ライフサイエンスコーパス: NGS

1 NGS allowed us to characterize the local phylodynamics o

2 NGS analysis identified 40 upregulated and 40 downregula

3 NGS can also be used in a standard diagnostic pipeline t

4 NGS data may help to identify single nucleotide variants

5 NGS estimates of sequences at nucleotide 2493 with known

6 NGS has been applied to understand genomic, transcriptom

7 NGS provides a significant statistical base that enables

8 NGS revealed changes in expression levels of EMT markers

9 NGS revealed several prominent mutations in the nonstruc

10 NGS studies have uncovered an ever-growing catalog of hu

11 NGS uncovered a high prevalence of PDR among participant

12 NGS Wnt supports long-term expansion of multiple differe

13 NGS Wnts are superior to Wnt3a conditioned media in orga

14 NGS-based serology via SERA provides an effective approa

15 NGS-derived average pairwise diversity classified an inf

16 expanded with the powerful coupling of HIV-1 NGS data with epidemiological data.

17 A input requirement (Swift Biosciences Accel-NGS, Illumina TruSeq and QIAGEN QIAseq) on two state-of-

18 However, the laboratories adopting NGS methods face significant challenges due to the compl

19 Target capture and amplicon NGS demonstrated a high coverage rate of HTNV in Apodemu

20 Furthermore, the amplicon NGS showed a 10-fold (10(2) copies/muL) higher sensitivi

21 An NGS approach performed on maternal DNA from both periphe

22 the size distribution of DNA fragments in an NGS library, or other DNA sample, based on gel-electroph

23 llenges and considerations for setting up an NGS and bioinformatics-focused infectious disease resear

24 AR uses an NGS-derived breakpoint graph alongside OM scaffolds to p

25 onsidered in the interpretation of array and NGS-based genomic data for precision medicine.

26 read sequencing from Pacific Biosciences and NGS barcoding from 10x Genomics hold the potential for f

27 tational screening of EYA4 gene by DHLPC and NGS in a large cohort of 531 unrelated Spanish probands

28 both KIT D816V variant allele frequency and NGS panels) to lend more diagnostic and prognostic clari

29 elation between %472-C results by MAPREC and NGS was observed in all laboratories (Pearson correlatio

30 ed to analyze high-throughput microarray and NGS data from large publicly-available repositories.

31 ranscriptase quantitative PCR (RT-qPCR), and NGS.

32 therapy were analyzed in parallel by SS and NGS in 1 of 4 reference laboratories.

33 3 blood samples and 504 publically available NGS datasets.

34 t-generation sequencing (NGS), and RNA-based NGS.

35 by SS had low-level mutations detectable by NGS.

36 One biopanning round followed by NGS selects robust PP-binding peptides that are not evid

37 Mutations that were found by NGS were analyzed in vitro.

38 ype's amplicon copies prior to genotyping by NGS, thereby maximizing multiple-type sensitivity with m

39 uctural variant (SV) analyses are limited by NGS read lengths.

40 tated IGHV were more likely to have U-MRD by NGS at the end of treatment (EOT; 41% vs 13%, P = .02) t

41 No patient with U-MRD by NGS in BM or PBMC was MRD+ in plasma.

42 High-throughput screens powered by NGS have greatly increased the rate of variant functiona

43 wever, the amount of information produced by NGS has made it difficult for researchers to choose the

44 mosacism and wide indel spectrum revealed by NGS and fragment analysis.

45 fresh frozen (FF) tissues were sequenced by NGS.

46 The bulk of space taken up by NGS sequencing CRAM files consists of per-base quality v

47 were identified as low-abundance variants by NGS.

48 ) higher sensitivity than the target capture NGS.

49 efficient compression software for clinical NGS data goes beyond the computational interest; as it u

50 menting accurate variant calling in clinical NGS testing.

51 d variant persistence identified on clinical NGS panels, despite a change in disease state.

52 eport provides details on how some clustered NGS form microdomains that can be identified and tracked

53 In this review, we summarize the most common NGS and bioinformatics workflows in the context of infec

54 of reproducibility and costs in the complete NGS workflow.

