ライフサイエンスコーパス: Pyrococcus furiosus

1 lloproteins from an exemplary microorganism (Pyrococcus furiosus).

2 (NfnI) from the hyperthermophillic archaeon Pyrococcus furiosus.

3 sing the thermostable protease isolated from Pyrococcus furiosus.

4 nditions from the hyperthermophilic archaeon Pyrococcus furiosus.

5 NA circles in the hyperthermophilic archaeon Pyrococcus furiosus.

6 ucture of the full-length RAD51 homolog from Pyrococcus furiosus.

7 purified from the hyperthermophilic archaeon Pyrococcus furiosus.

8 nosine, from the hyperthermophilic archaeon, Pyrococcus furiosus.

9 e from the hyperthermophilic archaebacterium Pyrococcus furiosus.

10 to 1.2 A from the hyperthermophilic archaeon Pyrococcus furiosus.

11 frame (ORFs) from the genome of the archaeon Pyrococcus furiosus.

12 P-free Rad50 catalytic domain (Rad50cd) from Pyrococcus furiosus.

13 n compared to that from the hyperthermophile Pyrococcus furiosus.

14 ba histolytica and from the archaebacterium, Pyrococcus furiosus.

15 d a surrogate protein system using RadA from Pyrococcus furiosus.

16 sing Bacillus subtilis, Escherichia coli and Pyrococcus furiosus.

17 purified from the hyperthermophilic archaeon Pyrococcus furiosus.

18 utagenesis in the hyperthermophilic archaeon Pyrococcus furiosus.

19 ere we show that primed adaptation occurs in Pyrococcus furiosus.

20 lfovibrio gigas, Desulfovibrio vulgaris, and Pyrococcus furiosus.

21 t in Mbh from the hyperthermophilic archaeon Pyrococcus furiosus.

22 cohol dehydrogenase (AdhA) into the archaeon Pyrococcus furiosus.

23 ss spectrometer to profile the proteome from Pyrococcus furiosus.

24 bus solfataricus, Sulfolobus islandicus, and Pyrococcus furiosus.

25 to those of V-type ATPases, namely that from Pyrococcus furiosus.

26 ssed, using the family-B DNA polymerase from Pyrococcus furiosus.

27 -keto reductase activity native to AdhD from Pyrococcus furiosus.

28 und low-spin FeIII forms of the 1Fe SOR from Pyrococcus furiosus.

29 ophilum (optimal growth at 55 degrees C) and Pyrococcus furiosus (100 degrees C) are homo-dimeric enz

30 e archaeal transcriptional regulator SurR of Pyrococcus furiosus, a hyperthermophilic anaerobe.

31 We have microinjected Box C/D RNAs from Pyrococcus furiosus, a hyperthermophilic archaeon, into

32 l extracts of the hyperthermophilic archaeon Pyrococcus furiosus: a beta-glucosidase, corresponding t

33 e we show that purified Mre11 and Rad50 from Pyrococcus furiosus act cooperatively with HerA and NurA

34 found that the hyperthermophilic archaeaon, Pyrococcus furiosus, actively incorporates DNA fragments

35 This is not the case with Pyrococcus furiosus, an archaeon that grows optimally ne

36 urified from the hyperthermophilic archaeon, Pyrococcus furiosus, an organism that grows optimally at

37 folobales, as well as in other archaea (e.g. Pyrococcus furiosus and Archaeoglobus fulgidus), and the

38 cer integration in vitro using proteins from Pyrococcus furiosus and demonstrate that Cas1 and Cas2 a

39 50 homologues from the thermophilic archaeon Pyrococcus furiosus and demonstrate that the two protein

40 and human clamp loaders, and the two protein Pyrococcus furiosus and Methanobacterium thermoautotroph

41 Divergence of the hyperthermophilic Archaea, Pyrococcus furiosus and Pyrococcus horikoshii, was asses

42 Pyrococcus furiosus and Pyrococcus woesei grow optimally

43 mate dehydrogenase from the hyperthermophile Pyrococcus furiosus and the comparison of this structure

