPTG binding is decreased.
155 iskly induced by treatment of the cells with IPTG (EC50 13.7 microM).
156 te rhamnose-induced expression compared with IPTG-induced expression from lacp.
157 ne circuits were activated in materials with IPTG embedded within the scaffold walls or chemically li
158 ither native promoter or Pspac promoter with IPTG induction, resulted in a similar level of alkaline
159                     Unless supplemented with IPTG (which induces expression of dapD) or DAP, these ce
160                      Combined treatment with IPTG and TGF-beta1 resulted in a 20-50-fold increase in
161                      Combined treatment with IPTG and TGF-beta1 synergistically increased the lucifer
162                               Treatment with IPTG resulted in high level induction of the lacZYA and
163  biotransformation rate was attained without IPTG, confirming that this fragment encodes for a phenol

 
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