ライフサイエンスコーパス: PepT1

1 induced expression of peptide transporter 1 (PEPT1).

2 etitively binding to the external surface of PepT1.

3 nsmembrane domain (TMD) topological model of PEPT1.

4 etitively binding to the external surface of PepT1.

5 H(2)O or with RNA encoding SGLT1, NKCC2, or PepT1.

6 edly, some dipeptides were not substrates of PEPT1.

7 tides could not bind to or be transported by PEPT1.

8 essed by incorporating amino acids to target PepT1/2 active transport, followed by in vivo hydrolysis

9 were unimpaired in macrophages deficient in PepT1, a peptide transporter previously implicated in MD

10 in animal models of type 2 diabetes/obesity, PepT1 activity and expression were markedly reduced.

11 A 7-day rosiglitazone treatment increased PepT1 activity and prevented the 2-fold HFD-induced redu

12 Metformin alone did not modify PepT1 activity but counteracted rosiglitazone-induced Pe

13 betic drugs, rosiglitazone and metformin, on PepT1 activity/expression in a murine diet-induced obesi

14 Studies of expression and function of PepT1 along the crypt-villus axis demonstrated that this

15 taste receptors to coordinate regulation of PepT1 and apical GLUT2 reciprocally through a common ent

16 ip between the H(+)/oligopeptide transporter PepT1 and apical GLUT2, reflecting the fact that traffic

17 UT2, reflecting the fact that trafficking of PepT1 and GLUT2 to the apical membrane is inhibited and

18 dicates tht KPV is transported into cells by PepT1 and might be a new therapeutic agent for IBD.

19 distinct properties of PepT2, compared with PepT1 and other ion-coupled transporters.

20 PepT1 and PepT2 are major facilitator superfamily (MFS)

21 C15 family of proton-coupled solute carriers PepT1 and PepT2 play a central role in human physiology

22 lly target two peptide transporters (namely, PEPT1 and PEPT2) upregulated in several tumor cells.

23 he proton-coupled oligopeptide transporters, PepT1 and PepT2, have been successfully targeted using t

24 e intestinal and renal peptide transporters (PEPT1 and PEPT2, respectively) from different animal spe

25 PepT1 and PepT2, the mammalian proton coupled peptide tr

26 In mammals, peptide transport occurs via PepT1 and PepT2, which belong to the proton-dependent ol

27 mammalian peptide transport as catalysed by PepT1 and PepT2.

28 es for the H(+)-coupled peptide transporters PEPT1 and PEPT2.

29 e recognized by OCTN2, did not interact with PEPT1 and PEPT2.

30 intestinal H+-coupled peptide cotransporter PepT1 and recorded the dependence of transient charge mo

31 es of the mammalian oligopeptide transporter PepT1 and the Na(+)- and K(+)-coupled epithelial and neu

32 oth Xenopus Laevis oocytes expressing rabbit PepT1 and through PepT1 in rat renal brush border membra

33 oth Xenopus laevis oocytes expressing rabbit PepT1 and through PepT1 in rat renal brush border membra

34 that transport of the dipeptide Trp-Gly via PepT1 and transport of Na+ and K+ via EAAC1 across the o

35 Thus, R282E-Pept1 appears to have been changed from a proton-driven

36 ncluded that peptide-evoked charge fluxes of PepT1 are entirely due to H+ movement.

37 This molecule is not translocated through PepT1 as measured both by direct HPLC analysis in PepT1-

38 This molecule is not translocated through PepT1 as measured both by direct HPLC analysis in PepT1-

39 ectrode voltage clamping revealed that R282E-PepT1 behaved as a peptide-gated non-selective cation ch

40 ort of protons and peptides of the wild-type PepT1 but also creates a peptide-gated cation channel in

41 e maximum velocity for dipeptide uptake (via PepT1), but they act by different mechanisms.