44 bredoxin from the hyperthermophilic archaeon Pyrococcus furiosus and the mesophilic bacterium Clostri

45 gene is highly conserved in Thermotoga spp., Pyrococcus furiosus and Thermococcus kodakarensis, indic

46 te dehydrogenases from the hyperthermophiles Pyrococcus furiosus and Thermococcus litoralis whose opt

47 In the Euryarchaeota species Pyrococcus furiosus and Thermococcus litoralis, phosphog

48 egion between two hyperthermophilic Archaea, Pyrococcus furiosus and Thermococcus litoralis.

49 have applied directed evolution to TrpB from Pyrococcus furiosus and Thermotoga maritima to generate

50 cale applications on Caenorhabditis elegans, Pyrococcus furiosus and three cyanobacterial genomes are

51 se and two proteins of unknown function from Pyrococcus furiosus and Vibrio parahaemolyticus.

52 costs were determined for Escherichia coli, Pyrococcus furiosus, and Chlamydomonas reinhardtii.

53 l element for the hyperthermophilic archaeon Pyrococcus furiosus, and many of its iron-containing enz

54 hia coli, Artemisia tridentata (sage brush), Pyrococcus furiosus, and Methanobacter thermautotrophicu

55 cus litoralis, Thermococcus sp. strain ES-1, Pyrococcus furiosus, and Pyrococcus sp. strain ES-4 cont

56 he metabolite was isolated from the organism Pyrococcus furiosus, and structurally characterized thro

57 cryoEM), we have solved the structure of the Pyrococcus furiosus archaellum filament at 4.2 A resolut

58 Pyrococcus horikoshii (PH1704) and PfpI from Pyrococcus furiosus are members of a class of intracellu

59 ironment, such as hyperthermophilic archaea (Pyrococcus furiosus), are significantly more compact and

60 f a most versatile DNA assembly method using Pyrococcus furiosus Argonaute (PfAgo)-based artificial r

61 We use Pyrococcus furiosus Argonaute to punch files into the PC

62 stal structure of the Argonaute protein from Pyrococcus furiosus at 2.25 angstrom resolution.

63 protein-translocating channel SecYEbeta from Pyrococcus furiosus at 3.1-A resolution suggests a mecha

64 ation cofactor, S-adenosyl-L-methionine from Pyrococcus furiosus, at 2.7 A.

65 ulfolobus solfataricus N-terminal domain and Pyrococcus furiosus ATPase domain.

66 protein from the hyperthermophilic archaeon Pyrococcus furiosus belongs to the Lrp/AsnC family of tr

67 l activator, PF1088, which was identified in Pyrococcus furiosus by a bioinformatic approach.

68 systems, Cmr eliminates plasmid invaders in Pyrococcus furiosus by a mechanism that depends on trans

69 l extracts of the hyperthermophilic archaeon Pyrococcus furiosus by its ability to hydrolyze N-acetyl

70 the proteolytic, hyperthermophilic archaeon Pyrococcus furiosus by multistep chromatography.

71 dase A, but instead resembles neurolysin and Pyrococcus furiosus carboxypeptidase--zinc metallopeptid

72 Here we identified Pyrococcus furiosus Cas6 as a novel endoribonuclease tha

73 CESI-top-down MS analysis of Pyrococcus furiosus cell lysate identified 134 proteins

74 Using reconstituted Pyrococcus furiosus Cmr complexes, we found that each of

75 from cells of the hyperthermophilic archaeon Pyrococcus furiosus contain high hydrogenase activity (9

76 the proteolytic, hyperthermophilic archaeon Pyrococcus furiosus contain high specific activity (11 U

77 annotation of the hyperthermophilic archaeon Pyrococcus furiosus contained 2,065 open reading frames

78 The fermentative hyperthermophile Pyrococcus furiosus contains an NADPH-utilizing, heterot

79 ve shown that the hyperthermophilic archaeon Pyrococcus furiosus contains four distinct cytoplasmic 2

80 (7.5 kDa) of the hyperthermophilic archaeon, Pyrococcus furiosus, contains a single [4Fe-4S]1+,2+ clu

81 In Pyrococcus furiosus, deletion of either histone A or B i

82 The thermostable DNA polymerase derived from Pyrococcus furiosus designated Pfu has the highest fidel

83 The metal-ion-dependent enzyme from Pyrococcus furiosus DSM 3638 showed a relatively high de

84 icus P2), and an anaerobic hyperthermophile (Pyrococcus furiosus DSM 3638).

