ライフサイエンスコーパス: Legionella

1 nd is caused by infection with the bacterium Legionella.

2 e between potential and confirmed sources of Legionella.

3 owledge about all the (potential) sources of Legionella.

4 could be classified as confirmed sources of Legionella.

5 ymes on the surface of vacuoles that contain Legionella.

6 by the French National Reference Center for Legionella (2013-2017) including cases of slowly or nonr

7 Legionella, a large group of environmental Gram-negative

8 tified stagnant pipes, inhibiting culturable Legionella and biofilm formation, but promoted Legionell

9 By using several species of Legionella and mice singly deficient for caspase-1 or ca

10 amines, on water chemistry and the growth of Legionella and mycobacteria across a transect of bench-

11 confirms prior reports of trade-offs between Legionella and mycobacteria if chloramines are applied a

12 aterborne pathogens were bacteria, including Legionella and other gram-negative bacteria, and nontube

13 Chloramines eliminated culturable Legionella and prevented L. pneumophila from recolonizin

14 bers of P&I hospitalizations attributable to Legionella (and influenza and RSV) by age group, season,

15 egionella), decorative water wall fountains (Legionella), and heater-cooler devices used in cardiac s

16 Water is the major natural reservoir for Legionella, and the pathogen is found in many different

17 available evidence on the seroprevalence of Legionella antibodies and explores factors that may infl

18 breaks within the last years have shown that Legionella are a growing challenge for public health.

19 Water quality guidance values for Legionella are available for building managers but are g

20 bers of P&I hospitalizations attributable to Legionella are comparable to those provided by etiologic

21 ylococcus aureus, Chlamydia, Mycoplasma, and Legionella are each identified in 2%-5% of patients with

22 ethods capable of rapidly identifying viable Legionella are important for the control of engineered w

23 ntified in early 1977, bacteria of the genus Legionella are recognised as a common cause of community

24 Legionella bacteria are ubiquitous in natural matrices a

25 e pneumonia and systemic infection caused by Legionella bacteria is Legionnaires' disease.

26 Legionella bacteria multisystem manifestations mainly af

27 rtant role in the transmission of infectious Legionella bacteria; they might not yet be considered in

28 between 6 and 10 mg/L, reducing the level of Legionella by three logarithmic unit by 2 months postins

29 Legionella can cause Legionnaires' disease, a potentiall

30 We propose that established Legionella communities may deploy molecules such as HGA

31 The genus Legionella comprises 65 species, among which Legionella

32 Cell cycle arrest upon Legionella contact is dependent on the Icm/Dot secretion

33 ch is localized to the cytosolic side of the Legionella-containing vacuole (LCV) and is essential for

34 y hijacking endocytic pathways and forming a Legionella-containing vacuole (LCV) in which the bacteri

35 Within macrophages and amoeba, the Legionella-containing vacuole (LCV) membrane is derived

36 establish an endoplasmic reticulum (ER)-like Legionella-containing vacuole (LCV) that supports bacter

37 ostentry and to grow to large numbers in the Legionella-containing vacuole (LCV), as evident at 12 h.

38 stablish a replication-permissive niche, the Legionella-containing vacuole (LCV).

39 nia by replicating within macrophages in the Legionella-containing vacuole (LCV).

40 ise unrelated effectors, targets LtpM to the Legionella-containing vacuole and to early and late endo

41 into macrophages, RavD was retained onto the Legionella-containing vacuole and was also present on sm

42 t of the molecular mechanism that steers the Legionella-containing vacuole away from endolysosomal ma

43 ocalization N) protein to the surface of the Legionella-containing vacuole where this putative transm

44 Rtn4 playing a role in the formation of the Legionella-containing vacuole, it was recruited to almos

45 d establish an endoplasmic reticulum-derived Legionella-containing vacuole, which facilitates bacteri

46 R) fragmentation and membrane recruitment to Legionella-containing vacuoles (LCV) emerged as major Si

47 We report that the delivery of GBP2 to Legionella-containing vacuoles is dependent on the bacte

48 Vs and that the delivery of GBP1 and GBP2 to Legionella-containing vacuoles or YCVs is substantially

49 e factors, become flagellated, and leave the Legionella-containing vacuoles.

50 hase, the bacteria grow within host cells in Legionella-containing vacuoles.

