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1 erium tuberculosis and from three species of Pyrococcus.
2 NAD+ ligases in two Thermococcus species and Pyrococcus abyssi and an ATP/ADP ligase in Aeropyrum per
4 neutral sodium/proton antiporter PaNhaP from Pyrococcus abyssi at 3.2 A, and have determined its stru
5 errupts the DNA polymerase II DP2 subunit in Pyrococcus abyssi can be overexpressed and purified as a
6 errupts the DNA polymerase II DP2 subunit in Pyrococcus abyssi can be overexpressed in Escherichia co
7 from the deep sea hyperthermophilic archaeon Pyrococcus abyssi demonstrate the existence of carbamoyl
8 structure of the archaeal MCM helicase from Pyrococcus abyssi in its single octameric ring assembly.
11 tion NMR structures of the hyperthermophilic Pyrococcus abyssi PolII intein, which has a noncanonical
12 structures of the zymogens of two of these (Pyrococcus abyssi proabylysin and Methanocaldococcus jan
14 transfer RNA synthetase from the thermophile Pyrococcus abyssi that forms complementary van der Waals
15 fide origin of replication was reported was Pyrococcus abyssi, where a single origin was identified.
16 rupting the DNA polymerase II DP2 subunit in Pyrococcus abyssi, which has a C-terminal glutamine, is
18 of two organisms corresponding to the genus Pyrococcus and three groups corresponding to the genus T
19 oncluded that the structural alternations in Pyrococcus Fd relative to other hyperthermostable Fds ar
20 n with protein disulfide oxidoreductase from Pyrococcus furiosis, we describe a new class of protein
21 of RPP21 from the hyperthermophilic archaeon Pyrococcus furiosus ( Pfu) using conventional and parama
22 ophilum (optimal growth at 55 degrees C) and Pyrococcus furiosus (100 degrees C) are homo-dimeric enz
23 s) that have been annotated in the genome of Pyrococcus furiosus (optimal growth temperature, 100 deg
25 of the rubredoxins from the hyperthermophile Pyrococcus furiosus (Pf) and the mesophile Clostridium p
26 s of Rds from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) and the mesophilic bacterium Cl
27 se (POR) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) catalyzes the final oxidative s
28 xin (Fd) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) have been characterized by (1)H
29 xin (Fd) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) possesses several unique proper
30 distance to the [Fe(SCys)(4)] center in the Pyrococcus furiosus (Pf) Rd crystal structure compared t
32 ter of the hyperthermophilic marine archaea, Pyrococcus furiosus (Pf), we have determined the locatio
35 ferritin from the hyperthemophilic bacterium Pyrococcus furiosus (PfFt), have been used as models for
36 nding studies with a mutant of ferritin from Pyrococcus furiosus (PfFtn) in which self-assembly was a
37 crystal structure of a MATE transporter from Pyrococcus furiosus (PfMATE) in the long-sought-after in
41 eptidase from the hyperthermophilic archaeon Pyrococcus furiosus (PfMetAP-II; EC 3.4.11.18) has been
42 structures of apo- and holorubredoxins from Pyrococcus furiosus (PfRd) and Clostridium pasteurianum
43 rks in rubredoxins from the hyperthermophile Pyrococcus furiosus (PfRd), and its mesophilic analogue
44 luble [NiFe]-hydrogenase: hydrogenase I from Pyrococcus furiosus (PfSHI), which grows optimally near
46 allosteric regulation existing in TrpS from Pyrococcus furiosus (PfTrpS), and how the allosteric con
47 he thermostable family B DNA polymerase from Pyrococcus furiosus (Pfu Pol) contains sensitive determi
48 on the POP of the hyperthermophilic archaeon Pyrococcus furiosus (Pfu) 85 degrees C in both H(2)O and
