ライフサイエンスコーパス: RNA primer

1 upon attachment of a deoxynucleotide to the RNA primer).

2 primer synthesis but was not copied into the RNA primer.

3 ng a short 3'-ribonucleotide tract to an all-RNA primer.

4 t the (-)-strand DNA template and (+)-strand RNA primer.

5 ive replication of mtDNA by generation of an RNA primer.

6 as almost inactive on a non-polypurine tract RNA primer.

7 s known to interact with the single-stranded RNA primer.

8 ion of primase and assembly of beta onto the RNA primer.

9 mentary DNA "bubble" containing a hybridized RNA primer.

10 annealed just adjacent to the 5'-end of the RNA primer.

11 g the sugar-phosphate backbone of the DNA or RNA primer.

12 iation of Okazaki fragment synthesis from an RNA primer.

13 azaki fragment to a clamp assembled on a new RNA primer.

14 replication fork and synthesizes the Okazaki RNA primers.

15 pposite to that predicted to bind elongating RNA primers.

16 winding double-stranded DNA and synthesizing RNA primers.

17 , the two together catalyze the synthesis of RNA primers.

18 hat RT binds preferentially to the 5' end of RNA primers.

19 ilization or suppress extension from non-PPT RNA primers.

20 is using Helicobacter-specific 16S ribosomal RNA primers.

21 of the enzyme activities that produce capped RNA primers.

22 ed in protein-nucleic acid interactions with RNA primers.

23 templates having upstream DNA and downstream RNA primers.

24 The RNA primer 3' end is positioned 5 angstrom away from the

25 f the greater distance between the attacking RNA primer 3'-hydroxyl and the phosphate of the incoming

26 of a primase heterodimer that synthesizes an RNA primer, a DNA polymerase subunit that extends the pr

27 ts of a primase heterodimer that synthesizes RNA primers, a DNA polymerase that extends them, and a f

28 consequences of 8-Cl-Ado incorporation into RNA primers, a synthetic RNA primer containing a 3'-term

29 plex DNA at a replication fork, synthesis of RNA primers along the lagging strand and hand-off to Dna

30 through a promoter by adding a complementary RNA primer and core Escherichia coli RNA polymerase in t

31 died the role of complementarity between the RNA primer and the acceptor site at DR2 in HBV.

32 ased on sequence complementarity between the RNA primer and the R2 template.

33 ere generated by a failure to remove the PPT RNA primer and/or by mispriming at sites upstream of the

34 ependent enzymes, a primase that synthesizes RNA primers and a DNA polymerase that elongates them.

35 ein in controlling T4 RNase H degradation of RNA primers and adjacent DNA during each lagging strand

36 fined origins, replication bidirectionality, RNA primers and leading and lagging strand synthesis.

37 ar assembly that enhances the utilization of RNA primers and may functionally couple leading and lagg

38 A replication system, T4 RNase H removes the RNA primers and some adjacent DNA before the lagging str

39 e role for the antipodal sites in removal of RNA primers and the repair of gaps in newly replicated m

40 o their RNA complements from a surface-bound RNA primer, and the DNA templates are enzymatically dest

41 types of RNA-DNA hybrids, including R-loops, RNA primers, and ribonucleotide misincorporations, that

42 Downstream DNA primers, RNA primers, and small 5'-flaps were efficiently matured

43 sively, removing adjacent DNA as well as the RNA primers, and that the difference in the relative rat

44 leotides, unwinding of DNA, the synthesis of RNA primers, and the assembly of proteins on DNA.

45 hybrid consisting of a 15- or 20-nucleotide RNA primer annealed to a 35-nucleotide DNA template is c

46 Using substrates having RNA primers annealed to circular DNA templates, we showe

47 of the contacts observed previously with an RNA primer are preserved with a DNA primer--with the sam

48 how, using the T7 replication proteins, that RNA primers are made 'on the fly' during ongoing DNA syn

49 Unlike Pol II, Pols IV and V require an RNA primer, are insensitive to alpha-amanitin, and diffe

50 e hairpin structures II and III of the ColE1 RNA primer as determinants of plasmid compatibility.

51 synthesized and hybridized to PPT-containing RNA primers as a means of locally removing hydrogen bond

52 ocessivity factor by partially enclosing the RNA primer at the heterodimer interface.

