ライフサイエンスコーパス: RRE

1 RRE recognition triggers a crucial coil-to-helix transit

2 RRE sequence diversity varied over the course of infecti

3 and DNAs, and viral RNAs including the HIV-1 RRE and TAR.

4 The precise secondary structure of the HIV-1 RRE has been controversial, since studies have reported

5 reagents for selective eradication of HIV-1 RRE mRNA.

6 ent CRM1 pathway, which is used by the HIV-1 RRE.

7 to the one previously reported for the HIV-1 RRE.

8 The 3D models for the HIV-2 RRE and folding intermediates are also presented, wherei

9 ation, the secondary structures of the HIV-2 RRE and two RNA folding precursors have been identified

10 nalysis collectively suggests that the HIV-2 RRE undergoes two conformational transitions before assu

11 follow-up at 52 weeks (HIIT, 39%; MCT, 25%; RRE, 34%; P=0.16).

12 PE, we have now determined that the wt NL4-3 RRE exists as a mixture of both structures.

13 cence titration assay revealed high-affinity RRE RNA binding by all 22 metal-chelate-Rev species, wit

14 c compounds, as a specific and high-affinity RRE-IIB binder which inhibits the interaction of the Rev

15 ssess whether BALF5 might be activated by an RRE-dependent mechanism, an Rta mutant (Rta K156A), defi

16 gesting this promoter can be activated by an RRE-dependent mechanism.

17 stem by an additional base pair, creating an RRE that was more responsive to lower concentrations of

18 was observed with the constructs lacking an RRE.

19 t (Rta K156A), deficient for DNA binding and RRE activation but competent for Zp/Rp activation, was u

20 While the CTE and RRE primarily enhance nucleocytoplasmic export, PPE-like

21 istic studies of naturally occurring Rev and RRE sequences are essential to understanding this system

22 Multiple Rev and RRE sequences were obtained using single-genome sequenci

23 Rev and RRE variants from each time point were subjected to func

24 protein expression in the absence of Rev and RRE.

25 (nucleocapsid) locally melts the TAR RNA and RRE-IIB RNA hairpins, whereas arginine-rich motif protei

26 y, but formed a super complex with Sam68 and RRE in vitro.

27 ementary DNA oligonucleotides to the TAR and RRE-IIB RNA hairpins, respectively.

28 Rta binding sites are likely functioning as RREs.

29 ve slightly lower RRE affinities, but better RRE specificities.

30 In SSKH cells, Rev failed to activate both RRE-mediated reporter gene [chloramphenicol acetyltransf

31 s and sugar-phosphate backbones of the bound RRE.

32 embly and budding were blocked, but changing RRE to PRE rescued HIV-1 Gag assembly and budding.

33 ion, and structural evolution of circulating RREs in this patient using plasma samples collected over

34 also reflected at the levels of cytoplasmic RRE-chloramphenicol acetyltransferase mRNAs, indicating

35 REBP can modestly enhance Rev-dependent RRE-linked reporter gene expression both independently a

36 t the first crystal structure of a Rev dimer-RRE complex, revealing a dramatic rearrangement of the R

37 nter in a protein and suggest that dinuclear RRE species, not mononuclear DNICs, may be the primary i

38 l layer requires technologies for disrupting RREs without perturbing cellular homeostasis.

39 transactivation of reporters containing each RRE showed that their promoter strengths in a transient-

40 lectrophoretic mobility shift assays of each RRE demonstrated that the highly purified Rdbd protein d

41 The early RRE was less functionally active than the late RRE, desp

42 EMSAs, using either the BTE or EBS-RRE probes, identified a specific protein-DNA complex, d

43 osslinking experiments with radiolabeled EBS-RRE and BTE oligonucleotides showed that these probes sp

44 ning GABP, we were able to show that the EBS-RRE preferentially binds Ets-1, while the BTE binds both

45 he level of relative replication efficiency (RRE) depends on the number and type of transcription fac

46 RiPP precursor peptide recognition element (RRE).

47 The viral Rev response element (RRE) adopts an "A"-like structure in which the two legs

48 Insertion of Rev response element (RRE) allows intron 2 to be retained, and beta-globin pro

49 of virion (Rev) to the Rev response element (RRE) and subsequent oligomerization in a cooperative man

50 point mutation in the Rev response element (RRE) at the bottom of stem-loop IIC.

