ライフサイエンスコーパス: T4SS

1 T4SS foci likely use an existing helical scaffold during

2 T4SS function and cagY in H pylori from C57BL/6 mice wer

3 T4SS proteins expressed by in vitro transcription and tr

4 T4SSs are versatile systems that can transport not only

5 Using a bioinformatics-guided approach, 234 T4SS candidate substrates were identified.

6 The pKM101 plasmid encodes a T4SS similar to the well-studied model systems from Agro

7 translocation and downstream apoptosis in a T4SS-dependent manner.

8 COG-dependent trafficking via delivery of a T4SS effector to promote rBCV biogenesis and intracellul

9 f cell-autonomous host immunity and reveal a T4SS-translocated L. pneumophila phytase that counteract

10 That a T4SS can have anti- and prokilling effects on different

11 man lung epithelial cells, indicating that a T4SS effector(s) has antiapoptotic activity.

12 there was a lack of inflammasome activation, T4SS-dependent cytokine responses, and robust type I int

13 of this study was to determine if additional T4SS proteins are immunogenic for animals immunized with

14 VirB8 that is an essential component of all T4SSs.

15 t are required for both pilus biogenesis and T4SS function and reveal that these processes can be unc

16 as one operon, indicating the ribA gene and T4SS operon 1 are co-regulated by both wBmxR1 and wBmxR2

17 s strain, infectious dose, growth phase, and T4SS dependent.

18 , while low c-di-GMP levels unleash T6SS and T4SS to advance plant colonization.

19 Accordingly, antiapoptotic T4SS effector proteins, like AnkG, have been identified.

20 ave not been assessed for their potential as T4SS substrates.

21 train JR32 contains two virulence-associated T4SSs, the Dot/Icm and Lvh T4SSs.

22 critical role of host TNF and the bacterial T4SS in pathogenesis.

23 ctural diversity that exists among bacterial T4SSs.

24 To assess relevance to human beings, T4SS function and cagY recombination were assessed in st

25 agM, and cag3 mutants were defective in both T4SS function and pilus formation; complemented mutants

26 tic protein CD98hc as a partner for Brucella T4SS subunit VirB2.

27 Although the importance of Brucella T4SS is clear, little is known about its interactions wi

28 ime that the H. pylori cancer-associated cag T4SS is required for TLR9 activation and that H. pylori

29 The H. pylori Cag T4SS includes a large membrane-spanning core complex con

30 molecular architecture of the H. pylori Cag T4SS is substantially more complex than that of prototyp

31 The Helicobacter pylori Cag T4SS translocates CagA, a bacterial oncoprotein, into ga

32 ort the structure of a membrane-spanning Cag T4SS assembly, which we describe as three sub-assemblies

33 omponents of a type IV secretion system (Cag T4SS).

34 These data suggest that Cag T4SS-dependent delivery of nonprotein bacterial constitu

35 ing to the structure and function of the Cag T4SS and its role in gastric cancer pathogenesis.

36 lori DNA is actively translocated by the cag T4SS to engage this host receptor.

37 12 protein represses the activity of the cag T4SS, as evidenced by the gastric cell "hummingbird" phe

38 that S100A12 represses biogenesis of the cag T4SS.

39 H. pylori and its cag-T4SS exploit alpha5beta1 integrin as a receptor for CagA

40 carry the cag type IV secretion system (cag-T4SS) to inject the cytotoxin-associated antigen A (CagA

41 ted by beta1 integrin interaction of the cag-T4SS.

42 eukaryotic features are considered candidate T4SS substrates.

43 risons with other structurally characterized T4SSs.

44 acellular replication, we isolated 20 clonal T4SS substrate mutants using the Himar1 transposon and t

45 rack intracellular replication, we confirmed T4SS-dependent intracellular growth of B. neotomae in ma

46 system (T4SS), a relative of the conjugative T4SS, we demonstrate that catalytically active Osa block

47 Conjugative T4SSs comprise 12 proteins named VirB1-11 and VirD4 that

48 T4SS can be grouped with F-type conjugative T4SSs based on homology.

49 ween proteins with homology to the conserved T4SS outer membrane core proteins and F-type-specific pr

50 s and found that the four operons containing T4SS genes are transcribed at very different levels.

51 nd post-translational mechanisms controlling T4SS gene expression and DNA secretion.