53 Primases synthesize RNA primers at a rate that is orders of magnitude lower

54 SV40) DNA replication in vitro, synthesis of RNA primers at the origin of replication requires only t

55 by RNase H likely eliminates many potential RNA primers, based on thermostability predictions it app

56 adding a stretch of deoxynucleotides to the RNA primer before handoff to PolC.

57 requires complete removal of the initiating RNA primer before ligation occurs.

58 merase, while the error-prone DnaEBs extends RNA primers before hand-off to PolC at the lagging stran

59 rone tRNA(3)(Lys) placement onto the genomic RNA primer binding site; however, the timing and possibl

60 A model for RNA primer binding that involves all three VP55 domains

61 We show that the capped RNA primer binds at the cap-binding site and induces a c

62 oside triphosphates, the ribozyme extends an RNA primer by successive addition of up to six mononucle

63 fragment is initiated by the synthesis of an RNA primer by the gene 4 primase at specific recognition

64 suggests that subsequent degradation of the RNA primer by the RNase H domain was required for strand

65 information of an RNA template to extend an RNA primer by the successive addition of up to 14 nucleo

66 Polyadenylation of this modified RNA primer by yPAP in the presence of ATP was blocked co

67 mase is also required for the utilization of RNA primers by T7 DNA polymerase.

68 Finally, our analysis indicates the entire RNA primer can contribute to primer translocation and is

69 incorporation into RNA primers, a synthetic RNA primer containing a 3'-terminal 8-Cl-AMP residue was

70 and the isolated polymerase domain extended RNA primers containing the PPT sequence irrespective of

71 g orientations on duplexes containing DNA or RNA primers, directing its DNA synthesis or RNA hydrolys

72 uclease essential for the degradation of the RNA primer-DNA junctions at the 5' ends of immature Okaz

73 st Pol alpha in unliganded form, bound to an RNA primer/DNA template and extending an RNA primer with

74 synthesis, but extension of the synthesized RNA primer does not occur.

75 art of DNA polymerase alpha that synthesizes RNA primers during DNA replication.

76 lular survival requires efficient removal of RNA primers during lagging strand DNA synthesis.

77 DNA polymerase I (pol I) processes RNA primers during lagging-strand synthesis and fills sm

78 amount of evidence indicates the presence of RNA primers during mtDNA replication, this result might

79 Transcription is initiated with capped RNA primers excised from cellular pre-mRNAs by the intri

80 x and an abortive complex containing a short RNA primer extending to +3 were characterized.

81 aged ribonucleotide 1,N (6)-erA but has poor RNA primer extension activities.

82 putative promoter element was identified by RNA primer extension analysis upstream of the ABCD opero

83 Using RNA primer extension assays, we determined that the mdm-

84 A working model of nonenzymatic RNA primer extension could illuminate how prebiotic chem

85 Non-enzymatic template-directed RNA primer extension is a model of the copying step in t

86 inetic analysis of reverse transcription and RNA primer extension showed that hpol eta favors the add

87 ollectively suggest that during nonenzymatic RNA primer extension with a 5'-5'-imidazolium-bridged di

88 experimental reconstructions of nonenzymatic RNA primer extension yield a mixture of 2'-5' and 3'-5'

89 monomer addition as well as trimer-assisted RNA primer extension, allowing efficient copying of a va

90 e a deep-sequencing methodology for studying RNA primer extension.

91 fidelity, especially upon the second step of RNA primer extension.

92 us of the plus-strand primer from downstream RNA, primer extension significantly improved.

93 of the primase-catalyzed synthesis of short RNA primers followed by polymerase-catalyzed DNA synthes

94 A oligonucleotide containing the preannealed RNA primer, followed by incorporation of the complementa

95 For hepadnaviruses, the RNA primer for plus-strand DNA synthesis is generated by

96 PPT) to generate and subsequently remove the RNA primer for plus-strand DNA synthesis.

97 rocess of primer translocation, in which the RNA primer for the initiation of plus-strand DNA synthes

98 ependent RNA polymerase (RdRp) uses a capped RNA primer for transcription initiation.

99 DNA-dependent RNA polymerase that lays short RNA primers for DNA polymerases.

100 RNA primers for DNA replication are usually synthesized

101 itochondrial DNA heavy-strand origin provide RNA primers for initiation of mitochondrial DNA replicat

102 On duplexes containing the unique polypurine RNA primers for plus-strand DNA synthesis, the enzyme ca

103 polymerases called "primases" to synthesize RNA primers for the initiation of DNA replication.

104 Influenza virus polymerase uses capped RNA primers for transcription initiation in infected cel

105 c metalloenzyme responsible for synthesizing RNA primers for use during DNA synthesis.

