ライフサイエンスコーパス: SAM domain

1 clusters was impaired by the deletion of the SAM domain.

2 left that they form together with the stable SAM domain.

3 e UBC-9 through its evolutionarily conserved SAM domain.

4 egion, a PEST domain, and a carboxy-terminal SAM domain.

5 ort the X-ray crystal structure of the Tric1 SAM domain.

6 , or Zn(2+)-dependent multimerization of the SAM domain.

7 6 N-terminal tyrosines (3Y) dependent on the SAM domain.

8 n(2+)-dependent polymerization of the Shank2-SAM domain.

9 lays a substantial drop after removal of the SAM domain.

10 cient PRE recruitment requires an intact Scm SAM domain.

11 h a reaction that requires an intact radical SAM domain.

12 eract with both Yan and Pnt-P2 through their SAM domains.

13 zosaccharomyces pombe and interact via their SAM domains.

14 Yan and Mae interact through their SAM domains.

15 egion of interaction to their respective SPM/SAM domains.

16 dules when searching for binding partners of SAM domains.

17 ng a new mode of oligomeric organization for SAM domains.

18 tion of the linker connecting its kinase and SAM domains.

19 o study the behavior of phosphorylated EphA2 SAM domains.

20 coiled-coil domains followed by three tandem SAM domains.

21 ity of its intraluminal sterile alpha-motif (SAM) domain.

22 eucine 920 in the EphA4 sterile alpha motif (SAM) domain.

23 the alpha9-helix in the sterile alpha motif (SAM) domain.

24 idues in the C-terminal Sterile Alpha Motif (SAM) domain.

25 iated by the N-terminal sterile alpha motif (SAM) domain.

26 elta and eta, contain a sterile alpha motif (SAM) domain.

27 tumor domain (MBT) and sterile alpha motif (SAM) domains.

28 work revealed that the sterile alpha motif (SAM) domain 14 (Samd14) gene increases the regenerative

29 racted from the substrate by the two radical SAM domains, (2) the second tyrosine-derived product, (3

30 It contains a sterile-alpha motif (SAM) domain, 3 phosphotyrosine motifs, a proline-rich re

31 ructure reveals an unusual makeup in which a SAM domain, a common protein-protein interaction module,

32 SOP-2 contains a SAM domain, a self-associating protein domain found in o

33 L920F mutant are affected differently by the SAM domain and are differentially regulated by ephrin li

34 In this context, dNTP triphosphohydrolase SAM domain and HD domain-containing protein 1 (SAMHD1) h

35 SAM domain and HD domain-containing protein 1 (SAMHD1),

36 those required for viral cDNA synthesis by a SAM domain and HD domain-containing protein 1 (SAMHD1)-i

37 ot part of the hydrophobic core of the EphB2-SAM domain and is conserved for functional reasons.

38 ild-type EphA4 and a mutant lacking both the SAM domain and PDZ binding motif were constitutively tyr

39 AlphaFold2 structure prediction of both the SAM domain and Tric1 support a cyclic pentameric or hexa

40 has attributes that are distinct from other SAM domains and underlie SAMD14 function as a regulator

41 racteristic RNA-binding sterile-alpha motif (SAM) domain and a conserved but uncharacterized N-termin

42 rising the polymerizing sterile alpha motif (SAM) domain and its adjacent catalytic domain.

43 ntifies the domain as a sterile alpha-motif (SAM) domain and shows a propensity to oligomerize.

44 in part to the nucleotidase activity of the SAM-domain and HD-domain containing protein (SAMHD1), wh

45 of 871 amino acids carrying an SH3 domain, a SAM domain, and a PH domain.

