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1 L. pneumophila adhesion on these biofilm under low flow
2 L. pneumophila delivers almost 300 effector proteins int
3 L. pneumophila encodes more than 300 putative effectors,
4 L. pneumophila is able to infect alveolar macrophages in
5 L. pneumophila is ubiquitously found in freshwater envir
6 L. pneumophila is well known as the cause of Legionnaire
7 L. pneumophila lacking lppA replicated less efficiently
8 L. pneumophila mutant strains lacking EnhC (DeltaenhC) i
9 L. pneumophila pathogenicity relies on secretion of more
10 L. pneumophila replicates in protozoa and mammalian phag
11 L. pneumophila replicates within macrophages by using a
12 L. pneumophila serogroup 1 isolates (n = 106) from the s
13 L. pneumophila strain Lp02, which is attenuated in the a
14 L. pneumophila TLR ligands induced the splicing of mRNA
15 L. pneumophila translocates more than 300 effectors into
16 L. pneumophila triggers the reduction of several sphingo
17 L. pneumophila was able to inhibit both chemical and bac
18 L. pneumophila were not detected by either method; Legio
21 , we report the sequence and analyses of 364 L. pneumophila genomes, including 337 from the five dise
22 er treatment to characterize and eliminate a L. pneumophila population responsible for nosocomial inf
23 t Ab-mediated protection is effective across L. pneumophila serogroups, suggesting that Abs specific
29 y in response to iron limitation that allows L. pneumophila to abandon the host cell when nutrients a
33 ocated inefficiently into cultured cells, an L. pneumophila DeltalpdA mutant displayed reduced replic
34 n among disinfectant residual, biofilms, and L. pneumophila, which provides guidelines to assess and
36 racellular colocalization of B. neotomae and L. pneumophila was required for rescue and that colocali
37 ring the wet season than the dry season, and L. pneumophila was only observed during the wet season.
38 TBK1/IRF3 pathway during M. tuberculosis and L. pneumophila infection of macrophages, whereas L. mono
39 to recognize intracellular bacteria such as L. pneumophila, leading to potent inflammatory responses
40 sinfectant to release the biofilm-associated L. pneumophila from these two types of biofilms, the L.
41 his study, the release of biofilm-associated L. pneumophila under simulated drinking water flow conta
44 hat LtpD is a novel phosphoinositide-binding L. pneumophila effector that has a role in intracellular
46 ously derived rhizoferrin are assimilated by L. pneumophila in an LbtU- and LbtC-dependent manner.
47 ytokines in experimental pneumonia caused by L. pneumophila Intratracheal (i.t.) administration of L.
50 creens reveal known host factors hijacked by L. pneumophila, as well as genes spanning diverse traffi
51 solized bacteria, and infection of humans by L. pneumophila can result in a severe pneumonia called L
54 However, the infection event mediated by L. pneumophila Cas2 appeared to be distinct from this fu
56 We show here an iron starvation response by L. pneumophila after infection of macrophages that was p
57 We now report that two molecules secreted by L. pneumophila, homogentisic acid (HGA) and its polymeri
58 ious proteomic analysis revealed that T2S by L. pneumophila 130b mediates the export of >25 proteins,
59 d molecular mimicry strategy that is used by L. pneumophila to take control of the host cell Hippo pa
60 troscopy to directly sort pellets containing L. pneumophila cells, expelled by T. thermophila, and to
64 al structure and local hydrodynamics control L. pneumophila adhesion to and detachment from simulated
66 spase-11-dependent pyroptosis by cytoplasmic L. pneumophila-derived LPS required Gbp(chr3) proteins.
