ライフサイエンスコーパス: RT-PCR

1 RT-PCR and transcription factor binding analysis identif

2 RT-PCR detects RNA, not infectious virus; thus, its abil

3 RT-PCR of cell lysates transfected with rdLRP demonstrat

4 RT-PCR tests took an average of 2.5 days +/- 0.7 to prov

5 RT-PCR using viral specific primers revealed a lower inf

6 tion compared to species-specific SARS-CoV-2 RT-PCR assays.

7 e the relationship between E gene SARS-CoV-2 RT-PCR cycle threshold (Ct) values from respiratory samp

8 he performance of high-throughput SARS-CoV-2 RT-PCR systems.

9 ve cross-sectional study, we took SARS-CoV-2 RT-PCR-confirmed positive samples and determined their a

10 f alternative viral infections in SARS-CoV-2 RT-PCR-negative PUIs (n = 30) and viral coinfections in

11 n = 30) and viral coinfections in SARS-CoV-2 RT-PCR-positive PUIs (n = 45).

12 Among SARS-CoV-2 RT-PCR-positive PUIs, lower cycle threshold (C(T) ) valu

13 From March 15 to June 1, 2020, 250 RT-PCR confirmed COVID-19 patients were studied with low

14 retrospective multi-institutional study, 254 RT-PCR verified COVID-19+ patients with at least one CXR

15 Of the 376 RT-PCR tests evaluated, 200 (53%) were positive and 176

16 e causative virus was later sequenced from a RT-PCR-positive individual and assessed using phylogenet

17 of L-DNAs to develop a multiplexed Adaptive RT-PCR instrument and assay for the detection of Zika, d

18 diograph categorization was compared against RT-PCR results to determine the utility of chest radiogr

19 We also show that all RT-PCR confirmed COVID-19 patients tested in our study d

20 Among RT-PCR-confirmed infections in adults, pre-existing anti

21 Among RT-PCR-confirmed infections in children, hemagglutinatio

22 A total of 104 individuals had an RT-PCR-positive viral test with a cycle threshold (C(T)

23 ed before a dialysis session and received an RT-PCR test; 79 (22.2% of the total study population) te

24 MILAN technique) followed by image analysis, RT-PCR and shotgun proteomics.

25 istry, immunofluorescence, Western blot, and RT-PCR were performed in highly purified peripheral bloo

26 pating centers; 1224 were screened by CT and RT-PCR and 869 by chest CT only.

27 nt preoperative screening using chest CT and RT-PCR before elective or emergency surgery under genera

28 creening using a combination of chest CT and RT-PCR was 1.5% [95% confidence interval (CI): 0.8-2.1].

29 hy, histomorphometry, in vitro cultures, and RT-PCR.

30 in the ischemic cortex of GPR37 KO mice, and RT-PCR identified an enrichment of M1-type microglia or

31 NA and RNA viruses using a real-time PCR and RT-PCR.

32 ranscriptase-quantitative PCR (RT-Q-PCR) and RT-PCR amplicon sequencing, provide a convenient, target

33 antly associated with rat IgG positivity and RT-PCR positivity (P = .03 and P = .006, respectively).

34 ne expression was analyzed using RNA-Seq and RT-PCR.

35 s, using immunostaining, RNA sequencing, and RT-PCR.

36 In addition, immunofluorescence staining and RT-PCR showed downregulation of molecules involved in th

37 ons tested for SARS-CoV-2 by BinaxNOW TM and RT-PCR in a community setting, rapid assay sensitivity w

38 dated immune epitopes, promising antibodies, RT-PCR and sequencing primers, CRISPR guides (from resea

39 rform diagnostic tests using methods such as RT-PCR or reverse transcription loop mediated isothermal

40 800 and Panther Fusion, and their associated RT-PCR assays, with a collection of 389 nasopharyngeal s

41 rs/1000 tests (with reductions throughout at RT-PCR sensitivity >=65%), and absences by -0.5 to +3.6

42 y, 131 (70%) were positive for SARS-CoV-2 by RT-PCR or antibody testing, and 164 (88%) were hospitali

43 (3-75) years, with detectable SARS-CoV-2 by RT-PCR.

