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1                                              SL RNA genes are individually transcribed from the only
2                                              SL-RNA degraded more rapidly during treatment than kDNA,
3                                              SL-RNA quantitative polymerase chain reaction emerges as
4 t decreased in vitro eEF1A binding to the 3' SL RNA also decreased viral minus-strand RNA synthesis i
5 ed the efficiency of eEF1A binding to the 3' SL RNA increased minus-strand RNA synthesis in transfect
6  The data suggest that interaction of the 3' SL RNA with eEF1A at three sites and a unique metastable
7 n reduced the binding activity of the WNV 3' SL RNA for EF-1 alpha by approximately 60%.
8 he interaction between EF-1 alpha and WNV 3' SL RNA was calculated to be 1.1 x 10(-9) M.
9 he interaction between EF-1 alpha and WNV 3' SL RNA was specific.
10  extracts and in vitro-synthesized WNV (-)3' SL RNA as the probe.
11 cted in BHK cell extracts with the WNV (-)3' SL RNA were found in both C3H/RV and C3H/He cell extract
12 by UV-induced cross-linking to the WNV (-)3' SL RNA.
13 WNV 3' minus-strand stem-loop RNA [WNV (-)3' SL RNA] confirmed the existence of a terminal secondary
14  EF-1 alpha binds to each molecule of WNV 3' SL RNA.
15  binding site on the main stem of the WNV 3' SL RNA.
16 d that specifically interact with the WNV 3' SL RNA.
17  phosphatase inhibited its binding to WNV 3' SL RNA.
18 unoprecipitated both the RV 5'(+)SL and 3'(+)SL RNA-protein complexes.
19 Autoantigens binding the RV 5'(+)SL and 3'(+)SL RNAs differed in molecular mass, specificities for re
20 eraction between TIAR RRM2 and the WNV 3'(-) SL RNA was 1.5 x 10(-8), while that for TIA-1 RRM2 was 1
21 lasmic extracts incubated with the WNV 3'(-) SL RNA was immunoprecipitated by anti-TIAR antibody.
22           The binding site for the WNV 3'(-) SL RNA was mapped to RRM2 on both TIAR and TIA-1.
23            TIA-1 also binds to the WNV 3'(-) SL RNA.
24 e West Nile virus minus-stand RNA [WNV 3'(-) SL RNA].
25 action between TIAR/TIA-1 and the viral 3'(-)SL RNA is not required for initial low-level symmetric R
26 ) which was able to dissociate LARP6 from 5' SL RNA in vitro and to inactivate the binding of endogen
27 lular RNA-binding protein binds to the RV 5' SL RNA with sufficient specificity for the binding to oc
28 stability of complex between La domain and 5'SL RNA and RRM domain does not make extensive contacts w
29 face enables the La domain to discriminate 5'SL RNA from homopolymeric or purely helical hairpin RNAs
30 The major site of crosslinking of LARP6 to 5'SL RNA was mapped to this motif, as well.
31 ds two domains to form stable complex with 5'SL RNA, the La domain and the juxtaposed RNA recognition
32 main does not make extensive contacts with 5'SL RNA.
33 tigen was found to interact with the RV 5'(+)SL RNA as determined by immunological techniques and bin
34 cellular RNA, with significant homology to 7 SL RNA is associated with Puralpha.
35         We report the characterization of an SL RNA processing complex with TbMTr1 and the SLA1 H/ACA
36                                   To analyze SL RNA genesis, we mutated SL RNA intron structures and
37 ere indispensable for parasite viability and SL RNA gene transcription.
38     We conclude that TbCgm1 specifically cap SL RNA, and cap 0 is a prerequisite for subsequent methy
39 ccumulating species in SNIP knockdown cells, SL RNA 3'-end formation is a multistep process in which
40 rypanosomes, we have begun by characterizing SL RNA gene expression in the model trypanosome Leptomon
41 then verified by interrogating corresponding SL RNA genes for sequence motifs expected in bona fide S
42 hom human African trypanosomiasis was cured, SL RNA detection had specificities of 98.4%-100%, while
43 TAP-null lines are viable but have decreased SL RNA processing efficiency in cap methylation, 3'-end
44                          In dinoflagellates, SL RNAs are unusually short at 50-60 nt, with a conserve
45 ith an m(2,2,7)G-cap and a common downstream SL RNA sequence.
46                                       During SL RNA transcription, accurate initiation is determined
47                  Accumulation of 3'-extended SL RNA substrate indicates a delay in processing and sug
48 es for sequence motifs expected in bona fide SL RNA molecules.
49 ription factor IIB (tTFIIB) is essential for SL RNA gene transcription and cell viability, but has a
50 ence element and is absolutely essential for SL RNA gene transcription in vitro.
51  associates with tSNAPc and is essential for SL RNA gene transcription.
