ライフサイエンスコーパス: RNA cleavage

1 cellular RISC machinery for inducing target RNA cleavage.

2 ganic phosphate as a nucleophile to catalyze RNA cleavage.

3 s, we defined the molecular requirements for RNA cleavage.

4 ecreted and endocytosed, but fails to induce RNA cleavage.

5 tic tetrad, thereby activating Argonaute for RNA cleavage.

6 ven mRNAs that are resistant to nsp1-induced RNA cleavage.

7 nucleobase general acid strategies to effect RNA cleavage.

8 plays an essential role in the catalysis of RNA cleavage.

9 domains come together to drive Las1-mediated RNA cleavage.

10 ell-shaped pH versus k(cat)/K(M) profile for RNA cleavage.

11 ral elements within BLM that control DNA and RNA cleavage.

12 sses such as transcription, translation, and RNA cleavage.

13 thermophilus, inhibits rather than activates RNA cleavage.

14 RISC assembly, or multiple rounds of target-RNA cleavage.

15 lex (RISC), which catalyzes target messenger RNA cleavage.

16 also associate with Ago2 for guiding target RNA cleavage.

17 ch catalyzes site specific, Pb(2+)-dependent RNA cleavage.

18 common small DNA motif capable of catalyzing RNA cleavage.

19 by a translational mechanism rather than by RNA cleavage.

20 e protein-siRNA complex that mediates target RNA cleavage.

21 entary sequences without inducing detectable RNA cleavage.

22 egies to achieve overall rate enhancement of RNA cleavage.

23 ysis of the wild-type ribozyme to facilitate RNA cleavage.

24 their ability to enhance enzyme catalysis of RNA cleavage.

25 hat are necessary for efficient catalysis of RNA cleavage.

26 which serves as an acid during catalysis of RNA cleavage.

27 mains generating a potential active site for RNA cleavage.

28 nation and, in some cases, cotranscriptional RNA cleavage.

29 tide 320) and that is essential for accurate RNA cleavage.

30 role, to act as an acid during catalysis of RNA cleavage.

31 termediate in factor-induced endonucleolytic RNA cleavage.

32 ar RNase H enzyme for hybridization-directed RNA cleavage.

33 RNA; however, HDAgs are not required for HDV RNA cleavage.

34 y of HDAg is required for the enhancement of RNA cleavage.

35 -through and carry out SII-activated nascent RNA cleavage.

36 e crRNAs for activation of Cas13a collateral RNA cleavage.

37 mination mechanism does not require upstream RNA cleavage.

38 loop (TL), for both nucleotide addition and RNA cleavage.

39 kely catalyze the proton-relay mechanism for RNA cleavage.

40 oximity without involving Argonaute-mediated RNA cleavage.

41 the cleaving site was required for efficient RNA cleavage.

42 mplicated in both sRNA maturation and target RNA cleavage.

43 ich enables rapid recovery from any depth by RNA cleavage.

44 kinetic competition between 1D diffusion and RNA cleavage.

45 HEPN nuclease motif (RphiXXXH) important for RNA cleavage.

46 Very few microRNAs are known to guide RNA cleavage.

47 ed RNAs is probably created by site-specific RNA cleavage.

48 eting, in vitro data have shown crRNA-guided RNA cleavage.

49 allowing piRNAs to spread beyond the site of RNA cleavage.

50 ributes to human diseases via stress-induced RNA cleavage.

51 rted to be the most efficient lanthanide for RNA cleavage.

52 equence-specific cis- and non-specific trans-RNA cleavage(8).

53 nificantly inhibited human RNase H-catalyzed RNA cleavage (80-90% inhibition) and that a similar inhi

54 entical to that emptied during SII-activated RNA cleavage, a process required for the resumption of e

55 and the distance between the pocket and the RNA cleavage active site in the RNaseIII domain correspo

56 ize the mechanisms of the type I and type II RNA cleavage activities harbored by the Flp (pronounced

57 entiates the weak, intrinsic, piRNA-directed RNA cleavage activities of PIWI proteins, transforming t

58 cific DNA recombinase Flp shows two types of RNA cleavage activities on hybrid DNA-RNA substrates.

59 her and how Cas13 uses its cis- and/or trans-RNA cleavage activities to defend against double-strande

60 trand breakage and joining, and two types of RNA cleavage activities.

