ライフサイエンスコーパス: TCR-beta

1 TCR beta-chain CDR3-length distribution analysis using P

2 TCR beta-chain contacts are mostly through the variable

3 TCR beta-chain repertoires were polyclonal in infants.

4 TCR beta-chain V region gene diversity was determined by

5 TCR-beta and CDR-beta gene usage of single islet-infiltr

6 TCR-beta gene recombination and allelic exclusion were n

7 a similar increase was not observed in GT(0)/TCR-beta(0) mice, and the titer of alphaGal-specific Abs

8 evel of alphaGal-specific serum Abs in GT(0)/TCR-beta(0) mice.

9 (0)) to generate double-knockout mice (GT(0)/TCR-beta(0)).

10 While GT(0)/TCR-beta+ mice exhibited an age-dependent increase in th

11 Treatment of GT(0)/TCR-beta+ mice with anti-CD40L Abs before immunization w

12 erum titer of alphaGal-specific Abs in GT(0)/TCR-beta+ mice, but had no effect on the level of alphaG

13 ignificantly lower than in age-matched GT(0)/TCR-beta+ mice.

14 cell pool drastically contracted to 200,000 TCR beta-chains.

15 tely flanking intron sequences of a Vbeta8.1 TCR-beta gene.

16 -J alpha 281 TCR, flow cytometry of NK1.1(+) TCR beta(+) cells, and analysis of cytokine production b

17 esults in a significant reduction of NK1.1(+)TCR-beta(+) and CD1d tetramer-positive cells, consistent

18 chronic collagen-induced arthritis in DBA/1-TCR-beta transgenic mice, as well as collagen-induced ar

19 polyclonal V beta 8, V beta 7, and V beta 2 TCR beta chains.

20 Finally, and of importance, IL-2Ralpha(-/-) TCR-beta(-/-) mice had abrogation of liver and colon pat

21 assessed by the DNA sequence analysis of 50 TCR beta clones obtained by rapid amplification of cDNA

22 mensional structure of the complex between a TCR beta chain (mouse V beta8.2) and the SAG staphylococ

23 (TCR alpha) chains in mice transgenic for a TCR beta chain has allowed us to demonstrate a central r

24 ng a murine fasL cDNA under the control of a TCR beta-chain enhancer minigene.

25 e diseases through transient expression of a TCR beta-chain.

26 cule on an antigen- presenting cell and to a TCR beta-chain, thereby causing activation of the T cell

27 t, complementation of Rag-2(-/)- mice with a TCR beta transgene restored ICN1-induced DP development

28 is of T cell receptor (TCR) repertoires in a TCR-beta transgenic model.

29 lopment is rescued through transduction of a TCR-beta transgene.

30 s TCR-alpha chain even when it paired with a TCR-beta chain of irrelevant specificity.

31 le T-lineage cell that had stable bi-allelic TCR beta rearrangements.

32 pecific gene enhancers, including TCR-alpha, TCR-beta, and CD4.

33 mplexes comprise a disulfide-linked pT alpha-TCR-beta heterodimer associated not only with CD3-gamma

34 e Vbeta8.1/8.2 TCR that is predominant among TCR-beta+ T cells.

35 In this study, we analyze TCR-beta recombination and cell cycle status with respec

36 rangement and allelic exclusion, we analyzed TCR beta chromatin structure in double negative (DN) thy

37 We analyzed TCR beta-chain and alpha-chain genes expressed by these

38 rs encoding the HIV-1-specific TCR alpha and TCR beta chains cloned from a CTL clone specific for an

39 3 proteins are expressed before pT alpha and TCR beta-chains in prothymocytes and are expressed intra

40 lex may function independent of pT alpha and TCR beta.

41 to accommodate juxtaposition of CD3gamma and TCR beta ectodomains and foster quaternary change that c

42 situated underneath the TCR alpha-chain and TCR beta-chain, respectively.