52 rosophila TNF homolog Eiger and the Coxiella T4SS are implicated in the pathogenesis of C. burnetii i

53 Protein substrates of the Coxiella T4SS are predicted to facilitate the biogenesis of a pha

54 asmic tails, VirB8 and VirD4, or cytoplasmic T4SS substrate proteins, VirD2, VirE2, and VirF, localiz

55 Thus, S. maltophilia VirB/D4 T4SS appears to secrete multiple effectors capable of mo

56 e IVA secretion system (VirB/VirD4 [VirB/D4] T4SS) that is highly conserved among S. maltophilia stra

57 study of type IV secretion system-dependent (T4SS) pathogenesis in the Brucella genus.

58 dies indicate that substrates of the Icm/Dot T4SS are translocated to the host cytosol in an unfolded

59 n mechanism by which H. pylori downregulates T4SS function during murine infection, loss of function

60 n motor to extract pilins from the IM during T4SS biogenesis.

61 e genetic elements and chromosomally encoded T4SS from G+ and G- bacteria, we propose a new classific

62 abolished functionality of the pCF10-encoded T4SS as monitored by pCF10 conjugative transfer.

63 rotein substrates through the pKM101-encoded T4SS.

64 lts suggest that C. burnetii plasmid-encoded T4SS substrates play important roles in subversion of ho

65 recombination in cagY, which is an essential T4SS gene.

66 nserved among those bacteria also expressing T4SS.

67 intracellular bacterial pathogens expressing T4SSs as well as in many slow-growing soil and aquatic b

68 ome non-cagPAI genes, are also essential for T4SS function.

69 (Bacterial secretion effector predictor for T4SS), and we show our ensemble classifier clearly outpe

70 he cag PAI that are known to be required for T4SS function and investigated whether these genes were

71 and the adjacent vir genes predicted to form T4SS, in addition to the status of cag pathogenicity isl

72 ding direct genetic evidence of a functional T4SS in C. burnetii.

73 he absence of thymidine but has a functional T4SS, resisted clearance in G. mellonella up to 18 h pos

74 Unlike other gonococcal T4SS proteins we have investigated, protein levels of th

75 These results suggest that the gonococcal T4SS may be present in single copy per cell and that sma

76 imilar to other F-like T4SSs, the gonococcal T4SS requires a putative membrane protein, TraG, for DNA

77 showed that in contrast with the gonococcal T4SS, the meningococcal T4SS does not secrete DNA, nor d

78 th the unique function of the N. gonorrhoeae T4SS as well as generic features of F-type T4SSs.

79 The N. gonorrhoeae T4SS can be grouped with F-type conjugative T4SSs based

80 Using the m-ARIA, significantly higher T4SS and VAS scores were obtained when comparing severe

81 aces between the key proteins of the Dot/Icm T4SS core complex and provides a framework for understan

82 The Legionella pneumophila Dot/Icm T4SS injects approximately 300 protein effector proteins

83 ly or indirectly in translocation of Dot/Icm T4SS protein substrates.

84 9-fold increases in translocation of Dot/Icm T4SS substrates, LegS2/SplY and LepB.

85 tructions of the core complex of the Dot/Icm T4SS that includes a symmetry mismatch between distinct

86 or amoeba and HeLa cells and loss of Dot/Icm T4SS-mediated contact haemolysis of erythrocytes.

87 ophagosome interactions by using the Dot/Icm T4SS.

88 ed into macrophage-like cells by the Dot/Icm T4SS.

89 e associated with dysfunction of the Dot/Icm T4SS.

90 osely related Legionella pneumophila Dot/Icm T4SS.

91 eins that included components of the Dot/Icm T4SS.

92 Notably, 26 identified T4SS substrates are hypothetical proteins without predic

93 The discovery of novel immunogenic T4SS proteins recognized by outbred individuals with com

94 d from a patient over time showed changes in T4SS function that were dependent on recombination in ca

95 cagY and cagC mutants were defective in T4SS function but retained the capacity for pilus format

96 for pilus formation, and the role of pili in T4SS function is unclear.

97 tion events typically lead to a reduction in T4SS function in mouse and primate models.

98 TPase proteins revealed that they are indeed T4SS secreted substrates.

99 tions in the GGI, other strains carry intact T4SS genes and may produce functional secretion systems.