55 Comprehensive NGS analysis reliably detects relevant mutations, amplif

56 combinations, including the highly conserved NGS N262, N448, and N301, created an immune escape map o

57 ntroduced at various steps of a conventional NGS workflow, such as sample handling, library preparati

58 of sequencing error sources in conventional NGS workflows.

59 ncing, an extremely accurate error-corrected NGS (ecNGS) technology, we were able to detect mutations

60 facilitate barrier-free learning of current NGS techniques and provides a user-friendly interface fo

61 uggest a 'fit-for-purpose' triage of current NGS technologies.

62 o recently established diagnostic tools (DNA NGS and/or EQUC).

63 veSEQ-HIV, a high-throughput, cost-effective NGS sequencing method and computational pipeline tailore

64 Most existing NGS data analysis tools focus on the microRNA component

65 th the same serum exosomes processed for EXO-NGS, we observed two long non-coding RNAs, malat-1 and C

66 NA cargos by next generation sequencing (EXO-NGS).

67 fferential mRNA expression observed upon EXO-NGS analysis, we independently evaluated two protein cod

68 Here we compared the performance of five NGS HIVDR pipelines using proficiency panel samples from

69 FLASH-NGS achieves up to 5 orders of magnitude of enrichment a

70 ified by a statistical analysis adjusted for NGS read count and fetal fraction.

71 In multivariable analysis for NGS-positive patients, adjusting for disease risk and do

72 ion and maintenance of quality practices for NGS workflows.

73 ght the need for standardized strategies for NGS HIVDR data analysis, especially for the detection of

74 sent a microfluidic droplet-based system for NGS library preparation, capable of reducing the number

75 Tumor cells used for NGS may contain germline, somatic, and clonal hematopoie

76 sues of 139 patients with CRLM were used for NGS.

77 s, we recommend HG38 (the newer version) for NGS SNV analysis.

78 iated variants not currently accessible from NGS.

79 In silico secondary structure analyses from NGS indicated extensive TAR stem-loop malformations pred

80 cing (NGS) technologies, data generated from NGS is being used for pathogen detection in cancer.

81 entially new Papillomaviridae sequences from NGS amplicon sequencing with degenerate PV primers.

82 ransforms NCBI Gene Expression Omnibus (GEO) NGS content into deeply integrated gene expression and c

83 powered by these technologies and highlights NGS approaches that hold particular promise for generati

84 tional laboratories using their own in-house NGS assays.

85 lyzed by 8 laboratories using their in-house NGS method.

86 ng could reduce the burden for completing ID-NGS clinical trials.

87 rt of a composite validation strategy for ID-NGS diagnostic tests.

88 A pivotal problem in NGS based small RNA analysis is identifying and quantify

89 known or novel expanded repeat sequences in NGS data.

90 -specific uridine to cytidine transitions in NGS data.

91 ches to search for known or novel viruses in NGS data, no appropriate tool is available for the class

92 For instance, NGS and bioinformatics approaches have been used to iden

93 empirically characterized the interdependent NGS of a microdomain in the high-mannose patch (HMP).

94 By leveraging NGS sample multiplexing capabilities, the per-sample lab

95 Many NGS HIVDR data analysis pipelines have been independentl

96 Among these 58 infections, metagenomic NGS identified 13 (22%) that were not identified by clin

97 e investigated the usefulness of metagenomic NGS of CSF for the diagnosis of infectious meningitis an

98 In this study, metagenomic NGS of CSF obtained from patients with meningitis or enc

99 ata exist on its use in vitro Moreover, most NGS studies do not separate integrated from unintegrated

100 han the reference genome; and applying novel NGS techniques to improve the sensitivity of amplicon-ba

101 Technical error rates (~1 x 10(-3)) of NGS obscure the true abundance of somatic mutations, whi

102 ows have been developed to take advantage of NGS and have placed the resulting immunome datasets in t

103 However, the storage of large amounts of NGS data and visualization tools need to evolve to offer

104 by providing a solution for the analysis of NGS amplicon sequencing, increasingly used in clinical r