43 Both TCR alpha-chains and TCR beta-chains made contact with the CD1d molecule with

44 HSV-specific lesional CD4(+) cell clones and TCR beta -variable (TCRBV) sequencing confirmed that the

45 ns displayed differential MR1 dependency and TCR beta-chain bias, consistent with possible divergent

46 uction, homeostatic T cell proliferation and TCR beta-chain diversity in young (approximately 25 year

47 unique phenotypic traits of CD8(+) TILs and TCR beta chain (TCRbeta) clonotypic frequency in melanom

48 enic mice devoid of endogenous TCR-alpha and TCR-beta chains.

49 method for expressing defined TCR-alpha and TCR-beta proteins from a single 2A peptide-linked multic

50 hus, we examined nucleotide loss from Ig and TCR-beta coding joints in mice lacking p53.

51 affinity purified on anti-TCR-alpha and anti-TCR-beta mAb columns had identical CS-protective activit

52 +) T cells transcribe Il2 within 6 h of anti-TCR-beta plus anti-CD28 stimulation (early phase).

53 These cells function redundantly, as TCR-beta(-/-) and TCR-delta(-/-) mice were both resistan

54 B6 TCR beta-chain knockout (KO) recipients of a myelin olig

55 nscripts increased the levels of PTC-bearing TCR-beta transcripts in the nuclear fraction of cells.

56 productive "in-frame" T cell receptor beta (TCR beta), immunoglobulin (Ig) heavy (H) or Ig light (L)

57 n in-frame T cell receptor for antigen beta (TCR-beta) gene rearrangement will continue to mature.

58 The random nature of T-cell receptor-beta (TCR-beta) recombination needed to generate immunological

59 53(-/-) mice die of T-cell receptor-beta(-) (TCR-beta(-)) thymic lymphomas with translocations and ot

60 l receptor (TCR) repertoire in terms of both TCR-beta usage and clonal composition.

61 es and criteria for lineage choice when both TCR-beta and gammadelta-TCR are simultaneously expressed

62 human and mouse MAIT cells expressed a broad TCR-beta repertoire, and although the majority of human

63 s recognizing TCR Vbeta chain, as well as by TCR beta gene rearrangements.

64 ression and facilitates signaling from a CD3-TCR beta complex.

65 expressing IFN-gamma and of NKp46/CD335(+), TCR-beta(+) cells expressing IL-13.

66 h) cells stained positive for CD2, CD3, CD8, TCR beta-chain, and NK1.1 but did not express the B cell

67 with surface determinants for CD3, CD4, CD8, TCR-beta, or TCR-delta molecules.

68 cDNA-TCR beta-chain libraries were sequenced from 2 million p

69 erantigens stimulate T cells bearing certain TCR beta-chain variable regions when bound to MHC II mol

70 -cell-deficient (T-cell receptor beta chain [TCR-beta] -/- or TCR-betaxdelta -/-) mice was shown to e

71 Using clonotypic TCR beta-chain length and sequence analysis we confirmed

72 n a buried hydroxymethyl that forms a common TCR beta-chain V region variant.

73 by directly targeting the murinized constant TCR beta domain (TCRmu) with a zirconium-89 ((89)Zr)-lab

74 e considered to be associated with defective TCR beta-chain rearrangement.

75 mma delta cells, newborn TCR beta-deficient (TCR beta(-/-)) thymi were grafted to IL-7(-/-) mice.

76 age clearance in T-cell receptor beta/delta (TCR-beta/delta) knockout (KO) and IgH-mu KO mice, respec

77 ntained at approximately 2 x 10(7) different TCR beta-chains.

78 hannel catfish revealed distinctly different TCR beta rearrangements.

79 ower bound for the total number of different TCR beta (TCRB) sequences in human repertoires.

80 However, these cells have diverse TCR beta-chains, including Vbeta8, Vbeta7, and Vbeta2 in

81 d the residues in the CDR3 region of the DN1 TCR beta-chain that were predicted to project between th

82 cell clones expressing the in vivo dominant TCR beta-chain sequences were identified in three patien

83 the rearrangement potential of Dbeta2 during TCR beta locus assembly.

84 xpress both transgene derived and endogenous TCR beta chains.

