ライフサイエンスコーパス: ITR

1 ITR promoter activity was reduced by altering the miS1 e

2 ITRs found across species tend to be more divergent in e

3 ITRs stabilized in absence of SbcC, which is part of the

4 ITRs with conserved expression and ancient retained dupl

5 AAV2 Rep78 cleaved only a type 2 ITR DNA sequence, and AAV5 Rep78 cleaved only a type 5 p

6 onsisting of adeno-associated virus types 2 (ITRs and Rep) and 5 (Cap) were evaluated for muscle-dire

7 Among 43,301 ITRs across the four species, 34,460 (80%) are species-s

8 ector characterized by an AAV-2 and an AAV-5 ITR at opposite ends of the viral genome.

9 ITR sequences contained within hybrid AV2:5 ITR vectors could direct intermolecular recombination in

10 Hybrid AV2:5 ITR viruses had a significantly lower capacity to form c

11 fected cells than homologous AV2:2 and AV5:5 ITR vectors despite their similar capacity to express an

12 = 4 microg/ml for most isolates (POS, 97.6%; ITR, 96.3%; VOR, 95.9%; RAV, 93.5%).

13 ng rep68/78 outside the rep(-) vector 3' AAV ITR sequence.

14 gration into the host genome through the AAV ITR(s).

15 is found only in the hairpin form of the AAV ITR, causes a three- to eightfold reduction in the abili

16 This indicates that rescue of the AAV ITR-flanked transgene cassette from Ad.AAV-LCR-GFP is no

17 es contain only the transgene flanked by AAV ITRs, Ad packaging signals, and Ad ITRs.

18 were present; a second plasmid contained AAV ITRs flanking the reporter gene (integration cassette),

19 The presence of two intact AAV ITRs was crucial for the production of hybrid vectors an

20 e findings indicate that the presence of AAV ITRs and the Rep78 protein enhance the integration of DN

21 16-mer core sequences of the RRSs in the AAV ITRs and AAVS1 separately as query sequences and identif

22 We hypothesized that the AAV ITRs present within the hybrid vector genome could media

23 nd-specific endonuclease cuts within the AAV ITRs, and display nucleoside triphosphate-dependent heli

24 erting a transgene cassette flanked with AAV ITRs into an HSV-1 amplicon backbone, and a rep(+) HSV/A

25 olved in illegitimate recombination with AAV ITRs, deletions of which significantly reduced the exten

26 nt in the packaging of a genome with an AAV2 ITR.

27 Comparison of the TRSs in the AAV2 ITR, the AAV5 ITR, and the AAV chromosome 19 integration

28 imilar affinities for either an AAV2 or AAV5 ITR; however, comparison of terminal resolution site (TR

29 Mapping of the AAV5 ITR TRS identified a distinct cleavage site (AGTG TGGC)

30 arison of the TRSs in the AAV2 ITR, the AAV5 ITR, and the AAV chromosome 19 integration locus identif

31 s, transgene integration occurred via the Ad ITR.

32 ed by AAV ITRs, Ad packaging signals, and Ad ITRs.

33 enome can be created by breaks within the Ad ITRs, whose structure is apparently recognized by cellul

34 We also observed that adenovirus ITRs in the helper plasmid were involved in illegitimate

35 e and helper plasmids lacking the adenovirus ITRs led to complete elimination of replication-competen

36 but sustained elevation of PP and CVPP after ITR stimulation.

37 Using antibodies against ITR-1 we detected a protein of 220 kDa in C. elegans mem

38 , infection of hESCs induced gammaH2AX in an ITR-independent manner, however, canonical activation of

39 The data herein reveal an ITR-dependent AAV vector transduction restriction specif

40 Ectopic expression of ZF5 leads to an ITR-dependent repression of the autologous p5 promoter a

41 insect cell lines stably transfected with an ITR-flanked transgene exceeded 60 mg per 5 x 10(9) Sf9 c

42 n two myosin proteins, UNC-54 and MYO-1, and ITR-1 were identified in a yeast two-hybrid screen and s

43 those between VOR and RAV MICs (r = 0.7) and ITR and POS MICs (r = 0.4).

