ライフサイエンスコーパス: POPE

1 POPE (Phylogeny, Ortholog and Paralog Extractor) provide

2 POPE allows the scientist to explore and understand the

3 POPE bilayers simulated at areas smaller than optimal ex

4 POPE is applied two biological simulators: a fast and st

5 with a free energy of -10.9 kcal/mol in 2:1 POPE/POPG lipid bilayers.

6 tside, 2:1 mol:mol POPE:POPS inside (SMo/2:1 POPE:POPSi) the outer leaflet SM formed an ordered state

7 -oleoyl-phosphatidylglycerol (POPG), and 3:1 POPE:POPG were also conducted, and the presence of anion

8 layers composed of 4:1 POPC:cholesterol, 4:1 POPE:cholesterol, 3:1 POPC:1-palmitoyl-2-oleoyl-phosphat

9 The data collected reveal that 1) POPE is anionic and not neutral at pH 7.4; 2) high-anion

10 analogous mixtures of [(2)H(31)]16:0-18:1PE (POPE*) or [(2)H(31)]16:0-22:6PE (PDPE*) with egg SM and

11 -glycero-3-phosphoethanolamine (16:0-18:1PE, POPE) or 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phos

12 ethanolamine (POPE); and 3) a mixture of 75% POPE, 20% 1-palmitoyl 2-oleoyl-phosphatidylglycerol (POP

13 cholesterol into J5 LPS/POPE liposomes at a POPE:cholesterol molar ratio of 1:0.15 blocked human SP-

14 roteins FhuA, LamB, NanC, OmpA and OmpF in a POPE/POPG (3:1) bilayer were performed to characterise t

15 hough there are differences between POPC and POPE/POPG bilayers, in both cases the toxin forms favora

16 m molecular dynamics simulations in POPC and POPE/POPG membranes.

17 tures of the monounsaturated lipids SOPC and POPE as a function of temperature and composition by NMR

18 Remarkably, when POPG/TOCL and POPE/POPG liposomes were co-incubated, Mac1 did not indu

19 ces to the lipid (31)P: 4.0-6.5 A in anionic POPE/POPG membranes and 6.5-8.0 A in zwitterionic POPC m

20 he inactive TPA4 in bacteria-mimetic anionic POPE/POPG bilayers and compared them with the wild-type

21 s with zwitterionic (POPC) and with anionic (POPE/POPG) lipid bilayers.

22 Here, liposomes containing AzoPC, POPE, and POPG have been characterized by solid-state NM

23 o or only small coupling differences between POPE and POPG in the presence of any of the cationic pep

24 e used in single-component membranes, binary POPE/POPG (3:1) membranes, and membranes containing one

25 l-2-oleoyl-phospha tidylcholine/cholesterol (POPE/POPC/CHOL) bilayers was measured as a function of P

26 mbrane of S. aureus (POPG/TOCL) and E. coli (POPE/POPG) were lysed at similar concentrations, whereas

27 s, but not B5 LPS/POPE liposomes or control (POPE only) liposomes.

28 for post optimization posterior evaluation (POPE) of simulators.

29 scans showed CHOL has a strong affinity for POPE, comparable to that observed between SM-CHOL.

30 ystalline phases coexist in the peptide-free POPE/POPG membrane, the peptides caused distinct quadrup

31 -incubated, Mac1 did not induce leakage from POPE/POPG liposomes, suggesting a preference toward POPG

32 2-oleoyl-sn-glycerophosphatidylethanolamine (POPE) or 1-palmitoyl-2-docosahexaenoyl-sn-glycerophospha

33 resulted in the formation of microdomains in POPE/POPS monolayers, but only SPM promoted a substantia

34 pectra also implied intimate lipid mixing in POPE/SM/CHOL (1:1:1 mol).

35 orming dipyrenylphosphatidylcholine probe in POPE/POPC mixtures were detected at X(PE) approximately

36 2-oleoyl-L-alpha-phosphatidylethano lam ine (POPE) multilamellar vesicles have been determined fluoro

37 observed with the incorporation of SPM into POPE/POPS membranes was, therefore, attributed to larger

38 ity of J5 LPS/POPE liposomes, but not B5 LPS/POPE liposomes or control (POPE only) liposomes.

39 Incorporation of cholesterol into J5 LPS/POPE liposomes at a POPE:cholesterol molar ratio of 1:0.

40 elittin increased the permeability of J5 LPS/POPE liposomes, but not B5 LPS/POPE liposomes or control

41 00 microg/mL, the permeability of the J5 LPS/POPE membranes increased 4.4-fold (p < 0.02) compared to

42 Homogeneously mixed POPE/POPG membranes should give the same quadrupolar cou

43 nside (SMo/DOPCi) or SM outside, 2:1 mol:mol POPE:POPS inside (SMo/2:1 POPE:POPSi) the outer leaflet

44 POPE and POPG disorder: approximately 80% of POPE partitioned into the ordered phase, whereas all of

45 ured to determine the motional amplitudes of POPE and POPG acyl chains as a function of temperature.