67 ng bacterial products into the host cytosol, L. pneumophila also activates cytosolic immunosurveillan
70 PlcA and PlcB are two previously defined L. pneumophila proteins with homology to the phosphatidy
71 fections with the cytosol-invading DeltasdhA L. pneumophila mutant was similarly dependent on Gbp(chr
73 y, two primer/probe sets (one able to detect L. pneumophila and the other L. pneumophila Sg1) were de
77 the gene encoding LegC4 resulted in enhanced L. pneumophila in the lungs of infected mice but not wit
78 enhanced the capacity of viable filamentous L. pneumophila to escape phagosomal killing in a length-
82 The metaeffector Lpg2505 is essential for L. pneumophila intracellular replication only when its c
85 ion in vitro Moreover, LbtP is important for L. pneumophila growth within macrophages while LbtU is d
88 n Rab6A' function and the role of Rab6A' for L. pneumophila growth within host cells has been unclear
89 colony-forming units (CFU) per reaction for L. pneumophila and three CFU per reaction for S. typhimu
90 Thus, the T2SS is absolutely required for L. pneumophila to grow to larger numbers in its intravac
91 hils are both an intracellular reservoir for L. pneumophila and a source of proinflammatory cytokines
92 9, was previously identified in a screen for L. pneumophila IDTS that manipulate secretory traffic wh
93 ar isotopologue patterns in amino acids from L. pneumophila wild type and the mutants under study ref
94 of the immunoglobulin-cleaving protease from L. pneumophila revealed that the protease is conserved a
95 ify the LegC3 secreted effector protein from L. pneumophila as able to inhibit a SNARE- and Rab GTPas
96 oiled-coiled domain containing proteins from L. pneumophila, LegC7/YlfA and LegC2/YlfB, did not inhib
98 we show that the effector protein VipD from L. pneumophila exhibits phospholipase A1 activity that i
99 environmental survival and virulence; e.g., L. pneumophila employs T2S for infection of amoebae, gro
103 erestingly, we show that the host identifies L. pneumophila infection as a form of endoplasmic reticu
104 , loss of both LbtP and LbtU does not impair L. pneumophila growth in the amoebal host Acanthamoeba c
105 Although the ability of IFN-gamma to impede L. pneumophila growth is fully dependent on Stat1, IFN-a
109 approach to interfere with DsbA2 function in L. pneumophila determined that DSB oxidase activity was
116 -based typing (SBT) analysis of all incoming L. pneumophila serogroup 1 (Lp1) isolates to identify po
117 delay in lung bacterial clearance, increased L. pneumophila dissemination to extrapulmonary organs, a
119 ba amino acids into the LCV and further into L. pneumophila where they served as precursors for bacte
120 alth of previously undescribed insights into L. pneumophila pathogenesis and mammalian cell function.
124 pellets, detection methods for packaged live L. pneumophila forms remaining in water should be cultiv
126 Furthermore, approximately <10 CFU per mL L. pneumophila may be appropriate for healthcare or susc
129 infection with wild-type but not T4SS mutant L. pneumophila Using confocal microscopy, it was determi
131 cterial supernatants enhanced the ability of L. pneumophila and other species of Legionella to take u
132 '(Q72L) significantly reduced the ability of L. pneumophila to initiate intracellular replication in
133 enic evolution and nutritional adaptation of L. pneumophila and other intracellular bacteria to life
134 and macrophages, we show that adaptation of L. pneumophila to each amoeba causes the accumulation of
135 phila Intratracheal (i.t.) administration of L. pneumophila induced the upregulation of both IL-36alp
138 together, genome-wide chromatin analysis of L. pneumophila-infected macrophages demonstrated inducti
141 tematically comparing pulmonary clearance of L. pneumophila in C57BL/6 MyD88(-/-), TLR2(-/-), TLR3(-/
142 document the occurrence and colonization of L. pneumophila Sg1 in cold water delivered from point of
144 olling adhesion and subsequent detachment of L. pneumophila associated with biofilms remain unclear.
146 viable Legionella, shown with the example of L. pneumophila, ranging in a total concentration between
147 ost cell infection, VipD reduces exposure of L. pneumophila to the endosomal compartment and protects
148 e role of two important virulence factors of L. pneumophila, the potent danger signal flagellin and t
150 that members of the SidE effector family of L. pneumophila ubiquitinate multiple Rab small GTPases a
153 with iron to enhance intracellular growth of L. pneumophila DeltamavN strains, indicating a clear rol
155 etectable effects on intracellular growth of L. pneumophila within macrophages or amebae, the lack of
159 yses indicate that environmental isolates of L. pneumophila have a potential positive selection for t
161 ) hospital plumbing contained high levels of L. pneumophila; c) Legionella control measures in hospit
165 ectious mature intracellular forms (MIFs) of L. pneumophila are considered as infectious particles mo
166 can be easily adapted for the monitoring of L. pneumophila serogroups in clinical and environmental
168 Genetic screening using flagellin mutants of L. pneumophila as a surrogate host, reveals a novel C. b
169 Here we show the aroB and aroE mutants of L. pneumophila to be defective in growth in human monocy
172 This review aims to provide an overview of L. pneumophila pathogenesis in the context of the host i
173 th a role in the ecology and pathogenesis of L. pneumophila, HGA and HGA-melanin were effective at re