105 Analysis of NGS data confirmed linkage for the 2 index-partner pairs

106 d an extensive framework for the analysis of NGS functionalization screens available as an R package

107 Our focus is on the application of NGS in the context of pathogen genomics.

108 The integrated application of NGS technologies to samples from carefully phenotyped cl

109 ritical steps in the clinical application of NGS-based genetic testing from an informatics perspectiv

110 The high consistency of NGS data demonstrates that NGS analysis can be used as h

111 Reducing the space consumption of NGS data reduces the cost of storage and data transfer.

112 The greater depth of NGS sequence information over traditional sequencing met

113 Research." We briefly discuss the history of NGS technologies and describe how the techniques develop

114 l mutations, supporting the incorporation of NGS-based BCR-ABL1 KD mutation screening results in the

115 visited to integrate the sequencing power of NGS.

116 age sites over a 1,000-fold dynamic range of NGS counts and produced consensus and optimal cleavage m

117 e the scoring system based on the results of NGS.

118 dividuals should give consent at the time of NGS testing to receive information about potential germl

119 e feasibility, cost, and turnaround times of NGS-based BCR-ABL1 mutation screening in a routine setti

120 licable to the analysis of numerous types of NGS assays and experimental designs.

121 While many limitations to the use of NGS in clinical microbiology laboratories are being over

122 The use of NGS to study reservoir dynamics in vitro provides a mode

123 boratory, and interlaboratory variability of NGS 472-C estimates across samples and laboratories were

124 Based on NGS-derived data, we identified 16 aptamer candidates.

125 The effects of DTF on NGS analysis were negligible.

126 rom the defined spectrum within a particular NGS dataset.

127 his study shows that institutions performing NGS sequencing for cancer genomics should incorporate th

128 In addition to dynamic repertoire profiles, NGS analysis reveals differential patterns of HEV-specif

129 However, implementation of high-quality NGS and bioinformatics in research and public health lab

130 Raw NGS data were then processed using each of the five diff

131 rs the benefit of using duplex UMI to remove NGS artifacts in a much more simplified workflow than ex

132 cations at a single time point, and repeated NGS testing allows tracking of dynamic changes in varian

133 standardized template for clinical reporting NGS results for Mycobacterium tuberculosis.

134 therapy-induced NED, we performed small RNA-NGS in a retrospective cohort of human metastatic CRPC c

135 diagnostic performance of plasma mcfDNA-Seq NGS in 114 HCT recipients with pneumonia after HCT who h

136 Next-generation DNA-sequencing (NGS) technologies, which are designed to streamline the

137 rRNA rapid next-generation gene sequencing (NGS) and expanded quantitative urine culture (EQUC).

138 Next-generation sequencing (NGS) allows detection of low-level kinase domain mutatio

139 Next generation sequencing (NGS) allows for scalable, genome-wide studies of small R

140 a substrate for next-generation sequencing (NGS) analysis and is likely to have wider applications f

141 Next-generation sequencing (NGS) analysis was compared to the current MAPREC (mutati

142 r antigens using next-generation sequencing (NGS) analysis.

143 istency with the Next Generation Sequencing (NGS) analysis.

144 ere subjected to next-generation sequencing (NGS) and 3 of 8 tissues yielded complete IAV pH1N1 genom

145 time PCR (qPCR), next-generation sequencing (NGS) and bioinformatic data analysis.

146 Next-generation sequencing (NGS) and bioinformatics processing were used for the ide

147 ne has exploited next-generation sequencing (NGS) and gene/immune-targeted drug deployment to transfo

148 nscriptome using Next Generation Sequencing (NGS) and identified a subset of sequences representing p

149 l conditions for next-generation sequencing (NGS) and instructions for data analysis.