61 utation of lysine to glutamic acid abolished RNA cleavage activity in the absence of a divalent metal

62 In addition, Fap7 inhibits the RNA cleavage activity of Nob1, the endonuclease responsi

63 levels and activity correlated well with the RNA cleavage activity of the DNAzyme.

64 We present the evidence for the specific RNA cleavage activity of the engineered catalytic subuni

65 tively catalytic residues, has no detectable RNA cleavage activity on its own but is active upon mixi

66 t neither residue contributes to the type II RNA cleavage activity or to the strand-joining reaction

67 revailing tool, CRISPR-Cas13, has collateral RNA cleavage activity that causes undesirable cytotoxici

68 ently linked catalytic homodimer that had no RNA cleavage activity upon mixing with the structural su

69 Ribozyme-mediated RNA cleavage activity was detected in cell extracts.

70 cleosome, suggesting that upon inhibition of RNA cleavage activity, RNAPII arrest prevalently occurs

71 ole in splicing involves RNA binding but not RNA cleavage activity.

72 Argonaute2, being responsible for messenger RNA cleavage activity.

73 ermination, and does so independently of its RNA cleavage activity.

74 and what factors contribute to regulation of RNA cleavage activity.

75 asmids and DNA viruses requires DNA, but not RNA, cleavage activity.

76 Different from its known cis/trans RNA-cleavage activity, Cas13 can also cleave host RNA vi

77 to achieve both enhanced DNA and diminished RNA-cleavage activity.

78 ely 100-fold increase in the ratio of DNA to RNA-cleavage activity.

79 t, with the products then being selected for RNA-cleavage activity.

80 each its catalytic potential as a biomimetic RNA cleavage agent.

81 ating enhanced biomimetic, sequence-specific RNA cleavage agents.

82 Rescue from backtracking by RNA cleavage also promotes escape from 5' pause sites, p

83 The results of RNA cleavage analyses suggest that KHSO5- or magnesium m

84 protein), presumably by promoting such rapid RNA cleavage and 5' exonucleolytic degradation that PPR1

85 ns C-terminal RNase III domains that mediate RNA cleavage and an N-terminal helicase motif, whose fun

86 rn2 attacks the 5'PO4-end exposed by nascent RNA cleavage and chases down the RNA polymerase.

87 ibonuclease Csx1, and showed that subsequent RNA cleavage and dissociation acts as an 'off-switch' fo

88 2a, and Cas13a that mediated multiplexed DNA/RNA cleavage and gene regulation in cell-free systems, b

89 RISPR Cas effector that confers programmable RNA cleavage and has potential applications in RNA inter

90 mes have the potential to perform successive RNA cleavage and joining reactions, resulting in their m

91 The catalysis of site-specific RNA cleavage and ligation by the hairpin ribozyme requir

92 ellite (VS) ribozyme catalyzes site-specific RNA cleavage and ligation reactions.

93 d satellite ribozyme catalyses site-specific RNA cleavage and ligation, and serves as an important mo

94 Together, Las1L-Nol9 catalyzes RNA cleavage and phosphorylation to mark the ITS2 for de

95 function of CPSF in mediating PAS-dependent RNA cleavage and polyadenylation.

96 snapshots of Craspase to explain its target RNA cleavage and protease activation mechanisms.

97 e, which shows the conformation required for RNA cleavage and proximity of the 2'-hydroxyl group to t

98 lt of loss of the 3'-UTR due to ASO-mediated RNA cleavage and retention of the last intron.

99 ns on the surface of ER membranes to promote RNA cleavage and ribonucleoprotein (RNP) removal.

100 Finally, we connect RNA cleavage and splicing with either premature or full-

101 ant-negative TFIIS (TFIIS(DN)) that inhibits RNA cleavage and stabilizes backtracked complexes.

102 rent segments of the Rne protein to catalyze RNA cleavage and to bind RNA, we found that the N-termin

103 When TFIIS is present, RNA cleavage and transcriptional restart pathways are su

104 They can catalyze site-specific RNA cleavage, and as a result, they have relevance in ge

105 d Cmr5), each Cmr4 subunit mediates a target RNA cleavage, and Cmr1 and Cmr6 mediate an essential int

106 s and/or targets, HrAgo1 mediates RNA-guided RNA cleavage, and facilitates gene silencing when expres

107 ly 28 s(-1)) was comparable with that of net RNA cleavage ( approximately 27 nucleotides(s)).

108 t the functions of the TL and Gre factors in RNA cleavage are conserved in various species, with impo

109 demonstrate that miRNA processing and target-RNA cleavage are coupled.