51 HIV-1 Rev and the Rev response element (RRE) enable a critical step in the viral replication cyc

52 The HIV-1 Rev response element (RRE) is a 351-base element in unspliced and partially sp

53 The HIV-1 Rev response element (RRE) is a cis-acting RNA element characterized by multip

54 assembly of Rev on the Rev response element (RRE) is essential for the nuclear export of unspliced an

55 chelates to the HIV-1 Rev response element (RRE) mRNA have been synthesized.

56 NA containing either a Rev response element (RRE) or a Mason-Pfizer monkey virus (MPMV) constitutive

57 V-1 protein Rev on the Rev Response Element (RRE) regulates nuclear export of genomic viral RNA and p

58 been proposed for the Rev-response element (RRE) responsible for viral mRNA export, how it recruits

59 complexes composed of rev response element (RRE) ribonucleic acid (RNA) and multiple molecules of re

60 The HIV-1 Rev response element (RRE) RNA element mediates the nuclear export of intron c

61 oop II region of HIV-1 Rev response element (RRE) RNA enhanced binding of HIV-1 Rev protein to the RR

62 n homooligomer and the Rev response element (RRE) RNA to mediate nuclear export of unspliced viral mR

63 a homo-oligomer on the Rev response element (RRE) RNA.

64 al RNAs containing the Rev Response Element (RRE) through the Crm1 nuclear export pathway to the cyto

65 al Rev protein and the Rev response element (RRE), a structured element located in the Env region of

66 bunits assemble on the Rev Response Element (RRE), a structured region present in these RNAs, and dir

67 within viral RNA, the Rev response element (RRE), and escorts RRE-containing RNAs from the nucleus.

68 ral mRNAs encoding the Rev response element (RRE), thereby facilitating viral late gene expression.

69 virus (HIV) mRNAs, the Rev response element (RRE), to recruit the cellular nuclear export receptor Cr

70 transcripts harbor the Rev Response Element (RRE), which orchestrates the interaction with the Rev AR

71 or, unexpectedly, the Rev response element (RRE), which regulates the nuclear export of gRNAs and ot

72 ng regulatory RNA, the Rev response element (RRE), whose sequence changes over time during infection

73 P to mediate export of Rev response element (RRE)-containing human immunodeficiency virus (HIV) RNA,

74 zes with, HIV-1 Rev in Rev response element (RRE)-mediated gene expression and virus production.

75 ies with, HIV-1 Rev in Rev-response element (RRE)-mediated gene expression and virus replication.

76 v association with the Rev Response Element (RRE).

77 s, including the HIV-1 Rev response element (RRE).

78 V mRNAs containing the Rev response element (RRE).

79 d oligomerizing on the Rev Response Element (RRE).

80 ing frame 50 (ORF50)/Rta responsive element (RRE) and a TATA box.

81 cription activator (RTA) responsive element (RRE) are crucial cis-acting elements.

82 is dependent on the Ras responsive element (RRE) binding protein (RREB1), which negatively regulates

83 interactions with an RTA-responsive element (RRE) could complement the loss of one RBPjkappa binding

84 viral elements, the Rev-responsive element (RRE) of the human immunodeficiency virus (HIV), and the

85 ly homologous to the RTA-responsive element (RRE) of the PAN promoter.

86 ions between the HIV Rev-responsive element (RRE) RNA and the HIV regulatory protein Rev, are crucial

87 ng its corresponding Rev Responsive Element (RRE) RNA aptamer.

88 munodeficiency virus Rev-responsive element (RRE) RNA by the Rev protein is an essential step in the

89 the presence of the Rev responsive element (RRE) RNA to which it binds.

90 otein that binds the Rev responsive element (RRE) within the env gene of the HIV-1 RNA genome, activa

91 ously reported 16-bp RTA-responsive element (RRE), and the same mutation also both reduced RTA-mediat

92 As opposed to HIV's Rev-responsive element (RRE), the Rex-responsive element (RxRE) is present in al

93 SNV) facilitates Rev/Rev-responsive element (RRE)-independent expression of intron-containing human i

94 at also serves as an RTA-responsive element (RRE).

95 uclear export element (Rev-response element [RRE]) used by HIV-1 and EIAV with the hepatitis B virus

96 binding motifs and the R response elements (RRE) within oriLyt.

97 on by viral Rev and Rev-responsive elements (RRE).

98 s containing YUAAY RNA recognition elements (RREs).

99 o bind to specific RNA recognition elements (RREs).