44 s than or equal to the ECV) for AMB, FC, and ITR, respectively, were 2 (99.8)/2 (99.2), 0.5 (94.2)/1

45 irrel monkeys with ablations of ITC; ITI and ITR (group ITRd); or ITI, ITR, and more ventral cortex,

46 ly, for A. flavus, only VOR and RAV MICs and ITR and POS MICs demonstrated statistically significant

47 were strongest between VOR and RAV MICs and ITR and POS MICs.

48 spartate type glutamate receptor subunit and ITR-1 inositol 1,4,5-triphosphate receptor.

49 ve the highest homology with the rep ORF and ITRs of AAV serotype 5 (AAV-5) (89 and 96%, respectively

50 Whereas the Rep proteins and ITRs of other serotypes are interchangeable and can be u

51 strated that, in the presence of Rep78, both ITR substrates targeted to ch-19 with similar frequency.

52 e studies demonstrate that this novel 165-bp ITR substrate is sufficient in cis for the AAV life cycl

53 ssociated with anxiety, depression, burnout, ITR, and ITL.

54 rkload, and single-item measures of burnout, ITR, and ITL.

55 epithelial cell sheets can be controlled by ITRs and suggest that such regulation may be a widesprea

56 aced with a transgene of interest flanked by ITRs and subsequently packaged within an AAV capsid made

57 r the human factor IX (hfIX) gene flanked by ITRs from AAV genotypes 1 through 6.

58 e integration cassette alone (GFP flanked by ITRs) produced stable fluorescent colonies at a frequenc

59 ombination was enhanced, whereas open-closed ITR recombination was reduced in the presence of HSV-1.

60 We never detected a complete ITR at a junction.

61 To examine whether the divergent ITR sequences contained within hybrid AV2:5 ITR vectors

62 Each ITR also contains a stretch of 20 nucleotides, designate

63 The terminal 125 nucleotides of each ITR form palindromic hairpin (HP) structures that serve

64 replacement of only one D sequence in either ITR restores each of these functions, but DNA strands of

65 In the absence of either factor, ITR hairpin opening was impaired, resulting in accumulat

66 pergillus isolates (from 88% of isolates for ITR to 98% of isolates for VOR and POS), MICs of each tr

67 Resulting full ITR transgene plasmids improved rAAV yield and purity in

68 l cortex, including perirhinal cortex (group ITR+), with visual learning in unoperated controls.

69 ys with ablations of ITC; ITI and ITR (group ITRd); or ITI, ITR, and more ventral cortex, including p

70 the capsid serotype) compared to homologous ITR vectors.

71 The use of hybrid ITR AAV vector genomes provides new strategies to manipu

72 rom this comparison demonstrated that hybrid ITR dual-vector sets had a significantly enhanced trans-

73 viral genome structures suggest that hybrid ITR vectors provide more efficient directional recombina

74 ail-to-head fashion, we generated two hybrid ITR trans-splicing vectors (AV5:2LacZdonor and AV2:5LacZ

75 To this end, we generated a novel AAV hybrid-ITR vector characterized by an AAV-2 and an AAV-5 ITR at

76 To assess if ITRs are functional (under selection), machine learning

77 s revealed various rearrangements, including ITR deletions and amplifications of the vector and cellu

78 evaluated the involvement of the individual ITRs as well as their components in the selective viral

79 tions in different regions of the individual ITRs were constructed and examined for their potential t

80 ns of ITC; ITI and ITR (group ITRd); or ITI, ITR, and more ventral cortex, including perirhinal corte

81 thod for amphotericin B (AMB), itraconazole (ITR), voriconazole (VOR), posaconazole (POS), and ravuco

82 B (AMB), flucytosine (FC) and itraconazole (ITR) for eight Candida spp. (30,221 strains) using isola

83 ntimicrobial agents, including itraconazole (ITR), were prescribed during hospitalization and at the

84 8-A broth dilution method with itraconazole (ITR), posaconazole (POS), ravuconazole (RAV), and vorico

85 ed from the plasmid that lacked the AAV left ITR, suggesting the existence of an additional putative

86 hat the hairpin form, rather than the linear ITR, is the more frequent integration substrate.

