ライフサイエンスコーパス: TALEN

1 TALEN treatment efficiently disrupted E6 and E7 oncogene

2 TALEN were shown to induce mutations in the target codin

3 TALEN-Agouti mRNAs injected into zygotes of brown FvB x

4 TALEN-mediated gene editing is a useful tool for dissect

5 TALEN-mediated gene targeting in avian PGCs is therefore

6 TALEN-modified CMV-specific T cells retained specific ki

7 TALEN-modified Ossabaw swine fetal fibroblasts were effe

8 TALENs targeting beta-catenin promoted endogenous HCC ca

9 TALENs thus appear to represent a highly facile platform

10 We used ICA to synthesize 20 TALENs of varying DNA target site length and tested thei

11 ew design guidelines for TALENs based on 205 TALENs tested, and established the scoring algorithm for

12 argeted changes in up to 33% (ZFNs) and 46% (TALENs) of blastocysts.

13 o combined both technologies and developed a TALEN-HDAdV hybrid vector, which significantly increased

14 Based on these findings, we engineered a TALEN variant that exhibits equal on-target cleavage act

15 s a mitochondrial protein whose absence in a TALEN-induced HEK293T knockout (KO) cell line leads to s

16 We evaluated the ability of a TALEN pair to target the Ae. aegypti kmo gene, whose pro

17 nd flexible tool for designing highly active TALENs for genome-editing applications.

18 n, and we engineered a pair of highly active TALENs that induce modification of 54% of human beta-glo

19 We also developed highly active TALENs to human gamma-globin, a pharmacologic target in

20 Additionally, TALEN proteins are added to the repertoire of custom-des

21 ocyte polyploidy might be protective against TALEN-induced loss of heterozygosity, and indeed Apc gen

22 Using CRISPR-Cas9 and TALEN targeted human pluripotent stem cell clones, we pe

23 management aid of reagent concentrations and TALEN formulation.

24 t genome editing in T cells using CRISPR and TALEN approaches.

25 mia (AML) cell lines, and present CRISPR and TALEN based methods for accomplishing complete gene knoc

26 cy gene targeting applications in CRISPR and TALEN compatible systems.

27 CRISPR and TALEN-based KO of Dck dramatically increased the IC(5)(0

28 e most widely used web tools for CRISPR- and TALEN-based genome editing.

29 ased program Mojo Hand for designing TAL and TALEN constructs for genome editing applications.

30 ed modularity and were active in TALE-TF and TALEN architectures.

31 describe a large-scale comparison of ZFN and TALEN mutagenicity in zebrafish.

32 sly been proposed to predict optimal ZFN and TALEN target sites did not predict mutagenicity in vivo.

33 The utility of CRISPR-Cas9 and TALENs for genome editing may be compromised by their of

34 a donor template along with CRISPR/Cas9 and TALENs respectively.

35 et binding of single-guide RNAs (sgRNAs) and TALENs.

36 CRISPR-Cas systems and TALENs can target desired genomic sites with high effici

37 ty in mammalian cells of custom TALE-TFs and TALENs using quantitative reverse-transcription PCR and

38 nd rapid construction of custom TALE-TFs and TALENs within 1 week and can be easily scaled up to cons

39 The modular architecture of ZFNs and TALENs allows for the rapid design of novel SSEs against

40 hlight the therapeutic potential of ZFNs and TALENs and discuss future prospects for the field, inclu

41 ZFNs and TALENs enable a broad range of genetic modifications by

42 The fact that ZFNs and TALENs have been used for genome modification of more th

43 Unlike ZFNs and TALENs that use protein motifs for DNA sequence recognit

44 characterize these cell lines, we anticipate TALEN-mediated genome editing of human cells becoming a

45 e efficiency, we administered adenoviral Apc TALENs and found that we could achieve a higher mutagene

46 A rate-limiting step in efficiently applying TALEN and CRISPR/Cas9 methods is the selection and desig

47 nome editing with designer nucleases such as TALEN and CRISPR/Cas enzymes has broad applications.

