ライフサイエンスコーパス: PCR

1 PCR rejection criteria, based on white blood cell (WBC)

2 PCR was used to confirm viral replication in SOT-recipie

3 PCR-EEM analysis trained on the IGFR samples was applied

4 PCRs were positive in 27/5,594 (0.5%) bacterial culture-

5 of PCR primer pairs (producing up to 23,040 PCR products) during a single thermal cycling protocol.

6 negative samples, mean cost-per-positive 16S PCR result was pound 568.37 and pound 292.84 for targete

7 to compare Veritor with the Lyra SARS-CoV-2 PCR assay (Lyra).

8 SARS-CoV-2 PCR positive samples were cultured in Vero C1008 cells a

9 c individuals to predict positive SARS-CoV-2 PCR, and likelihood ratios for each CO-RADS score were u

10 plus 64 pandemic-era samples from SARS-CoV-2 PCR-negative patients with respiratory symptoms.

11 at least 14 days after a positive SARS-CoV-2 PCR.

12 A PCR-based diagnostic test was developed and confirmed fu

13 equations to estimate the median ACR from a PCR, optionally including specified covariates.

14 We first purify the bacterial DNA into a PCR solution using our DM-based sample preparation.

15 contaminated chicken meat, without needing a PCR step.

16 subset of 87 cases that underwent additional PCR- and immunohistochemical testing to define a sensiti

17 t, consisting of paired sera collected after PCR-confirmation of infection (n = 94).

18 Adjusted VE against PCR-diagnosed pertussis was 52% (95% CI, 15-73%), nonsig

19 of bias are the synthesis and amplification (PCR) processes.

20 ad simultaneous assessments of urine ACR and PCR.

21 spected sepsis (sepsis-2 definition), BC and PCR/ESI-MS on whole blood were positive in 14.6% and 25.

22 CGG repeat allele size by Southern Blot and PCR analysis.

23 e correlation between SARS-CoV-2 culture and PCR.

24 cence, fluorescent in situ hybridization and PCR-based investigations.

25 ive llama single-domain antibody library and PCR-based maturation, we have produced two closely relat

26 /ESI-MS positive, 15.3%; and BC negative and PCR/ESI-MS negative, 70.1%.

27 PCR/ESI-MS positive, 10.3%; BC negative and PCR/ESI-MS positive, 15.3%; and BC negative and PCR/ESI-

28 llowing result combinations: BC positive and PCR/ESI-MS negative, 4.3%; BC positive and PCR/ESI-MS po

29 d PCR/ESI-MS negative, 4.3%; BC positive and PCR/ESI-MS positive, 10.3%; BC negative and PCR/ESI-MS p

30 Genome skimming and PCR allowed us to retrieve the plastome, 57 single copy

31 AP, fluorescence-activated cell sorting, and PCR.

32 roposed, generally relying on multi-step and PCR-based, time-consuming and cost-ineffective procedure

33 een extremely slow, and key reagents such as PCR primers and RNA extraction kits are at critical shor

34 B/RIF Ultra (Xpert Ultra), a fully automated PCR assay, as the initial tuberculous meningitis (TBM) d

35 lassic bacteriology, immunoassays, gel-based PCR and reverse transcriptase PCR, and quantitative real

36 The SSRs are robust (with basic PCR methods and agarose gel electrophoresis), informativ

37 Compared with BC, PCR/ESI-MS showed the following sensitivities (coagulase

38 First, we demonstrated through both PCR and immunostaining that mouse lymphatic muscle cells

39 ulture and Elek, 6 were culture-negative but PCR-positive for diphtheria toxin gene, 1 was culture-po

40 8 human-source E. coli strains classified by PCR as ExPEC and non-ExPEC.

41 The true status of pigs was established by PCR testing on oral fluids samples over the course of th

42 Compared to bacterial culture followed by PCR identification of resistance genes from colonies, th

43 ransmission, not the fragments identified by PCR.

