ライフサイエンスコーパス: RNP

1 RNP delivery of BE3 confers higher specificity even than

2 RNP granules formed by ALS-linked mutant TDP-43 are more

3 RNP granules formed from wild-type TDP-43 show distinct

4 RNP in PDR can be identified at baseline and imaged seri

5 RNP-6 acts through TIR-1/PMK-1/MAPK signaling to modulat

6 RNP-loaded SMOF NPs induced efficient genome editing in

7 RNP-MaP also identified protein interaction networks con

8 RNP-MaP enables discovery and efficient validation of fu

9 RNP-MaP revealed that RNase P and RMRP, two sequence-div

10 RNP-MaP uses a hetero-bifunctional crosslinker to freeze

11 Analysis of more than 200 RNP particle tracks by Bayesian pattern recognition soft

12 Here, we show that TDP-43 RNP granules in axons of rodent primary cortical neurons

13 tes that the stabilized population of TDP-43 RNP granules in the proximal axon is less circular and s

14 ing events fail to occur: rotation of the 5S RNP, maturation of the peptidyl transferase center (PTC)

15 nce of NS1, both in the context of segment 7 RNPs reconstituted by plasmid transfection and in mutant

16 , proteins known to be present on additional RNP particles were identified, including Y box-binding p

17 improves Cas9 activity when delivered as an RNP.

18 reas NP associates with viral RNA to form an RNP complex that associates with the cytoplasmic face of

19 ex coacervation and subsequently triggers an RNP charge inversion, which promotes disassembly.

20 emerged as important regulators of LLPS and RNP granule dynamics, as they can directly weaken or enh

21 and phospho-regulates NP oligomerization and RNP assembly during infection.

22 ructures of spliced group II intron RNAs and RNP complexes have been characterized, structural insigh

23 .g., Cas9-sgRNA ribonucleoprotein (RNP), and RNP together with donor DNA (e.g., RNP + ssODN)).

24 into how PTMs regulate phase separation and RNP granule dynamics, in particular arginine (Arg)-methy

25 ctors, and enhances mRNA export via TREX and RNP, respectively, in addition to its role in facilitati

26 ltiple RNA-protein assemblies referred to as RNP granules, which are thought to form through multiple

27 le peptides combined with SpCas9 or AsCas12a RNP achieves editing of loxP sites in airway epithelia o

28 a role for dual specificity phosphatases at RNP particles and suggest that hYVH1 may affect a variet

29 Fragile X granules (FXGs) are axonal RNPs containing the fragile X related family of RNA bind

30 FXGs (Fragile X granules) are axonal RNPs present in a stereotyped subset of mature axons in

31 circuit requires not only identifying axonal RNPs and their mRNA cargoes, but also whether these RNPs

32 Bacterial RNP-bodies (BR-bodies) are a biomolecular condensate con

33 ally, we apply these advances to deliver BE3 RNPs into both zebrafish embryos and the inner ear of li

34 e, which may facilitate interactions between RNP granules.

35 hree different delivery platforms: biolistic RNP/DNA co-delivery; biolistic DNA delivery; and Agrobac

36 tial therapeutic avenues for protein and Cas RNP delivery to refractory airway epithelial cells.

37 protein or CRISPR-associated nuclease (Cas) RNP, allow rapid entry into cultured human ciliated and

38 It was demonstrated that a Cas9 RNP with a sequence modified tracrRNA enhanced indel for

39 evelopment of low cost, scalable CRISPR/Cas9 RNP affinity reagents as alternatives or augments to ant

40 onium chemistry for detection of CRISPR/Cas9 RNP by electrochemical, fluorescent, and colorimetric me

41 CRISPR/Cas9 RNP has been adapted for targeted genome editing within

42 Detection of the CRISPR/Cas9 RNP within biological samples is critical for assessing

43 nsing/quantification devices for CRISPR/Cas9 RNP.

44 sequence modified tracrRNAs to improve Cas9 RNP activity.