150 were explored by next generation sequencing (NGS) and other mutant screening methods including T7 end

151 Next-generation sequencing (NGS) and related omics technologies, such as transcripto

152 urrent practice, Next Generation Sequencing (NGS) applications start with mapping/aligning short read

153 uccessful, but a next-generation sequencing (NGS) approach identified KARYV and KUNDV as viruses in t

154 d a whole-genome next-generation sequencing (NGS) approach to examine single nucleotide polymorphisms

155 We developed a next-generation sequencing (NGS) approach to identify drug-resistance mutations in e

156 nologies such as next-generation sequencing (NGS) are revolutionizing molecular diagnostics and clini

157 have developed a Next Generation Sequencing (NGS) assay, XACTLY, to interrogate the termini of fragme

158 NA-seq and other next-generation sequencing (NGS) assays that profile transcriptional activity.

159 studies utilize next generation sequencing (NGS) but until now the results of different experiments

160 Next-generation sequencing (NGS) can be used to quantify B cell repertoire response

161 Next-generation sequencing (NGS) can identify novel cancer targets.

162 capabilities of next-generation sequencing (NGS) can provide new insights into the degree of genetic

163 Next generation sequencing (NGS) combined with bioinformatics has successfully been

164 rovides adequate next-generation sequencing (NGS) data for downstream bioinformatics analysis.

165 IV-1 in DC using next-generation sequencing (NGS) data.

166 d length of most next-generation sequencing (NGS) datasets and are not profiled by existing genome-wi

167 As the use of next-generation sequencing (NGS) for the Mendelian diseases diagnosis is expanding,

168 custom-targeted next generation sequencing (NGS) gene panel was used to identify SLC38A8 mutations f

169 a broad utility next generation sequencing (NGS) gene panel.

170 RD evaluation by next-generation sequencing (NGS) has a sensitivity of 10-6 (MRD6).

171 Next-generation sequencing (NGS) has become a mainstream method in bioanalysis.

172 Next-generation sequencing (NGS) has been widely adopted to identify genetic variant

173 While next-generation sequencing (NGS) has dramatically increased the availability of geno

174 ation screening, next-generation sequencing (NGS) has recently been assessed in retrospective studies

175 Next-generation sequencing (NGS) has the potential to improve both clinical diagnost

176 Advances in next-generation sequencing (NGS) have made available a wealth of information that ha

177 Next-generation sequencing (NGS) identified the best discriminating ssODNs, nine of

178 d centrally with next-generation sequencing (NGS) in a master screening protocol.

179 ors (TCRs) using next-generation sequencing (NGS) in neonates, infants, and children can provide valu

180 Next Generation Sequencing (NGS) is a commonly used technology for studying the gene

181 Next-generation sequencing (NGS) is a conceptually attractive alternative for detect

182 Next-generation sequencing (NGS) is a potent method to sequence the viral genome, us

183 Next generation sequencing (NGS) is a trending new standard for genotypic HIV-1 drug

184 ng with Illumina next-generation sequencing (NGS) is applied to reveal insights into peptide-based ad

185 ecreasing costs, next-generation sequencing (NGS) is still prohibitively costly for routine use in ge

186 Next-generation sequencing (NGS) is widely used in genetic testing for the highly se

187 high-resolution next generation sequencing (NGS) method.

188 Next-generation sequencing (NGS) methods are deconstructing the genomic landscape of

189 Next-generation sequencing (NGS) of bone marrow and peripheral blood increasingly gu

190 Metagenomic next-generation sequencing (NGS) of cerebrospinal fluid (CSF) has the potential to i

191 Next-generation sequencing (NGS) of DNA and the falling cost of sequencing due to co

192 Next generation sequencing (NGS) of DNAs amplified by rolling circle amplification f

193 Next generation sequencing (NGS) of LTRs from 269 HIV-1-infected samples in the Drex

194 00 copies/mL had next-generation sequencing (NGS) of the HIV pol gene with MiSeq technology.

195 Next-generation sequencing (NGS) offers the opportunity for rapid, comprehensive DST

196 , we carried out next-generation sequencing (NGS) on B cell transcripts from donor N123, the source o

197 re, we performed next-generation sequencing (NGS) on plasma DNA with and without bisulfite treatment

198 ions analyzed by next-generation sequencing (NGS) on the oncological outcome after resection of color

199 2 patients using next-generation sequencing (NGS) panel analysis.