100 spase-1-dependent pyroptosis may require its T4SS and activation of the TLR-2 and NLRP3 signaling pat

101 Similar to other F-like T4SSs, the gonococcal T4SS requires a putative membrane

102 agY in 534 mouse-passaged isolates that lost T4SS function, defined as a normalized interleukin-8 (IL

103 f dot/icm virulence defects requires the Lvh T4SS and is associated with a >10-fold induction of LpnE

104 ontrast to dot/icm mutants for which the Lvh T4SS was required, reversal for the DeltalpnE or the Del

105 h independent functioning of Dot/Icm and Lvh T4SSs or functional substitution of the Lvh VirD4 protei

106 ulence-associated T4SSs, the Dot/Icm and Lvh T4SSs.

107 bacterial poles, as is consistent with many T4SS substrates being retained on the phagosomal membran

108 A. marginale T4SS proteins VirB2, VirB4-1, VirB4-2, VirB6-1, VirB7, V

109 fine requirements for phage-contact-mediated T4SS activation and phage penetration.

110 with the gonococcal T4SS, the meningococcal T4SS does not secrete DNA, nor does it confer Ton-indepe

111 In wBm, most T4SS genes are contained in two operons.

112 Multiple T4SS effectors localize to and/or disrupt the endoplasmi

113 These data lead to a model with multiple T4SSs around the bacterial cell that likely facilitate h

114 e intracellular replication of a B. neotomae T4SS virB4 mutant was rescued and baseline levels of int

115 The C. burnetii NMII T4SS translocates bacterial products into C57BL/6 macrop

116 r an isogenic cagE (-) mutant (nonfunctional T4SS).

117 ulated by coinfection with wild-type but not T4SS mutant L. pneumophila Using confocal microscopy, it

118 lpha in response to T4SS-sufficient, but not T4SS-deficient, L. pneumophila.

119 ngle copy per cell and that small amounts of T4SS proteins TraK and TraB are sufficient for DNA secre

120 VirB4 in prototypical T4SSs) were capable of T4SS core complex assembly but defective in CagA translo

121 Deletion of T4SS genes did not affect association with or invasion o

122 ted for the design of specific inhibitors of T4SS function.

123 Loss of T4SS function and cagY recombination were more pronounce

124 1-/- mice, showed that cagY-mediated loss of T4SS function requires a T-helper 1-mediated immune resp

125 une response in cagY-dependent modulation of T4SS function.

126 utrophils both are the primary recipients of T4SS-translocated effectors and harbor viable L. pneumop

127 dated LC3 levels were elevated regardless of T4SS activity, no p62 turnover was observed during C. bu

128 haracterize the expression and regulation of T4SS genes and found that the four operons containing T4

129 unctions as an immune-sensitive regulator of T4SS function.

130 at C. burnetii encodes a large repertoire of T4SS substrates that play integral roles in host cell su

131 nd environmental signals to transcription of T4SS genes are increasingly understood, it remains funda

132 he biogenesis, architecture, and function of T4SSs.

133 y step of MS2 engagement with the F-pilus or T4SS.

134 TraK nor TraB required the presence of other T4SS components for proper localization.

135 by the corresponding protein from the other T4SS, we aimed to identify features associated with the

136 d interactions shared by homologs from other T4SSs as well as new and described interactions between

137 B7 homolog) interacts with TraK, as in other T4SSs.

138 derstand how ImaA could be affecting cag PAI T4SS activity at the host cell interface, we utilized th

139 (HP0289) decreases the action of the cag PAI T4SS via tempering the bacterium's interaction with alph

140 y response that was dependent on the cag PAI T4SS; here we extend those findings to show that the ele

141 ntial component of the pCF10-encoded Prg/Pcf T4SS and that its hydrolase domains coordinate their act

142 The pKM101 T4SS, thus, deploys alternative routing pathways for the

143 sembly, but also to activate the Tra(pKM101) T4SS via interactions with the ATPase energy center posi

144 ame interactions were observed for F-plasmid T4SS proteins and when one interaction partner was repla

145 the well-established Legionella pneumophila T4SS secretion model.

146 ion levels and localization of two predicted T4SS outer membrane proteins, TraK and TraB, in the wild

147 antially more complex than that of prototype T4SSs in other bacterial species.