45 ediate efficient controlled delivery of Cas9 RNP in vitro and in vivo.

46 tracellular delivery and the release of Cas9 RNP into 293T cells and colorectal cancer (CRC) cells, t

47 ol for combined Cas9 ribonucleoprotein (Cas9 RNP)-mediated gene editing and lentiviral transduction t

48 effects, CP/Ad-SS-GD well interact with Cas9 RNP to form stable nanocomplex CP/Ad-SS-GD/RNP, which ca

49 convenient and efficient in delivering Cas9 RNPs for transient Cas9 expression and efficient genome

50 Furthermore, stabilizing Cas9 RNPs into nanoparticles with polyglutamic acid further i

51 he acquisition of new functions by catalytic RNPs.

52 assembly, or material properties of cellular RNP granules, such as stress granules or amyloid-like co

53 seq datasets for 60 human RBPs and RIP-ChIP (RNP immunoprecipitation-microarray) data for 69 yeast RB

54 and donor templates, we show that combining RNP and AAV donor delivery increases the efficiency of g

55 Here, we demonstrate that combining RNP delivery with naturally recombinogenic adeno-associa

56 However, combining RNPs with transgene-containing donor templates for targe

57 of spCas9 as an ribonuclearprotein complex (RNP) is considered the most safe and effective method to

58 protein/guide RNA ribonucleoprotein complex (RNP), while liposome-coating offers improved serum stabi

59 xport-competent ribonuclear protein complex [RNP]) to the active genes is facilitated by CBC.

60 Box C/D RNA protein complexes (RNPs) catalyze site-specific 2'-O-methylation of RNA wit

61 trating two kinds of RNA--protein complexes (RNPs), spliceosomal small nuclear (sn), and small CB-spe

62 achinery, large ribonucleoprotein complexes (RNPs) composed of the viral polymerase, genomic RNA and

63 s on Cas9/sgRNA ribonucleoprotein complexes (RNPs) to target and edit DNA.

64 e it is needed to bind TERT to form the core RNP enzyme.

65 be a common feature of transcription-coupled RNP assembly.

66 NC nanoplatform efficiently delivers CRISPR RNP complexes for in vitro and in vivo somatic gene edit

67 tes and archaea, is catalysed by the Box C/D RNP complex in an RNA-guided manner.

68 evidence that, in the context of the Box C/D RNP, the affinity of the catalytic module fibrillarin fo

69 In eukaryotic C/D RNP, the paralogous Nop58 and Nop56 proteins specificall

70 As all Box C/D RNPs are thought to adopt a similar structure, it remain

71 Our data show that all C/D RNPs share the same functional organization and mechanis

72 We have reconstituted active C/D RNPs with recombinant proteins of the thermophilic yeast

73 o the assembly specificity of eukaryotic C/D RNPs.

74 modification for both yeast and archaeal C/D RNPs.

75 due to the lack of carriers that can deliver RNPs systemically.

76 d lipid nanoparticles to efficiently deliver RNPs into cells and edit tissues including muscle, brain

77 developed carriers were also able to deliver RNPs to restore dystrophin expression in DMD mice and si

78 equences as well as an archetypal disordered RNP, fused in sarcoma, as model systems, we investigate

79 nally and, remarkably, structurally distinct RNPs, telomerase, and RNases P/MRP from unrelated progen

80 ile liquid-liquid phase separation may drive RNP granule assembly, the mechanisms underlying their su

81 litate selection of transformed cells during RNP delivery, a plasmid carrying a selectable marker gen

82 echanism to shape nascent RNA folding during RNP assembly.

83 Dysregulated RNP granules drive neuromuscular degenerative disease bu

84 Our results link dysregulated RNP granules to myocardial cellular pathobiology and hea

85 cs of Syp and the number of msp300:Syp:eIF4E RNP granules at the synapse, suggesting that these parti

86 gle mRNAs as they exit translation and enter RNP granules during stress.