44 omeprazole and esomeprazole were assessed by RT-PCR and RNA sequencing.

45 Selected identified genes were confirmed by RT-PCR.

46 of ET-1 mRNA in heart tissue was detected by RT-PCR.

47 ntoxigenic isolates tested, were detected by RT-PCR.

48 ion, among which the viral gene detection by RT-PCR has been found as the most reliable technique.

49 xpression of topo IIalpha and topo IIbeta by RT-PCR and its relationship to immunophenotype (IP) and

50 rome coronavirus 2 (SARS-CoV-2) infection by RT-PCR, immunohistochemical staining of kidney biopsy sa

51 h July, 2020, 7,335 of whom were positive by RT-PCR or antigen testing, and who had at least 15 of 20

52 and tested nasopharyngeal (NP) specimens by RT-PCR two or more times during a 14-day period.

53 POP2, CARD16, CARD18, TRIM16, and TRIM20 by RT-PCR.

54 ITGAX) renal lesions that were validated by RT-PCR and IHC.

55 l proliferation for subsequent validation by RT-PCR.

56 The finding of YFV by RT-PCR in five Callithrix monkeys who were all YFV-negat

57 2017), urine samples were tested for ZIKV by RT-PCR from all women attending the high-risk pregnancy

58 Single cell RT-PCR on lung-specific vagal afferent neurons revealed

59 ssion of 5-HT(3) receptors using single cell RT-PCR, while sensory neuronal and urothelial responses

60 By combining RT-PCR, immunohistochemistry, isolated tubule perfusion,

61 patients were reviewed along with concurrent RT-PCR tests.

62 All consecutive patients with confirmed RT-PCR testing from April to June 2020 were invited to p

63 urveillance were genotyped with conventional RT-PCR based methods and whole genome sequenced using th

64 This new Corynebacterium RT-PCR method provides a rapid tool to screen isolates a

65 ur study was to determine how EV and PeV CSF RT-PCR testing impacted the duration of antibiotic use a

66 patients for COVID-19 using either chest CT, RT-PCR or both, due to the risk for worsened surgical ou

67 ve, may fill many of the gaps in the current RT-PCR testing regime, especially in low- and middle-inc

68 lammation was evaluated with flow cytometry, RT-PCR, MultiPlex, and immunofluorescence staining.

69 cally, we include a SWEET promoter database, RT-PCR primers for detecting SWEET induction, engineered

70 in solids using one-step digital droplet (dd)RT-PCR than with two-step RT-QPCR and two-step ddRT-PCR,

71 WT) and I38T mutant were assessed by digital RT-PCR.

72 Initial experiments used two pathway-focused RT-PCR gene arrays ("wound healing" and "neurogenesis")

73 For RT-PCR analyses, samples from 15 subjects with diabetes

74 for chest CT and 1.1% (95% CI: 0.6-1.7) for RT-PCR; the incremental yield of chest CT was 0.4%.

75 ins the gold standard of tear collection for RT-PCR assay.

76 daily nasopharyngeal/throat swabs (NTSs) for RT-PCR testing.

77 Vero cell infectivity was only observed for RT-PCR Ct < 24 and STT < 8 days.

78 s curve (AUC) was compared with results from RT-PCR and clinical diagnosis of COVID-19.

79 ed and characterized by transcriptome-guided RT-PCRs and Sanger sequencing.

80 D-19 suspected on presentation); group 2 had RT-PCR results obtained after CT scans were read (COVID-

81 range, 29-94]) were included, of whom 45 had RT-PCR-confirmed COVID-19.

82 ith 7500 patients entered; 2652 did not have RT-PCR test results or had unknown or excess delay betwe

83 lecular techniques for CTV detection include RT-PCR and RT-qPCR.

84 I-coded hospitalizations, 1064 (6%) included RT-PCR testing for influenza viruses, 614 (58%) of which

85 Sensitivity of initial RT-PCR (91% [95% CI: 83-97%]) was higher than baseline C

86 Care must be taken in interpreting RT-PCR tests for SARS-CoV-2 infection-particularly early

87 HPV16 circE7 is detectable by both inverse RT-PCR and northern blotting of HPV16-transformed cells.

88 were documented through positive Legionella RT PCR at 52 and 53 days (cycle threshold detection of 2

89 tomato RNAi lines was confirmed by stem-loop RT-PCR.