148 to VirB11, VirD4, and VirB4 in prototypical T4SSs) were capable of T4SS core complex assembly but de

149 t differ markedly from those of prototypical T4SSs include an expanded OMCC and unexpected symmetry m

150 Compared to prototypical T4SSs, the Dot/Icm assembly is much larger, containing ~

151 H pylori T4SS function was assessed by measuring CagA translocati

152 esults reveal that assembly of the H. pylori T4SS core complex is dependent on incorporation of inter

153 sential cagPAI genes implicated in H. pylori T4SS function, most commonly cag5, cag10, and cagA While

154 With a partially reconstituted T4SS in vitro, we show that Osa degrades T-DNA in the T-

155 lus formation; complemented mutants regained T4SS function and the capacity for pilus formation.

156 Brucella pathogenesis and found a remarkable T4SS-dependent interplay between Brucella and Legionella

157 This coevolution of the rvh T4SS and cell envelope morphology is probably driven by

158 cterization of several components of the rvh T4SS, as well as its putative regulators and substrates.

159 le event of ancestral acquirement of the rvh T4SS, likely from a nonalphaproteobacterial origin.

160 faciens, the Rickettsiales vir homolog (rvh) T4SS is characterized primarily by duplication of severa

161 assessed using the Total Four Symptom Score (T4SS), and the severity was evaluated by both ARIA sever

162 In order to achieve this, several T4SS effectors target regulators of membrane trafficking

163 oint to a novel function for these signature T4SS ATPases in mediating early steps of type IV secreti

164 ts suggest that ElpA is a pathotype-specific T4SS effector that influences ER function during C. burn

165 hrough the Icm/Dot type IV secretion system (T4SS) and approximately 300 different "effector" protein

166 he activity of the Type IV secretion system (T4SS) and subsequently the capacity of A. tumefaciens to

167 e cell biology and type IV secretion system (T4SS) dependence of B. neotomae intracellular replicatio

168 acterium harbors a type IV secretion system (T4SS) highly similar to the Dot/Icm of Legionella pneumo

169 s translocated by a type 4 secretion system (T4SS) into gastric epithelial cells and activates oncoge

170 , and it encodes a type IV secretion system (T4SS) involved in DNA release.

171 e that the Coxiella type 4 secretion system (T4SS) is critical for the formation of the Coxiella-cont

172 The Dot/Icm type IV secretion system (T4SS) is one of the key virulence factors required for i

173 eat-killed NMII and type 4 secretion system (T4SS) mutant NMII were unable to induce B1a cell death a

174 The Dot/Icm type IV secretion system (T4SS) of Legionella pneumophila is crucial for the patho

175 ly of the Dot/Icm type IVb secretion system (T4SS) of Legionella pneumophila is dependent on correct

176 ted by the Dot/Icm type IV secretion system (T4SS) of several Legionella pneumophila strains.

177 The Type IV secretion system (T4SS) plays an important role in bacteria-host interacti

178 encodes a Dot/Icm type IV secretion system (T4SS) predicted to deliver to the host cytosol effector

179 s, including three type IV secretion system (T4SS) proteins, VirB9-1, VirB9-2, and VirB10.

180 mid pCF10 encode a type IV secretion system (T4SS) required for conjugative transfer.

181 H. pylori carry a type IV secretion system (T4SS) responsible for the injection of the oncoprotein C

182 mbers of the large type IV secretion system (T4SS) superfamily.

183 is a specialized type IVB secretion system (T4SS) that delivers effectors essential for intracellula

184 d, which encodes a type IV secretion system (T4SS) that injects the CagA oncoprotein into host cells.

185 nd (GGI) encodes a type IV secretion system (T4SS) that is found in most strains of N. gonorrhoeae.

186 cag PAI encodes a type IV secretion system (T4SS) that mediates delivery of the CagA effector protei

187 d, which encodes a type IV secretion system (T4SS) that translocates a pro-inflammatory and oncogenic

188 equire the Dot/Icm Type IV Secretion System (T4SS) to deliver effector proteins into host cells.

189 gen uses a Dot/Icm type IV secretion system (T4SS) to deliver effector proteins to the host cytoplasm

190 thogens that use a type IV secretion system (T4SS) to escape host defenses and create a niche in whic

191 tii uses a Dot/Icm type IV secretion system (T4SS) to generate a phagolysosome-like parasitophorous v

192 rget the conserved type IV secretion system (T4SS) to identify conserved, immunogenic membrane protei

193 gonorrhoeae uses a type IV secretion system (T4SS) to secrete chromosomal DNA into the medium, and th

194 gonorrhoeae uses a type IV secretion system (T4SS) to secrete chromosomal DNA into the surrounding mi

195 lication (Dot/Icm) type 4b secretion system (T4SS) to silence the host innate immune response during

