ライフサイエンスコーパス: TFE

1 TFE also decreased the level of solubility of the peptid

2 TFE and ammonium perfluoro-octanoate exposures were high

3 TFE appears to induce the fibrils by stabilizing a beta-

4 TFE concentrations matching an alpha-helical content of

5 TFE increased the alpha-helical contribution of wild-typ

6 TFE operates via allosteric and direct mechanisms.

7 TFE unfolds VlsE at low percentages but promotes the for

8 TFE-fusion renal cell carcinomas (TFE-fusion RCCs) are c

9 TFE-induced conformational changes in the monomer protei

10 The addition of as little as 0.2% TFE increases aqueous spray stability not only in nESI d

11 Negative ion mode spray stability with 0.2% TFE is approximately 6x higher than for strictly aqueous

12 Upon addition of 0.2% TFE to the mobile phase of nLC/MS experiments, tryptic p

13 ert-butylphenyl, TFE-d(3) = CF(3)CD(2)OD) (2(TFE)) were determined.

14 Addition of methanol to solutions of 2(TFE) rapidly establishes equilibrium between methanol (2

15 vation kinetics were conducted by reacting 2(TFE)-(13)C with 300-1000 psi of methane in single-crysta

16 n methanol (2(MeOD)) and trifluoroethanol (2(TFE)) adducts, with methanol binding preferentially (K(e

17 Reaction of dimethyl ether with 2(TFE) proceeds similarly (K(eq) = 0.023 +/- 0.002, 313 K;

18 han Leu, Ile, and Val at 50 degrees C in 20% TFE.

19 be used to extrapolate the results from 25% TFE (approximately 4 M) back to water.

20 role in the mechanism of aggregation in 25% TFE, but also from mutations located in other regions.

21 propensity increases regularly from 0 to 25% TFE but levels off at higher TFE concentrations, which e

22 In 30% TFE, all analogs reached a maximal helical content of 80

23 107-111 are considerably more ordered in 30% TFE.

24 signments of monomeric pEAbeta (3-42) in 40% TFE solution.

25 studies of both peptides in saturating (43%) TFE reveal stable alpha-helices from Gly500 to Lys522, b

26 e disaggregation under milder conditions (5% TFE).

27 the sample to give a solution containing 5% TFE, the fraction of partially unfolded monomeric protei

28 rate of disaggregation of protofibrils in 5% TFE result not only from mutations situated in the regio

29 gh-resolution structures obtained with 50:50 TFE/water revealed that all three analogs display two he

30 +) in the presence of the CTAB than in a 50% TFE solution (K(d) = 3.1 x 10(-4) M in CTAB and 2.3 x 10

31 tion of extended structures in water and 50% TFE solutions.

32 dmol(-1), in water at 2 degrees C and in 50% TFE at 2 degrees C, respectively.

33 /- 2200 deg cm2 dmol(-1) in water and in 50% TFE at 2 degrees C.

34 [theta]222 for a 100% helix, NLEKG14 in 50% TFE at 25 degrees C was estimated to be 77% helix.

35 t thermal denaturation of the peptide in 50% TFE containing 1 mM Zn(2+) was associated with both enth

36 te calcium binding (K(d) = 170 microM in 50% TFE).

37 In 50% TFE, the peptide had a CD spectrum consistent with an al

38 15)N- and (15)N/(13)C-labeled peptide in 50% TFE.

39 aqueous buffers, and monomeric forms in 50% TFE.

40 dimensional 1H NMR studies of NLEKG14 in 50% TFE.

41 In 50% TFE/H(2)O, M2-30 assumed a beta-like structure.

42 Circular dichroism (CD) studies in 50% TFE/H2O revealed a predominantly helical conformation fo

43 In 90 % TFE, the beta-turn fraction is estimated to be about 75

44 are ordered to a significant extent in 90 % TFE.

45 d the same CD patterns as those found in 99% TFE/H(2)O.