44 alyzed by enzyme-immunoassays; viral load by PCR.

45 were expressed in mouse lymphatic muscle by PCR, but only Kir6.1 was expressed in lymphatic endothel

46 days after a positive nasopharyngeal test by PCR with reverse transcription.

47 Finally, chromatin-capture PCR confirmed long-range co-regulation of SOCS2 and FLT3

48 es of noise owing to incomplete RNA capture, PCR amplification biases and/or batch effects specific t

49 Transcriptomic analysis and ChIP-PCR together demonstrated that protein kinase D1 (Prkd1)

50 In a case series of 12 patients with culture/PCR-negative body fluids but for whom an infectious diag

51 The 42-day PCR corrected efficacy of dihydroartemisinin-piperaquine

52 e aim of this study was to develop a digital PCR (ddPCR) assay discriminating between circular and in

53 2 RNAaemia measured by droplet-based digital PCR constitutes a promising prognosis biomarker in COVID

54 oes not reduce NA yield (measured by digital PCR).

55 Agreement between ctDNA digital PCR and targeted sequencing was 96-99% (n=800, kappa 0.8

56 e tested novel linkage-based droplet digital PCR (ddPCR) assays to study 20 inversions ranging from 3

57 g, mutation-specific PCR, or droplet digital PCR to determine the presence of BRAF(V600), NRAS(Q61),

58 ed controls using MethyLight droplet digital PCR.

59 Conversely the accuracy of digital PCR is diminished at higher concentrations of template a

60 l taxa by combining the precision of digital PCR with the high-throughput nature of 16S rRNA gene amp

61 igh-resolution melt (dHRM) after the digital PCR (dPCR).

62 pant results were analyzed by bi-directional PCR/sequencing of residual blood culture samples.

63 Individual digital droplet PCR assays were developed for 121 variants (average 5/pa

64 p sequencing of 25 genes and Digital Droplet PCR of TERT promoter, including sequential samples throu

65 We compared six "next-gen" VOA employing PCR or ultrasensitive p24 to assess their suitability as

66 d in a myriad of emulsion droplets (emulsion PCR, ePCR) allows the mitigation of this problem.

67 Following PCR amplification with indexing primers, the subnucleoso

68 e is presented as an alternative readout for PCR utilizing NAzymes.

69 as estimated ACR that had been derived from PCR.

70 of pre- and post-influenza season sera from PCR-confirmed influenza cases (n = 50), and (ii) an immu

71 tients without any amplification step (e.g., PCR) required, thus simplifying the operation further.

72 y, using three replicated assignments (e.g., PCRs for microsatellites) per genetic sample.

73 cile versus 49 patients (27%) in the post-GI PCR cohort (P < .001).

74 Eight patients (5%) in the pre-GI PCR cohort tested positive for a pathogen other than C.

75 The high PCR Ct value for all samples (>30) indicated that the vi

76 dications (n = 603) had significantly higher PCR/ESI-MS positivity rates than patients without prior

77 tests could exclude anaplasmosis, improving PCR utilization.

78 hree research teams and their application in PCR-based diagnostics, high-affinity DNA aptamer generat

79 an unmet need due to artifacts generated in PCR-based RNA-Seq library preparation and the lack of no

80 sed-tube Linear-After-The-Exponential (LATE)-PCR assay for pyrazinamide susceptibility in Mycobacteri

81 assay, and the results were compared to mecA PCR results.

82 Using linear amplification-mediated PCR, we successfully map the UBC-GFP transgene to the MH

83 endocarditis, were diagnosed by microscopy, PCR-based detections and culture: we showed M. smithii m

84 Sputum multiplex PCR could become a useful diagnostic tool for bacterial

85 onventional stool testing with a multiplexed PCR assay, without an increase in testing costs.

86 Where no CPE was observed, PCR was performed on day four to confirm absence of viru

87 of the primer set, confirming the absence of PCR-based biases.