48 Sequence-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far been used t

49 We tested 48 FLASH-assembled TALEN pairs in a human cell-based EGFP reporter system a

50 SAPTA-based TALEN designs increased the average intracellular TALEN

51 d a significant negative correlation between TALEN mutagenicity and the number of CpG repeats in TALE

52 These results demonstrate that both TALEN and CRISPR-Cas9 are highly efficient tools for mod

53 we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can pro

54 Thus, cells transfected with both TALEN plasmids, a prerequisite for genomic editing, can

55 Both TALENs and CRISPR/Cas9 achieved gene targeting at simila

56 describe a mutant zebrafish line created by TALEN genome editing carrying a 23 bp deletion in gba1 (

57 re targeted to "spacer" sequences flanked by TALEN binding sites, larger deletions that extended beyo

58 onstructed an EYS-knockout zebrafish-line by TALEN technology which showed visual impairment at an ea

59 d a stable CERKL knockout zebrafish model by TALEN technology and a 7bp deletion in CERKL cDNA that c

60 C) technology and genome editing mediated by TALENs to generate isogenic subject-specific mutant and

61 16 of which were accessible and modified by TALENs in human cells.

62 The key features of direct mutagenesis by TALENs and oligodeoxynucleotides, minimal effort and hig

63 g the length of recognition DNA sequences by TALENs or ZFNs does not necessarily translate to a highe

64 fficient than cleavage of the same target by TALENs.

65 cific germline mutation in the mouse confirm TALEN mediated mutagenesis in the oocyte to be a viable

66 off-target cleavage activity and corroborate TALENs as a highly specific platform for future clinical

67 r of using transcriptome analysis and CRISPR/TALEN-based KOs to identify and verify genes associated

68 nd enables scientists to more rapidly deploy TALENs for genome editing applications.

69 DMY-TALENs resulted in indel mutations at the targeted loci

70 een 13-20 bp and T nucleotide preceding each TALEN binding site) in zebrafish.

71 veloped a robust TALENs system in which each TALEN plasmid also encodes a fluorescence protein.

72 in immunoprecipitation (ChIP), gene editing (TALEN) and chromosome conformation capture (3C) to local

73 Ns), transcription activator-like effectors (TALENs), and CRISPR, that have been widely used to manip

74 The most efficient TALEN was then selected for barley transformation.

75 Here, we describe a method that employs TALEN or CRISPR/Cas9-mediated knock-in of inducible degr

76 ly package full-length vector mRNAs encoding TALENs, but recombination during reverse transcription p

77 Here, we have engineered TALEN pairs targeting three human loci (CCR5, AAVS1 and

78 delivery system, we show that this enhanced TALEN toolkit has a high efficiency in inducing locus-sp

79 a simple transposon coselection strategy for TALEN-mediated gene modification in primary fibroblasts

80 enous gene-modification frequency of 39% for TALENs containing the repeat variable di-residue NK that

81 ines and developed new design guidelines for TALENs based on 205 TALENs tested, and established the s

82 target through linked protein domains (e.g. TALENs and zinc-finger nucleases).

83 orm is fully compatible with the Golden Gate TALEN and TAL Effector Kit 2.0, a widely used and effici

84 that the correction process did not generate TALEN-induced off targeting mutations by sequencing.

85 Using the beta-globin and gamma-globin TALENs, we generated cell lines that express GFP under t

86 Here, TALEN and CRISPR-Cas9, two versatile genome-editing tool

87 hat construction of one to two heterodimeric TALEN pairs for any given gene will, in most cases, enab

88 nduced by both homodimeric and heterodimeric TALENs are passed efficiently through the germline, in s

89 We also tested obligate heterodimeric TALENs and found that these nucleases induce mutations w

90 However, TALENs targeting Apc were not as efficient in inducing i

91 Here, we assembled improved TALENs targeting the DMY gene and generated XY(DMY-) mut

92 Our improved TALENs system can be applied to all cultured cells to ac

93 helix, or the side chains contacting DNA in TALEN-DNA complexes.

94 LE-R activity and up to 10-fold reduction in TALEN activity compared with target sequences containing

95 the presence of multiple sequence repeats in TALEN genes hampers the use of lentiviral vectors.

96 utagenicity and the number of CpG repeats in TALEN target sites, suggesting that target site methylat

97 entification of TAL binding sites for use in TALEN design.

98 ed that mutations in the FokI domain used in TALENs to generate obligate heterodimeric complexes subs

99 The cleavage activity of individual TALENs was first tested in vivo using a yeast-based, sin

100 While the initial TALEN-design guidelines are very useful, user-friendly t

101 designs increased the average intracellular TALEN monomer activity by >3-fold, and resulted in an av

102 ely target almost any desired genomic locus, TALEN is a technology that can revolutionize the entire

103 nd DNA relatively independently; (ii) longer TALENs are more tolerant of mismatches yet are more spec

104 lity to target essentially any sequence make TALENs the superior technology for targeted mutagenesis

105 In parallel experiments, we employed mito-TALENs to induce breaks in distinct loci of the mitochon

106 ytes using mitochondria-targeted TALEN (mito-TALENs).