100 RNA regulatory elements (RREs) are an important yet relatively under-explored fac

101 e RNA motifs known as Rev response elements (RREs) is required for transport of unspliced and partial

102 nate DNA sites termed Rta response elements (RREs).

103 a special focus on Ras responsive elements (RREs), the MAP kinases (Erks, p38 and JNK) and Ca2+-spec

104 d several regeneration-responsive enhancers (RREs), including an element upstream to inhibin beta A (

105 work suggests that changes in AP-1-enriched RREs are likely a crucial source of loss of regenerative

106 c description using reaction rate equations (RREs) is no longer accurate.

107 the Rev response element (RRE), and escorts RRE-containing RNAs from the nucleus.

108 l model of a Rev dimer bound to an essential RRE hairpin and to visualize the complete Rev-RRE RNP, d

109 s)(2)(NO)(4)], having a Roussin's red ester (RRE) formula, and that mononuclear DNICs account for onl

110 structurally related to Roussin's Red Ester (RRE, [Fe2 (NO)4 (Cys)2 ]) and Roussin's Black Salt (RBS,

111 eities in tempo (relative rate of evolution, RRE) and mode (selection pressure, Ka/Ks) in six organis

112 M10 resistance, which prompted us to examine RRE structure using a novel chemical probing strategy.

113 CT, or a recommendation of regular exercise (RRE).

114 uction correlated with the failure to export RRE-containing CAT mRNA and unspliced viral mRNAs to the

115 To optimize these derivatives for RRE specificity, a series of neomycin-acridine conjugate

116 sed placement of the 2 legs is essential for RRE function.

117 e of these mutants with a null phenotype for RRE activated the heterologous MS2 RNA target.

118 mutants that preserved the Rev response for RRE RNA localized to the nuclei; those with poor or no R

119 upport the essential role of the A shape for RRE function.

120 v binding and explains Rev's specificity for RRE-containing RNAs.

121 The presence of a functional RRE and a downstream TATA box suggested that this region

122 constants and chemical reactivity toward HIV RRE RNA have been determined and evaluated in terms of r

123 ins do not bind to the previously identified RREs.

124 Differences in RRE functional activity are attributable to specific cha

125 ty as a result of greater Shannon entropy in RRE stem-loop II, which is key to primary Rev binding.IM

126 An increase in RRE functional activity was observed over time, and a ke

127 r of Rev NES function and may play a role in RRE RNA transport during HIV infection.

128 d the functional contributions of individual RRE domains and now report that several domains contribu

129 analysis showed strong enrichment for known RREs but little or no enrichment for Rp or Zp, suggestin

130 sequencing (ChIP-seq) identified most known RREs and several novel Rta binding sites.

131 E was less functionally active than the late RRE, despite differing in sequence by only 4 nucleotides

132 bramycin and kanamycin A have slightly lower RRE affinities, but better RRE specificities.

133 ghest RNA and DNA affinities, but the lowest RRE specificity.

134 Mean RRE of the 18 endocannabinoid genes was significantly gr

135 ibited Sam68-mediated, but not Rev-mediated, RRE-dependent gene expression.

136 fferent CpG-containing BRLF1 binding motifs (RREs) in vitro or in vivo.

137 loops from HIV-1 Rev Response Element mRNA (RRE RNA) and ribosomal 16S A-site RNA (16S RNA) by metal

138 and Rec in HERV-K) and a region on the mRNA (RRE in HIV-1 and RcRE in HERV-K).

139 Rev binding, and also identifies non-native RRE conformational states as new targets for the develop

140 ing within the internal purine-rich bulge of RRE-IIB in a manner analogous to what has been observed

141 The rapid and sensitive detection of RRE-Rev interaction in complex biological systems repres

142 viral Rev protein induces nuclear export of RRE-containing RNAs, as required for virus replication.

143 al arginine-rich Rev peptide and stem IIB of RRE.

144 es that are not part of the internal loop of RRE stem IIB RNA, which was previously identified as the

145 a detailed understanding of the mechanism of RRE/rev association with implications for the rational d

146 ional assay, we have now analyzed a panel of RRE mutants.

147 specific hydrogen bonding for recognition of RRE, shape recognition, through contact with the sugar-p

148 hat rev initially binds to the upper stem of RRE IIB, from where it is relayed to binding sites that

149 Titrations of RRE-IIB with proflavine, monitored using (1)H NMR, demon

150 in situ high-content functional analysis of RREs.