85 ) that promoted the disruption of endogenous TCR beta- and alpha-chain genes.

86 drives gene rearrangement at the endogenous TCR beta locus and results in the appearance of Vbeta5(-

87 ific transgenic mice lacking only endogenous TCR-beta chains also developed EAE, suggesting that in T

88 s, we designed a system where the endogenous TCR-beta is knocked out from the recipient cells using c

89 , cells with high protective activity escape TCR-beta chain allelic exclusion.

90 They exhibit TCR-beta gene loci in germline configuration and show lo

91 hese IEL from lck-/-fyn-/- animals exhibited TCR beta-gene rearrangement.

92 On introduction of exogenous TCR-beta chains, but not of TCR-alpha chains, assembly a

93 V CTL clones were all Vbeta13+ and expressed TCR beta-chains with highly homologous complementarity-d

94 th impaired Th2 cytokines, IL-17A expressing TCR beta (+) T cells were increased, while IL-22 express

95 HC and how they stimulate T cells expressing TCR beta chains from a number of different families, res

96 negative selection of thymocytes expressing TCR beta-chains reactive against several retroviral supe

97 ed by one or two clones typically expressing TCR beta-chain variable TRBV-15.

98 ve (DN) thymocytes, which are permissive for TCR beta recombination, and in double positive (DP) thym

99 This finding indicates a role for TCR beta in defining natural killer T cell specificity,

100 that Rho acts as an intracellular switch for TCR beta selection, the critical thymic-differentiation

101 tes exhibited a lower rate of mortality from TCR-beta(-) tumors, which harbored significantly elevate

102 Assembly of a functional TCR beta-chain gene triggers feedback inhibition of V(be

103 l cells just before selection for functional TCR beta-chain expression.

104 l features of this interaction, we generated TCR beta-chain transgenic mice using a TCR derived from

105 s can be explained predominantly by germline TCR-beta locus factors and not TCR-beta allelic or HLA e

106 In this study, we characterized the global TCR beta-chain profile in human T cells isolated from pl

107 e Valpha24 Vbeta11 clones were shown to have TCR-beta CDR3 diversity and express the natural killer (

108 es an entirely different, more heterogeneous TCR beta-chain repertoire that fails to recognize specif

109 n expanded rapidly after OPC, exhibited high TCR-beta clonal diversity, and was absent in Rag1(-/-),

110 Overall, homologous TCR beta- and alpha-chains showed identical V regions an

111 s, defined as those that expressed identical TCR beta-chain amino acid sequences and recurred in mult

112 In TCR beta locus, rearrangement initiates at two D-J casse

113 In TCR beta mice tetramer-positive thymocytes are detectabl

114 esponding to NP were dramatically altered in TCR beta(-/-) mice.

115 nfirmed severe inflammation in Wt but not in TCR-beta x delta(-/-) mice.

116 urs in gammadelta-selected DN3 thymocytes in TCR-beta-/- mice and in IL2-GFP transgenic reporter mice

117 ties that are likely conferred by individual TCR beta-chain rearrangements.

118 Interestingly, cross-analysis of individual TCR beta repertoires revealed a set >10,000 of the most

119 toire, with a median frequency of individual TCR beta-chain sequences of 1 in 2.4 x 10(7) CD4 T cells

120 habeta-lineage differentiation by inhibiting TCR-beta formation.

121 reduced amounts of surface and intracellular TCR-beta protein and decreased levels of tcrbeta transcr

122 ta selection, concomitant with intracellular TCR-beta expression.

123 Individual residues within the MAIT TCR beta chain were dispensable for the interaction with

124 In RA patients, the median TCR beta-chain frequency was increased 10-fold, indicati

125 ase manifestations in T cell-deficient mice (TCR-beta x delta(-/-)), although it resulted in bacterem

126 ce were crossed with TCR-beta knockout mice (TCR-beta(0)) to generate double-knockout mice (GT(0)/TCR

127 While pT alpha(a) acts to retain most TCR beta-chains intracellularly, pT alpha(b) permits hig

128 eature was also observed for human and mouse TCR beta chains, although the alpha and beta chain V-gen

129 By using specific mAbs to mouse TCR-beta (H57) and CD3epsilon (2C11) subunits, we herein

130 hat contribute to the activity of the murine TCR beta enhancer in mature and immature T cell lines.