87 anism by which the virus is able to maintain ITR sequence continuity and persist in the absence of ho

88 This retrospective matched ITR analysis revealed no difference in rejection and in

89 Molecular characterization of the mutant ITR integrants confirmed the presence of the trs mutatio

90 rovide direct evidence for the importance of ITR sequences in directing intermolecular and intramolec

91 ns for four weeks prior to the initiation of ITR.

92 was that B-AAV generated a greater level of ITR-initiated RNAs than did A-AAV or AAV5.

93 CCS was associated with 91% greater odds of ITR (OR, 1.91; 95% CI, 1.76 to 2.08; P < .001) and 28% g

94 Stimulation of ITR neurons produced respiratory effects, but cardiovasc

95 Ephrin/VAB-1 signaling acts upstream of ITR-1 to inhibit meiotic resumption, while NMR-1 prevent

96 ed both numeric and positional variations of ITRs, which were similar to the variations in tetraploid

97 nd (vii) the AAV genomes containing only one ITR structure can be packaged into biologically active v

98 as +/-2400 copies of a derived 80 bp paired ITR structure and +/-4600 copies of solo ITRs.

99 ent assignment and to estimate a preliminary ITR in a 70% training sample and to evaluate the ITR in

100 The InsP3R protein (ITR-1) is approximately 42 % identical with known InsP3R

101 known as intimal thickness-related receptor (ITR).

102 ingle inositol 1,4,5-trisphosphate receptor (ITR) in C. elegans.

103 s between CCS and burnout, intent to reduce (ITR) hours, and intent to leave (ITL) are not known.

104 own that the pontine intertrigeminal region (ITR) plays an important role in respiratory regulation,

105 nvolving the pontine intertrigeminal region (ITR), has a physiological role in mediating or modulatin

106 servation of intergenic transcribed regions (ITRs) in four Poaceae species.

107 The Intestinal Transplant Registry (ITR) was created in 1985 by the Intestinal Transplant As

108 he role of the AAV inverted terminal repeat (ITR) cis-acting sequences in targeted integration an AAV

109 t utilize a mutant inverted terminal repeat (ITR) for vector production.

110 d Artemis open AAV inverted terminal repeat (ITR) hairpin loops in a tissue-dependent manner.

111 site (RBS) of AAV inverted terminal repeat (ITR) in males compared with females.

112 formed by the AAV inverted terminal repeat (ITR) origins of replication, make site- and strand-speci

113 re was substantial inverted terminal repeat (ITR) promoter activity that correlated with neuropatholo

114 rticular, open-end inverted terminal repeat (ITR) recombination was enhanced, whereas open-closed ITR

115 d mutations in the inverted terminal repeat (ITR) regions due to their inherent instability, which is

116 Rep) in trans, and inverted terminal repeat (ITR) sequences in cis.

117 echanisms by which inverted terminal repeat (ITR) sequences mediate intermolecular recombination of A

118 ts were within the inverted terminal repeat (ITR) sequences, which contain RBSs.

119 ence of a double-D inverted terminal repeat (ITR) structure, which implied a mechanism by which the v

120 copies of a 145-bp inverted terminal repeat (ITR) that flank the AAV genome.

121 te within the AAV5 inverted terminal repeat (ITR), and mapping of the AAV5 intron revealed that it is

122 Within the inverted terminal repeat (ITR), several base changes were identified with respect

123 bution of the interstitial telomere repeats (ITRs).