151 t two classes of proflavine binding sites on RRE-IIB: a high-affinity site that competes with the Rev

152 s used to directly assess Rev "loading" onto RRE and its variants, indicating that this is unaffected

153 ration between them are required for optimal RRE function.

154 h the shortest linker length has the optimal RRE specificity.

155 turally intronless genes, but not the CTE or RRE from intron-containing genes, significantly enhanced

156 MCT at 60% to 70% of maximal heart rate, or RRE.

157 tures as closely related as the HIV-1 TAR or RRE elements.

158 me redundancy, to maintenance of the overall RRE shape.

159 ctly to the polyadenylated nuclear RNA (PAN) RRE motif, failed to bind to the RAP RRE and interfered

160 eptide's high-affinity mRNA binding partner, RRE stem loop IIB.

161 Selected patient RREs were also shown to have differences in Rev multimer

162 In all patients, RRE activity was more sensitive to sequence variation th

163 ory efficacy of proflavin on the Rev peptide-RRE binding, even in the presence of substantial levels

164 nsor for sensitively quantifying Rev peptide-RRE interaction and characterizing the potential inhibit

165 to what has been observed in the Rev peptide.RRE-IIB complex.

166 responsive element of the PAN promoter (pPAN RRE) was previously identified, and our data suggested d

167 ere mapped within a 16-bp region of the pPAN RRE by methylation interference assays.

168 , an extensive mutagenesis study on the pPAN RRE was carried out by using EMSAs and reporter assays.

169 ociation constant (K(d)) of Rdbd on the pPAN RRE was determined to be approximately 8 x 10(-9) M, sug

170 irect binding of full-length RTA to the pPAN RRE.

171 neity and measured its affinity for the pPAN RRE.

172 in the NOESY spectrum of the 2:1 proflavine.RRE-IIB complex indicate that the two proflavine molecul

173 ndicate that formation of the 2:1 proflavine.RRE-IIB complex stabilizes base pairing and stacking wit

174 ng interaction occurs with a 2:1 (proflavine:RRE-IIB) stoichiometry, and NOEs observed in the NOESY s

175 A (PAN) RRE motif, failed to bind to the RAP RRE and interfered with RRE-bound C/EBPalpha in EMSA exp

176 suggest that RTA transactivation of the RAP RRE is mediated by an interaction with DNA-bound C/EBPal

177 dithionite produces the one-electron-reduced RRE, having absorptions at 640 and 960 nm.

178 Rev-RRE assembly occurs via several Rev oligomerization and

179 agreement with previous models for HIV-1 Rev-RRE binding.

180 the full molecular structures of Rev and Rev-RRE complexes are not known.

181 RE hairpin and to visualize the complete Rev-RRE RNP, demonstrating that RRE binding drives assembly

182 and dissociation rates for the different Rev-RRE stoichiometries were determined.

183 Assembly of the functional Rev-RRE complex proceeds by cooperative oligomerization of R

184 tion is transferable and can replace HIV Rev-RRE-regulated expression of HIV gag.

185 trajectories recorded during individual Rev-RRE assembly reactions has revealed the microscopic rate

186 assembly and dissociation of individual Rev-RRE complexes in the presence or absence of DDX1 were ob

187 While a range of Rev-RRE activities were seen, the activity of cognate pairs

188 ich DDX1 promotes the nucleation step of Rev-RRE assembly.

189 rted in previous bulk kinetic studies of Rev-RRE association, indicating that oligomerization is an e

190 tering (SAXS) reveals two major steps of Rev-RRE complex formation, beginning with rapid Rev binding

191 in increased occurrence of higher-order Rev-RRE stoichiometries.

192 v monomer, thereby promoting the overall Rev-RRE assembly process.

193 or dominant-negative mutants suppressed Rev-RRE-function in the export of incompletely spliced HIV-1

194 Analysis of the Rev-RRE assembly pathway using SHAPE-Seq and small-angle X-r

195 ly accelerate the nucleation step of the Rev-RRE assembly process.

196 Formation of the Rev-RRE complex signals nucleocytoplasmic export of unsplice

197 Most of our understanding of the Rev-RRE regulatory axis comes from studies of lab-adapted HI

198 el of activity throughout infection, the Rev-RRE system can fluctuate, presumably to control replicat

199 interface that enhances association with Rev-RRE and poises NES binding sites to interact with a Rev

200 d translational enhancement, SNV RU5 and Rev/RRE were combined on a single gag RNA.