107 s, genotypes, gene expressions, morpholinos, TALENs, CRISPRs, antibodies, anatomical structures, mode

108 DMY-nanos3UTR-TALENs induced mutations were passed through the germlin

109 Neither CRISPR/Cas9 nor TALEN increased BAC transgenesis.

110 Notably, TALEN expression was overall marked by a low cytotoxicit

111 nscription activator-like effector nuclease (TALEN) and clustered regularly interspaced short palindr

112 nscription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) can be engineered

113 nscription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing tec

114 nscription activator-like effector nuclease (TALEN) binding sites in the genome using a new software

115 nscription Activator-Like Effector Nuclease (TALEN) exhibited few off-target effects and a low level

116 nscription activator-like effector nuclease (TALEN) is an artificial sequence-specific endonuclease t

117 nscription activator-like effector nuclease (TALEN) messenger RNA.

118 nscription activator-like effector nuclease (TALEN) mRNA allows highly efficient multiplex gene editi

119 nscription activator-like effector nuclease (TALEN) nuclease to knockdown endogenous Dot1L in Xenopus

120 nscription activator-like effector nuclease (TALEN) technologies are powerful strategies for the gene

121 nscription activator-like effector nuclease (TALEN) technology to only disrupt the expression of Jalp

122 tion activator-like (TAL) effector nuclease (TALEN)- and clustered regularly interspaced short palind

123 nscription activator-like effector nuclease (TALEN)-based universal correction of HBB mutations in si

124 nscription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cel

125 nscription activator-like effector nuclease (TALEN)-mediated genome editing, we created a panel of is

126 nscription activator-like effector nuclease (TALEN)-mediated genome engineering.

127 Tal-effector nucleases (TALEN) and clustered regularly interspaced short palindr

128 scription activator-like effector nucleases (TALEN) mRNAs into mouse zygotes transferred into foster

129 scription activator-like effector nucleases (TALEN) were designed against beta-catenin (Ctnnb1) and a

130 nscription factors (TALE-TFs) and nucleases (TALENs) using a hierarchical ligation procedure.

131 ion activator-like effector-based nucleases (TALENs).

132 scription activator-like effector nucleases (TALENs) ameliorates the R14del-associated disease phenot

133 scription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palind

134 ion activator-like (TAL) effector nucleases (TALENs) and clustered regularly interspaced short palind

135 scription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palind

136 scription activator-like effector nucleases (TALENs) and CRISPR-Cas9.

137 riptional activator-like effector nucleases (TALENs) and CRISPR/CAS9 genome editing tools are used to

138 scription activator-like effector nucleases (TALENs) and synthetic oligodeoxynucleotides into one-cel

139 scription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short pa

140 scription activator-like effector nucleases (TALENs) are a new class of engineered nucleases that are

141 TAL-effector nucleases (TALENs) are attractive tools for sequence-specific genom

142 Tal-effector nucleases (TALENs) are engineered proteins that can stimulate preci

143 scription activator-like effector nucleases (TALENs) are powerful new research tools that enable targ

144 scription activator-like effector nucleases (TALENs) are programmable nucleases that join FokI endonu

145 scription activator-like effector nucleases (TALENs) can be designed to cleave chosen DNA sequences,

146 scription activator-like effector nucleases (TALENs) comprise a powerful class of tools that are rede

147 scription Activator-Like Effector Nucleases (TALENs) consist of a nuclease domain fused to a DNA bind

148 scription activator-like effector nucleases (TALENs) enable genome engineering in cell culture and ma

149 scription activator-like effector nucleases (TALENs) enable targeted mutagenesis in a variety of orga

150 scription activator-like effector nucleases (TALENs) for genome engineering, we demonstrate efficient

151 scription Activator-Like Effector Nucleases (TALENs) has provided a new experimental partnership for

152 scription activator-like effector nucleases (TALENs) have become a powerful tool for genome editing d

153 nucleases (ZFNs) and TAL effector nucleases (TALENs) have been shown to induce targeted mutations, bu

154 scription activator-like effector nucleases (TALENs) have emerged as a highly effective tool for geno

155 scription activator-like effector nucleases (TALENs) induces apoptosis, inhibits growth, and reduces

156 scription activator-like effector nucleases (TALENs) is a valuable tool for precise, site-specific ge

157 scription activator-like effector nucleases (TALENs) or clustered, regularly interspaced, short palin

158 scription activator-like effector nucleases (TALENs) or RNA-guided clustered regularly interspaced sh

159 scription activator-like effector nucleases (TALENs) or zinc-finger nucleases (ZFNs).