200 ation and use of next-generation sequencing (NGS) panels to profile the genetic landscape of SM varia

201 on capture-based next-generation sequencing (NGS) platform.

202 have developed a Next-Generation Sequencing (NGS) platform: viral Photo-Activatable Ribonucleoside Cr

203 ads generated by next-generation sequencing (NGS) platforms into biological knowledge.

204 Next-generation sequencing (NGS) represents a powerful tool to unravel the genetic m

205 d amplicon-based next-generation sequencing (NGS) should be used as the gold standard assay for asses

206 Next generation sequencing (NGS) showed that over 600 gRNAs including the ones targe

207 Next-generation sequencing (NGS) techniques are revolutionizing biomedical research

208 strains by using next-generation sequencing (NGS) techniques.

209 one of the major next generation sequencing (NGS) technologies and it is frequently used in medical r

210 Next Generation Sequencing (NGS) technologies are considered as the most powerful an

211 e development of next-generation sequencing (NGS) technologies has made it possible to identify large

212 The advent of Next-Generation Sequencing (NGS) technologies has opened new perspectives in deciphe

213 Next-generation sequencing (NGS) technologies have been widely used to screen for vi

214 Next-generation sequencing (NGS) technologies have changed the landscape of genetic

215 Next-generation sequencing (NGS) technologies have revolutionized multiple areas in

216 seases entitled "Next-Generation Sequencing (NGS) Technologies to Advance Global Infectious Disease R

217 th the advent of next generation sequencing (NGS) technologies, data generated from NGS is being used

218 The next generation sequencing (NGS) technology including whole exome sequencing (WES) a

219 ded) by powerful next-generation sequencing (NGS) technology, and copied numbers of the DNA codes rep

220 We used next-generation sequencing (NGS) to demonstrate that favipiravir causes mutations in

221 ge libraries and next-generation sequencing (NGS) to identify hydrophilic, net-neutral charged peptid

222 s (>150 kb) with next-generation sequencing (NGS) to resolve fCNAs at single-nucleotide resolution.

223 Here, we use next-generation sequencing (NGS) with barcoding of individual RNA molecules to accur

224 n PCR, DNA-based next-generation sequencing (NGS), and RNA-based NGS.

225 er sequencing or next-generation sequencing (NGS), providing high-throughput sequence identification

226 Using next-generation sequencing (NGS), the high-fidelity mutations were demonstrated to b

227 introduction of next-generation sequencing (NGS), these approaches can be revisited to integrate the

228 Next-generation sequencing (NGS), which allows the simultaneous sequencing of billio

229 d microarray and next generation sequencing (NGS)-based approaches to determine whether CBD would alt

230 on, we conducted next-generation sequencing (NGS)-based typing of the 33 human leukocyte antigen (HLA

231 tion followed by next-generation sequencing (NGS).

232 tissue slide via next-generation sequencing (NGS).

233 tide library and next-generation sequencing (NGS).

234 analyzed through next-generation sequencing (NGS).

235 smid ID) through next generation sequencing (NGS).

236 ping or targeted next-generation sequencing (NGS).

237 junctions using next-generation sequencing (NGS).

238 for metagenomic Next Generation Sequencing (NGS).

239 HPV genotypes by next-generation sequencing (NGS).

240 ough advances in next-generation sequencing (NGS).

241 lance using deep next-generation sequencing (NGS).

242 probes) by using next-generation sequencing (NGS).

243 imp tissue using Next Generation Sequencing (NGS).

244 r sequencing and next-generation sequencing (NGS).

245 0(9)/L, enabling next-generation sequencing (NGS); BTK and PLCG2 mutations were detected in 57% and 1

246 Data from next-generation sequencing (NGS, env region) were used to evaluate genetic linkage o

247 Next-generation genetic sequencing (NGS) technologies facilitate the screening of multiple g

248 ctable mutation (next-generation sequencing [NGS] positive), relapse (3-year cumulative incidence, 19

249 dual variant characteristics, and sequential NGS assays to identify potential germline variants.

250 ed amplicon and unbiased metagenomic shotgun NGS approaches.