52 nosoma cruzi (CL Brener) genome projects for SL RNA repeat sequences in order to assess their homogen
53 onexosomal, 3'-->5' exonuclease required for SL RNA 3'-end formation in Trypanosoma brucei.
54 splicing, in which a capped SL, derived from SL RNA, is spliced onto the 5' end of each mRNA.
55                 The spliced leader RNA gene (SL RNA) repeat is present in large multicopy arrays and
56 d and are predicted to yield a heterogeneous SL RNA population.
57  predicted secondary structures of the Hydra SL RNAs show significant differences from the structures
58 A and lead to accumulation of hypomethylated SL RNA.
59 ption initiates at the +1 of the interrupted SL RNA gene and proceeds into the 5' UTR and open readin
60 is common to the 3' end of all kinetoplastid SL RNA genes, and that more than six T's are required fo
61 ma brucei, the small nuclear spliced leader (SL) RNA and the large rRNAs are key molecules for mRNA m
62 m7G cap is restricted to the spliced leader (SL) RNA and the precursors of U2, U3, and U4 snRNAs.
63 tiation complex (PIC) at the spliced leader (SL) RNA gene (SLRNA) promoter.
64 rms are generated in which a spliced leader (SL) RNA gene is fused with a sequence tag and driven by
65 egrates exclusively into the spliced leader (SL) RNA genes.
66 port the first data that the spliced leader (SL) RNA is a more specific marker for cure of human Afri
67 atids, trans splicing of the spliced leader (SL) RNA is a required step in the maturation of all nucl
68                              Spliced leader (SL) RNA trans splicing adds a trimethylguanosine (TMG) c
69                              Spliced leader (SL) RNA trans-splicing contributes the 5' termini to mRN
70 ase II-mediated synthesis of spliced leader (SL) RNA, a trans splicing substrate and key molecule in
71 lies on the synthesis of the spliced leader (SL) RNA.
72 zi, and Leishmania spp., the spliced-leader (SL) RNA is a key molecule in gene expression donating it
73 from a short transcript, the spliced-leader (SL) RNA.
74                      The two spliced leader (SL) RNAs (SL-A and -B) contain splice donor dinucleotide
75 f cap 4 formation on the 140-nucleotide-long SL RNA.
76 le virus minus-strand 3' terminal stem loop (SL) RNA was previously shown to bind specifically to cel
77 eins associated with the RV 5'(+) stem-loop (SL) RNA are recognized by serum with Ro reactivity.
78 e cell protein eEF1A on the 3'(+) stem-loop (SL) RNA of West Nile virus (WNV) (3).
79  a class of temperature-sensitive stem-loop (SL) RNAs called RNA thermometers (RNATs).
80 on events leading to the formation of mature SL RNA.
81        To analyze SL RNA genesis, we mutated SL RNA intron structures and sequence elements: stem-loo
82 6 nucleotides result in the appearance of no SL RNA, consistent with the in vitro studies by others s
83 mediator is a basal factor for small nuclear SL RNA gene transcription in trypanosomes and that the b
84 ng domain, human 3'hExo, and a 26-nucleotide SL RNA.
85 y, SPF27 silencing caused an accumulation of SL RNA with a hypomethylated cap that closely resembled
86    Towards understanding the coordination of SL RNA and mRNA expression in trypanosomes, we have begu
87  of one- to two- nucleotide 3' extensions of SL RNA, U2 and U4 snRNAs, a five-nucleotide extension of
88 organisms crucially depend on high levels of SL RNA synthesis.
89 smic machinery responsible for processing of SL RNA.
90 th alpha-amanitin sensitivity reminiscent of SL RNA synthesis and is dependent on the SL RNA promoter
91 ent and nucleoplasmic Sm-dependent stages of SL RNA maturation combined with reduced rates of transla
92  Here, we analyzed the spatial expression of SL RNAs in the planarian flatworm Schmidtea mediterranea
93 4 modification, indicating that SNIP acts on SL RNA after cytosolic trafficking.
94 and TbCMT1 did not affect parasite growth or SL RNA capping.
95 A of uniform sequence (the spliced leader or SL RNA) has allowed us to characterize the RNAs with whi
96                            Since the primary SL RNA transcript was not the accumulating species in SN
97                                  As putative SL RNA gene transcription factors, a partially character
98              The role of spliced leader RNA (SL RNA) in trans-splicing in Caenorhabditis elegans has
99 gm1 transfers the GMP to spliced leader RNA (SL RNA) via a covalent enzyme-GMP intermediate, and meth
100 g-specific approximately 100-nucleotide RNA (SL RNA) that bears striking similarities to the cis-spli
101 netoplast DNA (kDNA) and spliced-leader RNA (SL-RNA) in vitro, in vivo, and in a VL patient cohort.
