ライフサイエンスコーパス: O-antigen

1 an LPS core oligosaccharide plus one unit of O antigen).

2 needed for the biosynthesis of the core and O-antigen).

3 mposed of lipid A, core oligosaccharide, and O antigen.

4 of E. coli K-12 strain MG1655 to express the O antigen.

5 d by vaccine strains containing a homologous O antigen.

6 not elicit bactericidal antibodies that bind O antigen.

7 -Ss, which stably expresses S. sonnei form I O antigen.

8 the amount, structure or chain length of the O-antigen.

9 , since a gtr(BTP) (1) mutant yields a short O-antigen.

10 lity of sera to kill strains with long-chain O-antigen.

11 illing produce lipopolysaccharide containing O-antigen.

12 profiling to screen a mAb targeting the O25b O-antigen.

13 howing S-layer arrangement at the tip of the O-antigen.

14 in complex with a repeating unit of the O157 O-antigen.

15 f the LPS; thus, LPS from waaL mutants lacks O-antigen.

16 mutant expresses only one repeating unit of O-antigen.

17 s specificity for the Salmonella Typhimurium O-antigen.

18 T16 (pCZ1) mainly expressed the heterologous O-antigen.

19 pCZ1) efficiently expressed the heterologous O-antigen.

20 -linked glycoproteins and lipopolysaccharide O-antigen.

21 for forming the repeat unit domains of these O-antigens.

22 , a truncated core LPS mutant with an intact O antigen, a capsule mutant, and a poly-N-acetylglucosam

23 The O antigen, a component of the surface lipopolysaccharide

24 particular of the O-acetylation state of the O-antigen, a factor that can play an important role in v

25 polymerase mutants) the low molecular weight O-antigen accumulates in the periplasm of the mutant.

26 ults showed that in the absence of opsonins, O antigen allows B. parapertussis to inhibit phagolysoso

27 cular, we compare transporters implicated in O antigen and capsular polysaccharide biosyntheses with

28 body bound predominantly monovalently to the O antigen and reduced bacterial growth without causing c

29 arental or other strains, the biofilm of the O antigen and the PGA mutants was dramatically reduced,

30 to the cell through interactions between the O antigen and two of the six tailspikes; the tail needle

31 type Schu S4 and exhibited marked defects in O-antigen and capsular polysaccharide biosynthesis.

32 A2 or FTT1236/FTL0708-genes required for LPS O-antigen and capsule biosynthesis.

33 surface attachment than mutants deficient in O-antigen and capsule-like complex.

34 d transport also prevented the expression of O-antigen and conferred resistance to HGG1.

35 PF4 bound strongest to mutants lacking the O-antigen and core structure of LPS, but still exposing

36 esized different glycoconjugates, by linking O-antigen and core sugars (OAg) of LPS to the nontoxic m

37 have been elucidated, the effect of removing O-antigen and core sugars on the virulence and immunogen

38 eletion mutant, we revisited the role of LPS O-antigen and demonstrate that it is important for glidi

39 l cell envelope components, such as capsule, O-antigen and lipid A, are often essential for the succe

40 the key enzymes in M. xanthus implicated in O-antigen and lipopolysaccharide (LPS) biosynthesis and

41 The structure of the O-antigen and the core components of the LPS and their p

42 nctional assays suggest that the hydrophilic O-antigen and the core oligosaccharide of the LPS may pa

43 rculosis-CD209 interactions by expression of O-antigen and with oligosaccharides reduces infectivity.

44 linkage between the polysaccharide (core and O-antigen) and lipid A moieties of LPS.

45 nsferase involved in the biosynthesis of the O-antigen) and ManBcoreproteins (phosphomannomutase invo

46 type IV secretion system, a perosamine-based O antigen, and systems for sequestering metal ions that

47 o include trimeric autotransporter adhesins, O antigens, and type IV pili (T4P).

48 s, due to a defect in the lipopolysaccharide O-antigen, and identify a mutation associated with this

49 Thus, molecules other than O antigen are important in triggering dramatic pseudopod

50 transporter-dependent biosynthesis pathway, O antigens are assembled on the cytosolic side of the in

51 O antigens are important cell surface polysaccharides in

52 Fully assembled lipid-linked O antigens are translocated across the inner membrane by

53 As the core and O-antigen are added, per-lipid area increases and lipid

54 igenically variable lipopolysaccharide (LPS) O antigens as a primary effector.