46 id substitutions in the sterile alpha motif (SAM) domain, and are predicted to affect protein--protei

47 The HIV-1 restriction factor SAM domain- and HD domain-containing protein 1 (SAMHD1)

48 l expression of the HIV-1 restriction factor SAM domain- and HD domain-containing protein 1 (SAMHD1)

49 SAMHD1 (SAM domain- and HD domain-containing protein 1) is a dGT

50 a four-helix bundle (H2-H5), resembling the SAM domain, appended with two additional helices (H0-H1)

51 SAM domains are known to form homo- and hetero-oligomers

52 ein interaction studies demonstrate that the SAM domains are necessary and sufficient to mediate SARM

53 lex interactions between the coiled-coil and SAM domains are thought to create liprin scaffolds, but

54 Although SAM domains are very commonly found in eukaryotic protei

55 Sterile alpha motif (Sam) domains are protein interaction modules that are im

56 ce, reduces self-association of the isolated SAM domain as well as high molecular mass complex format

57 ty depends on the integrity of the protein's SAM domains, as well as on the enzymatic conversion of N

58 enable its activation by dimerization of its SAM domains at the collision interface.

59 tingly, deletion of the sterile alpha motif (SAM) domain at the N terminus dramatically reduced the u

60 unique in possessing a sterile alpha motif (SAM domain) at their C-terminal ends.

61 The n-NafY SAM domain binds apo-NifDK.

62 We show that not only the SAM domain but also the N-terminal tail engages in the D

63 he resulting transformants indicate that the SAM domain but not the RA domain is essential for the fu

64 ive-helix-bundle architecture of traditional SAM domains, but has additional short helices at N and C

65 sed on MD results, we targeted the two STIM1 SAM domains by engineering point mutations.

66 These results indicate that SAM domains can create a variety of oligomeric architect

67 providing the first clear demonstration that SAM domains can polymerize.

68 SAM domains can self-associate to form higher-order stru

69 In this study, we have identified SAM domain-carrying non-receptor tyrosine kinase, activa

70 suggest that the L920F mutation alters EphA4 SAM domain conformation, leading to the formation of Eph

71 SAM domain containing proteins have been shown to requir

72 Finally, the human HemW orthologue radical SAM domain-containing 1 (RSAD1) stably bound heme.

73 Viperin (also known as radical SAM domain-containing 2 (RSAD2)) is an interferon-induci

74 Here, we identify and characterize SAMD1 (SAM domain-containing protein 1) as an unmethylated CGI-

75 he crystal structure of the NTD of the human SAM domain-containing protein 4A (SAMD4A, a.k.a. Smaug1)

76 Previously, we demonstrated that a SAM domain-containing protein, SAMD14, promotes SCF/prot

77 Byr2 and Ste4 are two SAM domain-containing proteins in the mating pheromone r

78 acterize the Drosophila sterile alpha-motif (SAM) domain-containing protein Caskin, which shares homo

79 Furthermore, the H5 alpha helix within the SAM domain contributed to self-association.

80 ur results also suggest a mechanism by which SAM domains could mediate the spreading of transcription

81 n, we generated SLP-76 knockin mice with the SAM domain deleted.

82 Our results indicate that SAM domain deletion (EphA2DeltaS-GFP) increases oligomer

83 These results suggest that SAM domain deletion induced constitutive activation of E

84 The SAM domain deletion mutant, EphA2DeltaS-GFP, also underw

85 tion and abrogated by the deletion of SLP-76 SAM domain (DeltaSAM) or mutation of Tyr-113, Tyr-128, a

86 tations on Mae that specifically disrupt its SAM domain-dependent interactions with Yan disable the d

87 gated the potential role of zinc in DGKdelta SAM domain (DGKdeltaSAM) assembly.

88 Previous crystal structures of an EphA4-SAM domain dimer and a possible EphB2-SAM oligomer both

89 ins green fluroscent protein (GFP) or EphB2 (SAM domain) displayed markedly increased growth rates wi

90 horylation levels upon deletion of the EphA2 SAM domain (EphA2DeltaS) in DU145 and PC3 prostate cance

91 been taken over by a different branch of the SAM domain family during the evolution of nematodes.