46 ween spin labels located at i and i + 4 in a TFE/H(2)O mixture or a POPC bilayer is indicative of an

47 B(1)(T377-E416) peptide reconstituted into a TFE/H(2)O mixture or a POPC or DMPC bilayer were estimat

48 in the presence of the helix-inducing agent TFE.

49 ghest helix propensity at 0 degrees C in all TFE concentrations, it is lower than Leu, Ile, and Val a

50 Although TFE destabilizes native holomyoglobin, as well as native

51 uch as c-myb, members of the ets family, and TFE-III.

52 For cpn10, both MeOH and TFE additions govern initial unfolding; however, further

53 nt (kH) were measured on going from MeOH and TFE to isooctane (kH(isooctane)/kH(MeOH) = 5-12; kH(isoo

54 rillization kinetics depended on peptide and TFE concentrations, and had a nucleation step followed b

55 eptides form fibrils in aqueous solution and TFE disrupts these fibrils.

56 he higher reaction rates observed in TFA and TFE compared with CH2Cl2 arise from stabilization of the

57 d phylogenetic distribution of TBP, TFB, and TFE transcription factors, and

58 ater, 2,2,2-trifluoroethanol(TFE)/water, and TFE/ethanol mixtures.

59 ated in water and fully populated in aqueous TFE.

60 entification of the beta-subunit of archaeal TFE enabled us to reconstruct the evolutionary history o

61 major role with strong HBD solvents such as TFE.

62 investigate the causal relationship between TFE-induced structural transitions and aggregation.

63 ees C in D2O and at room temperature in both TFE and H2O.

64 we introduce (19)F, in this case from bound TFE, as a new probe for the binding of small molecules t

65 The (19)F and (2)H ENDOR spectra of bound TFE together with (1,2)H ENDOR spectra of bound ethanol

66 Me)-C(Me)=NAr; Ar = 3,5-di-tert-butylphenyl, TFE-d(3) = CF(3)CD(2)OD) (2(TFE)) were determined.

67 e) C(Me) N Ar; Ar = 3,5-di-tert-butylphenyl; TFE-d(3) = CF(3)CD(2)OD) were studied.

68 The residues most desolvated by TFE are the alanines located at position i - 4 in the se

69 of helix induced in the peptide fragments by TFE varied.

70 so give curves of helix formation induced by TFE at constant temperature, and the properties of these

71 e slow structural change of Con A induced by TFE provides a useful model system for study of protein

72 ic disorder that is substantially reduced by TFE, but a significant gradient in dynamics is observed,

73 ning of the RNAP clamp that is stimulated by TFE.

74 cular hydrogen bonds are not strengthened by TFE and that amide hydrogen bonds in the transition stat

75 TFE-fusion renal cell carcinomas (TFE-fusion RCCs) are caused by chromosomal translocation

76 lkene difluorocyclopropanation and competing TFE/c-C(3)F(6)/homologous perfluoroanion generation, (13

77 of thermal unfolding curves in concentrated TFE solutions results from the decrease of the enthalpy

78 At low concentrations, TFE destabilizes the unfolded species and thereby indire

79 The CFC-113 degradation intermediates CTFE, TFE, and cis-DFE did not inhibit TCE dechlorination by D

80 f a transcription activator, here designated TFE, that may be universally present in the Archaea.

81 During elongation, Spt4/5 can displace TFE from the RNAP elongation complex and stimulate proce

82 It does, however, displace TFE from the diiron site in H(mv).

83 tion factor B (TFB), transcription factor E (TFE) and the 12-subunit RNA polymerase (RNAP) from Metha

84 artially relieved by transcription factor E (TFE).

85 anisms of the basal transcription factors E (TFE) and Spt4/5 through conformational constraints has r

86 lculations indicate that the solvent, either TFE or HFIP, can stabilize the transition state through

87 ive to backbone fluctuations and that either TFE or calcium binding stabilizes the secondary structur

88 ) working electrodes: a thin-film electrode (TFE), a screen-printed electrode (SPE), and a microarray

89 it, via a through-fall exclusion experiment (TFE) in an eastern Amazonian rainforest.