85 The genome of the hyperthermophile archaeon Pyrococcus furiosus encodes two transcription factor B (

86 DNA, we determined the crystal structures of Pyrococcus furiosus EndoQ bound to DNA substrates contai

87 information has been available only for the Pyrococcus furiosus enzyme (PfMre11), the conserved and

88 se extensions of stabilizing interactions in Pyrococcus furiosus Fd, however, lead to strong destabil

89 The crystal structure of Pyrococcus furiosus FEN-1, active-site metal ions, and m

90 Pyrococcus furiosus ferredoxin (Fd) contains a single [F

91 ication of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.

92 the nature of the residue at position 14 in Pyrococcus furiosus ferredoxin is an important determina

93 e conformationally dynamically heterogeneous Pyrococcus furiosus ferredoxin with an intact disulfide

94 Interestingly, the sFlaF and sFlaG of Pyrococcus furiosus form a globular complex, whereas sFl

95 The hyperthermophilic archaeon Pyrococcus furiosus genome encodes three proteasome comp

96 in the AT-rich Methanococcus jannaschii and Pyrococcus furiosus genomes efficiently detects both kno

97 The anaerobic archaeon Pyrococcus furiosus grows by fermenting carbohydrates pr

98 The hyperthermophilic archaeon Pyrococcus furiosus grows optimally at 100 degrees C by

99 The archaeon Pyrococcus furiosus grows optimally at 100 degrees C by

100 The model archaeon Pyrococcus furiosus grows optimally near 100 degrees C o

101 Pyrococcus furiosus grows optimally near 100 degrees C u

102 ave recently shown that the hyperthermophile Pyrococcus furiosus has an extraordinarily high capacity

103 PF0610, a protein from the hyperthermophile Pyrococcus furiosus, has homologues only in other archae

104 s of SOR from the hyperthermophilic archaeon Pyrococcus furiosus have been determined in the oxidized

105 of the archaeal family-B DNA polymerase from Pyrococcus furiosus have been investigated, illuminating

106 protein from the hyperthermophilic archaeon Pyrococcus furiosus, have been determined in the native

107 r-2 gene from the hyperthermophilic archaeon Pyrococcus furiosus having homology to bacterial and euk

108 hyperthermophiles Pyrococcus horikoshii and Pyrococcus furiosus; however, the form(s) of sulfur that

109 e with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase.

110 The crystal structure of GalK from Pyrococcus furiosus in complex with MgADP and galactose

111 structure-specific 5' flap endonuclease from Pyrococcus furiosus in its complex with DNA.

112 determined the crystal structure of PGI from Pyrococcus furiosus in native form and in complex with t

113 Pyrococcus furiosus is a hyperthermophilic anaerobic arc

114 Pyrococcus furiosus is a hyperthermophilic archaeon that

115 Pyrococcus furiosus is a hyperthermophilic archaeon whic

116 rredoxin from the hyperthermophilic archaeon Pyrococcus furiosus is a monomeric protein (7.5 kDa) tha

117 Pyrococcus furiosus is a strictly anaerobic archaeon (ar

118 The archaeon Pyrococcus furiosus is a strictly anaerobic heterotroph

119 The membrane-bound hydrogenase (Mbh) from Pyrococcus furiosus is an archaeal member of the Complex

120 nase (SHI) of the hyperthermophilic archaeon Pyrococcus furiosus is an NADP(H)-dependent heterotetram