131 m of the D beta 1 gene segment in the murine TCR beta locus was deleted to assess its role in control

132 evelopment of TCR gamma delta cells, newborn TCR beta-deficient (TCR beta(-/-)) thymi were grafted to

133 These findings indicate that NKT TCR beta-chain diversity results in differential and non

134 No TCR beta chain restriction was found.

135 n thymocytes, resulting in a burst of normal TCR beta and delta rearrangements.

136 y by germline TCR-beta locus factors and not TCR-beta allelic or HLA effects.

137 revision requires the rearrangement of novel TCR beta-chain genes and depends on recombinase-activati

138 Structural analysis of TCR beta-chain usage in such patients demonstrated a jun

139 R repertoires focus solely on an analysis of TCR beta-chains, rather than the combined TCRalphabeta h

140 J12 in humans), which pairs with an array of TCR beta-chains.

141 To investigate chromatin control of TCR beta rearrangement and allelic exclusion, we analyze

142 ue was used to determine the distribution of TCR beta-chain V region sequences expressed in the trans

143 ween beta selection and allelic exclusion of TCR beta.

144 promoter that drives germline expression of TCR beta gene segments in vivo.

145 survival of thymocytes lacking expression of TCR beta, showing hallmarks of hyperactive Notch signali

146 hymocytes are selected for the expression of TCR beta-chains with shorter CDR3 at the double-positive

147 lity to proliferate, which is independent of TCR beta-chain CDR3 sequence or precursor frequency.

148 subpopulations, known to be the location of TCR beta-chain rearrangement.

149 entarity-determining region 3 (CDR3) loop of TCR beta chains.

150 synovial fluid was analyzed using a panel of TCR beta variable region-specific monoclonal antibodies.

151 e deep and precisely normalized profiling of TCR beta repertoires in 39 healthy donors aged 6-90 y.

152 cell clone had functional rearrangements of TCR beta-chain genes using the Vbeta120.la and Jbeta1.1

153 Reconstitution of TCR beta-chain KO mice with wild-type spleen cells halte

154 The variable region of TCR beta-chain (Vbeta) gene usage was determined by fluo

155 diate Vav-1 action as critical regulators of TCR beta selection.

156 otpads with reovirus, and the repertoires of TCR beta-chains expressed on virus-specific CD8(+) T cel

157 Then, we applied CDR3 spectrotyping of TCR beta-chain to assess the clonality of T cells at dif

158 vitro studies to be located at the stages of TCR beta-chain rearrangement.

159 Furthermore, deep-sequencing analysis of TCR-beta (TRB) and TCR-alpha (TRA) rearrangements of CD3

160 stant alpha domains, paired with an array of TCR-beta chains.

161 beta region, are involved in the control of TCR-beta allelic exclusion.

162 tion of immune cells into the spinal cord of TCR-beta x delta(-/-) mice was reduced and the resident

163 mice with p53 deletion in thymocytes die of TCR-beta(-) tumors containing Tcralpha/delta translocati

164 ositive thymocytes, diminished expression of TCR-beta, and increased expression of CD25, suggesting a

165 We further show that the down-modulation of TCR-beta expression requires contact between S.

166 ibition of NMD induces premature shut-off of TCR-beta rearrangement.

167 D4(-)CD8(-) thymocytes during the process of TCR-beta chain rearrangement by a recombinase-independen

168 third complementarity-determining region) of TCR-beta.