90 d individuals, G614 is associated with lower RT-PCR cycle thresholds, suggestive of higher upper resp

91 ood and developed PROMIDISalpha, a multiplex RT-PCR/next-generation sequencing assay, to evaluate a s

92 were genotyped using a hemi-nested multiplex RT-PCR.

93 Moreover semi-multiplex RT-PCR technique was carried out in order to determine t

94 Here, using a very sensitive multiplex RT-PCR assay, we screened 1,645 formalin-fixed paraffin-

95 F (P = 0.050) were higher and nasopharyngeal RT-PCR cycle threshold values lower (P = 0.010) in patie

96 and compare tests such as the CDC 2019-nCoV RT-PCR Diagnostic Panel, Cellex's qSARS-CoV-2 IgG/IgM Ra

97 One positive or two negative RT-PCR tests for COVID-19 were considered the gold stand

98 reaction [RT-PCR] results, 231 with negative RT-PCR results).

99 California and Michigan compared to a nested RT-PCR genotyping assay and the ISO 15216-1 rRT-PCR meth

100 Ninety RT-PCR SARS-CoV-2-positive samples were incubated on Ver

101 to compare the baseline strategy of (S0) no RT-PCR testing of workers to testing workers (S1) with C

102 Analysis of RT-PCR positivity probability showed that asymptomatic p

103 tion should not be ruled out on the basis of RT-PCR alone, and the clinical and epidemiologic situati

104 ring the current pandemic, the deployment of RT-PCR tests has been extremely slow, and key reagents s

105 However, the proportion of RT-PCR-confirmed COVID-19 cases in the categories atypic

106 results and there is currently a shortage of RT-PCR test kits, underscoring the urgent need for alter

107 d to compare the accuracy of CT with that of RT-PCR.

108 9 contact history, oxygen therapy, timing of RT-PCR testing, and likely alternative diagnosis.

109 acute respiratory syndrome coronavirus 2 on RT-PCR assay from a nose or throat swab.

110 Many nucleic acid tests based on RT-PCR were developed, each with different techniques, s

111 ltidisciplinary group of clinicians based on RT-PCR, COVID-19 contact history, oxygen therapy, timing

112 with 1484 (12%) of these cases confirmed on RT-PCR assay.

113 rwent both initial chest CT and at least one RT-PCR test within 48 hours were included.

114 In the 75 patients, RT-PCR analysis of tears showed positive results in 18 p

115 ds to determine toxigenicity, real-time PCR (RT-PCR) provides a faster method to detect the toxin gen

116 e performed more than 150,000 real-time PCR (RT-PCR) tests, with many more to come.

117 ography-mass spectrometry and real-time PCR (RT-PCR), respectively.

118 ation serogrouping (SASG) and real-time PCR (RT-PCR).

119 were further validated using real time PCR (RT-PCR).

120 be quantified by reverse transcriptase PCR (RT-PCR) assays with single-copy sensitivity.

121 -initial positive reverse transcriptase PCR (RT-PCR) result were 92.9% (78/84), 88.1% (74/84), 97.6%

122 e, and SARS-CoV-2 reverse transcriptase PCR (RT-PCR) results were compared.

123 A real-time reverse transcriptase PCR (RT-PCR) screening revealed that 8 tissue samples from 7

124 Reverse transcription PCR (RT-PCR) and sequencing revealed that all US isolates and

125 cs for SARS-CoV-2 reverse transcription-PCR (RT-PCR) and highlight the importance of having multiple

126 ercial SARS-CoV-2 reverse transcription-PCR (RT-PCR) assays have received Emergency Use Authorization

127 stage, real-time reverse transcription-PCR (RT-PCR) assays remain the molecular test of choice for t

128 ar assays such as reverse transcription-PCR (RT-PCR) have increased the sensitivity of viral detectio

129 Traditional reverse transcription-PCR (RT-PCR) identification of known viruses was unsuccessful

130 ses two real-time reverse transcription-PCR (RT-PCR) platforms, the Roche Cobas SARS-CoV2 and the Cep

131 tients who tested reverse transcription-PCR (RT-PCR) positive for SARS-CoV-2 for whom 689 excess seru

132 ger sequencing of reverse transcription-PCR (RT-PCR) products indicates that there are viral transcri

133 logical and viral reverse transcription-PCR (RT-PCR) testing along with repeat testing after return t

134 ne CSF EV and PeV reverse transcription-PCR (RT-PCR) testing was performed during January 2011 to Dec

135 us 2 (SARS-CoV-2) reverse transcription-PCR (RT-PCR) testing.