88 eral reagents and steps and the high cost of PCR hinder its worldwide implementation to contain the o

89 d regions for 48 DNA samples and hundreds of PCR primer pairs (producing up to 23,040 PCR products) d

90 The cumulative incidence of PCR-adjusted ACPR at Day 42 was 96.1% (95% confidence in

91 hreshold (Ct) values represent the number of PCR cycles required for probe signal to be detected (low

92 isk of COVID-19 and enable prioritization of PCR testing and containment efforts.

93 wever, the conventional Sanger sequencing of PCR products for the authentication of seafood species i

94 Using a high-quality set of PCR-based genotyping of >200 loci, we show that TypeTE i

95 erience on the utility and sustainability of PCR based surveillance testing in areas with receding an

96 Co-transfection of PCR cassettes with a Cas12a-encoding plasmid leads to ro

97 e standard, the negative predictive value of PCR was found to be 100%, while the positive predictive

98 ver, and kidney samples that were ZsG and/or PCR positive, and only from animals euthanized on or bef

99 11/22) of patients recruited were culture or PCR positive for S. Typhi.

100 ePCR outperforms PCR, reduces gross DNA errors, and provides a more unifo

101 ent of a 10-23 DNAzyme into the primer pair, PCR-amplified DNAzyme-amps were generated, tested, and v

102 A amplification and sequencing (panbacterial PCR) for detection and identification of all bacterial s

103 th sampling times 0 to 56 days post-positive PCR (median/mean, 2/6.2 days).

104 valence among 195 participants with positive PCR more than 14 days before the study visit ranged from

105 are test and immunoassay reported a previous PCR test.

106 e, sex, race, county of residence, and prior PCR-confirmed viral exposure.

107 Error-prone PCR mutagenesis reinforced the importance of R225 substi

108 qRT-PCR analysis indicated the expression of G-protein-coupl

109 RNA-Seq and qRT-PCR analyses reveal that auxin-responsive genes and grow

110 expression results using NanoString and qRT-PCR for 18 genes in the same pool of RNA (RNAlater), we

111 flow cytometry, immunocytochemistry, and qRT-PCR showed high neuralization efficiency.

112 a pre-amplification of human RNA before qRT-PCR.

113 the 3'-UTR of mouse MR were profiled by qRT-PCR after aldosterone stimulation.

114 Relevant miRNAs were validated by qRT-PCR and in situ hybridization (ISH).

115 horesis-based size-selection followed by qRT-PCR validated the top six up-regulated tRFs in a separat

116 tested negative for EBOV RNA in blood by qRT-PCR.

117 low 1 female and 1 male gametocyte/uL by qRT-PCR.

118 y (average RIN 6.7 + /- 0.8) allowed for qRT-PCR.

119 Gametocyte sex ratios from qRT-PCR were compared with those from immunofluorescence ass

120 quantitative reverse transcription-PCR (qRT-PCR) subsequently confirmed QS upregulation within 1 h o

121 quantitative reverse-transcriptase PCR (qRT-PCR), and RNA-Seq for PD-1 expression.

122 A-seq data; (v) validation studies using qRT-PCR were conducted on 26 selected representative genes;

123 Quantitative PCR, 16S rRNA gene metabarcoding and shotgun metagenomic

124 ection as there are no accepted quantitative PCR cutoffs for diagnosing respiratory viral infections.

125 Rapid diagnostic test and quantitative PCR (qPCR) were used for the detection of infections tha

126 hybridization for avt-mRNA, and quantitative PCR.

127 f the Pan-LASV RDT to available quantitative PCR (qPCR) assays during the 2018 LF outbreak in Nigeria

128 nt matrix genes was measured by quantitative PCR and also analyzed in single-cell RNA-sequencing data

129 positive for SARS-CoV-2 RNA by quantitative PCR, suggesting community transmission of SARS-CoV2 in W

130 (RNAP) activity using a common quantitative PCR instrument for fluorescence detection.