42 Collectively, our results suggest that PepT1 contributes to maintain balance of homeostasis and

43 PepT1 did not display the substrate-gated anion conducta

44 intestinal H(+)-coupled peptide transporter PepT1, displays a broad substrate specificity and accept

45 amma agonist compensated for the HFD-induced PepT1 down-regulation, whereas metformin reversed rosigl

46 as measured both by direct HPLC analysis in PepT1-exp ressing oocytes and indirectly by its failure

47 We found that KPV acts via PepT1 expressed in immune and intestinal epithelial cell

48 The oligopeptide transporter PepT1 expressed in inflamed colonic epithelial cells tra

49 as measured both by direct HPLC analysis in PepT1-expressing oocytes and indirectly by its failure t

50 Furthermore, metformin decreased PepT1 expression (mRNA and protein) and its transport ac

51 stigated the long term leptin treatments, on PepT1 expression and activity in Caco2-BBE cells, and be

52 EPEC induced PepT1 expression and activity in HT29-Cl.19A cells by in

53 ts revealed that C rodentium induced colonic PepT1 expression and that, compared with their wild-type

54 Induction of PepT1 expression by EPEC required the transcription fact

55 PepT1 expression reduced binding of EPEC to lipid rafts,

56 he effects of pathogenic bacteria on colonic PepT1 expression together with its functional consequenc

57 chanism underlying the regulation of colonic PepT1 expression under pathologic conditions and reveal

58 PepT1 expression was high in the apical region of duoden

59 e human intestinal oligopeptide transporter (PEPT1) facilitates the absorption of dipeptides, tripept

60 nd crypts samples, we found that ablation of PepT1 further directly or indirectly altered expression

61 arginine 282 with a glutamate residue (R282E-PepT1) gave a protein at the plasma membrane of Xenopus

62 Here, a novel PepT1 gene product has been identified in the rat pineal

63 We propose that PepT1 has a single proton binding site that is symmetric

64 Expression of the di/tripeptide transporter PepT1 has been observed in the colon under inflammatory

65 roton-coupled di- and tripeptide transporter PepT1 has been shown by site-directed mutagenesis to be

66 of 12 membrane-spanning domain transporters; PepT1 has proton/peptide cotransport activity and is sel

67 th and expression of the peptide transporter PepT1 in adults with or without SBS.

68 logic conditions and reveal a novel role for PepT1 in host defense via its capacity to modulate bacte

69 ns-stimulate labelled peptide efflux through PepT1 in oocytes and in renal membrane vesicles.

70 s-stimulate labelle d peptide efflux through PepT1 in oocytes and in renal membrane vessicle s.

71 oocytes expressing rabbit PepT1 and through PepT1 in rat renal brush border membrane vesicles.

72 oocytes expressing rabbit PepT1 and through PepT1 in rat renal brush border membrane vesicles.

73 Up-regulation of colonic PepT1 in SBS may adaptively improve accrual of malabsorb

74 lacetic acid (epithelial peptide transporter PepT1 inhibitor), 3,4-dihydroxyphenylacetic acid (dopami

75 PepT1 is a di/tripeptide transporter normally expressed

76 PepT1 is abundant along the small-bowel brush border in

77 PepT1 is an intestinal epithelial apical membrane transp

78 In the jejunum, PepT1 is particularly enriched in the well-differentiate

79 In addition, a substrate binding model for PepT1 is proposed.

80 The oligopeptide transporter 1, PepT1, is a member of the Slc15 family of 12 membrane-sp

81 An oligopeptide transporter, PepT1, is transcriptionally upregulated by certain dieta

82 with previous data on the ion dependence of PepT1, it can therefore be concluded that peptide-evoked

83 the COOH terminus of a warm-adapted (rabbit) PEPT1, it conferred cold adaptation to the receiving pro

84 Ac-Phe-NH2 had a very weak interaction with PepT1 (Ki = 16.8+/-5.64 mM); neither Phe nor Phe-NH2 int

85 yr and Ac-Phe-Tyr-NH2 interacted weakly with PepT1 (Ki = 8.41+/-0.11 and 9.97+/-4.01 mM, respectively

86 In this study, we observed that PepT1 knockout (KO) mice exhibited reduced body weight a

87 We found that PepT1 KO altered the distribution of miRNAs along the cr

88 along the crypt-villi axis in the jejunum of PepT1 KO mice.

89 ch activated PKC betaII and decreased apical PepT1 levels and absorption of the hydrolysis-resistant

90 ransport of VLPVPQK suggested involvement of PepT1 like transporters/SOPT2 while BCM 5, its hydrolyti

91 ver, we previously demonstrated that colonic PepT1 may be expressed during chronic inflammation.