90 The archaeal initiation factor TFE and its eukaryotic counterpart TFIIE facilitate this

91 nd also subunit E and a transcription factor TFE that co-purifies with RNAP from wild-type cells, but

92 ctions with the general transcription factor TFE, as well as with the transcriptional activator Ptr2.

93 nated alcohol varied from 53:47 to 87:13 for TFE, 60:40 to 92:8 for HFP, and 52:48 to 73:27 for PFTB.

94 emained unchanged ( approximately 40:60) for TFE and for PFTB over the range of 25-250 molar equiv.

95 xposure matrix (1950-2002) was developed for TFE and ammonium perfluoro-octanoate, a chemical used in

96 such a term greatly improves the fitting for TFE, MeCN/H(2)O 2:1, and MeOH but at the expense of that

97 rmylphenylalanine trifluoroethyl ester (fPhe-TFE) represents an improvement over earlier model reacti

98 ncatalyzed reaction of glycinamide with fPhe-TFE proceeds with a second-order rate constant of 3 x 10

99 he helix-forming properties of peptides from TFE/H2O mixtures (TFE = 2,2, 2-trifluoroethanol) back to

100 On the other hand, TFE destabilizes native proteins, as we confirm here, pr

101 a highly alpha-helical conformation at high TFE and hexafluoro-2-propanol concentrations.

102 a highly alpha-helical conformation at high [TFE].

103 y from 0 to 25% TFE but levels off at higher TFE concentrations, which explains why the extent of hel

104 nthalpy change for helix unfolding at higher TFE concentrations.

105 TFIIE and the archaeal homolog TFE enhance DNA strand separation of eukaryotic RNAPII a

106 However, TFE is unable to promote the propagation of helix beyond

107 In TFE, apoA-V(296-343) adopts an extended amphipathic alph

108 In TFE, MeCN/H(2)O 2:1, and MeOH, the measured k(H) values

109 MR studies performed on elbow and elbow-A in TFE indicate that the helical structure is confined to t

110 Con A in TFE provides an example of an intermediate with non-nati

111 clude that initiation of EGFP aggregation in TFE likely involves overcoming of multiple protective fa

112 -residue peptides in aqueous solution and in TFE/water mixtures.

113 water, but Ser2 affects the conformation in TFE-rich solution in much the same way as Ser5-->Ala sub

114 ent in water and affects the conformation in TFE-rich solutions.

115 lical structures in the peptide fragments in TFE was correlated with the observation of turn and/or h

116 gment with the highest degree of helicity in TFE corresponded with the single (alpha-helix in native

117 ing with beta-strand 1 did not form helix in TFE.

118 3.1 x 10(-4) M in CTAB and 2.3 x 10(-4) M in TFE).

119 mpound, salicylic acid, has been measured in TFE/H2O mixtures from the pKa difference between salicyl

120 elix in the D-helix region of the peptide in TFE.

121 the increase in average helix propensity in TFE/H2O mixtures.

122 of different solvents (e.g., 99.6% ee (R) in TFE vs 71.2% ee (S) in methyl ethyl ketone).

123 ed in the C-terminal sequence is retained in TFE.

124 hat helix formation is basically the same in TFE/H2O mixtures as in water.

125 e peptides show an increase in beta-sheet in TFE, a known inducer of alpha-helices, relative to that

126 asing entropic costs of protein solvation in TFE-water mixtures may both cause the population of the

127 sed propensity to form helical structures in TFE also failed to stimulate I(SC).

128 of hydrogen bond strength versus increasing TFE concentration matches both in shape and magnitude th

129 isooctane)/kH(MeOH) = 5-12; kH(isooctane)/kH(TFE) > 80).