121 Rubredoxin from the hyperthermophile Pyrococcus furiosus is the most thermostable protein cha

122 irst characterized from the hyperthermophile Pyrococcus furiosus, it is unique to the archaeal order

123 Pyrococcus furiosus LrpA forms a homodimer mainly throug

124 tors: Ptr-H10, fusing the effector domain of Pyrococcus furiosus LrpA, and Ptr-H16, fusing the P. fur

125 We determined the crystal structure of the Pyrococcus furiosus MCM N-terminal domain hexamer bound

126 f the type-I (Escherichia coli) and type-II (Pyrococcus furiosus) MetAPs in the presence of the react

127 hermus thermophilus, Archaeoglobus fulgidus, Pyrococcus furiosus, Methanococcus jannaschii, and Metha

128 in DNA double-strand break repair, we report Pyrococcus furiosus Mre11 crystal structures, revealing

129 scattering (SAXS) and crystal structures of Pyrococcus furiosus Mre11 dimers bound to DNA with mutat

130 We found that the analogous mutant of Pyrococcus furiosus Mre11 diminishes both the exonucleas

131 X-ray crystallography of Pyrococcus furiosus Mre11 indicates that an analogous mu

132 Here, using Pyrococcus furiosus Mre11, we question how two Mre11 sep

133 M N-terminal domain of the archaeal organism Pyrococcus furiosus occurs specifically in the hexameric

134 2025) is highly upregulated during growth of Pyrococcus furiosus on elemental sulfur (S(0)).

135 s) that have been annotated in the genome of Pyrococcus furiosus (optimal growth temperature, 100 deg

136 ng enzyme have been previously purified from Pyrococcus furiosus (optimum growth temperature, 100 deg

137 led MATE from the hyperthermophilic archaeon Pyrococcus furiosus Pairs of spin labels monitoring the

138 PH:ferredoxin oxidoreductase II (NfnII) from Pyrococcus furiosus, permitting comparison with work don

139 cluster in the D14C variant ferredoxin from Pyrococcus furiosus (Pf D14C Fd).

140 of the rubredoxins from the hyperthermophile Pyrococcus furiosus (Pf) and the mesophile Clostridium p

141 s of Rds from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) and the mesophilic bacterium Cl

142 se (POR) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) catalyzes the final oxidative s

143 xin (Fd) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) have been characterized by (1)H

144 xin (Fd) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) possesses several unique proper

145 distance to the [Fe(SCys)(4)] center in the Pyrococcus furiosus (Pf) Rd crystal structure compared t

146 Amide exchange rates were measured for Pyrococcus furiosus (Pf) rubredoxin substituted with eit

147 ine monophosphate dehydrogenase (IMPDH) from Pyrococcus furiosus (Pf), a hyperthermophillic archeon.

148 ter of the hyperthermophilic marine archaea, Pyrococcus furiosus (Pf), we have determined the locatio

149 in reduced and oxidized rubredoxin (Rd) from Pyrococcus furiosus (Pf).

150 The Argonaute of the archaeon Pyrococcus furiosus (PfAgo) belongs to a different branc

151 ferritin from the hyperthemophilic bacterium Pyrococcus furiosus (PfFt), have been used as models for

152 nding studies with a mutant of ferritin from Pyrococcus furiosus (PfFtn) in which self-assembly was a

153 crystal structure of a MATE transporter from Pyrococcus furiosus (PfMATE) in the long-sought-after in

154 ent MATE from the hyperthermophilic archaeon Pyrococcus furiosus (PfMATE).

155 cMetAP-I) and the hyperthermophilic archaeon Pyrococcus furiosus (PfMetAP-II) was investigated.

156 coli (EcMetAP-I) and the type II MetAP from Pyrococcus furiosus (PfMetAP-II).

157 eptidase from the hyperthermophilic archaeon Pyrococcus furiosus (PfMetAP-II; EC 3.4.11.18) has been

158 structures of apo- and holorubredoxins from Pyrococcus furiosus (PfRd) and Clostridium pasteurianum

159 rks in rubredoxins from the hyperthermophile Pyrococcus furiosus (PfRd), and its mesophilic analogue

160 luble [NiFe]-hydrogenase: hydrogenase I from Pyrococcus furiosus (PfSHI), which grows optimally near

161 the beta-subunit of tryptophan synthase from Pyrococcus furiosus (PfTrpB).