169 We found that the down-regulation of TCR-beta transcripts in response to nonsense codons requ

170 monkeys exhibited the dominant responses of TCR-beta complementarity-determining region 3-restricted

171 sive immune profiling and deep sequencing of TCR-beta V regions, two subsets of cTregs, based on expr

172 MS2-3C8 that indicate long-range effects on TCR beta chain conformation and dynamics.

173 dily detectable in normal T1 cells, but only TCR-beta intermediates were detected in IL-7R-/- T1 cell

174 f secondary infection, we observed that only TCR-beta deficiency or simultaneous neutralization of IL

175 ymocytes die at later ages to TCR-beta(-) or TCR-beta(+) thymic lymphomas containing a similar patter

176 In contrast, CD4-/- or TCR-beta-/- mice develop polymicrobial sepsis and end-or

177 sets of CD8(+) cells expressing a particular TCR beta-chain variable region were more commonly identi

178 specificity of different SAGs for particular TCR beta chains, and for the observed influence of the T

179 on proliferation of T cells with particular TCR beta-chains, which occurs upon recognition of virall

180 o imply that the polymorphism of the porcine TCR beta segments is similar to that found in human.

181 Pak2 was required for pre-TCR beta-selection and positive selection.

182 lon treatment that mimics the process of pre-TCR beta-selection of thymocytes to the double positive

183 found that expression of these preassembled TCR beta-chains did not downregulate recombinational acc

184 Public TCR beta-chain sequences were identified across differen

185 biased and frequently dominated by a public TCR beta-chain encoded by the variable gene segment TRBV

186 et >10,000 of the most representative public TCR beta clonotypes, whose abundance among the top 100,0

187 an and murine T cells have shown that public TCR beta-chain rearrangements can dominate the Ag-specif

188 g three approaches: (a) in vivo quantitative TCR beta chain V segment and complementarity determining

189 tion of a pre-TCR that includes a rearranged TCR beta-chain and the pre-TCR alpha-chain.

190 -TCR alpha-chain (pT alpha) and a rearranged TCR beta-chain assemble to form the pre-TCR that control

191 selection, mice transgenic for a rearranged TCR beta-chain derived from conventional alphabeta T lym

192 expressing diverse, endogenously rearranged TCR beta chains.

193 requires a signal from the newly rearranged TCR beta chain.

194 Dbeta1-like sequence in numerous rearranged TCR beta cDNA suggests the existence of two D-J clusters

195 Selection of a productively rearranged TCR beta-chain is the first stage in the process and occ

196 In mice expressing single, rearranged TCR beta-chains, individual mutation of amino acids in t

197 Thus, in vivo expression of the rearranged TCR beta-chain from a thymus-derived NK1.1+ Valpha14+ T

198 ed by the introduction of a fully rearranged TCR-beta transgene that precludes generation of out-of-f

199 in the absence of a functionally rearranged TCR-beta allele.

200 CTL receptor TCR beta-chain variable gene subfamilies were polyclonal

201 toxin B (SEB) mutants to soluble recombinant TCR beta chain and to the human MHC class II molecule HL

202 contact occurs, a factor capable of reducing TCR-beta expression is secreted.

203 he magnitude of the CD8(+) T cell responses, TCR beta-chain repertoires did not significantly differ

204 eukemic cells from the twins shared the same TCR beta gene rearrangement with an identical 11 bp N re

205 at >90% of the hybridomas expressed the same TCR beta-chain variable region (V(beta)10), and sequenci

206 sponding cells were determined by sequencing TCR beta chain variable (TCRBV) genes.

207 Additionally, the sequences of several TCR beta-chain CDR3 regions were homologous to TCR beta-

208 We found that CTL clones sharing TCR beta-chains exhibited disparate recognition patterns

209 selection of thymocytes that express shorter TCR beta-chain complementarity-determining region 3 (CDR

210 that most alphabeta T cells express a single TCR beta chain and most B cells express single IgH and I

211 f the T cell repertoire to usage of a single TCR beta-chain, Vbeta11, implying selection by Ag.