196 quires the Dot/Icm type IV secretion system (T4SS) to translocate a cohort of effector proteins into

197 mploying a Dot/Icm type IV secretion system (T4SS) to translocate effector proteins that direct the f

198 ts dot/icm-encoded type IV secretion system (T4SS) to translocate effector proteins that promote its

199 n pathogens, use a type IV secretion system (T4SS) to translocate effectors directly into the cytosol

200 cterium VirB/VirD4 type IV secretion system (T4SS) undergoes a structural transition in response to s

201 umefaciens VirB/D4 type IV secretion system (T4SS), a relative of the conjugative T4SS, we demonstrat

202 encodes CagA and a type IV secretion system (T4SS), induce more severe disease outcomes.

203 nregulation of the type IV secretion system (T4SS), typically by recombination in cagY, which is an e

204 nds on its Dot/Icm type IV secretion system (T4SS), which delivers more than 300 effector proteins in

205 cular, the Dot/Icm type IV secretion system (T4SS), which is essential to establish a replication-per

206 cer risk is the cag type 4 secretion system (T4SS), which translocates the oncoprotein CagA into host

207 nvironment using a type IV secretion system (T4SS).

208 sm using a Dot/Icm type IV secretion system (T4SS).

209 the H. pylori cag type IV secretion system (T4SS).

210 ds on a functional type IV secretion system (T4SS).

211 ctivity of the cag type IV secretion system (T4SS).

212 osol via a Dot/Icm type IV secretion system (T4SS).

213 y determinant, the type IV secretion system (T4SS).

214 enotypes require a type IV secretion system (T4SS).

215 of the VirB/VirD4 type IV secretion system (T4SS).

216 ted by the Dot/Icm type IV secretion system (T4SS).

217 of the VirB/VirD4 type IV secretion system (T4SS).

218 researchers is the type IV secretion system (T4SS).

219 nce (vir) -induced type IV secretion system (T4SS).

220 e of the F-encoded type IV secretion system (T4SS).

221 st cells through a type IV secretion system (T4SS).

222 a highly conserved type IV secretion system (T4SS).

223 y island (cag PAI) type IV secretion system (T4SS).

224 redicted to form a type IV secretory system (T4SS) with vir genes around dupA (dupA cluster).

225 udied conjugative type IV secretion systems (T4SS) are of Gram-negative (G-) origin.

226 en facilitated via type 4 secretion systems (T4SS), which frequently are encoded on conjugative plasm

227 Type IV secretion systems (T4SSs) are large multisubunit translocons, found in both

228 Bacterial type IV secretion systems (T4SSs) are molecular machines that can mediate interbact

229 ed with bacterial type IV secretion systems (T4SSs) are thought to generate localized lesions in the

230 Bacterial type IV secretion systems (T4SSs) can mediate conjugation.

231 tive bacteria use type IV secretion systems (T4SSs) for a variety of macromolecular transport process

232 nts (MGEs) encode type IV secretion systems (T4SSs) known as conjugation machines for their transmiss

233 DNA elements via type IV secretion systems (T4SSs) to bacterial or eukaryotic target cells.

234 subfamily of the type IV secretion systems (T4SSs), termed the conjugation systems, transmit mobile

235 rulence-associated type 4 secretion systems (T4SSs), the Dot/Icm type 4B (T4BSS) and the Lvh type 4A

236 r, is mediated by type IV secretion systems (T4SSs).

237 We propose that T4SS-encoded, pilus-independent attachment mechanisms ma

238 The T4SS from Neisseria gonorrhoeae possesses the unique abi

239 The T4SS is essential for parasitophorous vacuole formation,

240 The T4SS is required for PV expansion and prevention of apop

241 The T4SS translocates effector proteins, or substrates, into

242 The T4SS, composed of a complex of proteins spanning the inn

243 In addition, the T4SS is important in the initial stages of biofilm devel

244 with strains of Pseudomonas aeruginosa, the T4SS promoted the growth of S. maltophilia and reduced t

245 gulate interactions between integrin and the T4SS and thus alter the host inflammatory strength.

246 tural biology of both the relaxosome and the T4SS.

247 eins are delivered into the host cell by the T4SS, the function of the majority is unknown.

248 translocation signal and was secreted by the T4SS, while the N-terminal portion of the protein was no

249 ch allowed us to track cells injected by the T4SS.