131 on-sensitive restriction enzyme-quantitative PCR (MSRE-qPCR) and observed that multiple genes of the

132 oreover, tandem affinity and RT-quantitative PCR results revealed that JUND mRNA is a component of a

133 s were tested by multiplex semi-quantitative PCR for 12 viruses.

134 to select bacteria for targeted quantitative PCR (qPCR).

135 designed a multiplex real-time quantitative PCR (qPCR) assay to detect SFGR, TGR, O. tsutsugamushi,

136 was also detected by real-time quantitative PCR comparing lesional with perilesional or healthy skin

137 Real-time quantitative PCR may be useful in differentiating asymptomatic sheddi

138 m 65 individuals with real-time quantitative PCR-confirmed SARS-CoV-2 infection, we show seroconversi

139 HHV-6 viremia using a real-time quantitative PCR.

140 rescence, reverse transcriptase quantitative PCR (RT-qPCR), and NGS.

141 esults to reverse transcriptase quantitative PCR and measurement of fluorescein concentrations (doped

142 amined by reverse-transcriptase quantitative PCR, intracellular flow cytometry, and ELISA.

143 nes using reverse transcription quantitative PCR.

144 trols for enteropathogens using quantitative PCR and calculated pathogen-specific attributable fracti

145 VL was determined using quantitative PCR and log10 transformed for normalization.

146 LOCK was in 100% agreement with quantitative PCR with reverse transcription.

147 libraries for OSC fragments to use in a RACE PCR-based approach and cloned three full-length OSC tran

148 address this challenge, combining long-range PCR and nanopore sequencing with a novel bioinformatics

149 h microscopy- and polymerase chain reaction (PCR) -based methods, was performed monthly, and informat

150 aluation of miRNA polymerase chain reaction (PCR) arrays indicated that the expression of miR-522-3p

151 Multiplexed polymerase chain reaction (PCR) assays increase the detection of diarrheal pathogen

152 While polymerase chain reaction (PCR) detection corresponded well to ZsG signal, virus wa

153 ausative virus by polymerase chain reaction (PCR) even after clinical recovery, thereby complicating

154 The polymerase chain reaction (PCR) has been the gold standard molecular analysis techn

155 tion confirmed by polymerase chain reaction (PCR) in seropositive and seronegative health care worker

156 es using a nested polymerase chain reaction (PCR) protocol that targets the parasite mitochondrial cy

157 ction detected on polymerase chain reaction (PCR) screening of a large homeless shelter population in

158 terizes trends in polymerase chain reaction (PCR) test positivity for severe acute respiratory syndro

159 lts of SARS-CoV-2 polymerase chain reaction (PCR) testing of environmental surfaces and personal prot

160 4) for SARS-CoV-2 polymerase chain reaction (PCR) testing.

161 ion inhibition of polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), and

162 ts diagnosed with polymerase chain reaction (PCR)-confirmed SARS-CoV-2 infection at 4 hospitals in Si

163 NP using realtime polymerase chain reaction (PCR).

164 rticipants from fourteen studies, PQ reduced PCR-determined gametocyte carriage on days 7 and 14, mos

165 16S rRNA PCR/sequencing has higher sensitivity to detect bacteria

166 n compared to species-specific SARS-CoV-2 RT-PCR assays.

167 performance of high-throughput SARS-CoV-2 RT-PCR systems.

168 lternative viral infections in SARS-CoV-2 RT-PCR-negative PUIs (n = 30) and viral coinfections in SAR

169 30) and viral coinfections in SARS-CoV-2 RT-PCR-positive PUIs (n = 45).