92 n EphB2-SAM domain that has the same overall SAM domain fold yet has no substantial intermolecular co

93 s a three-dimensional docking surface on its SAM domain for the MAP kinase, Rolled.

94 Here we show that the TEL-SAM domain forms a helical, head-to-tail polymeric struc

95 The SAM domain forms a novel antiparallel double helix, posi

96 The crystal structure of the SAM domain from an Eph receptor tyrosine kinase, EphB2,

97 n from the lipid phosphatase Ship2 binds the Sam domain from the EphA2 receptor to negatively regulat

98 nd its possible mode of interaction with the Sam domain from the EphA2 receptor.

99 ubstrate receptor, DCAF1, and N-terminal and SAM domains from mandrill SAMHD1.

100 To elucidate the molecular determinants of SAM domain function in SAMD14, we substituted its SAM do

101 We determined that the amino-terminal SAM domain functions as an autoinhibitory domain of intr

102 Thus, the SAMD14 SAM domain has attributes that are distinct from other S

103 We showed previously that the SAM domain has two binding regions that mediate dimer an

104 Hundreds of sterile alpha-motif (SAM) domains have predicted structural similarities and

105 fection in macrophages involves the cellular SAM domain HD domain-containing protein 1 (SAMHD1).

106 dissociation process of the EphA2-SHIP2 SAM-SAM domain heterodimer complex using unrestrained all-at

107 at of dNumb developmental regulators and two SAM domains homologous to those in the C-terminal tail o

108 tantly, aberrant C-terminal extension of the SAM domain in bpk mutant Bicc1 phenocopied these defects

109 escence study to investigate the role of the SAM domain in EphA2 function.

110 Aberrant localization of the Scm-SAM domain in long contiguous regions on polytene chromo

111 identified for the first time a role for the SAM domain in mediating SLP-76 self-association for T-ce

112 The presence of a SAM domain in NafY was unexpected and could not be infer

113 structural basis for the versatility of the SAM domain in protein and RNA-recognition.

114 SAM domain of ZAK supports a key role of the SAM domain in regulating kinase activity on and off the

115 ve been extensively studied, the role of the SAM domain in SLP-76 function is not known.

116 tirely different from that of the equivalent SAM domain in SRF and MCM1, accounting for the absence o

117 ins that become ordered upon binding, the EF-SAM domain in the stromal interaction molecule (STIM) 1

118 binding by an adaptor and suggest a role for SAM domains in clathrin-mediated endocytosis.

119 However, the functions of SAM domains in Eph receptors remain elusive.

120 SAM domains in some systems have been shown to self-asso

121 The presence of PDZ and SAM domains in the KS5 protein suggests that it may act

122 binding, the solution structure of the Vts1 SAM domain, in the presence of a specific target RNA, ha

123 60L mutation while the R926C mutation in the SAM domain increases S906 phosphorylation.

124 In contrast, a deletion of the SAM domain induces a complex hindlimb defect associated

125 on these results, we conclude that the EphA2 SAM domain inhibits kinase activity by reducing receptor

126 A heterotypic Sam-Sam domain interaction is mediating this process.

127 via a heterotypic sterile alpha motif (SAM)-SAM domain interaction, leading to regulation of EphA2 i

128 The SAM domain interacts with L3MBTL3, but it can also homop

129 4 trimers that assemble through two aberrant SAM domain interfaces.