250 In the current study, we further defined the T4SS effector repertoire encoded by the C. burnetii QpH1

251 haplotypes further justifies evaluating the T4SS as a potential vaccine candidate for pathogenic bac

252 otypical H. pylori pathogenicity factor, the T4SS, in the up-regulation of miR-155 in BMMs.

253 cagY is an essential gene in the T4SS and has an unusual DNA repeat structure that predic

254 Mat-gRNA complex out of the virion into the T4SS channel, causing a torsional stress that breaks the

255 gene cagY encodes for a key component of the T4SS and can undergo gene rearrangements.

256 irst report of co-regulation of genes of the T4SS and riboflavin biosynthesis pathway.

257 des the first link between expression of the T4SS apparatus and intracellular survival of gonococci.

258 are detectable, structural components of the T4SS are present at very low levels, suggestive of uncha

259 hen identified distinct contributions of the T4SS ATPase subunits to the pilin structural organizatio

260 etraction engages with the distal end of the T4SS channel at the cell surface.

261 In this study, we examined the role of the T4SS in mediating PV interactions with autophagosomes.

262 n strains and in a strain lacking all of the T4SS proteins.

263 In all cases, the effect of the T4SS required S. maltophilia contact with its target.

264 ns interacting with DotF, a component of the T4SS, and (ii) bioinformatic approaches to retrieve cand

265 the promoter region of several genes of the T4SS.

266 ion factors was shown to be dependent on the T4SS, as a Dot/Icm mutant showed reduced nuclear translo

267 plete picture of how H. pylori regulates the T4SS during animal colonization, we examined cagY in 534

268 lower level of apoptosis, implying that the T4SS also elaborates a proapoptotic factor(s).

269 and PCR-based analysis demonstrated that the T4SS contributed to efficient early infection of the liv

270 sion of nucleoprotein substrates through the T4SS.

271 The substrate is then targeted to the T4SS for export into a recipient cell.

272 enotrophomonas genus, is most similar to the T4SS of Xanthomonas To define the role(s) of this T4SS,

273 unexpectedly displayed a fold similar to the T4SS VirB8 proteins from Agrobacterium tumefaciens and B

274 in consisting of beta-lactamase fused to the T4SS-translocated effector RalF, which allowed us to tra

275 When the T4SS was activated 24 h after infection, autophagosome r

276 The vacuole was decorated with the T4SS effector and LCV marker SidC.

277 To determine if any of these T4SS substrates are necessary for intracellular replicat

278 tified that regulates translation of a third T4SS operon.

279 This T4SS functions to secrete single-stranded DNA that is ac

280 of Xanthomonas To define the role(s) of this T4SS, we constructed a mutant of strain K279a that is de

281 Genes encoding three T4SS-associated, putative hydrolases, Lactococcus lactis

282 ies of VirB11 mutants established that three T4SS-mediated processes, DNA transfer, protein transfer,

283 They report that R17 penetrates only through T4SS channels engaged for delivery of their plasmid carg

284 rleukin-8 (IL-8) secretion are attributed to T4SS-dependent delivery of lipopolysaccharide metabolite

285 GFP fusions to T4SS proteins with cytoplasmic tails, VirB8 and VirD4, o

286 n the GGI but instead was directly linked to T4SS structural components in a manner independent of th

287 and p62 localized to wild-type PV but not to T4SS mutant organism-containing phagosomes in human macr

288 factor and interleukin-1alpha in response to T4SS-sufficient, but not T4SS-deficient, L. pneumophila.

289 The stability and steady-state levels of two T4SS structural proteins were affected by a homolog of t

290 s, suggesting an interaction between the two T4SSs in producing Legionella virulence phenotypes.

291 ew and described interactions between F-type T4SS-specific proteins.

292 e T4SS as well as generic features of F-type T4SSs.

293 To better understand T4SS function, we analyzed the localization of its struc

294 us and that a highly conserved yet versatile T4SS secretes an exceptional number of different protein

295 omologous to the well-studied archetypal vir T4SS of Agrobacterium tumefaciens, the Rickettsiales vir

296 bortus uses a type IV secretion system (VirB T4SS) to generate a replication-permissive Brucella-cont

297 Here we show that the VirB T4SS effector BspB contributes to rBCV biogenesis and Br

298 rB8-1, the first gene in operon 1 of the wBm T4SS.

299 ed and replicated within the larvae, whereas T4SS mutants were rapidly cleared.

300 ATPases that are universally associated with T4SSs.