201 antisense inhibition of HIV mediated by Rev/RRE.

202 CRM-1 is also used to export Rev/RRE-dependent unspliced/ partially spliced HIV-1 RNAs.

203 m of the RRE promotes greater functional Rev/RRE activity compared to the four stem-loop counterpart.

204 However, the relevance of Sam68 in Rev/RRE function is not well defined.

205 Instead, Rev/RRE diverts RU5 gag RNA to the CRM1-dependent, LMB-inhib

206 -1 structural proteins in the absence of Rev/RRE is caused by inefficient accumulation of mRNA in the

207 eport that like snRNAs and snoRNAs, some Rev/RRE-dependent HIV-1 RNAs are TMG-capped.

208 ignificantly alter the efficiency of the Rev/RRE pathway.

209 ficking of the antisense RNA through the Rev/RRE pathway.

210 zymatic activities in the context of the Rev/RRE pathway.

211 iochemical activities with regard to the Rev/RRE system, while DDX3 differs.

212 Mechanistic studies indicated that the Rev/RRE-mediated inhibition did not involve either nuclear r

213 uality of CRM1's interactions with viral Rev/RRE ribonucleoprotein complexes in the nucleus.

214 ) into a truncated form of the RRE sequence (RRE-IIB) allowed the binding of an arginine-rich peptide

215 E resembles the HIV-1 nuclear export signal, RRE.

216 uorescently labeled Rev monomers to a single RRE molecule was visualized, and the event frequencies a

217 at both proteins can associate with a single RRE molecule.

218 The sequence-specific RRE RNA-Rev binding is essential for HIV-1 replication a

219 are necessary and sufficient for substantial RRE function, provided they are joined by a flexible lin

220 dependent viral gag-pol mRNAs bearing tandem RREs (GP-2xRRE), rescue virus particle production in mur

221 two RREs, thereby plausibly targeting tandem RREs present in many QKI-targeted transcripts.

222 their high-affinity binding to the targeted RRE mRNA following coupling to the Rev peptide, this cla

223 the complete Rev-RRE RNP, demonstrating that RRE binding drives assembly of Rev homooligomers into as

224 Our results suggest that RRE evolution during infection may be important for HIV

225 RE is subject to selection pressure and that RREs from later time points in infection tend to have hi

226 The RRE assembles a Rev oligomer that displays nuclear expor

227 accounts for the specificity of Rev for the RRE and thus the specific recognition of the viral RNA.

228 340, exhibits pronounced selectivity for the RRE RNA stem-loop from HIV-1.

229 ew compounds have a high specificity for the RRE.

230 the activation for MS2 RNA, but not for the RRE.

231 , can explain the specificity of Rev for the RRE.

232 However, two silent G->A mutations in the RRE (RRE61) confer RevM10 resistance, which prompted us

233 es with a major conformational change in the RRE and increased functional activity.

234 unds to various nucleic acids, including the RRE, tRNA, and duplex DNA.

235 a high-affinity site in stem-loop IIB of the RRE and proceeds rapidly by addition of single Rev monom

236 stem, we demonstrated that the region of the RRE and TATA box constitutes an ORF50/Rta-dependent prom

237 partially spliced viral RNA; binding of the RRE by the viral Rev protein induces nuclear export of R

238 onstrate that the five stem-loop form of the RRE promotes greater functional Rev/RRE activity compare

239 nopurine (2-AP) into a truncated form of the RRE sequence (RRE-IIB) allowed the binding of an arginin

240 hin the three-way junction of stem II of the RRE that is responsible for initial Rev binding.

241 e high-affinity stem-loop IIB segment of the RRE.

242 igh affinity for the Rev binding site on the RRE (K(d) <or= 10 nM), but few compounds have a high spe

243 that is otherwise unable to assemble on the RRE beyond a monomeric complex.

244 ultimerization of the HIV Rev protein on the RRE promote the nucleocytoplasmic export of incompletely

245 by cooperative oligomerization of Rev on the RRE scaffold and utilizes both protein-protein and prote

246 in enhancement of Rev oligomerization on the RRE that is correlated with an RNA structural change wit

247 uating the inhibitory effect of drugs on the RRE-Rev interaction.

248 r by promoting oligomerization of Rev on the RRE.

249 d by cooperative Rev-Rev interactions on the RRE.

250 model for Rev and its multimerization on the RRE.

251 at mapped to the envelope region outside the RRE.