212 pha(b) permits higher levels of cell surface TCR beta expression and facilitates signaling from a CD3

213 In CD4 thymocytes expressing a fixed Tg TCR beta-chain, the associated TCRalpha sequences in wil

214 T cells lacking surface expression of the Tg TCR beta chain and expressing diverse, endogenously rear

215 We demonstrate that TCR beta diversity per 10(6) T cells decreases roughly l

216 In this study, we demonstrate that TCR beta-chain composition can dramatically influence li

217 ne is likely to be Tcrb-V13, indicating that TCR beta-chain usage is a determinant of susceptibility

218 We also found that TCR-beta (TRB) V usage was highly restricted among 70-kD

219 Our data suggest that TCR-beta selection is not affected in p53-deficient, V(D

220 The TCR beta-chain gene enhancer activates transcription and

221 The TCR beta-chain repertoire of memory T cells specific for

222 xtremely poor shape complementarity, and the TCR beta chain complementarity-determining region 3 (CDR

223 omplexes with MHC class II molecules and the TCR beta chain, provide a framework for understanding th

224 RAG-/- mice, DJ and VDJ recombination at the TCR beta locus was functional, and normal numbers of NK

225 atures important for interaction between the TCR beta-chain and the peptide/MHC complex, we immunized

226 strated a TCR motif in humans defined by the TCR beta-chain variable gene 4-1 (TRBV4-1) region.

227 r emphasis on recognition of the pMHC by the TCR beta-chain, evinced by an increase in shape compleme

228 ehensive analysis of genes that comprise the TCR beta variable gene (TCRBV) repertoire of the common

229 To this end, we determined the TCR beta chain (Vbeta) usage of CD8(+) T cells from thre

230 In this study, we examined the TCR beta-chain (TRB) diversity of the CD8(+) T cell resp

231 pre-existing T cells commonly expressing the TCR beta chain variable region (TCR BV) 20 and increased

232 anscription in most cell types; however, the TCR beta enhancer (E beta) stimulates PD beta function s

233 induces long-range allosteric changes in the TCR beta chain at conserved sites in both representative

234 o define specific structural features in the TCR beta chain that are important for the recognition of

235 Activation of the TCR beta (Tcrb) locus in committed thymocytes is a criti

236 dels for the generation and selection of the TCR beta chain (TRB) from sequenced repertoires of 651 i

237 arison, mice with targeted disruption of the TCR beta chain and expressing no alpha beta TCR(+) cells

238 The portion of the TCR beta chain involved in antigen recognition in the sy

239 Diversity of the TCR beta chain is generated in part by a random yet intr

240 beta E6 motifs as the essential core of the TCR beta enhancer in pro-T cells.

241 hymic organs suppressed rearrangement of the TCR beta locus (but did not inhibit TCR gamma locus rear

242 Rearrangement of the TCR beta locus is normally required for development to t

243 on of a unique heterodimer consisting of the TCR beta-chain (TCRbeta) and a 33-kDa protein, FCp33.

244 and with particular variable regions of the TCR beta-chain (Vbeta).

245 expressing the variable gene segment of the TCR beta-chain 6 (Vbeta6) expanded in the spleens of mic

246 ally, we show that residue 29 in CDR1 of the TCR beta-chain affects recognition of the glutamic acid

247 recognition, in part similar to that of the TCR beta-chain and in part similar to the conventional a

248 ing size spectratyping and sequencing of the TCR beta-chain CDR3 region.

249 -ray structures, NMR characterization of the TCR beta-chain dynamics reveals significant chemical shi

250 on, the results showed that 20 to 30% of the TCR beta-chain gene (TCRB) sequences found in one joint

251 We show that the influence of the TCR beta-chain is due to a combination of Vbeta-, Jbeta-

252 ction in usage of the variable region of the TCR beta-chain over time.

253 Sequencing of the TCR beta-chain repertoire reveals that the DEJ CD8alphaa

254 MF; KF11) and identified common usage of the TCR beta-chain TRBV7 in eight of nine HLA B57 subjects e

255 and tumor regression through analysis of the TCR beta-chain V region gene products expressed in sampl

256 o far indicate increased interactions of the TCR beta-chain with the pMHC compared with their syngene

257 Typhimurium down-modulates expression of the TCR beta-chain, a molecule that is essential for Ag reco

258 rd complementarity-determining region of the TCR beta-chain.