51 iation of PR-ubiquitinated host targets with Legionella-containing vacuoles.

52 is a promising disinfectant that can prevent Legionella contamination of hospital water supplies.

53 contained high levels of L. pneumophila; c) Legionella control measures in hospital plumbing aligned

54 ve the manufacturer's recommended target for Legionella control.

55 of the core and accessory components of the Legionella core transmembrane subcomplex, revealing a we

56 ospital, including collection of samples for Legionella culture.

57 or nonresolving LD was diagnosed on positive Legionella cultures (n = 10, 83.3%) at 49.5 (IQR, 33.7-7

58 ectronic faucets (Pseudomonas aeruginosa and Legionella), decorative water wall fountains (Legionella

59 this issue of Cell Host & Microbe, show that Legionella deploys a novel form of ubiquitylation to gen

60 uted tomography (CT) scan abnormalities, and Legionella detection in lower respiratory tract specimen

61 4SS-dependent interplay between Brucella and Legionella during macrophage coinfection.

62 our Legionella effector screen, we used the Legionella effector LepB lipid kinase to confirm the cri

63 Recently, the Legionella effector MavC was found to mediate a unique u

64 vgA, and that this pentameric assembly binds Legionella effector proteins.

65 To further validate our Legionella effector screen, we used the Legionella effec

66 lyses demonstrate that SidJ modifies another Legionella effector SdeA, an unconventional phosphoribos

67 idE enzymes share the genetic locus with the Legionella effector SidJ that spatiotemporally opposes t

68 n a process that can be inhibited by another Legionella effector, Lpg2149.

69 ism of glutamylation via a pseudokinase-like Legionella effector, SidJ, in an ATP- and calmodulin-dep

70 and that the innate immunity kinase TAK1 and Legionella effectors compete to regulate Rab1 by switch

71 Among 302 Legionella effectors tested, 28 effectors affected TBSV

72 uding a subset of previously uncharacterized Legionella effectors that appear to be able to regulate

73 ing and delivery of two essential classes of Legionella effectors, depending on IcmSW or DotM, respec

74 tion pathway, here we show that 2 paralogous Legionella effectors, Lpg2154 (DupA; deubiquitinase for

75 Among these Legionella effectors, WipA has been primarily studied be

76 Thus, Legionella employs multiple sophisticated molecular mech

77 e show that three of these effectors [LegC2 (Legionella eukaryotic-like gene C2)/YlfB (yeast lethal f

78 Here we show that the Legionella export apparatus is localized to the bacteria

79 n pathogen, we have functionally analyzed 80 Legionella genomes spanning 58 species.

80 Gram-negative bacteria from the Legionella genus are intracellular pathogens that cause

81 gionella and biofilm formation, but promoted Legionella growth in pipes subject to convective mixing.

82 g copper-silver ionization for prevention of Legionella growth in water.

83 than Detroit water and exceeded the minimum Legionella growth temperature of 20 degrees C more frequ

84 regarding the variable effects of copper on Legionella growth, and confirms prior reports of trade-o

85 ver water that were potentially conducive to Legionella growth.

86 and silver concentrations were tested showed Legionella growth.

87 egionella-specific culture, all but 2 showed Legionella growth; 11 isolates were identical to 3 clini

88 e and demonstrate the intricate control that Legionella has over this unusual Ub-dependent posttransl

89 the impact of monochloramine disinfection on Legionella, heterotrophic bacteria (36 degrees C), Pseud

90 itinated species in host cells infected with Legionella In addition, we have identified a list of spe

91 ads to a substantial defect in the growth of Legionella in both its natural hosts (amoebae) and in mo

92 e than 180 PR-ubiquitinated host proteins in Legionella-infected cells.

93 Nevertheless, Legionella-infected macrophages induce an interleukin-1

94 elucidates how Legionella-infected macrophages use the alveolar epithel

95 ation was consistent with proven or probable Legionella infection in 84% of the cases.

96 and the control of energy metabolism during Legionella infection.

97 ights into host-pathogen interactions during Legionella infection.

98 l role in response to iron limitation during Legionella infection.