136 scripts by TaqMan reverse transcription-PCR (RT-PCR), localization of infection by RNAscope in situ h

137 response in 24/24 reverse transcription-PCR (RT-PCR)-confirmed COVID-19 cases sampled at >14 days pos

138 ied CDC 2019-nCoV reverse transcriptase PCR [RT-PCR] assay with RNA extraction by eMag [bioMerieux] a

139 WHO-recommended reverse transcriptase PCRs (RT-PCRs).

140 Routine EV/PeV RT-PCR testing of cerebrospinal fluid (CSF) samples in c

141 cyte differentiation, regulation of 15-PGDH, RT-PCR, and Western blot, were performed using periphera

142 -19 community prevalence, up to ~6% positive RT-PCR was found for a daily hospital admission rate >1.

143 e included, of whom 536 (50%) had a positive RT-PCR result and 137 (13%) of whom were considered to h

144 for SARS-CoV-2 RNA, and 50.0% had a positive RT-PCR result.

145 ded hospitalised patients who had a positive RT-PCR test for severe acute respiratory syndrome corona

146 al diagnosis of COVID-19, without a positive RT-PCR test.

147 e asymptomatic at the date of first positive RT-PCR collection; two (4%) had preceding symptoms that

148 mptom profiles on the date of first positive RT-PCR test and described progression of symptoms over t

149 ported symptoms on the day of first positive RT-PCR test were upper respiratory (n=32, 68%) and neuro

150 Five patients showed positive RT-PCR results by all 3 methods (mean Ct value, 25.24 +/

151 he subgroup of 58 participants with positive RT-PCR and CT findings, ground-glass opacities were pres

152 ated in a subgroup of patients with positive RT-PCR and CT findings.

153 of symptomatology in patients with positive RT-PCR results from tears was 5 days (range, 4-9 days).

154 tandard deviation]; 61 men, 53 with positive RT-PCR results).

155 was 0.91 (95% CI: 0.85, 0.97) for predicting RT-PCR outcome and 0.95 (95% CI: 0.91, 0.99) for clinica

156 In our population with previous RT-PCR confirmed infection, approximately one in 16 pers

157 Quantitative RT-PCR analysis also showed an increase in the expressio

158 Quantitative RT-PCR analysis confirmed increased transcription of IL-

159 Quantitative RT-PCR and Northern blotting studies showed reduced or l

160 Quantitative RT-PCR and Western blot analysis of sciatic nerve tissue

161 Quantitative RT-PCR experiments with inhibitors of PI3K and STAT5 sho

162 Quantitative RT-PCR is the most extensively used approach for gene ex

163 Quantitative RT-PCR of polg2, fis1, opa1, sod1/2 and bcl2a from isola

164 Quantitative RT-PCR was used to validate a common signature of GA str

165 RNA-Seq and quantitative RT-PCR analyses revealed increased expression of genes a

166 oughput expression analysis and quantitative RT-PCR analysis was further employed to identify the fam

167 tion assays, Rho activation and quantitative RT-PCR assays, we investigated the mechanism by which CS

168 Immunohistochemistry and quantitative RT-PCR showed S100A8 & A9 expression was significantly u

169 ured using Western blotting and quantitative RT-PCR with subsequent matrix metalloproteinase (MMP) ac

170 ined by microarray analysis and quantitative RT-PCR, and expression of proteins was measured by ELISA

171 ne expression by RNA-arrays and quantitative RT-PCR, and metabolite levels in liver and plasma by HPL

172 ed gene silencing, RNA-Seq- and quantitative RT-PCR-based transcriptomics, and ultra-high-performance

173 nd microglia by qualitative and quantitative RT-PCR.