87 ity of WF SS-OCTA to longitudinally evaluate RNP areas provides additional justification for adopting

88 e that during early stages of splicing, exon RNP complexes are highly dynamic with many proteins fail

89 flibercept dosing of PDR eyes with extensive RNP was not identified, and therefore the primary outcom

90 in C. elegans, we identified splicing factor RNP-6/PUF60 whose activity suppresses immunity, but prom

91 One method for RNP delivery into plant cells is the use of a biolistic

92 wever, primary transcription from pre-formed RNPs deposited by infecting particles is unaffected.

93 he translocation of longer 3' UTR mRNAs from RNPs to polysomes correlated with the production of new

94 RNP), and RNP together with donor DNA (e.g., RNP + ssODN)).

95 t hyaluronic acid (HA)-decorated CP/Ad-SS-GD/RNP nanocomplexes targeting mutant KRAS effectively inhi

96 9 RNP to form stable nanocomplex CP/Ad-SS-GD/RNP, which can be readily released in the reductive intr

97 readily induced using pairs of duplex guide RNPs targeted to a single chromosome.

98 ition of U2 to the spliceosome, and identify RNP rearrangements facilitated by PRP5 that are required

99 atalytic core of this biomedically important RNP enzyme.

100 GeoCas9 provides the foundation for improved RNP delivery in vivo and expands the temperature range o

101 performed without necessarily being bound in RNP complexes.

102 ic at baseline had no significant changes in RNP area 1 year after PRP.

103 were no statistically significant changes in RNP area on WF SS-OCTA images through 3 months after PRP

104 Our results show how compartmentalization in RNP granules differentially controls fates of mRNAs loca

105 prevalent in molecules not participating in RNP complexes.

106 rter 3' UTRs became increasingly enriched in RNPs from pachytene spermatocytes to round spermatids, a

107 nd the enrichment of shorter 3' UTR mRNAs in RNPs coincided with newly synthesized miRNAs that target

108 e extent of Orb6-mediated Sts5 assembly into RNP granules, we can modulate Efc25 protein levels, Ras1

109 cesses that can create an influx of RNA into RNP granules, such as transcription, can spatiotemporall

110 g sites, which, therefore, are targeted into RNPs for enrichment and delayed translation.

111 res of endogenously produced group II intron RNPs trapped in their pre-catalytic state.

112 ransport mechanisms, movement of GFP-labeled RNP particles in live A549 cells was recorded within 3 t

113 process for the nuclear export of very large RNPs and protein aggregates.

114 g the basis for establishing and maintaining RNP granules with distinct composition, are unknown.

115 The formation and dissolution of many RNP condensates are finely dependent on the RNA-to-RNP r

116 In contrast to many messenger RNP granules, such as processing bodies and stress granu

117 The eukaryotic cytosol contains multiple RNP granules, including P-bodies and stress granules.

118 its molecular basis for acting upon multiple RNPs remains unknown.

119 on of 280 pM RNP in reaction buffer and 8 nM RNP in biologically representative conditions.

120 ron, Tarrytown, NY) on retinal nonperfusion (RNP) in eyes with proliferative diabetic retinopathy (PD

121 presence or absence of retinal nonperfusion (RNP) in quadrants intersecting at the optic nerve head b

122 Areas of retinal nonperfusion (RNP) were drawn independently by 2 masked graders, and a

123 Here, we show that a novel RNP granule containing the mRNAs for axonemal dynein mot

124 res RBPs that bind to RNA as short as 30 nt, RNPs directly from animal tissue and can be used to simp

125 on for SGs as positive regulators of nuclear RNP granule assembly and suggests a role for disturbed S

126 The 7SK small nuclear RNP (snRNP), composed of the 7SK small nuclear RNA (snRN

127 with the core component of U1 small nuclear RNP in P. patens Genome-wide analyses demonstrated the i

128 ucleoproteins (RNPs) including small nuclear RNPs (snRNPs).

129 core subunit of the box C/D small nucleolar RNPs, Nop58p, is the target for R2TP function.