252 DX1 acts as an RNA chaperone, remodeling the RRE into a conformation that is pre-organized to bind th

253 uggest that DDX1 targets Rev rather than the RRE to promote oligomeric assembly.

254 We previously reported that the RRE assumes an "A" shape in solution and suggested that

255 Here we show that the RRE controls the oligomeric state and solubility of Rev

256 We previously reported that the RRE is "A" shaped and suggested that this architecture,

257 In this study, we show that the RRE is subject to selection pressure and that RREs from

258 mutants, we show that DDX1 acts through the RRE RNA to specifically accelerate the nucleation step o

259 Thus, the RRE is not simply a passive scaffold onto which proteins

260 DX21 was shown to enhance Rev binding to the RRE in a manner similar to that previously described for

261 enhanced binding of HIV-1 Rev protein to the RRE in vivo and influenced nuclear export of RNA.

262 nity binding of multiple Rev monomers to the RRE is achieved on a much faster timescale than reported

263 ggest that RSG 1.2 binds more tightly to the RRE sequence than Rev by forming more base-specific cont

264 show here that initial binding of Rev to the RRE triggers RNA tertiary structural changes, enabling f

265 t the binding of an ORF50/Rta protein to the RRE was essential for ori-Lyt-dependent DNA replication.

266 We found that DDX21 can bind to the RRE with high affinity, and this binding stimulates ATPa

267 d the protein Rec-these are analogous to the RRE-Rev system in HIV-1.

268 Furthermore, when the RRE-driven antisense RNA was redirected to the Tap/Nxf1

269 The structure supports a model in which the RRE utilizes the inherent plasticity of Rev subunit inte

270 ssay, both K-Rta and ORF59 interact with the RRE and C/EBPalpha binding motifs within oriLyt in cells

271 ripts in vivo, interacting directly with the RRE and Rev in vitro, and promoting Rev oligomerization

272 yt DNA through RTA, which interacts with the RRE, as well as K8, which binds to a cluster of C/EBP bi

273 HIV-1 virus production was obtained with the RRE-driven antisense RNA.

274 ted with an RNA structural change within the RRE that persists even after dissociation of DDX1.

275 To determine the specificity of FMRP for the RREs, we performed quantitative in vitroRNA binding stud

276 We undertook a comparative study of the RREs of PAN RNA, ORF57, vIL-6, and Kpsn to understand ho

277 purified Rdbd protein directly bound to the RREs.

278 nsfections magnifies the difference in their RREs.

279 assess functional differences between these RRE 'conformers', we created conformationally locked mut

280 Conformational flexibility at this RRE region has been shown to be important for Rev bindin

281 Reduction of this RRE reaction product with sodium dithionite produces the

282 inds with better specificity and affinity to RRE than the Rev peptide.

283 HnRNP K did not bind to RRE-RNA directly, but formed a super complex with Sam68

284 the stoichiometry of Rev peptide binding to RRE can be accurately determined by using this single-QD

285 competes with the Rev peptide for binding to RRE-IIB (K(D) approximately 0.1 +/- 0.05 microM) and a w

286 proflavine competes with Rev for binding to RRE-IIB by binding as a dimer to a single high-affinity

287 de-RNA complexes of Rev and RSG 1.2 bound to RRE stem IIB have been solved and reveal gross structura

288 e parameter identifiability in comparison to RRE.

289 n uptake (P=0.70), but both were superior to RRE.

290 sly described the functional activity of two RREs found in circulating viruses in a patient followed

291 d that enables concurrent recognition of two RREs, thereby plausibly targeting tandem RREs present in

292 43/145 promoter through interaction with two RREs.

293 e N-terminal domain of SuiB adopts a typical RRE (RiPP recognition element) motif, which has been imp

294 ing replication, identify previously unknown RREs, such as one in BALF5p, and highlight the complexit

295 dramatic rearrangement of the Rev-dimer upon RRE binding through re-packing of its hydrophobic protei

296 high-throughput methods to identify various RREs in mRNAs that FMRP may bind to in vivo.

297 end-diastolic diameter changes compared with RRE were -2.8 mm (-5.2 to -0.4 mm; P=0.02) in HIIT and -

298 the Rev and RSG 1.2 peptides in complex with RRE stem IIB have been simulated to better understand on

299 d to bind to the RAP RRE and interfered with RRE-bound C/EBPalpha in EMSA experiments.

300 bits the interaction of the Rev peptide with RRE-IIB.