251 Given a single NGS dataset in BAM format and a pre-compiled VCF-file of

252 SPD-NGS is rapid, reproducible, simple to perform and analyz

253 We applied SPD-NGS to two classes of proteases, the intracellular caspa

254 A second SPD-NGS library (Lib hP), which displayed virtually the enti

255 coupled with next generation sequencing (SPD-NGS) that allows up to 10,000-fold deeper sequence cover

256 Administration of Fzd subtype-specific NGS Wnt in vivo reveals that adult intestinal crypt prol

257 We also use a standard NGS pipeline to show that the mutagenic effect of favipi

258 owever, the limit of resolution for standard NGS is poor.

259 , information traditionally lost in standard NGS library preparation methods.

260 ubtype-specific "next-generation surrogate" (NGS) Wnts that hetero-dimerize Fzd and Lrp6.

261 009 collapse of the Nathorst Glacier System (NGS) in Svalbard, we show that an underlying condition f

262 Targeted NGS on MinION is a promising DST solution for rapidly pr

263 able of running existing TB WGS and targeted NGS library preparations with comparable accuracy to the

264 We performed targeted NGS using the UCSF500 Cancer Panel (University of Califo

265 Next-generation sequencing technology (NGS) enables the discovery of nearly all genetic variant

266 th next generation of sequencing technology (NGS) to analyze protein interactions with DNA.

267 onsequently, there is much anticipation that NGS technologies may be harnessed in the realm of diagno

268 gh consistency of NGS data demonstrates that NGS analysis can be used as high-resolution test alterna

269 The NGS results contrast with previously reported population

270 es of selected resistant enzymes confirm the NGS data and further show that the anti-cancer quinolone

271 iants at very low levels, artifacts from the NGS workflow have to be eliminated.

272 utations were detected in 57% and 13% of the NGS samples, respectively.

273 This Review provides an overview of the NGS technologies available, showcases important advances

274 cantly reduces the space needed to store the NGS results.

275 Surprisingly the NGS results also suggested the presence of the bipartite

276 oncerned, likely due to differences in their NGS read quality control strategies.

277 bilateral retinoblastoma (n = 12), and these NGS results were 100% concordant with commercial germlin

278 However, these NGS assays have yet to be practical methods for patient

279 The scale of these NGS datasets has made it challenging to search through t

280 Thus, NGS Wnts offer a unified organoid expansion protocol and

281 ning clinical-grade biomarkers (blood/tissue NGS, specific immunohistochemistry/RNA expression includ

282 elated phenotype or ocular albinism prior to NGS.

283 ta on a per-individual basis that transforms NGS data interpretation from variant-level to gene-level

284 ne post vaccine strain, RVA/Human-wt/RWA/UFS-NGS:MRC-DPRU442/2012/G1P[8], exhibited a RotaTeq vaccine

285 We reasoned we could use NGS to compare the infection of resting and activated CD

286 following first-line FCR treatment, we used NGS (Adaptive Biotechnologies Corporation) to assess MRD

287 average pairwise diversity calculated using NGS sequences provided a more exact prediction of the ti

288 vel kinase domain mutations identified using NGS in patients with chronic myeloid leukaemia.

289 developed to detect viral integrations using NGS data.

290 essful analysis of CN in FFPE material using NGS provides proof of principle for intensive examinatio

291 these studies highlight the utility of using NGS-based methods to rapidly map drug resistance landsca

292 opular computational frameworks that utilize NGS data as input to decipher microbial composition, whi

293 versus-all sequencing (AVA-Seq) and utilizes NGS to remove multiple bottlenecks of the two-hybrid sys

294 uestions that can be answered when utilizing NGS and bioinformatics.

295 We applied a validated NGS dementia panel to 3241 patients with dementia and he

296 Here, we test the hypothesis whether NGS-generated data could provide a reliable tool to ensu

297 7 accrual sites were analyzed centrally with NGS and selected immunohistochemistry in a master screen

298 horts of unselected populations coupled with NGS data.

299 are significant challenges when dealing with NGS data.

300 In tumor tissue tested with NGS, biallelic inactivation of RB1 was identified in 28