ライフサイエンスコーパス: L chain

1 uced affinity compared with the other lambda L chains).

2 n RV144 V2 Ab but paired with a mouse lambda L chain.

3 myosin L chain kinase and regulatory myosin L chain.

4 ound B cells that coexpress kappa and lambda L chain.

5 expressing the 56R H chain with the lambdaX L chain.

6 ells that express lambdaX along with another L chain.

7 chain may be compensated by mutations on the L chain.

8 e junction of the variable (Vlambdax) editor L chain.

9 through the expression of a second innocuous L chain.

10 ferritin degraded at a faster rate than the L-chain.

11 romatin when they express certain endogenous L chains.

12 ambda and some even bear two different kappa L chains.

13 unit iron-storage complex assembled of H and L chains.

14 ene selection differences were also noted in L chains.

15 ave inactivated the H chain and secrete only L chains.

16 tive iron core nucleation site in vertebrate L chains.

17 ct HCs that will associate mainly with kappa L chains.

18 g protein specific for a V region site on Ig L chains.

19 he correct pairing of the two different free L chains.

20 ecific human mAb with naturally paired H and L chains.

21 gement, but not selection of Iglambda editor L chains.

22 pressed primarily in combination with editor L chains.

23 , <20% grouped cells expressing inconsistent L chains.

24 ed as monomeric polypeptides associated with L chains.

25 s been identified as neurofilament light (NF-L) chain.

26 custom-made siRNA for canine ferritin H- and L-chains.

27 g variable region of Ig heavy (H) and light (L) chains.

28 imately 11 kDa larger (30 kDa) than standard L-chain (19 kDa) purified from canine liver.

29 it2-S, increased neutrophil adhesion, myosin L chain 2 phosphorylation, and polarized actin formation

30 nction of the microtubule-associated protein L chain 3 (LC3) conjugation system in T lymphocytes rema

31 somes using a microtubule-associated protein L chain 3 (LC3) fusion protein effectively enhances and

32 L chain 3 (LC3)-associated phagocytosis is a process in

33 possible nucleation site is observed at the L chain 3-fold pore.

34 profile that included induction of ferritin L chain, a component of the inflammatory response.

35 as stimulation with anti-IgM or anti-Igkappa L chain Abs resulted in strong pCD79a and pPLCgamma2 sig

36 The overexpressed L-chain accumulated in the cytosol as predominantly homo

37 hrough expression of an additional Ig light (L) chain, accumulating gradually in lymphoid organs.

38 The extent to which paired L chains aids inference is unknown.

39 L chain allelic inclusion has been proposed as a B cell

40 B cells expressing two different Ig kappa L chains (allotype included) have been occasionally obse

41 However, exceptional editor L chains allow B cells to reach splenic compartments eve

42 The human H chains expressed with these L chains also have relatively high arginine (Arg) conten

43 hat expressed a transgene (Tg)-encoded kappa L chain and BM from TCR Tg mice in which the CD4 T cells

44 mutants that were impaired in assembly with L chain and in ability to be secreted from the cell.

45 old age, B2 pathways have limited surrogate L chain and increasingly generate new B cells with alter

46 iciency results in reduced usage of Iglambda L chains and a corresponding inhibition of Iglambda gene

47 d of two differently sized H chains bound to L chains and apparently often noncovalently associated w

48 were found to express both kappa and lambda L chains and display the high-affinity IgE Fc (Fcepsilon

49 tein sequences of mouse editors to all human L chains and found several human L chains similar to mou

50 Our findings can be extrapolated to human L chains and have implications for understanding a laten

51 The symmetrical arrangement of H and L chains and the disulfide crosslinks reflect adaptation

52 CX peak mAb isoform contained two unmodified L chains and two H chains terminating in glycine.

53 morphism of human and canine lens fiber cell L-chains and human H-chains.

54 e (Env) reactivity due to editing of the 2F5 L chain, and the majority of rescued B cells retained an

55 that there is no dependency on the surrogate L chain, and thus the authentic IgL proteins may be used

56 selecting VDJs that pair well with surrogate L chain, and whose maturation appears relatively indepen

57 Amplified heavy (H)- and light (L)-chain antibody segments were sequenced and used to id

58 sia ni, which reveals equal numbers of H and L chains arranged with tetrahedral symmetry.