99 ety for Clinical Microbiology Study Group on Legionella Infections (ESGLI), were tested together with

100 clinical features of 33 consecutive cases of Legionella infections that occurred at the University of

101 we estimated the percentages of cases due to Legionella, influenza, and respiratory syncytial virus (

102 P&I hospitalizations could be attributed to Legionella, influenza, and RSV, respectively.

103 Legionella is a ubiquitous pathogen yet the global occur

104 om advanced stage (IIIB, IV) patients, while Legionella is higher in patients who develop metastases.

105 tal A-associated cases; and d) wgMLST showed Legionella isolates from cases exposed to hospital A and

106 In summary, diverse Legionella LD-causing species share a conserved core-gen

107 tive aerobic bacteria belonging to the genus Legionella; Legionella pneumophila serogroup 1 is the ca

108 Legionella longbeachae (Llo) and Legionella pneumophila

109 Here, we purify an intact T4CC from the Legionella membrane.

110 Here, we show that the Legionella meta-effector SidJ adopts a protein kinase fo

111 testing, releasing more iron, which is a key Legionella nutrient, while also directly causing disinfe

112 ng an overall estimate of seroprevalence for Legionella of 13.7% (95% CI 11.3-16.5), but with substan

113 viability haRPA, is able to identify viable Legionella on DNA microarrays.

114 ide experimental evidence for the model that Legionella pathogenesis in humans results from the cumul

115 work on the function of this effector during Legionella pathogenesis.

116 Legionella pathogenicity is mediated by specific virulen

117 ormation exists regarding effectors in other Legionella pathogens.

118 zed in the United States with a diagnosis of Legionella pneumonia in the Premier Perspectives databas

119 Two proven nosocomial cases of Legionella pneumonia occurred at the Wesley Hospital (Br

120 alone or a quinolone alone for treatment of Legionella pneumonia was associated with similar hospita

121 Legionella pneumonia was diagnosed in 3152 adults across

122 n or a quinolone antibiotic for treatment of Legionella pneumonia.

123 rsistence and associated risks of pathogenic Legionella pneumophila (L. pneumophila), thus raising hu

124 Legionella pneumophila (L.p.), the microbe responsible f

125 model for Mtb), Pseudomonas aeruginosa (Pa), Legionella pneumophila (Lp), and Enterococcus faecalis (

126 (UATs) for Streptococcus pneumoniae (SP) and Legionella pneumophila (LP).

127 Legionella longbeachae (Llo) and Legionella pneumophila (Lpn) are the most common pneumon

128 The enzymes DrrA/SidM and AnkX from Legionella pneumophila AMPylate and phosphocholinate Rab

129 ases in the intracellular bacterial pathogen Legionella pneumophila and identified the type 4 secreti

130 ay is necessary for thiamine biosynthesis in Legionella pneumophila and provide biochemical data to e

131 l role in forming the replication vacuole of Legionella pneumophila bacteria, which requires ERGIC-de

132 at a mono-ADP-ribosyltransferase, SdeA, from Legionella pneumophila catalyzes ADP-ribosylation of ubi

133 Legionella pneumophila causes a potentially fatal form o

134 Legionella pneumophila causes Legionnaires' disease, a s

135 Legionella pneumophila causes life-threatening pneumonia

136 The quantification of RNA from Legionella pneumophila cellular lysates was successfully

137 The gram-negative bacterial pathogen Legionella pneumophila creates a novel organelle inside

138 The bacterial pathogen Legionella pneumophila creates an intracellular niche pe

139 Importantly, Legionella spp. and Legionella pneumophila decreased after switching back to

140 The Legionella pneumophila Dot/Icm T4SS injects approximatel

141 The Legionella pneumophila Dot/Icm type IVB secretion system

142 The Legionella pneumophila effector MavC induces ubiquitinat

143 Here, we report the crystal structure of the Legionella pneumophila effector protein, SidJ, in comple

144 -ATPase through its interaction with SidK, a Legionella pneumophila effector protein.

145 The Legionella pneumophila effector vacuolar protein sorting

146 Therefore, we screened the Legionella pneumophila effectors to probe virus-host int

147 biquitination mediated by the SidE family of Legionella pneumophila effectors, such as SdeA, that cat

148 Legionella pneumophila encodes a family of phosphoribosy

149 The intracellular pathogen Legionella pneumophila encodes RidL to hijack the host s

150 Using transposon sequencing, we identify Legionella pneumophila genes required for growth in four

151 el of isolates of the opportunistic pathogen Legionella pneumophila GWAS revealed that the absence of

152 er domain of a guanidine III riboswitch from Legionella pneumophila has a different effect, stabilizi

153 irulence factors from the bacterial pathogen Legionella pneumophila has been discovered to modify hum

154 Legionella pneumophila has co-evolved with amoebae, thei

155 igation of legionellosis outbreaks caused by Legionella pneumophila However, as common sequence types

156 le and non-viable Legionella spp. as well as Legionella pneumophila in one hour.

157 at2, effectively suppress the replication of Legionella pneumophila in primary murine macrophages.

158 es intracellular infection of macrophages by Legionella pneumophila In the present study, we identifi

159 s well as proof-of-concept measurements with Legionella pneumophila including cell cultivation and pl

160 Legionella pneumophila infection blocked xenophagic targ

161 s of inflammation induced by oral cancer and Legionella pneumophila infection.

162 Legionella pneumophila infects human alveolar macrophage

163 The intracellular bacterium Legionella pneumophila inhibits host translation, thereb

164 The intracellular pathogen Legionella pneumophila interferes with autophagy by deli

165 Legionella pneumophila is a bacterial pathogen that thri

166 Legionella pneumophila is a bacterial pathogen that util

167 AnkB effector of the intravacuolar pathogen Legionella pneumophila is a bona fide F-box protein, whi

168 Legionella pneumophila is a causative agent of a severe

169 Legionella pneumophila is a causative agent of severe pn

170 Legionella comprises 65 species, among which Legionella pneumophila is a human pathogen causing sever

171 Legionella pneumophila is an environmental bacterium and

172 Legionella pneumophila is an intracellular bacterial pat

173 Legionella pneumophila is an intravacuolar pathogen that

174 Legionella pneumophila is an opportunistic pathogen that

175 evious studies established that the pathogen Legionella pneumophila is capable of hijacking Rab1 func

176 Legionella pneumophila is the causative agent of a sever

177 Legionella pneumophila is the causative agent of the sev

178 le genome sequence analysis was performed on Legionella pneumophila isolates from the infected patien

179 Previously, we reported that mutants of Legionella pneumophila lacking a type II secretion (T2S)

180 During infection, the bacterial pathogen Legionella pneumophila manipulates a variety of host cel

181 The bacterial pathogen Legionella pneumophila modulates host immunity using eff

182 icrobial communities, the bacterial pathogen Legionella pneumophila must withstand competition from n

183 Intracellular growth of Legionella pneumophila occurs in a replication vacuole c

184 opportunists via a cultivation-based assay (Legionella pneumophila only) and quantitative PCR.

185 nnaires' disease in a clinical setting where Legionella pneumophila PCR had been introduced.

186 resses translation of the hupA mRNA, and the Legionella pneumophila protein RocC binds the RocR sRNA,

187 Infection by the human pathogen Legionella pneumophila relies on the translocation of ap

188 se-11 was dispensable for the restriction of Legionella pneumophila replication in macrophages and in

189 es of the host cell cycle are permissive for Legionella pneumophila replication, whereas S phase prov

190 Members of the SidE effector family from Legionella pneumophila represent a new paradigm in the u

191 The intracellular pathogen Legionella pneumophila resides in a vacuole that is ubiq

192 /phosphodiesterase 1, Escherichia coli RppH, Legionella pneumophila Sde and Homo sapiens NudT16 (HsNu

193 ion of macrophages, the pathogenic bacterium Legionella pneumophila secretes effector proteins that i

194 phages, the intracellular bacterial pathogen Legionella pneumophila secretes effector proteins that m

195 Because Legionella pneumophila serogroup 1 is responsible for >8

196 bacteria belonging to the genus Legionella; Legionella pneumophila serogroup 1 is the causative agen

197 Legionella pneumophila serogroup 1 isolates were culture

198 In this report, we found that members of the Legionella pneumophila SidE effector family harbor a DUB

199 m type IV secretion system (T4SS) of several Legionella pneumophila strains.

200 Legionella pneumophila survives and replicates inside ho

201 o independently identify relevant sources of Legionella pneumophila that likely resulted in the outbr

202 retion system (T2SS) promotes the ability of Legionella pneumophila to grow in coculture with amoebae

203 Here, we used the bacterial pathogen Legionella pneumophila to understand how the immune syst

204 During infection, Legionella pneumophila translocates over 300 effector pr

205 Legionella pneumophila uses a single homodimeric disulfi

206 Legionella pneumophila uses a type IVB secretion system

207 The bacterial pathogen Legionella pneumophila utilizes approximately 300 effect

208 Legionella pneumophila utilizes the Dot/Icm type IV tran

209 inic acid from the lipopolysaccharide of the Legionella pneumophila virulence factor.

210 Legionella pneumophila was found in the sedimentation po

211 the DNA uptake system in the human pathogen Legionella pneumophila We found that a repressor of this

212 ologs encoded by the Philadelphia isolate of Legionella pneumophila were toxic to yeast, and SidJ sup

213 e ubiquitination and promotes infectivity of Legionella pneumophila, a pathogenic bacteria that cause

214 Legionella pneumophila, an intracellular pathogen respon

215 Legionella pneumophila, an intracellular pathogen that c

216 We show here that Legionella pneumophila, an intravacuolar pathogen that r

217 ctivity toward B. cereus 11778, B. subtilis, Legionella pneumophila, and Salmonella Typhimurium has d

218 Many intracellular pathogens like Legionella pneumophila, Coxiella burnetii, Listeria mono

219 Legionella pneumophila, Mycobacterium avium, and Pseudom

220 tive for at least one OPPP (Legionella spp., Legionella pneumophila, Mycobacterium avium, Mycobacteri

221 atypical and anaerobic pathogens, including Legionella pneumophila, Mycoplasma spp., Ureaplasma spp.

222 mon lung pathogens Streptococcus pneumoniae, Legionella pneumophila, or Mycobacterium tuberculosis-in

223 lytes of three pathogenic bacterial strains: Legionella pneumophila, Pseudomonas aeruginosa, and Salm

224 ography and sub-tomogram averaging of intact Legionella pneumophila, Pseudomonas aeruginosa, and Shew

225 uctures of three Gammaproteobacteria motors: Legionella pneumophila, Pseudomonas aeruginosa, and Shew

226 is, Escherichia coli, Klebsiella pneumoniae, Legionella pneumophila, Pseudomonas aeruginosa, Stenotro

227 Many bacteria, including Legionella pneumophila, rely on the type IV secretion sy

228 erologous expression of homologous lbtA from Legionella pneumophila, required for rhizoferrin biosynt

229 ) and 26GUmicroL(-1) for Legionella spp. and Legionella pneumophila, respectively, were achieved.

230 oazide (PMA) to simultaneously detect viable Legionella pneumophila, Salmonella typhimurium, and Stap

231 The intracellular pathogen, Legionella pneumophila, secretes approximately 300 effec

232 Proteases from Mycoplasma hyorhinis, Legionella pneumophila, Streptococcus pneumonia and Cand

233 Legionella pneumophila, the agent of Legionnaires' disea

234 For Legionella pneumophila, the bacterium translocates prote

235 Legionella pneumophila, the causative agent of Legionnai

236 Legionella pneumophila, the causative agent of Legionnai

237 is a previously uncharacterized effector of Legionella pneumophila, the causative agent of Legionnai

238 mune response to bacterial pathogens such as Legionella pneumophila, the causative agent of Legionnai

239 The facultative intracellular pathogen Legionella pneumophila, the causative agent of Legionnai

240 Legionella pneumophila, the causative agent of Legionnai

241 Legionella pneumophila, the etiological agent of Legionn

242 Legionella pneumophila, the etiological agent of Legionn

243 Legionella pneumophila, the Gram-negative pathogen causi

244 scovery protocol by targeting this enzyme in Legionella pneumophila, the major causative agent of Leg

245 Legionella pneumophila, the most commonly identified cau

246 Legionella pneumophila, the primary agent of Legionnaire

247 In Legionella pneumophila, the two-component system (TCS) P

248 ddition, during coinfection experiments with Legionella pneumophila, we found that defective intracel

249 These findings contrasted with those for Legionella pneumophila, where chemical inhibition of the

250 intact T2SS imaged within the human pathogen Legionella pneumophila.

251 on with the intracellular bacterial pathogen Legionella pneumophila.

252 re form of pneumonia caused by the bacterium Legionella pneumophila.

253 se (Thi5) was necessary for HMP synthesis in Legionella pneumophila.

254 e ubiquitination and promotes infectivity of Legionella pneumophila.

255 racellular iron acquisition strategy used by Legionella pneumophila.

256 ubsequent infection with Escherichia coli or Legionella pneumophila.

257 ella burnetii, Agrobacterium tumefaciens and Legionella pneumophila.

258 ingent response nor the previously described Legionella quorum-sensing pathway.

259 ligase activity, is required for successful Legionella replication in a viable eukaryotic host cell.

260 re formation, pyroptosis, and restriction of Legionella replication in macrophages and in vivo.

261 he NLRC4 inflammasome and the restriction of Legionella replication in macrophages and in vivo.

262 related to the NLRC4-mediated restriction of Legionella replication were performed using mice double

263 review provides an overview of reservoirs of Legionella reported in the literature, other than drinki

264 ination of hot water distribution systems by Legionella represents a great challenge due to difficult

265 No genomic microevolution and no Legionella resistance to antibiotics were detected.

266 Two cases were documented through positive Legionella RT PCR at 52 and 53 days (cycle threshold det

267 ion of substrates is critically required for Legionella's alteration of the host endocytic pathway, a

268 fferent proportions of viable and non-viable Legionella, shown with the example of L. pneumophila, ra

269 cases of pneumonia due to L. donaldsonii and Legionella sp. D5382 are likely the first reports of hum

270 hernii, L. parisiensis, L. sainthelensi, and Legionella sp. strain D5382.

271 p serogroups 1-17 and 17 emergent LD-causing Legionella species (of which 33 were sequenced in this s

272 ssembled and characterized the genomes of 38 Legionella species and predicted their effector repertoi

273 Together, these results suggest that diverse Legionella species infect patients with cancer in the Ho

274 Legionella species inhabit freshwater and soil ecosystem

275 exposure, sex, detection methods, IFA titre, Legionella species measured, and present seroprevalence

276 The 33 strains involved 12 Legionella species or subspecies: 15 strains of L. pneum

277 eumophila can antagonize the growth of other Legionella species using a secreted inhibitor: HGA (homo

278 The effector repertoires of different Legionella species were found to be largely non-overlapp

279 enome composition and host range of multiple Legionella species, we demonstrate that their distinct e

280 -negative bacteria, Mycobacterium species or Legionella species.

281 splant, with their infections caused by five Legionella species.

282 und in diverse conjugative elements in other Legionella species.

283 here environmental samples were obtained for Legionella-specific culture, all but 2 showed Legionella

284 Importantly, Legionella spp. and Legionella pneumophila decreased aft

285 units (GU) microL(-1) and 26GUmicroL(-1) for Legionella spp. and Legionella pneumophila, respectively

286 at is able to quantify viable and non-viable Legionella spp. as well as Legionella pneumophila in one

287 eated rainwater, microbial proliferation and Legionella spp. gene copy numbers were often three logs

288 gger flagellin/NLRC4-mediated restriction of Legionella spp. infection in macrophages and in vivo.

289 Legionella spp. is a key contributor to the United State

290 Recovery values for viable Legionella spp. were found between 81% and 133%.

291 Legionella spp., another genus containing potential oppo

292 events were positive for at least one OPPP (Legionella spp., Legionella pneumophila, Mycobacterium a

293 However, concentrations of Legionella spp., M. intracellulare, Acanthamoeba spp., a

294 umophila were not detected by either method; Legionella spp., nontuberculous mycobacteria (NTM), and

295 a high correlation coefficient (R=0.994) for Legionella spp., with a detection limit of 0.1 ng of the

296 The Legionella strains were isolated from bronchoscopy speci

297 e found nearly exclusively in eukaryotes and Legionella Translocation assays for selected Rab-GTPase

298 ds at the Cleveland Clinic were searched for Legionella urinary antigen (UAG), culture, and PCR tests

299 ecular mechanisms of an effector involved in Legionella virulence and may inform approaches to elucid

300 e, crucial for elucidating the mechanisms of Legionella virulence.

301 plication, the identified DrrA effector from Legionella was further exploited.