130 Delivery of the SAM domain into macrophages via the TAT cell-penetrating

131 es block the stacking of helical polymers of SAM domains into sheets through side-by-side contacts, w

132 ammalian cells, we found that the N-terminal SAM domain is important for self-association and kinase

133 Altogether, our data demonstrated that the SAM domain is indispensable for optimal SLP-76 signaling

134 hening of Mtrm::Polo binding mediated by the SAM domain is necessary to prevent meiotic catastrophe a

135 Although Mtrm's C-terminal SAM domain is not required to rescue the chromosome segr

136 Chimeric constructs in which the SOP-2 SAM domain is replaced with that derived from fruit fly

137 G proteins, but not those in which the SOP-2 SAM domain is replaced with the SAM domains of non-PcG p

138 1 are associated with its HD domain, but the SAM domain is required for maximal activity and nucleic

139 The sterile alpha motif (SAM) domain is a protein interaction module that is pres

140 The sterile alpha motif (SAM) domain is a protein module found in many diverse si

141 modulated by their evolutionarily conserved SAM domain, is essential to their physiological repressi

142 ires the Scm C-terminal sterile alpha motif (SAM) domain, is crucial for the efficient sumoylation of

143 in vivo, we show that a sterile alpha-motif (SAM) domain located at the C terminus of Mtrm increases

144 loses Ca(2+) from EF hand, its intraluminal SAM domain may change conformation, and via glycosylatio

145 induce these phenotypes, suggesting that the SAM domain may negatively regulate some aspects of EphA4

146 rmined that the oligomerization of the Tric1 SAM domain may play a role in protein function whereby m

147 daptor protein that interacts with Lar via a SAM domain-mediated interaction.

148 SAM domain-mediated polymerization of EN leads to consti

149 Introduction of SAM domain missense mutations that restrict Yan to a mon

150 n of messenger RNA encoding the Elp3 radical SAM domain mutant, but not the HAT domain mutant, into M

151 Here, we have investigated how these SAM domain mutations affect EPHA2 activity.

152 We showed that the SAM domain mutations dramatically destabilized the EPHA2

153 Coexpression of combinations of SAM domain mutations that permit the formation of Yan di

154 Tu et al. showed that the isolated SAM domain of Byr2 binds a fragment of Ste4 that contain

155 unction alleles indicate that the N-terminal SAM domain of Ckn mediates its interaction with Lar.

156 The SAM domain of DGKdelta1 forms helical polymers that are

157 Mutations in the SAM domain of Shank3 result in altered synaptic function

158 hermal titration calorimetry) studies on the Sam domain of Ship2 revealing its three-dimensional stru

159 that glycosylation sites in the ER-resident SAM domain of STIM1 are essential for initiation of CIF

160 ts fuse a potent oligomerization module, the SAM domain of TEL, to a variety of tyrosine kinases or t

161 Taken together, our studies suggest that the SAM domain of the EPHA2 protein plays critical roles in

162 Ship2 that retains binding affinity for the Sam domain of the EphA2 receptor.

163 earing loss, due to mutations disrupting the SAM domain of the protein kinase ZAK.

164 as well as a known pathogenic variant of the SAM domain of ZAK supports a key role of the SAM domain

165 ydG showed that the highly conserved radical-SAM domains of both HydE and HydG and the GTPase domain

166 ch the SOP-2 SAM domain is replaced with the SAM domains of non-PcG proteins, confer appropriate in v

167 ted with SARM1 in vitro and in vivo and that SAM domains of SARM1 were necessary for ULK1-SARM1 compl

168 Here, we show that the individual SAM domains of Ste4 and Byr2 are monomeric at low concen

169 The lumenal EF-hand and SAM domains of STIM1 are believed to initiate oligomeriz

170 these data reveal that interactions between SAM domains of STIM1 monomers are critical for multimeri

171 Here we examine the interaction between the SAM domains of the polycomb group proteins polyhomeotic

172 ions in the cytoplasmic sterile-alpha-motif (SAM) domain of human EPHA2 on chromosome 1p36 have been

173 The sterile alpha-motif (SAM) domain of Mst50 was essential for its interaction w

174 ted by mutations in the sterile alpha-motif (SAM) domain of p63 that are associated with ankyloblepha

175 zinc to the C-terminal sterile-alpha-motif (SAM) domain of Shank3.