59 ntermediate product of dioxygen reduction in L-chain as well as in H-chain and H-chain variant ferrit

60 ormation, accumulation of F-actin and myosin L chain at the leading edge, and accumulation of phospho

61 Likewise, in the BALB/c.56R mouse, dual L chain B cells are found in the follicular B cell compa

62 The Jkappa usage in dual kappa L chain B cells suggests increased receptor editing acti

63 g pattern, which does not associate with the L chain (beta(2)-microglobulin).

64 competition and species origin of the MHC-I L chain (beta2-microglobulin).

65 Co-transfection of cells with both H- and L-chain cDNAs increased the intracellular levels of H-ch

66 The "escaped" dual-L-chain cells and the "ignored" low-affinity cells are t

67 These H- and L-chain characteristics were not identified in other B-C

68 This study of a large family of kappa L chain clusters in nurse shark completes the characteri

69 Only one H/L chain combination bound neutral PL and none bound dsDN

70 Eleven of 14 H/L chain combinations displayed weak binding to OVA with

71 Binding of the expressed H/L chain combinations to a range of anionic, neutral, and

72 "double-producers," suggesting that H chain/L chain combinations with superior signaling properties

73 gions of the heavy (V(H)) and kappa-light (V(L)) chain complementary DNA (cDNA) of anti-GD2 IgM hybri

74 recursor B cell receptors with the surrogate L chain complex that promotes allelic exclusion but not

75 f Ig mu heavy chains (HCs) and the surrogate L chain components lambda5 and VpreB are critical for B

76 These L-chain-containing inclusion bodies were found in the cy

77 ls, we show that secretion-competent lambda1 L chains could associate with both full-length H chains

78 d Dmu, whereas secretion-incompetent lambda1 L chains could only do so with full-length H chains.

79 ng and analyzing two comprehensive murine Ig L chain databases, one consisting of 264 monoclonal ANAs

80 rtant, although not essential, for surrogate L chain-dependent receptor signaling in primary cells, a

81 The ferritin L-chain detected in both lens types was modified and was

82 These L chains diminish or veto anti-DNA binding when expresse

83 isted in the ancestral Ig gene, before H and L chain divergence.

84 ndrome, which is caused by overexpression of L-chain due to mutation in the regulatory element in the

85 L chain-edited transgenic B cells were detectable in spl

86 Although TLR9 is dispensable for L chain editing during B cell development in the bone ma

87 sbb2(a) does not alter L chain editing frequencies of DNA Abs in the 3H9H/56R H

88 We conclude that L chain editing in anti-DNA-transgenic B cells is not on

89 Finally, we also report evidence for ongoing L chain editing in sIgM(low) transitional splenic B cell

90 otypic analysis reveals B cell depletion and L chain editing in Tg mice.

91 ding an autoreactive Ab, we reported ongoing L chain editing not only in bone marrow cells with a pre

92 gers recombination-activating gene activity, L chain editing, and deletion.

93 eted) H chain locus and evidence of vigorous L chain editing; Abs isolated from B6.Sle2(z).56R spleen

94 to SCID mice resulted in the appearance of a L chain editor (Vlambdax) in the serum of engrafted reci

95 In this study, we show that the NFkappa L chain enhancer of activated B cells 2 (NFkappaB2)/p100

96 point to possible involvement of cytoplasmic L-chain-enriched ferritin aggregates in the formation of

97 r of the L-chain resulted in accumulation of L-chain-enriched ferritin associated with cytoplasmic in

98 ed canonical, expressing a particular Vkappa L chain, evade central tolerance by down-regulating BCR

99 We ask whether a similar set of L chains exists in the human repertoire, and if so, do t

100 al role in promoting the formation of lambda L chain-expressing B cells.

101 IL and BLyS partially colocalized with kappa L chain-expressing B-lineage cells at the epithelial-con

102 We hypothesize that reduced surrogate L chain expression contributes to decreased pre-B cells

103 requency, suggesting VprBP does not regulate L chain expression from a productively rearranged Igk al

104 he timing of BBB permeability changes, kappa-L chain expression in CNS tissues, and Ab production in

105 levated CD19 levels were paralleled by kappa-L chain expression levels.

106 :mCherry(+) cells and the timing of Ig H and L chain expression revealed simultaneous expression of b

107 , higher levels of CD43, and decreased kappa L chain expression.