106 uplexes, and the comprehensive hydrolysis of RNA primers formed during Okazaki fragment maturation.

107 t repeat 2 (DR2); (iii) DP-rcDNA exhibits an RNA primer-free 5' terminus of (+)strand DNA with either

108 has been unclear how Pol alpha hands over an RNA primer from Pri1 to Pol1 for DNA primer extension, a

109 aryotic and eukaryotic nucleases that remove RNA primers from lagging strand fragments during DNA rep

110 flap endonuclease-1 family nuclease, removes RNA primers from lagging strand fragments.

111 ase H is a 5' to 3' exonuclease that removes RNA primers from the lagging strand of the DNA replicati

112 cation of the genome requires the removal of RNA primers from the Okazaki fragments and their replace

113 DNA, a template switch is necessary for the RNA primer generated at DR1 to initiate plus-strand DNA

114 DNA synthesis is initiated at a purine-rich RNA primer generated by the RNase H activity of reverse

115 Polalpha extends the RNA primers generated by primase and provides a springbo

116 RNA primers generated by RNase H within the long termina

117 r a 5'-OH (DNA primer) or a 5'-triphosphate (RNA primer) group.

118 alkali and RNase treatment, suggesting that RNA primers had already been removed from the 5' end of

119 e apo, primer initiation, primer elongation, RNA primer hand-off from Pri1 to Pol1, and DNA extension

120 tethers Pol alpha and primase, facilitating RNA primer handover from primase to Pol alpha.

121 coupled an azide-modified VPg peptide to an RNA primer harboring a cyclooctyne [bicyclo[6.1.0]nonyne

122 allenge with 200 mm NaCl consists of an 8-nt RNA primer hybridized to a DNA template (T strand) that

123 lting complex can elongate the 3'-end of the RNA primer in a template-dependent manner with functiona

124 imase-helicase is essential for trapping the RNA primer in complex with the polymerase, and a unique

125 possibly implicating clamp loading onto the RNA primer in the mechanism of lagging strand polymerase

126 y subunit plays a role in the recognition of RNA primers in mtDNA replication, to recruit polgamma to

127 ctivation with likely roles in processing of RNA primers in Okazaki fragments during DNA replication,

128 Initiation and synthesis of RNA primers in the lagging strand of the replication for

129 We show that Poleta is able to extend RNA primers in the presence of ribonucleotides (rNTPs),

130 ines the extent and rate of synthesis of the RNA primers in vitro, direct evidence of the formation o

131 determining the physiological length of the RNA primers in vivo and the overall kinetics of primer s

132 rnary complexes with enzymes, RNA templates, RNA primers, incoming nucleotides, and catalytic metal i

133 and addition of one dNMP to the 3' end of an RNA primer increases activity 36-fold.

134 The mutant enzymes were able to bind to RNA primers, indicating that the defect in RNA priming w

135 This RNA primer initiates synthesis at one of two distinct si

136 e to extend the HIV-1 polypurine tract (PPT) RNA primer into (+) strand DNA, despite supporting the e

137 inimal in vitro system capable of processing RNA primers into ligatable DNA ends.

138 ermal enzymatic process where a short DNA or RNA primer is amplified to form a long single stranded D

139 processing which occurs after the initiator RNA primer is cleaved off, and released intact, by calf

140 For Okazaki fragments, the RNA primer is displaced into a 5' flap and then cleaved

141 eukaryotic Okazaki fragment processing, the RNA primer is displaced into a single-stranded flap prio

142 core polymerase and the requisite NTPs, the RNA primer is extended in a process that manifests most

143 The capped RNA primer is generated from host cell mRNA by the cap-s

144 We show that the final lagging RNA primer is not terminal but is randomly positioned ~7

145 Synthesis of the 7-10-nucleotide RNA primer is regulated by the C-terminal domain of the

146 An RNA primer is removed from each fragment before joining

147 s not impact the final DNA product since the RNA primer is replaced with DNA.