259 ions) and an in vitro selection study on the TCR beta chain (four mutations).

260 To focus our analysis solely on the TCR beta-chain, we created a transgenic mouse expressing

261 which is consistent with the notion that the TCR beta-chain interacts in vivo preferentially with thi

262 of a particular mutant of SEC3 or SEB to the TCR beta chain, the greater its ability to stimulate T c

263 OVA-8:Kb complex C terminus overlap with the TCR beta-chain footprint, but that this footprint also e

264 ifferential regulation of regions within the TCR beta locus during thymocyte development.

265 , whereas recombination intermediates at the TCR-beta locus could be detected.

266 evelopment, and clonal rearrangements in the TCR-beta genes were present in most tumors.

267 heavy chain (IgH) locus, and delayed in the TCR-beta locus.

268 ZW) mice that have a natural deletion in the TCR-beta locus.

269 f the most prominently induced site near the TCR-beta enhancer (E beta) in allelic exclusion by targe

270 Proper assembly of the TCR-beta chain into the pre-TCR complex delivers signals

271 hich revealed an unexpected influence of the TCR-beta chain on the avidity of CD1d:alpha-GalCer bindi

272 erences conferred by the Vbeta domain of the TCR-beta chain, with Vbeta8.2 conferring higher avidity

273 intron sequences from another member of the TCR-beta family also triggered strong down-regulation, s

274 In-frame rearrangement of the TCR-beta locus and expression of the pre-TCR are compuls

275 hylation, and transcription in 100 kb of the TCR-beta locus.

276 on is determined by factors intrinsic to the TCR-beta locus.

277 mechanism for this downregulation using the TCR-beta gene, which acquires PTCs as a result of progra

278 n bacterial pneumonia, mice deleted of their TCR beta- and/or delta-chain were intratracheally inocul

279 ide stimulation and mRNA sequencing of their TCR beta-chains (TCRbeta).

280 N (or minor variations) in the CDR3 of their TCR beta-chains.

281 subcapsular zone where they recombine their TCR beta-chain and gamma-chain gene loci.

282 terrelated than cTreg subsets based on their TCR-beta repertoires, but exhibited varied immune profil

283 lineage, but we also demonstrated that this TCR beta-chain was able to provide stronger TCR signals.

284 This TCR-beta-driven mechanism would thus unify former per se

285 sion of this gene, which contains only three TCR-beta exons, exhibited efficient downregulation in re

286 combination and that in CD4+ CD8+ thymocytes TCR beta allelic exclusion does not result from inaccess

287 owever, when IL-7(-/-) thymi were grafted to TCR beta(-/-) mice, no development of graft-derived TCR

288 R beta-chain CDR3 regions were homologous to TCR beta-chains identified previously in allograft arter

289 x and p53 in thymocytes die at later ages to TCR-beta(-) or TCR-beta(+) thymic lymphomas containing a

290 ntroduced TCR alpha chain and the transgenic TCR beta chain from the original cytochrome c-specific T

291 We created mice expressing a transgenic TCR-beta chain that confers high affinity for self-lipid

292 e to monoclonal tumors with a single, unique TCR-beta chain and diverse TCR-alpha chains, pinpointing

293 We used TCR beta-chain nucleotide sequencing to gain insight int

294 Using TCR beta-chain immunosequencing, we observed that the pr

295 Experiments using TCR beta-chain knockout mice as BM donors indicated that

296 that express Valpha11 paired with non-Vbeta3 TCR beta-chains (Vbeta6, Vbeta8.1/8.2, Vbeta8.3, and Vbe

297 eptor (TCR) alpha chain paired with a Vbeta8 TCR beta chain in mice, or the homologous Valpha24-Jalph

298 inked glycosphingolipid specificity, whereas TCR beta-chains can confer heterogeneous additional reac

299 riant Valpha14-Jalpha281 TCR associated with TCR beta-chains of limited diversity.

300 GT(0) mice were crossed with TCR-beta knockout mice (TCR-beta(0)) to generate double-