130 m with the natively disordered state at low [TFE] and with a highly alpha-helical conformation at hig

131 Microphthalmia/TFE (MiT) transcription factors (TFs), such as transcrip

132 e microphthalmia/transcription factor E (MiT/TFE) family, are similarly regulated during mitophagy.

133 30 (hlh-30), the Caenorhabditis elegans MiT/TFE ortholog, to starvation followed by refeeding to und

134 expression of the transcription factors MiT/TFE and FOXH1, and that of lysosomal and autophagy genes

135 d a resulting suppression of AKT-induced MiT/TFE downregulation.

136 Up-regulation of MiT/TFE genes in cells and tissues from patients and murine

137 f mTORC1 loss-of-function fully restored MiT/TFE expression and activity.

138 We found that MiT/TFE transcription factors-master regulators of lysosomal

139 global metabolite profiling reveals that MiT/TFE-dependent autophagy-lysosome activation is specifica

140 es growth and suppresses activity of the MiT/TFE family of transcription factors that control biogene

141 and nutrient scavenging, mediated by the MiT/TFE family of transcription factors.

142 enes, granting promoter occupancy to the MiT/TFE members, TFEB and TFE3, and/or the autophagy regulat

143 These results identify the MiT/TFE proteins as master regulators of metabolic reprogram

144 In human PDA cells, the MiT/TFE proteins--MITF, TFE3 and TFEB--are decoupled from re

145 results illuminate a pathway leading to MiT/TFE transcription factor activation, distinct from starv

146 suggesting cross talk between these two MiT/TFE activation pathways.

147 1 required for a direct interaction of MITF-TFE factors and E-box 2 for binding of the as yet uniden

148 cently demonstrated that members of the MITF-TFE family modulate BEST1 transcription.

149 e suggest that VMD2 is regulated by the MITF-TFE family through two E-boxes, with E-box 1 required fo

150 ctor EB (TFEB) and other members of the MiTF/TFE family of transcription factors through association

151 ive activation of TFEB, a member of the MITF/TFE family of transcription factors.

152 roperties of peptides from TFE/H2O mixtures (TFE = 2,2, 2-trifluoroethanol) back to water, the therma

153 e lysozyme and ribonuclease A, nevertheless, TFE stabilizes native apomyoglobin.

154 Deacylations with Li+ -OCH2CF3/TFE proceed at ambient temperature (or with mild heating

155 ter-directed transcription in the absence of TFE, which alleviates this effect by displacing Spt4/5 f

156 for Abeta fibril formation in the absence of TFE.

157 ibrils in aqueous solution in the absence of TFE.

158 erminus, both in the presence and absence of TFE.

159 brium CD results show that, upon addition of TFE, low-concentration Con A transforms to a highly alph

160 a-structure, whereas subsequent additions of TFE induce a superhelical structure.

161 As the amount of TFE is increased above 20%, helix content progressively

162 Moreover, low concentrations of TFE compensate for helix-destabilizing mutations in the

163 s the rate of folding, low concentrations of TFE increase the rate of folding.

164 Low concentrations of TFE strongly stabilize the pH 4 folding intermediate.

165 In the presence of low concentrations of TFE, fibril formation is observed in Abeta samples at na

166 eviews that extensively report copolymers of TFE (listed below).

167 TFE) followed by reductive defluorination of TFE to cis-1,2-difluoroethene (cis-DFE) as an end produc

168 Displacement of TFE by a C-H bond appears to be the rate-determining ste

169 mine whether the helix-stabilizing effect of TFE arises from strengthening the hydrogen bonds in the

170 We ask which effect of TFE dominates in this case: strengthening helices or wea

171 que well suited for addressing the effect of TFE on polypeptide conformation.

172 hobic interaction, is the dominant effect of TFE on the folding intermediate.