176 that the N-terminal sterile alpha motif (or SAM) domain of SMSr drives self-assembly of the protein

177 The sterile alpha motif (SAM) domain of the ephrin receptor tyrosine kinase, EphA

178 the SAMHD1 N-terminal tail and the adjacent SAM domain or the C-terminal tail proceeding the HD doma

179 We find that the EF-SAM domain partially unfolds and dimerizes cooperatively

180 A third system pairs a radical SAM domain peptide maturase with selenocysteine-containi

181 roles in protein-protein interactions, some SAM domains play crucial roles in RNA binding.

182 This work reveals a novel SAM domain polymerization mode, illustrates how supramol

183 we substituted its SAM domain with distinct SAM domains predicted to be structurally similar.

184 regulated enhancer controls expression of an SAM domain protein that confers survival in anemia.

185 (Samd14-Enh) encoding a sterile alpha motif (SAM) domain protein.

186 ure, the N-terminal peptide arm of the EphB2-SAM domain protrudes out from the core of the molecule,

187 Moreover, a mutant DGKdelta containing a SAM domain refractory to zinc binding diminishes the for

188 was predicted to disturb the topology of the SAM domain region that is essential for protein-protein

189 cted structure modeling and then mutated the SAM domain residues which in this model were predicted t

190 nt microscale thermophoresis of the isolated SAM domain (residues 1-78) revealed evidence of dimers a

191 The Vts1 SAM domain retains the "core" five-helix-bundle architec

192 A K78E missense mutation within the SAM domain, revealed a genetic interaction between ubc2

193 merizing TNKS and TNKS2 sterile alpha motif (SAM) domains, revealing versatile head-to-tail interacti

194 8 to Ala-8 mutation and found that the EphB2-SAM domain structure and stability were only slightly al

195 leucine zipper (Ste4-LZ) domain as well as a SAM domain, suggesting that Byr2-SAM and Ste4-SAM may fo

196 the catalytic [Fe4S4] cluster in the radical SAM domain, surprisingly, does not abolish the inhibitor

197 solved a new crystal form of the human EphB2-SAM domain that has the same overall SAM domain fold yet

198 nd genetic analysis that it is primarily the SAM domain that interacts specifically with the appropri

199 a structural model of the Ca(2+)-unbound EF-SAM domain that is consistent with a wide range of evide

200 ne-rich region that binds to cortactin and a SAM domain that mediates multimerization.

201 nd that the interface maps to regions of the SAM domains that are known to be important for the forma

202 N-terminal autoinhibitory ARM domain, tandem SAM domains that mediate multimerization, and a C-termin

203 motif) domain is a member of a new class of SAM domains that specifically bind RNA.

204 a small protein with a sterile alpha motif (SAM) domain that can physically interact with the scaffo

205 us of Shank3 contains a sterile alpha motif (SAM) domain that is essential for its postsynaptic local

206 ere, we examine the response of the STIM1 EF-SAM domain to changes in Ca(2+) concentration using math

207 We identified the ability of the SAM domain to form a helical superstructure with six mon

208 that full-length Yan self associates via its SAM domain to form higher-order complexes in living cell

209 polymerizes through its sterile alpha motif (SAM) domain to assemble large protein complexes.

210 Moreover, mutation of a conserved SAM domain tyrosine to phenylalanine (Y928F) enhanced th

211 The conserved SAM domain was required for SAMD14 to increase colony-fo

212 l activity of viperin depends on its radical SAM domain, which contains conserved motifs to coordinat

213 The BAR protein contains a SAM domain, which is required for its interactions with

214 145, "3Y") as well as a sterile alpha motif (SAM) domain whose function is unclear.

215 omain function in SAMD14, we substituted its SAM domain with distinct SAM domains predicted to be str

216 93F, Y587E/Y593E), kinase domain (Y734F), or SAM domain (Y929F) inhibited ephrin-A1-induced vascular

217 is trimeric and, when included with the Ste4-SAM domain, yields a 3:1 Ste4-LZ-SAM:Byr2-SAM complex wi