ライフサイエンスコーパス: TAL effector

1 ays of polymorphic amino acid repeats in the TAL effectors.

2 eria represents an unprecedented function of TAL effectors.

3 third mechanism of rice resistance involving TAL effectors.

4 ere experimentally proven targets of natural TAL effectors.

5 factors called transcription activator-like (TAL) effectors.

6 et activity of transcription activator-like (TALs) effectors.

7 EBE variants that cannot be recognized by TAL effectors abrogate induction, causing resistance.

8 ription factors in plant cells; however, how TAL effectors activate host transcription is unknown.

9 Plants counter with TAL effector-activated executor resistance genes, which

10 bles prediction of genomic binding sites for TAL effectors and customization of TAL effectors for use

11 nclude a plasmid construct for making custom TAL effectors and one for TAL effector fusions to additi

12 sign guidelines based on naturally occurring TAL effectors and their binding sites.

13 be used to predict binding sites of natural TAL effectors and to design novel synthetic DNA-binding

14 thy lines exhibiting gene activation by each TAL effector, and resistance to PXO99(A) , a PXO99(A) de

15 g the search space for off-targets of custom TAL effectors, and highlighting the potential of TAL eff

16 Transcription activator-like (TAL) effectors are encoded by plant-pathogenic bacteria

17 Transcription activator-like (TAL) effectors are repeat-containing proteins used by pl

18 Xa10 contains a binding element for the TAL effector AvrXa10 (EBEAvrXa10) in its promoter, and A

19 nas oryzae pv. oryzae (Xoo) that deliver the TAL effector AvrXa27.

20 TAL effectors bind DNA on the basis of a unique code tha

21 Bacterial transcription activator-like (TAL) effectors bind to effector-binding elements (EBEs)

22 gene could be broadened by adding different TAL effector binding elements (EBEs) to it.

23 arrays for desired targets and prediction of TAL effector binding sites, ranked by likelihood, in a g

24 e repeat variable di-residues that determine TAL effector binding specificity, and is independent of

25 lending support to the predictive models for TAL effector binding specificity.

26 e, by combining transcriptome profiling with TAL effector-binding element (EBE) prediction, we show t

27 Here, we demonstrate that TAL effectors can drive plant transcription from EBEs on

28 nt, and that sgRNA:Cas9 complexes and 18-mer TAL effectors can potentially tolerate 1-3 and 1-2 targe

29 tion domain of transcription activator-like (TAL) effectors can be combined with the nuclease domain

30 l mechanisms of resistance in plants against TAL effector-containing pathogens have given insights in

31 SWEET-targeting TAL effectors contribute broadly and non-tissue-specific

32 sucrose transporter genes were expressed in TAL effector-deficient X. oryzae strain X11-5A, and asse

33 TAL effectors delivered by phytopathogenic Xanthomonas s

34 es two host genes, CsLOB1 and CsSWEET1, in a TAL effector-dependent manner.

35 ion of Xa10, a transcription activator-like (TAL) effector-dependent R gene for resistance to bacteri

36 mputational model, Specificity Inference For TAL-Effector Design (SIFTED), to predict the DNA-binding

37 sucrose transporter, is induced by Avrb6, a TAL effector determining Xcm pathogenicity.

38 Xoc TAL effectors did not alter X11-5A virulence.

39 Artificially designed TAL effectors directed to sequences in the CsLOB1 promot

40 combinases fused to Cys2-His2 zinc-finger or TAL effector DNA-binding domains are a class of reagents

41 ions, the structure illustrates the basis of TAL effector-DNA recognition.

42 ent studies of transcription activator-like (TAL) effector domains fused to nucleases (TALENs) demons

43 Both TAL effectors drive expression of CsLOB1 and CsSWEET1 pr

44 enes oriented head to head, we show that the TAL effector drives expression from either EBE in the re

45 ectors enable custom-engineering of designer TAL effectors (dTALE) for gene activation.

46 Activation of GhSWEET10 by designer TAL effectors (dTALEs) restores virulence of Xcm avrb6 d

47 e modular nature and DNA recognition code of TAL effectors enable custom-engineering of designer TAL

48 Xoo TAL effectors enhanced X11-5A virulence on most varietie

49 tly evolved mechanism to recognize analogous TAL effector epitopes.

50 members of the transcription activator-like (TAL) effector family whose central repeat units dictate

51 members of the transcription activator-like (TAL) effector family.

52 members of the transcription activator-like (TAL) effector family.

53 effectors, and highlighting the potential of TAL effectors for probing fundamental aspects of plant t

54 sites for TAL effectors and customization of TAL effectors for use in DNA targeting, in particular as

55 Furthermore, we show that a native TAL effector from Xanthomonas oryzae pv. oryzicola drive

56 ession of multiple host genes using multiple TAL effectors from a single strain, and evidence support

57 f disease development in response to diverse TAL effectors from both X. oryzae pathovars.