102 sfer of a short leader sequence from a small SL RNA to the 5' end of a subset of pre-mRNAs.
103 rified and characterized a sequence-specific SL RNA promoter-binding complex, tSNAP(c), from the path
104  localized to the nucleoplasm, and substrate SL RNA derived from SNIP knockdown cells showed wild-typ
105 '-O-ribose methylation at cap 1 on substrate SL RNA and U1 small nuclear RNA.
106  TbMTr2-/-/TbMTr3-/- lines yielded substrate SL RNA and mRNA with cap 1.
107                         Using an exon-tagged SL RNA gene, we examined the phenotypes produced by a se
108   Only in the tissue of interest, the tagged SL RNA gene is transcribed and then trans-spliced onto m
109 with similar efficiencies to the 3'-terminal SL RNAs of four divergent flaviviruses, including a tick
110  could be detected on prematurely terminated SL RNA transcripts of 56 nucleotides in length and longe
111 ta from our proof-of-concept study show that SL RNA detection has high potential as a test of cure.
112   The in vivo and in vitro data suggest that SL RNA transcription termination is staggered in the T t
113                                          The SL RNA cap structure in Trypanosoma brucei is unique amo
114                                          The SL RNA gene (SLRNA) promoter recruits RNA polymerase II
115                                          The SL RNA gene in Leptomonas seymouri is a member of the sm
116                                          The SL RNA gene promoter has been shown by in vitro and in v
117                                          The SL RNA genes are expressed from the only defined RNA pol
118                                          The SL RNA in Leishmania tarentolae is a 96-nt precursor tra
119                                          The SL RNA is a small nuclear RNA and a trans splicing subst
120                                          The SL RNA is present in the cell in the form of a ribonucle
121                                          The SL RNA is transcribed with an oligo-U 3' extension that
122 f vertebrate small nuclear RNA genes and the SL RNA homologue in Ascaris.
123  by eIF4E/G interaction with the cap and the SL RNA, although the SL does not increase the affinity o
124 f the most abundant crosslinks formed by the SL RNA allowed us previously to identify the spliced lea
125 ear RNAs U1, U2, U4 and U5; for example, the SL RNA functions only if it is assembled into an Sm smal
126 he transcription termination element for the SL RNA gene.
127                                 However, the SL RNA is required in stoichiometric amounts for trans-s
128 bsequent modifications characteristic of the SL RNA cap 4 were added successively in a 5' to 3' direc
129                         m(7)G capping of the SL RNA could be detected on prematurely terminated SL RN
130                            Expression of the SL RNA gene requires sequence elements at bp -60 to -70
131 w here that the 5' splice site region of the SL RNA is also crosslinked in vivo to a second small RNA
132                            Expression of the SL RNA is initiated at the only known RNA polymerase II
133 e capped spliced leader (SL) sequence of the SL RNA onto the 5' end of all mRNAs.
134 n, that was caused by hypomethylation of the SL RNA's unique cap4.
135  of SL RNA synthesis and is dependent on the SL RNA promoter.
136  processing of multiple modifications on the SL RNA substrate.
137 sm for generating the cap 4 structure on the SL RNA.
138 ence, which is derived from a small RNA, the SL RNA, to all mRNA precursors.
139 ystem, we demonstrate in this study that the SL RNA is transcribed by RNA polymerase II.
140 P(c)/TFIIA complex binds specifically to the SL RNA gene promoter upstream sequence element and is ab
141 ipate in recruiting RNA polymerase II to the SL RNA gene promoter.
142  13, 12, and 8 kDa were co-selected with the SL RNA from Leptomonas collosoma, representing the SL RN
143 initiation complex that assembles within the SL RNA gene promoter.
144 s robust transcriptional activity within the SL RNA gene.
145                                  Both of the SL RNAs are bound by antibody against trimethylguanosine
146 1 and SL2, S. mediterranea expresses a third SL RNA described here as SL3.
147 lex enables efficient translation of the TMG-SL RNAs in diverse in vitro translation systems.
148  In SL trans-splicing, SL-donor transcripts (SL RNAs) contain two functional domains: an exon that pr
149 t and fourth (m(7)Gpppm(6,6)AmpAmpCmpm(3)Ump-SL RNA) conveyed via trans-splicing of a universal splic
150 rference increased the abundance of uncapped SL RNA and lead to accumulation of hypomethylated SL RNA
151 s, including Caenorhabditis elegans, utilize SL RNA in their mRNA maturation programs.
152                    We describe mutants whose SL RNAs end in the T tract or appear to lack efficient t
153  the present study, the sites on the WNV3'(-)SL RNA required for efficient in vitro T-cell intracellu
154 located in two internal loops of the WNV3'(-)SL RNA structure.
155 ile virus 3' minus-strand stem-loop [WNV3'(-)SL] RNA (37) and colocalized with flavivirus replication

 
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