174 s not been characterized in the pathology of L. pneumophila TNFAIP2 messenger RNA and protein were up
176 arly half of the taps showed the presence of L. pneumophila Sg1 in one sampling event, and 16% of tap
180 ntly, we determined that the Cas2 protein of L. pneumophila promotes intracellular infection of Acant
181 The bona fide F-box AnkB effector protein of L. pneumophila strain AA100/130b is anchored to the cyto
185 ot required for intracellular replication of L. pneumophila, RavD is a part of the molecular mechanis
189 into a phase associated with restriction of L. pneumophila Furthermore, control of cyclin D1 may be
194 he vacuole was observed by using a strain of L. pneumophila in which all of the effector proteins in
196 its catalytic mutant form, into a strain of L. pneumophila that naturally lacks a CRISPR-Cas locus c
197 re conducted with a RavZ-deficient strain of L. pneumophila, L. monocytogenes was targeted by the hos
198 pneumophila subsp. pneumophila, 3 strains of L. pneumophila subsp. fraseri or L. pneumophila subsp. p
199 ionella species or subspecies: 15 strains of L. pneumophila subsp. pneumophila, 3 strains of L. pneum
200 Here, we determined the crystal structure of L. pneumophila RidL in complex with the human VPS29-VPS3
201 or phtD(+) alleles enhanced the survival of L. pneumophila thymidylate synthase (thyA)-deficient str
204 genes of E. coli were replaced with those of L. pneumophila, motility was restored and DsbA2 was pres
205 This observation highlights the utility of L. pneumophila as a powerful tool for studying a critica
207 its chaperone RABIF are required for optimal L. pneumophila replication and ER recruitment to the LCV
208 strains of L. pneumophila subsp. fraseri or L. pneumophila subsp. pascullei, 4 strains of "L. donald
209 cisella novicida, but unlike R. pickettii or L. pneumophila, Francisella species lack putrescine bios
210 domains are encoded in the genomes of other L. pneumophila isolates and species, suggesting that Ltp
211 able to detect L. pneumophila and the other L. pneumophila Sg1) were determined to be highly sensiti
212 spp. densities (likely including pathogenic L. pneumophila) were significantly higher in one type of
215 risks of pathogenic Legionella pneumophila (L. pneumophila), thus raising human health concerns.
217 iminated culturable Legionella and prevented L. pneumophila from recolonizing biofilms, but M. avium
218 ose that PhtC and PhtD contribute to protect L. pneumophila from dTMP starvation during its intracell
223 ound a superior role of IgG2c in restricting L. pneumophila replication in a prophylactic setting.
226 enetic element profiles, suggesting a single L. pneumophila population as the source of nosocomial in
227 mimicked thymidine limitation or starvation, L. pneumophila exhibited a marked requirement for PhtC f
230 Stat1, IFN-alphabeta unexpectedly suppresses L. pneumophila growth in both Stat1- and Stat2-deficient
232 tests with E. coli mutants established that L. pneumophila dsbA1, but not the dsbA2 strain, restored
237 lacking phtC or phtD strongly indicate that L. pneumophila requires PhtC and PhtD function under con
241 Furthermore, biochemical studies reveal that L. pneumophila uses two effectors (Lgt1 and Lgt2) to inh
250 ophila from these two types of biofilms, the L. pneumophila release kinetics values from predisinfect
256 gether, our study provides evidence that the L. pneumophila effector GobX exploits two post-translati
261 is suggests a complex mechanism by which the L. pneumophila effector SidJ modulates the function of t
262 de novo assembly allowed comparison with the L. pneumophila Paris reference strain to infer phylogene
264 nding interface on Rab5, explaining why this L. pneumophila effector can compete with cellular ligand
267 large cohort of effectors that contribute to L. pneumophila virulence positively or negatively and ha
270 estimated risks of illness from exposure to L. pneumophila and MAC via showering were generally low
273 al initiator of the inflammatory response to L. pneumophila in vivo and point to an important role fo
274 and protein were upregulated in response to L. pneumophila infection of human-BDMs and human alveola
275 tral role in the transcriptional response to L. pneumophila METHODS: We infected human-blood-derived
278 contrast, the Sec-7 domain was restricted to L. pneumophila and seven other species, indicating effec
280 host immunity and reveal a T4SS-translocated L. pneumophila phytase that counteracts intracellular ba
282 Interference with AnkB function triggers L. pneumophila to exhibit a starvation response and diff
283 e able to promote Fe(3+) uptake by wild-type L. pneumophila as well as enhance growth of iron-starved
287 utrophil recruitment in response to virulent L. pneumophila requires the production of IL-1alpha spec
289 ria to be inhaled into the human lung, where L. pneumophila can be phagocytosed by alveolar macrophag
290 us, there are at least two pathways by which L. pneumophila can disrupt xenophagic targeting of the v
292 ranes for biogenesis of the vacuole in which L. pneumophila replicates, these studies have revealed t
294 flexneri intercepts all host pyruvate, while L. pneumophila induces host protein degradation and bloc
295 human-blood-derived macrophages (BDMs) with L. pneumophila and used chromatin immunoprecipitation fo
296 se 13C-prelabeled amoebae were infected with L. pneumophila wild type or some mutants defective in pu
300 evealed that hospitals have been seeded with L. pneumophila via both local and international spread o