108 erate new B cells with altered phenotype and L chain expression.

109 4-hydroxy-3-nitrophenylacetyl) but differ in L chain expression.

110 correlates with Ig lambda (Iglambda) light (L) chain expression and likely plays a role in receptor

111 We show that L chain ferritin is undetectable in primary fibroblasts

112 dase site mutant 222 (H62K + H65G) and human L-chain ferritin (HuLF) lack this activity.

113 o types, ferritin H chain (FHC) and ferritin L chain (FLC).

114 e, and accumulation of phosphorylated myosin L chain, flotillin-2, and P-selectin glycoprotein ligand

115 HspB5 also precipitated Ig heavy and L chains from sera from patients with MS.

116 ligand-binding domain, the I domain of alpha(L) chain, from an inactive, low-affinity closed form (LA

117 nd in the B cell subpopulations, an order of L chain gene activation is suggested as: sigma-2 followe

118 , autoreactive B cells may undergo secondary L chain gene rearrangement (receptor editing) and change

119 nation of IL-7 signals and the initiation of L chain gene rearrangement remains to be elucidated.

120 which leads to G1 arrest and induction of Ig L chain gene rearrangement, respectively.

121 (change) their specificity by secondary H or L chain gene rearrangement.

122 lacement for a functional V kappa 8J kappa 5 L chain gene.

123 and in two classes of mutations in the human L-chain gene, resulting in hereditary hyperferritinemia

124 , used a broad L chain repertoire, including L chains generating high-affinity autoreactivity, and pr

125 To understand the derivation of these L chain genes and their organizations, we performed phyl

126 uring B lymphocyte development, Ig heavy and L chain genes are assembled by V(D)J recombination.

127 tation events are very similar at both H and L chain genes in this early vertebrate.

128 nd to be highly diverse, with the profile of L chain genes isolated from whole pancreas differing fro

129 Stage-specific rearrangement of Ig H and L chain genes poses an enigma because both processes use

130 Analysis of BCR L chain genes was used to investigate selection of B lym

131 Graves' disease, which shared the same H and L chain germline gene rearrangements and then diversifie

132 tion between the V gene segments of Ig H and L chains has been noted previously by several investigat

133 Overexpression of the L-chain has been associated with the formation of premat

134 ntain varying ratios of heavy (H) and light (L) chains; however, known ferritin structures include on

135 ceptor in dual receptor B cells is an editor L chain, i.e., neutralizes or alters self-reactivity of

136 copies of the unrearranged human H chain and L chain Ig gene loci.

137 , designated SMI, expressing unmutated H and L chain Ig genes encoding a low-affinity, polyreactive h

138 irtually all B lymphocytes express the H and L chain Ig molecules from the intra-islet-derived anti-p

139 an repress the expression of a transgenic Ig L chain (IgL) by doxycycline (IgL-repressible mouse).

140 GMDV (motif-1), almost invariably express Ig L chains (IgL) encoded by IGLV3-21, whereas CLL that use

141 cteristic for MZ B cells was altered and the L chain Iglambda(+) repertoire was reduced in bumble mic

142 and IL-17A-induced phosphorylation of myosin L chain in HPKs, which is a major regulator of the actom

143 5 H clones were expressed with wild-type T15 L chain in vitro.

144 ges in the relative orientation of the H and L chains in both the variable and constant domains.

145 the VHH side, which normally associates with L chains in conventional antibodies.

146 ely results, in part, from reduced surrogate L chains in senescent B cell precursors and compromised

147 heightened expression of endogenous Ig H and L chains in splenic B cells, upregulation of RAG, and se

148 This model supports a role for the L-chain in iron mineralization and helps to explain the

149 caused significant accumulation of ferritin L-chain in the cells.

150 Ferritin H- and L-chains in canine and human fiber cells of healthy lens

151 ransfected with cDNAs for dog ferritin H- or L-chains in order to study differential expression of th

152 expressed the Ig variable regions of several L chain-included B cells in cell culture.

153 We find that although the number of L chain-included B cells increases as a result of recept

154 V(H)3H9 yields an anti-DNA Ab with most L chains including an anti-ssDNA with the V(kappa)8 Tg a

155 ing autoreactivity are especially subject to L chain inclusion.