92 whether the KPV anti-inflammatory effect is PepT1-mediated in intestinal epithelian and immune cells

93 while GLUT5-mediated fructose transport and PepT1-mediated peptide transport were substantially redu

94 ivity but counteracted rosiglitazone-induced PepT1-mediated transport.

95 eptidyl peptidase IV and peptide transporter PepT1 messenger RNA levels were determined before and af

96 ation of mucosal dipeptidyl peptidase IV and PepT1 messenger RNA.

97 However, the abundance of PepT1 mRNA in the colon of SBS patients was more than 5-

98 The PepT1-NOD2 signaling pathway is involved in aggravation

99 studied to determine the involvement of the PepT1-NOD2 signaling pathway.

100 gh most of the peptides tested could bind to PEPT1, not all were substrates.

101 reverse trans-stimulation through expressed PepT1 of labelled peptid efflux induced by unlabelled pe

102 reverse trans-stimulation through expressed PepT1 of labelled peptide efflux induced by unlabelled p

103 We recently exhibited that PepT1 plays an important role in multiple biological fun

104 The intestinal H(+)/peptide cotransporter 1 (PepT1) plays a major role in nitrogen supply to the body

105 show that at physiological pH (pHo 5.5-6.0) PepT1 prefers neutral and acidic peptides.

106 al 3 membrane-spanning domains of intestinal PepT1 protein, with 3 additional N-terminal residues.

107 performed using cold KPV as a competitor for PepT1 radiolabelled substrate or using [(3)H]KPV to dete

108 Its homologues in mammals, PEPT1 (SLC15A1) and PEPT2 (SLC15A2), not only transport

109 d by the proton-coupled peptide transporters PepT1 (SLC15A1) and PepT2 (SLC15A2).

110 permease), shows a high similarity to human PepT1 (SLC15A1) in terms of ligand selectivity and trans

111 aporin (AQP) 3, AQP4, peptide transporter 1 (PepT1), sodium/glucose co-transporter-1 (SGLT-1), potass

112 In addition, peptide transport by R282E-PepT1 still induced depolarization as measured by microe

113 at contain a diaminophilic acid, such as the PepT1 substrate Tri-DAP.

114 in contrast to the low-affinity transporter PepT1 that couples transport of one peptide to one H+.

115 (Chionodraco hamatus) peptide transporter 1 (PEPT1), the first transporter cloned from a vertebrate l

116 n of nutrient transporters (SMCT2, MCT1, and PepT1), tight junction proteins (OCL and ZO-1), antioxid

117 We investigated the contribution of PepT1 to intestinal inflammation in mouse models of coli

118 compared with their wild-type counterparts, PepT1 transgenic mice infected with C rodentium exhibite

119 Wild-type and PepT1 transgenic mice or cultured colonic tissues derive

120 In other words, PepT1 transport activity plays an important role in cont

121 tudies, rosiglitazone treatment up-regulated PepT1 transport activity with concomitant induction of S

122 diabetic drugs exert opposite effects on the PepT1 transport function probably through direct action

123 r results identify key features required for PEPT1 transport in contrast to most previously described

124 revented the 2-fold HFD-induced reduction in PepT1 transport.

125 stigate the structural features required for PEPT1 transport.

126 gether with their potential active uptake by PEPT1 transporter, intestinal permeability, and metaboli

127 this binding mode also applies to the human PepT1 transporter.

128 illus width were measured, and expression of PepT1 was determined by Northern blotting, in situ hybri

129 parameters on extracellular pH (pHo), rabbit PepT1 was expressed in Xenopus laevis oocytes and used f

130 he wild type, the rate of transport by R282E-PepT1 was independent of the extracellular pH level, and

131 Slc15a1, a peptide transporter also known as Pept1, was predominantly present in peritubular myoid ce

132 the mammalian intestinal peptide transporter PepT1 were investigated, using the Xenopus laevis expres

133 nal peptide Phe-Tyr-NH2 also interacted with PepT1 with a relatively high affinity (Ki = 0.94+/-0.38

134 mM); neither Phe nor Phe-NH2 interacted with PepT1 with measurable affinity.