174 istry, electron microscopy, and quantitative RT-PCR.

175 analyzed by flow cytometry and quantitative RT-PCR.

176 red results from a CDC-approved quantitative RT-PCR (RT-qPCR) assay performed in a state testing lab,

177 ecessary to detect the virus by quantitative RT-PCR (RT-qPCR), the most robust, sensitive, and specif

178 (HUVECs) in vitro, followed by quantitative RT-PCR analysis and OLINK Proteomics.

179 confirmed to be up-regulated by quantitative RT-PCR and immunohistochemical staining.

180 croarray analysis, validated by quantitative RT-PCR, indicated down-regulation of the hypoxia-associa

181 ression of ACE2 was measured by quantitative RT-PCR.

182 First, quantitative RT-PCR and flow cytometry analyses revealed that human m

183 Results from quantitative RT-PCR and CRISPR/Cas9-aided gene knockout experiments i

184 cell isolation, immunoblotting, quantitative RT-PCR, and liquid chromatography-tandem mass spectromet

185 ng isolated MV, immunoblotting, quantitative RT-PCR, FACS analysis, and enzymatic assays, we show tha

186 gene silencing, immunoblotting, quantitative RT-PCR, promoter-reporter assays, and reactive oxygen sp

187 genes for gene normalisation in quantitative RT-PCR studies using endometrial biopsies obtained from

188 chain reaction (RT-PCR), novel quantitative RT-PCR primers/probe were developed, and whole genome se

189 cluding protein overexpression, quantitative RT-PCR, immunofluorescence, and various biochemical assa

190 ression was assayed at the RNA (quantitative RT-PCR) and protein (western blotting) levels.

191 Real-time quantitative RT-PCR and RNAscope in situ hybridization assays were us

192 We used quantitative RT-PCR and histopathologic analyses to evaluate inflamma

193 Using Quantitative RT-PCR and Cyquant assay, we showed that miR-124 protect

194 roRNA and mRNA expression using quantitative RT-PCR.

195 erformed and confirmed by using quantitative RT-PCR.

196 s from allergic asthma by using quantitative RT-PCR.

197 RNA sequencing (RNA-seq), with quantitative RT-PCR validation of immune and barrier biomarkers.

198 hes employing FACS coupled with quantitative RT-PCR, a validated GLP-1R antibody, and flow cytometry

199 deep RNA-Seq complemented with quantitative RT-PCR, we found that feeding causes substantial and tra

200 rse transcription-polymerase chain reaction (RT-PCR) analysis or serological testing was used to conf

201 ens for real-time polymerase chain reaction (RT-PCR) and immunoglobulin M enzyme-linked immunosorbent

202 rse transcriptase polymerase chain reaction (RT-PCR) and other variables which may influence the inte

203 rse transcriptase polymerase chain reaction (RT-PCR) are being used to rule out infection among high-

204 rse transcription polymerase chain reaction (RT-PCR) are the gold standard, during the current pandem

205 rse-transcription polymerase chain reaction (RT-PCR) assay and a clinical reference standard establis

206 rse transcriptase-polymerase chain reaction (RT-PCR) assay of nasal and pharyngeal swabs.

207 rse transcription polymerase chain reaction (RT-PCR) assays were compared together and with the final

208 rse transcription-polymerase chain reaction (RT-PCR) data showed that both M2 and M13 (at 1.0 mug/mL)

209 rse transcription polymerase chain reaction (RT-PCR) during April 7-May 6, 2020.

210 rse transcriptase polymerase chain reaction (RT-PCR) examination of abdominal fluid was negative for

211 rse-transcription polymerase chain reaction (RT-PCR) has become the primary method to diagnose viral

212 rse-transcription polymerase chain reaction (RT-PCR) in Australia, Canada, Israel, and the United Sta

213 rse transcription polymerase chain reaction (RT-PCR) results obtained before CT scan reading (COVID-1

214 rse transcriptase polymerase chain reaction (RT-PCR) test is routinely used.