110 At the pause sites, the burst amplitudes of RNA cleavage are larger than the corresponding reaction

111 consistent with a two-metal ion mechanism of RNA cleavage as previously suggested for a number of pol

112 CV John Cunningham 1/AAG mutant and in vitro RNA cleavage assay demonstrated that MCPIP1 could direct

113 In vitro RNA cleavage assay demonstrated that the ribozyme select

114 Here, we used an RNA cleavage assay to show that the PEG and Ficoll crowd

115 small-angle x-ray scattering and biochemical RNA cleavage assays reveal globally similar structural a

116 RNA cleavage assays showed that S. thermophile Dicer-1 (

117 a- and XBP1-depleted cells, validation using RNA cleavage assays, and 5' RACE identified the prooncog

118 enzymatic activity of DICER1 using in vitro RNA cleavage assays.

119 appeared functionally equivalent in in vitro RNA cleavage assays.

120 ch activity was detected and increased total RNA cleavage at high Mg(2+) concentrations sufficient to

121 ace of Fe(2+) in supporting the catalysis of RNA cleavage at neutral pH, but not at lower pH.

122 for efficient termination, cotranscriptional RNA cleavage at the poly(A) site is not.

123 I, and that loss of EF-RNA interactions upon RNA cleavage at the polyadenylation site triggers disass

124 catalytic core that preferentially catalyzes RNA cleavage at UN dinucleotide junctions (k(obs) = 0.9

125 P23 are essential for early pre-ribosomal (r)RNA cleavages at sites A0, A1/1 and A2/2a in yeast and h

126 se subunits and/or their distinct domains on RNA cleavage, backtracking, and transcription fidelity i

127 Plant microRNAs (miRNAs) typically mediate RNA cleavage, but examples of miRNA-mediated translation

128 t) lacks the stimulatory activity to promote RNA cleavage, but instead efficiently inhibits unstimula

129 ORF17 protein induced RNA cleavage, but to a substantially lesser extent than

130 ozymes have been identified for catalysis of RNA cleavage by 2'-hydroxyl transesterification, forming

131 tify the smallest RNA that can direct target RNA cleavage by 3' tRNase.

132 small interfering RNAs that guide transgene RNA cleavage by AGO1.

133 eletions of the TL strongly impair intrinsic RNA cleavage by all three RNAPs and eliminate the inters

134 a protein structural change that accelerates RNA cleavage by another subunit.

135 Here, we report that RNA cleavage by Argonaute3 initiates production of most

136 Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable R

137 se function of HIV-1 RT and then to abrogate RNA cleavage by HIV-1 RNaseH.

138 investigated the ability of DNA to catalyze RNA cleavage by hydrolysis rather than transesterificati

139 We demonstrate that NusG slows RNA cleavage by inhibiting backtracking.

140 The value of k(cat)/K(M) for catalysis of RNA cleavage by ribonuclease (RNase) A can exceed 10(9)

141 s12 and His119 are critical for catalysis of RNA cleavage by ribonuclease A (RNase A).

142 molecules: the light-regulation of catalytic RNA cleavage by RISC and the light-regulation of seed re

143 RNA cleavage by RNA polymerase (RNAP) is the central ste

144 a universal bacterial factor that stimulates RNA cleavage by RNA polymerase (RNAP), the functions of

145 This incongruity indicates that catalysis of RNA cleavage by RNase A is limited by the rate of substr

146 RNA cleavage by RNase H requires the presence of divalen

147 eopure ASOs, 3'-SpSpRp, that promotes target RNA cleavage by RNase H1 in vitro and provides a more du

148 ensing, which immediately triggered cellular RNA cleavage by RNase L and arrested host protein synthe

149 slation initiation factor eIF2 and stimulate RNA cleavage by RNase L.

150 sm for Pol I termination: co-transcriptional RNA cleavage by Rnt1 provides an entry site for the 5'-3

151 RNA cleavage by some endoribonucleases and self-cleaving

152 group I and group II intron RNAs, as well as RNA cleavage by the aI5gamma-derived D135 ribozyme.

153 Premature termination requires RNA cleavage by the endonuclease subunit of Integrator,

154 y and the molecular mechanism that underlies RNA cleavage by the RNase E/G family.

155 of thymidine 5'-p-nitrophenyl phosphate and RNA cleavage by the RNase P ribozyme.

