ライフサイエンスコーパス: T4 DNA ligase

1 er incubation times than required when using T4 DNA ligase.

2 , with ligation of these strands mediated by T4 DNA ligase.

3 , and the resulting termini are ligated with T4 DNA ligase.

4 timulation of end-joining in the presence of T4 DNA ligase.

5 equence and the RNA probe are linked using a T4 DNA ligase.

6 e ligation reactions using T4 RNA ligase and T4 DNA ligase.

7 s as tools to determine the DNA footprint of T4 DNA ligase.

8 , which allows for cyclization/ligation with T4 DNA ligase.

9 ends can undergo self-ligation catalyzed by T4 DNA ligase.

10 r C, proliferating cell nuclear antigen, and T4 DNA ligase.

11 ders of magnitude higher fidelity than viral T4 DNA ligase.

12 ion of the strains containing high levels of T4 DNA ligase.

13 degrees C; (iii) it has higher fidelity than T4 DNA ligase.

14 ded by DNA exonucleases or ligated by T3 and T4 DNA ligases.

15 s and illustrates examples using the Taq and T4 DNA ligases.

16 nerated cysteine at the N terminus of either T4 DNA ligase (56 kDa) or thioredoxin (12 kDa) resulted

17 ct 5'-dRP group release assays, we show that T4 DNA ligase, a representative ATP-dependent DNA ligase

18 could be ligated to an acceptor duplex using T4 DNA ligase, a result that suggests that the C-C cross

19 s, the reaction mixtures were incubated with T4 DNA ligase and ATP and then used to transfect SOS-ind

20 e present a system which uses thermotolerant T4 DNA ligase and no UV.

21 Ligation by both T4 DNA ligase and Rhodothermus marinus thermophilic DNA

22 However, T4 DNA ligase and RtcA can use 3'-phosphorylated nicks i

23 oligonucleotide of length six or less, while T4 DNA ligase and T7 DNA ligase are both able to join he

24 ving the repair of DNase I digested DNA with T4 DNA ligase and the Klenow fragment of DNA polymerase

25 lently linked to the restriction fragment by T4 DNA ligase, and amplification is carried out under co

26 promotes DNA end-joining in the presence of T4 DNA ligase, and this property is mediated by the C-te

27 ed, amplified by long PCR, self-ligated with T4 DNA ligase, and transformed into E. coli.

28 of a DNA linker to the tailed cDNA by use of T4 DNA ligase; and (iv) PCR using a nested, gene-specifi

29 n of base excision repair can be mediated by T4 DNA ligase as well as human DNA ligase I or ligase II

30 reaction instead of the existing chemical or T4 DNA ligase-based methods allows quantitative conversi

31 fficiency on nick position demonstrates that T4 DNA ligase bound asymmetrically to its DNA substrate.

32 9 bp and 94 bp dsDNA fragments, we find that T4 DNA ligase can have a previously unknown effect.

33 We describe the application of T4 DNA ligase-catalyzed DNA templated oligonucleotide po

34 The development and in-depth analysis of T4 DNA ligase-catalyzed DNA templated oligonucleotide po

35 We have developed a method for the T4 DNA ligase-catalyzed DNA-templated polymerization of

36 T4 DNA ligase catalyzes phosphodiester bond formation be

37 T4 DNA ligase catalyzes the template-dependent ligation

38 ge and small DNA substrates can be sealed by T4 DNA ligase, confirming the formation of nicked duplex

39 DNA adenylate structure is generated by the T4 DNA ligase during enzymatic DNA ligation.

40 he stereochemical preferences of AP endo and T4 DNA ligase for phosphorothioate substrates, we show t

41 that carry a plasmid containing an inducible T4 DNA ligase gene.

42 Ring closure analysis with T4 DNA ligase has confirmed the general magnitude of the

43 uilibrium constants for the adenylylation of T4 DNA ligase have been measured at 10 pH values.

44 on linear pUC19 plasmid DNA was treated with T4 DNA ligase in solutions containing 2 mM Mg2+ over a r

45 ay system to show increased sensitivity over T4 DNA ligase in the specific detection of a target mRNA

46 enzymatically ligated (by the commonly used T4 DNA ligase) into monomeric ssDNA circles at high conc

47 The AP lyase activity of T4 DNA ligase is inhibited in the presence of ATP, sugge

48 mulation is specific to DNA ligase I because T4 DNA ligase is not affected.

49 T4 DNA ligase is used in standard cyclization assays to

50 method based on TdT terminal transferase and T4 DNA ligase-mediated barcoding strategy and single-cel

51 , indicating that the rate-limiting step for T4 DNA ligase nick sealing is not a chemical step but ra

52 ng of two fluorescently labeled DNA with the T4 DNA ligase on the single-molecule level.

53 SplintR Ligase is 100X faster than either T4 DNA Ligase or T4 RNA Ligase 2 for RNA splinted DNA li

54 The 58kDa T4 DNA ligase possessing an N-terminal cysteine was gene

55 1 nM at 25 degrees C under conditions where T4 DNA ligase produced only 5'-adenylylated DNA with a 2

56 ce of reactions mimics the final step of the T4 DNA ligase reaction.

57 as calculated using the reversibility of the T4 DNA ligase reaction.

58 We show that the T4 DNA ligase repairs sticky ends more efficiently than

59 Therefore, the T4 DNA ligase strains are useful for cloning linear DNAs

60 Unlike reactions that use T4 DNA ligase, this protocol does not require synthesis

61 vities of terminal DNA transferase (TdT) and T4 DNA ligase to add unique cell barcodes to DNase-diges

62 rase and dNTPs or by enzymatic ligation with T4 DNA ligase to another oligonucleotide opposite a comp

63 The hemiprobes are then ligated by a T4 DNA ligase to form a full probe that serves as a temp

64 n be linked to each other by incubation with T4 DNA ligase to form a full probe that serves as a temp

65 ensely functionalized nucleic acids by using T4 DNA ligase to mediate the DNA-templated polymerizatio

66 Using T4 DNA ligase under specific experimental conditions, we

67 interactions is confirmed with both Tth and T4 DNA ligases upon examination of base pairs containing

68 ng by both ASFV DNA ligase and bacteriophage T4 DNA ligase was determined in the steady state for sub

69 The enzyme T4 DNA ligase was used to catalyze the formation of a ph

70 Ligation experiments using bacteriophage T4 DNA ligase were carried out with purine lesions in fo

71 d by ligation of three to ten hexamers using T4 DNA ligase) were all of high quality, with no spuriou

72 n-ligation strategy based on USER Enzyme and T4 DNA ligase, which allows the simultaneous and seamles

73 ed enzyme for this reaction is bacteriophage T4 DNA ligase, which requires ATP as the energy source t

74 of this previously undiscovered property of T4 DNA ligase with canonical nucleosides are discussed.

75 p at the modification site that is sealed by T4-DNA ligase, yielding a product strand missing the mod