173 o the DEs (1 or 2) in over 25 molar equiv of TFE occurred highly stereoselectively to afford only cis

174 r, we report the IR spectra as a function of TFE concentration for an alanine-rich peptide based on t

175 s to reconstruct the evolutionary history of TFE/TFIIE-like factors, which is characterised by winged

176 es specifically on the homopolymerization of TFE (the starting point for all fluoropolymer industries

177 ysical studies have revealed the position of TFE/TFIIE within the pre-initiation complex (PIC) and il

178 ion of 2D NMR experiments in the presence of TFE or DPC micelles, complete 1H NMR assignments of the

179 ecise residues desolvated in the presence of TFE were identified.

180 pendent of the ionic strength or presence of TFE, as judged by FTIR.

181 D) to analyze the CD data in the presence of TFE, by fully assigning the unbound IA(3) protein by NMR

182 (3) become more dispersed in the presence of TFE, indicating that the protein undergoes an unstructur

183 ndergo additional folding in the presence of TFE.

184 to structured transition in the presence of TFE.

185 iscussion on the synthesis and production of TFE (both at industrial and laboratory scales), includin

186 f acylphosphatase by monitoring the range of TFE concentrations that result in aggregation.

187 Our results support the use of TFE as an empirical probe of hidden structural propensit

188 a linear dependence of ln <w> and DeltaH on TFE molarity can be used to extrapolate the results from

189 zene) with SmI(2) in the presence of MeOH or TFE was studied.

190 ially alter the CTD conformation in water or TFE.

191 ere measured between 0 and 50 volume percent TFE and were fitted to the modified Lifson-Roig theory.

192 arrows the range of uncertainty on potential TFE carcinogenicity but cannot conclusively confirm or r

193 hemours, Juhua, 3F, 3M/Dyneon, etc., produce TFE homopolymers.

194 V-1 templates with the E box-binding protein TFE-3.

195 , methanol, and dimethyl ether by [(N-N)PtMe(TFE-d(3))](+) ((N-N) = ArN=C(Me)-C(Me)=NAr; Ar = 3,5-di-

196 luoroethanol (TFE) for the generation of PVP/TFE pockets on the surface of a PCL jet.

197 Rather, TFE increases the structure of the binary alcohol/water

198 alpha-helix in the 25-mer even in saturating TFE.

199 Secondly, TFE binds physically to single-stranded DNA in the trans

200 In the presence of the organic solvent TFE, the conformation of the pentamer changes from PII t

201 benzene substitution proceeds by a solvent (TFE)-assisted associative pathway.

202 Tetrafluoroethylene (TFE), a compound used for the production of fluorinated

203 uoroethylene (CTFE) and tetrafluoroethylene (TFE) were determined in the temperature range 240-340 de

204 e homopolymerization of tetrafluoroethylene (TFE), detailing the TFE homopolymerization process and t

205 In TFA/TFE mixtures, 2 and 3 are in equilibrium with a slight t

206 mplex, consisting of promoter DNA, TBP, TFB, TFE, and RNAP.

207 s, is important in promoter opening and that TFE can compensate for defects in the N terminus through

208 The IR spectra confirm that TFE desolvates the helical state of the peptide to a gre

209 sively confirm or refute the hypothesis that TFE is carcinogenic to humans.

210 uncation mutant alphaS1-102, indicating that TFE-induced structural transitions involve the N terminu

211 We propose that TFE and the bacterial general transcription factor CarD,

212 Several recent papers have proposed that TFE acts by selectively desolvating the peptide backbone

213 The TFE effect is concentration dependent and is maximal at

214 able than that in the shorter peptide as the TFE concentration increases.