215 rse transcriptase polymerase chain reaction (RT-PCR) test result and who were tested for IgG antibodi

216 rse transcription polymerase chain reaction (RT-PCR) test, but chest CT may play a complimentary role

217 rse transcription polymerase chain reaction (RT-PCR) testing for SARS-Cov-2 nucleic acid.

218 rse-transcription polymerase chain reaction (RT-PCR) testing.

219 rse-transcription polymerase chain reaction (RT-PCR) tests are accurate but costly, which makes the r

220 rse transcription-polymerase chain reaction (RT-PCR) was performed (mean, 62 years +/- 16 [standard d

221 rse-transcriptase polymerase chain reaction (RT-PCR), antibody testing, or exposure to persons with C

222 rse-transcription polymerase chain reaction (RT-PCR), novel quantitative RT-PCR primers/probe were de

223 rse transcription-polymerase chain reaction (RT-PCR), particularly after the second week of infection

224 rse transcription polymerase chain reaction (RT-PCR), which can have good sensitivity and excellent s

225 rse transcription polymerase chain reaction (RT-PCR)-based gene expression analyses of molecules that

226 rse-transcription polymerase chain reaction (RT-PCR)-confirmed COVID-19 in each of the four chest CT

227 rse transcriptase-polymerase chain reaction (RT-PCR)-confirmed influenza virus A(H1N1)pdm infections

228 rse-transcription polymerase chain reaction (RT-PCR)-confirmed SARS-CoV-2 infection, collecting clini

229 rse-transcriptase polymerase chain reaction (RT-PCR).

230 rse transcriptase polymerase chain reaction (RT-PCR).

231 rse transcription polymerase chain reaction (RT-PCR).

232 rse transcription polymerase chain reaction (RT-PCR).

233 rse-transcriptase-polymerase-chain-reaction (RT-PCR) assay.

234 rse transcription polymerase chain reaction [RT-PCR] results, 231 with negative RT-PCR results).

235 Modern HCV-RNA RT-PCR assays have excellent sensitivity for diagnosis o

236 erinatally-exposed infants tested by HCV-RNA RT-PCR at age 2-6 months.

237 We hypothesized that modern HCV-RNA RT-PCR platforms would adequately detect infected infant

238 d, rat IgG prevalence ranged 2%-70% and SEOV RT-PCR positivity ranged 0%-70%.

239 RT-PCR assay and next-generation sequencing RT-PCR, 419 (46.3%) tested positive for SARS-CoV-2.

240 ion proteins, siRNA-mediated gene silencing, RT-PCR, and knockout mice, we investigated whether IQGAP

241 mune cell infiltrate composition, by in situ RT-PCR and quantitative real-time PCR of laser microdiss

242 An adipogenesis-specific RT-PCR array showed that male MFR adipocytes were progra

243 confirmed dengue (VCD) by serotype-specific RT-PCR.

244 llance to detect dengue by serotype-specific RT-PCR.

245 detect target amplicons produced by standard RT-PCR or isothermal recombinase polymerase amplificatio

246 diagnosed by a positive nasopharyngeal swab RT-PCR for SARS-CoV-2 infection.

247 cific B cells and a multiplexed and targeted RT-PCR approach to measure expression levels of 96 genes

248 .03) and EV patients (65.4%; P < 0.001) than RT-PCR-negative patients (48.5%).

249 The RT-PCR did not detect viral RNA in the wall of small int

250 s or had unknown or excess delay between the RT-PCR test and CT.

251 iagnostic hypothesis, strongly linked to the RT-PCR results for the "typical," "atypical," and "negat

252 analyzed by two radiologists blinded to the RT-PCR results.

253 or are seeking to implement high-throughput RT-PCR testing to stem the SARS-CoV-2 pandemic.

254 35 syndromic patients, screened by real time RT-PCR, 56.4% of the cases were attributed to CHIKV, 29.