130 patients through 1 year, mean total area of RNP increased from 235 mm(2) to 266 mm(2) (P = 0.18) and

131 Areas of RNP identified on UWF FA images co-localized with RNP ar

132 gh many eyes demonstrated increased areas of RNP longitudinally (n = 24 [66.7%]), this was more commo

133 (ANOVA) tests were used to compare areas of RNP over time.

134 rk for investigating the dynamic assembly of RNP granules by phase separation at single-molecule reso

135 rces governs the heterotypic RLC behavior of RNP-RNA complexes.

136 rements consisted of areas and boundaries of RNP visualized using WF SS-OCTA and UWF FA.

137 One class of RNP granules consists of P bodies, which consist of nont

138 demonstrate that RNA, a primary component of RNP granules, can modulate the phase behavior of RNPs by

139 ZBP1 is a component of RNP transport particles and is known for its role in the

140 ation by inhibition of the nuclear import of RNP subunits, which not only uncovers a novel role of eE

141 , these results demonstrate the potential of RNP base editing of human HSPCs as a feasible alternativ

142 Transient presence of RNP molecules in cells can reduce undesirable off-target

143 to ranibizumab PRN may reduce progression of RNP in patients with BRVO, but a statistically significa

144 o disturbances in the material properties of RNP granules and consequent pathogenesis.

145 y disturbances in the material properties of RNP granules.

146 The hydrodynamic radius of RNP particles (86 nm) precludes simple diffusion through

147 response was identified with a reduction of RNP progression with monthly compared to quarterly dosin

148 lecular organization, and material states of RNP-RNA assemblies are controlled by a dynamic interplay

149 han directional motion, overall transport of RNP particles was dominated by hopping over the time int

150 One prominent type of RNP granule is the stress granule (SG), a dynamic and re

151 A second type of RNP granule, here named founder granules, contains oskar

152 At least two types of RNP granules populate the germ plasm, a cytoplasmic doma

153 rfusion in some cases by decreasing zones of RNP.

154 granules, can modulate the phase behavior of RNPs by controlling both droplet assembly and dissolutio

155 lipoMSN is used to deliver a combination of RNPs targeting these genes.

156 Despite the central role of RNPs during infection, the factors dictating where and w

157 The biochemical functions of the OLE RNP complex remain unknown, but are required for proper

158 the impact of gRNA structural alterations on RNP complex formation, R-loop dynamics, and endonuclease

159 mpact of the formation of the active site on RNP architecture.

160 ectively recruited to the surfaces of RNA or RNP condensates in vitro.

161 sequestered in ribonucleoprotein particles (RNPs) and thus subjected to delayed translation.

162 A cargos of the ribonucleoprotein particles (RNPs) that form the substrate for axonal translation.

163 is packed into ribonucleoprotein particles (RNPs) where RNA binding proteins ensure mRNA silencing a

164 d into axons in ribonucleoprotein particles (RNPs), complexes containing mRNAs and RNA binding protei

165 of a number of ribonucleoprotein particles (RNPs).

166 l measurements achieve a detection of 280 pM RNP in reaction buffer and 8 nM RNP in biologically repr

167 son of the catalytically activated precursor RNP to its previously reported spliced counterpart allow

168 nalysis of the catalytically inert precursor RNP demonstrated the structural impact of the formation

169 n networks analyzed by mutational profiling (RNP-MaP), a live-cell chemical probing strategy that map

170 nism for assembling liquid-like RNA/protein (RNP) bodies and other membrane-less organelles.

171 based delivery of Cas9-ribonuclear proteins (RNPs), we achieved near population-level genetic knockou

172 s and stress granules (SGs) are prototypical RNP granules localized exclusively in the nucleus and cy

173 Dysregulated sarcoplasmic RBM20 RNP granules displayed liquid-like material properties,

174 and granule size that collectively regulate RNP granule dynamics.

175 otein kinase C (PKC) family members regulate RNP assembly.

