ライフサイエンスコーパス: Poliota

1 Poliota can also extend a tandem mispair, especially whe

2 Poliota cannot initiate synthesis on a nicked DNA substr

3 Poliota is highly unusual in that it incorporates nucleo

4 Poliota is highly unusual in that it possesses a high fi

5 Poliota monoubiquitination remains unchanged after cells

6 Here, we show that although Poliota, Poleta, and Polkappa are all able to form a cov

7 er error-prone TLS polymerases (Polkappa and Poliota) after UV irradiation.

8 three DNA polymerases, Poleta, Polkappa, and Poliota, which are able to promote replication through D

9 WRN and the TLS Pols, Poleta, Polkappa, and Poliota.

10 base and that when T is the templating base, Poliota accommodates the wobble base pair better than th

11 oration opposite different template bases by Poliota, we have carried out pre-steady-state kinetic an

12 The mode of PCNA binding by Poliota is quite unlike that in Poldelta, where multisit

13 our kinetic and structural studies show how Poliota maintains discrimination between correct and inc

14 ne, here we examine the proficiency of human Poliota and Polkappa to synthesize past stereoisomers of

15 s mediated by the sequential action of human Poliota and Polkappa, in which Poliota incorporates the

16 base with the incoming nucleotide, and (ii) Poliota can accommodate a minor-groove-adducted template

17 DNA polymerase iota (Poliota) belongs to the Y-family of DNA polymerases that

18 stal structure of human DNA polymerase iota (Poliota) has shown that it differs from all known Pols i

19 Human DNA polymerase iota (Poliota) is a member of the Y family of DNA polymerases

20 DNA polymerase iota (Poliota) is a member of the Y family of DNA polymerases,

21 we determine how human DNA polymerase-iota (Poliota) promotes error-free replication across 1-MeA.

22 ta/Polzeta is consistent with the ability of Poliota to incorporate nucleotide opposite epsilondA by

23 Interestingly, we find that acetylation of Poliota significantly and specifically increases in resp

24 As we have not observed acetylation of Poliota's closest paralogue, DNA polymerase eta (Poleta)

25 We show here that the sequential action of Poliota and Polkappa promotes efficient and error-free s

26 The requirement of Poliota/Polzeta is consistent with the ability of Poliot

27 suggest that an important biological role of Poliota and Polkappa is to act sequentially to carry out

28 The role of Poliota in lesion bypass, however, has remained unclear.

29 o be determined, and separate function(s) of Poliota.

30 commodated differently in the active site of Poliota dependent upon the template base and that when T

31 ctivity, we determined ternary structures of Poliota bound to template 1-MeA and incoming dTTP or dCT

32 0 amino acid substitutions have no effect on Poliota's ability to interact with Rev1.

33 esis by human Pols eta, iota, or kappa, only Poliota is able to incorporate nucleotides opposite the

34 al studies, in the Poliota/Polkappa pathway, Poliota inserts a nucleotide (nt) opposite 3-dMeA and Po

35 dicate that in the Poliota/Poltheta pathway, Poliota would carry out nucleotide insertion opposite 1-

36 is indispensable for TLS mediated by Poleta, Poliota, and Polkappa but is not required for TLS by Pol

37 1) in Rev1-dependent TLS by Y family Poleta, Poliota, or Polkappa and show that WRN, WRNIP1, and Rev1

38 ispensable scaffolding component for Poleta, Poliota, and Polkappa, which function in TLS in highly s

39 ee different pathways dependent upon Poleta, Poliota, and Polnu, respectively; and that TLS by all th

40 The human Y-family DNA polymerases, Poliota, Poleta, and Polkappa, function in promoting rep

41 Like all other Y-family polymerases, Poliota interacts with proliferating cell nuclear antige

42 ming Hoogsteen base pair with the T residue, Poliota would carry out TLS opposite 1-MeA, the ability

43 We show here that Poliota incorporates a C opposite this adduct with nearl

44 Steady state kinetic analyses indicate that Poliota is 100 fold more efficient in incorporating the

45 Steady-state kinetic analyses indicated that Poliota and Poltheta insert the correct nt T opposite 3-

46 te bases, we considered the possibility that Poliota differs from other DNA polymerases in not being

47 Here, we report that Poliota also interacts with the p300 acetyltransferase a

48 Here we show that Poliota interacts with PCNA via only one of its conserve

49 As inferred from biochemical studies, in the Poliota/Polkappa pathway, Poliota inserts a nucleotide (

50 Our biochemical studies indicate that in the Poliota/Poltheta pathway, Poliota would carry out nucleo

51 via three different pathways, dependent upon Poliota/Polkappa, Poltheta, and Polzeta.

52 srupts Watson-Crick base pairing, occurs via Poliota/Polzeta-, Rev1-, and Poltheta-dependent pathways

53 e validity of this idea, we examined whether Poliota could incorporate nucleotides opposite the gamma

54 polymerases (Pols) iota and kappa, in which Poliota incorporates either pyrimidine opposite gamma-HO

55 nthesis through the HNE-dG adducts, in which Poliota incorporates the nucleotide opposite the lesion

56 tion of human Poliota and Polkappa, in which Poliota incorporates the nucleotide opposite the lesion

57 gue, DNA polymerase eta (Poleta), with which Poliota shares many functional similarities, we believe