124 te flanked by AAV inverted terminal repeats (ITRs) (Ad.AAV-LCR-GFP), transduced cells were cloned and

125 rticular focus on inverted terminal repeats (ITRs) and AAV capsid-genome interactions, which play a c

126 sensus has 31 bp imperfect terminal repeats (ITRs) and encodes a 351 amino acid (aa) mariner transpos

127 AAV fragments and inverted terminal repeats (ITRs) and failed to detect full-length AAV.

128 ically to the AAV inverted terminal repeats (ITRs) and possess ATPase, helicase, and strand-specific/

129 deletions of the inverted terminal repeats (ITRs) and their flanking sequences.

130 The inverted terminal repeats (ITRs) are also unique compared to those of the other AAV

131 by using rep and inverted terminal repeats (ITRs) from AAV2 and were compared with similarly constru

132 me (ORF) and both inverted terminal repeats (ITRs) have the highest homology with the rep ORF and ITR

133 passing two viral inverted terminal repeats (ITRs) in a head-to-tail orientation, which confers a 10-

134 both ends of the inverted terminal repeats (ITRs) in the adeno-associated virus serotype 2 (AAV2) ge

135 n between (i) AAV inverted terminal repeats (ITRs) in the recombinant plasmid and (ii) AAV sequences

136 iated virus (AAV) inverted terminal repeats (ITRs) induce p53-dependent apoptosis in human embryonic

137 oporation of AAV2 inverted terminal repeats (ITRs) is sufficient to induce cell death.

138 ) genome contains inverted terminal repeats (ITRs) of 145 nucleotides.

139 repeats, such as inverted terminal repeats (ITRs) of adeno-associated virus (AAV).

140 he right and left inverted terminal repeats (ITRs) of the transposon.

141 These inverted terminal repeats (ITRs) play an important role in AAV DNA replication and

142 has 30 bp perfect inverted terminal repeats (ITRs), and encodes a 343 amino acid (aa) mariner transpo

143 (RRSs) in the AAV inverted terminal repeats (ITRs), the AAV p5 promoter, and the preferred AAV integr

144 taining the viral inverted terminal repeats (ITRs), transgene expression cassette (human alpha 1-anti

145 played by the AAV inverted terminal repeats (ITRs), which consist of a palindromic hairpin (HP) struc

146 8/68 proteins and inverted terminal repeats (ITRs).

147 involving the AAV inverted terminal repeats (ITRs).

148 rames between two inverted terminal repeats (ITRs).

149 iated virus (AAV) inverted terminal repeats (ITRs).

150 rus (AAV) Rep and inverted terminal repeats (ITRs).

151 promoter, and the inverted terminal repeats (ITRs).

152 kaging sequences (inverted terminal repeats [ITRs]) derived from AAV genotypes other than 2.

153 Residual ITRs at junctions never contained more than one RBS, sug

154 nknown if an interfacial thermal resistance (ITR) occurs in matter with high-energy-density where fre

155 ying strains and culture conditions revealed ITR instability caused by a slipped misalignment mechani

156 on the models, 584 (8%) and 4391 (61%) rice ITRs are classified as likely functional and nonfunction

157 mes that lacked either the left or the right ITR sequence and one of the Rep-binding sites (RBSs).

158 udal (ITC), intermediate (ITI), and rostral (ITR) subdivisions, possibly homologous to TEO, posterior

159 to develop an individualized treatment rule (ITR) to optimize major depressive disorder (MDD) treatme

160 athy induced by intensive treadmill running (ITR) in mice.

161 ning AAV inverted terminal repeat sequences (ITRs) flanking a reporter gene cassette inserted into th

162 est with inverted terminal repeat sequences (ITRs) from adeno-associated virus.

163 activity in the liver that cleaved T-shaped ITR hairpin shoulders by making nicks at diametrically o

164 t to the AAV nicking site, flanking a single ITR.