148 and extension, suggesting that the five-base RNA primer is sufficient for extension with dNTPs by DNA

149 The removal of RNA primers is essential for mitochondrial DNA (mtDNA) r

150 C-terminal helicase-binding domain modulated RNA primer length in a species-specific manner and produ

151 initiation, elongation, accurate counting of RNA primer length, primer transfer to Polalpha, and conc

152 the virus, whereas efficient extension from RNA primers located downstream from the PPT is predicted

153 DNA.DNA duplexes and can remove the pentamer RNA primers made by the T4 primase-helicase.

154 ises the additional possibility that DNA and RNA primers might be differentially recognized by the re

155 omplex), extended herpes primase-synthesized RNA primers much more efficiently than the viral polymer

156 eplication, since transcription generates an RNA primer necessary for initiation of mtDNA replication

157 h for gene expression and for generating the RNA primers needed for initiation of mtDNA synthesis.

158 of the endonuclease that produces the capped RNA primers needed for mRNA synthesis.

159 ect selection, extension, and removal of the RNA primers of (-)- and (+)-strand DNA synthesis (tRNA a

160 endent RNA polymerase that synthesizes short RNA primers of defined size for DNA polymerases.

161 Using RNA primers of different lengths, which were fully or pa

162 Correct removal of RNA primers of Okazaki fragments during lagging-strand D

163 nuclease 1 (FEN1) participates in removal of RNA primers of Okazaki fragments, several DNA repair pat

164 rities on rcDNA 5' termini, specifically the RNA primer on the (+)strand and the polymerase on the (-

165 polymerases engages the primase-helicase and RNA primer on the lagging strand of a model replication

166 Escherichia coli primase synthesizes RNA primers on DNA templates for the initiation of DNA r

167 Primases are enzymes that synthesize RNA primers on single-stranded DNA templates that are ex

168 vealed that the presence of 2'-5' linkage in RNA primer only modestly reduces pol II transcriptional

169 taining abasic lesions in either the PPT (+)-RNA primer or (-)-DNA template to locally remove nucleob

170 activated threo-nucleotide at the end of an RNA primer or in an RNA template results in only a modes

171 strand and a 5'-nuclease domain for cleaving RNA primers or damaged DNA strands.

172 f various structures, for example, to remove RNA primers or to produce 3' overhangs at telomeres or d

173 onstrated that S. aureus primase synthesized RNA primers predominately on templates containing 5'-d(C

174 orted oligoribonucleotide synthesis of short RNA primers (preferentially initiating synthesis on a dT

175 We examined the role of RNA primer-primase complexes left on the lagging ssDNA f

176 moval reaction and propose a novel model for RNA primer processing in human mitochondria.

177 Efficient RNA primer processing is a prerequisite for Okazaki frag

178 and DNA synthesis by DNA polymerase requires RNA primers produced by DNA primase.

179 2-mer representing the majority of the total RNA primers produced.

180 fficiently digest parental DNA while leaving RNA-primer protected nascent strands intact.

181 We find that primase tightly grips its RNA primer, protecting it from the action of other prote

182 were synthesized and tested along with short RNA primers ranging from two to five nucleotides.

183 ginning of DNA synthesis, when extending the RNA primer received from primase, Polalpha is more mutag

184 On substrates with an RNA primer recessed on a DNA template, the 5' end of the

185 f the C-terminus of the accessory subunit in RNA primer recognition, and previous observations that m

186 ry and sufficient for coupled DNA synthesis, RNA primer removal and DNA ligation.

187 c nuclease best known for its involvement in RNA primer removal and long-patch base excision repair.

188 ochondrial extract reduces its efficiency in RNA primer removal and LP-BER.

189 siae to elucidate the role of RNase H(35) in RNA primer removal during DNA replication and in mutatio

190 n of exonuclease 1 to flap endonuclease-1 in RNA primer removal during lagging strand DNA synthesis.

191 s suggest that the enzyme may be involved in RNA primer removal during minicircle replication.

192 Yeast Dna2 (yDna2) is essential in RNA primer removal during nuclear DNA replication and is

193 he mammalian nucleases RNase HI and FEN-1 in RNA primer removal has been substantiated by several stu

194 Several nucleases have been implicated in RNA primer removal in human mitochondria, however, no co

195 In eukaryotes, RNA primer removal is initiated by type 2 RNase H, which

196 It plays important roles in RNA primer removal of Okazaki fragments during DNA repli

197 findings, we suggest that three alternative RNA primer removal pathways of different efficiencies in

198 enzymes, we reconstitute for the first time RNA primer removal reaction and propose a novel model fo

199 n maintaining human genome stability through RNA primer removal, long-patch base excision repair, res

200 rad27Delta mutant, symptomatic of defective RNA primer removal.

201 een proposed for eukaryotic Okazaki fragment RNA primer removal.

202 role in polymerizing the formation of short RNA primers repeatedly on the lagging-strand template an

203 Bacterial DNA primase DnaG synthesizes RNA primers required for chromosomal DNA replication.

204 The capped RNA primers required for the initiation of influenza vir

205 s are responsible for the synthesis of short RNA primers required for the initiation of repetitive Ok

206 ete replication of linear chromosome ends by RNA primer-requiring DNA polymerases.

207 as a class of proteins that synthesize short RNA primers requisite for the initiation of DNA replicat

208 In the absence of an RNA primer, RET1 can use UTP itself to initiate nucleoti

209 st cases, the presence of an upstream DNA or RNA primer, separated from the monoribonucleotide-DNA se

210 he inhibitor is weakly incorporated into the RNA primer strand, and suppresses RNA replication in the

211 ult of the incorporated monophosphate in the RNA primer strand.

212 lains its low rate of incorporation into the RNA primer strand.

213 F-rUTP) and to link the monophosphate to the RNA primer strand.

214 bubble duplex in the absence of a hybridized RNA primer, suggesting that the binding of the core poly

215 DnaB helicase stimulated the second-order RNA primer synthesis activity of primase by over 5000-fo

216 e processivity factor, unwinding of DNA, and RNA primer synthesis all require conformational changes

217 40 origins in lieu of HSSB but inhibits both RNA primer synthesis and polymerase delta-catalyzed DNA

218 ntigen and is required for the initiation of RNA primer synthesis as well as processive elongation of

219 rylation of the Chk1 kinase are dependent on RNA primer synthesis by DNA polymerase alpha, and it has

220 We conclude that de novo RNA primer synthesis by DnaG and initial primer extensio

221 ity and large tumor antigen (T-ag)-dependent RNA primer synthesis by pol alpha-primase complex was ob

222 the unwound template strand but did require RNA primer synthesis by primase.

223 Mn(II) substitution leads to elevated RNA primer synthesis by T7 DNA primase.

224 nto a primosome to couple DNA unwinding with RNA primer synthesis for DNA replication.

225 Together, these findings support a model of RNA primer synthesis in which generation of the nascent

226 Here, we provide evidence that RNA primer synthesis is governed by a combination of the

227 some assembly process and sheds light on the RNA primer synthesis mechanism.

228 a complex event requiring repeated cycles of RNA primer synthesis, transfer to the lagging-strand pol

229 A in SV40 replication lies in T-ag-dependent RNA primer synthesis.

230 and, supporting a specific role for h-RPA in RNA primer synthesis.

231 e specificity of h-RPA is due to its role in RNA primer synthesis.

232 ry subunits, both of which are necessary for RNA primer synthesis.

233 recruited prior to origin DNA unwinding and RNA primer synthesis.

234 d) by its cognate helicase, DnaB(BA), during RNA primer synthesis.

235 fragments, is dependent on the frequency of RNA-primer synthesis.

236 p70-p180), which improves the utilization of RNA primers synthesized by herpesvirus primase on linear

237 t ATPase, primase, or RNA polymerase using a RNA primer-template and NTPs as substrates) but could st

238 bited robust RNA polymerase activity using a RNA primer-template and NTPs as substrates.

239 template complex was much higher for the DNA/RNA primer-template compared to DNA/DNA.

240 P while the Kd values determined for the DNA/RNA primer-template followed the order (-)SddCTP congrue

241 ze removal of a chain terminator from an RNA-RNA primer-template may show how slight changes in selec

242 structures of HCV polymerase in complex with RNA primer-template pairs.

243 loying a short, symmetrical, heteropolymeric RNA primer-template that we refer to as "sym/sub." Forma

244 The 3.2-A crystal structure of Polo on a DNA/RNA primer-template with bound deoxyribonucleotide revea

245 tructure of the ternary complexes of RT, DNA/RNA primer-template, and SddCTP analogues as well as imp

246 Photo-cross-linking of a RNA primer-template, the product of primer synthesis, co

247 Additionally, our approach for obtaining the RNA primer-template-bound structure of HCV polymerase ma

248 bonucleoside-5'-phosphorimidazolides with an RNA primer/template complex.

249 Comparing RNA primer-templates and DNA primer-templates of identic

250 ion of 5'-(32)P-end-labeled, heteropolymeric RNA primer/templates.

251 se transcription reactions from both DNA and RNA primer terminus, although its bypass efficiency is s

252 essed a much higher propensity to extend the RNA primer than the two-subunit polalpha (p180DeltaN-p70

253 nes the elements of the DNA template and the RNA primer that interact with p58C.