176 fluenza virus exploits host PKCs to regulate RNP assembly, a step required for the transition from pr

177 Consistent with its role in regulating RNP assembly, knockout of PKCdelta impairs virus infecti

178 Ribonucleoprotein (RNP) granules are biomolecular condensates-liquid-liquid

179 Ribonucleoprotein (RNP) granules are enriched in specific RNAs and RNA-bind

180 Ribonucleoprotein (RNP) granules are membrane-less organelles consisting of

181 Ribonucleoprotein (RNP) granules are membraneless organelles (MLOs), which

182 Ribonucleoprotein (RNP) granules are non-membrane-bound organelles that hav

183 Ribonucleoprotein (RNP) granules are RNA-protein assemblies that are involv

184 tivity of all possible 16 ribonucleoprotein (RNP) complexes (PB2, PB1, PA, NP) between CIV-H3N2 and p

185 OLE RNAs form a ribonucleoprotein (RNP) complex by partnering with at least two proteins, O

186 reagents into cells as a ribonucleoprotein (RNP) complex is the ability to edit genes without reagen

187 l, the intron RNA forms a ribonucleoprotein (RNP) complex with the intron-encoded protein (IEP), whic

188 able incorporation into a ribonucleoprotein (RNP) complex.

189 nomes when delivered as a ribonucleoprotein (RNP) complex.

190 ssential component of all ribonucleoprotein (RNP) granules.

191 Stress granules (SGs) are ribonucleoprotein (RNP) assemblies that form in eukaryotic cells as a resul

192 deliver BE3 and HF-BE3 as ribonucleoprotein (RNP) complexes into mammalian cells, establishing DNA-fr

193 T cells when delivered as ribonucleoprotein (RNP) complexes.

194 s9 machinery delivered as ribonucleoprotein (RNP) to the zygote has become a standard tool for the de

195 in and SpCas9 or AsCas12a ribonucleoprotein (RNP) delivery to cultured human well-differentiated airw

196 llowing injection of Cas9 ribonucleoprotein (RNP) complexes in the hippocampus, striatum and cortex.

197 sociated protein 9 (Cas9) ribonucleoprotein (RNP) complex is an RNA-guided DNA-nuclease that is part

198 essing (MRP), a catalytic ribonucleoprotein (RNP), recently reported by Lan et al. and Perederina et

199 ssembly of large, complex ribonucleoprotein (RNP) granules has become appreciated as an important reg

200 lationships within CRISPR ribonucleoprotein (RNP) enzymes and identify compatible chemistries for con

201 racellular vesicle (EV)-, ribonucleoprotein (RNP)-, and high-density lipoprotein (HDL)-specific miRNA

202 N57Q)-BE3 base editor for ribonucleoprotein (RNP) electroporation of human-peripheral-blood-mobilized

203 (CB) RNAs (scaRNAs) form ribonucleoprotein (RNP) complexes to mediate 2'-O-methylation of rRNAs and

204 hnRNPA2 is a human ribonucleoprotein (RNP) involved in RNA metabolism.

205 d the activity of the IDV ribonucleoprotein (RNP) complex, resulting in either attenuated or replicat

206 artitioning of mRNAs into ribonucleoprotein (RNP) granules supports diverse regulatory programs withi

207 Intracellular ribonucleoprotein (RNP) granules are membrane-less droplet organelles that

208 ytoplasmic, membrane-less ribonucleoprotein (RNP) granules enriched for RNA-processing enzymes, terme

209 re, affinity-purified MRP ribonucleoprotein (RNP) from HeLa cells cleaves the human pre-rRNA in vitro

210 ich are active as part of ribonucleoprotein (RNP) complexes, and "unbound," with physiological functi

211 especially in the form of ribonucleoprotein (RNP), remains elusive for clinical translation.

212 C), around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA.

213 r lysate resolved several ribonucleoprotein (RNP) complexes containing rRNAs and ribosomal proteins.