156 ar adhesion molecule 1 (ICAM-1) by its alpha(L)-chain inserted domain (I-domain).

157 sulted in human VH pairing with mouse lambda L chains instead of allowing otherwise subdominant V2-gl

158 The H/L-chain interface includes complementary features respon

159 How surrogate and conventional L chains interpret Dmu's unusual structure and how that

160 uld not form receptors with a panel of kappa L chains irrespective of their secretion properties.

161 glycolipid-mCD1d complex, although only the L chain is involved in contacts with the glycolipid anti

162 ppa genes are consumed, and 3) the surrogate L chain is necessary for selection of productive IgH gen

163 k of competing cis interactions, 2) tetramer L chain is of mouse origin, and 3) Ly49 is expressed in

164 (H) chain immunoglobulin (Ig) without light (L) chain is prevented by chaperone association of the H

165 licated in the interaction between the H and L chains, is often intrinsically disordered, and is invo

166 In conclusion, a shark B cell expresses one L chain isotype at the surface and other isotypes as non

167 esults provide insight into the mechanism of L chain isotype exclusion and indicate that RS has a phy

168 genomic organization of the three zebrafish L chain isotypes and found they all differed from those

169 Two of the zebrafish L chain isotypes are encoded by two loci, each carrying

170 nes encoding one of the three nurse shark Ig L chain isotypes, called NS5.

171 two H chain isotypes, mu and omega, and four L chain isotypes, kappa, lambda, sigma, and sigma-2).

172 Nurse sharks express four L chain isotypes, kappa, lambda, sigma, and sigma-2, enc

173 for mutants from six loci of two nurse shark L chain isotypes, showing that somatic hypermutation eve

174 nantly nonproductive rearrangements of other L chain isotypes.

175 kappa clusters compared with the other three L chain isotypes.

176 Most mature B lymphocytes express one BCR L chain, kappa or lambda, but recent work has shown that

177 attached to its binding motif on the myosin L chain kinase (MLCK) promoter region, leading to the ac

178 Specifically, the role of myosin L chain kinase (MLCK) was investigated.

179 activation induced phosphorylation of myosin L chain kinase and regulatory myosin L chain.

180 The L chain lacks the residues that form a putative iron cor

181 56R/kdel mice only produce four L chains (lambda1, lambda2, lambda3, and lambdaX).

182 ymphocyte function-associated antigen 1alpha(L) chain (LFA-1alpha(L)) in these patients.

183 essible at the pro-B cell stage, whereas the L chain loci become accessible at the pre-B cell stage.

184 ce expressing, in the physiological Ig H and L chain loci, two well-studied broadly neutralizing Abs:

185 receptor editing rearrangements at Ab H and L chain loci.

186 F4,8 may directly regulate the activation of L chain loci.

187 n SHM reporter cassette inserted into the Ig L chain locus (IgL) of chicken DT40 B cells, we have ide

188 The Ig kappa L chain locus contains multiple enhancers, including the

189 L L chain was "knocked-in" at the endogenous L chain locus.

190 to more strictly control cytosolic levels of L-chain may augment its accumulation in lenses of humans

191 s (ECs), activation of ROCK regulates myosin L chain (MLC) phosphorylation, stress fiber formation an

192 inheritance of the transgene-encoded Vkappa8 L chain, most likely a neoantigen created by the inserti

193 B6.56R mouse, polyreactive B cells with dual L chain move to the follicular B cell compartment.

194 ing indicated that non-stochastically paired L chain of IGLV3-9 contributes to the antigen binding of

195 Interestingly, the L chains of ANAs exhibited differential usage of certain

196 or the kappa (Igkappa) and lambda (Iglambda) L chains of ferret Ig.

197 r signatures, it remains unclear whether the L chains of these Abs also possess distinctive molecular

198 The expressed heavy (H) or light (L) chain of an autoreactive receptor is replaced by upst

199 HCAbs fail to incorporate light (L) chains owing to the deletion of the first constant do

200 as most current protocols do not preserve H:L chain pairing.

201 domization of cognate heavy (H) chain/light (L) chain pairing, which could occur to a varying extent

202 transgenic H chain paired with a spectrum of L chains, predominantly recombined to J(k)1 or J(k)2.

203 A Vkappa14 L chain predominated within the complex pancreatic reper

204 with decreased expression and processing of L chain protein 3 (LC3), a key component of the autophag

205 L) chain recombination and cytoplasmic kappa L chain protein expression.

206 the chains was used to alter the ferritin H:L chain ratio and to determine the effect of these chang

207 lts in the reinduction of genes required for L chain rearrangement and receptor editing.

208 nt activity at the kappa locus, and promotes L chain rearrangement and transcription.

209 y be a result not only of simultaneous H and L chain rearrangement in the shark but also of processin

210 dsDNA breaks indicative of ongoing secondary L chain rearrangement not only in bone marrow cells with

211 up-regulation of MHC class II, and augmented L chain rearrangement, resulting in a successful transit

212 ration index that may indirectly inhibit the L chain rearrangement.

213 hat alters BCR specificity through continued L chain rearrangement.

214 suggest that central tolerance and attendant L chain receptor editing affect a large fraction of norm

215 sulted in retarded clonotype development and L chain receptor editing in vivo.

216 se mice demonstrated extensive and efficient L chain receptor editing responses and had B cell number

217 ransgene, when paired with endogenous lambda L chain, recognizes the hapten 4-hydroxy-3-nitro-phenyl

218 -BCR, Igkappa germline transcription, and Ig L chain recombination.

219 J(H) recombination, and induced kappa light (L) chain recombination and cytoplasmic kappa L chain pro

220 This shift in L chain repertoire was accompanied by inhibition of deve

221 9 mice were elevated in number, used a broad L chain repertoire, including L chains generating high-a

222 only subtle effects on the glD42H-associated L chain repertoire.

223 ilar restriction characterized the preimmune L chain repertoire.

224 In contrast, slower turnover of the L-chain resulted in accumulation of L-chain-enriched fer

225 L-chain-rich ferritin in L-chain-transfected cells forme

226 Expression of a short hairpin RNA for kappa L chain RNA in B cells resulted in reduction in levels o

227 Multireactivity, allelic inclusion, and L chain secretion are three consequences of editing at t

228 Forced overexpression of free L chain secretion reinstated tumor rejection.

229 Up to 75% of the amplified H- and L-chain sequences were contained in overrepresented popu

230 with an approximately 50% framework-reverted L chain showed a 2-fold improvement in potency over the

231 iron in its Fe3+ oxidation state, while the L chain shows iron nucleation properties.

232 o all human L chains and found several human L chains similar to mouse editors.

233 Both H and L chain site-directed transgenic mice show increased B c

234 h and in frequency than any other vertebrate L chain so far reported and rivals that in H chain.

235 n the basic peak consisted of two unmodified L chain subunits and a single H chain ending in glycine,

236 FLsiRNA decreased both ferritin H- and L-chain synthesis.

237 itin H-chain synthesis, but doubled ferritin L-chain synthesis.

238 Certain L chains, termed editors, rescue anti-DNA B cells by neu

239 of mineralization more characteristic of an L-chain than an H-chain ferritin.

240 ertebrate (gnathostome) and is the essential L chain that associates with most MHC class I molecules.

241 In contrast, glD42H B cells expressing L chains that confer high-affinity autoreactivity are mo

242 eased glD42H NZB/W mice is dominated by five L chains that confer no or low-affinity polyreactivity.

243 ssed the 56R H chain transgene with "editor" L chains that did not completely veto autoreactivity.

244 cells from 3H9;RS(-/-) mice were enriched in L chains that promote DNA binding.

245 Light (L) chains that edit anti-DNA heavy (H) chains rescue B-c

246 nt on the lambda5 component of the surrogate L chain, the development of B cells expressing the tg al

247 his H chain, when combined with its original L chain, the lambda1 L chain, yields a NAA that characte

248 te was present in CDR1, CDR2, or CDR3 of the L chain, the V(H) CDR2 glycan remained high mannose.