254 ative intermediate likely still retained the RNA primer that is attached to the 5' end of the plus st

255 he role of the sequence at the 5' end of the RNA primer that is outside of the DR sequence.

256 Each contains an initiator RNA primer that is removed prior to joining of the stran

257 rmediate as well as for generating the short RNA primer that is required for DNA second strand synthe

258 polymerase responsible for synthesis of the RNA primers that are elongated by the replicative DNA po

259 timulated by replicative helicase to produce RNA primers that are essential for DNA replication.

260 DNA primase synthesizes short RNA primers that are required to initiate DNA synthesis

261 Human DNA primase synthesizes short RNA primers that DNA polymerase alpha further elongates.

262 Human DNA primase synthesizes short RNA primers that DNA polymerase alpha then elongates dur

263 Bacteriophage T4 RNase H, which removes the RNA primers that initiate lagging strand fragments, has

264 tion machinery, responsible for synthesizing RNA primers that initiate leading and lagging strand DNA

265 al RNA polymerase, which produces the capped RNA primers that initiate viral mRNA synthesis, is compr

266 ase also prevented primase from synthesizing RNA primers that were longer than the template sequence.

267 oviral RT can bind either end of an annealed RNA primer, the 5'-end for degradation and the 3'-end fo

268 matic steps that control the synthesis of an RNA primer, the recycling of the lagging-strand DNA poly

269 By extending RNA primers, the lagging-strand polymerase restarts at s

270 In vitro, Fip1 blocks access of the RNA primer to an RNA binding site (RBS) that overlaps th

271 Primase catalyzes the synthesis of a short RNA primer to initiate DNA replication at the origin and

272 h T7 DNA polymerase and thereby delivers the RNA primer to the polymerase for the onset of DNA synthe

273 Addition of an RNA primer to yield a 4 base-pair post-translocated RNA:

274 mosomes requires a primase to generate short RNA primers to initiate genomic replication.

275 and its coupling to the primase synthesis of RNA primers to initiate Okazaki fragment synthesis; and

276 lar DNA, the major product, is made when the RNA primer translocates to the sequence complementary to

277 Moreover, purified vif mutant genomic RNA-primer tRNA complexes displayed severe defects in th

278 it did not affect their incorporation of IAP RNA, primer tRNAPhe (phenylalanine tRNA), or IAP Gag.

279 mostly been implicated in eliminating short RNA primers used for initiation of lagging strand DNA sy

280 er to determine the minimal requirements for RNA primer utilization by T7 DNA polymerase, we created

281 ates the nucleoside monophosphate (NMP) into RNA primer very efficiently (220 s(-1) at 25 degrees C).

282 Removal of the RNA primers was observed upon the addition of purified R

283 Using 3'-end-labeled PPT RNA primers, we also identified novel cleavage sites ahe

284 indicated that positions 4 and 6 within the RNA primer were important for recognition and cleavage b

285 ecificity, whereas equivalent lesions in the RNA primer were inhibitory.

286 In contrast, the helicase-stimulated RNA primers were from 10 to 14 nucleotides in length wit

287 trand synthesis involves the synthesis of an RNA primer which is removed in the last stage of replica

288 uire primase, an RNA polymerase making short RNA primers which are then extended by DNA polymerases.

289 Both pathways use the same RNA primer, which is capped and 18 or 19 nucleotides in

290 s the catalytic subunit that synthesizes the RNA primer, which is then extended by DNA polymerase alp

291 s the catalytic subunit that synthesizes the RNA primer, which is then utilized by Polalpha to synthe

292 re shorter by at least the size of the final RNA primer, which is thought to be located at extreme ch

293 stion of the capped fragments left resistant RNA primers, which enabled identification of zones of tr

294 de 5'-monophosphates and the synthesis of an RNA primer with a terminal MoNA nucleotide.

295 ymerase alpha (Pol alpha), which extends the RNA primer with deoxynucleotides.

296 an RNA primer/DNA template and extending an RNA primer with deoxynucleotides.

297 thymidine nucleotide generating a five-base RNA primer with the sequence 5'-AACCC.

298 to generate 10- to 14-nucleotide-long capped RNA primers with a 3' G residue.

299 tudies revealed that hPolepsilon(CD) extends RNA primers with approximately 3300-fold lower efficienc

300 that are recessed on a longer DNA template (RNA primers) yet binds to the 3' end of DNA primers.