214 ineries (e.g., Cas9-sgRNA ribonucleoprotein (RNP), and RNP together with donor DNA (e.g., RNP + ssODN

215 monstrate that Cas9:sgRNA ribonucleoprotein (RNP)-mediated cleavage within a GATA1 binding site at th

216 t packaging of Cas9/sgRNA ribonucleoprotein (RNP).

217 rely on the multi-subunit ribonucleoprotein (RNP) complex Cascade to identify DNA targets and on the

218 The telomerase ribonucleoprotein (RNP) counters the chromosome end replication problem, co

219 The mechanisms underlying ribonucleoprotein (RNP) granule assembly, including the basis for establish

220 lizing a yet unidentified ribonucleoprotein (RNP) complex that is critical to the specificity of thes

221 The vault ribonucleoprotein (RNP), comprising vault RNA (vtRNA) and telomerase-associ

222 In that time, viral ribonucleoprotein (RNP) particles (nucleocapsids) travel from their initial

223 How and where ribonucleoprotein (RNP) transport granules that support this synthetic acti

224 section between MIB1 and ribonucleoproteins (RNPs) largely unexplored in mammalian cells.

225 delivering proteins and ribonucleoproteins (RNPs) to cells in vitro and mouse liver tissue in vivo w

226 ctively called EVs), and ribonucleoproteins (RNPs).

227 Cas9 system delivered as ribonucleoproteins (RNPs).

228 gh efficiency using Cas9 ribonucleoproteins (RNPs) containing either a sgRNA molecule or a synthetic

229 ting in hPSCs using Cas9 ribonucleoproteins (RNPs) in combination with AAV6-mediated DNA repair templ

230 plate interact with Cas9 ribonucleoproteins (RNPs) to shuttle the template to the nucleus, enhancing

231 In eukaryotic cells, ribonucleoproteins (RNPs) form mesoscale condensates by liquid-liquid phase

232 AD) by delivering CRISPR ribonucleoproteins (RNPs; Cas9 protein or Cpf1 protein and gRNA) into day-0.

233 c acids and gene editing ribonucleoproteins (RNPs) formulated with both commercially-available and ou

234 solation of cross-linked ribonucleoproteins (RNPs), but rather purifies them based entirely on their

235 lved in the formation of ribonucleoproteins (RNPs) including small nuclear RNPs (snRNPs).

236 nctional RNA aptamers or ribonucleoproteins (RNPs).

237 Ro60 ribonucleoproteins (RNPs), composed of the ring-shaped Ro 60-kDa (Ro60) prot

238 Such ribonucleoproteins (RNPs) can facilitate the high-fidelity introduction of s

239 tive conformation of the ribonucleoproteins (RNPs) and their higher-order assemblies were revealed.

240 anner and stabilized the ribonucleoproteins (RNPs) with a family of polypeptides bearing different ar

241 roup II introns into a dynamic, protein-rich RNP machinery.

242 ped exoribonuclease, forming a double-ringed RNP machine specialized for structured RNA degradation.

243 a domain that is a close tRNA mimic and Ro60 RNPs are often encoded adjacent to components of RNA rep

244 Studies of Ro60 RNPs in other bacteria hint at additional functions, sin

245 all nuclear (sn), and small CB-specific (sca)RNPs.

246 he com-modified sgRNA can package Cas9/sgRNA RNP into lentivirus-like particles via the specific inte

247 uence their functional repertoires and shape RNP evolution.

248 structurally related non-coding RNAs, share RNP networks and that network hubs define functional sit

249 r locus in the cell nucleus implicated in SL RNP biogenesis.

250 Soluble ICs containing Sm/RNP, an RNA Ag, activate human neutrophils to produce re

251 Eukaryotic box C/D small nucleolar (sno)RNPs catalyse the site-specific 2-O-methylation of ribos

252 Some RNP granules form, in part, through the formation of int

253 se effector proteins to generate specialized RNPs.

254 emporal and spatial distribution of specific RNP granules.