55 ommon antigen (A band) and the heteropolymer O antigen (B band), which impart serospecificity.

56 1 bond resulted in significantly higher anti O-antigen bactericidal antibody titers than coupling to

57 hGBP1 to the bacterial surface disrupts the O-antigen barrier, thereby unmasking lipid A, eliciting

58 assessing responses to vaccines, especially O-antigen-based vaccines, and that the Salmonellagtr rep

59 rter to likely modulate ATPase activity upon O antigen binding.

60 s S. boydii due to nonfunctional S. flexneri O antigen biosynthesis genes.

61 E. coli harboring the R. terrigena O-antigen biosynthesis genes produced an O-antigen displ

62 In this model, O-antigen biosynthesis is initiated with the addition of

63 , which is required for flagellin glycan and O-antigen biosynthesis, was essential for bacterial viab

64 es because of its roles in peptidoglycan and O-antigen biosynthesis.

65 sis a gene from the lipopolysaccharide (LPS) O-antigen biosynthetic cluster which is involved in flag

66 -antigen polymerase, which is located in the O-antigen biosynthetic locus of Francisella tularensis.

67 These included genes encoding the O-antigen biosynthetic pathway, an intact Type 1 Secreti

68 arming by promoting wettability, the loss of O antigen blocks a regulatory pathway that links surface

69 rain HS206 did not bind substantially to A/B/O antigens by synthetic HBGA binding, hemagglutination,

70 0 involves binding to and digesting the O157 O-antigen by TSP2.

71 The composition of the O-antigen can be modified by the activity of glycosyltra

72 several fundamental ways, in terms of their O-antigen, capsulation phenotype, and the genomic island

73 assembly of group 4 polysaccharide capsule (O-antigen capsule).

74 utants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to

75 haride biosynthesis and that the Francisella O antigen, capsule, or both are important for avoiding t

76 ore oligosaccharide branch not linked to the O-antigen causing an increase in overall negative charge

77 e production of preferred lipopolysaccharide O antigen chain lengths is important for the survival of

78 ich encodes the protein that determines long O-antigen chain length and confers resistance to complem

79 lation of 4-aminoarabinose incorporation and O-antigen chain length to respond against the host defen

80 their products are required to determine the O-antigen chain length.

81 ture (OpvAB(OFF)) and a lineage with shorter O-antigen chains (OpvAB(ON)).

82 et7, at permissive temperatures TSPs mediate O-antigen cleavage and couple cell surface binding with

83 endorhamnosidase domain and thus may mediate O-antigen cleavage.

84 Another O-antigen cluster gene, rmlB, which is required for flag

85 utants with disruptions in specific ddhD-wzz O-antigen cluster genes produced LPS that was indistingu

86 by itself and other phage that uses the same O-antigen co-receptor.

87 cle opening is thus a process independent of O-antigen composition and the recognizing TSP.

88 detect and characterize an evolution of the O-antigen composition, in particular of the O-acetylatio

89 The O-antigen concentration of mixed LPS bilayers does not h

90 PS0/10 bilayer simulations show that the LPS O-antigen conformations at a higher concentration of LPS

91 Clinical proof of concept of a 4-valent O antigen conjugate vaccine is ongoing.

92 n technology it is expected that multivalent O antigen conjugate vaccines can be produced at industri

93 s not displaying a polysaccharide capsule or O-antigen-containing lipopolysaccharide, a trait commonl

94 ntly reported the development of a candidate O-antigen-CRM197 glycoconjugate vaccine against S. Typhi

95 In conclusion, an O-antigen-CRM197 glycoconjugate vaccine can induce O-ant

96 d-type B. parapertussis bacteria but not the O antigen-deficient mutants were found colocalizing with

97 Opsonization of O-antigen-deficient LVS in serum lacking terminal comple

98 Unlike parental bacteria, O-antigen-deficient LVS is efficiently killed by serum w

99 ting binding phenotype was linked with lower O-antigen density, enhanced antibody-mediated phagocytos

100 that infection by this strain may not be A/B/O antigen dependent or that in vitro binding patterns do

101 agolysosomal maturation in a lipid raft- and O antigen-dependent manner.

102 y surface plasmon resonance, but the lack of O antigen did not abolish these interactions.

103 ena O-antigen biosynthesis genes produced an O-antigen displaying reduced reactivity toward antisera

104 Despite carrying the O-antigen essential inB. melitensisvirulence, the core d

105 ed in loss of several genetic traits such as O antigen expression.