170 From March 15 to June 1, 2020, 250 RT-PCR confirmed COVID-19 patients were studied with low-do

171 ausative virus was later sequenced from a RT-PCR-positive individual and assessed using phylogenetic

172 Among RT-PCR-confirmed infections in children, hemagglutination i

173 A total of 104 individuals had an RT-PCR-positive viral test with a cycle threshold (C(T) ) o

174 preoperative screening using chest CT and RT-PCR before elective or emergency surgery under general a

175 tested for SARS-CoV-2 by BinaxNOW TM and RT-PCR in a community setting, rapid assay sensitivity was

176 ly associated with rat IgG positivity and RT-PCR positivity (P = .03 and P = .006, respectively).

177 and RNA viruses using a real-time PCR and RT-PCR.

178 using immunostaining, RNA sequencing, and RT-PCR.

179 expression was analyzed using RNA-Seq and RT-PCR.

180 ased molecular biology techniques such as rt-PCR.

181 GAX) renal lesions that were validated by RT-PCR and IHC.

182 131 (70%) were positive for SARS-CoV-2 by RT-PCR or antibody testing, and 164 (88%) were hospitalized

183 roliferation for subsequent validation by RT-PCR.

184 -75) years, with detectable SARS-CoV-2 by RT-PCR.

185 lected identified genes were confirmed by RT-PCR.

186 This new Corynebacterium RT-PCR method provides a rapid tool to screen isolates and

187 ients for COVID-19 using either chest CT, RT-PCR or both, due to the risk for worsened surgical outco

188 may fill many of the gaps in the current RT-PCR testing regime, especially in low- and middle-income

189 and I38T mutant were assessed by digital RT-PCR.

190 r chest CT and 1.1% (95% CI: 0.6-1.7) for RT-PCR; the incremental yield of chest CT was 0.4%.

191 oded hospitalizations, 1064 (6%) included RT-PCR testing for influenza viruses, 614 (58%) of which we

192 Care must be taken in interpreting RT-PCR tests for SARS-CoV-2 infection-particularly early in

193 P = 0.050) were higher and nasopharyngeal RT-PCR cycle threshold values lower (P = 0.010) in patients

194 d compare tests such as the CDC 2019-nCoV RT-PCR Diagnostic Panel, Cellex's qSARS-CoV-2 IgG/IgM Rapid

195 compare the baseline strategy of (S0) no RT-PCR testing of workers to testing workers (S1) with COVI

196 n should not be ruled out on the basis of RT-PCR alone, and the clinical and epidemiologic situation

197 ults and there is currently a shortage of RT-PCR test kits, underscoring the urgent need for alternat

198 g the current pandemic, the deployment of RT-PCR tests has been extremely slow, and key reagents such

199 However, the proportion of RT-PCR-confirmed COVID-19 cases in the categories atypical

200 nt both initial chest CT and at least one RT-PCR test within 48 hours were included.

201 Reverse transcription PCR (RT-PCR) and sequencing revealed that all US isolates and a

202 age, real-time reverse transcription-PCR (RT-PCR) assays remain the molecular test of choice for the

203 and SARS-CoV-2 reverse transcriptase PCR (RT-PCR) results were compared.

204 Routine EV/PeV RT-PCR testing of cerebrospinal fluid (CSF) samples in chil

205 symptomatic at the date of first positive RT-PCR collection; two (4%) had preceding symptoms that res

206 ncluded, of whom 536 (50%) had a positive RT-PCR result and 137 (13%) of whom were considered to have

207 SARS-CoV-2 RNA, and 50.0% had a positive RT-PCR result.

208 ted symptoms on the day of first positive RT-PCR test were upper respiratory (n=32, 68%) and neurolog

209 In our population with previous RT-PCR confirmed infection, approximately one in 16 persons

210 results from a CDC-approved quantitative RT-PCR (RT-qPCR) assay performed in a state testing lab, an

211 Using Quantitative RT-PCR and Cyquant assay, we showed that miR-124 protects a

212 employing FACS coupled with quantitative RT-PCR, a validated GLP-1R antibody, and flow cytometry to

213 d by microarray analysis and quantitative RT-PCR, and expression of proteins was measured by ELISA an

214 ep RNA-Seq complemented with quantitative RT-PCR, we found that feeding causes substantial and transi

215 NA and mRNA expression using quantitative RT-PCR.

216 sion of ACE2 was measured by quantitative RT-PCR.