162 allosteric regulation existing in TrpS from Pyrococcus furiosus (PfTrpS), and how the allosteric con

163 of RPP21 from the hyperthermophilic archaeon Pyrococcus furiosus ( Pfu) using conventional and parama

164 he thermostable family B DNA polymerase from Pyrococcus furiosus (Pfu Pol) contains sensitive determi

165 on the POP of the hyperthermophilic archaeon Pyrococcus furiosus (Pfu) 85 degrees C in both H(2)O and

166 P holoenzyme from the thermophilic archaeon Pyrococcus furiosus (Pfu) and furthered our understandin

167 ities associated with Type III-B immunity in Pyrococcus furiosus (Pfu) are regulated by target RNA fe

168 f-replication (CSR), we evolved a version of Pyrococcus furiosus (Pfu) DNA polymerase that tolerates

169 Pyrococcus furiosus (Pfu) harbors three CRISPR-Cas immun

170 ng strategy to pinpoint the binding sites of Pyrococcus furiosus (Pfu) L7Ae on its cognate RNase P RN

171 ronounced unwinding of primer-templates with Pyrococcus furiosus (Pfu) polymerase-DNA complexes conta

172 We investigated Pyrococcus furiosus (Pfu) RNase P, an archaeal RNP that

173 urthermore, an N-terminal deletion mutant of Pyrococcus furiosus (Pfu) RPP29 that is defective in ass

174 to characterize inter-domain interactions in Pyrococcus furiosus (Pfu) RPR, either alone or with RPPs

175 ectron microscopy (cryo-EM) snapshots of the Pyrococcus furiosus (Pfu) type I-A effector complex in d

176 plification utilized the DNA polymerase from Pyrococcus furiosus (Pfu) which, unlike Taq, does not in

177 ered a thermostable enzyme from the archaeon Pyrococcus furiosus (Pfu), which increases yields of PCR

178 mutants of the family B DNA polymerase from Pyrococcus furiosus (Pfu-Pol), with superb performance i

179 A maltodextrin-binding protein from Pyrococcus furiosus (PfuMBP) has been overproduced in Es

180 maltose binding protein from the thermophile Pyrococcus furiosus (PfuMBP).

181 Pyrococcus furiosus phosphoglucose isomerase (PfPGI) is

182 Pyrococcus furiosus phosphoglucose isomerase (PfPGI), an

183 Transcriptional and enzymatic analyses of Pyrococcus furiosus previously indicated that three prot

184 The hyperthermophilic archeon Pyrococcus furiosus produces an extracellular alpha-amyl

185 d on its amino acid sequence similarity with Pyrococcus furiosus protease I, P. aeruginosa PfpI was o

186 x cleaves an endogenous complementary RNA in Pyrococcus furiosus, providing direct in vivo evidence o

187 In Pyrococcus furiosus, psiRNAs occur in two size forms tha

188 Here we design structure-based mutations in Pyrococcus furiosus Rad50 to alter protein core plastici

189 Based on the recent Pyrococcus furiosus Rad51 structure, we have used homolo

190 ics of rubredoxins from the hyperthermophile Pyrococcus furiosus (RdPf) and the mesophile Clostridium

191 he small iron-sulfur protein rubredoxin from Pyrococcus furiosus (RdPf) at pH 2.

192 of operons in the hyperthermophilic archaeon Pyrococcus furiosus represents an important step to unde

193 obus fulgidus, Methanococcus jannaschii, and Pyrococcus furiosus, respectively.

194 the 2.3 A crystal structure of mre11-3 from Pyrococcus furiosus revealed an active site structure wi

195 re of the protein-protein complex comprising Pyrococcus furiosus RPP21 and RPP29.

