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1 though expressed and membrane-localized in a R. capsulatus mutant lacking CcoA, these transporters we
6 ast to wild-type strains, R. sphaeroides and R. capsulatus fnrL mutants do not synthesize the anaerob
8 ment of these natural promoters activated by R. capsulatus RNAP/sigma70 indicated a preference for th
9 Thus, an additional barrier to activation by R. capsulatus NtrC exists, probably a lack of the proper
12 y phenotypes upon overproduction of the CcmF-R. capsulatus CcmH (CcmF-CcmH(Rc)) couple in a growth me
13 osed to act as an apocytochrome c chaperone, R. capsulatus does not have the ability to produce holoc
15 findings therefore demonstrate that, during R. capsulatus growth on minimal medium, the requirement
16 sion in an E. coli plsC(Ts) mutant of either R. capsulatus plsC316 or olsA gene products supported gr
17 r detergent dispersed chromatophore-embedded R. capsulatus bc(1) complex, we demonstrated that while
18 strains were compared with those expressing R. capsulatus CcoA and Rhizobium leguminosarum RibN as b
19 e of -10 promoter mutants did not facilitate R. capsulatus NtrC activation of the nifA1 promoter by t
20 ase (RNAP) that contains the sigma70 factor (R. capsulatus RNAP/sigma70) was purified and characteriz
21 f a peptide designed to serve as a model for R. capsulatus apocytochrome c(2) have also been carried
23 cific transcripts were detected in vitro for R. capsulatus cytochrome c2 (cycA) and fructose-inducibl
25 made with large and small subunit genes from R. capsulatus and R. sphaeroides, also supported the unr
27 obacter sphaeroides, the form I RubisCO from R. capsulatus is a member of the green-like group and cl
29 utants affected in cyt c oxidase activity in R. capsulatus led to the isolation of at least five clas
30 og was identified 127 bp upstream of acxA in R. capsulatus, but this activator lacked key features of
32 oters (nifA1, nifA2, glnB, mopA and anfA) in R. capsulatus which are transcriptionally activated by N
33 an operon essential for cyt c biogenesis, in R. capsulatus, it is located immediately downstream from
34 complementing them revealed that ccoNOQP in R. capsulatus is not flanked by the oxygen response regu
35 Here we identified a CopZ-like chaperone in R. capsulatus, determined its cellular concentration and
37 of the main chain and side chain dynamics in R. capsulatus ferrocytochrome c(2) derived from (2)H NMR
39 Membrane topology of CcdA was established in R. capsulatus using ccdA:phoA and ccdA :lacZ gene fusion
41 e subunits (Bchl and BchN) were expressed in R. capsulatus as S tag fusion proteins that facilitated
43 ggesting that there is only one cbbP gene in R. capsulatus and that this gene is cotranscribed with c
46 s, R. sphaeroides cyt cy can act at least in R. capsulatus as an electron carrier between the cyt bc1
49 olysin, or an endogenous activity present in R. capsulatus, cleaves the hinge region of the Fe-S subu
50 sion carrying the G488A mutation produced in R. capsulatus over 30-fold higher beta-galactosidase act
51 prelude to studies of cbb gene regulation in R. capsulatus, the nucleotide sequence of a 4,537-bp reg
53 equired for glycerophospholipid synthesis in R. capsulatus, while olsA acts as an alternative AGPAT t
55 wn to be Ps(+) Nadi(+), establishing that in R. capsulatus the inactivation of dsbA suppresses the c-
60 ted in the hinge region (positions 43-49) of R. capsulatus Fe-S subunit was not essential per se for
61 mediator ADP had no effect on the ability of R. capsulatus LPS to stimulate NO production but signifi
62 oteins either greatly reduced the ability of R. capsulatus to support growth or had little effect, re
65 on of the heme-Cu-containing subunit CcoN of R. capsulatus cbb(3)-Cox proceeds independently of that
66 epitope-tagged and functional derivative of R. capsulatus cyt c(y) was purified from intracytoplasmi
67 e that some OlsA enzymes, like the enzyme of R. capsulatus, are bifunctional and involved in both mem
68 s shown here that the ccl1 and ccl2 genes of R. capsulatus are required for the synthesis of all c-ty
70 upports efficiently photosynthetic growth of R. capsulatus in the absence of cyt c2 because it can me
71 was able to support photosynthetic growth of R. capsulatus in the absence of the cyt c(y) and c2.
75 he DsbA-null and DsbB-null single mutants of R. capsulatus are Ps(+) and produce c-type cytochromes,
82 wever, R. sphaeroides cyt cy, unlike that of R. capsulatus, is unable to function as an efficient ele
84 s NtrC exists, probably a lack of the proper R. capsulatus NtrC-E. coli RNA polymerase (protein-prote
86 mation and function showed that the purified R. capsulatus sigma N protein is distinct in activity co
87 uted in vitro to form an active, recombinant R. capsulatus RNA polymerase with properties mimicking t
89 the R. capsulatus bchM mutant via the strong R. capsulatus puc promoter was shown to support nearly w
90 ents confirm earlier results indicating that R. capsulatus ferrocytochrome c(2) exhibits minor rotati
91 molecular genetic studies, we inferred that R. capsulatus CcmF, CcmH, and CcmI interact with each ot
95 NtrC enhancer-binding protein activates the R. capsulatus housekeeping RNA polymerase but not the Es
96 but contact of certain promoter bases by the R. capsulatus sigma N protein and its response to core R
100 expression of the Synechocystis gene in the R. capsulatus bchM mutant via the strong R. capsulatus p
102 rol of the CBB pathway and regulation of the R. capsulatus cbb genes were studied by using a combinat
103 taI, resulted in decreased production of the R. capsulatus gene transfer agent, and gene transfer age
104 . autotrophicus and sequence analysis of the R. capsulatus genome and were found to be clustered in s
105 sm was used to evaluate the structure of the R. capsulatus protein and revealed differences in the te
106 protein that activates transcription of the R. capsulatus sigma 70 RNA polymerase, but does not acti
107 To test the -35 recognition pattern, the R. capsulatus nifA1 promoter, which exhibits only three
108 facile genetic manipulation, we purified the R. capsulatus form I enzyme and determined its basic kin
113 A kinetic isotope study of the wild-type R. capsulatus enzyme indicates that, as previously deter
116 nisms that govern the diverse means by which R. capsulatus maintains redox poise during photoheterotr