106 plementation of these rough mutants returned O-antigen expression and susceptibility to amoebae.

107 Our study demonstrates that heterologous O-antigen expression is a promising strategy for the dev

108 A quadrivalent vaccine with O antigens from S. sonnei, S. flexneri 2a, S. flexneri 3

109 71860) is a bioconjugate vaccine, containing O-antigens from Escherichia coli serotypes O1A, O2, O6A,

110 h the recombinant expression of heterologous O-antigens from Salmonella Choleraesuis in Salmonella Ty

111 We recombineered the S. sonnei form I O-antigen gene cluster into the Ty21a chromosome to crea

112 pCZ1 with the cloned Salmonella Choleraesuis O-antigen gene cluster was introduced into three constru

113 Transcription of genes in the ddhD-wzz O-antigen gene cluster, but not core oligosaccharide gen

114 l, attachment invasion locus gene, and rfbC, O-antigen gene, was increased.

115 se systems coexpressed with S. sonnei form I O-antigen gene.

116 Within the known O-antigen genetic clusters of this strain, nine open rea

117 hanistic barriers for bacteriophage-mediated O-antigen glucosylation in ABC transporter-dependent pat

118 modify the structures of lipopolysaccharide O-antigen glycans, altering the structure of the bacteri

119 Including the O-antigen greatly reduces the flexibility of the OprH lo

120 The structure of the O antigen has been determined, but the roles of specific

121 e products involved in synthesis of core and O-antigen have been elucidated, the effect of removing O

122 tial virulence genes for the biosynthesis of O antigens, hemolysins, and exonucleases as well as othe

123 orresponding to three repeating units of the O-antigen in complex with Sf6TSP were studied computatio

124 Salmonella regulates the proportion of long O-antigen in its LPS to respond to the different conditi

125 S) biosynthesis and examined the role of LPS O-antigen in M. xanthus social behaviors.

126 units of ECA at the position occupied by the O-antigen in the case of smooth S. sonnei phase I.

127 a decrease in LPS content and defects in the O-antigen incorporation.

128 Additionally, we establish that long O-antigen inhibits growth in bile only in the presence o

129 The Rhizobium etli CE3 O antigen is a fixed-length heteropolymer.

130 ns of many phenotypes and the density of the O antigen is critical for the observed phenotypes.

131 Thus, preventing the production of O-antigen is a pathway for producing resistance to grazi

132 The E coli O-antigen is a promising vaccine target.

133 enzymatic degradation or modification of the O-antigen is followed by phage infection.

134 For other phages, O-antigen is required for phage infection.

135 LVS DeltacapB retains the immunoprotective O antigen, is serum resistant, and is outgrown by parent

136 haride (LPS), composed of lipid A, core, and O-antigen, is a major virulence factor of Salmonella ent

137 gG, although not required for binding to the O-antigen, is required for motility inhibition.

138 ive strains lack both capsule production and O-antigen laddering.

139 to acquire specific adhesins to overcome the O-antigen layer.

140 GtrC(BTP) (1) is essential for maintaining O-antigen length in isolate D23580, since a gtr(BTP) (1)

141 xamining the role of regulated variations in O-antigen length in the lipopolysaccharide (LPS), a glyc

142 Finally, expression of O-antigen Lewis X and Y epitopes, known to mimic glycoco

143 identified a point-mutation in the gene for O-antigen ligase (WaaL) in Escherichia coli that causes

144 (ABC) sugar transport protein (wzt), and the O-antigen ligase (waaL).

145 lysaccharide (LPS) structure by deleting the O-antigen ligase gene (waaL) enhanced proinflammatory cy

146 In these studies, an O-antigen ligase mutant, waaL, was utilized to determine

147 also identified WaaL(Mx) (MXAN_2919), as the O-antigen ligase that joins O-antigen to lipid A-core.

148 ted a transposon insertion in waaL, encoding O-antigen ligase, that resulted in a loss of swarming bu

149 ve acetyltransferase gene located within the O-antigen lipopolysaccharide cluster.

