ライフサイエンスコーパス: Limulus

1 ve given rise to the appendage morphology of Limulus.

2 nt hemolytic activity found in the plasma of Limulus.

3 he ventral optic nerve of the horseshoe crab Limulus.

4 sea cucumber Thyone, and the horseshoe crab Limulus.

5 its toxicity 20,000-fold, as assayed in the Limulus amebocyte lysate (LAL) test.

6 crofluidic automation and parallelization of Limulus amebocyte lysate (LAL)-based bacterial endotoxin

7 ramatically reduces its ability to coagulate Limulus amebocyte lysate (LAL).

8 Both Limulus amebocyte lysate activity and nitric oxide produ

9 tudy, neither L-37pA nor D-37pA affected the Limulus amebocyte lysate activity of LPS, indicating tha

10 S, as assessed by LPS-induced coagulation of limulus amebocyte lysate and production of tumor necrosi

11 LF-33 inhibited the coagulation of the Limulus amebocyte lysate and the secretion of TNF-alpha

12 d its biological activity examined using the Limulus amebocyte lysate assay and nitric oxide producti

13 We evaluated levels of plasma LPS (by the Limulus amebocyte lysate assay) and immune activation ma

14 The presence of endotoxin (detected by the Limulus amebocyte lysate assay) was compared to the pres

15 chest pain were analyzed for endotoxin/LPS (Limulus amebocyte lysate assay), lipid profile, troponin

16 platelet-rich plasma were detected using the limulus amebocyte lysate assay, according to differentia

17 ent is heat stable, reacts positively in the Limulus amebocyte lysate assay, and can be inhibited by

18 ins, which is difficult to estimate with the Limulus amebocyte lysate assay, and cannot be completely

19 oxin was measured by a kinetic turbidimetric limulus amebocyte lysate assay, and serum NO metabolite

20 t assay, and lipopolysaccharide (LPS) by the limulus amebocyte lysate assay, at presentation and afte

21 the mutant showed decreased toxicity by the Limulus amebocyte lysate assay, reduced adherence to hum

22 owed significantly decreased toxicity by the Limulus amebocyte lysate assay, reduced resistance to no

23 analysis of LPS bioactivity, using modified limulus amebocyte lysate assay, suggest that recombinant

24 ficant endotoxin activity as detected by the Limulus amebocyte lysate assay.

25 ndotoxin levels were determined by using the limulus amebocyte lysate assay.

26 yxin B, displayed only minor activity in the Limulus amebocyte lysate assay.

27 ed for endotoxin level determination is LAL (Limulus Amebocyte Lysate) assay.

28 , cleaned and tested for endotoxin using the limulus amebocyte lystate (LAL) gel clot method.

29 n was measured using the kinetic chromogenic limulus amoebocyte lysate (LAL) assay.

30 s: ethylene diamine tetraacetic acid (EDTA); limulus amoebocyte lysate assay (LAL); pertussis toxin (

31 thod was validated against the gold standard Limulus Amoebocyte lysate assay in real bacteria culture

32 In a standard measure of LPS activity, the Limulus amoebocyte lysate assay, there was approximately

33 ich were too low to be detected by using the Limulus amoebocyte lysate assay.

34 of the original free LPSs as measured by the Limulus amoebocyte lysate assay.

35 from the parent strain as measured by both a Limulus amoebocyte lysate endotoxin quantitation assay a

36 The LPS lacks any limulus amoebocyte lysate gelation activity.

37 Endotoxicity, measured by Limulus amoebocyte lysate kinetic assay, showed that the

38 ual Detergent or Enzol Detergent, or sterile limulus amoebocyte lysate reagent water as a control.

39 pendent manner in the in vitro reaction with Limulus amoebocyte lysate.

40 the junction of the respiratory book gill of Limulus and differentiates it from the typically robust

41 Limulus antilipopolysaccharide factor (LALF) can neutral

42 , a recombinant peptide that is derived from Limulus antilipopolysaccharide factor and targets lipid

43 , and the bulbous claspers in Tachypleus and Limulus are derived.

44 We used a chromogenic Limulus assay to determine the EC50 of the peptide (the

45 high-fat meal with 3 cigarettes by using the limulus assay.

46 Invertebrates such as Drosophila or Limulus assemble their visual pigment into the specializ

47 ytilus) or five to six times initial length (Limulus), at forces approximately 18 nN and approximatel

48 form can be homologized with those of living Limulus, but retain an ancestral biramous morphology.