255 Cell type-dependent expression of specific RNP types with distinct mRNA cargos, such as FXGs, prese

256 et despite reduced dosage of target-specific RNP.

257 lowing injection of zebrafish eggs with such RNPs, virtually every copy of a targeted locus harbors a

258 rpin to the 5' end of a gRNA still supported RNP formation but produced a stable ~9 bp R-loop that co

259 MSN carrying both pcsk9 and angptl3-targeted RNPs, could not be reached with a single gene-editing ap

260 By delivering combinations of targeting RNPs in the same nanoparticle, synergistic effects on li

261 The telomerase RNP core enzyme is composed of a dedicated RNA subunit a

262 ecifically in Cajal bodies, where telomerase RNP complex assembly takes place.

263 variety of RNA-protein macrocomplexes termed RNP granules.

264 motif protein-20 (RBM20), we discovered that RNP granules accumulated abnormally in the sarcoplasm, a

265 Our results indicate that RNP formation was unaffected by any of our modifications

266 ctions were observed upon splicing among the RNP complexes.

267 ther, our data provide new insights into the RNP rearrangements and extensive exchange of proteins th

268 Characterization of the RNP complexes with MS-based protein identification and N

269 mmature virion and increases the size of the RNP globule, and exclusion of nucleocapsid from regions

270 of the dimerized 5'UTR at the surface of the RNP globule.

271 97% of the RNP particles jiggled within a small, approximately circ

272 in turn has limited the capabilities of the RNP-mediated genome editing toolbox.

273 ble marker gene can be co-delivered with the RNP to enrich for transformed/edited cells.

274 These RNP bionanoparticles generated Indels on different targe

275 d their mRNA cargoes, but also whether these RNPs are broadly conserved or restricted to only a few s

276 We found that this RNP granule contains the proteins Reptin and Pontin, los

277 Thus, the recruitment of mRNPs to RNP granules involves dynamic, stable and extended inter

278 ve effect, working downstream or parallel to RNP-6.

279 ndensates are finely dependent on the RNA-to-RNP ratio, giving rise to a windowlike phase separation

280 primary outcome measure was change in total RNP area (in square millimeters) from baseline to year 1

281 Mean total RNP increased from 207 mm(2) at baseline to 268 mm(2) (P

282 erformed side-by-side comparisons of the two RNP delivery methods across multiple gene loci and concl

283 ctions may be a general principle underlying RNP granule assembly.

284 t RNase L promotes the formation of a unique RNP complex that may have roles during the RNase L-media

285 we demonstrate that RLBs represent a unique RNP granule with a protein and RNA composition distinct

286 ld be rationalized for selected crRNAs using RNP stability and DNA target binding experiments.

287 ndensed phases driven by the IDRs of various RNP body proteins, including FUS, DDX4, and HNRNPA1.

288 Importantly, Rosetta-Vienna RNP-DeltaDeltaG establishes a framework for further impr

289 The resulting Rosetta-Vienna RNP-DeltaDeltaG method achieves root-mean-squared errors

290 ion of the heterotypic promoter by the viral RNP complex.

291 ne in the total number of quadrants in which RNP is present) at week 100 in the laser control, 2q4, a

292 ne in the total number of quadrants in which RNP is present) in the laser control, 2q4, and 2q8 group

293 ro at at least one site used in cells, while RNP isolated from cells with CRISPR-edited MRP loci lose

294 With RNP delivery of T. fusca Cascade and Cas3, we obtained 1

295 on factor 1 delta (eEF1D) may associate with RNP subunits, but its roles in IAV replication are uncle

296 able, and sometimes rigid, associations with RNP granules with stability increasing with both mRNA le

297 lthough translating mRNAs only interact with RNP granules dynamically, non-translating mRNAs can form

298 eEF1D interacted with RNP subunits polymerase acidic protein (PA), polymerase

299 dentified on UWF FA images co-localized with RNP areas visualized on WF SS-OCTA images.

300 er proteins that sequester these ends within RNPs, and with end modification pathways that protect th