150 Well-characterized mutants were used: an O-antigen LPS mutant, a truncated core LPS mutant with a

151 virulence genes, including hrpD6, hpaF, the O-antigen LPS synthesis gene rfbC, and the catalase gene

152 ydrate of the P. aeruginosa PAO1 (O5) B-band O-antigen, ManNAc(3NAc)A, has been shown to be critical

153 sting that bactericidal antibodies targeting O antigen may generate universal immunity that could be

154 Therefore, although O antigen may serve a role in swarming by promoting wett

155 The glucosylated form of the O-antigen mediated enhanced survival in human serum and

156 Strikingly, WarA influences P. aeruginosa O antigen modal distribution and interacts with the LPS

157 mine whether structural changes underlie the O-antigen modal length specification, we have determined

158 O-antigen modification was dependent on a functional ABC

159 prophage encodes a gtrC gene, implicated in O-antigen modification.

160 Our results emphasize that natural O-antigen modifications should be taken into considerati

161 In murine immunization studies, both O-antigen modifications were generally immunodominant.

162 ifs and that SpGH95(C) is specific for the H(O)-antigen motif.

163 R, and cpxA mRNA in the biofilm cells of the O-antigen mutant compared to that in the biofilm cells o

164 n capsular mutants and to a lesser degree in O-antigen mutants.

165 and in vivo protection against strains with O antigens not expressed by the vaccine strains, whereas

166 polymeric IgA antibody directed against the O antigen (O-Ag) of Salmonella enterica serovar Typhimur

167 hat directly binds caspases by involving its O-antigen (O Ag) moiety.

168 Such capsules are known as O-antigen (O-Ag) capsules, due to their high degree of s

169 t binds an immunodominant epitope within the O-antigen (O-Ag) component of lipopolysaccharide.

170 Lipopolysaccharide (LPS) O-antigen (O-Ag) is known to limit antibody binding to s

171 glycosyltransferase with a single subunit of O-antigen (O-ag).

172 ularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex f

173 The lipopolysaccharide (LPS) O antigen (OAg) of F. tularensis has been considered for

174 ternal repeat units of the O-polysaccharide [O-antigen (OAg)] of Ft LPS.

175 ation patterns associated with Bp and Bm LPS O-antigens (OAgs).

176 results indicate that binding of IgG to the O antigen of C. turicensis causes a direct antimicrobial

177 nd wbtI genes essential for synthesis of the O antigen of lipopolysaccharide.

178 rious secondary metabolites as well as K and O antigens of bacterial lipopolysaccharide.

179 hemical synthesis of the complete protective O-antigen of a human-disease-causing pathogenic bacteriu

180 monoclonal antibody (mAb) targeting the O25b O-antigen of Escherichia coli ST131.

181 Caulobacter crescentus S-layer bound to the O-antigen of lipopolysaccharide.

182 The short O-antigen of the gtr(BTP) (1) mutant was also compensate

183 dy nanovalve with a tetrasaccharide from the O-antigen of the lipopolysaccharide (LPS) of Francisella

184 ccharide component of the lipopolysaccharide O-antigen of the nitrogen-fixing bacteria Bradyrhizobium

185 bits motility by specifically binding to the O-antigen of V. cholerae We demonstrate that the bivalen

186 e repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27

187 ity of a bioconjugate vaccine containing the O-antigens of four E coli serotypes (ExPEC4V).

188 O-antigens of Gram-negative bacteria modulate the intera

189 mine is an unusual dideoxysugar found in the O-antigens of some Gram-negative bacteria, including the

190 Full chemical analysis of the O-antigens of these strains identified gtr-dependent glu

191 ics simulations, we deduce the length of the O-antigen on cells and show how lipopolysaccharide bindi

192 ical study, we follow the composition of the O-antigen on the outer bacterial membrane with high-reso

193 The WaaL-mediated ligation of O-antigen onto the core region of the lipid A-core block

194 Wild-type and O-antigen or capsular mutants of K. pneumoniae were grow

195 iofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly

196 sence of insertion sequences or mutations in O-antigen or lipopolysaccharide core biosynthesis genes,

197 gene, which controls production of very long O-antigen, or other PmrA-activated genes that mediate mo

198 mination with extracellular polysaccharides, O-antigen, or storage compounds.

199 Thus, the O antigen plays a role in the morphology of uptake in th

200 t demonstrated that (as is characteristic of O-antigen polymerase mutants) the low molecular weight O

201 The O-antigen polymerase of gram-negative bacteria has been

202 ssessment) strongly suggested that Wzy is an O-antigen polymerase whose function is to catalyze the a

203 A wzy (O-antigen polymerase) deletion mutant of Ft. live attenu

204 (Wzy) of the gene annotated as the putative O-antigen polymerase, which is located in the O-antigen

205 The surface O-antigen polymers of gram-negative bacteria exhibit a m

206 enuation in its ability to produce very long O-antigen polymers.