58 sors in sweet orange, our data indicate that TAL effectors from X. citri target negative regulators o

59 Transcription activator-like (TAL) effectors from Xanthomonas citri subsp. malvacearum

60 Transcription activator-like (TAL) effectors from Xanthomonas species pathogens act as

61 for making custom TAL effectors and one for TAL effector fusions to additional proteins of interest.

62 ecies citri strain Xcc306, with the type III TAL effector gene pthA4 or with the distinct yet biologi

63 preventing disease by strains containing the TAL effector gene pthXo1, which directs robust expressio

64 lated gene using any one of a diverse set of TAL effector genes in the pathogen populations.

65 Three additional distinct TAL effector genes, pthA*, pthB, and pthC, also direct p

66 known type III transcription activator-like (TAL) effector genes for the characteristic pustule forma

67 dent on major transcription activation-like (TAL) effector genes, and correlates with reduced express

68 lack the N- and C-terminal regions, in which TAL effectors harbor their T3 and nuclear localization s

69 tremely modular DNA binding proteins such as TAL effectors, has generally proved to be quite challeng

70 ne with no corresponding naturally occurring TAL effector identified, conferred susceptibility to the

71 The roles of X. oryzae TAL effectors in diverse rice backgrounds, however, are

72 binding elements (EBEs) recognized by native TAL effectors in plants have been identified only on the

73 e prediction, and opens prospects for use of TAL effectors in research and biotechnology.

74 lity of X11-5A for characterizing individual TAL effectors in rice was established.

75 Rice resistance mechanisms against TAL effectors include polymorphisms that prevent effecto

76 DNA recognition by TAL effectors is mediated by tandem repeats, each 33 to

77 ng region of a transcription activator-like (TAL) effector is used to 'address' a site-specific megan

78 ly compatible with the Golden Gate TALEN and TAL Effector Kit 2.0, a widely used and efficient method

79 The modularity of DNA recognition by TAL effectors makes them important also as tools for gen

80 TAL effectors nuclear-localize in plants, where they bin

81 by the user to work with any TAL effector or TAL effector nuclease architecture.

82 om the FokI-derived zinc-finger nuclease and TAL effector nuclease platforms as the GIY-YIG domain al

83 box to include transcription activator-like (TAL) effector nuclease (TALEN)- and clustered regularly

84 Zinc-finger nucleases (ZFNs) and TAL effector nucleases (TALENs) have been shown to induc

85 domain of FokI restriction enzyme to produce TAL effector nucleases (TALENs) that, in pairs, bind adj

86 meganucleases, zinc-finger nucleases (ZFNs), TAL effector nucleases (TALENs), and CRISPR-associated s

87 , including zinc finger nucleases (ZFNs) and TAL effector nucleases (TALENs), have made it possible t

88 generating several cftr mutant alleles using TAL effector nucleases.

89 leases (ZFNs), transcription activator-like (TAL) effector nucleases (TALENs) and clustered regularly

90 Tal-effector nucleases (TALEN) and clustered regularly i

91 TAL-effector nucleases (TALENs) are attractive tools for

92 Tal-effector nucleases (TALENs) are engineered proteins

93 endonucleases, transcription activator-like [TAL] effector nucleases [TALENs], and homing endonucleas

94 A TAL effector-nucleotide binding code that links repeat t

95 developed a suite of web-based tools called TAL Effector-Nucleotide Targeter 2.0 that enables design

96 TAL effectors of plant pathogenic bacteria in the genus

97 We found previously that TAL effectors of the citrus canker pathogen Xanthomonas

98 ng T3-secreted transcription activator-like (TAL) effectors of plant pathogenic bacteria are encoded

99 Transcription activator-like (TAL) effectors of plant pathogenic bacteria contain a mo

100 Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to t

101 ters can be set by the user to work with any TAL effector or TAL effector nuclease architecture.

102 Xanthomonas transcription activator-like (TAL) effectors promote disease in plants by binding to a

103 we show that a transcription activator-like (TAL) effector released by endobacteria is an essential s

104 e Targeter 2.0 that enables design of custom TAL effector repeat arrays for desired targets and predi

105 Transcription activator-like (TAL) effector repeat domains fused to the LSD1 histone d

106 The result indicates that variations in the TAL effector repetitive domains are driven by selection

107 sm for protein-DNA recognition that explains TAL effector specificity, enables target site prediction

108 Our results reveal new modes of action for TAL effectors, suggesting the possibility of yet unrecog

109 AvrHah1 is a transcription activator-like (TAL) effector (TALE) in Xanthomonas gardneri that induce

110 Xoo TAL effectors that promote infection by activating SWEET

111 Incompatibility is associated with major TAL effectors that target the known alternative S genes

112 oduce numerous transcription activator-like (TAL) effectors that increase bacterial virulence by acti

113 rains or by engineering a synthetic designer TAL effector to boost SWEET gene expression.

114 The strong phenotypic similarity between the TAL effector-triggered resistance conferred by Xo1 and t

115 Several Xoc TAL effectors were tested in X11-5A on four rice varieti