49 P holoenzyme from the thermophilic archaeon Pyrococcus furiosus (Pfu) and furthered our understandin
50 ities associated with Type III-B immunity in Pyrococcus furiosus (Pfu) are regulated by target RNA fe
51 f-replication (CSR), we evolved a version of Pyrococcus furiosus (Pfu) DNA polymerase that tolerates
53 ng strategy to pinpoint the binding sites of Pyrococcus furiosus (Pfu) L7Ae on its cognate RNase P RN
54 ronounced unwinding of primer-templates with Pyrococcus furiosus (Pfu) polymerase-DNA complexes conta
56 urthermore, an N-terminal deletion mutant of Pyrococcus furiosus (Pfu) RPP29 that is defective in ass
57 ered a thermostable enzyme from the archaeon Pyrococcus furiosus (Pfu), which increases yields of PCR
58 mutants of the family B DNA polymerase from Pyrococcus furiosus (Pfu-Pol), with superb performance i
61 ics of rubredoxins from the hyperthermophile Pyrococcus furiosus (RdPf) and the mesophile Clostridium
63 (Topt = 37 degrees C) and hyperthermophilic Pyrococcus furiosus (Topt = 95 degrees C) were recorded
64 e we show that purified Mre11 and Rad50 from Pyrococcus furiosus act cooperatively with HerA and NurA
65 cer integration in vitro using proteins from Pyrococcus furiosus and demonstrate that Cas1 and Cas2 a
66 50 homologues from the thermophilic archaeon Pyrococcus furiosus and demonstrate that the two protein
67 and human clamp loaders, and the two protein Pyrococcus furiosus and Methanobacterium thermoautotroph
68 Divergence of the hyperthermophilic Archaea, Pyrococcus furiosus and Pyrococcus horikoshii, was asses
70 mate dehydrogenase from the hyperthermophile Pyrococcus furiosus and the comparison of this structure
71 bredoxin from the hyperthermophilic archaeon Pyrococcus furiosus and the mesophilic bacterium Clostri
72 te dehydrogenases from the hyperthermophiles Pyrococcus furiosus and Thermococcus litoralis whose opt
75 have applied directed evolution to TrpB from Pyrococcus furiosus and Thermotoga maritima to generate
76 cale applications on Caenorhabditis elegans, Pyrococcus furiosus and three cyanobacterial genomes are
78 cryoEM), we have solved the structure of the Pyrococcus furiosus archaellum filament at 4.2 A resolut
79 Pyrococcus horikoshii (PH1704) and PfpI from Pyrococcus furiosus are members of a class of intracellu
82 protein-translocating channel SecYEbeta from Pyrococcus furiosus at 3.1-A resolution suggests a mecha
84 protein from the hyperthermophilic archaeon Pyrococcus furiosus belongs to the Lrp/AsnC family of tr
86 systems, Cmr eliminates plasmid invaders in Pyrococcus furiosus by a mechanism that depends on trans
87 l extracts of the hyperthermophilic archaeon Pyrococcus furiosus by its ability to hydrolyze N-acetyl
89 dase A, but instead resembles neurolysin and Pyrococcus furiosus carboxypeptidase--zinc metallopeptid
93 from cells of the hyperthermophilic archaeon Pyrococcus furiosus contain high hydrogenase activity (9
94 the proteolytic, hyperthermophilic archaeon Pyrococcus furiosus contain high specific activity (11 U
95 annotation of the hyperthermophilic archaeon Pyrococcus furiosus contained 2,065 open reading frames
97 The thermostable DNA polymerase derived from Pyrococcus furiosus designated Pfu has the highest fidel
100 The genome of the hyperthermophile archaeon Pyrococcus furiosus encodes two transcription factor B (
101 information has been available only for the Pyrococcus furiosus enzyme (PfMre11), the conserved and
102 se extensions of stabilizing interactions in Pyrococcus furiosus Fd, however, lead to strong destabil
105 ication of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.