160 scription activator-like effector nucleases (TALENs) provide a powerful new approach for targeted zeb

161 scription activator-like effector nucleases (TALENs) provide powerful platforms for genome editing in

162 scription activator-like effector nucleases (TALENs) represent a promising approach for targeted knoc

163 scription activator-like effector nucleases (TALENs) that are based on bacterial TALEs fused to the F

164 scription activator-like effector nucleases (TALENs) that recognize two adjacent unique DNA sequences

165 scription activator-like effector nucleases (TALENs) to completely eradicate all LEDGF/p75 expression

166 scription activator-like effector nucleases (TALENs) to localize to mitochondria and cleave different

167 scription activator-like effector nucleases (TALENs) to target interleukin-2 receptor subunit gamma (

168 scription activator-like effector nucleases (TALENs) with DNA oligonucleotides (ODNs).

169 er nucleases (ZFNs), TAL effector nucleases (TALENs), and CRISPR-associated system 9 (Cas9) proteins,

170 scription activator-like effector nucleases (TALENs), and CRISPR/Cas RNA-guided nucleases (RGNs).

171 scription activator-like effector nucleases (TALENs), completely removes ORN sensitivity to bombykol

172 nucleases (ZFNs) and TAL effector nucleases (TALENs), have made it possible to precisely modify plant

173 scription activator-like effector nucleases (TALENs), relies on safe and effective means of deliverin

174 cription Activator-Like Effectors Nucleases (TALENs) and Cas9-gRNA allows genetic modifications to be

175 cription activator-like effectors nucleases (TALENs) with broadly improved DNA cleavage specificity,

176 anscription factors (TALE-TFs) or nucleases (TALENs), enabling precise gene manipulations.

177 TALE nucleases (TALENs) have been used with great success in a number of

178 In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 (vasa

179 LE recombinases (TALE-R) and TALE nucleases (TALENs) with each DNA base at this position.

180 finger nucleases (ZFNs) and Tale nucleases (TALENs), and has enabled us to insert a 15-kb inducible

181 e (TAL) effector domains fused to nucleases (TALENs) demonstrate enormous potential for genome editin

182 ion activator-like [TAL] effector nucleases [TALENs], and homing endonucleases [also called meganucle

183 NA-editing nucleases (Zinc Finger Nucleases, TALENs, CRISPR/Cas systems) has given scientists the abi

184 report that programmable nucleases-TALENs and CRISPR/Cas9-can safely and efficiently correc

185 ese results demonstrate the applicability of TALEN-mediated genome editing to a scalable process, whi

186 The assembly of TALEN constructs, is also simplified by using the TAL-si

187 Zygotic coinjection of TALEN mRNAs directed to the Agouti, miR-205, and the Arf

188 tabase searches, we designed a collection of TALEN constructs to knockout 88 human genes that are ass

189 argeting vector influenced the efficiency of TALEN-mediated homologous recombination.

190 We found little evidence of TALEN off-target effects, but each clonal line neverthel

191 ng targeted mutagenesis by microinjection of TALEN mRNA within the mouse oocyte.

192 report detailed genotypes and phenotypes of TALEN-edited MECP2 mutant cynomolgus monkeys serving as

193 Our results establish the robustness of TALEN technology and demonstrate that FLASH facilitates

194 The primary advantage of TALENs over other sequence-specific nucleases, namely zi

195 in hPSCs with a focus on the application of TALENs and CRISPR/Cas9.

196 effective method for large-scale assembly of TALENs.

197 Effective design of TALENs requires a combination of selecting appropriate g

198 m and demonstrate controlled co-injection of TALENs or CRISPR/Cas9 together with donor template into

199 tured cells, two plasmids encoding a pair of TALENs are co-transfected, followed by limited dilution

200 A pair of TALENs binds to two DNA recognition sites separated by a

201 ulti-copy gene family using only one pair of TALENs.