165 red ITR structure and +/-4600 copies of solo ITRs.

166 ogous to H+[or Na+]/myo-inositol symporters (ITRs), not previously studied in plants.

167 To interrogate this phenomenon, a synthetic ITR (SynITR), harboring substitutions in putative p53 bi

168 On day 67 p.t., ITR was discontinued and liposomal amphotericin B (AMB)

169 n C phenocopy these defects, confirming that ITR function is important for proper epidermal migration

170 The ITR found that psychotherapy-only was the optimal treatm

171 d characterization of proteins that bind the ITR, the only viral genetic element retained in AAV2 vec

172 in a 70% training sample and to evaluate the ITR in the 30% test sample.

173 We explored the ITR impact on vagal reflex apnea elicited by intravenuou

174 randed circular episomes was similar for the ITR contexts, reporter expression was inhibited from tra

175 e conclude that a physiological role for the ITR in respiration is to attenuate vagally-induced refle

176 ume more vulnerable to perturbation from the ITR.

177 Microinjections of glutamate into the ITR produced apneas, while microinjections of kynurenic

178 e receptor antagonist), and red dye into the ITR, unilaterally and bilaterally.

179 njection of glutamate (10 mM, 30 L) into the ITR.

180 ature jump demonstrate the importance of the ITR for strongly heated systems with far-reaching implic

181 Neurons of the ITR have connections with the nucleus tractus solitarius

182 findings indicate a broad involvement of the ITR in autonomic regulation, including at least cardiova

183 ii) AAV vector infection, independent of the ITR sequence, induces activation of p53psi.

184 to eightfold reduction in the ability of the ITR to be used as a substrate for the Rep78 or MBP-Rep68

185 The 5' end of 3-base hairpin loops of the ITR was the primary target for DNA-PKcs- and Artemis-med

186 uld be needed to confirm the accuracy of the ITR.

187 Based on the ITR, 4156 ITx were performed between January 1987 and Ap

188 Our results suggest that the ITR strategy may be generally applicable to other system

189 CD spectra indicate that the ITR structure is largely B form DNA, although there is a

190 ely by competing with Rep for binding to the ITR.

191 nses to glutamate microinjections within the ITR in ketamine/xylazine anesthetized rats.

192 Within the ITR, the terminal resolution site and Rep binding sites

193 The ITRd monkeys had deficits on some pattern discrimination

194 The ITRd monkeys were significantly impaired on DNMS and som

195 sequences that lie outside the ITRs, and the ITRs also play a crucial role in excision of the provira

196 the contribution of the Rep proteins and the ITRs in the process of integration, we have compared the

197 hese differences in the Rep proteins and the ITRs result in a lack of cross-complementation between A

198 s also had a pair of loxP sites flanking the ITRs.

199 ge site (AGTG TGGC) which is absent from the ITRs of other AAV serotypes.

200 of an AAV5 rep/cap plasmid clone lacking the ITRs into 293 cells.

201 eplication and resolution, since each of the ITRs contains a terminal resolution site (trs) that is t

202 smids and the Rep-mediated resolution of the ITRs during AAV DNA replication, we constructed recombin

203 In all cases with the exception of the ITRs, the vector integrated intact.

204 h the AAV DNA sequences that lie outside the ITRs, and the ITRs also play a crucial role in excision

205 of the vector were mostly located within the ITRs, and cellular DNA sequences were recombined with th

206 gregate treatment costs of implementing this ITR would be negligible, as 16.1% fewer patients would b

207 icated probable resistance to AMB but not to ITR.

208 week topical ML pretreatment period prior to ITR induction, blood samples were collected for ELISA-ba

209 manipulation of the DNA damage response via ITR engineering.

210 No complete viral ITRs were directly observed.

211 sequence of impaired resolution of the viral ITRs.

212 enome was similar compared to wild type (wt) ITR, with a modest increase in vector titers.