106 the nature of the residue at position 14 in Pyrococcus furiosus ferredoxin is an important determina
107 e conformationally dynamically heterogeneous Pyrococcus furiosus ferredoxin with an intact disulfide
110 in the AT-rich Methanococcus jannaschii and Pyrococcus furiosus genomes efficiently detects both kno
116 ave recently shown that the hyperthermophile Pyrococcus furiosus has an extraordinarily high capacity
117 s of SOR from the hyperthermophilic archaeon Pyrococcus furiosus have been determined in the oxidized
118 of the archaeal family-B DNA polymerase from Pyrococcus furiosus have been investigated, illuminating
119 r-2 gene from the hyperthermophilic archaeon Pyrococcus furiosus having homology to bacterial and euk
122 determined the crystal structure of PGI from Pyrococcus furiosus in native form and in complex with t
125 rredoxin from the hyperthermophilic archaeon Pyrococcus furiosus is a monomeric protein (7.5 kDa) tha
127 nase (SHI) of the hyperthermophilic archaeon Pyrococcus furiosus is an NADP(H)-dependent heterotetram
130 tors: Ptr-H10, fusing the effector domain of Pyrococcus furiosus LrpA, and Ptr-H16, fusing the P. fur
131 We determined the crystal structure of the Pyrococcus furiosus MCM N-terminal domain hexamer bound
132 in DNA double-strand break repair, we report Pyrococcus furiosus Mre11 crystal structures, revealing
133 scattering (SAXS) and crystal structures of Pyrococcus furiosus Mre11 dimers bound to DNA with mutat
136 M N-terminal domain of the archaeal organism Pyrococcus furiosus occurs specifically in the hexameric
138 led MATE from the hyperthermophilic archaeon Pyrococcus furiosus Pairs of spin labels monitoring the
141 Transcriptional and enzymatic analyses of Pyrococcus furiosus previously indicated that three prot
143 Here we design structure-based mutations in Pyrococcus furiosus Rad50 to alter protein core plastici
145 of operons in the hyperthermophilic archaeon Pyrococcus furiosus represents an important step to unde
146 the 2.3 A crystal structure of mre11-3 from Pyrococcus furiosus revealed an active site structure wi
148 on Zn(II)-, Ga(III)-, and Ge(IV)-substituted Pyrococcus furiosus rubredoxin demonstrate that the log
149 (Trp) solvation dynamics in water and in the Pyrococcus furiosus rubredoxin protein, including the na
150 static solvent-accessible amide hydrogens of Pyrococcus furiosus rubredoxin range from near the diffu
151 l-atom molecular-dynamics simulations of the Pyrococcus furiosus SecYE, which was determined to be in
152 identification and characterization of SurR, Pyrococcus furiosus sulphur (S(0)) response regulator.
153 perties of the oxidized and reduced forms of Pyrococcus furiosus superoxide reductase (SOR) as a func
154 ction at the mononuclear iron active site of Pyrococcus furiosus superoxide reductase (SOR) through t
155 he site of base exchange; truncated forms of Pyrococcus furiosus TGT retain their specificity for gua
157 n initiation complexes have been formed with Pyrococcus furiosus transcription factors (TBP and TFB1)
158 cations with larger quantities (100 ng) of a Pyrococcus furiosus tryptic digest, but with mass-limite
160 eductase from the hyperthermophilic anaerobe Pyrococcus furiosus uses electrons from reduced nicotina
161 r E17 to Q in the soluble hydrogenase I from Pyrococcus furiosus using site directed mutagenesis.