202 iting approaches, we test the specificity of TALENs by disrupting the H2A.B multi-copy gene family us

203 Combining the high specificity of TALENs with efficient lentiviral gene delivery should ad

204 that one subunit of ZFNs and one subunit of TALENs can form a pair of hybrid nucleases with expanded

205 smission data from CRISPR/Cas9 with those of TALENs and ZFNs and shows that efficiency of CRISPR/Cas9

206 entiviral-vector RNA would allow transfer of TALENs as mRNA.

207 We report here the use of TALENs to rapidly and efficiently generate mutant allele

208 useful, user-friendly tools defining optimal TALEN designs for robust genome editing need to be devel

209 sites, facilitating the selection of optimal TALEN pairs based on predicted activity.

210 eering technologies-piggyBac, CRISPR/Cas9 or TALEN.

211 iRNAs were not detected from CRISPR/Cas9- or TALEN-induced DSBs within the examined endogenous genes

212 Here, we used CRISPR/Cas9- or TALEN-triggered DSBs to characterize diRNAs in Arabidops

213 ate that antibody-mediated neutralization or TALEN-mediated genetic inactivation of GMCSF in CAR T-ce

214 HTGTS with different Cas9:sgRNA or TALEN nucleases revealed off-target hotspot numbers for

215 ntegrases at the H11 locus by spontaneous or TALEN-assisted homologous recombination.

216 for engineering and application of TALE- or TALEN-based systems for genome editing and regulation.

217 ondria-targeted restriction endonucleases or TALENs.

218 ins, which are intrinsically cell-permeable; TALEN proteins, which can be internalized via conjugatio

219 rest, SAPTA gives a ranked list of potential TALEN target sites, facilitating the selection of optima

220 blished the scoring algorithm for predicting TALEN activity (SAPTA) as a new online design tool.

221 ssive DNA-binding energy can lead to reduced TALEN specificity in cells.

222 Remarkably, TALENs or CRISPR-Cas9-mediated double-strand DNA breaks

223 Here, we report TALEN-mediated genome editing of the IL2RG locus.

224 We have developed a robust TALENs system in which each TALEN plasmid also encodes a

225 be designed to cleave chosen DNA sequences, TALENs have activity against related off-target sequence

226 Here we report the assembly of several TALENs for a specific genomic locus in barley.

227 gene in the first coding exon with a single TALEN pair yielded trace LEDGF/p75 levels that were viro

228 DDX4 locus were also created using a single TALEN pair.

229 Coselection after treatment with a single TALEN-pair enabled isolation of colonies with mono- and

230 on may explain the poor mutagenicity of some TALENs in vivo.

231 to the advent of the versatile and specific TALEN systems, and most recently the highly accessible C

232 The rational design of a CCR5-specific TALEN pair decreased off-target activity at the closely

233 ciple, we transferred CCR5- and TCR-specific TALEN pairs for efficient targeted genome editing and ab

234 ff-target sites for CCR5- and AAVS1-specific TALENs.

235 ation assay to demonstrate that HIV-specific TALENs can reduce the fraction of reactivatable virus in

236 and (4) output files designed for subsequent TALEN construction using the Golden Gate assembly method

237 Here we report successful TALEN-mediated mutagenesis of an X-linked, Rett syndrome

238 ludes engineered zinc finger proteins, TALEs/TALENs, and the CRISPR/Cas9 system as nucleases for geno

239 ammalian oocytes using mitochondria-targeted TALEN (mito-TALENs).

240 Mitochondria-targeted TALEN (mitoTALEN) expression led to permanent reductions

241 ct cervical application of HPV16-E7-targeted TALENs effectively mutated the E7 oncogene, reduced vira

242 Microinjection of MECP2-targeting TALEN plasmids into rhesus and cynomolgus zygotes leads

243 ) and found that, with beta-globin-targeting TALENs, similar levels of on- and off-target activity in

244 ese methods to identify, construct, and test TALENs that were used with HDR donors in hESCs to genera

245 All 14 tested TALEN pairs (100%) introduced small insertions and delet

246 We conclude that TALEN-mediated mutagenesis can be an effective tool for

247 We observed that TALEN and ZFN have a reduced capability of secondary hom

248 rescence in situ hybridization revealed that TALEN pairs targeting the Agouti locus induced site-dire

249 Here we show that TALEN of dmrt1 efficiently induced mutations of this gen

250 s of the resulting transformants showed that TALEN-induced double strand breaks led to the introducti

251 We conclude that TALENs are highly active in the Ae. aegypti germline, an

252 Using deep sequencing, we found that TALENs are significantly more likely to be mutagenic and

253 ed nucleotides, raising the possibility that TALENs might be used to perform allele-specific gene dis

254 We show that TALENs do display a high level of specificity since no o

255 We show that TALENs mediate homology-directed repair of the DDX4 locu

256 Recent work has shown that TALENs can induce mutations in endogenous zebrafish gene

257 her, the results from our study suggest that TALENs have potential as a therapeutic strategy for HPV

258 The TALEN pairs were designed to induce double-strand DNA br

259 oftware program, TALENSeek, (2) assemble the TALEN genes by combining golden gate cloning with modifi

260 s, larger deletions that extended beyond the TALEN-binding sequences were also detected and were simi

261 Furthermore, endowing the TALEN-engineered cells with a CD19 CAR led to efficient

262 en made possible with the development of the TALEN and CRISPR/Cas9 methods.

263 Here we report the utilization of the TALEN and CRISPR/Cas9 systems to induce targeted mutatio

264 To illustrate the utility of the TALEN-mediated knockout technique, 6 individual genes (T

265 ts from the FLASH protocol, and (3) test the TALEN pairs in an amplification-based HDR assay that is

266 We show that the TALEN was extremely efficient in mutating Dot1L when exp

267 two newly developed gene-editing tools, the TALEN and CRISPR/Cas systems, we have developed a genome

268 ced the first H2A.B knockout mouse using the TALEN approach.

269 The TALENs showed comparable activity to benchmark ZFNs, wit

270 We asked whether these TALENs could create targeted somatic mutations after hyd

271 We use this TALEN-mediated editing approach to develop a process for

272 id sequences of their DNA-binding domains to TALEN nucleotide targets.

273 We transfected TALENs along with a targeting vector into Jurkat cells,

274 Coselection after treatment with two TALEN-pairs directed against the same chromosome enabled

275 MiPs from dystrophic dogs that had undergone TALEN-mediated correction of the MD-associated mutation

276 To better understand TALEN specificity, we profiled 30 unique TALENs with dif

277 and TALEN specificity, we profiled 30 unique TALENs with different target sites, array length and dom

278 With this updated TALEN system, we successfully used single-stranded DNA o

279 Here, we used TALEN and CRISPR/Cas-mediated gene editing and hPSC-dire

280 nd reduce CPI-associated toxicities, we used TALEN technology to render tumor-reactive T cells resist

281 We used TALEN-mediated genome editing in fertilized mouse oocyte

282 We used TALEN-technology to knock out the nuclear gene encoding

283 erimentally useful human cell lines, we used TALENs to definitively eradicate LEDGF/p75 by deleting e

284 We used TALENs to generate five zebrafish abcd1 mutant allele li

285 cking all four zebrafish Mesp genes by using TALEN-mediated genome editing.

286 e generated a zyx mutant in Drosophila using TALEN endonucleases and used this to show that Zyx antag

287 s in pig, goat, and cattle fibroblasts using TALEN mRNA and oligonucleotide transfection with efficie

288 labeled vimentin with an mEmerald tag using TALEN genome editing.

289 nerated genetic mosaic adult zebrafish using TALEN genome editing and demonstrate somatic inactivatio

290 Their application using TALENs and CRISPR/CAS9 based genomic editing approach is

291 brafish (zap70(y442)) that was created using TALENs.

292 ptimized procedure for genomic editing using TALENs is also presented.

293 In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the

294 Sequence-verified TALENs or TALE-TF plasmids targeting 17 bp target sequen

295 eered VF mucosae from hiPSC, transfected via TALEN constructs for green fluorescent protein, that mim

296 11-33% of 1-cell stage embryos injected with TALEN mRNAs targeting rb1 exon 2 or 3 develop tumors beg

297 targeting occurred at an average of 14% with TALENs and 33% with CRISPR.

298 ry fetal fibroblasts, which were edited with TALENs and single-strand oligonucleotide to introduce a

299 Here we used designer nucleases (ZFNs, TALENs, and CRISPR/Cas9) to introduce DSBs on two chromo

300 We observed improved efficiency using ZFNs, TALENs, two CRISPR/Cas9, and CRISPR/Cas9 nickase to targ