156 GreA factors activate RNA cleavage by wild-type RNAPs to similar levels.

157 MS) to monitor the kinetics and products of RNA cleavage, by use of a program designed to mass-match

158 RNA cleavage can also be stimulated by universal Gre fac

159 enomena of termination and cotranscriptional RNA cleavage can be uncoupled, and the efficiency of bot

160 ts (5' fragments) produced by miRNA-mediated RNA cleavage can be uridylated in plants and animals.

161 Both piRNA production and function require RNA cleavage catalysed by PIWI proteins.

162 in the reactions of nucleotide addition and RNA cleavage catalyzed by RNAP.

163 /mol reduction in activation free energy for RNA cleavage catalyzed by the HDV ribozyme.

164 Reciprocal cycles of piRNA-directed RNA cleavage--catalyzed by the PIWI proteins Aubergine (

165 Despite the conserved RNA cleavage chemistry and a similar enzyme assembly, cu

166 or, and Symplekin, a protein associated with RNA cleavage complexes but also involved in cleavage-ind

167 Measurement of the steady state rates of RNA cleavage confirms that all substrates dissociate slo

168 ide was released from the complex by nascent RNA cleavage, demonstrating that this interaction takes

169 We combine a general RNA cleavage domain with a series of Pumilio/fem-3-bindi

170 op (TL), to show that the TL is required for RNA cleavage during proofreading of misincorporation eve

171 ed with stress granules from RNAse L-induced RNA cleavage during viral infection.

172 The RNA cleavage efficiency was found in all cases to be spe

173 tary sequence beyond guide-target duplex for RNA cleavage, establishing the functional significance o

174 in vivo reveals a second co-transcriptional RNA cleavage event at T1 which provides Pol I with an al

175 RNA-mediated RNA cleavage events are being increasingly exploited to

176 Thus, RNA cleavage events catalyzed by RNase L are required fo

177 doplasmic reticulum unfolded protein load to RNA cleavage events that culminate in the sequence-speci

178 les of the poly(A) signal, cotranscriptional RNA cleavage events, and 5'-3' exonucleolytic RNA degrad

179 subunit Rpb9p, and the pol II elongation and RNA cleavage factor, TFIIS, respectively.

180 pproach to study the requirements of hairpin RNA cleavage for sugar and base moieties in residues of

181 in Saccharomyces cerevisiae does not rely on RNA cleavage for termination but instead terminates via

182 aG), which serves as the attacking group for RNA cleavage, forms a coplanar base triple with the G264

183 oth the full-length, and all of the possible RNA cleavage fragments that resulted from the combinatio

184 We used an nsp1 mutant, nsp1-CD, lacking the RNA cleavage function, to delineate the mechanism of nsp

185 t the role of metal ions in the mechanism of RNA cleavage has not been resolved.

186 nucleic acid (LNA) designed to induce target RNA cleavage have been shown to have enhanced potency al

187 ymes capable of catalyzing sequence-specific RNA cleavage have found broad applications in biotechnol

188 templates that are resistant to nsp1-induced RNA cleavage, implying the validity of using nsp1-CD to

189 aPKR, for their ability to effect target PKR RNA cleavage in a cell-free and in an intact cell assay,

190 d the third Cas7-like domains mediate target RNA cleavage in a metal-dependent manner.

191 de a cynosure for understanding catalysis of RNA cleavage in a system of high medicinal relevance.

192 o significantly affect the rate of intrinsic RNA cleavage in a TL-dependent manner.

193 experimental measurement on the spontaneous RNA cleavage in an in vitro evolved ATP aptamer motives

194 promoter complexes whereas GreB facilitates RNA cleavage in arrested elongation complexes (ECs).

195 e angiogenin acts unidirectionally to induce RNA cleavage in astroglia, while the ALS-associated K40I

196 Rho- or Mfd-mediated RNA release or nascent RNA cleavage in backtracked complexes, the regulatory ta

197 her the TL nor GreA can efficiently activate RNA cleavage in certain types of backtracked transcripti

198 s like RNase H and is responsible for target RNA cleavage in RNA interference.

199 d deglycoBLM analogues were shown to mediate RNA cleavage in the absence of added Fe(2+).

200 In vitro, NoV B2 inhibits Dicer-mediated RNA cleavage in the absence of any other host factors an

201 essures, such as the requisite of catalyzing RNA cleavage in the absence of Mg(2+) or Mn(2+).