207 In Gram-negative bacteria, the O antigen polysaccharide represents the variable region

208 The O-antigen polysaccharide (O-PS) component of lipopolysac

209 The Escherichia coli serotype O9a O-antigen polysaccharide (O-PS) is a model for glycan bi

210 In contrast, the long-chain O-antigen polysaccharide (O-PS) shows remarkable structu

211 of lipid A, core oligosaccharide (C-OS), and O-antigen polysaccharide (O-PS).

212 non-repeating core oligosaccharide, and the O-antigen polysaccharide is the most exposed component o

213 n and is modified with sugars mediated by an O-antigen polysaccharide ligase (WaaL) that is associate

214 and hydrolyzes the high-rhamnose, serotype Y O-antigen polysaccharide of the Gram-negative bacterium

215 cross-relaxation rates are obtained from an O-antigen polysaccharide sample.

216 is revealed a 19-gene locus, involved in LPS O-antigen polysaccharide synthesis and conserved among m

217 pid A insertion and core oligosaccharide and O-antigen polysaccharide translocation, respectively.

218 regions: lipid A, core oligosaccharide, and O-antigen polysaccharide.

219 inner core oligosaccharide, and a repeating O-antigen polysaccharide.

220 Raoultella terrigena ATCC 33257 produces an O-antigen possessing the same disaccharide motif, but it

221 study the glucosylation potential of a model O-antigen produced in an ATP-binding cassette (ABC) tran

222 Importantly, O antigen production enables laboratory strains of E. co

223 We demonstrate that O antigen production results in drastic alterations of m

224 e demonstrate that the temperature-sensitive O-antigen production defect in 2192 is due to a mutation

225 time that a temperature-sensitive defect in O-antigen production has been reported.

226 Although O-antigen production is cryptic in Escherichia coli K-12

227 d strains that are disrupted for capsule and O-antigen production.

228 (1) mediates modification of the BTP1 phage O-antigen receptor in lysogenic D23580, and thereby prev

229 t7 was triggered by lipopolysaccharide (LPS) O-antigen receptors and notably slower than in noncontra

230 e structure provides important insights into O antigen recruitment to and translocation by WzmWzt and

231 In conclusion, the absence of the O antigen reduces the ability of A. pleuropneumoniae to

232 e exposed LPS core through the expression of O-antigen reduces dissemination and infection.

233 is important for the formation of the short O-antigen region.

234 a mixture of long and very long polymers of O antigen, regulated by two different genes.

235 al Escherichia coli lipopolysaccharide (LPS) O antigen regulator WbdD.

236 showed that the V. fischeri LPS has a single O-antigen repeat composed of yersiniose, 8-epi-legionami

237 eGlcA) to complete the first instance of the O-antigen repeat unit.

238 The O-antigen repeating unit (O-unit) of Escherichia coli O8

239 nas aeruginosa 1244 pilin by adding a single O-antigen repeating unit to the beta carbon of the C-ter

240 o catalyze the addition of newly synthesized O-antigen repeating units to a glycolipid consisting of

241 N) with its ligands comprising two and three O-antigen repeats from Salmonella enterica serovar Typhi

242 gest the O157, O77, and O78 Escherichia coli O-antigens, respectively.

243 Depletion of IgG2 to O-antigen restores the ability of sera to kill strains w

244 depends on the chemical modification of the O antigen's nonreducing terminus, sensed by WzmWzt via a

245 sources to implement a combination PCR-based O-antigen screening and sequencing of internal fliC and

246 he outer membrane of gram-negative bacteria, O-antigen segments of lipopolysaccharide (LPS) form a ch

247 The Escherichia coli O9a and O8 O-antigen serotypes represent model systems for the ABC

248 nclude the involvement of specific clones or O-antigen serotypes, the presence of certain horizontall

249 valently linked to Shigella flexneri type 2a O-antigen (Sf2E) produced by engineered Escherichia coli

250 We suggest that excessive binding of IgG2 to O-antigen shields the bacterium from other antibodies th

251 omer can lead to shifts in the length of the O antigen side chain.

252 The O-antigen side chain of the lipopolysaccharide is an imm

253 were assessed using lipopolysaccharide (LPS) O antigen-specific antibodies.