249 anti-laminin Ig in combination with multiple L chains, the H + L Ig binds ssDNA in addition to lamini

250 When combined with surrogate or conventional L chains, these V(H)14 IgH chains did not provide increa

251 -transgenic mice homozygous for the targeted L chain to exclude secondary kappa rearrangements result

252 Libraries of Vkappa L chain transcripts were generated from the spleens of c

253 L-chain-rich ferritin in L-chain-transfected cells formed inclusion bodies that w

254 round, expression of a site-directed Igkappa L chain transgene increases Igkappa(+) B cell frequency,

255 This is the second nurse shark L chain type where both germline-joined and split V-J or

256 B cells impairs selection of Igkappa editor L chains typically arising through secondary Igk rearran

257 e manner in which B cells with lambda light (L) chains undergo receptor editing, we have studied hybr

258 essing B cells and was minimally affected by L chain usage for most V(H).

259 ch, and an inverted lambda/kappa ratio of Ig L chain usage indicated that a local differentiation pro

260 gkappa and Iglambda in serum and to evaluate L chain usage of the Ab response against the hemagglutin

261 toimmunity in a polyclonal immune system, Ig L chain usage was analyzed in 3H9 site-directed IgH chai

262 HA exhibited an inherent bias toward lambda L chain usage.

263 ithin distinct PC subsets exhibited distinct L chain usage.

264 B cells and sequenced the unexpressed kappa L chains using next-generation methods.

265 ypical IgG(+) MBCs, we compared the Ab H and L chain V gene repertoires of children living in a malar

266 Second, H and L chain V gene use seems to be biased, particularly amon

267 idual 4 years before FS onset, and the H and L chain V genes of anti-DSG1 autoantibodies were analyze

268 rogation of paired H chain V region (VH) and L chain V region (VL) sequences of individual and Ag-spe

269 (BCRs) arising from the use of common H and L chain V region gene segments that share CDR3 structura

270 We sequenced Ab H and L chain V region genes (V(H) and V(L)) of clones expande

271 ific, HIV neutralizing murine IgM with H and L chain V regions (V(H) and V(L) regions) free of immuno

272 traint in the primary structure of the H and L chain V regions exist, the possibility that this level

273 We sequenced both H and L chain V(D)J rearrangements from 366 CLL patients and m

274 Germline H- and L-chain V gene Abs generated according to mutated cross-

275 ikingly similar BCRs (use of the same H- and L-chain V gene segments with unique, shared heavy chain

276 reactivity was through the use of the editor L chains, V(k)20 or V(k)21.

277 ere encoded by distinct H chain variable and L chain variable gene segment rearrangements and possess

278 carrying functionally rearranged H chain and L chain variable region genes isolated from a B cell hyb

279 mprises associated heavy (V(H)) and light (V(L)) chain variable domains, but in camels and llamas, th

280 e and genetic origin of the endogenous kappa L chain (Vkappa or IgL) repertoire that pairs with the V

281 ecrete a monoclonal Ig containing a V region L chain (VL) epitope recognized by CD4+ T cells.

282 brane HEL in a setting in which the anti-HEL L chain was "knocked-in" at the endogenous L chain locus

283 ed sequence, while secretion of surplus free L chain was severely diminished.

284 g either human or murine beta2-microglobulin L chains was tested for all five Ly49 receptors in all f

285 Canine L-chain was approximately 11 kDa larger than standard ca

286 This excess ferritin L-chain was found in inclusion bodies, some of which wer

287 at 48 h after transfection overexpression of L-chain was much higher (9-fold over control) than that

288 than standard canine L-chain, whereas human L-chain was of the proper size.

289 Although free L chains were efficiently processed and presented by tum

290 Two predominant L chains were identified in B cells that bind heterologo

291 Conversely, L chains were insufficient to refine H chain-based clona

292 containing coding sequences for either H- or L-chains were used to modify ferritin subunit makeup.

293 oximately 11 kDa larger than standard canine L-chain, whereas human L-chain was of the proper size.

294 FHsiRNA blocked the degradation of ferritin L-chain, which caused significant accumulation of ferrit

295 Coexpression of secretion-restored mutant L chains with the secretion-defective mutant H chains re

296 S 120-kDa IgY comprising two equally sized H/L chains with truncated Fc and a 7S 200-kDa IgY comprise

297 e bond releasing and activating the captured L-chain with subsequent specific cleavage of the SNAP25(

298 in vertebrates comprise equally sized H and L chains, with exceptions such as H chain-only Abs in ca

299 d around CDRs and the DE loop for both H and L chains, with similar frequencies for healthy donors an

300 bined with its original L chain, the lambda1 L chain, yields a NAA that characteristically binds a va