255 improving detection of virus using real-time RT-PCR across a range of viral titers (100-1,000,000 vir

256 A-sequencing and stem cell pathway real-time RT-PCR analysis revealed profound reductions in WNT1 exp

257 a total of 905 patients tested by real-time RT-PCR assay and next-generation sequencing RT-PCR, 419

258 Control and Prevention SARS-CoV-2 real-time RT-PCR assay, with 95% positive predictive agreement and

259 S-CoV-2 and Hologic Panther Fusion real-time RT-PCR assays for detection of viral RNA in stool specim

260 analyzed using two EV-D68-specific real-time RT-PCR assays, Sanger sequencing and metatranscriptomic

261 were tested with the CDC 2019 nCoV real-time RT-PCR diagnostic panel (4 February 2020 version), with

262 Real-time RT-PCR targeting the IAV M gene revealed detectable IAV

263 Real-time RT-PCR was used to detect viral RNA from a throat+nose s

264 ription polymerase chain reaction (real-time RT-PCR), and microarrays.

265 a single viral transport media for real-time RT-PCR.

266 s/mL, making it an attractive alternative to RT-PCR assays.

267 serve as a complementary diagnostic tool to RT-PCR.

268 SARS-CoV-2 Real-time Reverse Transcriptase (RT)-PCR Diagnostic Panel (modified CDC) assay, the Simpl

269 of the CDC Real-time Reverse Transcriptase (RT)-PCR Diagnostic Panel and two commercial automated fo

270 samples by Real-time Reverse Transcriptase (RT)-PCR or other molecular methods.

271 in the tails of sheep through transcriptome, RT-PCR, qPCR, and Western blot analyses.

272 Here, we describe a new triplex RT-PCR assay to detect tox and distinguish C. diphtheria

273 All patients underwent RT-PCR and chest CT.

274 ourteen viruses, including SARS-CoV-2, using RT-PCR.

275 d assay sensitivity was 100%/98.5%/89% using RT-PCR Ct thresholds of 30, 35 and none.

276 ase 5, GRK5; and beta-arrestin, Arrb2) using RT-PCR, qPCR, and western blot analyses.

277 n 1 (FLG-1) expression was analyzed by using RT-PCR and immunostaining in skin biopsy specimens and p

278 (IL-8 and TNF-alpha) were measured by using RT-PCR in freshly isolated cells and in response to 10(-

279 ostic performance of CT was calculated using RT-PCR as the reference standard.

280 ood samples spiked with 50 LNCaP cells using RT-PCR and Sanger sequencing.

281 Stool sample examination was performed using RT-PCR.

282 patients who were positive for COVID-19 via RT-PCR who presented with normal CT scans, correctly ide

283 Among 1,926 patients, 345 (17.9%) were RT-PCR positive for EV and 172 (8.9%) were positive for

284 With RT-PCR test results as the reference standard, the AI sy

285 symptoms tested positive for SARS-CoV-2 with RT-PCR; this yield increased in conjunction with communi

286 The POC tests are in complete agreement with RT-PCR controls.

287 C of 0.87 (95% CI: 0.84, 0.89) compared with RT-PCR and 0.87 (95% CI: 0.85, 0.89) compared with the c

288 "atypical," and "negative" and compared with RT-PCR for 460 patients.

289 When compared with RT-PCR, low-dose submillisievert chest CT demonstrated e

290 for identifying COVID-19 cases compared with RT-PCR.

291 diagnosis, either laboratory confirmed with RT-PCR, suspected with symptoms and contacts, or radiolo

292 Immunocompetent patients diagnosed with RT-PCR-confirmed severe acute respiratory syndrome coron

293 I: 18.7, 35.9) for a COVID-19 diagnosis with RT-PCR and an odds ratio of 30.6 (95% CI: 21.1, 44.4) wi

294 rs (2013-2018) and tested for influenza with RT-PCR; results were unavailable for clinical decision m

295 The proportion of patients with RT-PCR-confirmed COVID-19 in the chest CT categories typ

296 b samples and obtain consistent results with RT-PCR assay.

297 ture or observe SARS-CoV-2 in specimens with RT-PCR positivity.

298 that, in a high-risk pregnancy cohort, ZIKV RT-PCR positivity in the neonate at birth is strongly as

299 the 574 women evaluated, 44 (7.7%) were ZIKV RT-PCR positive during pregnancy.

300 the 409 neonates tested, 19 (4.6%) were ZIKV RT-PCR positive in the first 10 days of life.