163 n from the hyperthermophilic archaebacterium Pyrococcus furiosus was examined by a hydrogen exchange
164 cosidase from the hyperthermophilic archaeon Pyrococcus furiosus was recombinantly produced in Escher
165 ock protein (sHSP) from the hyperthermophile Pyrococcus furiosus was specifically induced at the leve
166 l-II family DNA polymerase from the archaeon Pyrococcus furiosus with the aim of improving ddNTP util
167 f the type-I (Escherichia coli) and type-II (Pyrococcus furiosus) MetAPs in the presence of the react
168 ironment, such as hyperthermophilic archaea (Pyrococcus furiosus), are significantly more compact and
172 found that the hyperthermophilic archaeaon, Pyrococcus furiosus, actively incorporates DNA fragments
174 urified from the hyperthermophilic archaeon, Pyrococcus furiosus, an organism that grows optimally at
175 l element for the hyperthermophilic archaeon Pyrococcus furiosus, and many of its iron-containing enz
176 hia coli, Artemisia tridentata (sage brush), Pyrococcus furiosus, and Methanobacter thermautotrophicu
177 he metabolite was isolated from the organism Pyrococcus furiosus, and structurally characterized thro
179 (7.5 kDa) of the hyperthermophilic archaeon, Pyrococcus furiosus, contains a single [4Fe-4S]1+,2+ clu
180 PF0610, a protein from the hyperthermophile Pyrococcus furiosus, has homologues only in other archae
181 protein from the hyperthermophilic archaeon Pyrococcus furiosus, have been determined in the native
182 irst characterized from the hyperthermophile Pyrococcus furiosus, it is unique to the archaeal order
183 hermus thermophilus, Archaeoglobus fulgidus, Pyrococcus furiosus, Methanococcus jannaschii, and Metha
184 x cleaves an endogenous complementary RNA in Pyrococcus furiosus, providing direct in vivo evidence o
187 gen exchange results for the rubredoxin from Pyrococcus furiosus, the acidity of these amides was cal
188 etAP from E. coli and the type-II MetAP from Pyrococcus furiosus, the type-I MetAP can be selectively
189 the N-terminal DNA binding domain of Phr of Pyrococcus furiosus, these results suggest that HSR1 and
190 cted 50 representative proteins, mostly from Pyrococcus furiosus, to this pipeline and found that 30
191 lucanase from the hyperthermophilic archaeon Pyrococcus furiosus, was cloned and expressed in Escheri
194 hat confers on a microorganism (the archaeon Pyrococcus furiosus, which grows optimally on carbohydra
195 ned the structures of a PurP orthologue from Pyrococcus furiosus, which is functionally unclassified,
196 amily B DNA polymerases from archaea such as Pyrococcus furiosus, which live at temperatures approxim
197 tryptophan synthase beta-subunit (TrpB) from Pyrococcus furiosus, which mimics the activation afforde
198 gineered subunit of tryptophan synthase from Pyrococcus furiosus, yielding (2S,3S)-beta-methyltryptop
225 hyperthermophiles Pyrococcus horikoshii and Pyrococcus furiosus; however, the form(s) of sulfur that
226 uated using a preliminary set of orthologous Pyrococcus gene pairs, for which it demonstrates an impr
227 genic-sequence content of the three archaeal Pyrococcus genomes to determine the most highly related
228 Six allelic intein sites are common to both Pyrococcus genomes, and two intein insertions occur in e
229 py on the bacterial transporter Glt(Ph) from Pyrococcus horikoshi to examine conformational changes i
230 of comparing domain graphs of two organisms, Pyrococcus horikoshii (an extremophile) and Haemophilus
231 resolution structure of the transporter from Pyrococcus horikoshii (Glt(Ph)) in steered molecular dyn
233 Our laboratory has recently showed that in Pyrococcus horikoshii (P. horikoshii), the first step us
235 eric serine protease, an oligopeptidase from Pyrococcus horikoshii (PhAAP), revealing a complex, self
236 Herein, we present a structure of NadA from Pyrococcus horikoshii (PhNadA) in complex with IA and sh
239 the crystal structure of the gene product of Pyrococcus horikoshii 999 (PH999), a PZAase, and its com
242 studies of the archeal homologs Glt(Ph) from Pyrococcus horikoshii and Glt(Tk) from Thermococcus koda
243 sulfur-reducing anaerobic hyperthermophiles Pyrococcus horikoshii and Pyrococcus furiosus; however,
244 of the structure of l-lysine complexed with Pyrococcus horikoshii class I LysRS (LysRS1) and homolog
245 ervation between P. furiosus and the related Pyrococcus horikoshii clearly delimited the gene start i
246 as been used to kinetically characterise the Pyrococcus horikoshii DNA adenine methyltransferase.