217 transcriptase polymerase chain reaction (RT-PCR) are being used to rule out infection among high-ris

218 transcription polymerase chain reaction (RT-PCR) are the gold standard, during the current pandemic,

219 -transcriptase-polymerase-chain-reaction (RT-PCR) assay.

220 transcriptase polymerase chain reaction (RT-PCR) examination of abdominal fluid was negative for the

221 -transcription polymerase chain reaction (RT-PCR) in Australia, Canada, Israel, and the United States

222 transcription polymerase chain reaction (RT-PCR), which can have good sensitivity and excellent spec

223 transcriptase polymerase chain reaction (RT-PCR).

224 transcription polymerase chain reaction (RT-PCR).

225 We hypothesized that modern HCV-RNA RT-PCR platforms would adequately detect infected infants.

226 rat IgG prevalence ranged 2%-70% and SEOV RT-PCR positivity ranged 0%-70%.

227 nfirmed dengue (VCD) by serotype-specific RT-PCR.

228 terature were then quantified by targeted rt-PCR in the complete TRILOGY-ACS cohort (N = 878) and com

229 The RT-PCR did not detect viral RNA in the wall of small intest

230 nostic hypothesis, strongly linked to the RT-PCR results for the "typical," "atypical," and "negative

231 equencing and stem cell pathway real-time RT-PCR analysis revealed profound reductions in WNT1 expres

232 L, making it an attractive alternative to RT-PCR assays.

233 the tails of sheep through transcriptome, RT-PCR, qPCR, and Western blot analyses.

234 ssay sensitivity was 100%/98.5%/89% using RT-PCR Ct thresholds of 30, 35 and none.

235 teen viruses, including SARS-CoV-2, using RT-PCR.

236 Among 1,926 patients, 345 (17.9%) were RT-PCR positive for EV and 172 (8.9%) were positive for PeV

237 f 0.87 (95% CI: 0.84, 0.89) compared with RT-PCR and 0.87 (95% CI: 0.85, 0.89) compared with the clin

238 With RT-PCR test results as the reference standard, the AI syste

239 When compared with RT-PCR, low-dose submillisievert chest CT demonstrated exce

240 agnosis, either laboratory confirmed with RT-PCR, suspected with symptoms and contacts, or radiologic

241 ptoms tested positive for SARS-CoV-2 with RT-PCR; this yield increased in conjunction with community

242 enius Mycoplasma MGB research use only (RUO) PCR, and the SpeeDx Resistance Plus MP assays.

243 The assay's PCR cycle threshold (Ct) values represent the number of

244 ic beads is replaced by a carefully selected PCR solution, enabling direct transfer from sample prepa

245 mol for cow and goat, and 3.1 fmol for sheep PCR product were detected.

246 rimer pairs to identify mold within a single PCR run.

247 M. smithii microscopically and by a specific PCR followed by sequencing method in two of three cardio

248 A multiplex allele-specific PCR targeting outbreak-specific single nucleotide polymo

249 galactomannan testing, Aspergillus-specific PCR, and microscopy and culture.

250 assays, Sanger sequencing, mutation-specific PCR, or droplet digital PCR to determine the presence of

251 ing coupled with an outbreak-strain-specific PCR enabled us to markedly redefine the initial picture

252 irus or pathogenic disease by gold standard (PCR, qPCR, RT-qPCR) methods, therefore, alternative meth

253 ity using 128 serum samples from symptomatic PCR-confirmed coronavirus disease 2019 (COVID-19)-infect

254 s pound 568.37 and pound 292.84 for targeted PCR, equating to pound 4041.76 and pound 1506.03 respect

255 No animals tested PCR positive.

256 The probability that PCR detects DNA of haemosporidian parasite was higher fo

257 Results obtained from the PCR method were successfully validated by chromatographi

258 on of isolation/quarantine, (ii) whether the PCR cycle threshold value should be included on patient

259 We used real time PCR in order to find the relative quantity of glutamate

260 itu hybridization and quantitative real time PCR.