202 ex DNA, sequence-selective DNA cleavage, and RNA cleavage in the presence and absence of a metal ion

203 self-cleaving RNA that can be engineered for RNA cleavage in trans and has potential as a therapeutic

204 The requirement for two modes of RNA cleavage in viral replication and the unexpected req

205 ed AGOs can mediate a single round of target RNA cleavage in vitro, accessory factors are required fo

206 mechanism for the differential regulation of RNA cleavages in E. coli.

207 s with B-Catenin to transactivate STC1 in an RNA cleavage-independent manner, contributing to breast

208 at the RNA binding property of VapC-mt4, not RNA cleavage, initiates toxicity.

209 d a 6-base recognition sequence, UACAUA, for RNA cleavage instead of the 5-base sequence, UACAU, for

210 Beyond RNA cleavage, Integrator regulates transcription initiat

211 We also demonstrate that CRISPR-based RNA cleavage is effective for regulation in bacteria, ar

212 Substrate RNA cleavage is gRNA directed and occurs 3' to the uridy

213 It is not clear whether RNA cleavage is sufficient to trigger nuclear RNA degrad

214 tion as a possibly important intermediate in RNA cleavage, its structure has been captured in various

215 a multi-layered defence that includes target RNA cleavage, licencing of an HD DNA nuclease domain and

216 riety of chemical transformations, including RNA cleavage, ligation, and synthesis, as well as alkyla

217 9, RPP30 and L7Ae-EDTA-Fe) revealed specific RNA cleavages, localizing the binding sites of L7Ae to t

218 ity to down-regulate gene expression through RNA cleavage makes the hammerhead ribozyme a candidate f

219 We suggest that SymE promotion of RNA cleavage may be important for the recycling of RNAs

220 w that a lincRNA-specific co-transcriptional RNA cleavage mechanism acts to induce premature terminat

221 Biochemical assays support an autonomous RNA cleavage mechanism independent of RNase H1 engagemen

222 We describe a new RNA cleavage motif, found in the hammerhead ribozyme.

223 cleotide incorporation, sequential rounds of RNA cleavage occurred each time after approximately 6 nu

224 In accord with earlier studies with model RNAs, cleavage occurs only in the presence of manganese

225 ion elongation factor S-II (TFIIS)-dependent RNA cleavage, or as drastic as premature transcription t

226 Furthermore, our analysis showed that the RNA cleavage pathway is also present in human cells but

227 HEPN nucleases participate in diverse RNA cleavage pathways and share a short HEPN nuclease mo

228 PN nucleases participate in stress-activated RNA cleavage pathways; Las1 plays a fundamental role in

229 ons with RNase H1 as evidenced by changes in RNA cleavage patterns, these were insufficient to improv

230 proposed to be non-specific in their in vivo RNA cleavage preference.

231 y cap binding to the PB2 subunit, from which RNA cleavage preferentially occurs at the 12th nt downst

232 into how DNA molecules are able to solve the RNA-cleavage problem, and establishes functional relatio

233 rived from the HIV-1 genome, six predominant RNA cleavage products are found during DNA synthesis cat

234 rast, both intrinsic and TFIIS-induced small RNA cleavage products are very similar when produced fro

235 line, Hey1b, resulted in specific ribosomal RNA cleavage products coinciding with JNK activation.

236 The RNA cleavage products consisted of a 3' phosphate and 5'

237 -linked oligoadenylate (2-5A) produces small RNA cleavage products from self-RNA that initiate IFN pr

238 RNA cleavage products generated by RNase L enhance IL-1b

239 fter WNV infection and the patterns of viral RNA cleavage products generated were similar in both typ

240 Enzymatic analysis of RNA cleavage products has suggested that human immunodef

241 We show that RNA cleavage products of RNase L activity induce the for

242 Both cellular and viral RNA cleavage products of RNase L bind pattern recognitio

243 mass-match observed MS peaks with predicted RNA cleavage products.

244 he first of their kind in terms of their DNA-RNA cleavage properties, and they may have important bio

245 However, the major function of His119 in RNA cleavage, protonation of the 5'-O leaving group, is

246 apsid-specific antiserum eliminated specific RNA cleavage provides further evidence that the virus ca

247 6P is used by the ribozyme as a coenzyme for RNA cleavage, rather than an allosteric effector.