49 c acid-binding lectins of Limulus plasma and Limulus C-reactive protein were nonhemolytic.

50 e, which binds limulin and separates it from Limulus C-reactive protein, the most abundant pentraxin

51 nd is present at a much lower abundance than Limulus C-reactive protein, the other plasma pentraxin.

52 erated two probes that were used to search a Limulus cDNA library produced from central nervous syste

53 quently, two cDNA libraries derived from the Limulus central nervous system (CNS) were screened and f

54 Hydropathy plot analysis predicts the Limulus choline co-transporter (LChCoT) to have thirteen

55 , lipoxygenase, alpha toxin (LH2/PLAT); (iv) Limulus clotting factor C, Coch-5b2 and Lgl1 (LCCL).

56 multiple adhesive modules including a common Limulus coagulation factor C domain also found in two ad

57 us occurred before both the emergence of the Limulus CRP variants and the mammalian CRP/SAP gene dupl

58 uctural and functional changes that occur in Limulus eyes in response to clock input.

59 id residues of cochlin, corresponding to the Limulus factor C-homology (cochFCH) domain; this domain

60 The full length nucleotide sequence of the Limulus homolog consists of 3368 bp which includes an op

61 gated the biosynthesis of rhodopsin from the Limulus lateral eye with three cell culture expression s

62 ted in the endogenous protein extracted from Limulus lateral eye, and that enhanced phosphorylation a

63 er, injecting Xenopus oocytes with mRNA from Limulus lateral eyes yielded light-dependent conductance

64 Intriguingly, limulus lectin L6, a structurally related antibacterial

65 Here we describe an ENU-induced mutation, limulus (lulu), which disrupts gastrulation and the orga

66 ) was assayed at 0, 15, and 30 minutes using limulus lysate assay (LAL) and EndoCAb Ig assays.

67 A limulus lysate assay was used to quantify beta -1,3 gluc

68 ommunication describes a modification of the Limulus lysate assay which allows precise quantitation o

69 We measured LPS levels by use of limulus lysate assay, and DNA sequences encoding bacteri

70 ion of myosin filaments from horseshoe crab (Limulus) muscle.

71 ined the sites that become phosphorylated in Limulus myosin III and investigated its kinase, actin bi

72 We seek to understand the role of Limulus myosin III and its phosphorylation in photorecep

73 peculate that interactions between actin and Limulus myosin III are regulated by both second messenge

74 Finally, we demonstrate that Limulus myosin III binds actin but lacks ATPase activity

75 We show that Limulus myosin III exhibits kinase activity and that a m

76 es within the actin interface of full-length Limulus myosin III expressed in baculovirus are substrat

77 We conclude that Limulus myosin III is an actin-binding and signaling pro

78 We propose that the phosphorylation of Limulus myosin III is involved in one or more of the str

79 Limulus myosin III is particularly interesting because i

80 We report that Limulus myosin III is similar to other unconventional my

81 We show that the kinase domain of Limulus myosin III shares some pharmacological propertie

82 hosomal somites 4 and 5; although present in Limulus, no other arachnids have opisthosomal appendage

83 e that the CRP/SAP gene duplication event in Limulus occurred before both the emergence of the Limulu

84 Also, expressing Limulus opsin cDNA in the R1-R6 photoreceptors of transg

85 Similarly, Xenopus oocytes injected with Limulus opsin cRNA did not evoke light-sensitive current

86 These results indicate that Limulus opsin may require one or more photoreceptor-spec

87 We developed antibodies directed against Limulus opsin, visual arrestin, and myosin III, and we h

88 because of a lack of antibodies specific for Limulus photoreceptor proteins.

89 Phototransduction in Limulus photoreceptors involves a G protein-mediated act

90 to examine the distribution of calmodulin in Limulus photoreceptors.

91 ne of several sialic acid-binding lectins of Limulus plasma and is present at a much lower abundance

92 The other sialic acid-binding lectins of Limulus plasma and Limulus C-reactive protein were nonhe

93 PNA), Maclura pomifera agglutinin (MPA), and Limulus polyphemus agglutinin (LPA), suggesting the pres

94 ancient "living fossil", the horseshoe crab Limulus polyphemus and determined the three-dimensional

95 orseshoe crabs-Carcinoscorpius rotundicauda, Limulus polyphemus and Tachypleus tridentatus.

96 The photoreceptors of the horseshoe crab Limulus polyphemus are classical preparations for studie

97 However, the fertilization of a Limulus polyphemus egg involves a third type of actin-ba

98 of Mytilus and the telson-levator muscle of Limulus polyphemus have shown large, reversible length c

99 rum amyloid P component (SAP)-like pentraxin Limulus polyphemus SAP is a recently discovered, distinc

100 During the 5 s of the acrosome reaction of Limulus polyphemus sperm, a 60-microm-long bundle of scr

101 The lateral eyes of the horseshoe crab Limulus polyphemus undergo dramatic daily changes in str

102 e kinase in the phototransduction cascade in Limulus polyphemus ventral photoreceptors.

103 um release and of the electrical response in Limulus polyphemus ventral photoreceptors.

104 ng 22-46 years for Atlantic horseshoe crabs (Limulus polyphemus) in the Long Island Sound, USA region

105 process of the sperm of the horseshoe crab (Limulus polyphemus) is a unique crystalline actin bundle

106 he acrosomal reaction of the horseshoe crab (Limulus polyphemus) sperm.

107 hagfish (Myxine glutinosa), horseshoe crab (Limulus polyphemus), and cone snail (Conus textile) to c

108 erum-amyloid-P-component-like pentraxin from Limulus polyphemus, a recently discovered pentraxin spec

109 The chelicerate Limulus polyphemus, all isopod crustaceans tested, and t

110 ssess gene arrangements identical to that of Limulus polyphemus, and P. opilio is found to have a sim

111 In the horseshoe crab Limulus polyphemus, enhanced phosphorylation of an abund

112 ynchus keta, Ilyanassa obsoleta, Bos taurus, Limulus polyphemus, Saccharyomyces cerevisiae).

113 telson levator muscle of the horseshoe crab Limulus polyphemus.

114 polysaccharide-binding coagulation factor in Limulus polyphemus.

115 phism from populations of the horseshoe crab Limulus polyphemus.

116 rgan, the lateral eye of the horseshoe crab, Limulus polyphemus.

117 n the plasma of the American horseshoe crab, Limulus polyphemus.

118 n chelicerates, including the horseshoe crab Limulus polyphemus.

119 st contiguous fragments with the chelicerate Limulus polyphemus.

120 holine co-transporter in the horseshoe crab, Limulus polyphemus.

121 Drosophila melanogaster and MyoIII(Lim) from Limulus polyphemus.

122 at both prototypic pentraxins are present in Limulus raises the possibility that both were present in

123 serve a similar backbone organization in the Limulus reconstruction, supporting the general existence

124 tracted and affinity-purified epitope-tagged Limulus rhodopsin expressed from a cDNA or cRNA from the

125 Photoisomerization of Limulus rhodopsin leads to phosphoinositide hydrolysis,

126 Limulus SAP is synthesised as a precursor protein of 234

127 Phylogenetic analysis clusters Limulus SAP pentraxin with the horseshoe crab C-reactive

128 The complete cDNA sequence of Limulus SAP, and the derived amino acid sequence, the fi

129 Limulus SAP, which does not exhibit the CRP characterist

130 The structure of the previously undiscovered Limulus serum amyloid P component, the first invertebrat

131 During activation of the Limulus sperm acrosomal process, actin filaments undergo

132 Limulus sperm contains a dynamic macromolecular structur

133 The acrosomal bundle in the Limulus sperm has been shown to be a quasi-crystalline a

134 econstruction of an actin-scruin bundle from Limulus sperm reveals details about the enormous structu

135 In the unactivated Limulus sperm, a 60- micro m-long bundle of actin filame

136 In the acrosomal process of Limulus sperm, the beta-propeller protein scruin cross-l

137 fferent regions of the sequence in human and Limulus structures, occupy similar space within the over

138 Here, the structure of Limulus substrate-free AK is refined against high-resolu

139 eractions seen in tarantula are also seen in Limulus, supporting the hypothesis.

140 eened and four distinct isoforms, designated Limulus syntaxin (Lim-syn) 1A, 1B, 1C and 1D, were obtai

141 erein the cloning of a group of syntaxins in Limulus that are associated with the plasma membrane.

142 itol 1,4,5-trisphosphate (Ins 1,4,5-P3) into Limulus ventral photoreceptors produces excitation simil

143 bearing photosensitive membrane (rhabdom) in Limulus ventral photoreceptors.

144 Thus, the Limulus visual system may be particularly useful for inv

145 e the distributions of these proteins in the Limulus visual system.

146 ial length (Mytilus) and 66% initial length (Limulus) were fully reversible and took place within the

147 +), resulting in photoreceptor excitation in Limulus, while DAG may activate PKC.