261 sing a previously published duplex real-time PCR (capable of detecting E. histolytica and E. dispar),

262 Quantitative real-time PCR (qPCR) and Western blot analyses confirmed that the

263 fication of all bacterial species; real-time PCR (qPCR) assays targeting the femA or lytA gene for de

264 ranscriptase PCR, and quantitative real-time PCR (qPCR).

265 RNA sequencing and quantitative real-time PCR analysis were used to assess the transcriptional res

266 ed for DNA and RNA viruses using a real-time PCR and RT-PCR.

267 screening, along with quantitative real-time PCR and time-resolved amplicon Illumina MiSeq sequencing

268 We used a species-specific real-time PCR approach to detect C. limbatus eDNA in the bay on a

269 tica and E. dispar), our tetraplex real-time PCR assay demonstrated levels of sensitivity and specifi

270 h those demonstrated by the duplex real-time PCR assay.

271 ensitive in vitro diagnostic (IVD) real-time PCR assays for respiratory viruses, including an assay f

272 Gold standard is the real-time PCR assays, which can be conducted at highly equipped la

273 ecies-specific eDNA analysis using real-time PCR could therefore represent a cost-effective, scalable

274 mplification tests (NAATs) such as real-time PCR demonstrate excellent an limit of detection (LOD) wh

275 Quantitative real-time PCR detected an increase in the levels of immune stimula

276 ers region, was used to design the real-time PCR detection assay, resulting in an 490 bp amplicon wit

277 ole blood samples were analysed by real-time PCR for Borrelia burgdorferi s.l., Borrelia miyamotoi, A

278 rs were determined by quantitative real-time PCR for chymase and c-kit.

279 ther evaluation using quantitative real-time PCR revealed that differentially expressed patterns are

280 Four commercial real-time PCR tests, toxin antigen detection by enzyme immunoassay

281 Real-time PCR was used as a screening tool with great accuracy, wh

282 Real-time PCR was used for quantitative analysis of Aggregatibacte

283 resence of tick-borne pathogens by real-time PCR, and a subset of samples was tested for Borrelia bur

284 n and activity were examined using real-time PCR, immunoblotting and flow cytometry.

285 -cancerous tissues by quantitative Real-Time PCR.

286 Real time-PCR reveals significant reductions in expression of neut

287 14 days at room temperature, in contrast to PCR that showed significant loss of signal.

288 COVID(pos) patients during the week prior to PCR testing, in addition to anosmia/dysgeusia, constitut

289 totype, and SARS-CoV-2 reverse transcriptase PCR (RT-PCR) results were compared.

290 ays, gel-based PCR and reverse transcriptase PCR, and quantitative real-time PCR (qPCR).

291 ytometry, quantitative reverse-transcriptase PCR (qRT-PCR), and RNA-Seq for PD-1 expression.

292 croarray profiling and reverse transcriptase-PCR.

293 Reverse transcription PCR (RT-PCR) and sequencing revealed that all US isolate

294 luated by quantitative reverse transcription-PCR (qRT-PCR) subsequently confirmed QS upregulation wit

295 lytic stage, real-time reverse transcription-PCR (RT-PCR) assays remain the molecular test of choice

296 Quantitative reverse transcription-PCR and tandem mass spectrometry protein analysis were u

297 ic flow cytometry, and reverse transcription-PCR.

298 Selected strains underwent PCR-based detection of phylogroups, sequence types (STs)

299 The primary outcome was PCR-confirmed incident SARS-CoV-2 infection among person

300 6, 2020 for hospitalized pregnant women with PCR-confirmed SARS-CoV-2 infection and room air oxygen s