196 on Zn(II)-, Ga(III)-, and Ge(IV)-substituted Pyrococcus furiosus rubredoxin demonstrate that the log

197 (Trp) solvation dynamics in water and in the Pyrococcus furiosus rubredoxin protein, including the na

198 static solvent-accessible amide hydrogens of Pyrococcus furiosus rubredoxin range from near the diffu

199 re crystal structure of a hyperthermophilic (Pyrococcus furiosus) rubredoxin at 393 K (120 degrees C)

200 l-atom molecular-dynamics simulations of the Pyrococcus furiosus SecYE, which was determined to be in

201 identification and characterization of SurR, Pyrococcus furiosus sulphur (S(0)) response regulator.

202 perties of the oxidized and reduced forms of Pyrococcus furiosus superoxide reductase (SOR) as a func

203 ction at the mononuclear iron active site of Pyrococcus furiosus superoxide reductase (SOR) through t

204 he site of base exchange; truncated forms of Pyrococcus furiosus TGT retain their specificity for gua

205 ed a gene from the archaeal hyperthermophile Pyrococcus furiosus that reduces O(2)(-).

206 gen exchange results for the rubredoxin from Pyrococcus furiosus, the acidity of these amides was cal

207 etAP from E. coli and the type-II MetAP from Pyrococcus furiosus, the type-I MetAP can be selectively

208 e: the archaea Methanococcus maripaludis and Pyrococcus furiosus; the bacteria Escherichia coli, Stap

209 the N-terminal DNA binding domain of Phr of Pyrococcus furiosus, these results suggest that HSR1 and

210 in from the hyperthermophilic archebacterium Pyrococcus furiosus to understand the unusual temperatur

211 cted 50 representative proteins, mostly from Pyrococcus furiosus, to this pipeline and found that 30

212 (Topt = 37 degrees C) and hyperthermophilic Pyrococcus furiosus (Topt = 95 degrees C) were recorded

213 n initiation complexes have been formed with Pyrococcus furiosus transcription factors (TBP and TFB1)

214 cations with larger quantities (100 ng) of a Pyrococcus furiosus tryptic digest, but with mass-limite

215 The hyperthermophilic archaeon Pyrococcus furiosus uses carbohydrates as a carbon sourc

216 eductase from the hyperthermophilic anaerobe Pyrococcus furiosus uses electrons from reduced nicotina

217 r E17 to Q in the soluble hydrogenase I from Pyrococcus furiosus using site directed mutagenesis.

218 Pyrococcus furiosus utilizes starch and its degradation

219 gene rgy from the hyperthermophilic archaeon Pyrococcus furiosus was cloned and sequenced.

220 n from the hyperthermophilic archaebacterium Pyrococcus furiosus was examined by a hydrogen exchange

221 c activity in the hyperthermophilic archaeon Pyrococcus furiosus was found to be a homomultimer consi

222 cosidase from the hyperthermophilic archaeon Pyrococcus furiosus was recombinantly produced in Escher

223 ock protein (sHSP) from the hyperthermophile Pyrococcus furiosus was specifically induced at the leve

224 lucanase from the hyperthermophilic archaeon Pyrococcus furiosus, was cloned and expressed in Escheri

225 The hyperthermophilic archaeon, Pyrococcus furiosus, was grown on maltose near its optim

226 Herein, the model hyperthermophile, Pyrococcus furiosus, which grows optimally near 100 degr

227 hat confers on a microorganism (the archaeon Pyrococcus furiosus, which grows optimally on carbohydra

228 ned the structures of a PurP orthologue from Pyrococcus furiosus, which is functionally unclassified,

229 amily B DNA polymerases from archaea such as Pyrococcus furiosus, which live at temperatures approxim

230 tryptophan synthase beta-subunit (TrpB) from Pyrococcus furiosus, which mimics the activation afforde

231 l-II family DNA polymerase from the archaeon Pyrococcus furiosus with the aim of improving ddNTP util

232 gineered subunit of tryptophan synthase from Pyrococcus furiosus, yielding (2S,3S)-beta-methyltryptop