250 ation by showing that Archease and RtcB from Pyrococcus horikoshii function in tandem, with Archease
252 ase domain of the hyperthermophilic archaeon Pyrococcus horikoshii is strongly regulated by the nativ
253 e variants were selected for analysis of the Pyrococcus horikoshii LysRS1-tRNALys docking model.
254 ning to evaluate the interaction between the Pyrococcus horikoshii Nop5p domain and an L7Ae box C/D R
256 eprogramming the anticodon-binding pocket of Pyrococcus horikoshii ProRS (PhProRS), we were able to i
257 Here, we report two crystal structures of Pyrococcus horikoshii RNA-splicing ligase RtcB in comple
259 reviously yielded a crystal structure of the Pyrococcus horikoshii RtcB protein containing a new prot
260 r dynamics simulations of three forms of the Pyrococcus horikoshii species of NikR including two apo-
261 glutamate transporter homologue Glt(Ph) from Pyrococcus horikoshii suggested that the slow conformati
262 of diphthamide biosynthesis in the archaeon Pyrococcus horikoshii uses a novel iron-sulphur-cluster
263 Recently, the X-ray structure of NadA from Pyrococcus horikoshii was solved to 2.0 A resolution.
264 endoglucanase EGPh from the hypothermophilic Pyrococcus horikoshii was transaminated with pyridoxal-5
265 nes from both Mycobacterium tuberculosis and Pyrococcus horikoshii were cloned, and their protein pro
266 s (Escherichia coli, Heliobacter pylori, and Pyrococcus horikoshii) of NikR reveal large conformation
267 moautotrophicum, Archaeoglobus fulgidus, and Pyrococcus horikoshii) revealed 1326 orthologous sets, o
269 on the homologous sequence from subunit B of Pyrococcus horikoshii, an organism that lacks an actin c
271 nt of a glutamate transporter homologue from Pyrococcus horikoshii, Glt(Ph), which is trapped in the
272 mate transporter homologue from the archaeon Pyrococcus horikoshii, GltPh, showed that distinct trans
273 h, a homotrimeric aspartate transporter from Pyrococcus horikoshii, is an archaeal homolog of mammali
275 tem Glt(Ph), an archaeal EAAT homologue from Pyrococcus horikoshii, limited trypsin proteolysis exper
276 ate transporter homolog from archaebacterium Pyrococcus horikoshii, sodium/aspartate symporter GltPh,
277 hermophilic Archaea, Pyrococcus furiosus and Pyrococcus horikoshii, was assessed by analysis of compl
284 (e.g., Bacillus licheniformis TAKA-term and Pyrococcus kodakaraensis KOD1 alpha-amylases, respective
285 imately twice as much tRNA as did AspRS from Pyrococcus kodakaraensis or Ferroplasma acidarmanus.
287 ADR is not essential for S(0) respiration in Pyrococcus or Thermococcus but appears to participate in
289 cognition of U35 and U36 was confined to the pyrococcus-spirochete grouping within the archaeal branc
290 [encoding O-acetylserine (thiol)-lyase-B] in Pyrococcus spp., Sulfolobus solfataricus, and Thermoplas
291 yperthermophiles Methanothermus fervidus and Pyrococcus strain GB-3a) have been determined by circula
293 el (1.7%) of aspartylation of unfractionated Pyrococcus tRNA compared with that achieved by the wild-
295 n of the TATA box binding protein (TBP) from Pyrococcus woesei (Pw) with an oligonucleotide containin
296 pendage combination used the polymerase from Pyrococcus woesei (Pwo) and the 5'-tBu-SS-CH2-CH2-C [tri
297 from the thermophilic and halophilic archaea Pyrococcus woesei (PwTBP) with an oligonucleotide contai
299 from the halophile/hyperthermophile organism Pyrococcus woesei, is adapted to high concentrations of