215 g site with few intermolecular contacts, the TFE-inducible alphaB motif is deeply engaged in a hydrop

216 of tetrafluoroethylene (TFE), detailing the TFE homopolymerization process and the resulting chemica

217 Current density was lowest for the TFE and SPE surfaces (linear diffusion), higher for the

218 ensive secondary structure is present in the TFE-denatured state but not in the protein denatured in

219 f dark respiration (Rd ) was elevated in the TFE-treated forest trees relative to the control by 28.2

220 The interaction sites of the TFE WH domain and the transcription elongation factor Sp

221 We have localized the position of the TFE winged helix (WH) and Zinc ribbon (ZR) domains on th

222 ) to elucidate both the main features of the TFE-driven transition and the residue-level deviations f

223 ly helical intermediate is on-pathway to the TFE-induced formation of both the highly helical monomer

224 TFE fibrils is strongly correlated with the TFE-induced formation of a monomeric, partly helical int

225 tates upon association with membranes, these TFE-induced conformations imply relevant pathways for me

226 We find that efficient production of these TFE fibrils is strongly correlated with the TFE-induced

227 This TFE-induced fibrillization is quite unusual, because mos

228 In contrast to TFE, the conformational transition of the 1-500 fragment

229 Among 4,773 workers ever exposed to TFE, we found a lower rate of death from most causes, as

230 d trend (P = 0.24) by cumulative exposure to TFE was observed for liver cancer.

231 Detergent micelles were superior to TFE in their ability to induce secondary structural frac

232 Trifluoroethanol (TFE) stabilized a native-like beta-turn in BH(9-10).

233 Trifluoroethanol (TFE) stabilized the secondary structure of the apoE CT d

234 nding of ethanol and 1,1,1-trifluoroethanol (TFE) to both the H(mv) and H(ox) forms of soluble methan

235 egation in 25% (v/v) 2,2,2 trifluoroethanol (TFE) are different from those that promote disaggregatio

236 cilitated by solvent 2,2,2-trifluoroethanol (TFE) acting as a proton shuttle.

237 opropanol (HFIP) and 2,2,2-trifluoroethanol (TFE) as reaction media is described.

238 ow concentrations of 2,2,2-trifluoroethanol (TFE) convert predominately unstructured Abeta monomers i

239 yrrolidone) (PVP) in 2,2,2-trifluoroethanol (TFE) for the generation of PVP/TFE pockets on the surfac

240 formed in 60% (v/v) 2,2,2-trifluoroethanol (TFE) has been studied using hydrogen exchange pulse labe

241 solutions containing 2,2,2-trifluoroethanol (TFE) have shown that the formation of structural interme

242 2,2,2-Trifluoroethanol (TFE) is known to stabilize peptide helices by strengthen

243 2,2,2-Trifluoroethanol (TFE) is widely used to induce helix formation in peptide

244 2,2,2-Trifluoroethanol (TFE) most stabilizes the alpha-helix-like conformations,

245 1 x 2 (CF3CH2O-) in 2,2,2-trifluoroethanol (TFE) or lithium trifluoroethoxide/TFE.

246 in water and in 23% 2,2,2-trifluoroethanol (TFE) shows that the individual residue cross peaks of IA

247 queous buffer and in 2,2,2-trifluoroethanol (TFE) using CD and NMR spectroscopy.

248 n in the presence of 2,2,2-trifluoroethanol (TFE), a solvent known to stabilize hydrogen bonds within

249 osolvents, including 2,2,2-trifluoroethanol (TFE), on the thermodynamics and kinetics of folding of t

250 , residues 20-29) in 2,2,2-trifluoroethanol (TFE).

251 ylbenzene (tBuPh) to 2,2,2-trifluoroethanol (TFE).

252 v) concentrations of 2,2,2-trifluoroethanol (TFE).

253 solvent system (40% 2,2,2-Trifluoroethanol (TFE)/water), which gave the largest structural differenc

254 pha-helical content in 30% trifluoroethanol (TFE) or in dodecylphosphocholine (DPC) micelles, which m

255 ed to the structure in 30% trifluoroethanol (TFE), and clear differences were observed.

256 ndary structure, while 40% trifluoroethanol (TFE) induces >90% helicity and is unperturbed by the spi

257 dered but inducible in 40% trifluoroethanol (TFE).

258 duced by dissolving in 50% trifluoroethanol (TFE) were monitored at neutral and low pH by far- and ne

259 econdary structures in 99% trifluoroethanol (TFE)/H(2)O.

260 n the fluorinated alcohols trifluoroethanol (TFE), hexafluoropropan-2-ol (HFP), and perfluoro-tert-bu

261 uoroisopropanol (HFIP) and trifluoroethanol (TFE), to activate the electron-deficient heterocyclic az

262 oluble in SDS micelles and trifluoroethanol (TFE).

263 organic solvents, such as trifluoroethanol (TFE) and methanol (MeOH), indicating a lower propensity

264 ich conformation driven by trifluoroethanol (TFE).

265 ueous solutions containing trifluoroethanol (TFE).

266 lar dichroism (CD) in H2O, trifluoroethanol (TFE), and SDS micelles confirmed the importance of the a

267 dichroism (CD) spectra in trifluoroethanol (TFE) solution are obtained.

268 In trifluoroethanol (TFE), Au(OAc(F))(CH2CH2OCH2CF3)(tpy) (3) is formed as th

269 We describe a novel trifluoroethanol (TFE) or hexafluoropropan-2-ol (HFP) mediated substitutio

270 as well as the ability of trifluoroethanol (TFE) and detergent systems to induce secondary structure

271 ns, and in the presence of trifluoroethanol (TFE) and dodecylphosphocholine (DPC) micelles.

272 mode by trace addition of trifluoroethanol (TFE) to aqueous samples.

273 itions via the addition of trifluoroethanol (TFE), DMSO, DMF and acetone, uniform fiber-like nanopart

274 d in vitro, by addition of trifluoroethanol (TFE), into amyloid fibrils of the type observed in a ran

275 moderate concentrations of trifluoroethanol (TFE), or because of the appearance of a highly alpha-hel

276 Upon addition of trifluoroethanol (TFE), significant shifts are observed in a number of res

277 ing and in the presence of trifluoroethanol (TFE).

278 ide (TMAO) and the solvent trifluoroethanol (TFE).

279 of the helicogenic solvent trifluoroethanol (TFE).

280 gen bond-promoting solvent trifluoroethanol (TFE).

281 the lipid mimetic solvent trifluoroethanol (TFE; 50% v/v).

282 the presence of 25% (v/v) trifluoroethanol (TFE) AcP undergoes partial unfolding and globular aggreg

283 In 30 vol-% trifluoroethanol (TFE), a single continuous helix is evident in a signific

284 acid chlorides in 97% w/w trifluoroethanol (TFE)-water spanning over 10 (9) in rate constant at 25 d

285 sured by using CD in water/trifluoroethanol (TFE) mixtures.

286 YGR, in water and in water/trifluoroethanol (TFE).

287 ses in acetone/water, 2,2,2-trifluoroethanol(TFE)/water, and TFE/ethanol mixtures.

288 OH) and fluorinated (i.e., trifluoroethanol, TFE) alcohols on the secondary structure and thermodynam

289 tion of CFC-113 to CTFE and trifluoroethene (TFE) followed by reductive defluorination of TFE to cis-

290 roethanol (TFE) or lithium trifluoroethoxide/TFE.

291 shed new light on the mechanisms underlying TFE-fusion RCCs and suggest a possible therapeutic strat

292 n kinetic behavior upon addition of 5% (v/v) TFE indicates that it stabilizes the transition state to

293 ta (1-42), we started our studies in various TFE-water mixtures and found striking differences betwee

294 ns where the peptides are most folded (water/TFE, 5 degrees C), tau(ex) values for all residues in ea

295 oism and by 2D-NMR in the presence of water; TFE/water; SDS micelles; and in the presence of both neu

296 Thus, the mechanism by which TFE exerts its helix-stabilizing effects can be divorced

297 circular dichroism data to a model in which TFE-water mixtures are assumed to be ideal solutions, we

298 We also found that while TFE induces more alpha-helices, it favors multiple, shor

299 is latter intermediate at -78 degrees C with TFE occurs selectively at the vinyl CH(2) closer to the

300 rring after rapid mixing of the protein with TFE.