46 R spectra of the perdeuterated sn-1 chain of POPE-d(31) increased by >50% upon addition of equimolar

47 components, were determined as a function of POPE mole fraction (X(PE)) at 22.4 degrees C.

48 OPE) mixtures were measured as a function of POPE mole fraction (X(PE)) at 23 degrees C.

49 CHOL) bilayers was measured as a function of POPE-to-phospholipid mole ratio (X(PE)) and cholesterol-

50 evaluated in reconstituted bilayers made of POPE/POPS (3.3:1), or POPE/POPS with an added 20% of eit

51 At 30 degrees C, i.e., above the Tm of POPE and POPC, deviations, or dips, as well as local dat

52 rted hexagonal (L - HII) phase transition of POPE.

53 te influence on the two-phase transitions of POPE brings a three-phase coexistence line when the two

54 or palmitoyl-oleoylphosphatidylethanolamine (POPE).

55 ential scanning calorimetric measurements on POPE/POPC liposomes with increasing X(PE) indicated that

56 tuted bilayers made of POPE/POPS (3.3:1), or POPE/POPS with an added 20% of either SPM (3.3:1:1), CER

57 ine and palmitoyloleoylphosphatidylglycerol (POPE/POPG) bilayers] or the red blood cell membrane [neu

58 leoyl-PC (POPC) and 1-palmitoyl-2-oleoyl-PE (POPE) binary mixtures as a function of the POPE mole fra

59 -2-oleoyl-PC (POPC)/1-palmitoyl-2-oleoyl-PE (POPE) mixtures were measured as a function of POPE mole

60 Chain-perdeuterated POPE and POPG are used in single-component membranes, bi

61 leoyl-sn-glycero-3-phosphatidylethanolamine (POPE) and anionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phos

62 -oleyl-3-n-glycero-phosphatidylethanolamine (POPE) lipid bilayer and its structural properties calcul

63 palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE) and 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC

64 palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE) in the inner leaflet.

65 palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE) lipid bilayers.

66 palmitoyl 2-oleoyl-phosphatidylethanolamine (POPE); and 3) a mixture of 75% POPE, 20% 1-palmitoyl 2-o

67 of palmitoyloleoyl-phosphatidylethanolamine (POPE) and palmitoyloleoyl-phosphatidylglycerol (POPG) li

68 holesterol with PO phosphatidylethanolamine (POPE) and PO phosphatidylserine (POPS) or with brain PE

69 l-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), using differential scanning calorimetry, and sequ

70 l-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), with or without rough Escherichia coli LPS (J5),

71 or 1-palmitoyl 2-oleoyl phosphoethanolamine (POPE).

72 t not an oleic acid-containing phospholipid (POPE).

73 of polar headgroup heterogeneity (e.g., POPC/POPE).

74 In a mixture [POPC/POPE/POPS/PI/ergosterol (30:20:5:20:25)] which mimicked

75 Interaction was also observed with POPC:POPE:cholesterol lipid vesicles (LUV) in equilibrium dia

76 As a Bayesian inference procedure, POPE provides a rigorous framework for the analysis of t

77 In comparison, TAT rigidified POPE and POPG similarly in the binary membrane at ambien

78 less for PDPE-d(31)/SM/CHOL (1:1:1 mol) than POPE-d(31)/SM/CHOL (1:1:1 mol).

79 IB549, caused even larger differences in the POPE and POPG disorder: approximately 80% of POPE partit

80 the peptides by assessing the changes in the POPE/POPG bilayer affinity (DeltaDeltaG) resulting from

81 four to five subunits observed by NMR in the POPE/POPG bilayer.

82 minated the phase separation observed in the POPE/POPS bilayer.

83 Penetratin maintained the POPE order but disordered POPG, suggesting moderate doma

84 eptide PG-1 ordered approximately 40% of the POPE lipids while disordering POPG.

85 (POPE) binary mixtures as a function of the POPE mole fraction (X(PE)) using fluorescence and Fourie

86 liquid-crystalline domain boundaries at this POPE content.

87 The addition of CHL to POPE/POPS eliminated the phase separation observed in th

88 Close proximity of CHOL to POPE even in the presence of SM is indicated.

89 d by >50% upon addition of equimolar CHOL to POPE-d(31)/SM (1:1 mol) bilayers.

90 PM but without its bulky polar head group to POPE/POPS, was without effect, as was the addition of CH

91 ayers, while this interaction tightened when POPE (1-hexadecanoyl-2-(9-Z-octadecenoyl)-sn-glycero-3-p