248 The RNA cleavage reaction catalyzed by the hairpin ribozyme

249 The hairpin ribozyme catalyzes a reversible RNA cleavage reaction that participates in processing in

250 hepatitis delta virus ribozyme catalyzes an RNA cleavage reaction using a catalytic nucleobase and a

251 site captured the pre-catalytic state of the RNA cleavage reaction, illustrating the unexpected Pb(2+

252 n ribozyme, catalyzes a multistep reversible RNA cleavage reaction, which comprises two structural tr

253 lge backbones that are poised for an in-line RNA cleavage reaction.

254 combination active site, exhibits the type I RNA cleavage reaction.

255 uses histidine as an active component for an RNA cleavage reaction.

256 emplate arrest sites by activating a nascent RNA cleavage reaction.

257 ulate the free energy surface underlying the RNA-cleavage reaction and characterize its mechanism.

258 oordination emerge as central factors in the RNA-cleavage reaction.

259 activity was detected during activation and RNA cleavage reactions with human RNase L.

260 ns as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression.

261 llance and how it carries out crRNA-mediated RNA cleavage remain unclear.

262 e contributions of the TL and Gre factors to RNA cleavage reportedly vary between RNAPs from differen

263 little or no influence over single-stranded RNA cleavage, RI evasion or cytotoxicity.

264 quired for crRNA-meditated sequence-specific RNA cleavage, RNA target-dependent non-specific DNA clea

265 In addition, RNA cleavage site choice by the full polymerase is also

266 However, the two enzymes showed identical RNA cleavage site preferences with an mRNA as substrate.

267 used to define transcription initiation and RNA cleavage sites associated with all polyadenylated vi

268 These major RNA cleavage sites correlate well with the pause sites s

269 ructural explanation of accessibility of the RNA cleavage sites to the peptide with adoption of an 'i

270 related to vertebrate and yeast primary pre-RNA cleavage sites, respectively.

271 tic activity and deep mutational scanning of RNA cleavage sites-that reveal distinct rules governing

272 The RNA cleavage ("Slicer") activity of Argonaute has been i

273 AGO1-catalyzed RNA cleavage (slicing) represses miRNA targets, but curr

274 RNA cleavage specificity for these VapCs mapped to motif

275 der oligomer species that possesses distinct RNA cleavage specificity from that of previously charact

276 We show that this RNA cleavage step is essential for assembly of the Csy p

277 ic activities, including DNA polymerization, RNA cleavage, strand transfer, and strand displacement s

278 of the FL and TL mutations on GreA-assisted RNA cleavage suggest that the FL-dependent TL transition

279 the resulting enzymes are more efficient at RNA cleavage than most Mg(2+)-dependent nucleic acid enz

280 RNase A is a far more efficient catalyst of RNA cleavage than ONC but is not cytotoxic.

281 omplete complementarity, resulting in target RNA cleavage that is a critical step for target silencin

282 y-detailed and semi-quantitative analysis of RNA cleavage that should be widely applicable.

283 Analysis of donor RNA cleavage, the acceptor invasion site and R homology

284 hough it is dispensable for 5'-end-dependent RNA cleavage, the carboxy-terminal half of RNase E signi

285 to these sites, where they induce efficient RNA cleavage, thereby revealing compact RNA 3-D modules.

286 from shallow backtracks by 1D diffusion, use RNA cleavage to recover from intermediary depths, and ar

287 in RNA interference (RNAi) pathways to guide RNA cleavage, translational repression, or methylation o

288 ities of cross-linked ribozymes to carry out RNA cleavage under single turnover conditions were compa

289 ze of Cas13 effectors and their non-specific RNA cleavage upon target activation limit the adeno-asso

290 e describe the molecular mechanism of target RNA cleavage using affinity-purified minimal RISC from h

291 To confirm that ribozyme-catalyzed RNA cleavage was actually needed for inhibition, we perf

292 The specific role of Ago2 in guiding target RNA cleavage was confirmed independently by siRNA-based

293 RNA cleavage was observed only in the absence of zinc.

294 by its ability to induce an endonucleolytic RNA cleavage, was separable from its translation inhibit

295 hat might be responsible for double-stranded RNA cleavage, we analysed csp41a and csp41b knock-out mu

296 Using nucleotide modifications that inhibit RNA cleavage, we show that R- but not L-sshRNAs require

297 While Mg(2+) enables eEndoV to catalyze RNA cleavage, we show that similar levels of Ca(2+) inst

298 Sites of RNA cleavage were mapped by sequencing reactions.

299 rovements in efficiency at sequence-specific RNA cleavage when compared with analogous o-phenanthroli

300 ontaining complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency o