ライフサイエンスコーパス: SR protein

1 ment) serine/arginine-rich splicing factors (SR proteins).

2 and requires collaboration of more than one SR protein.

3 e recognition, binding, and transport of the SR protein.

4 man SRPK1 bound to a peptide derived from an SR protein.

5 of the RS domain of ASF/SF2, a prototypical SR protein.

6 xons contain less putative binding sites for SR proteins.

7 ulates AS by modulating the levels of active SR proteins.

8 s cellular levels of phosphorylated forms of SR proteins.

9 phases of the processive phosphorylation of SR proteins.

10 n Casq2 but no significant decrease in other SR proteins.

11 SR protein kinases for it compared to other SR proteins.

12 is via SRPK2, a key regulator of RNA-binding SR proteins.

13 redict putative ESEs specific for four human SR proteins.

14 The yeast Saccharomyces cerevisiae lacks SR proteins.

15 ures of SRp38 that distinguish it from other SR proteins.

16 ng distinct recycling pathways for different SR proteins.

17 ownstream exon even in the absence of active SR proteins.

18 nuclear translocation and phosphorylation of SR proteins.

19 known functional parallels between EJCs and SR proteins.

20 NA complex with other known RNA complexes of SR proteins.

21 influences the levels and activity of other SR proteins.

22 of a set of factors that includes eIF3f, the SR protein 9G8, and the cyclin-dependent kinase 11 (CDK1

23 in the exonized segment and was promoted by SR proteins 9G8, Tra2beta and SC35.

24 The splicing regulatory SR protein, 9G8, has recently been proposed to function

25 he spliceosome requires the participation of SR proteins, a family of splicing factors rich in argini

26 psis genes that encode serine/arginine-rich (SR) proteins, a conserved family of splicing regulators

27 We show that PEs in serine-arginine-rich (SR) proteins, a family of 14 essential SFs, are differen

28 the biological function of serine-arginine (SR) proteins, a family of essential regulators of mRNA s

29 ontrol the activity of serine-arginine-rich (SR) proteins, a family of splicing co-activators, and th

30 SR proteins accumulated in patches around active nucleol

31 e the wealth of functional information about SR proteins accumulated to-date, structural knowledge ab

32 results suggest that a critical threshold of SR proteins, achieved by juxtaposition of SR protein bin

33 Although the precedent for SR proteins acting as repressors is established, this is

34 pecific effects on regulated splicing by one SR protein actually depend on a complex set of relations

35 Thus, the phosphorylation state of the SR protein adapters may underlie the selectivity of TAP-

36 Surprisingly, all other SR proteins also correlate with the minor spliceosome an

37 hnRNP and SR proteins also regulate the expression of other Drosop

38 vitro and ex vivo approaches have identified SR proteins and hnRNPs of the A/B and H subfamilies as c

39 Splicing activators and repressors, such as SR proteins and hnRNPs, modulate spliceosome assembly an

40 pacted by deregulation of expression of most SR proteins and hnRNPs.

41 ially overlaps with that of splicing-related SR proteins and in tex1 plants the ratio of certain alte

42 MALAT1 interacts with SR proteins and influences the distribution of these and

43 SR proteins and related factors play widespread roles in

44 porter system was promoted by at least seven SR proteins and repressed by hnRNPs F, H and I, supporti

45 of SRPK1 could affect the functions of host SR proteins and those of the virus transcription/replica

46 tive regulator of splicing (NRS), that binds SR proteins and U1/U11 snRNPs and functions as a pseudo-

47 constituted in fibroblasts by downregulating SR proteins and upregulating a RBFOX protein and that SR

48 exon junction complexes (EJC) together with SR proteins and was thus recruited to RNA polymerase II.

49 he interaction between serine/arginine-rich (SR) proteins and splicing components that recognize eith

50 hydroxylates multiple serine/arginine-rich (SR) proteins and SR related proteins, including U2AF65,

51 tion of a ternary complex containing ESE, an SR protein, and U1 snRNP.

52 the nucleus, differential phosphorylation of SR proteins, and alteration of splice site selection.

53 n of hnRNP proteins, the late recruitment of SR proteins, and binding of the U2 snRNP.

54 with the Env splicing enhancer, which binds SR proteins, and inactivation of the enhancer and SR pro

55 More detailed analysis showed that the SR proteins, and their RS domains in particular, are enr

56 sulin increased the amount of phosphorylated SR proteins, and this effect was counteracted by arachid

57 Mammalian SR proteins are a family of reversibly phosphorylated RN

58 SR proteins are a family of sequence-specific RNA bindin

59 SR proteins are essential splicing factors whose biologi

60 SR proteins are essential splicing factors whose functio

61 SR proteins are essential splicing regulators mostly con

62 ; yet both hypo- and hyperphosphorylation of SR proteins are known to inhibit splicing, indicating th

63 this intron are sufficient for AS, and which SR proteins are necessary for regulated AS.

64 To determine which SR proteins are responsible for AS, the splicing pattern

65 We found that shuttling and nonshuttling SR proteins are segregated in an orderly fashion during

66 to form megadalton sized complexes in which SR proteins are super-stoichiometric to EJC core factors

67 SR proteins are well known to promote exon inclusion in

68 SR proteins are well-characterized RNA binding proteins

69 Ser/Arg-rich (SR) proteins are essential nucleus-localized splicing fa

70 Serine-arginine (SR) proteins are essential splicing factors that promote

71 Serine-arginine (SR) proteins are general metazoan splicing factors that

72 nuclear export factor 1 (NXF1) by describing SR proteins as NXF1 adaptors that flag alternatively spl

73 The prototypical SR protein ASF/SF2 (human alternative splicing factor) c

74 The arginine-serine (RS)-rich domain of the SR protein ASF/SF2 is phosphorylated by SR protein kinas

75 rminal portion of the RS domain (RS1) of the SR protein ASF/SF2, a modification that promotes nuclear

76 ng enhancer, which was predicted to bind the SR protein ASF/SF2.

77 stent with this, SELEX-binding sites for the SR proteins ASF/SF2, 9G8, and SRp20 were able to stimula

78 Here we provide evidence that a prototypical SR protein, ASF/SF2, is unexpectedly required for mainte

79 strate that a heart-specific knockout of one SR protein, ASF/SF2, produces cardiomyopathy and misregu

80 The mammalian serine-arginine (SR) protein, ASF/SF2, contains multiple contiguous RS di

81 ompensatory gain in the interaction of other SR proteins at the affected exons.

82 by the loss of the pre-mRNA splicing factor SR protein B52.

83 n a mammalian pre-mRNA, a normally essential SR protein becomes dispensable when the complementarity

84 All examined SR proteins bind lincRNA exons to a much lower extent th

85 ns of RNA-binding sites showed that NXF1 and SR proteins bind mRNA targets at adjacent sites, indicat

86 In addition, SR proteins bind to the element, consistent with the pre

87 oteins, and inactivation of the enhancer and SR protein binding decreased polyadenylation efficiency.

88 of SR proteins, achieved by juxtaposition of SR protein binding sites within the NRS and Env enhancer

89 Mechanistically, SRPK2 promotes SR protein binding to U1-70K to induce splicing of lipog

90 , the N-terminus, which positively regulates SR protein binding, adopts a stable structure when the i

91 on in human exons and introns for four known SR protein-binding motifs: SF2/SAF, SC35, SRp40, and SRp

92 Deletions that positioned the SR protein-binding sites closer to the poly(A) site elim

93 Each SR protein binds diverse ESEs using strategies that are

94 insic phosphorylation bias is obligatory for SR protein biological function.

95 SM activates splicing in the manner of SR proteins but does not contain the canonical RS domain

96 Here, we report that CLK1 binds SR proteins but lacks the mechanism to release phosphory

97 increased the phosphorylation of endogenous SR proteins, but their phosphorylation state remained un

98 tein (snRNP), U2B, and serine/arginine-rich (SR) proteins], but is excluded from the coiled bodies an

99 rtant for binding and phosphorylation of the SR protein by CLK1 but not for the SRPK1-dependent react

100 Activation of the SR proteins by matrix stiffness and the subsequent produ

101 This result provides strong evidence that an SR protein can promote recruitment of splicing factors t

102 Depletion of MALAT1 or overexpression of an SR protein changes the AS of a similar set of endogenous

103 downstream 3'ss, which repositions NRS-bound SR proteins closer to the viral PAS.

104 We propose a model in which hnRNP H and SR proteins compete for binding to the NRS.

105 SR proteins constitute a family of pre-mRNA splicing fac

106 SR proteins constitute a widely conserved family of spli

107 The serine-arginine-rich (SR) proteins constitute a conserved family of pre-mRNA s

108 e show that the RS domain of this additional SR protein contacts the 5' splice site specifically in t

109 ular Cell, demonstrate that the RS domain of SR proteins contacts the pre-mRNA branchpoint, indicatin

110 ver an extensive cross-regulatory network of SR proteins controlling their expression via alternative

111 digestion suggests that endogenous EJCs and SR proteins cooperate to promote mRNA packaging and comp

112 ferentiation in P19 cells, illustrating that SR protein-dependent exon skipping may constitute a key

113 upon entry into daughter nuclei, snRNPs and SR proteins do not immediately colocalize in nuclear spe

114 m regulating distinct recycling pathways for SR proteins during mRNP maturation.

115 sis revealed increased expression of several SR proteins during the early response to DNA damage, whi

116 at SRPK2 binds and phosphorylates acinus, an SR protein essential for RNA splicing, and redistributes

117 Mouse orthologues of the human SR proteins exhibit the same unproductive splicing patte

118 Here we critically evaluate members of the SR protein family (SRSF1-7) for their potential to act a

119 SRp38 is a member of the SR protein family and, when dephosphorylated, functions

120 t facilitates the interaction with SRSF3, an SR protein family member that promotes pri-miRNA process

121 uring pregnancy is regulated primarily by an SR protein family member, SRp40.

122 we performed extensive sequence analysis of SR protein family members and combined it with ordered/d

123 much stronger splicing activators than other SR protein family members and their activation potential

124 expansion of putative functions of specific SR protein family members in RNA metabolism downstream o

125 ocytoplasmic shuttling among seven canonical SR protein family members.

126 Among these are the SR protein family of splicing factors and all of the com

127 they both strongly predicted members of the SR protein family to be disordered.

128 ied: p54 (also called SRp54; a member of the SR protein family), human transformer 2 beta (hTra2 beta

129 Here we show that a 35-kDa member of the SR protein family, 9G8, can activate the splicing of alp

130 Compared with other members of the SR protein family, SRSF2 structure has a longer L3 loop

131 icing factor Sfrs1, an arginine/serine-rich (SR) protein family member, during mouse retinal developm

132 ASF/SF2, a member of the serine-arginine (SR) protein family, has two RRM domains (RRM1 and RRM2)

133 , >1000 endogenous mRNAs required individual SR proteins for nuclear export in vivo.

134 ctin, a 26 kDa intra-sarcoplasmic reticulum (SR) protein, forms a quaternary complex with triadin, ca

135 ugh phosphorylation directs serine-arginine (SR) proteins from nuclear storage speckles to the nucleo

136 here is involved in the regulation of E2 and SR protein function in posttranscriptional processing of

137 lts underline the importance of ESE-mediated SR protein function in the process of exon definition, i

138 We show that SR proteins function in coupling transcription to splici

139 SR proteins function in nuclear pre-mRNA processing, mRN

140 gely unexplored, as genetic deletion of many SR protein genes results in embryonic lethality.

141 Negative autoregulation of SR proteins has been proposed to exert homeostatic contr

142 SR proteins have also been reported to cause exon skippi

143 Although shuttling SR proteins have been implicated in mRNA export, prevent

144 Three SR proteins have been previously shown to direct splicin

145 SR proteins have been studied extensively as a family of

146 We found that SR proteins have properties characteristic of intrinsica

147 Although serine-arginine rich (SR) proteins have often been implicated in the positive

148 egulated by a group of serine/arginine rich (SR) proteins, heterogeneous nuclear ribonucleoproteins (

149 entified a previously unknown function of an SR protein (i.e., the stimulation of IRES-mediated trans

150 nal discovery and classification, we catalog SR proteins in 20 model organisms, with a focus on green

151 utoregulatory exon induces expression of all SR proteins in a human cell line.

152 asm, which is critical for nuclear import of SR proteins in a phosphorylation-dependent manner.

153 plicing and reveal an additional function of SR proteins in eukaryotic gene expression.

154 Yeast Npl3 is homologous to SR proteins in higher eukaryotes, a family of RNA-bindin

155 lex set of relationships with multiple other SR proteins in mammalian genomes.

156 ults further suggest a more general role for SR proteins in polyadenylation of cellular mRNAs.

157 This indicates a novel role for SR proteins in promoting RSV polyadenylation in the cont

158 ow they may work to regulate the function of SR proteins in RNA metabolism in mammalian cells.

159 model genes apply generally to activities of SR proteins in the cell.

160 bstantially expands the known roles of human SR proteins in the regulation of a diverse array of RNA

161 s, therefore, highlight the broader roles of SR proteins in vertical integration of gene expression a

162 important implications on the regulation of SR proteins in vivo by the SRPK and Clk/Sty families of

163 ce to address the function and regulation of SR proteins in vivo.

164 ern blot analysis of sarcoplasmic reticulum (SR) proteins in skeletal muscle showed that the absence

165 l SR protein knockout mice, we now show that SR protein-induced exon skipping depends on their preval

166 stable structure to facilitate high-affinity SR protein interactions and phosphorylation rates.

167 SR proteins involved in calcium metabolism, including th

168 SRPK1 phosphorylation, not only of cellular SR proteins involved in regulating alternative splicing

169 Surprisingly, five serine/arginine-rich (SR) proteins involved in messenger RNA splicing, includi

170 Further analyses reveal that loss of one SR protein is accompanied by coordinated loss or compens

171 e and how it triggers the importation of the SR protein is lacking.

172 The FgSrp1 SR protein is likely important for pre-mRNA processing o

173 important for intron splicing and the FgSrp1 SR protein is phosphorylated at five conserved sites in

174 fic dephosphorylation of SRp38 and not other SR proteins is determined largely by the low activities

175 gly, the SRPK family of kinases specific for SR proteins is localized in the cytoplasm, which is crit

176 In this process, the RS domain of SR proteins is thought to directly interact with the RS

177 NMD of mRNAs for splicing activators such as SR proteins is triggered by splicing activation events,

178 nt reductions in the sarcoplasmic reticulum (SR) proteins junctin and triadin-1 and increased SR volu

179 SR protein kinase 1 (SRPK1) phosphorylates approximately

180 rines are processively phosphorylated by the SR protein kinase 1 (SRPK1).

181 n groups, including Kinesin heavy chain, the SR protein kinase cuaba, the cohesin-related gene dPds5/

182 The SR protein kinase in yeast, Sky1p, phosphorylates yeast

183 alternative splicing event controlled by the SR protein kinase SPK-1.

184 Putative effectors include an SR protein kinase, bilobe proteins, TbSAS4, TbRP2, and B

185 early observation that overexpression of an SR protein kinase, such as the CDC2-like kinase 1 (CLK1)

186 the SR protein ASF/SF2 is phosphorylated by SR protein kinases (SRPKs) and Clk/Sty kinases.

187 determined largely by the low activities of SR protein kinases for it compared to other SR proteins.

188 vely phosphorylate substrates is inherent to SR protein kinases.

189 protein homologous to serine-arginine-rich (SR) protein kinases, which are thought to regulate splic

190 embryo fibroblasts derived from conditional SR protein knockout mice, we now show that SR protein-in

191 e residues in the RS domain, indicating that SR proteins may be phosphorylated by different kinases i

192 Bound SR proteins may bridge between the NRS and the 3' LTR an

193 Our results suggest that SR proteins mediate the assembly of nuclear speckles and

194 eased or decreased frequencies of particular SR protein motifs.

195 de the first example of a feedback-regulated SR protein network with evidence of an active homeostati

196 In contrast to the cooperative binding of SR proteins observed on the doublesex splicing enhancer,

197 , the cdc2-like kinases (CLKs) phosphorylate SR proteins on their intrinsically disordered Arg-Ser (R

198 Serine/arginine (SR) proteins, one of the major families of alternative-s

199 ns and upregulating a RBFOX protein and that SR protein overexpression impairs regulated CaMKIIdelta

200 The data imply that an allosteric SR protein-phosphatase platform balances phosphorylation

201 hanistic insights into complex regulation of SR protein phosphorylation and alternative splicing in r

202 However, little is known how SR protein phosphorylation might be regulated during dev

203 e known to inhibit splicing, indicating that SR protein phosphorylation must be tightly regulated in

204 i that bind tightly to RS domains, enhancing SR protein phosphorylation.

205 s to enhanced SRPK nuclear translocation and SR protein phosphorylation.

206 to change alternative splicing and decrease SR-protein phosphorylation by activating protein phospha

207 Serine/arginine-rich (SR) proteins play essential roles in precursor mRNA cons

208 This SR protein plays critical roles in spliceosome assembly,

209 a multi-isomeric family of spectrin-repeat (SR) proteins, predominantly known as nuclear envelope sc

210 Nutritional regulation of other SR proteins presents a regulatory mechanism that could c

211 Unexpectedly, we find that these SR proteins promote both inclusion and skipping of exons

212 SR proteins promote spliceosome formation by recognizing

213 gs, coupled with other established rules for SR proteins, provide a theoretical framework to understa

214 Functional studies of SR protein PTMs have focused exclusively on the reversib

215 ramework to understand the complex effect of SR protein-regulated splicing in mammalian cells.

216 The data establish a new view of SR protein regulation in which SRPK1 and CLK1 partition

217 ent with a model in which hypophosphorylated SR proteins remain stably associated with messenger ribo

218 that modulate the cellular levels of active SR proteins remain to be elucidated.

219 utation that creates a site through which an SR protein represses splicing.

220 We report here that the SR protein SC35 controls cell proliferation during pitui

221 hnRNPs H, F, 2H9, GRSF1, A1, A2, and A3 and SR proteins SC35, SF2, and SRp40 in HEK 293 cells transf

222 ocalizes with snRNPs, but not with a typical SR protein, SC35, during mitosis and following heat shoc

223 isparate functions, showing that a subset of SR proteins seem to bind directly to the histone 3 tail,

224 SR proteins serve multiple roles in the posttranscriptio

225 The SR protein SF2/ASF has been initially characterized as a

226 The SR protein SF2/ASF is an oncoprotein that functions in p

227 hat can act as ESEs in response to the human SR protein SF2/ASF.

228 pression of hnRNPs H, F, 2H9, A1, and A2 and SR proteins SF2 and SRp40 increased nuclear localization

229 that overexpression of hnRNPs A1 and A2 and SR proteins SF2, SC35, and SRp40 causes a dramatic decre

230 esence of ESE motifs recognized by the human SR proteins SF2/ASF, SRp40, SRp55 and SC35.

231 cted to eukaryotes and that all prototypical SR proteins share a single ancient origin, including the

232 report that SRPK1, a ubiquitously expressed SR protein-specific kinase, directly binds to the cochap

233 Activated Akt next branches to SR protein-specific kinases, rather than mTOR, by induci

234 Serine/arginine (SR) protein-specific kinase (SRPK), a family of cell cyc

235 RPK2 belongs to a family of serine/arginine (SR) protein-specific kinases (SRPKs), which phosphorylat

236 tion of protein phosphatase 1 (PP1) with the SR protein splicing factor (SRSF1) to understand the fou

237 nic splicing enhancer (ESE) dependent on the SR protein splicing factor ASF/SF2 or to the creation of

238 se results not only indicate that loss of an SR protein splicing factor can induce cell cycle arrest

239 ASF/SF2 is an SR protein splicing factor that participates in constitu

240 gion for the proper subnuclear storage of an SR protein splicing factor.

241 We report here that two SR protein splicing factors, SRp20 and ASF/SF2, associat

242 SRp38 is an atypical SR protein splicing regulator.

243 ks) that phosphorylate serine-arginine-rich (SR) protein splicing factors.

244 SR proteins (splicing factors containing arginine-serine

245 SR proteins (splicing factors containing arginine-serine

246 at encodes the yeast orthologue of the human SR protein, SRm300/SRRM2.

247 ese sequences bind the serine/arginine-rich (SR) proteins SRp20 and SF2/ASF and the CELF protein CUG-

248 work demonstrates that a nucleo-cytoplasmic SR protein, SRp20, functions in internal ribosome entry

249 The SR protein SRp38 is a general splicing repressor that is

250 , recent studies have shown that one unusual SR protein, SRp38, serves, contrastingly, as a splicing

251 xon 2 and 10 splicing: serine/arginine-rich (SR) proteins SRp55, SRp30c, and htra2beta1.

252 This ESE binds specifically to the cellular SR protein SRp75.

253 cytoplasmic-nuclear translocalization of the SR protein SRSF1 is regulated by multisite phosphorylati

254 Our analyses revealed that the prototypical SR protein SRSF1 recognizes clusters of GGA half-sites i

255 The shuttling Serine/Arginine rich (SR) protein SRSF1 (previously known as SF2/ASF) is a spl

256 e report global analyses of two prototypical SR proteins, SRSF1 (SF2/ASF) and SRSF2 (SC35), using spl

257 rds this end, we sought to determine how two SR proteins-SRSF3 and SRSF7, regulators of pre-mRNA spli

258 Overexpression of various SR proteins strongly enhanced NMD, and this effect requi

259 nucleus by forming a stable complex with the SR protein substrate and appropriating its NLS for trans

260 isordered structure for several functions of SR proteins, such as for spliceosome assembly and for in

261 ble functional similarities between CFIm and SR proteins suggest that interactions between RS-like do

262 hat two members of the serine/arginine-rich (SR) protein superfamily (ASF/SF2 and SC35) act antagonis

263 Binding sites for serine/arginine rich (SR) proteins tended to be exonic whereas heterogeneous r

264 of SR genes to produce different isoforms of SR proteins that are likely to have altered function(s)

265 dentified PfSR1 as a bona fide Ser/Arg-rich (SR) protein that shuttles between the nucleus and cytopl

266 PK1 phosphorylation of host serine-arginine (SR) proteins that have critical roles in mRNA metabolism

267 the modular nature of the various domains in SR proteins, the proteins produced from splice variants

268 ough the activation of serine/arginine rich (SR) proteins, the splicing factors involved in the produ

269 ough pseudo-RRMs are crucial for activity of SR proteins, their mode of action was unknown.

270 litated by a subset of serine/arginine-rich (SR) proteins through activation of the optimized 3' spli

271 acks the mechanism to release phosphorylated SR proteins, thus functionally inactivating the splicing

272 essive mechanism--a process that directs the SR protein to the nucleus and influences protein-protein

273 ltimerize with one another and with numerous SR proteins to form megadalton sized complexes in which

274 To map binding of SR proteins to lincRNAs, we performed iCLIP with SRSF2,

275 sm involves cotranscriptional recruitment of SR proteins to RNAP II transcripts.

276 tagonizes binding of a serine/arginine-rich (SR) protein to an exonic splicing enhancer, thereby inhi

277 f a heterotrimeric complex consisting of the SR proteins Tra, Tra2 and 9G8.

278 The N-terminus and SID equally enhance SR protein turnover by altering the stability of several

279 ng the IkappaB-alpha S32/36A superrepressor (SR) protein under control of the mouse mammary tumor vir

280 bidopsis, pre-mRNAs of serine/arginine-rich (SR) proteins undergo extensive alternative splicing (AS)

281 icate that posttranslational modification of SR proteins underlies the regulation of their mRNA expor

282 eir well-established roles in mRNA splicing, SR proteins use disordered protein-protein interactions

283 ines in arginine-serine-rich (RS) domains of SR proteins using a directional, C-to-N-terminal mechani

284 of the intronic repressor and recruitment of SR proteins via the SR recruitment sequence of the bifun

285 on in the phosphorylation status of specific SR proteins was detected following the down-regulation o

286 To investigate the evolution of SR proteins, we infer phylogenies for more than 12,000 R

287 ght into structure-function relationships of SR proteins, we performed extensive sequence analysis of

288 der predictions indicated that RS domains of SR proteins were completely unstructured.

289 However, SR proteins were first discovered as factors that intera

290 inding sites or a downstream 3' splice site, SR proteins were sufficient to stimulate polyadenylation

291 acid composition and sequence complexity of SR proteins were very similar to those of the disordered

292 SRSF2 is a prototypical SR protein which plays important roles in the alternativ

293 to facilitate the release of phosphorylated SR proteins, which then promotes efficient splice-site r

294 providing binding sites for serine-arginine (SR) proteins, which contain an arginine-serine-rich (RS)

295 orylation, suggesting that engagement of the SR protein with exonic splicing enhancers can regulate p

296 Our results strongly suggest that SR proteins with a pseudo-RRM frequently regulate splici

297 show that SON facilitates the interaction of SR proteins with RNA polymerase II and other key spliceo

298 e that the exclusive association of U1 snRNP/SR proteins with RNAP II positions these splicing factor

299 alent interactions between ASF/SF2 and other SR proteins with the 5' ends of SMN1 and SMN2 exon 7.

300 scopy to identify interactions of individual SR proteins with the U1 small nuclear ribonucleoprotein