254 rface proteins augmented the contribution of O-antigen-specific antibodies elicited by vaccine strain

255 gen-CRM197 glycoconjugate vaccine can induce O-antigen-specific antibodies of different isotypes that

256 munologic interference in the development of O-antigen-specific antibody responses when closely relat

257 ed killing is caused by excess production of O-antigen-specific IgG2 antibodies.

258 t LPS sugar compositions and reactivity with O-antigen-specific monoclonal antibodies.

259 eriophage with a contractile tail, the multi-O-antigen-specific Salmonella myovirus Det7.

260 as already verified here for Shigella sonnei O-antigen, Streptococcus pneumoniae serotype 12F, and St

261 As there is significant sharing of O-antigen structure between different Gram-negative bact

262 O antigen structures are serotype specific and form exte

263 tudies indicate that the unusual V. fischeri O-antigen sugars play a role in the early phases of bact

264 ule structures, and not a lipopolysaccharide O antigen, supported by the fact that they contain genes

265 n pathway and its possible relationship with O-antigen surface sensing, mutations were made in the rc

266 Mutations in three other genes involved in O-antigen synthesis and transport also prevented the exp

267 esis operon (pso) abolish lipopolysaccharide O-antigen synthesis and Weil-Felix serology and alter ou

268 mutations that block different steps in LPS O-antigen synthesis can cause pleiotropic phenotypes.

269 the AcrAB efflux pump and lipopolysaccharide O-antigen synthesis for bile salt resistance.

270 WbbB, a modular protein participating in LPS O-antigen synthesis in Raoultella terrigena The beta-Kdo

271 s/deletions or point mutations identified in O-antigen synthesis or modification genes, rendering the

272 genotypically serotyped using PCR targeting O-antigen synthesis or modification genes.

273 dent pathways, one of the two most prevalent O-antigen synthesis systems.

274 with an unusual defect in lipopolysaccharide O-antigen synthesis, but loss of FTL_0325 was not.

275 tant lacking the endogenous WbaP that primes O-antigen synthesis, indicating that WbaP(Mx) transfers

276 sed on these results and current theories of O-antigen synthesis, specific roles were deduced for eac

277 des a UDP-4,6-GlcNAc dehydratase involved in O-antigen synthesis.

278 hosphate transferase responsible for priming O-antigen synthesis.

279 ate, glucosylation has only been observed in O-antigens synthesized by Wzy-dependent pathways, one of

280 The O antigen targets B. parapertussis to lipid rafts that a

281 DS-PAGE revealed stepwise truncations of the O antigen that were consistent with differences in mutan

282 We identify human monoclonal antibodies to O-antigens that are highly protective in mouse models of

283 core sugar biosynthesis and the ligation of O antigen to the LPS core sugars.

284 he impact of coupling Salmonella typhimurium O-antigen to different amino acids of CRM197 protein car

285 AN_2919), as the O-antigen ligase that joins O-antigen to lipid A-core.

286 WaaL ligates the O-antigen to the core of the LPS; thus, LPS from waaL mu

287 zer-resistant knockout mutant of the wzm ABC O-antigen transporter gene, SynPCC7942_1126.

288 roduce an oligosaccharide core with a single O-antigen unit attached in an RfaH-dependent fashion.

289 vaccine, while a wzy mutant that retains one O-antigen unit is adequate for stimulating the optimal p

290 4P) (5) , two of which are glycosylated with O-antigen units (6) or polymers of D-arabinofuranose (7-

291 d that Det7 recognized the Salmonella Anatum O-antigen via an e15-like TSP, DettilonTSP.

292 aaP, waaY, and rfaQ), outer core (rfaG), and O-antigen (waaL, wzzE, and wzyE).

293 a model for biosynthesis of the R. etli CE3 O antigen was proposed.

294 Production of a fully extended O-antigen was also diminished in a Kdo hydrolase mutant,

295 ific antibody responses when closely related O antigens were combined in multivalent vaccines.

296 In contrast, 2192 expresses no LPS O antigen when grown at 37 degrees C.

297 pressed both the homologous and heterologous O-antigens, whereas in the absence of arabinose, SLT16 (

298 tisic acid; while A galU mutant is devoid of O-antigen, which is required for phage adsorption.

299 nduced specific IgG against the heterologous O-antigen, which mediated significant killing of Salmone

300 Finally, the O antigen would be capped by attachment of di- or tri-O-